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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Media Veteriner Jurnal Sain Veteriner Jurnal Veteriner ANNALES BOGORIENSES Jurnal Medik Veteriner ARSHI Veterinary Letters FITOFARMAKA: Jurnal Ilmiah Farmasi Current Biomedicine Annales Bogorienses
Kusdiantoro Mohamad
Fakultas Kedokteran Hewan Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Pertumbuhan dan Sekresi Protein Hasil Kultur Primer Sel-Sel Serebrum Anak Tikus (IN VITRO GROWTH AND PROTEIN SECRETION OF NEWBORN RAT CEREBRAL PRIMARY CELLS CULTURE) Ita Djuwita; Vivit Riyacumala; Kusdiantoro Mohamad; Wahono Esthi Prasetyaningtijas; Nurhidayat .
Jurnal Veteriner Vol 13 No 2 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (206.246 KB)

Abstract

The objective of this study was to identify the proliferation and differentiation of mice cerebrum cellsand its protein product. Research has been conducted on in vitro growth of three days old rat cerebrum cellsin Dulbecco’s Modified Eagle Medium (DMEM) containing 10% Amino Acid Non Essential (AANE), 10%Fetal Bovine Serum (FBS), 3mM NaHCO3, 50 mg/ml gentamycin, and supplemented with and without 5mg/ml insulin, 10 mg/ml transferin, 5 mg/ml selenium (ITS). Culture was done in 5% CO2, then incubatedat 37oC until 90% confluent. Identification were done on cell proliferation and population doubling time(PDT), number of neuron and glial cells, length of axon and dendrit, and protein secretion. The number andlength of neuronal cells were calculated by using hemocytometer and eyepiece micrometer, respectivelly.Protein secreted into culture medium, designed as conditioned medium (CM) was analysized using sodiumdodecyl sulfate–polyacrilamide gel electrophoresis (SDS-PAGE) method. Quantitative data were analyzedusing statistical T-test on Minitab program. In vitro culture of rat cerebrum cells showed two types of cellsincluding nerve cells of bipolar and multipolar neurons and glial cells including astrocyte (the fibrous andprotoplasmic), oligodendrocyte, and microglia. Supplementation of ITS into the culture medium increasedthe cell proliferation rate (P<0.05) with lower PDT, and quantitatively, the 30 kDa secreted protein asindicated by the higher intensity of the protein band.
STUDI PERBANDINGAN EFEK DIURETIK DAN ANTIINFLAMASI GABUNGAN EKSTRAK SIDAGURI, TEMPUYUNG DAN SELEDRI PADA TIKUS Min Rahminiwati; Raudhatul Jannah; Kusdiantoro Mohamad
FITOFARMAKA: Jurnal Ilmiah Farmasi Vol 11, No 2 (2021): FITOFARMAKA | Jurnal Ilmiah Farmasi
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/jf.v11i2.2999

Abstract

Sidaguri (Sida rhombifiola L.), tempuyung (Sonchus arvensis L.), dan seledri (Apium graveolens L.) dilaporkan mengandung flavonoid yang berperan penting dalam mekanisme terjadinya efek diuretik dan antinflamasi beragam herbal. Namun potensi flavonoid dalam menimbulkan kedua efek tersebut berbeda pada setiap tanaman baik tunggal maupun ramuan. Penelitian ini ditujukan untuk mempelajari kekuatan efek diuretik dan antiinflamasi dari gabungan ketiga tanaman tersebut pada tikus. Lima ekor tikus dari masing masing kelompok uji diuretik dan 4 ekor tikus dari kelompok antiinflamasi diberi aquadest sebagai grup kontrol, ekstrak tempuyung 28 mg/200 g BB, dan kombinasi ketiga tanaman dosis 28 mg/200 g BB, 56 mg/200 g BB, dan 280 mg/200 g BB secara oral selama 8 hari berturut turut. Total volume urin kelompok tikus diuretik diukur pada hari ke 8 sampai dengan hari ke 10 sedangkan volume udem kaki tikus yang diinduksi karagenan kelompok tikus antiinflamasi diukur 30 menit sebelum dan sesudah induksi dan setiap jam selama 6 jam. Data yang diperoleh menunjukkan bahwa sediaan dosis 56 mg/kg BB mempunyai total volume urin dan percent diuretic index lebih besar dari kontrol dan pada dosis 28 mg/kg BB mempunyai nilai AUC terendah serta % DAI tertinggi. Dengan demikian sediaan. mempunyai efek antiinflamasi lebih kuat dibandingkan dengan efek diuretik.
Biological Analysis of Leydig Cells-Conditioned Medium To Support Rat Bone Marrow Mesenchymal Stem Cells Differentiation Ekayanti Mulyawati Kaiin; Wahono Esthi Prasetyaningtyas; Kusdiantoro Mohamad; Ita Djuwita; Tuty Laswardi Yusuf; Mohamad Agus Setiadi
ANNALES BOGORIENSES Vol 22, No 1 (2018): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (457.602 KB) | DOI: 10.14203/ann.bogor.2018.v22.n1.20-26

