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All Journal HAYATI Journal of Biosciences Jurnal Pengolahan Hasil Perikanan Indonesia ILMU KELAUTAN: Indonesian Journal of Marine Sciences Indonesian Journal of Cancer Chemoprevention BERITA BIOLOGI JURNAL BIOLOGI INDONESIA ANNALES BOGORIENSES Annales Bogorienses
Apon Zaenal Mustopa
Research Center for Biotechnology, Indonesian Institute of Sciences Jalan Raya Bogor km 46, Cibinong 16911, Bogor, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Immunology & microbiology Medicine & Pharmacology

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Journal : BERITA BIOLOGI

 1 
APLIKASI MARKA MOLEKULER PADA BUAH DAN BIJI KOPI ASAL KALIMANTAN TIMUR [Molecular marker application of cherry and green bean of East Kalimantan coffee] Fatimah, Fatimah; Urnemi, Urnemi; Mustopa, Apon Zaenal; Syahrumsyah, Hudaida
BERITA BIOLOGI Vol 13, No 1 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (133.562 KB) | DOI: 10.14203/beritabiologi.v13i1.649

Abstract

Two quantitative traits, cherry and green bean characters are the important phenotypic selection in coffee breeding practice. The important well-known character from coffee markets is cherry and bean size. In this study, 43 genotypes of coffee were collected from four districts in East Kalimantan i.e. Kutai Kertanegara, Kutai Timur, Berau dan Paser Utara. The objective of this study was to identify cherry and green bean character using quantitative trait locus (QTL) molecular marker, genetic variation from developed alleles, cluster analysis and association analysis of molecular marker, and phenotype observation. Based on polymorphic information content (PIC) of primers used in this study, the genetic variation was low. Based on cluster analysis, two major groups were identified. The first group corresponds to Arabika that consisted of 3 districts, Kutai Timur, Berau and Paser Utara. The second group correspond to Robusta mostly from Kutai Kertanegara.Significant association of primer markers M480 and M312 with QTL has suggested that they can be used as specific primers linked to size of cherry and green bean.Furthermore,they were potential marker assisted breeding in coffee breeding program.
CLONING, EXPRESSION, AND PARTIAL PURIFICATION OF PLANTARICIN W LOCUS PRODUCED BY Lactobacillus plantarum S34 [Kloning, Ekspresi, dan Purifikasi Parsial Lokus Plantarisin W Diproduksi oleh Lactobacillus plantarum S34] Umami, Rifqiyah Nur; Mustopa, Apon Zaenal; Sukmarini, Linda; Danuri, Hasim; Putri, Andini Setyanti; Wibowo, Krisna Dwi Aria
BERITA BIOLOGI Vol 16, No 1 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2760.052 KB) | DOI: 10.14203/beritabiologi.v16i1.2174

Abstract

Lactobacillus plantarum S34 dilaporkan mempunyai aktivitas antibakteri yang terkait dengan produksi bakteriosin. Bagian dari gen yang menyandikan salah satu lokus bakteriosin yang diproduksi oleh L. plantarum S34, disebut dengan plantarisin W (plnW), diamplifikasi dari plasmid dan dikloning menggunakan sistem vektor pGEM®-T Easy ke dalam Escherichia coli DH5?. Sekuens nukleotida plnW (± 405 pb) diidentifikasi sebagai protein integral membran. Lebih lanjut, plnW diekspresikan secara heterologus sebagai fusi protein dengan His(6)-tag tioredoksin menggunakan vektor ekspresi pET-32a(+) ke dalam E. coli BL21 (DE3) pLysS. Protein fusi rekombinan plnW terdapat dalam sitoplasma sel, tetapi selain fraksi terlarut terdapat juga fraksi tidak terlarut berupa badan inklusi. Purifikasi parsial dilakukan menggunakan kromatografi afinitas ligan Co2+ untuk fraksi terlarut dan metode elektroelusi gel poliakrilamid untuk fraksi tidak terlarut. Massa molekul berukuran kurang lebih 33 kDa terdeteksi berdasarkan pemisahan SDS-PAGE dan dikonfirmasi dengan Western blot sebagai protein fusi rekombinan plnW. Protein yang sudah terpurifikasi bermanfaat untuk mengetahui kaitan antara struktur dan fungsi bakteriosin.
Co-Authors Ai Hertati, Ai Akhmad Solikhin Akmaliyah, Rizna Alfisyahrin, Wida Nurul Amani, Febriyanti Nur Amelia, Suci Amelia, Suci Andi Asmawati Azis Anika Prastyowati, Anika Anja Meryandini Anna Ida Sunaryo Purwiyanto Arifah, Rosyida Khusniatul Ario Betha Juanssilfero Chairunnisa, Sheila Chihombori, Tatenda Calvin DWI SUSILANINGSIH Ekawati, Nurlaili Eva Johannes Farida, Hilda Farida, Hilda Fatimah fatimah Fatimah Fidien, Khadijah Alliya Fidien Firdaus, Moh Egy Rahman GINA KARTINA Harsi D. Kusumaningrum Hartati Chairunnisa Hartoni Hasim - Hasim Hasim Huda Shalahudin Darusman Hudaida Syahrumsyah, Hudaida I wayan Teguh Wibawan Ikramullah, Muh. Chaeril Iman Rusmana Irawan, Herman Irawan, Shasmita Isworo, Rhestu Isworo, Rhestu Jendri Mamangkey Joko Pamungkas Joko Wahyono, Daniel Kartika Sari Dewi Kusdianawati Kusmajadi Suradi Kustiariyah Tarman Kusumawati, Arizah LINDA SUKMARINI Lita Triratna Magdalena Litaay Maggy Thenawidjaja Suhartono Mala Nurilmala Manguntungi, Baso Maulidiani, Maulidiani Meilina, Lita Meiyerani, Jeni Melki Mustafawi, Wike Zahra Mustika Tuwo, Mustika Mutiara, Ilma Nina Herlina Novianti, Ela Nur Amani, Febriyanti Nur Umami, Rifqiyah Nurfatwa, Maritsa Pandayu, Iga Firmansyah Popi Hadi Wisnuwardhani, Popi Hadi Puspo Edi Giriwono Putri, Andini Setyanti Putri, Prabawati Hyunita Putri, Prabawati Hyunita Ramadhian, M. Zalfa RATIH ASMANA NINGRUM Ratna, Lita Tri Refli, Redoyan Rifqiyah Nur Umami, Rifqiyah Nur Rika Indri Astuti Saputri, Dinar Suksmayu Saputri Sari, Isti Kartika Sofyana, Neng Tanty Sri Swasthikawati, Sri Suharli, Lili Suharsono Suharsono Surachma, Rahma Sari Tri Wibawa Trinugroho, Joko P Urnemi Urnemi, Urnemi Vanggi, Leggina Rezzy Vanggy, Leggina Rezzy Wahyuni, Ike Wahyuni, Ike Wardhani, Widdya Kusuma Wardhani, Widdya Kusuma Wendry Setiyadi Putranto Wibowo, Krisna Dwi Aria Wijaya, Hans Wike Ayu Eka Putri Yudi Purnomo