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All Journal PROSIDING SEMINAR NASIONAL Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences JURNAL KIMIA SAINS DAN APLIKASI The Journal of Experimental Life Sciences (JELS) Abdimas Sainteknol : Jurnal Sains dan Teknologi Bioeksperimen: Jurnal Penelitian Biologi Journal of Biology Education Unnes Science Education Journal Jurnal Penelitian Pendidikan IPA (JPPIPA) Biosaintifika: Journal of Biology & Biology Education Journal of Creativity Student Biopendix: Jurnal Biologi, Pendidikan dan Terapan Best Journal (Biology Education, Sains and Technology) Abdimasku : Jurnal Pengabdian Masyarakat Buletin Peternakan Tropis (Bulletin of Tropical Animal Science) Berkala Penelitian Hayati Unram Journal of Community Service (UJCS) Life Science Proceeding Seminar Nasional IPA Prosiding Seminar Nasional Biologi Unnes Science Education Journal Bioeksperimen Bioteach : Biology and Biology Education Journal Jurnal Ilmu Kesehatan Masyarakat (The Public Health Science Journal) Jurnal Abdimas Sainteknol : Jurnal Sains dan Teknologi Life Science
Dewi Mustikaningtyas
Fakultas MIPA, Universitas Negeri Semarang Semarang
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences Other

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Comparison of the Rate and Level of Biodegradation of Leaf Waste by Lignocellulolytic Molds from Universitas Negeri Semarang Campus Walandipa, Ghani; Mustikaningtyas, Dewi; Widiatningrum, Talitha; Dewi, Pramesti
Life Science Vol. 14 No. 2 (2025): November 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/unnesjlifesci.v14.i2.25828

Abstract

Biodegradation is a physical decomposition of the substrate caused by the activity of microorganisms by producing products that have benefits for humans. Lignocellulose degrading fungi are microorganisms that play a role in the process of decomposing organic waste. The biodegradation rate of each type of lignocellulose degrading mold is different. The purpose of this research is to comprehensively analyze and compare the rate and level of biodegradation of leaf waste in lignocellulolytic molds isolated from the campus environment of Universitas Negeri Semarang, as well as to identify and evaluate the type of lignocellulolytic mold that shows the highest efficiency in the rate and level of biodegradation of leaf waste. The method used in this research is quantitative method, by measuring the mass ratio and pH of leaf waste media before inoculation and after inoculation. The results of this study are the rate of leaf waste biodegradation based on the ratio of leaf waste mass by Trichoderma koningiopsis mold isolate of 0.015 grams/day, while by Trichoderma erinaceum mold isolate of 0.002 grams/day. Then the rate of leaf waste biodegradation based on the pH change value of leaf waste by Trichoderma koningiopsis mold isolate is higher with a pH change value of 0.3 when compared to the Trichoderma erinaceum mold isolate with a pH change value of 0.1. The superior performance of Trichoderma koningiopsis highlights its potential application in optimizing organic waste decomposition systems and provides a scientific basis for selecting lignocellulolytic fungi in sustainable waste management programs.
Development of a Research-Based Digital Teaching Supplement on Heavy Metal Pollution to Equip Junior High School Students with Digital Literacy Skills Sesami, Laut Padi Sih; Mustikaningtyas, Dewi
Unnes Science Education Journal Vol. 14 No. 3 (2025): December 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/usej.v14i3.32244

Abstract

Heavy metal pollution is an environmental problem that directly impacts human health and the sustainability of aquatic ecosystems. Research indicates that the issue of heavy metal pollution can be used as a contextual learning resource to foster students’ digital literacy skills and environmental awareness. Preliminary observations at SMP Negeri 1 Boja revealed that students’ digital literacy skills remain relatively low, indicating the need for innovative learning resources to enhance these competencies. This study aims to develop a research-based digital teaching supplement on heavy metal pollution to equip junior high school students with digital literacy skills. The research employed the ADDIE model, comprising the stages of analysis, design, development, implementation, and evaluation. The study involved eighth-grade students at SMP Negeri 1 Boja during the 2024/2025 academic year. Data analysis used descriptive statistics to assess validity and practicality, as well as percentage achievement analysis to evaluate students’ digital literacy. The results show that: (1) material expert validation scored 91.54% (very feasible) and media expert validation scored 93.07% (very feasible), (2) student responses indicated a practicality score of 93.06% (very practical), and (3) students’ digital literacy averaged 85.74% (very good) across the five aspects: accessing (90.00%), selecting (83.97%), understanding (90.15%), evaluating (80.44%), and producing (84.12%). These findings demonstrate that the developed research-based digital teaching supplement is highly feasible, highly practical, and effective in equipping students with strong digital literacy skills.
Isolation, Characterization, and Identification of Biosurfactant-Production Bacteria from Used Cooking Oil Waste Fisky Aditya Pratama; Pramesti Dewi; Ibnul Mubarok; Dewi Mustikaningtyas
Bioeksperimen: Jurnal Penelitian Biologi Vol. 11 No. 2 (2025): 2025
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v11i2.11115

