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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology ACTA VETERINARIA INDONESIANA MEDIA KONSERVASI Jurnal Veteriner Jurnal Sain Peternakan Indonesia Jurnal Ilmu Ternak Veteriner Journal of the Indonesian Tropical Animal Agriculture Jurnal Peternakan Indonesia Livestock and Animal Research Animal Production : Indonesian Journal of Animal Production ARSHI Veterinary Letters Logista: Jurnal Ilmiah Pengabdian Kepada Masyarakat Jurnal Ilmu Peternakan Terapan Jurnal Nukleus Peternakan Jurnal Peternakan Lahan Kering JIA (Jurnal Ilmiah Agribisnis) : Jurnal Agribisnis dan Ilmu Sosial Ekonomi Pertanian
Wilmientje Marlene Mesang Nalley
Fakultas Peternakan Universitas Nusa Cendana Kupang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Social Sciences Veterinary

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Journal : Jurnal Ilmu Ternak Veteriner

 1 
Viability of Timor deer stag (Cervus timorensis) spermatozoa extended in tris egg yolk diluent with different sources of carbohydrate and storage at room temperature Mesang-Nalley, W. Marlene; Handarini, R; Purwantara, B
Indonesian Journal of Animal and Veterinary Sciences Vol 12, No 4 (2007)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (60.943 KB) | DOI: 10.14334/jitv.v12i4.499

Abstract

The successful  sperm preservation, influenced by the capability of its extender on the maintenance the sperm quality during storage. The carbohydrate such as glucose and fructose were the common sugar added on the mammalian sperm extender to support their live and motility. The sucrose was the main carbohydrate in  Timor deer stag seminal plasma. The experiment was conducted to evaluate the effect of carbohydrates  in Tris egg yolk (TEY) extender on the motility and viability of stag sperm, stored in room temperature (27-28 oC). The semen was collected using electro ejaculator from five Timor deer stags at hard antler stage, 3-5 years old, body weight of 64-102 kg with normal testes. The semen was than evaluated macro-and microscopically and divided into 3 aliquots.  Each of them was diluted with TEY-glucose (TEYG), TEY-fructose (TEYF) and TEY-Sucrose (TEYS) with the concentration of spermatozoa 100 x 106 ml-1. The extended semen was than stored at room temperature. The sperm motility and viability were evaluated every 3 hours. Result of the experiment showed that the semen volume was 2.06 ± 0.63 ml, pH 7.03±0.13, yellow white until creamy in color and the consistency ranged from normal to thick. The mass movement between ++ to +++ and the sperm motility was 68.67 ± 7.4%. The average of sperm concentration was 842.35 ± 258.14x106 ml-1, the viable sperm was 78.11 ± 3.61%, the sperm abnormality was 7.31 ± 2.98%. The percentages of sperm motility on TEYG (18.00 ± 17.63%) and TEYS (21.83 ± 15.92%) were higher compare to TEYF (4,00 ± 0,00%) extender in 24 hours observation. The percentage of sperm viability showed the same pattern. The sperm viability in TEYG (28.17 ± 20.06) and TEYS (24.00 ± 22.59%) (P<0.05) were significantly higher compare to TEYF (4.00 ± 0.00%).  It is concluded that the deer stag sperm can use the three sugars for their nutrition source. The diluted sperm still can be used  for artificial insemination after 12 hour storage. Key Words: Liquid Semen, Deer, Room Temperature, Carbohydrate
Effect of Priangan ram seminal plasma on viability of Peranakan Etawah buck spermatozoa preserved at 3–5oC Rizal, Muhammad; ., Herdis; Surachman, Maman; Mesang-Nalley, W. Marlene
Indonesian Journal of Animal and Veterinary Sciences Vol 13, No 1 (2008)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (93.075 KB) | DOI: 10.14334/jitv.v13i1.591

