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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Media Veteriner Jurnal Sain Veteriner BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Medik Veteriner ARSHI Veterinary Letters FITOFARMAKA: Jurnal Ilmiah Farmasi Current Biomedicine Jurnal Veteriner
Kusdiantoro Mohamad
Division Of Anatomy, Histology, And Embryology, School Of Veterinary Medicine And Biomedical Sciences, IPB University, Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Acute Toxicity of Ethanol Extract of Curcuma zedoaria Rosc (Zingiberaceae) Rhizomes on Brine Shrimp Larvae and Zebrafish Embryos Syahbirin, Gustini; Aditianingrum, Kurnia Alysia; Mohamad, Kusdiantoro
Jurnal Medik Veteriner Vol. 7 No. 1 (2024): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss1.2024.7-18

Abstract

Curcuma zedoaria Rosc (Zingiberaceae), known as white turmeric or temu putih, is commonly used as traditional medicine in Indonesia. The toxicity of this herb needs to be studied to improve its application. This study aimed to evaluate the toxicity of the crude ethanol extract of temu putih rhizome against brine shrimp (Artemia salina) larvae and zebrafish (Danio rerio) embryos and to analyze the potential active compounds contained in the extract. The crude ethanol extract showed a 50% lethal concentration value (LC50) at 588 ppm against brine shrimp larvae and 224 ppm against zebrafish embryos. Based on the gas chromatography-mass spectrometry analysis, the suspected active compounds that play a role in the toxicity were epicurzerenone, curzerene, and curzerenone, while 2,4,6-trimethylacetophenone was the predominant compound.
Effect of moringa seed (Moringa oleifera Lam.) infusion on stamina performance in male mice Husna, Hanifati; Andriyanto, Andriyanto; Manalu, Wasmen; Mohamad, Kusdiantoro
Current Biomedicine Vol. 3 No. 2 (2025): July
Publisher : School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/currbiomed.3.2.67

Abstract

Background Individuals experience varying levels of fatigue and stamina. Moringa oleifera Lam. Seed extract relieves fatigue due to its high levels of antioxidants and glucosinolate glucomoringin, which reduces intracellular oxidative stress. Objectives This study aimed to determine the effectiveness of infusion of M. oleifera seed as a stamina stimulator in mice. Methods This experiment used 25 male mice divided into five groups (five mice in each group), consisting of M. oleifera seed infusion at doses of 0, 1, 3, and 5 g/kg BW as the negative control and treatment groups, and caffeine at a dose of 6.5 mg/kg BW as the positive control group. Stamina effectiveness was evaluated using a swimming endurance test that measured swimming duration and physiological parameters every 15 min for 2 h after administration. Data were analyzed using analysis of variance (ANOVA) followed by Tukey’s test. Results M. oleifera seed infusion at a dose of 3 g/kg BW had the shortest swimming duration difference (Δ) value, with no difference compared to the 5 g/kg BW and caffeine groups, but was significantly different (P<0.05) compared to the 0 and 1 g/kg BW groups. M. oleifera seed infusion at a dose of 3 g/kg BW also showed a lower delta value for body surface temperature and heartbeat rate compared to the negative control, and a lower delta value for the respiratory rate compared to the 5 g/kg BW group. Conclusion M. oleifera seed infusion potentially maintains stamina performance in mice at a dose of 3 g/kg BW.
The Expression of Type III Collagen Connective Tissue inGluteobiceps and Psoas Mayor Muscles of Aceh Cattle Tanjung, Fitri Aisah; Ali, Ihfan Khaidir; Jalaluddin, Muhammad; Wahyuni, Sri; Sabri, Mustafa; Hidayat, Taufan; Wardhani, Lailia Dwi Kusuma; Ritonga, Mudhita Zikrullah; Rahmi, Erdiansyah; Prasetyaningtyas, Wahono Esthi; Mohamad, Kusdiantoro; Sofyan, Hamny
Acta VETERINARIA Indonesiana Vol. 13 No. 2 (2025): Juli 2025
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.13.2.112-118

