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All Journal Jurnal Fitopatologi Indonesia Bioma : Berkala Ilmiah Biologi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Hortikultura JURNAL AGROTEKNOLOGI AGRIVITA, Journal of Agricultural Science Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Penelitian Pertanian Tanaman Pangan Biosaintifika: Journal of Biology & Biology Education Jurnal Entomologi Indonesia Jurnal Kedokteran Hewan Jurnal Ilmu-Ilmu Pertanian
Y. Andi Trisyono
Fakultas Pertanian Universitas Gadjah Mada, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Medicine & Pharmacology Veterinary

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Geranium Aralia, Polyscias guilfoylei (W. Bull) L.H. Bailey), Leaf Extract Toxicity against Melon Fly, Zeugodacus cucurbitae Coquillett Rachmawati, Jeti; Martono, Edhi; Trisyono, Y. Andi
Jurnal Perlindungan Tanaman Indonesia Vol 27, No 1 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.75592

Abstract

Zeugodacus cucurbitae Coquillett is one of the most detrimental pests that mostly attack family Cucurbitaceae. Control over this pest often uses insecticides, and one of the plants with the potentiality to be used as source of insecticide is Polyscias guilfoylei. This research aimed to study the influence of P. guilfoylei leaf extract application upon Z. cucurbitae mortality and to determine the LC50 value. The research was conducted at Pest Invertebrate Plant Pest Science Laboratory, Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada. The toxicity test was done using contact, oral and residue methods upon ethanol and n-hexane extracts with 0; 1.25; 2.50; 5.00; 10.00; and 20.00% concentrations. The research used randomized group design with six replications. The parameters observed were the number of dead melon fly at 72 hours after the treatment and LC50 value of each extract. The data were analyzed using Probit analysis via 1.02 version of Lenora software Polo Plus. The result shows that ethanol and n-hexane extracts of P. guilfoylei leaf using oral and residue methods affected Z. cucurbitae mortality. However, in contact method, ethanol and n-hexane extracts of P. guilfoylei leaf did not affect Z. cucurbitae mortality. LC50 values of ethanol extract using oral and residual methods were 6.168% and  3.658%, respectively. LC50 values of n-hexane extract using oral and residual method reached 5.311% and 6.607%, respectively. This research shows that ethanol and n-hexane extracts of P. guilfoylei leaf contain secondary metabolites that are toxic for melon fly Z. cucurbitae. 
Bioassay Method Development to Test Sitophilus oryzae Sensitivity against Phosphine Harahap, Nafsiyah Agustina; Witjaksono, Witjaksono; Martono, Edhi; Trisyono, Y. Andi
Jurnal Perlindungan Tanaman Indonesia Vol 27, No 1 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.75904

Abstract

Sitophilus oryzae is a cereal product pest found in warehouses. The presence of this pest negatively impacts the quality and quantity of stored rice. The common method used to control warehouse pests is fumigation. However, frequent use of insecticides will trigger resistance within target pest populations. This study aimed to develop a bioassay method based on the Food Agriculture Organization (FAO) protocols and modify bioassay protocols to determine S. oryzae susceptibility of populations collected from traditional markets across Yogyakarta. Field populations were collected from eight traditional markets in Yogyakarta (Lempuyangan Market [Kapanewon Danurejan], Kranggan Market [Kapanewon Jetis], Caturtunggal Market [Kapanewon Depok], Godean Market [Kapanewon Godean], Imogiri Market [Kapanewon Imogiri], Pleret Market [Kapanewon Pleret], Kasihan Market [Kapanewon Lendah], and Brosot Market [Kapanewon Galur]). The reference population was obtained from the Southeast Asian Regional Centre for Tropical Biology(SEAMEO BIOTROP). The bioassay was carried out following FAO protocols by using a glass jar fumigation chamber (volume 2 L) which was a modification of the desiccator of FAO recommended method number 16. This test used the lowest testable dose for this method: 0.01 × 10-2 mg/2L or 0.5 × 10-2 µg/L tested on 100 imagoes which were divided into five replications and obtained 100% mortality on the second day (48 hours). Results showed that the FAO fumigation chamber method could not be used in the test, so modifications were carried out to determine tested doses by changing the volume of the fumigation container. The test used three container volumes, including 20, 60, and 80 L. Result from 20 L container showed 82-100% mortality, 60 L containers showed 69-100% mortality, and 80 L containers showed 24-79% mortality. Results from 80 L container was suitable for the bioassay because it was able to test the lowest dose on reference insects. Eight doses ranging from 0.125 to 1.625 × 10-2 µg/L, and an untreated control were used to determine the LD50 of phosphine on each population. Insects were fumigated for 48 hours and then removed to observe mortality. The LD50 of the reference population was 0.27 × 10-2 µg/L while 0.29-0.54 × 10-2 µg/L for field populations. These findings indicate that S. oryzae populations collected from traditional markets in Yogyakarta were still susceptible to phosphine.
Susceptibility of Spodoptera frugiperda J. E. Smith (Lepidoptera: Noctuidae) Collected from Central Java Province to Emamectin Benzoate, Chlorantraniliprole, and Spinetoram Suryani, Julia Nanda; Trisyono, Y. Andi; Martono, Edhi
Jurnal Perlindungan Tanaman Indonesia Vol 26, No 2 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.76195

