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All Journal Jurnal Fitopatologi Indonesia Bioma : Berkala Ilmiah Biologi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Hortikultura JURNAL AGROTEKNOLOGI AGRIVITA, Journal of Agricultural Science Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Penelitian Pertanian Tanaman Pangan Biosaintifika: Journal of Biology & Biology Education Jurnal Entomologi Indonesia Jurnal Kedokteran Hewan Jurnal Ilmu-Ilmu Pertanian

Y. Andi Trisyono

Fakultas Pertanian Universitas Gadjah Mada, Yogyakarta, Indonesia

Author-ID : 3094337

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Medicine & Pharmacology Veterinary

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Pola Sebaran Kelompok Telur Ostrinia furnacalis Guenée (Lepidoptera: Crambidae) pada Beberapa Fase Pertumbuhan Tanaman Jagung (Zea mays L.) Subiadi Subiadi; Y. Andi Trisyono; Edhi Martono
Jurnal Perlindungan Tanaman Indonesia Vol 18, No 1 (2014)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.15573

A field study was conducted to determine the distribution of egg masses of Ostrinia furnacalis on corn leaves and within corn field. The corn field was divided into three regions: the inner edge closed to other crops and outer edge closed to main road and the middle region. The numbers of egg masses laid were recorded entirely in all corn plants existed (census method). Egg laying period lasted for 34 days, with 11 days from initiation of egg laying to the peak of oviposition and 23 days from the peak to termination of egg laying. The egg masses laid on corn leaves were distributed in aggregation pattern. During eight-opened-leaf to twelve-opened-leaf stages, there were 847 egg masses found, and 80.9% was laid on the sixth-to-ninth leaves. From tasseling to blister stages there were 491 egg masses found of which approximately 80.7% was laid on the seventh-to-11 32.8, and 29.8% of those were found in the inner edge, middle, and outer edge of corn field, respectively. leaves. Egg masses laid within corn field varied, in which 37.4, 32.8, and 29.8% of those were found in the inner edge, middle, and outer edge of corn field, respectively. Kajian lapang dilaksanakan untuk mengetahui pola sebaran peletakan kelompok telur ngengat Ostrinia furnacalis pada daun tanaman jagung dan penyebarannya pada lahan pertanaman jagung. Lahan penelitian dibagi menjadi tiga kelompok yaitu lahan pinggir dalam berdekatan dengan pertanaman lain, bagian tengah lahan, dan lahan pinggir luar berdekatan dengan jalan raya. Pengamatan kelompok telur dilakukan pada seluruh tanaman jagung (sensus). Periode peletakan telur berlangsung selama 34 hari, dengan periode inisiasi hingga puncak 11 hari dan periode setelah puncak hingga akhir peletakan telur 23 hari. Peletakan telur O. furnacalis pada daun tanaman jagung menyebar secara berkelompok. Pada fase 8 hingga 10 daun telah terbuka sempurna ditemukan sebanyak 847 kelompok telur, 80,9% diantaranya ditemukan pada daun ke 6–9. Pada fase VT (bunga jantan) hingga fase R2 (bunga betina telah kering) ditemukan sebanyak 491 kelompok telur, 80,7% diantaranya ditemukan pada daun 7–11. Penyebaran kelompok telur pada bagian lahan terjadi secara berkelompok. Jumlah kelompok telur tertinggi berturut-turut dari bagian lahan pinggir dalam, bagian tengah lahan, dan bagian lahan pinggir luar masing-masing 37,4; 32,8; dan 29,8%.

The Effect of Bacillus thuringiensis toxin Cry1A.105 and Cry2Ab2 on The Survival of The Non-Target Pest, Spodoptera litura Kurnia Pratiwi; Y. Andi Trisyono; Edhi Martono
Jurnal Perlindungan Tanaman Indonesia Vol 20, No 1 (2016)
Publisher : Universitas Gadjah Mada