Abstract

Collagen is one of the determinant factors of beef tenderness. This study analyzed the expression and content of type III collagen connective tissue in two muscle types of Aceh cattle through immunohistochemical staining. Meat samples were obtained from the gluteobiceps and psoas major muscles of three Aceh cattle bulls weighing 250-300 kg, with a body condition score of three. Histology preparations were created from samples and subjected to immunohistochemical staining utilizing specific antibodies targeting type III collagen. Qualitative data were analyzed descriptively, while quantitative data were analyzed using IMAGE J software. The results showed that the expression of type III collagen connective tissue was found in the endomysium and perimysium layers of both muscles. The expression of type III collagen connective tissue varied significantly among the muscles (P<0.05). The gluteobiceps muscle had an average percentage of the area containing type III collagen connective tissue of 4.47±2.41%, while the psoas major muscle was lower at 2.51±1.69%. This study concludes that the gluteobiceps muscle of Aceh cattle has a higher amount of type III collagen connective tissue than the psoas major muscle.
Efek Campuran Ekstrak Temulawak (Curcuma xanthorrhiza) dan Temuputih (Curcuma zedoaria) terhadap Toksisitas Akut pada Embrio Ikan Zebra Mohamad, Kusdiantoro; Aryani, Nursela Sofyanti Mirza; Prasetyaningtyas, Wahono Esthi; Syahbirin, Gustini
Jurnal Sain Veteriner Vol 42, No 2 (2024): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.94142

Abstract

Temulawak (Curcuma xanthorrhiza) dan temuputih (Curcuma zedoaria) termasuk tanaman herbal yang mempunyai potensi sebagai antitumor dan antikanker. Beberapa obat antikanker dan ekstrak herbal telah dilaporkan memiliki toksisitas yang tinggi dan menyebabkan malformasi pada embrio ikan zebra (Danio rerio). Penelitian ini bertujuan membandingkan toksisitas ekstrak etanol C. xanthorrhiza, C. zedoaria, dan campurannya (rasio 1:6) terhadap embrio ikan zebra. Rimpang C. xanthorrhiza dan C. zedoaria diekstrak dengan cara maserasi dalam etanol 80%. Ekstrak diuji toksisitas akut dengan embrio ikan zebra menggunakan metode OECD Guidelines for the Testing of Chemicals No. 236 (2013). Nilai lethal concentration 50 (LC50) campuran ekstrak adalah 82.9 ppm, berada di antara nilai LC50 masing-masing ekstrak (16.9 untuk C. xanthorrhiza dan 112.1 untuk C. zedoaria). Tingkat menetas embrio rendah pada ekstrak C. xanthorrhiza tetapi tinggi pada ekstrak C. zedoaria dan ekstrak campuran. Kelainan notokorda dan edema kantong kuning telur tinggi pada ekstrak C. xanthorrhiza tetapi rendah pada ekstrak C. zedoaria dan ekstrak campuran. Kelainan sirkulasi darah rendah pada ekstrak C. zedoaria tetapi tinggi pada ekstrak campuran. Edema perikardium dan koagulasi darah merupakan malformasi mayor pada semua ekstrak. Dapat disimpulkan bahwa campuran ekstrak etanol temulawak dan temuputih menyebabkan malformasi edema perikardium, koagulasi darah, dan secara sinergis menghambat sirkulasi darah, yang berkaitan dengan potensi antikanker. Selain itu, ekstrak campuran dapat menurunkan hambatan menetas, malformasi notokorda, dan edema kantong kuning telur.
Effects of Physalis angulata Leaf Extract on Female Reproductive Organs Following Busulfan Injection in Rats Satyaningtijas, Aryani; Ekastuti, Damiana Rita; Tarigan, Ronald; Prasetyaningtyas, Wahono Esthi; Adnyane, I Ketut Mudite; Mohamad, Kusdiantoro; Rahmadhani, Elsi; Sulistyana, Yanti; Mahendra, Arindina
HAYATI Journal of Biosciences Vol. 33 No. 2 (2026): March 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.2.490-498