Abstract

The developed Leydig cells-conditioned medium (LCM) contains bioactive materials secreted by Leydig cells in vitro.  LCM was used to evaluate the ability of bone marrow mesenchymal stem cells differentiation. Bone marrow mesenchymal stem cells (1x 106 cell/ml) were cultured in : 1) DMEM supplemented with 10% NBCS as a control (M), 2) M supplemented with 10 ng/ml testosterone; 3) M supplemented with 50%  LCM ; 4) M supplemented with 50% LCM and 2.5 IU/ml hCG. Bone marrow mesenchymal stem cells that were cultured with  LCM has a positive reaction (57.4%) to histochemistry staining 3β-HSD and produced 1.87 ng/ml testosterone. Supplementation of hCG to LCM  increased the positive number of Leydig cells and testosterone production by 74.6% and 12.33 ng/ml (P<0.05). It can be concluded that Leydig cells-conditioned medium can support differentiation of bone marrow mesenchymal stem cells into Leydig cells.
Perbedaan Morfologi dan Ekspresi Dazl dan Vasa pada Sel Germinal Fetus dan Anak Mencit Jantan Wahono Esthi Prasetyaningtyas; Ni Wayan Kurniani Karja; Mokhamad Fahrudin; Kusdiantoro Mohamad; Srihadi Agungpriyono
Jurnal Veteriner Vol 24 No 1 (2023)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2023.24.1.49

Abstract

Sel germinal merupakan salah satu sumber sel yang masih bersifat totipotensi dan berperan dalam pembentukan organisme baru. Morfologi dan ekspresi protein pada sel germinal bersifat dinamis bergantung pada umur dan tahap perkembangan. Penelitian ini bertujuan untuk mengamati perubahan morfologi dan ekspresi protein sebagai marka sel germinal jantan pada fetus umur 13,5 hari pascakawin (days post coital/ dpc) dan anak mencit umur lima hari pascalahir. Hasil Rigi kelamin dan testis diisolasi dari mencit umur 13,5 dpc dan 5 hari. Jaringan kemudian dipreparasi histologi rutin, dan diwarnai dengan pewarnaan hematoksilineosin (HE), sedangkan untuk mengidentifikasi keberadaan protein Dazl, Vasa dan Oct4, jaringan diwarnai dengan pewarnaan imunohistokimia menunjukkan morfologi sel germinal jantan pada fetus mencit umur 13,5 dpc dan anak mencit umur lima hari pascalahir sama-sama berbentuk bulat oval. Namun, sel germinal jantan pada mencit umur lima hari pascalahir berukuran lebih besar, jumlah yang lebih sedikit dan terletak jauh dari membran basal. Pada sel germinal jantan umur 13,5 dpc menunjukkan positif lemah terhadap antibodi Oct 4 dan DAZL serta positif kuat terhadap antibodi Vasa. Pada umur lima hari, sel germinal jantan menunjukkan positif kuat terhadap antibodi Oct-4 dan DAZL, serta positif lemah terhadap antibodi Vasa. Simpulan dari penelitian ini adalah morfologi dan ekspresi marka sel germinal dipengaruhi oleh tahapan pertumbuhan dan perkembangan sel-sel germinal. Vasa dapat digunakan sebagai marka untuk sel germinal umur 13,5 dpc dan DAZL sebagai marka untuk sel germinal umur lima hari pascalahir.
Postmortem Examination of Emu Bird (Dromaius novaehollandiae) in Ragunan Zoo Pardede, Friselsa; Rahardi, Nindya Anandaputri; Edwar, Syafri; Mohamad, Kusdiantoro
Jurnal Medik Veteriner Vol. 5 No. 2 (2022): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss2.2022.252-260

