Garuda - Garba Rujukan Digital

p-Index From 2021 - 2026

2.042

P-Index

This Author published in this journals

All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Media Veteriner Jurnal Sain Veteriner ANNALES BOGORIENSES Jurnal Medik Veteriner ARSHI Veterinary Letters FITOFARMAKA: Jurnal Ilmiah Farmasi Current Biomedicine Annales Bogorienses

Kusdiantoro Mohamad

Fakultas Kedokteran Hewan Institut Pertanian Bogor

Author-ID : 321312

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

Published : 18 Documents Claim Missing Document

Claim Missing Document

Articles

1 2

Biological Analysis of Leydig Cells-Conditioned Medium To Support Rat Bone Marrow Mesenchymal Stem Cells Differentiation Kaiin, Ekayanti Mulyawati; Prasetyaningtyas, Wahono Esthi; Mohamad, Kusdiantoro; Djuwita, Ita; Yusuf, Tuty Laswardi; Setiadi, Mohamad Agus
ANNALES BOGORIENSES Vol 22, No 1 (2018): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ab.v22i1.328

The developed Leydig cells-conditioned medium (LCM) contains bioactive materials secreted by Leydig cells in vitro.Â LCM was used to evaluate the ability of bone marrow mesenchymal stem cells differentiation. Bone marrow mesenchymal stem cells (1x 106 cell/ml) were cultured in : 1) DMEM supplemented with 10% NBCS as a control (M), 2) M supplemented with 10 ng/ml testosterone; 3) M supplemented with 50%Â LCM ; 4) M supplemented with 50% LCM and 2.5 IU/ml hCG. Bone marrow mesenchymal stem cells that were cultured withÂ LCM has a positive reaction (57.4%) to histochemistry staining 3Î²-HSD and produced 1.87 ng/ml testosterone. Supplementation of hCG to LCMÂ increased the positive number of Leydig cells and testosterone production by 74.6% and 12.33 ng/ml (P<0.05). It can be concluded that Leydig cells-conditioned medium can support differentiation of bone marrow mesenchymal stem cells into Leydig cells.

Analisis Proteomik Cairan Sinovial Sendi Domba: Efektivitas Metode dan Profil Protein Fungsional Mohamad, Kusdiantoro; Rahmaniyah, Wiwit Ridhani; Adnyane, I Ketut Mudite; Fahrudin, Mokhamad; Boediono, Arief
Acta VETERINARIA Indonesiana Vol. 8 No. 2 (2020): Juli 2020
Publisher : IPB University

Penelitian ini bertujuan untuk mengevaluasi metode deplesi dan digesi protein, serta menganalisis proteom cairan sinovial (SF) sendi pada domba sehat menggunakan kromatografi cairâ€“tandem spektrometri massa. Cairan SF dikoleksi dari enam ekor domba garut betina, umur Â±4 tahun, berat 35-40 kg, dan sehat secara klinis. Deplesi protein berkelimpahan tinggi dilakukan dengan metode spin column menggunakan TOP 12 dan metode proteospin, sementara digesi protein dengan tiga metode, yaitu digesi in solution, in gel, dan in-solution + filter-aided sample preparation (FASP). Metode yang terbaik kemudian dilanjutkan dengan fraksinasi peptida menggunakan ultra performance liquid chromatography. Metode deplesi proteospin dengan digesi protein in-solution + FASP merupakan metode terbaik berdasarkan nilai coverage dan sequest HT. Hasil analisis proteomik terkarakterisasi 52 protein pada database spesies domba. Anotasi gen ontologi menggunakan DAVID analysis menunjukkan bahwa protein-protein SF tersebut merupakan komponen sel terutama sebagai eksosom ekstraseluler, fungsi molekuler sebagai aktivitas inhibitor endopeptidase tipe-serin dan pengikat ion kalsium; serta proses biologis sebagai angiogenesis dan koagulasi darah atau pembentukan fibrin. Analisis KEGG pathway menunjukkan protein SF berperan utama pada jalur kaskade koagulasi dan komplemen.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.18.4.151

