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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Media Veteriner Jurnal Sain Veteriner ANNALES BOGORIENSES Jurnal Medik Veteriner ARSHI Veterinary Letters FITOFARMAKA: Jurnal Ilmiah Farmasi Current Biomedicine Annales Bogorienses
Kusdiantoro Mohamad
Fakultas Kedokteran Hewan Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Acute Toxicity of Ethanol Extract of Curcuma zedoaria Rosc (Zingiberaceae) Rhizomes on Brine Shrimp Larvae and Zebrafish Embryos Syahbirin, Gustini; Aditianingrum, Kurnia Alysia; Mohamad, Kusdiantoro
Jurnal Medik Veteriner Vol. 7 No. 1 (2024): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss1.2024.7-18

Abstract

Curcuma zedoaria Rosc (Zingiberaceae), known as white turmeric or temu putih, is commonly used as traditional medicine in Indonesia. The toxicity of this herb needs to be studied to improve its application. This study aimed to evaluate the toxicity of the crude ethanol extract of temu putih rhizome against brine shrimp (Artemia salina) larvae and zebrafish (Danio rerio) embryos and to analyze the potential active compounds contained in the extract. The crude ethanol extract showed a 50% lethal concentration value (LC50) at 588 ppm against brine shrimp larvae and 224 ppm against zebrafish embryos. Based on the gas chromatography-mass spectrometry analysis, the suspected active compounds that play a role in the toxicity were epicurzerenone, curzerene, and curzerenone, while 2,4,6-trimethylacetophenone was the predominant compound.
Effect of moringa seed (Moringa oleifera Lam.) infusion on stamina performance in male mice Husna, Hanifati; Andriyanto, Andriyanto; Manalu, Wasmen; Mohamad, Kusdiantoro
Current Biomedicine Vol. 3 No. 2 (2025): July
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.3.2.67

Abstract

Background Individuals experience varying levels of fatigue and stamina. Moringa oleifera Lam. Seed extract relieves fatigue due to its high levels of antioxidants and glucosinolate glucomoringin, which reduces intracellular oxidative stress. Objectives This study aimed to determine the effectiveness of infusion of M. oleifera seed as a stamina stimulator in mice. Methods This experiment used 25 male mice divided into five groups (five mice in each group), consisting of M. oleifera seed infusion at doses of 0, 1, 3, and 5 g/kg BW as the negative control and treatment groups, and caffeine at a dose of 6.5 mg/kg BW as the positive control group. Stamina effectiveness was evaluated using a swimming endurance test that measured swimming duration and physiological parameters every 15 min for 2 h after administration. Data were analyzed using analysis of variance (ANOVA) followed by Tukey’s test. Results M. oleifera seed infusion at a dose of 3 g/kg BW had the shortest swimming duration difference (Δ) value, with no difference compared to the 5 g/kg BW and caffeine groups, but was significantly different (P<0.05) compared to the 0 and 1 g/kg BW groups. M. oleifera seed infusion at a dose of 3 g/kg BW also showed a lower delta value for body surface temperature and heartbeat rate compared to the negative control, and a lower delta value for the respiratory rate compared to the 5 g/kg BW group. Conclusion M. oleifera seed infusion potentially maintains stamina performance in mice at a dose of 3 g/kg BW.
Neurogical deficit score dan gambaran hematologi tikus model strok iskemik dengan metode middle cerebral artery occlusion Perdhana, Ika Satya; Hardiansyah, Muhamad Evans; Maheshwari, Hera; Mohamad, Kusdiantoro
ARSHI Veterinary Letters Vol. 9 No. 3 (2025): ARSHI Veterinary Letters - August 2025
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.9.3.83-84