Abstract

In processing of buck semen, seminal plasma is a problem because it contains a phospholipase A enzime produced by the Cowper gland. If this enzime interacts with egg yolk, it causes semen coagulation, and consequently death of spermatozoa. The purpose of this research was to examine the effect of Priangan ram seminal plasma on viability of Peranakan Etawah (PE) buck spermatozoa preserved at 3–5oC. Semen was collected using artificial vagina once a week. Fresh semen was divided into three tubes then centrifuged at 3,000 RPM for 30 min. Supernatant of the first tube was mixed again with Pasteur pipette (treatment A or control). Supernatant of the second tube was removed (treatment B or without seminal plasma). Supernatant of the third tube was removed and changed with Priangan ram seminal plasma in the same volume (treatment C). Semen was diluted with Tris extender containing 20% egg yolk and stored in refrigerator at 3–5oC. Quality of diluted-semen including percentages of motile spermatozoa (MS), live spermatozoa (LS), and intact plasma membrane (IPM) was evaluated every day during storage at 3–5oC for three days. Results of this study showed that mean volume, colour, consistency, pH, mass activity, spermatozoa concentration, MS, LS, spermatozoa abnormal, and IPM of PE buck fresh semen, respectively was 0.68 ml, cream, thick, 7, ++/+++, 4,148.57 million cell/ml, 70%, 83.89%, 7.12% and 84%. At day-4 of storage, percentages of MS, LS, and IPM for treatment C (40, 52.2 and 51.6%) was significantly (P<0.05) higher than that of: treatment B (31, 44.8 and 45.2%) and treatment A (11, 15.6 and 14.8%). In conclusion, seminal plasma of Priangan ram could maintain the quality of PE buck semen preserved at 3–5oC for three days, and it prevent semen from coagulation. Key Words: Seminal Plasma, Priangan Ram, Spermatozoa Viability, PE Buck
Viability of Timor deer stag (Cervus timorensis) spermatozoa extended in tris egg yolk diluent with different sources of carbohydrate and storage at room temperature W. Marlene Mesang-Nalley; R Handarini; B Purwantara
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 4 (2007): DECEMBER 2007
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (60.943 KB) | DOI: 10.14334/jitv.v12i4.499

Abstract

The successful  sperm preservation, influenced by the capability of its extender on the maintenance the sperm quality during storage. The carbohydrate such as glucose and fructose were the common sugar added on the mammalian sperm extender to support their live and motility. The sucrose was the main carbohydrate in  Timor deer stag seminal plasma. The experiment was conducted to evaluate the effect of carbohydrates  in Tris egg yolk (TEY) extender on the motility and viability of stag sperm, stored in room temperature (27-28 oC). The semen was collected using electro ejaculator from five Timor deer stags at hard antler stage, 3-5 years old, body weight of 64-102 kg with normal testes. The semen was than evaluated macro-and microscopically and divided into 3 aliquots.  Each of them was diluted with TEY-glucose (TEYG), TEY-fructose (TEYF) and TEY-Sucrose (TEYS) with the concentration of spermatozoa 100 x 106 ml-1. The extended semen was than stored at room temperature. The sperm motility and viability were evaluated every 3 hours. Result of the experiment showed that the semen volume was 2.06 ± 0.63 ml, pH 7.03±0.13, yellow white until creamy in color and the consistency ranged from normal to thick. The mass movement between ++ to +++ and the sperm motility was 68.67 ± 7.4%. The average of sperm concentration was 842.35 ± 258.14x106 ml-1, the viable sperm was 78.11 ± 3.61%, the sperm abnormality was 7.31 ± 2.98%. The percentages of sperm motility on TEYG (18.00 ± 17.63%) and TEYS (21.83 ± 15.92%) were higher compare to TEYF (4,00 ± 0,00%) extender in 24 hours observation. The percentage of sperm viability showed the same pattern. The sperm viability in TEYG (28.17 ± 20.06) and TEYS (24.00 ± 22.59%) (P<0.05) were significantly higher compare to TEYF (4.00 ± 0.00%).  It is concluded that the deer stag sperm can use the three sugars for their nutrition source. The diluted sperm still can be used  for artificial insemination after 12 hour storage. Key Words: Liquid Semen, Deer, Room Temperature, Carbohydrate
Effect of Priangan ram seminal plasma on viability of Peranakan Etawah buck spermatozoa preserved at 3–5oC Muhammad Rizal; Herdis .; Maman Surachman; W. Marlene Mesang-Nalley
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 1 (2008): MARCH 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (93.075 KB) | DOI: 10.14334/jitv.v13i1.591