Abstract

Spodoptera frugiperda J.E. Smith (Lepidoptera: Noctuidae) is a new pest on maize that has successfully spread to various regions in Indonesia. This pest can cause severe damage to the affected plants. Most farmers have relied on synthetic insecticides for control. This research was conducted to determine susceptibility level of S. frugiperda larvae to emamectin benzoate, chlorantraniliprole, and spinetoram insecticides by using the diet-dipping method. There is no registered insecticide to control S. frugiperda, so the three types of insecticides used were based on the recommendation of the Ministry of Agriculture of the Republic of Indonesia. Five field-collected population from Central Java and a laboratory populations of S. frugiperda were used to develop a baseline susceptibility for each insecticide. First instar larvae of S. frugiperda were placed in vials containing artificial diet treated with insecticides and an untreated control (treated with water only), observed for mortality on the seventh day and data was analyzed using probit. S. frugiperda from Tegowanu was the most susceptible to emamectin benzoate (LC50 = 0.11 mg/L), while the least susceptible population was from Wedi (LC50 = 0.39 mg/L) with the resistance ratio (RR) values of 0.45-folds and 1.60-folds compared to the laboratory population, respectively. S. frugiperda from Ngombol was the most susceptible population to chlorantraniliprole (LC50 = 12.63 mg/L), while the least susceptible population was from Tegowanu (LC50 = 30.29 mg/L) with RR values of 0.90- and 2.15-fold compared to the laboratory population, respectively. Meanwhile, S. frugiperda from Jogonalan was the most susceptible to spinetoram (LC50 = 2.75 mg/L), while the population from Wedi was the least susceptible (LC50 = 5.94 mg/L) with the RR values of 0.52- and 1.12-fold compared to the laboratory population. Field-collected S. frugiperda populations were still susceptible to the three tested insecticides tested and emamectin benzoate being the most toxic compared to spinetoram and chlorantraniliprole.
Resistance of Spodoptera exigua Population from Nganjuk against Methomyl, Chlorfenapyr, and Emamectin Benzoate Aziz, Abdul; Martono, Edhi; Indarti, Siwi; Trisyono, Y. Andi
Jurnal Perlindungan Tanaman Indonesia Vol 27, No 2 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.83646

Abstract

Spodoptera exigua Hubner (Lepidoptera: Noctuidae) is the main pest of shallot causing significant economic losses. The continuous use of insecticides has resulted S. exigua to develop resistant against them. This study aims to determine the level of S. exigua resistance of Nganjuk and Bantul populations against methomyl, chlorfenapyr, and emamectin benzoate and temporal resistance of S. exigua of Nganjuk population to methomyl. Nganjuk population were sampled in June, September, and December. First instar larvae from the first generation were used tested using the leaf-dipping method. Temporal resistance test was carried out using the same concentrations. The resistance ratios of Nganjuk population to the insecticides methomyl, chlorfenapyr, and emamectin benzoate were 58.8; 8.8; and 2.5 fold respectively. The LC50 values of S. exigua of Nganjuk population collected in June, September, and December were 1127.44; 50.62; and 366.76 mg [AI] liter-1, respectively. The results of this study indicated that the S. exigua of Nganjuk population was highly resistant to methomyl and its level has changed over time. Resistance management by rotating the type of insecticide is not sufficient but should also consider its change of resistance pattern over time.
Survey of Pesticide Use between Mango Farmers Practicing Area-Wide Integrated Pest Management and Conventional Farming in West Java, Indonesia Sari, Riya Fatma; Subejo, Subejo; Suputa, Suputa; Vijaysegaran, Shanmugam; De Faveri, Stefano; Trisyono, Y. Andi
Jurnal Perlindungan Tanaman Indonesia Vol 28, No 1 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.86684