Heterotopic grafting sites can be useful in producing oocytes for in vitro Fertilization, therefore, maximising the oocyte yield from the graft by gonadotrophin stimulation would be advantageous. The aim of this study was to investigate the number and quality of oocytes collected from heterotopic autografted ovary after Pregnant Mare Serum Gonadothropin (PMSG) induction. Graft recipients were treated either with or without PMSG stimulation 48 hours prior to graft collection. Ovarian tissue from four weeks old mice (DDY strain) were autotransplanted under the kidney capsule of the same ovariectomized mice and the oocytes were collected 21 days after autotransplantation. The results showed that the average number of oocytes collected from autografted ovaries without PMSG induction were 9.0. + 2.8 not significantly different with those received PMSG induction, 10.9 + 5.1. The percentage of matured and fertilized oocytes and the developed embryos from the autografted ovaries without PMSG induction were 52.4, 33.4, and 26.0%, respectively not significantly different with those received PMSG induction, 53.2, 35.1, and 29.9%, respectively. The number of oocytes and the capacity to matured, fertilized and developed were significantly lower (P < 0.05) compared to the superovulated nongrafted (control) ovaries. In conclusion, PMSG induction on the graft recipients did not significantly increase oocytes yield from grafted heterotopic ovaries. The number and quality of oocytes produced from the autografted ovaries were lower than the superovulated nongrafted ovaries, but still can be used for in vitro embryo production after sequential in vitro maturation and fertilization.

Allotransplantasi Testis Mencit Muda sebagai Upaya Preservasi Gonad In Vivo Wahono Esthi Prasetyaningtyas; Kusdiantoro Mohamad; Mokhamad Fahrudin; Ita Djuwita; Srihadi Agungpriyono
Jurnal Ilmu Pertanian Indonesia Vol. 12 No. 1 (2007): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (166.442 KB)

Cancer disease are not detected only at adult but also at young age. One therapy for cancer diseases is chemotherapy and radiation, that give a side effect of infertility in the gonad, therefore, it is necessary to preserve the gonad. Sperm collection from adult is easy but not from the young patients.Keyword: transpalantation, testis, mencit muda, spermatogenesis

Pemaparan Gelombang Elektromagnetik Telepon Genggam pada Mencit (Mus musculus albinus) Periode Awal Kebuntingan Vincentia Maria; Kusdiantoro Mohamad; Arief Boediono
Acta VETERINARIA Indonesiana Vol. 2 No. 1 (2014): Januari 2014
Publisher : IPB University

Peningkatan penggunaan telepon genggam diiringi dengan peningkatan kepedulian masyarakatterhadap bidang kesehatan dan keamanan terkait emisi gelombang elektromagnetik yang berasaldari telepon genggam. Penelitian ini bertujuan mengetahui tingkat keamanan paparan gelombangelektromagnetik telepon genggam melalui pengamatan terhadap jumlah implantasi dan anak lahirdengan menggunakan mencit sebagai hewan model. Dua puluh empat ekor mencit betina disinkronisasidan dikawinkan dengan mencit jantan dengan perbandingan jantan dan betina 1:1 (single mating).Pemaparan dilakukan dengan gelombang berfrekuensi 900 MHz selama 7 hari pertama setelah mencitkawin. Mencit betina dibagi ke dalam empat kelompok berdasarkan lamanya waktu paparan. Waktupaparan adalah satu, dua, dan empat kali per hari dengan masing-masing lama paparan 15 menit untukkelompok pertama, kedua, dan ketiga, sementara kelompok keempat sebagai kelompok kontrol tidakdiberikan paparan. Hasil penelitian menunjukkan tidak adanya pengaruh nyata lama paparan gelombangelektromagnetik telepon genggam terhadap jumlah implantasi dan anak lahir dari induk yang terpapar(P>0,05). Untuk seluruh kelompok, jumlah implantasi berkisar antara 8,66 sampai dengan 10,00 danjumlah anak lahir berkisar antara 10,00 sampai dengan 12,33; tidak berbeda nyata dengan nilai padakelompok kontrol. Berdasarkan hasil penelitian, lama paparan gelombang elektromagnetik yang berasaldari telepon genggam dalam penelitian ini masih berada dalam tingkat aman untuk mencit bunting.

Analisis Proteomik Cairan Sinovial Sendi Domba: Efektivitas Metode dan Profil Protein Fungsional Kusdiantoro Mohamad; Wiwit Ridhani Rahmaniyah; I Ketut Mudite Adnyane; Mokhamad Fahrudin; Arief Boediono
Acta VETERINARIA Indonesiana Vol. 8 No. 2 (2020): Juli 2020
Publisher : IPB University

