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Uji Lapangan Formula Cair Pseudomonas fluorescens P60 terhadap Layu Fusarium pada Tanaman Tomat Loekas Soesanto; Endang Mugiastuti; Ruth Feti Rahayuniati; Abdul Manan
Jurnal Perlindungan Tanaman Indonesia Vol 17, No 2 (2011)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3043.642 KB) | DOI: 10.22146/jpti.9830

Abstract

A research aimed at knowing 1) the effect of Pseudomonas fluorescens P60 in liquid formula on Fusarium wilt of tomato, 2) the effect of P. fluorescens P60 in the formula on tomato growth and yield, and 3) P. fluorescens P60 mechanisms on tomata was carried out at tomato field of Selomoyo Village, Kaliangkrik Subdistrict, Magelang Regency at altitude of 826 m above sea level. Randomized block design was used with seven treatments and four replicates. The treatments were control, with P. fluorescens P60 soaked for 15 min and without fungicide, pathogen without P. fluorescens P60 with fungicide (PBG1), pathogen with P. fluorescens P60 without fungicide, pathogen with pouring P. fluorescens P60 1, 3, and 5 times. Result indicated that application of formulated P. fluorescens P60 for 5 times decreased the disease intensity as high as 26.77%, and late population of the pathogen but increased P. fluorescens P60 as high as 4.54×1010 cfu ml-1. P. fluorescens P60 affected growth and yield of tomato. P. Fluorescens P60 induced tomato resistance by increasing qualitatively its phenolic compound content (saponin, tannin, glycoside). Penelitian dengan tujuan untuk mengetahui: 1) pengaruh Pseudomonas fluorescens P60 dalam formula cair terhadap penyakit layu fusarium pada tanaman tomat, 2) pengaruh P. fluorescens P60 dalam formula cair terhadap pertumbuhan dan produksi tanaman tomat, dan 3) mekanisme P. fluorescens P60 pada tanaman tomat dilakukan di lahan Desa Selomoyo, Kecamatan Kaliangkrik, Kabupaten Magelang dengan ketinggian 826 m di atas permukaan laut. Penelitian menggunakan Rancangan Acak Kelompok (RAK), dengan 7 perlakuan dan jumlah ulangan 4 kali, dan setiap unit terdiri atas 8 tanaman. Perlakuan tersebut meliputi kontrol; dengan P. fluorescens P60 rendam 15 menit dan tanpa fungisida; dengan patogen; tanpa P. fluorescens P60; dengan fungisida (PBG1); patogen, tanpa P. fluorescens P60, tanpa fungisida; patogen, dengan penyiraman P. fluorescens P60 1 kali; patogen, dengan penyiraman P. fluorescens P60 3 kali; dan patogen, dengan penyiraman P. fluorescens P60 5 kali. Pemberian P. fluorescens P60 selama 5 kali memberikan pengaruh sangat nyata dalam menekan penyakit layu fusarium yang disebabkan Fusarium oxysporum. Hal ini ditunjukkan pada penurunan intensitas penyakit sebesar 26,77%, rendahnya kepadatan akhir F. oxysporum serta tingginya nilai kepadatan P. fluorescens P60 sebesar 4,54×1010 unit pembentuk spora/ml. Pengaruh pemberian P. fluorescens P60 belum menunjukkan pengaruh nyata pada komponen pertumbuhan dan hasil. P. fluorescens P60 mampu mengimbas ketahanan tanaman tomat dengan meningkatkan kandungan senyawa fenol (saponin, tanin, glikosida).
Pemanfaatan Enzyme-Linked Immunosorbent Assay untuk Mengukur Titer Virus dalam Bawang Putih Susamto Somowiyarjo; Endang Mugiastuti; Y. M. Sugi Maryudani
Jurnal Perlindungan Tanaman Indonesia Vol 6, No 1 (2000)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12407

Abstract

Non-precoated Indirect ELISA had been developed by employing monoclonal antibodies against virus isolated from Sangga variety of garlic. The ELISA was used to measure the titer of virus in the plant. In comparation with biological assay using Chenopodium amaranticolor, ELISA was able to measure the virus titer faster and was more simpler. The highest titer of virus was obtained using the first leaf of garlic at age of 29-36 days after planting. Application of nitrogen at high dose and high temperature of garlic cultivation trends to increase the virus titer. The results of this experiment may be used to improve the method of sampling to detect virus in garlic tissues.
Aplikasi metabolit sekunder dari tiga isolat Pseudomonas fluorescens untuk mengendalikan penyakit antraknosa pada daun kakao Fitrianti FITRIANTI; Loekas - SOESANTO; Endang MUGIASTUTI; Murti Wisnu Ragil SASTYAWAN; Abdul MANAN
E-Journal Menara Perkebunan Vol 90, No 1 (2022): April, 2022
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v90i1.483