Spodoptera litura is one of the important insect pest of maize besides the notoriously damaging corn borer, Ostrinia furnacalis. S. litura has been the target of various controls including the use of Bacillus thuringiensis (Bt) toxin Cry1A.105 and Cry2Ab2. This study was conducted to evaluate the acute effect of Bt toxin Cry1A.105 and Cry2Ab2 on the growth and development of S. litura from larval to adult stages. Two sublethal concentrations were used; 0.1875 and 0.0469 ppm for Cry1A.105, and 0.0008 and 0.0003 ppm for Cry2Ab2. The bioassay using diet dipping was carried out on a CRD with three experiments and five repetitions. The observation was carried out on the mortality and survival rates of S. litura. The mortality reached 28% when the larvae were treated with 0.1875 ppm and 20% with 0.0469 ppm of Cry 1A.105. The exposed larvae and pupae were smaller than control. Larval and pupal weight were 117.0 and 165.6 g with 0.1875 ppm, while control were 212.9 and 211.2 g. Cry1A.105 also longer the larval stage, larval stage with higher and lower concentration were 24.5 and 22.3 day, while control was 20.5 day. The resulted pupae from larve which exposed by Cry1A.105 were less than control; there were 40% at concentration 0.1875 ppm and control 61%. The two concentration of Cry2Ab2 produced similar mortality of 20%. Similarly, Cry2Ab2 affected pupal to adult stages development. The longevity of pupal stage with concentration 0.0003 ppm was 9.5 days, followed by 0.0008 ppm (9.1 days) and control (10.1 days). The adult emerge on the highest concentration was 47.4% while control only 34.6%. There results showed that both Cry1A.105 and Cry2Ab2 were detrimental to the survival of S. litura which is the non-target insect of transgenic Bt maize. INTISARI Spodoptera litura merupakan salah satu hama penting yang menyerang tanaman jagung, selain Ostrinia furnacalis. Belakangan ini O. furnacalis diketahui telah menjadi target dari berbagai macam cara pengendalian termasuk penggunaan toksin Bacillus thuringiensis (Bt) Cry1A.105 dan Cry2Ab2. Penelitian ini dilakukan untuk mengetahui efek akut toksin Bt Cry1A.105 dan Cry2Ab2 terhadap pertumbuhan dan perkembangan S. litura dari larva sampai imago. Dua konsentrasi subletal yang akan digunakan adalah; 0,1875 dan 0,0469 ppm untuk Cry1A.105, dan 0,0008 dan 0,0003 ppm untuk Cry2Ab2. Pengujian dilakukan dengan menggunakan metode celup pakan dan Rancangan Acak Legkap dengan tiga perlakuan dan lima ulangan. Pengamatan dilakukan terhadap mortalitas dan kelangsungan hidup S. litura. Mortalitas mencapai 28% pada larva yang dipaparkan dengan 0,1875 ppm dan 20% dengan 0,0469 ppm Cry 1A.105. Larva dan pupa yang terkena toksin berukuran lebih kecil. Berat larva dan pupa yang terpapar toksin dengan konsentrasi 0,1875 ppm, masing – masing 117,0 dan 165,6 g, sedangkan kontrol masing – masing 212,9 dan 211,2 g. Cry1A.105 juga dapat memperpanjang stadia larva. Lama stadia larva dengan konsentrasi tertinggi dan terendah adalah 24,5 dan 22,3 hari, sedangkan kontrol 20,5 hari. Jumlah pupa yang berhasil terbentuk dari larva yang terpapar toxin Cry1A.105 lebih sedikit dibandingkan dengan kontrol; pada konsentrasi 0,1875 ppm sebesar 40%, sedangkan kontrol sebesar 61%. Kedua konsentrasi dari toksin Bt Cry2Ab2 menyebabkan mortalitas yang sama yaitu 20%. Cry2Ab2 juga berpengaruh terhadap lama stadia pupa dan tingkat keberhasilan pembentukan imago. Lama stadia pupa dengan konsentrasi 0,0003 ppm adalah 9,5 hari, diikuti dengan konsentrasi 0,0008 ppm (9,1 hari) dan kontrol (10,1 hari). Jumlah imago terbanyak terdapat pada perlakuan dengan konsentrasi 0,0008 ppm sebesar 47,4% sedangkan pada kontrol hanya 34,6%. Hal ini menunjukkan bahwa toksin Bt Cry1A. 105 dan Cry2Ab2 juga berpengaruh terhadap kelangsungan hidup S. litura yang merupakan serangga bukan sasaran dari tanaman jagung transgenik Bt.

