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All Journal Jurnal Sain Veteriner Jurnal Veteriner Journal of Food and Life Sciences Journal of Innovation and Applied Technology Jurnal Ternak Tropika Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Medik Veteriner Vitek : Bidang Kedokteran Hewan Veterinary Biomedical and Clinical Journal Jurnal Nutrisi Ternak Tropis (JNT) Jurnal Pengabdian Masyarakat Asia Rambideun : Jurnal Pengabdian Kepada Masyarakat Prosiding Seminar Nasional Unimus
Yudit Oktanella, Yudit
Departement Of Veterinary Reproduction, Veterinary Medicine Faculty, Brawijaya University, Puncak Dieng, Dau, Malang, East Java 65151, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Economics, Econometrics & Finance Education Engineering Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary Other

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Journal : Jurnal Veteriner

 1 
Polimorfisme Gen Oviductal Glycoprotein-1 pada Oviduk Kambing Peranakan Etawa Penderita Kista Ovarium (OVIDUCTAL GLYCOPROTEIN-1 GENE POLYMORPHISM IN THE OVIDUCT OF ETTAWA CROSSBREED GOATS SUFFERING FROM OVARIAN CYST) Herawati Herawati; Febry Sysdityawan Ramadhan; Dyah Ayu Oktavianie; Yudit Oktanella
Jurnal Veteriner Vol 21 No 2 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (169.512 KB)

Abstract

Ovarian cysts are a disorder characterized by the growth of smooth muscle cells in the ovaries forming like fluid-filled balloons. Oviductal Glycoprotein (OVGP1) is secreted in the oviduct and plays a role in early embryonic development. The incidence of ovarian cysts is thought to affect the level of expression of the OVGP1 gene. The purpose of this study was to determine the potent role of OVGP1 gene as biomarker for detection of ovarian cyst on goats by Polymerase Chain Reaction (PCR) method. The samples used were DNA isolated from normal female PE goat oviduct and goat with follicular ovarian cyst obtained from RPH. The OVGP1 gene identification was performed by PCR method using 5'GACTGCAACCCACACAAGGA-3 forward primer (OVN1 _F) and reverse primer (OVN1_R) 5'GAGAGCAGCACCCACTTTTC-3 ‘. The sequencing of PCR results is done by Sanger method. The gene sequence analysis used the Bioedit and NCBI BLAST programs by comparing the PCR sequence on the follicular ovarian cyst and the normal ovary with the OVGP1 gene of the gene bank NM_001285584.1. The results showed that two normal samples and two samples of follicular cysts had similarities above 90% with OVGP1 gene bank NM_001285584.1. Samples with follicular ovarian cysts indicate a change in nucleotide base sequence due to deletions, insertions, transitions and transversions that affect the amino acid sequence. The arrangement of amino acids on samples with follicular ovarian cysts found an increase in the number of proline (P), threonine (T) and serine (S). In conclusion there is a difference in OVGP1 gene sequences in sample follicular ovarian cysts compared with sample normal ovaries . So the OVGP1 gene potentially can be used as a marker of follicular ovarian cysts.
Penambahan Osteopontin dalam Pengencer Semen Beku Sapi Perah Friesian Holstein Meningkatkan Ekspresi B-Cell Cll/Lymphoma-2 Spermatozoa Postthawing (ADDITIONAL OSTEOPONTIN INTO FROZEN FRIESIAN-HOLSTEIN SEMEN DILUTER INCREASES THE EXPRESSION OF B-CELL CLL/L Abdul Samik; Yudit Oktanella; Tatik Hernawati; Ngakan Made Rai Widjaja; Intan Purwa Dewanti
Jurnal Veteriner Vol 15 No 4 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Post thawed frozen semen viability is one of the most important keys in artificial inseminationdepended on two major cell death mechanism, apoptosis and necrosis. It has been known that good fertilitydiary bull’s seminal plasma contains high concentration of osteopontin (OPN). Osteopontin also known ascell survival protein via inhibition to apoptotic cell death, and B-cell CLL/Lymphoma-2 (Bcl-2)expressionmostly related to the ability of cell survival. The aim of this study was to investigate the influence ofadditional OPN into frozen semen diluter on post thawed sperm Bcl-2 expression. Fresh semen collectedfrom ±4 year Friesian Holstein bull. Treatment group divided into four groups i.e.: control group (P0:without OPN supplementation), (P1: fresh semen with OPN supplementation 5 ?g/5.107 spermatozoa), (P2:fresh semen with OPN supplementation 10 ?g/5.107 spermatozoa), (P3: fresh semen with OPNsupplementation 20 ?g/5.107spermatozoa). Bcl-2 sperm expression was determined usingimunocytochemistry. The result of this study showed that there was no significant difference betweengroup P0 and P1 (p>0,05), but both group P2 and group P3 showed a significant difference with P0 (p<0,05).Nevertheless, there was no significant difference between group P2to group P3 on post thawed FriesianHolstein sperm Bcl-2 expression (p>0,05). The conclusion of this study is osteopontin supplement in frozensemen diluter is capable to increase post thawed Friesian Holstein sperm Bcl-2 expression.
Tyrosine Kinase Gene Polymorphisms in Limousin (Bos taurus) Bull Correlation with Fresh Semen Qualities Ageng Ilham Ramadhani; Yudit Oktanella; Wawid Purwatiningsih; Andreas Bandang Hardian; Tatik Hernawati
Jurnal Veteriner Vol 23 No 4 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2022.23.4.480