Abstract

Anthracnose caused by Colletotrichum gloeosporoides is an important disease in cocoa. This research aimed to determine the effectiveness of secondary metabolites derived from three isolates of Pseudomonas fluorescens to control cocoa leaves anthracnose, and their influence on the growth of cocoa plants. The research was conducted at a smallholder cocoa plantation in Putat Village, Patuk District, Gunung Kidul Regency, Yogyakarta for four months. A randomized block design was used with four replicates and seven treatments consisted of control, application of secondary metabolites from P. fluorescens P60, P. fluorescens P20, P. fluorescens P8, combination P. fluorescens P60 + P20, P. fluorescens P60 + P8, and P. fluorescens P20 + P8. Variables observed were disease intensity, infection rate, number of healthy shoots and qualitative phenolic compound. Resultsof the research showed that the secondary metabolites of P. fluorescens P60, P20 and P8 alone or in combination  suppressed the disease intensity by42.01-54.50%. The infection rate caused by metabolite secondary of P. fluorescens P60, P20, P8, P. fluorescens P60+P20, P. fluorescens P60+P8,and P. fluorescens P20+P8 was 0.23; 0.25; 0.26; 0.26; 0.31; and 0.24 units/day, respectively. The secondary metabolites of P. fluorescens P60 increased the number of healthy shoots by 67.44 %. The secondary metabolites of P. fluorescens P60 increased phenolic compounds (tannin, saponin, and glycosides) in cocoa leaves.[Keyword: leaves anthracnose, Colletotrichum gloeosporoides, cocoa, secondary metabolites, Pseudomonas fluorescens] AbstrakAntraknosa yang disebabkan oleh Colletotrichum gloeosporioides merupakan penyakit penting pada tanaman kakao. Penelitian bertujuan mengkaji keefektifan metabolit sekunder dari tiga isolat Pseudomonas fluorescens untuk mengendalikan penyakit antraknosa pada daun kakao, serta pengaruhnya terhadap pertumbuhan tanaman kakao. Penelitian dilaksanakan di perkebunan kakao rakyat, Desa Putat, Kecamatan Patuk, Kabupaten Gunung Kidul, Yogyakarta selama empat bulan. Penelitian menggunakan rancangan acak kelompok untuk menguji 7 perlakuan yang diulang 4 kali, perlakuan yang diuji adalah kontrol, aplikasi metabolit sekunder P. fluorescens P60, P. fluorescens P20, P. fluorescens P8, kombinasi P. fluorescens P60+P20, P. fluorescens P60+P8, dan P. fluorescens P20+P8. Variabel pengamatan meliputi intensitas penyakit, laju infeksi, jumlah tunas sehat, dan kandungan senyawa fenol secara kualitatif. Hasil penelitian menunjukkan bahwa perlakuan metabolit sekunder P. fluorescens P60, P20, dan P8 secara tunggal dan gabungan mampu menekan intensitas penyakit berkisar 42,01-54,50%. Laju infeksi perlakuan metabolit sekunder P. fluorescens P60, P. fluorescens P20, P. fluorescens P8, kombinasi P. fluorescens P60+P20, P. fluorescens P60+P8 dan P. fluorescens P20+P8 berturut-turut 0,23; 0,25; 0,26; 0,26; 0,31; dan 0,24 unit/hari. Metabolit sekunder P. fluorescens P60 meningkatkan jumlah tunas sehat sebesar 67,44%. Perlakuan P. fluorescens P60 meningkatkan senyawa fenol (saponin, tanin, dan glikosida) pada daun kakao.[Kata kunci: antraknosa daun, Colletotrichum gloeosporoides, kakao, metabolit sekunder, Pseudomonas fluorescens]
Penerapan Teknologi Pengendalian Penyakit Tanaman Yang Ramah Lingkungan Pada Tembakau Endang Mugiastuti; Loekas Soesanto; Abdul Manan
JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat) VOL. 2 NOMOR 2 SEPTEMBER 2018 JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat)
Publisher : Lembaga Publikasi Ilmiah dan Penerbitan (LPIP)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (908.003 KB) | DOI: 10.30595/jppm.v2i2.1793