Abstract

Physalis angulata L. (ciplukan) is traditionally used as an herbal remedy with anticancer and antioxidant properties. At the same time, busulfan, a chemotherapy alkylating agent, causes gonadotoxicity and oxidative stress that impair female reproduction. This study aimed to evaluate the effect of P. angulata on reproductive function after busulfan administration in female rats. Twenty-five 6-week-old female rats were randomly divided into: Group I (control), Group II (busulfan only), Group III (P. angulata only), Group IV (busulfan followed by P. angulata after 14 days), and Group V (busulfan and P. angulata administered for 28 days). FSHR and LHR expression in the ovaries and MDA levels in the ovaries and uterus were measured to assess reproductive changes. Busulfan showed no significant effect on FSHR and LHR, whereas P. angulata induced downregulation, suggesting a potential negative feedback mechanism on ovarian receptors. Ovarian MDA showed a decreasing trend with combined treatments, while uterine MDA peaked after busulfan but declined markedly with P. angulata, indicating its role in alleviating oxidative stress. In conclusion, P. angulata may modulate hormonal balance in rat ovaries and decrease oxidative stress in the uterus after busulfan treatment.
Kultur Tiga Dimensi Testis Tikus (Mus musculus) dalam Berbagai Media: Kajian Morfologi Wahono Esthi Prasetyaningtyas; Nelza Putri Yasmin; Ronald Tarigan; Kusdiantoro Mohamad; Mokhamad Fahrudin
Jurnal Veteriner Vol. 26 No. 3 (2025)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2025.26.3.311

Abstract

Kultur sel merupakan proses isolasi sel dari jaringan hewan dan menumbuhkannya dalam lingkungan buatan yang terkontrol. Terdapat dua metode utama dalam kultur sel, yaitu kultur dua dimensi (2D) dan kultur tiga dimensi (3D). Penelitian ini bertujuan untuk mengidentifikasi medium paling efektif untuk kultur jaringan testis secara tiga dimensi. Medium yang diuji meliputi Alpha Modified Eagle Medium (αMEM), Dulbecco's Modified Eagle Medium (DMEM), Knockout® Serum Replacement (KSR), dan Fetal Bovine Serum (FBS). Potongan kecil testis mencit dewasa dikultur dalam tiga kombinasi media yang berbeda: αMEM dengan 10% FBS, αMEM dengan 10% KSR, dan DMEM dengan 10% KSR, selama tujuh dan 14 hari pada suhu 35 °C dalam atmosfer yang mengandung 5% karbon dioksida (CO₂). Setelah masa kultur, jaringan diproses untuk pemeriksaan histologis dan diwarnai menggunakan pewarnaan hematoksilin dan eosin (HE). Hasil penelitian menunjukkan bahwa jumlah sel spermatogonia relatif sama pada semua media baik pada hari ke-7 maupun hari ke-14. Namun, pada hari ke-7, jumlah spermatosit primer secara signifikan lebih tinggi pada kelompok αMEM + 10% KSR dibandingkan dengan αMEM + 10% FBS, dan juga lebih tinggi dibandingkan dengan DMEM + 10% KSR. Pada hari ke-14, tidak ditemukan perbedaan signifikan dalam jumlah spermatosit primer antar tiga kelompok. Mengenai sel spermatid, pada hari ke-7, DMEM + 10% KSR menghasilkan jumlah sel yang secara signifikan lebih tinggi dibandingkan dua media lainnya. Pola ini berlanjut hingga hari ke-14, dan DMEM + 10% KSR tetap menunjukkan jumlah sel spermatid tertinggi. Secara keseluruhan, kombinasi media DMEM + 10% KSR ditemukan sebagai medium paling efektif untuk mendukung kultur jaringan testis secara tiga dimensi.
HEPATOPROTECTIVE EFFECT OF Physalis angulata L. LEAF ETHANOL EXTRACT AGAINST BUSULFAN-INDUCED LIVER DAMAGE IN FEMALE RATS Elsi Rahmadhani; Aryani Sismin Satyaningtijas; Damiana Rita Ekastuti; Ronald Tarigan; I Ketut Mudite Adnyane; Kusdiantoro Mohamad; Wahono Esthi Prasetyaningtyas; Yanti Sulistyana
BIOTROPIA Vol. 33 No. 3 (2026): BIOTROPIA Vol. 33 No. 3 September 2026
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2026.33.3.2588