Abstract

Ragunan Zoo is one of the ex-situ conservation sites in DKI Jakarta to protect the wildlife outside their natural habitat. The emu (Dromaius novaehollandiae) is one of the TMR collection animals originating from Australia. An emu bird named Lebo, aged 19 years, and male sex was found sick on January 12, 2022. Based on physical examination, the emu bird showed clinical symptoms of anorexia, unwilling to eat, lethargic, refusing or difficult to move, unable to lift his head, and inflammation of the right eye. No further examination was performed to determine the diagnosis. Treatments were taken symptomatically, namely the fluid therapy, injection of vitamins and energy sources, antibiotics, and anti-inflammatory. The emu was found dead on February 6, 2022 and a necropsy was performed. Postmortem findings obtained were glossitis, ventriculitis, gizzard erosion, hemorrhagic enteritis, hepatic lipidosis, epicarditis, testicular atrophy, and orchitis. Diagnosis of suspected cause of death is damage and decreased function of various organs of the body.
Acute Toxicity of Ethanol Extract of Curcuma zedoaria Rosc (Zingiberaceae) Rhizomes on Brine Shrimp Larvae and Zebrafish Embryos Syahbirin, Gustini; Aditianingrum, Kurnia Alysia; Mohamad, Kusdiantoro
Jurnal Medik Veteriner Vol. 7 No. 1 (2024): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss1.2024.7-18

Abstract

Curcuma zedoaria Rosc (Zingiberaceae), known as white turmeric or temu putih, is commonly used as traditional medicine in Indonesia. The toxicity of this herb needs to be studied to improve its application. This study aimed to evaluate the toxicity of the crude ethanol extract of temu putih rhizome against brine shrimp (Artemia salina) larvae and zebrafish (Danio rerio) embryos and to analyze the potential active compounds contained in the extract. The crude ethanol extract showed a 50% lethal concentration value (LC50) at 588 ppm against brine shrimp larvae and 224 ppm against zebrafish embryos. Based on the gas chromatography-mass spectrometry analysis, the suspected active compounds that play a role in the toxicity were epicurzerenone, curzerene, and curzerenone, while 2,4,6-trimethylacetophenone was the predominant compound.
Effect of moringa seed (Moringa oleifera Lam.) infusion on stamina performance in male mice Husna, Hanifati; Andriyanto, Andriyanto; Manalu, Wasmen; Mohamad, Kusdiantoro
Current Biomedicine Vol. 3 No. 2 (2025): July
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.3.2.67