Preferensi Ngengat Citripestis sagitiferella terhadap Minyak Atsiri Tiga Varietas Jeruk - Muryati; Y A Trisyono; - Witjaksono
Jurnal Hortikultura Vol 15, No 1 (2005): Maret 2005
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v15n1.2005.p%p

Penelitian ini bertujuan untuk mengetahui preferensi ngengat Citripestis sagitiferella terhadap minyakatsiri yang diekstrak dari tiga varietas jeruk (manis pacitan, besar nambangan, dan siem). Buah jeruk yang digunakansebagai materi untuk ekstraksi diambil dari Batu, Malang (untuk jeruk manis dan siem) dan Magetan (untuk jerukbesar) pada bulan Juli 2001. Minyak atsiri jeruk diperoleh dengan metode destilasi uap air. Ngengat C. sagitiferellayang digunakan untuk perlakuan diperoleh dengan mengumpulkan larva yang menyerang jeruk manis di Batu Malangpada bulan Oktober 2001, kemudian buah yang terserang dibawa ke laboratorium Ilmu Hama Tumbuhan FakultasPertanian Universitas Gadjah Mada, Yogyakarta untuk dipelihara sampai menjadi ngengat. Ngengat yang barumuncul kemudian digunakan untuk uji preferensi menggunakan olfaktometer yang berbentuk Y dan terbuat daritabung kaca py rex. Untuk mengetahui komposisi senyawa kimia dari minyak atsiri jeruk, dilakukan deteksimenggunakan kromatografi gas dan kromatografi gas-spektrofotometri massa. Hasil penelitian menunjukkan bahwangengat C. sagitiferella tertarik pada minyak atsiri dari ketiga varietas jeruk. Tingkat preferensi C. sagitiferellatertinggi adalah terhadap minyak atsiri dari jeruk manis, dan selanjutnya jeruk besar dan siem. Minyak atsiri yangdiekstrak dari jeruk manis yang berumur 6 bulan lebih menarik ngengat C. sagitiferella dibandingkan yang berumur 2bulan. The pref er ence of Citripestis sagitiferella mothsto vol a tile com pounds of three cit rus va ri et ies. The ob jec tive of this re search was to de ter mine the pref er ence ofC. sagitiferella moths to vol a tile com pounds ex tracted from three cit rus va ri et ies (pacitan sweet or ange, nambanganpom elo, and siem or ange). Citrus fruits were taken from Batu, Malang (sweet or ange and siem or ange) and Magetan(pom elo) in July 2001. The vol a tile com pounds were ob tained through a steam distilation method. The lar vae of C.sagitiferella were col lected from dam aged sweet or ange (Batu, Malang) in Oc to ber 2001, and brought to the lab o ra -tory for sub se quent de vel op ment. Newly emerged moths were used in this study. The pref er ence tests were car ried outus ing a Y shape olfactometer. The GC and GC-MS were used to de tect the com po nents of vol a tile com pounds. There sults showed that C. sagitiferella moths at tracted to vol a tile com pounds ex tracted from all cit rus va ri et ies tested,with sweet or ange was found to be the most pref er a ble, fol lowed by pom elo and siem or ange. Fur ther more, the vol a tilecom pounds ex tracted from 6 months (af ter fruit set ting) sweet or ange was more at trac tive for C. sagitiferella mothsthen the 2 months (af ter fruit set ting) or ange fruit.

Oviposition Deterrent of Bactrocera carambolae Resulted from Eggs Deposition on Mango Muryati Muryati; Y. Andi Trisyono; Witjaksono Witjaksono; Wahyono Wahyono
AGRIVITA, Journal of Agricultural Science Vol 39, No 2 (2017): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v39i2.1097

Oviposition deterrent is chemical compounds which are used for avoiding eggs deposition. The oviposition deterrent resulted from eggs deposition is valuable information that can be manipulated for managing its population. The objective of this research was to determine the presence of oviposition deterrent resulted by female Bactrocera carambolae on mango. Extraction of oviposition deterrent was conducted by maceration method. The preference test was performed using two arms olfactometer, meanwhile the oviposition deterrent test was conducted by exposed gravid females to fruit that already smeared with extracts. The result revealed that gravid females of B. carambolae were attracted to methanol extract of 1 day after egg deposition, whereas the methanol extract of 3 and 5 days after egg deposition repelled gravid females. Oviposition deterrent test indicated that methanol extract at category 3 and 5 acted as a deterrent. The preference of gravid females of B. carambolae to different category of infested fruit extracts was probably influenced by the chemicals modification on mango after oviposition. This result suggested that the female B. carambolae do not deposit oviposition deterrent. The phenomenon of deterrence was probably as a result of chemical changes in fruit as a consequence of eggs infestation.

