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All Journal Jurnal Akuakultur Indonesia AQUATIC SCIENCE & MANAGEMENT e-Journal BUDIDAYA PERAIRAN JURNAL ILMIAH PLATAX JURNAL PERIKANAN DAN KELAUTAN TROPIS Insan Cita Jurnal Pengabdian Kepada Masyarakat
Henky Manoppo
Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Environmental Science Public Health Social Sciences

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Peningkatan Respon Kebal Non-spesifik dan Pertumbuhan Ikan Nila (Oreochromis niloticus) Melalui Pemberian Jahe, Zingiber officinale Payung, Clara Nunia; Manoppo, Henky
e-Journal BUDIDAYA PERAIRAN Vol 3, No 1 (2015)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.3.1.2015.6925

Abstract

The purpose of this research was to evaluate the effect ginger supplemented in food on nonspecific immune response of nile tilapia.  Juveniles with an average of weight of 27.31 g were obtained from Fish Culture and Development Board  (BP3I) Tateli and then transported to Faculty of Fisheries and Marine Science.  Fish were then cultured in 15 aquaria with a density of 15 fish per aquarium. Each aquarium was equipped with an aerator and used water recirculation system to keep the water quality still in good condition. After adaption for one weeks, fish was fed pellet supplemented with  ginger powder as treatments at five different doses including A=0, B=2,5, C=5, D=7,5, and  E=10 g/kg, each with three replications.  Fish was fed as long as four weeks at 3%/bw/d, twice daily at 08.00 am and 17.00 pm.  Data consisting of total leucocyte count and phagocytosis activity of phagocyte cells were collected at two weeks interval. Research results showed that supplementation of ginger powder into fish pellet  had significant effect on the increase of nonspecific immune response. The highest total leucocyte count and phagocytosis activity of phagocyte cells was observed on fish fed pellet supplemented with 7.5 g/kg of food and significantly different as compared to those of control fish.  As conclusion, supplementation of ginger powder into fish pellet could increase nonspecific immune response on nile tilapia. Keywords: Ginger, nonspecific immune response, total leucocytes count, phagocytosis activity, nile tilapia
Respon kebal nonspesifik ikan mas yang diberi imunostimulan ragi roti secara oral Yanuar, Aditya P.; Manoppo, Henky
e-Journal BUDIDAYA PERAIRAN Vol 5, No 2 (2017)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.5.2.2017.16547

Abstract

The effect of baker’s yeast as immunostimulant on innate immunity of carp (Cyprinus carpio L) had been investigated.  Fish was gathered from Freshwater Aquaculture Board (BBAT) Tatelu, Province of North Sulawesi.  The fish were acclimatized for a week in 15 aquariua with a density of 25 fish/aquarium. After acclimatization the fish were fed pellet supplemented with baker’s yeast as immunostimulant at 0, 5, 10, 15 and 20 g / kg of feed for four weeks as much as 5%/body weight of fish/day with the frequency of feeding twice a day at 08.00 am and 16.00 pm . This study used Completely Randomized Design (RAL) with five treatments and each treatment had three replications. Immune parameters consisting of total leucocytes and phagocytosis activity were measured in the last week. After  four weeks of feeding, the total leukocytes from the carp fed with the addition of baker’s yeast 10 g / kg of feed were significantly increased compared with the fish not being fed with the addition of bread yeast (p <0.01). Phagocytic activity of phagocyte cells from carp also increased significantly in fish fed pellet with the addition of 5 g of baker’s yeast / kg of feed (p = 0.01). In conclusion, the addition of baker’s yeast as immunostimulant in the diet at 5-10 g/kg of pellet can increase the nonspecific immune response of carp. Keywords:  baker’s yeast, carp, nonspesific immune response, total leucocytes, phagocytosis activity
Respon imun benih ikan mas, Cyprinus carpio, yang diberi pakan probiotik Lactobacillus sp. dengan konsentrasi berbeda Pangalila, Novelia; Manoppo, Henky; Tumbol, Reiny A.; Lumenta, Cyska; Kreckhoff, Reni L.; Warouw, Veibe
e-Journal BUDIDAYA PERAIRAN Vol 8, No 1 (2020)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.8.1.2020.27492