Abstract

Tyrosine kinase (TEK) is the subgroup of protein kinases class. Tyrosine kinase (TEK) is a protein in spermatozoa plasm that functions as a mediator during spermatozoa penetration to pellucide zone 3 (PZ3) of the ovum. The purpose of this study was to analyse the variation of receptor tyrosine kinase genes related to the fresh semen qualities of limousin bulls, using Polymerase Chain Reaction Restriction Fragment Length Polymorphism (PCR-RFLP) method with HindIII restriction enzyme in A/AGCTT restriction site. This study used 20 blood samples of limousin bull from BBIB Singosari Malang. DNA amplification carried out by PCR method using forward primer (TEK_F) 5'-TAGATTGTCGCTTGCCTGGG-3’ and reverse primer (TEK_R) 5'-CCTGTGCCGACAGGTTTACT-3’. The data analysis were obtained by counting the total of RFLP DNA fragments as the genetic variation. The correlation between the total of RFLP DNA fragments and the fresh semen qualities were analysed by Spearman correlation test with RStudio software. The polymorphic DNA fragments were on 136 bp, 88 bp, 72 bp, and 12 bp range. Monomorphic DNA fragment was on 39 bp range. Correlation coefficient between the RFLP DNA fragments total with semen pH was -0,158 (negative correlation) with the p-value 0,912; semen volume was -0.105 (negative correlation) with the p-value 0,659, sperm motility was -0,050 (positive correlation) with the p-value 0,831; and sperm concentration was 0,044 (positive correlation) with the p-value 0,852. The RFLP DNA fragment total with some fresh semen evaluation parameters had very low correlation with no significancy because of the p-value >0,05.
Co-Authors Abdul Hakim Abdul Samik Aditya, Siska Adrenalin, Sruti Listra Ageng Ilham Ramadhani Agung Wicaksono Agus Budiarto Agustina, Galuh Candra Agustina, Galuh Chandra Ahmad Lukman Hadiprawoto Amri, Indah Amalia Arvel Risky Widyana Aryani, Dhita Evi Assifa Mardiyah, Fraya Cahyani, Ayunda Ageng Dameanti, Fidi Nur Aini Eka Puji Desi Wulansari Desi Wulansari Dian Vidiastuti, Dian Dyah Ayu Oktavianie Edwin Widodo Elfahra Casanza Amalda Farah Alhamidah Febry Sysdityawan Ramadhan Firmawati, Aulia Fraya Assifa Mardiyah Galuh Chandra Agustina Gatot Ciptadi Hardian, Andreas Bandang Herawati . Herawati Herawati Indah Amalia Amri Intan Purwa Dewanti Kamulyan, Ujiati Kusumarini, Shelly Mariyam, Dewi Muhammad Arwani Muhammad Khuzain Ngakan Made Rai Widjaja Prasetyo, Gabriela Jeanne Giacynta Purwatiningsih, Wawid Qosimah, Dahliatul Raden Satrio Mukti Renanda Nur Al Jabbar Rizka Gitta Almaida Sruti Listra Adrenalin Tatik Hernawati Ujiati Kamulyan Viski Fitri Hendrawan Viski Fitri Hendrawan Wawid Purwatiningsih Widi Nugroho Yesica, Reza Zita Viera Pradnya R.