Abstract

Mango farmers in Indonesia rely heavily on pesticides to control a variety of pests and diseases, with tephritid fruit flies being a major pest of the crop. To reduce pesticide use, Area-Wide Integrated Pest Management (AW-IPM) to control fruit flies was trialed and introduced in 2015 and has now been successfully implemented in several districts in West Java, Indonesia. This study surveyed pesticide use in mango farms adopting AW-IPM and those practicing conventional pest control methods. Purposive and Snowball sampling methods were used to select and interview 83 mango farmers to assess farmers knowledge and practices of pesticide use between the two groups. The farmers surveyed consisted of those that have adopted AW-IPM of fruit flies and conventional farmers, meaning those that did not adopt AW-IPM. The results of the survey revealed that compared to conventional farmers, the AW-IPM farmers had a high level of knowledge of pesticides and most of them were aware of pesticide-related environmental risks and adopted better and safer practices. Overall, storage of pesticides within living areas was reported by 10% of respondents, and most respondents (75%) adopted unsafe practices when disposing of used pesticide containers, such as selling them to plastic waste collectors. Half of all the respondents reported and experienced at least one symptom of a pesticide-related health problems. This study contributes to a better understanding of potential pesticide exposure and risks to mango farmers in Indonesia. A pesticide management training program for the mango farming community is badly needed.
An Update on the Fall Armyworm: Severity of Maize Damage and Susceptibility to Emamectine Benzoate and Chlorantraniliprole Trisyono, Y. Andi; Hendrayanti, Hadvina Nur; Yuantomoputro, Arzaq P.; Setyaningrum, Ary V.; Harjanto, Sriyanto; Aryuwandari, Valentina E. F.
Jurnal Perlindungan Tanaman Indonesia Vol 28, No 2 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.95262

Abstract

The damage area due to fall armyworm (FAW, Spodoptera frugiperda) in Indonesia decreased during the last three years (2021-2023) after the first outbreak in 2019. This insect continues to pose some risk for maize production with most reports documented the damage during the vegetative maize stage showing leave defoliation. This article provides an update on the high field population and the new type of damages caused by FAW by boring the stalks and feeding the cobs observed in the District of Grobogan, Central Java. The populations of egg masses, adults, and larvae were 0.4, 0.17, and 0.37 per plant of 27-day-old, respectively. The reproductive maize was heavily damaged by FAW with leave damage score of 9, 0.33 holes per stalk, and 75% of cobs damaged. The FAW larvae produced from the collected egg masses were still susceptible to emamectin benzoate and chlorantraniliprole. These findings prove that FAW can cause very serious damage leading to almost total yield loss. As maize becomes more important in Indonesia, proper management in compliance with the Integrated Pest Management (IPM) principles is mandatory to keep the population low and prevent large-scale outbreaks.
Population dynamic and pheromone use for early monitoring of Spodoptera exigua Hübner (Lepidoptera: Noctuidae) in Indonesia Witjaksono, Witjaksono; Trisyono, Y. Andi; Tanjung, Heru Rezki; Azzahra, Zulfa
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 24 No. 1 (2024): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12491-97

Abstract

Beet armyworm (Spodoptera exigua) poses a challenge for shallot production because it can exist in fields throughout the year. This study aims to observe the dynamic population during shallot off and on-seasons. Additionally, this study aims to determine the correlation between S. exigua moth captures and damage intensity. The research was conducted from November to May in Kretek Village, Parangtritis District, Bantul Regency, Special Region of Yogyakarta Province, Indonesia. Population dynamic observations of S. exigua during the off-season and on-season were conducted in a 100 x 100 m plot with ten pheromone traps installed. The early monitoring study consisted of two treatments: installed pheromone and control (without the installation of pheromones). The plots were separated by approximately 500 m and were approximately 3500 m2 in size. The results showed that S. exigua exists and can survive during shallot off-season and on-season planting periods. The number of male S. exigua moths captured during the on-season was higher compared to the off-season. The fluctuation of rainfall and the number of S. exigua during the off and on-seasons were also observed, showing that rainfall significantly affected the number of S. exigua moths captured. However, the relationship between rainfall and S. exigua population needs further study. Strong positive correlations between population and damage intensity in pheromone-treated fields between three to seven days after observation (DAO) demonstrated that pheromone traps can be used as a monitoring tool for S. exigua. The close correlation between the S. exigua captured and the intensity of the damage that will occur can be prevented by controlling them by farmers.
PERANAN FAKTOR SUMBER DAYA MANUSIA DALAM MENJAGA KEAMANAN PRODUK PETERNAKAN DARI RESIDU CEMARAN BAHAN KIMIA Donny Satria, R. Gagak; Trisyono, Andi; Ndaong, Nemay Anggadewi
Jurnal Ilmu-Ilmu Pertanian Vol. 21 No. 1 (2015): Juli
Publisher : Politeknik Pembangunan Pertanian Yogyakarta-Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

