Claim Missing Document
Check
Articles

UJI EMPAT ISOLAT Trichoderma harzianum PADA PENGOMPOSAN KOTORAN SAPI DAN AYAM DAN PENGARUHNYA TERHADAP PERTUMBUHAN MENTIMUN IN PLANTA Oktafiyanto, Muhammad Firdaus; Soesanto, Loekas; Mugiastuti, Endang; Rahayuniati, Ruth Feti; Tamad, Tamad
Agro Bali : Agricultural Journal Vol 3, No 1 (2020)
Publisher : Universitas Panji Sakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (593.796 KB)

Abstract

Penelitian bertujuan: 1) mengetahui pengaruh empat isolat Trichoderma harzianum dalam pengomposan kotoran (ayam dan sapi), 2) interaksi T. harzianum dan kotoran terhadap pertumbuhan, dan 3) kompos terbaik untuk tanaman mentimun. Rancangan Acak Kelompok digunakan dengan 10 perlakuan dan 3 ulangan. Perlakuan terdiri atas kotoran ayam dan sapi, T. harzianum. T10, T213, T14, dan T16.. Variabel yang diamati pH, C/N rasio, suhu, warna kompos, kepadatan dan kegigasan konidium, komponen pertumbuhan, analis jaringan tanaman, dan serapan N tanaman. T. harzianum T10 dan T14 mampu menurunkan C/N rasio pada kotoran sapi. T. harzianum terbaik adalah T14 pada kotoran sapi yang memiliki nilai tertinggi pada panjang tanaman sebesar 76 cm, panjang akar 22 cm, dan berat basah tajuk 14,96 g. T. harzianum T10 pada kotoran sapi memiiki nilai tertinggi pada berat tanaman segar 17,86 g, bobot basah akar 3,04 g, dan jumlah daun 8,88 helai.DOI: 10.37637/ab.v3i1.424
Potential of Various Rice-Washing Water as a Source of Manufacturing Secondary Metabolites of Trichoderma harzianum T10 to Control Cucumber Crown and Root Rot Soesanto, Loekas; Dapawole, Michelia Alba; Sastyawan, Murti Wisnu Ragil; Mugiastuti, Endang; Manan, Abdul; Istiqomah, Dina
Agrotechnology Research Journal Vol 8, No 2 (2024): Agrotechnology Research Jurnal
Publisher : Perkumpulan Agroteknologi/Agroekoteknologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agrotechresj.v8i2.92490

Abstract

Cucumber is one of the most widely consumed vegetable crops globally. Rice-washing water has not been previously explored as a potential source for producing secondary metabolites from antagonistic fungi. Phytophthora species frequently affect cucumber seedlings, leading to crown and root rot. This research aimed to assess the efficacy of washing water from various rice types on the conidial density of Trichoderma harzianum T10 and its impact on crown and root rot in cucumber seedlings and overall plant growth. We conducted four treatments with rice washing water in vitro under a completely randomized design, comprising six replicates. Five treatments were evaluated in planta under a randomized block design with five replicates. The observed variables included conidial density, incubation period, disease incidence, disease progression expressed as the Area Under the Disease Progress Curve (AUDPC), plant height, fresh weight, and root length. The results indicated that washing water from glutinous rice provided the optimal medium for T. harzianum T10, yielding a conidial density of 10.3 × 10-6 conidia mL-1, representing a 66.02% increase compared to washing water from white rice. The crude secondary metabolites produced by T. harzianum T10 in glutinous rice washing water significantly extended the incubation period and reduced disease incidence and AUDPC values by 40.34, 62.07, and 69.41%, respectively, compared to the control. Furthermore, the secondary metabolites from T. harzianum T10 in glutinous rice washing water enhanced plant height, fresh weight, and root length by 91.81, 92.42, and 95.21%, respectively, compared to the control.
Iron Contaminated Soils Remediation Using Secondary Metabolites of Trichoderma harzianum T10 and Its Effect on Spinach Growth Soesanto, Loekas; Sastyawan, Murti Wisnu Ragil; Mugiastuti, Endang; Tamad, Tamad
Agrotechnology Research Journal Vol 7, No 2 (2023): Agrotechnology Research Jurnal
Publisher : Perkumpulan Agroteknologi/Agroekoteknologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/agrotechresj.v7i2.79946

Abstract

Iron (Fe) is an essential metal whose presence in excess can pollute the environment, cause toxic effects on plants, and degrade soil quality. Efforts have been made to overcome this by remediation using secondary metabolites of Trichoderma harzianum T10. This study aimed to determine the potency and appropriate concentration of T. harzianum T10 secondary metabolites as a remediator for Fe-contaminated soil and its effect on the growth and yield of spinach grown on remediated soil. The research was conducted at the Screen House, Soil Laboratory, and Plant Protection Laboratory, Faculty of Agriculture, Universitas Jenderal Soedirman for four months. A randomized block design was used with treatment consisting of control and secondary metabolites application of T. harzianum T10 concentrations of 25, 50, 75, and 100%, repeated five times. The secondary metabolites were applied in the afternoon by pouring it on the soil in polybags and letting it stand for 10 days in tightly closed conditions. The variables observed were Fe content in the soil, plant height, number of leaves, shoot fresh weight, fresh root weight, and root length of spinach plants. The results showed that the secondary metabolites of T. harzianum T10 have the potential to remediate iron-congested soil. The content of Fe in the soil is 823 ppm. The appropriate concentration of T. harzianum T10 secondary metabolites as a remediator for Fe-contaminated soil is 50%, which can reduce the content of Fe (iron) by 46% compared to controls. The application of T. harzianum T10 secondary metabolites has not affected the growth and yield of spinach, which was grown on remediated soil, although there is a tendency to be better.