Teknik perbanyakan massal parasitoid Anagrus nilaparvatae (Pang et Wang) (Hymenoptera: Mymaridae) dengan kotak plastik Araz Meilin; Y. Andi Trisyono; Edhi Martono; Damayanti Buchori
Jurnal Entomologi Indonesia Vol 9 No 1 (2012): April
Publisher : Perhimpunan Entomologi Indonesia

Anagrus nilaparvatae (Pang et Wang) is an egg-parasitoid of rice brown planthopper (Nilaparvata lugens Stål). The objective of this study was to develop a mass-rearing technique of A. nilaparvatae. Mass-rearing was done using a plastic box (14 cm × 18.5 cm × 18.5 cm, made of plastic-mica, the upper side equipped with a 10 cm test tube), placed on a tray with rice seedlings containing an estimated of 11,130 eggs of N. lugens. About 355 adult parasitoids were successfully released per box when each box was infested with 50 parasitoids. The number of parasitoids produced were reduced to 164 adults when the infestation was made at 100 parasitoids per box. The emergence of parasitoids started around 07:00 am until 04:00 pm with the highest rate (36.2%) occured at 10:00 am. One plastic box cost only Rp10.000,-; thus the estimate cost to produce one parasitoid using this tecnique was Rp60,- These findings suggest that the plastik box is a simple and inexpensive technique for mass-rearing of A. nilaparvatae.

Pengaruh insektisida deltametrin terhadap perilaku orientasi parasitoid Anagrus nilaparvatae (Pang et Wang) (Hymenoptera: Mymaridae) Araz Meilin; Y. Andi Trisyono; Edhi Martono; Damayanti Buchori
Jurnal Entomologi Indonesia Vol 12 No 3 (2015): November
Publisher : Perhimpunan Entomologi Indonesia

Host searching behaviour by a parasitoid includes orientation to the volatile compounds (odor) relesead by host or host plant. The aim of this study was to determine the changes in the orientation behaviour of Anagrus nilaparvatae (Pang et Wang), the egg parasitoid of rice brown planthoppers (Nilaparvata lugens (Stål)), after being exposed to sublethal concentrations of deltamethrin. The impact of residue on plants to the behaviour of parasitoid was also studied. The sublethal concentrations (LC10 and LC40) used were 0.023 ppm and 2.235 ppm, respectively. Deltamethrin applied to the rice plants were 12.5 ppm and 6.25 ppm, and its effect was observed at 3 hours, 1, 3 and 7 days after application. Orientation behavior was studied using Y-tube olfactometer. Application of deltamethrin at subletal concentration reduced the ability of surviving A. nilaparvatae to detect odors (volatile ompounds) released by the host, N. lugens. The higher the concentration of deltamethrin applied to the parasitoid or to the rice plants, the more prominent effect observed in the changing in the parasitoid behavious, in particular disruption to the parasitoid’s orientation to find their host. As consequences, effects of deltamethrin may lead to reduce the effectiveness of A. nilaparvatae as biological control agents in the field as a result of declining their searching capacity.

PENGOPTIMALAN METODE KROMATOGRAFI CAIR KINERJA TINGGI DALAM ANALISIS SENYAWA DELTAMETHRIN SEBAGAI RESIDU DALAM PRODUK ASAL HEWAN R. Gagak Donny Satria; Bambang Sumiarto; Andi Trisyono; Dwi Wijayanti
Jurnal Kedokteran Hewan Vol 8, No 1 (2014): March
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mendapatkan prosedur atau metode kromatografi cair kinerja tinggi (KCKT) yang valid dan optimal dalam analisis deltamethrin sebagai senyawa yang berpotensi menjadi residu dalam produk hewan. Alat utama yang digunakan dalam penelitian ini adalah satu set KCKT Shimadzu 6.1, dengan kolom C-18 (30° C), panjang gelombang detektor UV-vis 236 nm. Fase gerak yang digunakan dalam penelitian ini adalah asetonitril 80% dalam akuabides yang dialirkan dengan laju 1,25 ml/menit. Hasil penelitian menghasilkan kromatogram yang terlihat menunjukkan peak area yang nyata terpisah dari senyawa lain. Batas deteksi diketahui pada konsentrasi 0,1 μg/ml, sedangkan batas kuantifikasi pada konsentrasi 0,5 μg/ml. Rerata luas area untuk konsentrasi 0,5; 1; 1,5; 2; 5; dan 10 μg/ml masing-masing adalah 18.255,33; 47.142,00; 55.587,00; 64.181,33; 204.269,00; dan 395.918,00 dengan persamaan garis linier y= 39.866x-1.719,5 (R= 0,99). Hasil analisis juga menunjukkan presisi dan akurasi hasil yang baik. Hasil penelitian dapat disimpulkan bahwa metode yang dikembangkan pada penelitian ini mempunyai validitas yang baik dan optimal untuk analisis deltamethrin, yang merupakan senyawa potensial menjadi residu pada produk asal hewan.