In the livestock industry, human resource management would drive the success of production, particularly in an effort to meet the needs of animal protein for the community. At present, the problem of exposure of materials or chemicals in poultry products is increasingly becoming a concern. This study was conducted to determine the association of the human resource with the incidence of chemical residues (for example in this research was deltamethrin) in especially broiler meat. The study was conducted in Sleman, Yogyakarta. Multiple stages sampling method was used to collect 74 samples. Residue analysis performed on 512 specimens that carried out in the Laboratory of Pharmacology of the Faculty of Veterinary Medicine UGM. Survey by interviews and questionnaires conducted to determine the human resource management that applied to the farm. Information from the questionnaire was education level, number of workers, and employment status of workers. Descriptive statistical analysis was used to determine the prevalence of residue and overview of the situation of human resource management on the farm. Logistic regression analysis and the calculation of odds ratios were used to determine the association of predictor factors of the probability of the deltamethrin residues in broiler meat. Linear regression analysis was used to determine the association of factors to predict levels of deltamethrin residues. In conclusion, human resource was important factor in securing animal product, in particular from exposure to chemicals and contaminants. In food safety paradigm, the management should pay attention to the competence of human resource and provide proper salary. Efforts to increase competence through training and continuing education is very important.
METODE ALTERNATIF ANALISIS INSEKTISIDA PERTANIAN DELTAMETHRIN MENGGUNAKAN KROMATOGRAFI CAIR KINERJA TINGGI PENGGUNAAN KOLOM C-18, FASE GERAK ASETONITRIL 80% DALAM AKUABIDES, LAJU ALIR FASE GERAK 1,6 ML/MENIT, DAN PANJANG GELOMBANG DETEKTOR UV-VIS 190 NM ( Donny Satria, R. Gagak; Sumiarto, Bambang; Trisyono, Andi; Wijayanti, Agustina Dwi
Jurnal Ilmu-Ilmu Pertanian Vol. 17 No. 1 (2013): Juli
Publisher : Politeknik Pembangunan Pertanian Yogyakarta-Magelang

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Deltamethrin is a common pesticide used in plants and animals. Research about this pesticide has been done with various methods. The purpose of this research was to validate the alternative test method of deltamethrin using High Performance Liquid Chromatography (HPLC). This research used pure deltamethrin. The tool in this research was HPLC Shimadzu 6.1 with 80% acetonitrile in aquabides for mobile phase, flow rate 1.6 ml/min and uv-vis detector wavelength 190 nm. The data were analyzed by calculated the average, standard deviation, and relative standard deviation. The results showed the average area for each concentration of 0.1 ug/ml, 0.25 ug/ml, 0.3 ug/ml, 0.5 ug/ml, 0.75 ug/ml, 1 ug/ml,2 ug/ml, and 5 ug/ml, were 52806.33, 119070.3, 137758.3, 220292, 339677.3, 449448.7, 856049.3, and 2,177,558, with a linear equation y = 43283x + 8455, R = 0,999. It could be concluded that the method is valid based on precision, acuration, specificity, limit of detection, limit of quantification, and linearity.
PENGOPTIMALAN METODE KROMATOGRAFI CAIR KINERJA TINGGI DALAM ANALISIS SENYAWA DELTAMETHRIN SEBAGAI RESIDU DALAM PRODUK ASAL HEWAN Satria, R. Gagak Donny; Sumiarto, Bambang; Trisyono, Andi; Wijayanti, Dwi
Jurnal Kedokteran Hewan Vol 8, No 1 (2014): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v8i1.1264