Abstract

Background Individuals experience varying levels of fatigue and stamina. Moringa oleifera Lam. Seed extract relieves fatigue due to its high levels of antioxidants and glucosinolate glucomoringin, which reduces intracellular oxidative stress. Objectives This study aimed to determine the effectiveness of infusion of M. oleifera seed as a stamina stimulator in mice. Methods This experiment used 25 male mice divided into five groups (five mice in each group), consisting of M. oleifera seed infusion at doses of 0, 1, 3, and 5 g/kg BW as the negative control and treatment groups, and caffeine at a dose of 6.5 mg/kg BW as the positive control group. Stamina effectiveness was evaluated using a swimming endurance test that measured swimming duration and physiological parameters every 15 min for 2 h after administration. Data were analyzed using analysis of variance (ANOVA) followed by Tukey’s test. Results M. oleifera seed infusion at a dose of 3 g/kg BW had the shortest swimming duration difference (Δ) value, with no difference compared to the 5 g/kg BW and caffeine groups, but was significantly different (P<0.05) compared to the 0 and 1 g/kg BW groups. M. oleifera seed infusion at a dose of 3 g/kg BW also showed a lower delta value for body surface temperature and heartbeat rate compared to the negative control, and a lower delta value for the respiratory rate compared to the 5 g/kg BW group. Conclusion M. oleifera seed infusion potentially maintains stamina performance in mice at a dose of 3 g/kg BW.
Neurogical deficit score dan gambaran hematologi tikus model strok iskemik dengan metode middle cerebral artery occlusion Perdhana, Ika Satya; Hardiansyah, Muhamad Evans; Maheshwari, Hera; Mohamad, Kusdiantoro
ARSHI Veterinary Letters Vol. 9 No. 3 (2025): ARSHI Veterinary Letters - August 2025
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.9.3.83-84

Abstract

Stroke is a neurological disorder caused by impaired blood flow to specific regions of the brain, often resulting in paralysis and functional deficits in the affected area. Among its subtypes, ischemic stroke is the most prevalent in the general population. This study aimed to establish a rat model of ischemic stroke induced by middle cerebral artery occlusion (MCAO). Eight male Sprague-Dawley rats aged 8–10 weeks were randomly assigned to two groups: control and treatment group. In the treatment group, the middle cerebral artery was occluded for 45 min. Neurological assessment using the Bederson scale revealed a neurological deficit score (NDS) of 1, characterized by contralateral flexion after left-brain injury. Hematological analysis demonstrated significantly higher white blood cell (WBC) counts (p < 0.05), whereas red blood cell (RBC) counts, hemoglobin concentration, and hematocrit values were significantly lower (p < 0.05) than those in the control group. These findings indicate that the MCAO method with a 45-minute occlusion successfully induced a mild neurological deficit accompanied by distinct hematological alterations, thereby providing a reproducible animal model for ischemic stroke research.
The Expression of Type III Collagen Connective Tissue inGluteobiceps and Psoas Mayor Muscles of Aceh Cattle Tanjung, Fitri Aisah; Ali, Ihfan Khaidir; Jalaluddin, Muhammad; Wahyuni, Sri; Sabri, Mustafa; Hidayat, Taufan; Wardhani, Lailia Dwi Kusuma; Ritonga, Mudhita Zikrullah; Rahmi, Erdiansyah; Prasetyaningtyas, Wahono Esthi; Mohamad, Kusdiantoro; Sofyan, Hamny
Acta VETERINARIA Indonesiana Vol. 13 No. 2 (2025): Juli 2025
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.13.2.%p

Abstract

Collagen is one of the determinant factors of beef tenderness. This study analyzed the expression and content of type III collagen connective tissue in two muscle types of Aceh cattle through immunohistochemical staining. Meat samples were obtained from the gluteobiceps and psoas major muscles of three Aceh cattle bulls weighing 250-300 kg, with a body condition score of three. Histology preparations were created from samples and subjected to immunohistochemical staining utilizing specific antibodies targeting type III collagen. Qualitative data were analyzed descriptively, while quantitative data were analyzed using IMAGE J software. The results showed that the expression of type III collagen connective tissue was found in the endomysium and perimysium layers of both muscles. The expression of type III collagen connective tissue varied significantly among the muscles (P<0.05). The gluteobiceps muscle had an average percentage of the area containing type III collagen connective tissue of 4.47±2.41%, while the psoas major muscle was lower at 2.51±1.69%. This study concludes that the gluteobiceps muscle of Aceh cattle has a higher amount of type III collagen connective tissue than the psoas major muscle.
Efek Campuran Ekstrak Temulawak (Curcuma xanthorrhiza) dan Temuputih (Curcuma zedoaria) terhadap Toksisitas Akut pada Embrio Ikan Zebra Mohamad, Kusdiantoro; Aryani, Nursela Sofyanti Mirza; Prasetyaningtyas, Wahono Esthi; Syahbirin, Gustini
Jurnal Sain Veteriner Vol 42, No 2 (2024): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.94142