Penelitian ini bertujuan untuk mengevaluasi metode deplesi dan digesi protein, serta menganalisis proteom cairan sinovial (SF) sendi pada domba sehat menggunakan kromatografi cair–tandem spektrometri massa. Cairan SF dikoleksi dari enam ekor domba garut betina, umur ±4 tahun, berat 35-40 kg, dan sehat secara klinis. Deplesi protein berkelimpahan tinggi dilakukan dengan metode spin column menggunakan TOP 12 dan metode proteospin, sementara digesi protein dengan tiga metode, yaitu digesi in solution, in gel, dan in-solution + filter-aided sample preparation (FASP). Metode yang terbaik kemudian dilanjutkan dengan fraksinasi peptida menggunakan ultra performance liquid chromatography. Metode deplesi proteospin dengan digesi protein in-solution + FASP merupakan metode terbaik berdasarkan nilai coverage dan sequest HT. Hasil analisis proteomik terkarakterisasi 52 protein pada database spesies domba. Anotasi gen ontologi menggunakan DAVID analysis menunjukkan bahwa protein-protein SF tersebut merupakan komponen sel terutama sebagai eksosom ekstraseluler, fungsi molekuler sebagai aktivitas inhibitor endopeptidase tipe-serin dan pengikat ion kalsium; serta proses biologis sebagai angiogenesis dan koagulasi darah atau pembentukan fibrin. Analisis KEGG pathway menunjukkan protein SF berperan utama pada jalur kaskade koagulasi dan komplemen.

The Influence of Homologous and Heterologous Sera in Culture Medium on in Vitro Maturation and Fertilization of Sheep Oocytes Ita Djuwita; Yohan Rusyiyantono; Kusdiantoro Muhamad; Bambang Purwantara; Yuhara Sukra
Media Veteriner Vol. 5 No. 1 (1998): Media Veteriner
Publisher : Media Veteriner

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (749.792 KB)

The influence of various types of sera i.e. Fetal Calf Serum (FCS), Bovine Post Estrous Serum (SPES) as heterolog sera; and Ewes Fetal Serum (EFS) and Ewes Serum (ES) as homologue sera on in vitro maturation and fertilization of sheep oocytes was investigated using Tissue Culture Medium 199TM (TCM 199. Gibco). Sovine sera were collected from cow at seven days post-estrus, while ewes sera were collected during estrous (ES-HO) and six days post estrous (ES-H6). The result shows that the maturation rate of oocytes cultured in medium supplemented with EFS, ES-HO and ES-H6 at concentrations of 32.9%, 68.7%, and 67.6% respectively is better than those supplemented with FCS and SPES at concentrations of 22.2% and 28.9%, respectively. In vitro fertilization rate were significantly higher in medium supplemented with ES-HO and ES-H6 at concentration of 65.4% and 65.8% (P < 0.05) than that supplemented with SPES at concentration of 31.7%. In conclusion, ewes sera could be used in culture medium for promoting both in vitro maturation and fertilization rate of sheep oocytes.

STUDI PERBANDINGAN EFEK DIURETIK DAN ANTIINFLAMASI GABUNGAN EKSTRAK SIDAGURI, TEMPUYUNG DAN SELEDRI PADA TIKUS Min Rahminiwati; Raudhatul Jannah; Kusdiantoro Mohamad
FITOFARMAKA: Jurnal Ilmiah Farmasi Vol 11, No 2 (2021): FITOFARMAKA | Jurnal Ilmiah Farmasi
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/jf.v11i2.2999

Sidaguri (Sida rhombifiola L.), tempuyung (Sonchus arvensis L.), dan seledri (Apium graveolens L.) dilaporkan mengandung flavonoid yang berperan penting dalam mekanisme terjadinya efek diuretik dan antinflamasi beragam herbal. Namun potensi flavonoid dalam menimbulkan kedua efek tersebut berbeda pada setiap tanaman baik tunggal maupun ramuan. Penelitian ini ditujukan untuk mempelajari kekuatan efek diuretik dan antiinflamasi dari gabungan ketiga tanaman tersebut pada tikus. Lima ekor tikus dari masing masing kelompok uji diuretik dan 4 ekor tikus dari kelompok antiinflamasi diberi aquadest sebagai grup kontrol, ekstrak tempuyung 28 mg/200 g BB, dan kombinasi ketiga tanaman dosis 28 mg/200 g BB, 56 mg/200 g BB, dan 280 mg/200 g BB secara oral selama 8 hari berturut turut. Total volume urin kelompok tikus diuretik diukur pada hari ke 8 sampai dengan hari ke 10 sedangkan volume udem kaki tikus yang diinduksi karagenan kelompok tikus antiinflamasi diukur 30 menit sebelum dan sesudah induksi dan setiap jam selama 6 jam. Data yang diperoleh menunjukkan bahwa sediaan dosis 56 mg/kg BB mempunyai total volume urin dan percent diuretic index lebih besar dari kontrol dan pada dosis 28 mg/kg BB mempunyai nilai AUC terendah serta % DAI tertinggi. Dengan demikian sediaan. mempunyai efek antiinflamasi lebih kuat dibandingkan dengan efek diuretik.