Abstract

Penelitian ini bertujuan mendapatkan prosedur atau metode kromatografi cair kinerja tinggi (KCKT) yang valid dan optimal dalam analisis deltamethrin sebagai senyawa yang berpotensi menjadi residu dalam produk hewan. Alat utama yang digunakan dalam penelitian ini adalah satu set KCKT Shimadzu 6.1, dengan kolom C-18 (30 C), panjang gelombang detektor UV-vis 236 nm. Fase gerak yang digunakan dalam penelitian ini adalah asetonitril 80% dalam akuabides yang dialirkan dengan laju 1,25 ml/menit. Hasil penelitian menghasilkan kromatogram yang terlihat menunjukkan peak area yang nyata terpisah dari senyawa lain. Batas deteksi diketahui pada konsentrasi 0,1 g/ml, sedangkan batas kuantifikasi pada konsentrasi 0,5 g/ml. Rerata luas area untuk konsentrasi 0,5; 1; 1,5; 2; 5; dan 10 g/ml masing-masing adalah 18.255,33; 47.142,00; 55.587,00; 64.181,33; 204.269,00; dan 395.918,00 dengan persamaan garis linier y= 39.866x-1.719,5 (R= 0,99). Hasil analisis juga menunjukkan presisi dan akurasi hasil yang baik. Hasil penelitian dapat disimpulkan bahwa metode yang dikembangkan pada penelitian ini mempunyai validitas yang baik dan optimal untuk analisis deltamethrin, yang merupakan senyawa potensial menjadi residu pada produk asal hewan.
Co-Authors - Mulyadi - Mulyadi - Muryati - Witjaksono Abdul Aziz Agustina Dwi Wijayanti Ahmad Taufiq Arminudin Ananto Triyogo Araz Meilin Araz Meilin Araz Meilin Arman Wijonarko Arman Wijonarko Arman Wijonarko Aryuwandari, Valentina E. F. Aye Kyawt Kyawt Ei Aziz Purwantoro Azzahra, Zulfa Bambang Sumiarto Bambang Sumiarto Budi Setiadi Daryono DAMAYANTI BUCHORI De Faveri, Stefano Didik Indradewa Donny Satria, R. Gagak Dwi Wijayanti Dwi Wijayanti Dyah Rini Indriyanti Eddy Mahrub Edhi Martono Edhi Martono Edhi Martono Edhi Martono George Andrew Charles Beattie George Andrew Charles Beattie Hanna Kawulur, Hanna Hanna S.I. Kawulur Hanna S.I. Kawulur, Hanna S.I. Harahap, Nafsiyah Agustina Harjanto, Sriyanto Harwanto (Harwanto) Helina, Selvi Hendrayanti, Hadvina Nur Hidayat Soesilohadi I. Indriyati Wibisono Ignatius Julijantono Ika Puji Rahmawati K Untung Kasumbogo Untung Kurnia Pratiwi Melhanah Melhanah Mizu Istianto Mizu lstianto Mofit Eko Poerwanto Muliadi, Ahmad Muliadi, Ahmad Muryati Muryati Nasrullah, Nasrullah Nasrullah, Nasrullah Nemay Anggadewi Ndaong, Nemay Anggadewi Nur Khasanah Palupi Jatuasri Paul Holford Praptana, R. Heru R Heru Praptana R. Gagak Donny Satria R. Gagak Donny Satria R.C. Hidayat Soesilohadi Rachmawati, Jeti Sari, Riya Fatma SEDYO HARTONO Serafina T.S. Rahayu Setyaningrum, Ary V. Siwi Indarti Somowiyarjo, Susamto Sri Suharni Siwi SRI SULANDARI St. Margino Subandi Subejo Subiadi Subiadi Subiadi Subiadi Suciati Hadi Wuryaningsih Sudi Pramono . Sumardi . Sumardi Sumardi Sumardiyono, Y. B. Sumardiyono, Y. B. Sumardiyono, Y. B. Suprihanto, Suprihanto Supriyadi Supriyadi Suputa Suputa Suputa Suputa Suryani, Julia Nanda Susamto Somowiyarjo SUWARNO HADISUSANTO T Yuwono Tanjung, Heru Rezki TRIS HARIS RAMADHAN Triwibowo Yuwono Vijaysegaran, Shanmugam Wagiman, Franciscus Xaverius Wahyono - Wahyono Wahyono Wahyu Listyaningrum Widiarta, I Nyoman Witjaksono . Witjaksono Witjaksono Witjaksono Witjaksono Witjaksono Witjaksono Y B Sumardiyono Yosefus F. da-Lopez Yuantomoputro, Arzaq P. Yuni Ratna