Pola sebaran kelompok telur Ostrinia furnacalis Guenée (Lepidoptera: Crambidae) pada lahan jagung da-Lopez, Yosefus F.; Trisyono, Y. Andi; Witjaksono, Witjaksono; Subiadi, Subiadi
Jurnal Entomologi Indonesia Vol 11 No 2 (2014): September
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.11.2.81

Asian corn borer, Ostrinia furnacalis Guenée, is known as an important pest of maize, Zea mays L., in both whorl and reproductive stages. Management decisions based on egg-mass density is useful because decision is made before damage occurs. Observation on O. furnacalis egg-mass distribution in maize-field was carried out in Agricultural Training, Research, and Development Station (ATRD) UGM, Yogyakarta. The number of egg-masses laid on each plant surface in maize-field was sampled in reproductive stage of corn at 52 – 58 days after planting. The spatial dispersion was analyzed using the ratio variance-to-mean (Ïƒ2/Î¼ = IÎ´), Morista's Index (IÎ³), and the negative binomial parameter (Îº-value). The results showed that horizontal and vertical distributions were aggregated distributions (Ïƒ2 > Î¼ or Ïƒ2/Î¼ > 1). In line with the increasing age of plant, the degree of clustering or aggregation likely tended to decline (the Îº-value increased, Morisita index decreased) indicating the possible departure from aggregation to randomness (Poison distribution) due to the heterogeneity of the environment, such as microclimate, preferred parts of the plants, and occurrence of natural enemies. The results concluded that the horizontal and vertical distributions of egg-masses of O. furnacalis on corn in generative phase were clustered with the degree of clustering tended to decrease by the increase of age of corn. These findings provide the bases for further study on the ecology and biology of O. furnacalis for management decision-making process.

Aras kerusakan ekonomi (AKE) larva Ostrinia furnacalis (Lepidoptera: Crambidae) pada tiga fase pertumbuhan tanaman jagung Subiadi, Subiadi; Trisyono, Y. Andi; Martono, Edhi
Jurnal Entomologi Indonesia Vol 11 No 1 (2014): April
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5994/jei.11.1.19

The Asian corn borer, Ostrinia furnacalis (Guenée) is an important economic pest of corn and is widely distributed in the Asia-Pacific region, including Indonesia. The objective of this research is to investigate the effect of infestation levels of O. furnacalis larvae per plant on corn grain yield reductions, and to determine economic injury levels (EIL) of O. furnacalis larvae at three growth stages (V10, R1, and R2) of corn. The experiment was designed in a split-plot randomized complete block with blocks replicated three times. Differences in grain weight between the uninfested and highest infestation levels (six larvae per plant) V10, R1, and R2 were 59.81; 58.76; and 49.20 g/plant, respectively. For each additional infestation by one larvae per plant at V10, R1, dan R2, there is reduction in grain weight of 4.94%, 4.56% and 3.76% respectively. The calculation of EILs was based on the proportion of yield loss per larvae per plant on three corn growth stages with the assumption that pesticide control reduced 67% of O. furnacalis population. The lowest EIL was 0.31 larvae per plant at V10 when the cost of control reaching Rp.125,000 per ha and crop value of Rp.12,000,000 per ha. The highest EIL of 1.24 larvae per plant was found at R2 with the cost of control of Rp.250,000 per ha and crop value of Rp.8,000,000 per ha.

Preliminary Valuation Activity of Calotropis gigantea L. Extracts against Several Insect Pests Khasanah, Nur; Martono, Edhi; Trisyono, Y. Andi; Wijonarko, Arman
Jurnal Perlindungan Tanaman Indonesia Vol 26, No 2 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.71938