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Tobacco is one of the most important plants which were cultivated by Farmers Groups "Darma Mukti Tani I" and "Karya Tani" of Wangon District, Banyumas Regency. However, several obstacles often interfere with the production of tobacco, including quite high tobacco pests and diseases. Socialization of  plant pests diseases management by using Trichoderma sp. and Pseudomonas fluorescens biopesticides, needs to be done. The activity aimed was to increase knowledge and skills of farmers on pests and diseases in tobacco and their management measures with biological pesticides. Issues addressed farmers partners with several technology solution, which was conducted by the transfer of technology through education, training, demonstration plots, and mentoring. Dissemination activities were able to increase their knowledge of pests and diseases, their control, and the control of environmental friendly as 58,82, 62,5, and 67.67%, respectively. The training activities of P. fluorescens exploration was able to improve their ability as 166.67%. Propagation activity and application training could improve their ability for 100 and 73,33%, respectively. The training activities of Trichoderma sp. exploration were able to improve their ability as 94,44%, while the propagation and application training could improve their ability, respectively, as 73,33 and 88.23%. Application of biological pesticides P. fluorescens and Trichoderma sp. at demonstration plot was able to increase growth and reduced the intensity of bacterial and fusarium wilt disease by 40-50%.Keywords: Tobacco, technology transfer, pests and diseases, biological control.
Pelatihan Teknologi Budidaya dan Pengelolaan Penyakit Ramah Lingkungan Pada Tanaman Lada Endang Mugiastuti; Loekas Soesanto; Abdul Manan
JURNAL PENGABDIAN KEPADA MASYARAKAT Vol 8, No 2 (2018): Desember 2018
Publisher : LPPM UNINUS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (235.614 KB) | DOI: 10.30999/jpkm.v8i2.279

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Pepper is an important commodity and has high economic value. This commodity has begun to be cultivated by members of the PKK RT 07 and PKK RT 09, Tambak Sogra Village, Sumbang District, Banyumas Regency, in an effort to increase the use of the yard and increase family income. However, the knowledge and skills of PKK members regarding cultivation and diseases of pepper plants as well as effective and environmentally friendly management efforts are very low. The activity aimed was to improve knowledge and skills of members of the PKK group in the cultivation and management of pepper disease that are effective and environmentally friendly. Activities carried out with dissemination, training, and technology demonstration plots. The activities were able to increase the knowledge and skills of members of the PKK group in pepper cultivation and the management of the disease which is environmentally friendly. In addition, the application of Trichoderma sp. and Pseudomonas fluorescens in the demonstration plot can increase the growth of pepper.
Sosialisasi dan Pelatihan Pemanfaatan Biopestisida Mikroba Antagonis Campuran untuk Mengendalikan Penyakit Bawang Merah di Musim Hujan Abdul Manan; Endang Mugiastuti; Loekas Soesanto
Jurnal Pengabdian Pada Masyarakat Vol 6 No 1 (2021)
Publisher : Universitas Mathla'ul Anwar Banten

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30653/002.202161.630

Abstract

SOCIALIZATION AND TRAINING ON THE UTILIZATION OF MIXED ANTAGONISTIC MICROBIAL BIOPESTICIDES TO CONTROL SHALLOTS IN THE RAINY SEASON. The constraints on shallot cultivation in the rainy season are the more severe attacks of Fusarium oxysporum (Fusarium wilt) and Alternaria porii (purple disease). Solid formula of Trichoderma sp., Bacillus sp. B8, and Bacillus sp. B11 have been proven to increase their effectiveness and also cultivation technique and environmental modification. However, the benefit of the technology did not informed members of Farmers Group in Tambaksogra Village, Sumbang Regency, Banyumas District yet. The objectives of the activity are to increase farmers’ knowledge and skills on shallot cultivation technique out of the season, to multiply and to apply the antagonistic microbes solid mixed formula, to increase the crop production quantity and quality, and to increase farmers’ income. The method used was transfer of the technology through education, training, and demplot. The activities carried out were socialization of the potency and application of shallot cultivation technique out the season, multiplication and application of the antagonistic microbial solid mixed formula with the use of available media at the location, demonstration plot of the shallot cultivation technology with the application of the solid mixed formulation compared to single antagonistic, and farmers’ accompaniment in implementing the technology package. Result showed that there was an increase in farmers’ knowledge on the potency and application of solid formula biopesticides by 69.61%, as well as an increase skill in making solid formula biopesticide and its application by 64.33%.
Viabilitas dan Virulensi Tujuh Belas Tahun Penyimpanan Fusarium oxysporum Schlecht. f.sp. zingiberi Trujillo Dalam Tanah Steril Elvina Hapsari; Loekas Soesanto; Endang Mugiastuti
Media Pertanian Vol 6, No 2 (2021): Media Pertanian
Publisher : Program Studi Agroteknologi Universitas Siliwangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37058/mp.v6i2.3823