Abstract

The research aimed to isolate probiotic bacteria from catfish intestine and assign the concentration of probiotic bacteria which effectively increase the activity of phagocytosis and total leukocytes of carp fingerlings. The fish was taken from Balai Pengembangan dan Pembinaan Pembudidayaan Ikan (BP3I) Tateli. Fish (average initial weight of 2.27 g) were acclimated for one week in 15 aquaria with a density of 25 fish/aquarium. One week after acclimatization, fish were fed with food added with probiotics with different concentrations, namely 1x106, 1x107, 1x108, 1x109 cfu/mL for four weeks. The dose of feed was 5% /body weight/day and feeding time was at 08.00 and 17.00. Data collected were phagocytosis and total leukocyte count. The results showed that supplementation of probiotic significantly increased phagocytic activity and total leukocytes (p<0.01). The best phagocytic activity and total leukocytes were achived in fish fed diet added with probiotics 1x108 cfu/mL. It can be concluded that supplementation of  probiotic onto feed can increase phagocytic activity and total leukocytes of carp.
Penggunaan ragi roti (Saccharomyces cereviciae) secara in situ untuk meningkatkan respon kebal non-spesifik ikan nila (Oreochromis niloticus) Rawung, Meilina E; Manoppo, Henky
e-Journal BUDIDAYA PERAIRAN Vol 2, No 2 (2014)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.2.2.2014.4901

Abstract

The objetive of research was to examine the effect of yeast cell Saccharomyces cereviciae on non-specific immune response of nile tilapia, Oreochromis niloticus. Juveniles weighing 31.83 g in average were cultured in five concrete tanks with a density of 30 fish per tank. The fish were fed pellet supplemented with yeast cell at five different doses (0, 10, 20, 30, 40 g/kg pellet) for three consecutive weeks as much as 4%/bb/day, twice daily. At the end of feeding, three fish from each tank were sampled to measure the immune parameters namely total leucocyte count and phagocytosis activity of phagocyte cells. Research results showed that after three weeks of feeding, total leucocyte and phagocytosis activity of fish fed pellet supplemented with yeast cells were significantly different as compared to those of control fish.  The highest total leucocyte and phagocytosis activity were achieved in fish fed pellet supplemented with 10 g of yeast cells/kg pellet.  It was concluded that the use of yeast cells for three weeks could enhance the nonspecific immune response of nile tilapia. Keywords: Saccharomyces cerreviciae, nonspecific immune response, total leucocyte count, phagocytosis activity, nile tilapia, Oreochromis niloticus
Identifikasi Penyakit Bakterial Pada Benih Sidat (Anguilla marmorata) di Balai Budidaya Air Tawar Tatelu Kusen, Kevin Octavian; Tumbol, Reiny A; Manoppo, Henky
e-Journal BUDIDAYA PERAIRAN Vol 3, No 1 (2015)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.3.1.2015.6935

Abstract

This study aimed to identify the bacterial disease in cultured eel elvers (Anguilla marmorata). The samples used for this study were taken from the Tatelu Freshwater Aquaculture Center. The samples taken were elvers (juvenile life stage of A. marmorata)  which showed clinical symptoms of bacterial infection such as bleeding in the chest, abdomen, and the base of the fin, passive movement, weakness, and loss of balance of the body, loss of appetite, some wounds on back fin, chest, and tail, and the body was not slippery/shiny. Three elvers with the size of 4-6 cm, and weigh about 0.2 g were taken as samples. The organ chosen to be targeted for isolation was head kidney. Each sample was isolated twice as replicates and streaked on Triptic Soy Agar (TSA) medium. First replicates were identified in the Tatelu Health Lab of Freshwater Aquaculture, and the second replicates were identified in North Sulawesi Provincial Health Lab. Bacterial identification was done through a series of observations and morphology of bacterial colonies through gram stain, followed by biochemical tests through oxidase test, catalase test, Triple Sugar Iron Agar (TSIA) test, H2S production test, motility test, O/F test, and Simmons Citrate Agar (SCA) test. Water quality parameters that were measured included temperature, disolve oxygen, pH, and ammonia. Based on identification of bacteria in cultured elvers in Tatelu Freshwater Aquaculture Center, the elver samples had been infected with bacterial  Aeromonas hydrophila. Keywords: elver, identification, Aeromonas hydrophila, Tatelu Freshwater Aquaculture Center
Efikasi ekstrak tanaman obat Binahong Anredera cordifolia (Ten) Steenis untuk meningkatkan pertumbuhan ikan Nila Oreochromis niloticus Wantah, Monika M.; Longdong, Sammy N.J.; Kreckhoff, Reni L.; Mantiri, Desy M.H.; Tumbol, Reiny A.; Manoppo, Henky
e-Journal BUDIDAYA PERAIRAN Vol 6, No 2 (2018)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.6.2.2018.20502