Abstract

Stroke is a neurological disorder caused by impaired blood flow to specific regions of the brain, often resulting in paralysis and functional deficits in the affected area. Among its subtypes, ischemic stroke is the most prevalent in the general population. This study aimed to establish a rat model of ischemic stroke induced by middle cerebral artery occlusion (MCAO). Eight male Sprague-Dawley rats aged 8–10 weeks were randomly assigned to two groups: control and treatment group. In the treatment group, the middle cerebral artery was occluded for 45 min. Neurological assessment using the Bederson scale revealed a neurological deficit score (NDS) of 1, characterized by contralateral flexion after left-brain injury. Hematological analysis demonstrated significantly higher white blood cell (WBC) counts (p < 0.05), whereas red blood cell (RBC) counts, hemoglobin concentration, and hematocrit values were significantly lower (p < 0.05) than those in the control group. These findings indicate that the MCAO method with a 45-minute occlusion successfully induced a mild neurological deficit accompanied by distinct hematological alterations, thereby providing a reproducible animal model for ischemic stroke research.
The Expression of Type III Collagen Connective Tissue inGluteobiceps and Psoas Mayor Muscles of Aceh Cattle Tanjung, Fitri Aisah; Ali, Ihfan Khaidir; Jalaluddin, Muhammad; Wahyuni, Sri; Sabri, Mustafa; Hidayat, Taufan; Wardhani, Lailia Dwi Kusuma; Ritonga, Mudhita Zikrullah; Rahmi, Erdiansyah; Prasetyaningtyas, Wahono Esthi; Mohamad, Kusdiantoro; Sofyan, Hamny
Acta VETERINARIA Indonesiana Vol. 13 No. 2 (2025): Juli 2025
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.13.2.112-118

Abstract

Collagen is one of the determinant factors of beef tenderness. This study analyzed the expression and content of type III collagen connective tissue in two muscle types of Aceh cattle through immunohistochemical staining. Meat samples were obtained from the gluteobiceps and psoas major muscles of three Aceh cattle bulls weighing 250-300 kg, with a body condition score of three. Histology preparations were created from samples and subjected to immunohistochemical staining utilizing specific antibodies targeting type III collagen. Qualitative data were analyzed descriptively, while quantitative data were analyzed using IMAGE J software. The results showed that the expression of type III collagen connective tissue was found in the endomysium and perimysium layers of both muscles. The expression of type III collagen connective tissue varied significantly among the muscles (P<0.05). The gluteobiceps muscle had an average percentage of the area containing type III collagen connective tissue of 4.47±2.41%, while the psoas major muscle was lower at 2.51±1.69%. This study concludes that the gluteobiceps muscle of Aceh cattle has a higher amount of type III collagen connective tissue than the psoas major muscle.
Efek Campuran Ekstrak Temulawak (Curcuma xanthorrhiza) dan Temuputih (Curcuma zedoaria) terhadap Toksisitas Akut pada Embrio Ikan Zebra Mohamad, Kusdiantoro; Aryani, Nursela Sofyanti Mirza; Prasetyaningtyas, Wahono Esthi; Syahbirin, Gustini
Jurnal Sain Veteriner Vol 42, No 2 (2024): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.94142