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Koleksi dan penyimpanan mikroba diperlukan untuk penelitian lebih lanjut, sumber biodiversitas, koleksi, dan konservasi plasma nutfah mikroba. Salah satu teknik penyimpanan mikroba adalah dalam tanah steril. Penelitian ini bertujuan untuk mengetahui viabilitas dan virulensi beberapa isolat Fusarium oxysporum f.sp. zingiberi setelah disimpan selama tujuh belas tahun pada tanah steril. Rancangan yang digunakan adalah Rancangan Acak Lengkap dengan tiga ulangan dan 17 perlakuan, yaitu isolat F. oxysporum f.sp. zingiberi asal Magelang terdiri atas MBO1, MBO2, MSO1, MSO3, MSO4, MCO1, MCO2, MBM, MSH dan MSS, serta asal Purworejo, yaitu PBO1, PBO2, PPO1, PKO2, PKO3, PPH dan PBOSi. Variabel yang diamati adalah warna koloni, diameter koloni, mikrokonidium, makrokonidium, waktu pertumbuhan, kecepatan tumbuh, berat kering miselium, kerapatan konidium, masa inkubasi, luas serangan, selisih bobot, dan indeks sampah. Hasil penelitian menunjukkan bahwa semua isolat F. oxysporum f.sp. zingiberi asal Magelang dan Purworejo, yang telah disimpan dalam medium tanah steril selama tujuh belas tahun, masih tumbuh dengan baik pada medium PDA dan memenuhi cawan Petri pada umur 6-19 hari. Isolat F. oxysporum f.sp. zingiberi tersebut masih mampu menimbulkan gejala busuk rimpang jahe dengan masa inkubasi 11-21 hari setelah inokulasi. Rerata luas serangannya antara 2,07-2,65 mm2. Rerata luas serangan terbesar dan terkecil terdapat pada perlakuan PBOSi dan MSO4 masing-masing sebesar 2,65 dan 2,07 mm2 atau 49,00 dan 16,28 % dibandingkan kontrol Rerata indeks sampah tertinggi dan terendah masing-masing terdapat pada PBO2 dan MCO1 sebesar 39,56 dan 18,70 g atau 129,00 dan 8,4 % dibandingkan kontrol.
Pemanfaatan Macam dan Dosis Pupuk untuk Meningkatkan Pertumbuhan dan Hasil Jambu Biji Kristal (Psidium Guajava) Etik Wukir Tini; Anang Khairul Rahman; Endang Mugiastuti
Agrotechnology Research Journal Vol 3, No 1 (2019): Agrotechnology Research Journal
Publisher : Perkumpulan Agroteknologi/Agroekoteknologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (484.056 KB) | DOI: 10.20961/agrotechresj.v3i1.30408

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Fertilization is any effort that aims to increase the availability of nutrients needed by plants, and increase crop production. The study aimed to obtain fertilizer types, fertilizer dosages and combinations of types and dosages of fertilizers suitable for growth and yield of Crystal Guava plants. The study was conducted on October 2018 to Mei 2019 on farmer’s land, Banjarsari Kulon Village, Sumbang District, Banyumas Regency and Agronomy and Horticulture Laboratory of the Faculty of Agriculture, Jenderal Sudirman University. The experimental design used was a Randomized Completely Block Design (RCBD) with 2 factors and 3 replications. The first factor was the type of fertilizer, namely NPK, SP-36 and MKP fertilizers. The second factor was fertilizer dosage, namely 20 g / plant, 30 g / plant, 40 g / plant and 50 g / plant. Observed data were done by Analysis of Variance and if it significantly affected then DMRT (Duncan’s Multiple Range Test) was carried out at the level of 5%. The results showed that the most effective type of NPK fertilizer and dosage of 20 g / plant. The combination of NPK fertilizer with a dose of 20 g / plant was the most effective treatment for increasing the growth and yields of the Crystal Guava plant.
Pengelolaan Tanaman Bawang Merah Ramah Lingkungan dengan Pemanfaatan Biopestisida Trichoderma Abdul Manan; Nurtiati Nurtiati; Endang Mugiastuti
Jurnal SOLMA Vol. 7 No. 2 (2018)
Publisher : Universitas Muhammadiyah Prof. DR. Hamka (UHAMKA Press)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29405/solma.v7i2.2160