Abstract

Temulawak (Curcuma xanthorrhiza) dan temuputih (Curcuma zedoaria) termasuk tanaman herbal yang mempunyai potensi sebagai antitumor dan antikanker. Beberapa obat antikanker dan ekstrak herbal telah dilaporkan memiliki toksisitas yang tinggi dan menyebabkan malformasi pada embrio ikan zebra (Danio rerio). Penelitian ini bertujuan membandingkan toksisitas ekstrak etanol C. xanthorrhiza, C. zedoaria, dan campurannya (rasio 1:6) terhadap embrio ikan zebra. Rimpang C. xanthorrhiza dan C. zedoaria diekstrak dengan cara maserasi dalam etanol 80%. Ekstrak diuji toksisitas akut dengan embrio ikan zebra menggunakan metode OECD Guidelines for the Testing of Chemicals No. 236 (2013). Nilai lethal concentration 50 (LC50) campuran ekstrak adalah 82.9 ppm, berada di antara nilai LC50 masing-masing ekstrak (16.9 untuk C. xanthorrhiza dan 112.1 untuk C. zedoaria). Tingkat menetas embrio rendah pada ekstrak C. xanthorrhiza tetapi tinggi pada ekstrak C. zedoaria dan ekstrak campuran. Kelainan notokorda dan edema kantong kuning telur tinggi pada ekstrak C. xanthorrhiza tetapi rendah pada ekstrak C. zedoaria dan ekstrak campuran. Kelainan sirkulasi darah rendah pada ekstrak C. zedoaria tetapi tinggi pada ekstrak campuran. Edema perikardium dan koagulasi darah merupakan malformasi mayor pada semua ekstrak. Dapat disimpulkan bahwa campuran ekstrak etanol temulawak dan temuputih menyebabkan malformasi edema perikardium, koagulasi darah, dan secara sinergis menghambat sirkulasi darah, yang berkaitan dengan potensi antikanker. Selain itu, ekstrak campuran dapat menurunkan hambatan menetas, malformasi notokorda, dan edema kantong kuning telur.
Co-Authors . NURBARIAH A.S. Satyaningtijas Aditianingrum, Kurnia Alysia Ali, Ihfan Khaidir Andriyanto Andriyanto Arief Boediono Aryani, Nursela Sofyanti Mirza Bambang Purwantara Damiana Rita Ekastuti Deny Putra Romadhon Edwar, Syafri Ekayanti Mulyawati Kaiin Erdiansyah Rahmi Faisal Mustafa Faralinda Sari Fitri Susana Gatot Prasojo Gustini Syahbirin Hamny Sofyan Hardiansyah, Muhamad Evans HERA MAHESHWARI Husna, Hanifati Iman Supriatna Ita Djuwita ITA DJUWITA ITA DJUWITA Ita Djuwita Ita Djuwita Kaiin, Ekayanti Mulyawati Kaiin, Ekayanti Mulyawati Ketut Adnyane Mudite M Agus Setiadi Mahendra, Arindina Mokhamad Fahrudin Muhammad Jalaluddin, Muhammad Ni Wayan Kurniani Karja Nurhidayat . Pardede, Friselsa Perdhana, Ika Satya R. Iis Arifiantini Rahardi, Nindya Anandaputri Rahmadhani, Elsi Rahmaniyah, Wiwit Ridhani Rahminiwati, Min Raudhatul Jannah Ritonga, Mudhita Zikrullah Sri Wahyuni Srihadi Agungpriyono Sulistyana, Yanti Tanjung, Fitri Aisah Tarigan, Ronald Taufan Hidayat TUTY LASWARDI YUSUF Tuty Laswardi Yusuf Vincentia Maria Vivit Riyacumala Wahono Esthi Prasetyaningtijas Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wardhani, Lailia Dwi Kusuma Wasmen Manalu Wiwit Ridhani Rahmaniyah Yohan Rusiyanto Yuhara Sukra