Abstract

In processing of buck semen, seminal plasma is a problem because it contains a phospholipase A enzime produced by the Cowper gland. If this enzime interacts with egg yolk, it causes semen coagulation, and consequently death of spermatozoa. The purpose of this research was to examine the effect of Priangan ram seminal plasma on viability of Peranakan Etawah (PE) buck spermatozoa preserved at 3–5oC. Semen was collected using artificial vagina once a week. Fresh semen was divided into three tubes then centrifuged at 3,000 RPM for 30 min. Supernatant of the first tube was mixed again with Pasteur pipette (treatment A or control). Supernatant of the second tube was removed (treatment B or without seminal plasma). Supernatant of the third tube was removed and changed with Priangan ram seminal plasma in the same volume (treatment C). Semen was diluted with Tris extender containing 20% egg yolk and stored in refrigerator at 3–5oC. Quality of diluted-semen including percentages of motile spermatozoa (MS), live spermatozoa (LS), and intact plasma membrane (IPM) was evaluated every day during storage at 3–5oC for three days. Results of this study showed that mean volume, colour, consistency, pH, mass activity, spermatozoa concentration, MS, LS, spermatozoa abnormal, and IPM of PE buck fresh semen, respectively was 0.68 ml, cream, thick, 7, ++/+++, 4,148.57 million cell/ml, 70%, 83.89%, 7.12% and 84%. At day-4 of storage, percentages of MS, LS, and IPM for treatment C (40, 52.2 and 51.6%) was significantly (P<0.05) higher than that of: treatment B (31, 44.8 and 45.2%) and treatment A (11, 15.6 and 14.8%). In conclusion, seminal plasma of Priangan ram could maintain the quality of PE buck semen preserved at 3–5oC for three days, and it prevent semen from coagulation. Key Words: Seminal Plasma, Priangan Ram, Spermatozoa Viability, PE Buck
Co-Authors A. Kaka Agus Saputra Aleksander Kaka Alfred Nubatonis Aloysius Marawali Anak Agung Gede Sugianthara Arnoldianus Harbin Asnawati Is, Asnawati Is B Purwantara B Purwantara B. Purwantara Bambang Purwantara Biqshierz V.W Koelima Bire Lawa, Alvrado Burhanuddin * Burhanuddin burhanuddin Demianus Ferdinand Souhoka Fian K. Djita Fikriman, Fikriman Franky M S Telupere G Semiadi G. Semiadi Gaina, Cynthia Dewi GONO SEMIADI Henderian L. L. Belli Henderiana Belli Hendriana L.L. Belli Herdis . Heri Armadianto Heri Armadianto I Made Adi Sudarma Imanuel Benu J A Tamoes Jernih Blegur Johny N. Kihe Juliyanti Stefania Salem Kirenius Uly Kirenius Uly kirenius uly Maman Surachman Maman Surachman Mardemus Misa Maria E.E. Fernandez Maria Epifania Barek Maria F. T. Leyn Maria G. Mega Maria Gordince Owa Maria M. Bria Mariana Nenobais Maritje Aleonor Hilakore Maxs Urias Ebenheizer Sanam Melianus Fafo Michel Johan Matatula MUHAMMAD RIZAL N.G.A Mulyantini S.S Petrus Kune Petrus Kune Petrus Kune R Handarini R I Arifiantini R. Handarini R. Handarini R. Iis Arifiantini R.I. Arifiantini Reni Ratni Dapawole RI Arifiantini, RI Ristika Handarini Satria J.S. Rihileo Selsiana Dongkot Sepriyono Aklili Setiono Setiono Sofia Marlize Soleman Manaze Weiri Sinda T L Yusuf T.L. Yusuf Tagu Dodu Thomas Mata Hine Tree May Thiessen Outang Tuty Laswardi Yusuf Twen O. Dami Dato Ulrikus R. Lole Wirona Adu Yohanis Umbu Daku Waluwanja