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Kelompok tani "Rukun Tani I” Desa Sumbang , Kecamatan Sumbang Kabupaten Banyumas Jawa Tengah merupakan kelompok tani yang banyak mengusahakan tanaman hortikultur. Permasalahan utama yang dihadapi dalam peningkatan produksi bawang merah adalah tingginya serangan penyakit moler (Fusarium oxysporum) dan hama ulat bawang (Spodoptera exigua). Sosialisasi pengelolaan hama dan penyakit bawang merah dengan menggunakan biopestisida Trichoderma sp. perlu dilakukan. Tujuan kegiatan adalah meningkatkan pengetahuan dan keterampilan petani tentang hama dan penyakit bawang merah serta tindakan pengelolaannya dengan pestisida hayati berbasis Trichoderma. Permasalahan mitra diatasi dengan beberapa pemecahan teknologi, yang dilakukan dengan metode transfer teknologi melalui pendidikan, pelatihan, demplot, dan pendampingan. Kegiatan sosialisasi mampu meningkatkan pengetahuan petani tentang jenis hama dan penyakit bawang merah, pengendaliannya, dan pengendalian yang ramah lingkungan sebesar 72,98%. Sedangkan. kegiatan pelatihan perbanyakan dan aplikasi Trichoderma mampu meningkatkan kemampuan petani masing-masing sebesar 48,57 % dan 34,09 %. Aplikasi pestisida hayati Trichoderma sp. pada petak demplot mampu meningkatkan pertumbuhan dan hasil bawang merah sebesar 7,09 % dan menurunkan intensitas hama dan penyakit sebesar 34,16 %.
Raw Secondary Metabolites of Trichoderma harzianum T10 in Tapioca Flour Towards Cucumber Damping-off Loekas Soesanto; Hidayatul Ilahiyyah; Endang Mugiastuti; Abdul Manan; Rostaman Rostaman
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 2 (2020): August 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i2.24567

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Trichoderma harzianum is effective for controlling soil-borne pathogenic fungi and producing secondary metabolites. When applied in the field, the raw secondary metabolites are quickly decreased directly by sunlight. One strategy to avoid degradation is the use of tapioca fluor liquid formula for biological control agents. This research aimed to obtain the most effective concentration of tapioca flour in development of raw secondary metabolites of Trichoderma harzianum T10, its effect on damping-off and growth of cucumber. This research was carried out at the screen house and the Plant Protection Laboratory, Faculty of Agriculture, Jenderal Soedirman University from September 2017 up to January 2018. The study was conducted in two stages, i.e., in vitro and in planta. The in vitro stage used completely randomized design with five repetitions and five treatments consisted of T. harzianum T10 in Potato Dextrose Broth, and in 0.5; 1; 1.5; and 2% of tapioca flour media. In in planta, randomized block design was used with five repetitions and six treatments consisted of control, T. harzianum T10 in PDB, and in 0.5; 1; 1.5, and 2% of tapioca flour media. Variables observed were density of conidia, disease incubation period, disease incidence, AUDPC, maximum growth potential, germination ability, plant height, canopy fresh weight, root length, and fresh root weight. Result of the research showed that the highest conidial density (1.23 x 107 conidia mL-1) of T. harzianum T10 was found in 2% tapioca flour with an increase of 63.28% compared to the PDB. The tapioca flour of 1 and 2%, and PDB could suppress the disease incidence by 81.82%. The lowest AUDPC was at 2% tapioca flour. The raw secondary metabolites could not delay the incubation period significantly and increase cucumber plant growth. The novelty is the use of antagonistic fungi in terms of raw secondary metabolites and the discovery of tapioca flour with the right concentration to produce high conidia density and high raw secondary metabolites. The benefits are to find other cheaper ingredients in promoting antagonistic fungal growth and the use of antagonistic fungal bioactive compounds to control plant pathogen