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All Journal PROSIDING SEMINAR NASIONAL Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences JURNAL KIMIA SAINS DAN APLIKASI The Journal of Experimental Life Sciences (JELS) Abdimas Sainteknol : Jurnal Sains dan Teknologi Bioeksperimen: Jurnal Penelitian Biologi Journal of Biology Education Unnes Science Education Journal Jurnal Penelitian Pendidikan IPA (JPPIPA) Biosaintifika: Journal of Biology & Biology Education Journal of Creativity Student Best Journal (Biology Education, Sains and Technology) Abdimasku : Jurnal Pengabdian Masyarakat Buletin Peternakan Tropis (Bulletin of Tropical Animal Science) Berkala Penelitian Hayati Unram Journal of Community Service (UJCS) Life Science Proceeding Seminar Nasional IPA Prosiding Seminar Nasional Biologi Unnes Science Education Journal Bioeksperimen Bioteach : Biology and Biology Education Journal Jurnal Ilmu Kesehatan Masyarakat (The Public Health Science Journal) Jurnal Abdimas Sainteknol : Jurnal Sains dan Teknologi Life Science
Dewi Mustikaningtyas
Fakultas MIPA, Universitas Negeri Semarang Semarang
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences Other

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Isolation, Characterization, and Identification of Biosurfactant-Production Bacteria from Used Cooking Oil Waste Pratama, Fisky Aditya; Dewi, Pramesti; Mubarok, Ibnul; Mustikaningtyas, Dewi
Bioeksperimen: Jurnal Penelitian Biologi September 2025
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v11i2.11115

Abstract

Waste cooking oil constitutes a significant environmental concern in Indonesia. It has the potential to be utilized as a substrate for biosurfactant-producing bacteria. Biosurfactants are amphiphilic compounds produced by microorganisms, containing both hydrophilic and hydrophobic components that enable them to dissolve in both water and fats, as well as to reduce surface tension. This study aims to isolate, characterize, and identify biosurfactant-producing bacteria from waste cooking oil. Isolation proccess was conducted using Mineral Salt Medium enriched with cooking oil as an inducer and Carboxy Methyl Cellulose as a carbon source and emulsifier. Two isolates were successfully obtained and purified. Their biosurfactant-producing ability was evaluated through drop collapse test and oil spreading test. One isolate demonstrated superior performance, showing positive activity in both tests, including a 0.7 cm oil spreading zone. Identification involved macroscopic observation, Gram staining, endospore staining, and a catalase test. The isolate exhibited characteristics of a Gram-positive, rod-shaped, spore-forming, catalase-positive bacterium with small, circular, milky-white colonies and a smooth surface. This identification is non-molecular and based on a phenotypic approach according to Bergey’s Manual for preliminary bacterial classification. Based on these features, the isolate is presumed to belong to the genus Bacillus, known for its robust biosurfactant production. The findings suggest that waste cooking oil can be a promising source of indigenous biosurfactant-producing bacteria, contributing to both microbial conservation and potential development of eco-friendly biotechnological products.
Analysis of STEM Knowledge of Pre-Service Science and Mathematics Teacher Arif Widiyatmoko; Anggyi Trisnawan Putra; Budi Astuti; Senda Kartika Rakainsa; Hery Sutarto; Dewi Mustikaningtyas; Melissa Salma Darmawan
Jurnal Penelitian Pendidikan IPA Vol 10 No 3 (2024): March
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i3.5411