Abstract

ARTICLE HIGLIGHTSBusulfan can damage the liver by triggering oxidative stress Physalis angulata leaves show natural liver protective effects Extract reduced oxidative damage in liver tissue Liver structure improved after extract treatment Early treatment provided stronger liver protection than delayed useABSTRACTLiver disease is the leading cause of death worldwide, with drug-induced hepatotoxicity representing a major contributing factor. Busulfan, an anticancer drug used in chronic myeloid leukemia, is cytotoxic to proliferating cells and can induce liver injury. Physalis angulata L. (ground cherry) contains bioactive compounds with potential hepatoprotective properties. This study aimed to evaluate the protective effects of ethanol extract of Phyasalis angulata L. (EEPA) leaves on busulfan-induced liver damage in female Sprague–Dawley rats. Twenty-five rats were randomly divided into five groups: P1 (normal control), P2 (busulfan 40 mg/kg BW, single dose), P3 (extract 300 mg/kg BW, days 1–28), P4 (busulfan + extract, days 1–28), and P5 (busulfan + extract, days 14–28). Parameters assessed were alanine aminotransferase (ALT) and aspartate aminotransferase (AST), the level of malondialdehyde (MDA) in liver, and liver histology. Data were analyzed using one-way analysis of variance (ANOVA) followed by a post hoc test. The results showed that Physalis angulata L. extract did not cause significant differences in the serum ALT and AST levels but significantly reduced the MDA concentrations and improved the liver histological architecture. Busulfan administration significantly decreased hepatocyte count and increased Kupffer and inflammatory cells, fatty degeneration, and necrosis. Early administration of the extract (P4) demonstrated stronger hepatoprotective effects compared with delayed administration (P5). These findings suggest that EEPA leaves exerts hepatoprotective effects against busulfan-induced liver injury in rats, likely through antioxidant activity, as evidenced by subsequent histological improvement, and highlighting its potential as a natural hepatoprotective agent.
Short-term treatment of osteoarthritis-induced sheep with human umbilical cord–derived mesenchymal stem cells: A morphological and proteomic analysis Kusdiantoro Mohamad; Adrian Situmeang; Handina Rakhmawati; I Ketut Mudite Adnyane; Mokhamad Fahrudin; Nurhidayat Nurhidayat; Harry Murti; Andri Maruli Tua Lubis; Arief Boediono
Jurnal Medik Veteriner Vol. 9 No. 1 (2026): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol9.iss1.2026.106-123

Abstract

Knee joints are the primary sites affected by osteoarthritis (OA) in humans and animals. Although intra-articular human umbilical cord–derived mesenchymal stem cells (hUC-MSCs) show therapeutic promise, their combined use with hyaluronic acid (HA) remains underexplored in large animals. This study evaluated morphological and proteomic changes in a sheep osteoarthritis model treated with both agents. A total of twelve sheep were divided into four groups (n = 3 per group): control (normal and OA) and three treatment groups receiving hUC-MSCs, HA, or a combination of HA and hUC-MSCs (HA + hUC-MSCs). OA was induced by meniscectomy, and intra-articular injections were administered four weeks post-surgery. Macroscopic scoring was conducted according to the Osteoarthritis Research Society International system, while histological evaluation was performed using hematoxylin–eosin, Safranin O, and Masson’s trichrome staining. Proteomic analysis of synovial fluid was carried out using liquid chromatography–tandem mass spectrometry. The macroscopic OA score in the HA + hUC-MSC group was lower than in the other treatment groups and was not significantly different from the normal group (p > 0.05). Microscopic observations indicated that hUC-MSCs supported joint cartilage regeneration by preserving proteoglycan and collagen content. Proteomic profiling revealed that treatment with HA + hUC-MSCs modulated proteins associated with complement activation and coagulation pathways. The combination of HA and hUC-MSCs provided synergistic benefits for cartilage regeneration in a sheep OA model during a short treatment period. These findings suggest that this approach represents a promising regenerative therapy for OA in large animals within veterinary medicine and has translational relevance for human regenerative medicine.
Corpus alienum pada saluran pencernaan kura-kura baning (Manouria emys) di Kebun Binatang Bandung Monica Silva Jerica; Josephin Bernadette; Teresa Wening Anggitasari; Noval Ekarian Ramadan; Kusdiantoro Mohamad
ARSHI Veterinary Letters Vol. 9 No. 2 (2025): ARSHI Veterinary Letters - May 2025
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.9.2.53-54