Abstract

Temulawak (Curcuma xanthorrhiza) dan temuputih (Curcuma zedoaria) termasuk tanaman herbal yang mempunyai potensi sebagai antitumor dan antikanker. Beberapa obat antikanker dan ekstrak herbal telah dilaporkan memiliki toksisitas yang tinggi dan menyebabkan malformasi pada embrio ikan zebra (Danio rerio). Penelitian ini bertujuan membandingkan toksisitas ekstrak etanol C. xanthorrhiza, C. zedoaria, dan campurannya (rasio 1:6) terhadap embrio ikan zebra. Rimpang C. xanthorrhiza dan C. zedoaria diekstrak dengan cara maserasi dalam etanol 80%. Ekstrak diuji toksisitas akut dengan embrio ikan zebra menggunakan metode OECD Guidelines for the Testing of Chemicals No. 236 (2013). Nilai lethal concentration 50 (LC50) campuran ekstrak adalah 82.9 ppm, berada di antara nilai LC50 masing-masing ekstrak (16.9 untuk C. xanthorrhiza dan 112.1 untuk C. zedoaria). Tingkat menetas embrio rendah pada ekstrak C. xanthorrhiza tetapi tinggi pada ekstrak C. zedoaria dan ekstrak campuran. Kelainan notokorda dan edema kantong kuning telur tinggi pada ekstrak C. xanthorrhiza tetapi rendah pada ekstrak C. zedoaria dan ekstrak campuran. Kelainan sirkulasi darah rendah pada ekstrak C. zedoaria tetapi tinggi pada ekstrak campuran. Edema perikardium dan koagulasi darah merupakan malformasi mayor pada semua ekstrak. Dapat disimpulkan bahwa campuran ekstrak etanol temulawak dan temuputih menyebabkan malformasi edema perikardium, koagulasi darah, dan secara sinergis menghambat sirkulasi darah, yang berkaitan dengan potensi antikanker. Selain itu, ekstrak campuran dapat menurunkan hambatan menetas, malformasi notokorda, dan edema kantong kuning telur.
Biological Analysis of Leydig Cells-Conditioned Medium to Support Rat Bone Marrow Mesenchymal Stem Cells Differentiation Kaiin, Ekayanti Mulyawati; Prasetyaningtyas, Wahono Esthi; Mohamad, Kusdiantoro; Djuwita, Ita; Yusuf, Tuty Laswardi; Setiadi, Mohamad Agus
Annales Bogorienses Vol. 22 No. 1 (2018): Annales Bogorienses
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The developed Leydig cells-conditioned medium (LCM) contains bioactive materials secreted by Leydig cells in vitro. LCM was used to evaluate the ability of bone marrow mesenchymal stem cells differentiation. Bone marrow mesenchymal stem cells (1x 106 cell/ml) were cultured in : 1) DMEM supplemented with 10% NBCS as a control (M), 2) M supplemented with 10 ng/ml testosterone; 3) M supplemented with 50% LCM ; 4) M supplemented with 50% LCM and 2.5 IU/ml hCG. Bone marrow mesenchymal stem cells that were cultured with LCM has a positive reaction (57.4%) to histochemistry staining 3β-HSD and produced 1.87 ng/ml testosterone. Supplementation of hCG to LCM increased the positive number of Leydig cells and testosterone production by 74.6% and 12.33 ng/ml (P<0.05). It can be concluded that Leydig cells-conditioned medium can support differentiation of bone marrow mesenchymal stem cells into Leydig cells.
Effects of Physalis angulata Leaf Extract on Female Reproductive Organs Following Busulfan Injection in Rats Satyaningtijas, Aryani; Ekastuti, Damiana Rita; Tarigan, Ronald; Prasetyaningtyas, Wahono Esthi; Adnyane, I Ketut Mudite; Mohamad, Kusdiantoro; Rahmadhani, Elsi; Sulistyana, Yanti; Mahendra, Arindina
HAYATI Journal of Biosciences Vol. 33 No. 2 (2026): March 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.2.490-498

Abstract

Physalis angulata L. (ciplukan) is traditionally used as an herbal remedy with anticancer and antioxidant properties. At the same time, busulfan, a chemotherapy alkylating agent, causes gonadotoxicity and oxidative stress that impair female reproduction. This study aimed to evaluate the effect of P. angulata on reproductive function after busulfan administration in female rats. Twenty-five 6-week-old female rats were randomly divided into: Group I (control), Group II (busulfan only), Group III (P. angulata only), Group IV (busulfan followed by P. angulata after 14 days), and Group V (busulfan and P. angulata administered for 28 days). FSHR and LHR expression in the ovaries and MDA levels in the ovaries and uterus were measured to assess reproductive changes. Busulfan showed no significant effect on FSHR and LHR, whereas P. angulata induced downregulation, suggesting a potential negative feedback mechanism on ovarian receptors. Ovarian MDA showed a decreasing trend with combined treatments, while uterine MDA peaked after busulfan but declined markedly with P. angulata, indicating its role in alleviating oxidative stress. In conclusion, P. angulata may modulate hormonal balance in rat ovaries and decrease oxidative stress in the uterus after busulfan treatment.
Short-term treatment of osteoarthritis-induced sheep with human umbilical cord–derived mesenchymal stem cells: A morphological and proteomic analysis Mohamad, Kusdiantoro; Situmeang, Adrian; Rakhmawati, Handina; Adnyane, I Ketut Mudite; Fahrudin, Mokhamad; Nurhidayat, Nurhidayat; Murti, Harry; Lubis, Andri Maruli Tua; Boediono, Arief
Jurnal Medik Veteriner Vol. 9 No. 1 (2026): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol9.iss1.2026.106-123