Abstract

The aim of this research was to study the effect of Binahong extract on the growth of Nile tilapia. A number of 225 fish measuring 10-12 cm in length with an average weight of 15 g was obtained from Fishery Development Center and Training, Tateli. The fish were put  in oxygenated plastic bags and transported to Laboratory of Aquaculture Technology. The fish was kept in aquarium equipped with an aerator with a density of 15 fish per aquaria. The test material used was the stem of Binahong plant stem, macerated with alcohol 70% for 3 days. The extract was then filtered with whatman filter paper and extract was concentrated using a Rotary Evaporator. The extract was further diluted to obtain  concentrations of 12.5, 25, 50, and 100 mg / mL Binahong  extract as treatment was given to the fish after mixed first into the pellet. The fish was fed with treatment feed for 30 days. Control feed was not supplemented with binahong extract. Growth data was measured at day 30 after treatment. The results showed that the extract of binahong had significant effect on fish growth (p <0.01) with the best result achieved at concentration of 100 mg/mL. In conclusion, supplementation of  binahong extract into fish feed could improve growth of  fish in which the best growth was obtained in fish fed diet containing 100 mg/mL of binahong extract.Keywords: Medicinal plants, tilapia, Anredera cordifolia, growth, maceration
The use of baker’s yeast (Saccharomyces cereviciae) as immunostimulant to enhance resistance of nile tilapia (Oreochromis niloticus) to Aeromonas hydrophila Manurung, Usy N; Manoppo, Henky; Tumbol, Reiny A
AQUATIC SCIENCE & MANAGEMENT Edisi Khusus 2 (2014): Oktober
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/jasm.0.0.2014.7300

Abstract

The objective of this research was to evaluate the efficacy of Baker’s Yeast S. cereviciae in enhancing the resistance of nile tilapia (Oreochromis niloticus) to A. hydrophila. As many as 250 fish with an average weight of 28.78±2.44 g were obtained from Fish Culture Development and Training Center of Tateli. After acclimatization, the fish were fed pellet supplemented with Baker’s Yeast as treatments at five different doses, A=0 gr/kg feed, B=5 gr/kg feed, C=10 gr/kg feed, D=15 gr/kg feed, and E=20 gr/kg feed each of which was with three replications.  They were fed for four weeks at 5%/BW/day, twice a day at 08.00 and 16.00, respectively. After feeding period, the fish were challenged intraperitoneally with A. hydrophila.  Before injection, a pathogenicity test of bacteria A. hydrophila was conducted for LD50. Challenged test was carried out by injecting fish with 0.2 ml of bacterial suspension containing 5 x 106 cfu/ml. The fish resistance was observed for 14 days. Dead fish were taken out and bacterial isolation was performed to confirm the cause of the dead.  Results showed that supplementation of Baker’s Yeast into fish pellet had significant effect  on the fish resistance (p=0.00).  The highest resistance (66.6%) was recorded in fish fed with pellet supplement of 5 g Baker’s Yeast per kg of pellet while control fish was only 50%.  As conclusion, supplementation of Baker,s Yeast into fish pellet could enhance resistance of fish to the pathogen. Penelitian ini bertujuan untuk menguji efikasi ragi roti S. cereviciae dalam meningkatkan resistensi ikan nila (Oreochromis niloticus)terhadap bakteri patogen A. Hydrophila. Sebanyak 250 ekor ikan nila dengan berat awal rata-rata 28,78±2,44 g yang diambil dari Balai Pengembangan dan Pembinaan Pembudidayaan Ikan (BP3I) Tateli. Setelah aklimatisasi, ikan diberi ragi roti sebagai perlakuan dengan lima dosis berbeda dan masing-masing perlakuan memiliki tiga ulangan.Perlakuan ragi roti yang digunakan adalah A=0 gr/kg pakan, B=5 gr/kg pakan, C=10 gr/kg pakan, D=15 gr/kg pakan, E=20 gr/kg pakan. Lama pemberian pakan perlakuan empat minggu dengan dosis 5%/bb/hari dan diberikan 2 kali sehari yaitu Pukul 08.00 dan Pukul 16.00. Setelah diberikan ragi roti selama empat minggu ikan diuji tantang dengan bakteri A. Hidrophyla secara Intraperopeneal (ip). Sebelum penyuntikan, dilakukan uji patogenitas bakteri yang memberikan tingkat kematian 50% (LD50). Uji tantang dilakukan dengan cara menyuntikkan 0,2 ml suspensi bakteri pada kepadatan 5 x 106 cfu/ml (sesuai hasil uji patogenitas) pada rongga tubuh ikan. Pengamatan resistensi ikan akan dilakukan setiap hari selama 14 hari. Ikan mati dikeluarkan dan dilakukan isolasi bakteri untuk mengkonfirmasi penyebab kematian ikan. Hasil penelitian mendapatkan bahwa pemberian ragi roti berpengaruh sangat nyata (P=0,00) pada resistensi ikan terhadap bakteri A. Hydrophila. Resistensi tertinggi dicapai pada ikan yang diberi perlakuan B (5 g/kg pakan) dengan tingkat resistensi mencapai 66,6%. Sedangkan ikan yang tidak diberi perlakuan ragi roti (control) memiliki resistensi 50 %. Sebagai kesimpulan penambahan ragi roti dalam pakan dapat meningkakan resistensi ikan terhadap infeksi pathogen.
DETERMINASI MOLEKULER KOI HERPES VIRUS (KHV) YANG DIISOLASI DARI IKAN KOI (Cyprinus carpio koi) Saselah, Jetti Treslah; Tumbol, Reiny A; Manoppo, Henky
JURNAL PERIKANAN DAN KELAUTAN TROPIS Vol 8, No 2 (2012)
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (263.525 KB) | DOI: 10.35800/jpkt.8.2.2012.408