Calotropis gigantea produces secondary metabolites. Some toxicity tests on several pest species had been conducted, yet the most susceptible pest species towards C. gigantea extract is still unknown. This study aims to determine the susceptible insect species and study the behavior of susceptible insects to C. gigantea extract. The test methods used in this research were leaf dipping and spraying upon four insect pest species of different orders (Bactrocera carambolae [Diptera: Tephritidae], Nilapavata lugens [Hemiptera: Delphacidae], Sitophilus zeamais [Coleoptera: Curculionidae], and Plutella xylostella [Lepidoptera: Plutellidae]). The concentration used to determine susceptible insects was 25 g.L-1 and control. Deterrence test of susceptible insects using a choice test and no-choice for 1.5 hours observed every five minutes. Five replication were used for each concentration. Behavioral test of susceptible insects using dietary toxicity test. Five bok choy leaf disc was immersed into solutions for 1 minute and air-dried on a petri dish. Observations were carried out for 12 hours with an observation period of every 1.5 hours. A leaf dipping test for 12 hours with an observation period of every 1.5 hours using the feed method. The concentrations used were 12.5 µgL-1and control. The concentration used was the toxicity test of C. gigantea leaf extract 25, 12.50, 6.25. 3.12, 1.56, 0.78, 0.39, 0.19, 0.09, 0.04 µgL-1. P. xylostella was considered a more susceptible test insect towards C. gigantea leaf extract than B. carambolae, N. lugens, and S. zeamais. C. gigantea leaf extract showed a toxic and feeding deterrent to P. xylostella larvae. The LC50 value of C. gigantea extract against P. xylostella by dipping was 16.9 µg.l-1 and 18.5 µg.l-1 by spray. The components of C. gigantea leaf extract consist of alkaloid, tannin, phenol, flavonoid, saponin, and terpenoid. The result of the research showed that C. gigantea leaf extract has a toxic and deterrent substance against P. xylostella and potential to control P. xylostella.

Co-Authors - Mulyadi - Mulyadi - Muryati - Witjaksono Abdul Aziz Agustina Dwi Wijayanti Ahmad Taufiq Arminudin Ananto Triyogo Araz Meilin Araz Meilin Araz Meilin Arman Wijonarko Arman Wijonarko Arman Wijonarko Aryuwandari, Valentina E. F. Aye Kyawt Kyawt Ei Aziz Purwantoro Azzahra, Zulfa Bambang Sumiarto Bambang Sumiarto Budi Setiadi Daryono DAMAYANTI BUCHORI De Faveri, Stefano Didik Indradewa Donny Satria, R. Gagak Dwi Wijayanti Dwi Wijayanti Dyah Rini Indriyanti Eddy Mahrub Edhi Martono Edhi Martono Edhi Martono Edhi Martono George Andrew Charles Beattie George Andrew Charles Beattie Hanna Kawulur, Hanna Hanna S.I. Kawulur Hanna S.I. Kawulur, Hanna S.I. Harahap, Nafsiyah Agustina Harjanto, Sriyanto Harwanto (Harwanto) Helina, Selvi Hendrayanti, Hadvina Nur Hidayat Soesilohadi I. Indriyati Wibisono Ignatius Julijantono Ika Puji Rahmawati K Untung Kasumbogo Untung Kurnia Pratiwi Melhanah Melhanah Mizu Istianto Mizu lstianto Mofit Eko Poerwanto Muliadi, Ahmad Muliadi, Ahmad Muryati Muryati Nasrullah, Nasrullah Nasrullah, Nasrullah Nemay Anggadewi Ndaong, Nemay Anggadewi Nur Khasanah Palupi Jatuasri Paul Holford Praptana, R. Heru R Heru Praptana R. Gagak Donny Satria R. Gagak Donny Satria R.C. Hidayat Soesilohadi Rachmawati, Jeti Sari, Riya Fatma SEDYO HARTONO Serafina T.S. Rahayu Setyaningrum, Ary V. Siwi Indarti Somowiyarjo, Susamto Sri Suharni Siwi SRI SULANDARI St. Margino Subandi Subejo Subiadi Subiadi Subiadi Subiadi Suciati Hadi Wuryaningsih Sudi Pramono . Sumardi . Sumardi Sumardi Sumardiyono, Y. B. Sumardiyono, Y. B. Sumardiyono, Y. B. Suprihanto, Suprihanto Supriyadi Supriyadi Suputa Suputa Suputa Suputa Suryani, Julia Nanda Susamto Somowiyarjo SUWARNO HADISUSANTO T Yuwono Tanjung, Heru Rezki TRIS HARIS RAMADHAN Triwibowo Yuwono Vijaysegaran, Shanmugam Wagiman, Franciscus Xaverius Wahyono - Wahyono Wahyono Wahyu Listyaningrum Widiarta, I Nyoman Witjaksono . Witjaksono Witjaksono Witjaksono Witjaksono Witjaksono Witjaksono Y B Sumardiyono Yosefus F. da-Lopez Yuantomoputro, Arzaq P. Yuni Ratna

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