Biological Analysis of Leydig Cells-Conditioned Medium To Support Rat Bone Marrow Mesenchymal Stem Cells Differentiation Ekayanti Mulyawati Kaiin; Wahono Esthi Prasetyaningtyas; Kusdiantoro Mohamad; Ita Djuwita; Tuty Laswardi Yusuf; Mohamad Agus Setiadi
ANNALES BOGORIENSES Vol 22, No 1 (2018): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

The developed Leydig cells-conditioned medium (LCM) contains bioactive materials secreted by Leydig cells in vitro. LCM was used to evaluate the ability of bone marrow mesenchymal stem cells differentiation. Bone marrow mesenchymal stem cells (1x 106 cell/ml) were cultured in : 1) DMEM supplemented with 10% NBCS as a control (M), 2) M supplemented with 10 ng/ml testosterone; 3) M supplemented with 50% LCM ; 4) M supplemented with 50% LCM and 2.5 IU/ml hCG. Bone marrow mesenchymal stem cells that were cultured with LCM has a positive reaction (57.4%) to histochemistry staining 3β-HSD and produced 1.87 ng/ml testosterone. Supplementation of hCG to LCM increased the positive number of Leydig cells and testosterone production by 74.6% and 12.33 ng/ml (P<0.05). It can be concluded that Leydig cells-conditioned medium can support differentiation of bone marrow mesenchymal stem cells into Leydig cells.

Postmortem Examination of Emu Bird (Dromaius novaehollandiae) in Ragunan Zoo Pardede, Friselsa; Rahardi, Nindya Anandaputri; Edwar, Syafri; Mohamad, Kusdiantoro
Jurnal Medik Veteriner Vol. 5 No. 2 (2022): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss2.2022.252-260

Ragunan Zoo is one of the ex-situ conservation sites in DKI Jakarta to protect the wildlife outside their natural habitat. The emu (Dromaius novaehollandiae) is one of the TMR collection animals originating from Australia. An emu bird named Lebo, aged 19 years, and male sex was found sick on January 12, 2022. Based on physical examination, the emu bird showed clinical symptoms of anorexia, unwilling to eat, lethargic, refusing or difficult to move, unable to lift his head, and inflammation of the right eye. No further examination was performed to determine the diagnosis. Treatments were taken symptomatically, namely the fluid therapy, injection of vitamins and energy sources, antibiotics, and anti-inflammatory. The emu was found dead on February 6, 2022 and a necropsy was performed. Postmortem findings obtained were glossitis, ventriculitis, gizzard erosion, hemorrhagic enteritis, hepatic lipidosis, epicarditis, testicular atrophy, and orchitis. Diagnosis of suspected cause of death is damage and decreased function of various organs of the body.

Co-Authors . NURBARIAH A.S. Satyaningtijas Aditianingrum, Kurnia Alysia Ali, Ihfan Khaidir Andriyanto Andriyanto Arief Boediono Aryani, Nursela Sofyanti Mirza Bambang Purwantara Damiana Rita Ekastuti Deny Putra Romadhon Edwar, Syafri Ekayanti Mulyawati Kaiin Erdiansyah Rahmi Faisal Mustafa Faralinda Sari Fitri Susana Gatot Prasojo Gustini Syahbirin Hamny Sofyan Hardiansyah, Muhamad Evans Harry Murti HERA MAHESHWARI Husna, Hanifati Iman Supriatna Ita Djuwita ITA DJUWITA ITA DJUWITA Ita Djuwita Ita Djuwita Kaiin, Ekayanti Mulyawati Kaiin, Ekayanti Mulyawati Ketut Adnyane Mudite Lubis, Andri Maruli Tua M Agus Setiadi Mahendra, Arindina Mokhamad Fahrudin Muhammad Jalaluddin, Muhammad Ni Wayan Kurniani Karja Nurhidayat . Nurhidayat Nurhidayat Pardede, Friselsa Perdhana, Ika Satya R. Iis Arifiantini Rahardi, Nindya Anandaputri Rahmadhani, Elsi Rahmaniyah, Wiwit Ridhani Rahminiwati, Min Rakhmawati, Handina Raudhatul Jannah Ritonga, Mudhita Zikrullah Situmeang, Adrian Sri Wahyuni Srihadi Agungpriyono Sulistyana, Yanti Tanjung, Fitri Aisah Tarigan, Ronald Taufan Hidayat TUTY LASWARDI YUSUF Tuty Laswardi Yusuf Vincentia Maria Vivit Riyacumala Wahono Esthi Prasetyaningtijas Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wardhani, Lailia Dwi Kusuma Wasmen Manalu Wiwit Ridhani Rahmaniyah Yohan Rusiyanto Yuhara Sukra

Title

Found 18 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 18 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 18 Documents
Search