Abstract

Penelitian ini bertujuan untuk mendeterminasi keberadaan Koi Herpes Virus (KHV) pada ikan koi (Cyprinus carpio koi). Sampel ikan diambil dari Kabupaten Kepulauan Sangihe. Penelitian di lakukan di Laboratorium Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Sam Ratulangi dengan menggunakan metode Polymerase Chain Reaction (PCR). Hasil pemeriksaan PCR terhadap sampel ikan koi mengindikasikan bahwa sampel ikan koi telah terinfeksi oleh virus KHV, yang ditandai dengan munculnya pita DNA pada hasil visualisasi elektroforesis agarosa. Hasil pemeriksaan pada morfologi ternyata sampel ikan yang digunakan menunjukkan gejala-gejala klinis terserang KHV seperti mata pucat, insang berwarna pucat serta produksi lendir yang berlebihan. Beberapa sampel lainnya walaupun secara morfologi belum menunjukan gejala-gejala klinis tetapi melalui pemeriksaan PCR telah terindikasikan terinfeksi KHV. Kata kunci: KHV, PCR, ikan koi   The purpose of this study was to determine the presence of Koi Herpes Virus (KHV) in Koi (Cyprinus carpio koi). Fish samples were taken from the Sangihe Archipelago Regency. Laboratory work conducted at the Laboratory of Biology Faculty of Mathematics and Natural Sciences University of Sam Ratulangi using Polymerase Chain Reaction (PCR) method. The samples indicated that the koi fish samples were infected with KHV. It is shown by the appearance of DNA bands on agarose electrophore­sis visualization. The morphological examination of the body indicated clinical symptoms of KHV infection, such as pale eyes, pale gills and excessive mucus production. Several other samples, although without clinical symptoms, have shown to be infected as indicated by PCR test. Keywords: KHV, PCR, Koi.
Penggunaan bakteri probiotik untuk pencegahan infeksi bakteri Streptococcus agalactiae pada ikan Nila, Oreochromis niloticus Umasugi, Aisin; Tumbol, Reiny A.; Kreckhoff, Reni L.; Manoppo, Henky; Pangemanan, Novie P.L.; Ginting, Elvi L.
e-Journal BUDIDAYA PERAIRAN Vol 6, No 2 (2018)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.6.2.2018.20556