Abstract

Waste cooking oil constitutes a significant environmental concern in Indonesia. It has the potential to be utilized as a substrate for biosurfactant-producing bacteria. Biosurfactants are amphiphilic compounds produced by microorganisms, containing both hydrophilic and hydrophobic components that enable them to dissolve in both water and fats, as well as to reduce surface tension. This study aims to isolate, characterize, and identify biosurfactant-producing bacteria from waste cooking oil. Isolation proccess was conducted using Mineral Salt Medium enriched with cooking oil as an inducer and Carboxy Methyl Cellulose as a carbon source and emulsifier. Two isolates were successfully obtained and purified. Their biosurfactant-producing ability was evaluated through drop collapse test and oil spreading test. One isolate demonstrated superior performance, showing positive activity in both tests, including a 0.7 cm oil spreading zone. Identification involved macroscopic observation, Gram staining, endospore staining, and a catalase test. The isolate exhibited characteristics of a Gram-positive, rod-shaped, spore-forming, catalase-positive bacterium with small, circular, milky-white colonies and a smooth surface. This identification is non-molecular and based on a phenotypic approach according to Bergey’s Manual for preliminary bacterial classification. Based on these features, the isolate is presumed to belong to the genus Bacillus, known for its robust biosurfactant production. The findings suggest that waste cooking oil can be a promising source of indigenous biosurfactant-producing bacteria, contributing to both microbial conservation and potential development of eco-friendly biotechnological products.
Co-Authors Achmadi, Taofan Ali Afiddah, Tawallani Andin Irsadi Andiniyati, Fitri Anggyi Trisnawan Putra Anisa Dewi Sri Puspitasari Annis Aulia Hastuti, Annis Aulia Apriansyah Wibowo Aprilia Indra Kartika, Aprilia Indra Ari Yuniastuti Arif Widiyatmoko, Arif Arvidhea Safira Gunawan Astrid Farmayati Atunnisa, Rifa' Aulia Nur Lita, Aulia Nur Azkiyah, Nimas Catur B., F. Putut Martin H. Bambang Priyono Budi Astuti Burhan, Ilham Damar Galih Darmawan, Melissa Salma Dewi, Novi Ratna Didik Ariwibowo Dinia Rizqi Dwijayanti Elissa, Intan Ayu Ely Rudyatmi, Ely Endah Peniati Fahmi Arifan Faj'ria, Nur Fajriyanti, Lisa Amelia Fatimah Azzahra Febriyanto, Hendra Fidia Fibriana Fisky Aditya Pratama Fitri Arum Sasi, Fitri Arum Fitriyah, Nanda Delva Grahadi, Rahmat Gunawan, Arvidhea Safira Hadikawuryan, Danang Subarkah Hafil Kusuma, Kavana Haris, Amnan Hasyim Abdurrasyid Heriyanti, Andhina Putri Hery Sutarto Hisan, Aisyah Khoirotun Hisan, Aisyah Khoirotun Ibnul Mubarok Ibnul Mubarok Ika Christina, Yuyun Indah Hartati Indriani, Betty Irawati Irawati Isnaeni Karunia Annisa Kaisar Akhmad Khan Kartika Widyaningrum Kholil, Putri Alifa Krispinus Kedati Pukan Kusuma, Kavana Hafil Listiaji, Prasetyo Maftuhah, Anis Maharani, Ria Ika Marlita Marlita, Marlita Martien Herna Susanti Milah, Nihayatul Moch Sasmito Djati Mohamad Endy Yulianto Muhammad Abdullah Na:am, Muh Fakhrihun Nafisah, Wirdatun Nashi Widodo Natalia, Lidya Ayu Nicky, Dhea Nikmatul Hidayah Noor Aini Habibah Nugrahaningsih Nugrahaningsih Nugrahaningsih WH, Nugrahaningsih Nugraini, Anisa Ratna Nur Rahayu Utami Nurul Fadhilah Nurussalma, Syafina Parmin - Pramesti Dewi Priyantini Widiyaningrum Purwadi, Cintiya Egi R Susanti R. Adi Susanti, R. Adi R. Susanti, R. Susanti Rahmawati, Maya Putri Rakainsa, Senda Kartika Ramadani, Mutiara Bintang Retno Sri Iswari Ria Ika Maharani Ria Ika Maharani Rizki Oktavia, Rizki Rizky Ajie Aprilianto Rizqi Dwijayanti, Dinia Rohmah, Ilmiana Nurur Romadhon, Vhaviriele Abel Salsabilla, Nur Hayati Afrilda Sasabila Mumtaz Sekar Jatiningrum Parnonansia Sekar Jatiningrum Parnonansia Sesami, Laut Padi Sih Sigit Saptono Siti Alimah Siti Harnina Bintari Siti Nur Chasanah, Siti Nur Sofiyani, Rakhma Dita Solichin Solichin Solikin, Ahmad Solikin, Ahmad Sri Sukaesih Sudarmin Sudarmin Sunarsih, Sarly Sutiyani, Iis Sutiyani, Iis Talitha Widiatningrum Trida Ridho Fariz Tuti Widianti Ufi Saraswati, Ufi Ulfa, Berlina Maika Ulung Anggraito , Yustinus Walandipa, Ghani Wara, Adisti Danis Weni Ratna Nurlita Hening, Weni Ratna Nurlita Wicaksono, Maulana Malik Widyananda, Muhammad Hermawan Winanti, Ruri Wiwi Isnaeni Wiyanto - Wiyanto Wiyanto Wulan Christijanti Wulansari Prasetyaningtyas, Wulansari Yustinus Ulung Anggraito Yuyun Ika Christina Zaenafree, Intan