Abstract

Kura-kura memiliki kebiasaan menelan benda asing yang dikenal sebagai geofagi. Benda asing yang tertelan dapat menimbulkan obstruksi pada saluran pencernaan. Seekor kura-kura baning cokelat (Manouria emys), jenis kelamin jantan, dengan berat badan 13,5 kg, dilaporkan mengalami penurunan nafsu makan, lemas, tidak dapat defekasi, dan mata berair. Pemeriksaan fisik dan pemeriksaan penunjang radiografi (X-ray) menunjukkan akumulasi benda asing di saluran pencernaan. Penanganan yang dilakukan berupa pemasangan feeding tube, pemberian gliserin, dan pemberian biodin. Setelah penanganan, kura-kura mengeluarkan benda asing berupa pasir saat defekasi dan selanjutnya mengalami peningkatan kondisi tubuh. Kura-kura aktif kembali dan dinyatakan sembuh setelah 18 hari penanganan.
Neurogical deficit score dan gambaran hematologi tikus model strok iskemik dengan metode middle cerebral artery occlusion Ika Satya Perdhana; Muhamad Evans Hardiansyah; Hera Maheshwari; Kusdiantoro Mohamad
ARSHI Veterinary Letters Vol. 9 No. 3 (2025): ARSHI Veterinary Letters - August 2025
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.9.3.83-84

Abstract

Stroke is a neurological disorder caused by impaired blood flow to specific regions of the brain, often resulting in paralysis and functional deficits in the affected area. Among its subtypes, ischemic stroke is the most prevalent in the general population. This study aimed to establish a rat model of ischemic stroke induced by middle cerebral artery occlusion (MCAO). Eight male Sprague-Dawley rats aged 8–10 weeks were randomly assigned to two groups: control and treatment group. In the treatment group, the middle cerebral artery was occluded for 45 min. Neurological assessment using the Bederson scale revealed a neurological deficit score (NDS) of 1, characterized by contralateral flexion after left-brain injury. Hematological analysis demonstrated significantly higher white blood cell (WBC) counts (p < 0.05), whereas red blood cell (RBC) counts, hemoglobin concentration, and hematocrit values were significantly lower (p < 0.05) than those in the control group. These findings indicate that the MCAO method with a 45-minute occlusion successfully induced a mild neurological deficit accompanied by distinct hematological alterations, thereby providing a reproducible animal model for ischemic stroke research.
Co-Authors . NURBARIAH A.S. Satyaningtijas Aditianingrum, Kurnia Alysia Adrian Situmeang Ali, Ihfan Khaidir Andri Maruli Tua Lubis Andriyanto Andriyanto Andriyanto Andriyanto Anggara Widyawiratma Aprilia Hardiati Arief Boediono Aryani Satyaningtijas Aryani, Nursela Sofyanti Mirza Bambang Purwantara Damiana Rita Ekastuti Damiana Rita Ekastuti Deny Putra Romadhon Edwar, Syafri Ekayanti Mulyawati Kaiin Elsi Rahmadhani Erdiansyah Rahmi Faisal Anthony Faisal Mustafa Faralinda Sari Fitri Susana Gatot Prasojo Gustini Syahbirin Hamny Sofyan Handina Rakhmawati Harry Murti Hera Maheshwari Husna, Hanifati Ika Satya Perdhana Iman Supriatna Ita Djuwita Ita Djuwita ITA DJUWITA ITA DJUWITA Ita Djuwita Josephin Bernadette Kaiin, Ekayanti Mulyawati Ketut Adnyane Mudite Lina Noviyanti Sutardi M Agus Setiadi Mahendra, Arindina Maria Yohana Simarmata Min Rahminiwati Mokhamad Fahrudin Monica Silva Jerica Muhamad Evans Hardiansyah Muhammad Jalaluddin, Muhammad Muhammad Joserisal Alghifarry Pakkawaru Muhammad Naufal Febrian Jamaludin Nelza Putri Yasmin Ni Wayan Kurniani Karja Noval Ekarian Ramadan Nurhidayat . Nurhidayat Nurhidayat Pardede, Friselsa R. Iis Arifiantini Rahardi, Nindya Anandaputri Rahmadhani, Elsi Rahmaniyah, Wiwit Ridhani Rantimah . Raudhatul Jannah Ritonga, Mudhita Zikrullah Ronald Tarigan Siti Rabi’ah SM Leluala Sri Wahyuni Srihadi Agungpriyono Subangkit, Mawar Sulistyana, Yanti Syafri Edwar Tanjung, Fitri Aisah Tarigan, Ronald Taufan Hidayat Teresa Wening Anggitasari TUTY LASWARDI YUSUF Tuty Laswardi Yusuf Utami Idha Pangesti Vincentia Maria Vivit Riyacumala Wahono Esthi Prasetyaningtijas Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wahono Esthi Prasetyaningtyas Wardhani, Lailia Dwi Kusuma Wasmen Manalu Wiwit Ridhani Rahmaniyah Yanti Sulistyana Yohan Rusiyanto Yuhara Sukra