Abstract

This study aimed to evaluate the effect of probiotic on growth and survival of tilapia (Oreochromis niloticus) against Streptococcus agalactiae infection. The test materials used were commercial probiotic bacteria and Streptococcus agalactiae. The probiotic bacteria were administered by mixing into the feed with a dose of 0 mL (without probiotics), 10 mL / kg of feed, 15 mL / kg of feed and 20 mL / kg of feed.  Feed was given 3 times a day at 08.00, 12.00 and 16.00 for 21 days. After that, the challenge test was done with Streptococcus  agalactiae bacteria by cohabitation. Bacteria with a density of 107 cells / mL were mixed into the water and allowed for 2 hours. The result showed that B treatment (10 mL / kg of feed) gave the best result with 93,33 percent of  survival rate. Statistical analysis showed that the survival of fish in treatments B, C and D was significantly different from treatment A (P <0.1). However, there was no significant difference between treatments B, C, and D. It was also found that the addition of probiotic in feed did not affect the growth of Nile tilapia (Orechromis niloticus). Keywords : Probiotic bacteria,  nile tilapia,  Streptococcus  agalactiae, growth,  infection
Peningkatan Respon Imun Non Spesik Ikan Mas (Cyprinus carpio L) Melalui Pemberian Bawang Putih (Allium Sativum) Lengka, Kedis; Manoppo, Henky; Kolopita, Magdalena E.F.
e-Journal BUDIDAYA PERAIRAN Vol 1, No 2 (2013)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.1.2.2013.1912

Abstract

ABSTRACT This research aimed to determine the proper dose of white onion (Allium sativum) powder to stimulate the nonspecific immune response and growth of carp (Cyprinus carpio L). Fish (length 8-12 cm; weight 9.37 g in average) were obtained from BBAT Tatelu (Freshwater Aquaculture Board). After acclimatized in 1000-l fibre tank for two weeks, fish were introduced to 15 glass aquaria (80x40x40 cm) equiped with aeration at a density of 15 fish/aquarium. Fish were fed diet supplemented with white onion powder at five different doses (0, 10, 20, 30 and 40 g/kg diet), twice daily at 5%bw. Data collected included total leucocyte count (TLC), phagocytosis activity and growth of fish. Results showed that after four weeks of feeding, supplementation of white onion powder at 30 g/kg diet was effective to enhance nonspecific immune response and growth of carp. Keywords: Allium sativum, immune response, total leucocyte count, phagocytosis activity
Co-Authors Achmad A Bugis Adnan Wantasen Angkouw, Esther D. Ayu Maleko Bella, Sintia S. Clara Nunia Payung Cyska Lumenta Daniel Djokosetiyanto Darus S. Paransa Edwin D Ngangi Enang Harris Erly Y. Kaligis, Erly Y. Gercyana Marentek Ginting, Elvi L. Ginting, Elvie L. Ginting, Elvy Like Grace O. Tambani Hengky J Sinjal Hengky J. Sinjal, Hengky J. Henneke Pangkey Hutagaol, Anggun F. Jetti Treslah Saselah Joppy Mudeng Karisoh, Yuriani S. Kedis Lengka Kemer, Kurnati Kevin Octavian Kusen Kreckhoff, Reni L. Lukas Belseran Maarisit, Ismariani Magdalena E.F Kolopita Magdalena E.F. Kolopita Mantiri, Desy M. H Maweikere, Fabiola C. Meilina E Rawung Mochamad Fatuchri Sukadi Mokoginta, Fatika Sari Mokolensang, Jeffrie F. Monijung, Revol D. Mudeng, Cindy C. Natalie D Rumampuk Nickson J. Kawung, Nickson J. Novitarizky, Intan A. Pangalila, Novelia M.A. Pangemanan, Novie P.L. Pangkey, Hanneke Paulus, James J. H. Ratusmanga, Jihan Reiny A Tumbol Reiny A. Tumbol Remy E. P Mangindaan Sambuaga, Mutiara E. Sammy N.J Longdong Sammy N.J. Longdong Sartje Lantu Simanjuntak, Erika E. L. Sinubu, Wulan V. Sukenda . Suria Darwisito, Suria Suzanne L Undap Takou, Putri D. Umasugi, Aisin Undi, Chintia S. Usy N Manurung Veibe Warouw Wantah, Monika M. Yanuar, Aditya P.