Abstract

Knee joints are the primary sites affected by osteoarthritis (OA) in humans and animals. Although intra-articular human umbilical cord–derived mesenchymal stem cells (hUC-MSCs) show therapeutic promise, their combined use with hyaluronic acid (HA) remains underexplored in large animals. This study evaluated morphological and proteomic changes in a sheep osteoarthritis model treated with both agents. A total of twelve sheep were divided into four groups (n = 3 per group): control (normal and OA) and three treatment groups receiving hUC-MSCs, HA, or a combination of HA and hUC-MSCs (HA + hUC-MSCs). OA was induced by meniscectomy, and intra-articular injections were administered four weeks post-surgery. Macroscopic scoring was conducted according to the Osteoarthritis Research Society International system, while histological evaluation was performed using hematoxylin–eosin, Safranin O, and Masson’s trichrome staining. Proteomic analysis of synovial fluid was carried out using liquid chromatography–tandem mass spectrometry. The macroscopic OA score in the HA + hUC-MSC group was lower than in the other treatment groups and was not significantly different from the normal group (p > 0.05). Microscopic observations indicated that hUC-MSCs supported joint cartilage regeneration by preserving proteoglycan and collagen content. Proteomic profiling revealed that treatment with HA + hUC-MSCs modulated proteins associated with complement activation and coagulation pathways. The combination of HA and hUC-MSCs provided synergistic benefits for cartilage regeneration in a sheep OA model during a short treatment period. These findings suggest that this approach represents a promising regenerative therapy for OA in large animals within veterinary medicine and has translational relevance for human regenerative medicine.
Co-Authors . NURBARIAH A.S. Satyaningtijas Aditianingrum, Kurnia Alysia Ali, Ihfan Khaidir Andriyanto Andriyanto Arief Boediono Aryani, Nursela Sofyanti Mirza Bambang Purwantara Damiana Rita Ekastuti Deny Putra Romadhon Edwar, Syafri Ekayanti Mulyawati Kaiin Erdiansyah Rahmi Faisal Mustafa Faralinda Sari Fitri Susana Gatot Prasojo Gustini Syahbirin Hamny Sofyan Hardiansyah, Muhamad Evans Harry Murti HERA MAHESHWARI Husna, Hanifati Iman Supriatna Ita Djuwita Ita Djuwita ITA DJUWITA ITA DJUWITA Ita Djuwita Kaiin, Ekayanti Mulyawati Kaiin, Ekayanti Mulyawati Ketut Adnyane Mudite Lubis, Andri Maruli Tua M Agus Setiadi Mahendra, Arindina Mokhamad Fahrudin Muhammad Jalaluddin, Muhammad Ni Wayan Kurniani Karja Nurhidayat . Nurhidayat Nurhidayat Pardede, Friselsa Perdhana, Ika Satya R. Iis Arifiantini Rahardi, Nindya Anandaputri Rahmadhani, Elsi Rahmaniyah, Wiwit Ridhani Rahminiwati, Min Rakhmawati, Handina Raudhatul Jannah Ritonga, Mudhita Zikrullah Situmeang, Adrian Sri Wahyuni Srihadi Agungpriyono Sulistyana, Yanti Tanjung, Fitri Aisah Tarigan, Ronald Taufan Hidayat TUTY LASWARDI YUSUF Tuty Laswardi Yusuf Vincentia Maria Vivit Riyacumala Wahono Esthi Prasetyaningtijas Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wardhani, Lailia Dwi Kusuma Wasmen Manalu Wiwit Ridhani Rahmaniyah Yohan Rusiyanto Yuhara Sukra