Abstract

This research is motivated by the importance of STEM knowledge for pre-service science and mathematics teachers in the 21st century. This research aims to analyze the STEM knowledge of pre-service science and mathematics teachers. The research method was carried out by distributing questionnaires with STEM knowledge indicators to the sample. The sample in this research was 86. They were 59 pre-service science teachers and 27 pre-service mathematics teachers, with different years of education, namely the 1st, 2nd, 3rd, and 4th year of Universitas Negeri Semarang. The results of this research show that the average understanding of pre-service science and mathematics teachers regarding to the STEM-based learning in the definition aspect is 4.11, objectives are 3.94, benefits are 3.94, aspects are 3.69, components are 3.71, characteristics is 3.75, and implementation is 3.86. This proves that pre-service science and mathematics teachers' understanding of the definition of STEM-based learning is very good, while pre-service science and mathematics teachers' understanding of the objectives, benefits, aspects, components, characteristics, and implementation of STEM-based learning is good.
Co-Authors Achmadi, Taofan Ali Afiddah, Tawallani Andin Irsadi Andiniyati, Fitri Anggyi Trisnawan Putra Anisa Dewi Sri Puspitasari Annis Aulia Hastuti, Annis Aulia Apriansyah Wibowo Aprilia Indra Kartika, Aprilia Indra Ari Yuniastuti Arif Widiyatmoko, Arif Arvidhea Safira Gunawan Astrid Farmayati Atunnisa, Rifa' Aulia Nur Lita, Aulia Nur Azkiyah, Nimas Catur B., F. Putut Martin H. Bambang Priyono Budi Astuti Burhan, Ilham Damar Galih Dewi, Novi Ratna Didik Ariwibowo Dinia Rizqi Dwijayanti Elissa, Intan Ayu Ely Rudyatmi, Ely Endah Peniati Fahmi Arifan Faj'ria, Nur Fajriyanti, Lisa Amelia Fatimah Azzahra Febriyanto, Hendra Fidia Fibriana Fitri Arum Sasi, Fitri Arum Fitriyah, Nanda Delva Grahadi, Rahmat Gunawan, Arvidhea Safira Hadikawuryan, Danang Subarkah Hafil Kusuma, Kavana Haris, Amnan Hasyim Abdurrasyid Heriyanti, Andhina Putri Hery Sutarto Hisan, Aisyah Khoirotun Hisan, Aisyah Khoirotun Ibnul Mubarok Ibnul Mubarok Ika Christina, Yuyun Indah Hartati Indriani, Betty Irawati Irawati Isnaeni Karunia Annisa Kaisar Akhmad Khan Kartika Widyaningrum Kholil, Putri Alifa Krispinus Kedati Pukan Kusuma, Kavana Hafil Listiaji, Prasetyo Maftuhah, Anis Maharani, Ria Ika Marlita Marlita, Marlita Martien Herna Susanti Melissa Salma Darmawan Milah, Nihayatul Moch Sasmito Djati Mohamad Endy Yulianto Muhammad Abdullah Na:am, Muh Fakhrihun Nafisah, Wirdatun Nashi Widodo Natalia, Lidya Ayu Nicky, Dhea Nikmatul Hidayah Noor Aini Habibah Nugrahaningsih Nugrahaningsih Nugrahaningsih WH, Nugrahaningsih Nugraini, Anisa Ratna Nur Rahayu Utami Nurul Fadhilah Nurussalma, Syafina Parmin - Pramesti Dewi Pratama, Fisky Aditya Priyantini Widiyaningrum Purwadi, Cintiya Egi R Susanti R. Adi Susanti, R. Adi R. Susanti, R. Susanti Rahmawati, Maya Putri Ramadani, Mutiara Bintang Retno Sri Iswari Ria Ika Maharani Ria Ika Maharani Rizki Oktavia, Rizki Rizky Ajie Aprilianto Rizqi Dwijayanti, Dinia Rohmah, Ilmiana Nurur Romadhon, Vhaviriele Abel Salsabilla, Nur Hayati Afrilda Sasabila Mumtaz Sekar Jatiningrum Parnonansia Sekar Jatiningrum Parnonansia Senda Kartika Rakainsa Sesami, Laut Padi Sih Sigit Saptono Siti Alimah Siti Harnina Bintari Siti Nur Chasanah, Siti Nur Sofiyani, Rakhma Dita Solichin Solichin Solikin, Ahmad Solikin, Ahmad Sri Sukaesih Sudarmin Sudarmin Sunarsih, Sarly Sutiyani, Iis Sutiyani, Iis Talitha Widiatningrum Trida Ridho Fariz Tuti Widianti Ufi Saraswati, Ufi Ulfa, Berlina Maika Ulung Anggraito , Yustinus Walandipa, Ghani Wara, Adisti Danis Weni Ratna Nurlita Hening, Weni Ratna Nurlita Wicaksono, Maulana Malik Widyananda, Muhammad Hermawan Winanti, Ruri Wiwi Isnaeni Wiyanto - Wiyanto Wiyanto Wulan Christijanti Wulansari Prasetyaningtyas, Wulansari Yustinus Ulung Anggraito Yuyun Ika Christina Zaenafree, Intan