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A. Shofy Mubarak
Departemen Kelautan, Fakultas Perikanan Dan Kelautan, Universitas Airlangga
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Kemampuan Reproduksi Daphnia magna Jantan Hasil Induksi Logam Berat (Cd, Pb) Dan Pestisida Diazinon [Reproductive Ability Of Male Daphnia magna Induction Results Of Heavy Metal (Cd, Pb) And Pesticide Diazinon] A. Shofy Mubarak; Desi Nawang Purnamasari; Laksmi Sulmartiwi; Sudarno Sudarno
Jurnal Ilmiah Perikanan dan Kelautan Vol. 2 No. 2 (2010): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v2i2.11643

Abstract

Abstract Daphnia magna is one of the natural food which is used widely in fish hatchery. Generally, Daphnia magna is used widely in larval rearing of fish that are traded in the form of resting eggs called ephipia. Ephipia production can be performed with Daphnia magna culture in conditions that are not optimal, one of which is pollution. If the conditions are not optimal then the parthenogenesis Daphnia magna would produced a male individual. The presence of Daphnia magna males will initiate sexual reproduction, where Daphnia magna males will perform on the female and copulation produce ephipia. Ephipia production is influenced by the efficiency of matting, reproduction ephipia will improve if a high mating eficiency of Daphnia magna. Mating eficiency describes the number of females that successflly fertilized by the male.The purpose of this study is to determine the reproductive capacity of Daphnia magna male's induction of heavy metals (Cd, Pb) and the Pesticide Diazinon on mating efficiency and quality ephipia produced. The research method used is an experimental method by using completely randomized design with 4 treatments and 5 replications so that was followed by Duncan Multiple Range Test. This study uses the ratio of male to female sex 1:30 for each treatment. The treatments in this study consist of control males mated with females, male induced lead (Pb) were mated with female, males induced cadmium (Cd) were mated with females, the male mated with the induction of pesticide Diazinon female. Results showed that treatments using natural male produced an average value of mates which were the highest efficiency and quality ephipia optimal, with precentage of 88,66 and 88,71. So that in order to produce ephipia in large quantities and quality needed natural male culture can be done in the unpolluted waters.
Penambahan Ekstender Madu Dalam Proses Penyimpanan Sperma Beku Terhadap Motilitas Dan Viabilitas Spermatozoa Ikan Komet (Carassius auratus auratus) [Additions Extender Honey In Frozen Sperm Storage Process Against Sperm Motility And Viability Comet Fish (Carassius auratus auratus) ] Boedi Setya Rahardja; A. Shofy Mubarak; Permana Sulistyo Rini
Jurnal Ilmiah Perikanan dan Kelautan Vol. 2 No. 2 (2010): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v2i2.11649

Abstract

Abstract The development of fish culture is strongly influenced by hatchery technology, especially in the stock of fish seed. Often, the problem arises in the stock of seeds, due to the maturation of gametes, broadstock fish sometimes do not happen simultaneously, one of alternative solution to the problem through the application of reproductive biotechnology, is preservation of sperm (Dirjennak, 2007). Preservation sperm is to optimize the male broadstock (Dirjennak, 2007). This storage process requires diluent and cryoprotectant material that could sustain sperm motility (Zaenab, 2007). Diluent material is used to reduce the activity of spermatozoa that inhibit energy usage and prolong the life of sperm (Rustidja, 2000). The use of honey as a diluent material is expected to support the vitality of spermatozoa. Material of honey there are various combinations of materials a simple sugar and salt ions. With the combination of basic ingredients and simple sugar salt ions spermatozoa can produce an energy source so it can defend itself and can fertilize an egg cell after Cryoperservation process. The purpose of this research is to know the effect of adding extender honey in enhancing sperm motility and viability of fish comet after the freezing process and to determine the best dose of honey in the process of freezing sperm comet fish (Carassius auratus auratus). This research was conducted at the Laboratory Center for Artificial Insemination Singosari. The design use Completely Randomized Design (CRD) followed by Duncan's multiple range test. Test materials in this research is sperm comet fish are packed in mini straw and stored in liquid nitrogen container with 9 treatments and 3 replications. Media diluent used was physiological NaCl is added to honey, glucose, fructose, and Tris Aminomethane. Honey dosages of the experiments were 0.3% (A); 0.4% (B), 0.5% (C), 0.6% (D) and 0.7% (E) and 0.05% glucose (KG); fructose 0.05% (KF), Tris Aminomethane (KT) and without the addition of physiological NaCl (KN). The main parameters of observed percentage of live sperm and duration of motion. The supporting parameters measured were fresh sperm concentration, percentage of live sperm fresh, long fresh sperm, pH, volume and color of sperm. The results of the research shows that the addition of honey with different dosage at diluents material physiological NaCl gived significant effect (P <0.05) against the percentage of sperm motion and long life. Average percentage of long life and highest sperm obtained by treatment of the addition of honey 0.6% (D), ie 63.89% and 101.33 seconds. Average lowest percentage of life gained by treatment of honey 0.3% (C) that were 39.67 second while the lowest was in motion treatment control without the addition of (KN) that were 28.33 seconds.
Korelasi Antara Konsentrasi Oksigen Terlarut pada Kepadatan yang Berbeda dengan Skoring Warna Daphnia spp. [Correlation Between Dissolved Oxygen Concentration in Different Densities with Color Scoring Of Daphnia spp.] A. Shofy Mubarak; Diah Ayu Satyari U; Rahayu Kusdarwati
Jurnal Ilmiah Perikanan dan Kelautan Vol. 2 No. 1 (2010): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v2i1.11665

Abstract

Abstract Dissolved oxygen is the parameters key of water quality. Low level of dissolved oxygen can affect the function and slow growth, can even cause death to fish in hypoxia condition. The aims of this experiment were to determine the effect of population density of Daphnia spp. of dissolved oxygen concentration and the correlation between dissolved oxygen concentration with color scoring of Daphnia spp. This method was experimental with Completely Randomized Design as experiment design with 6 treatments and 4 replications. The treatment that was given were the differences of Daphnia spp. density that consisted of treatment A (50 individu/200 ml), treatment B (250 individu/200 ml), treatment C (450 individu/200 ml), treatment D (650 individu/200 ml), treatment E (850 individu/200 ml), and treatment F (1050 individu/200 ml). The results showed that the difference of Daphnia spp. density showed highly significant effect of dissolved oxygen concentration of treatment medium (p<0,01). In addition, this experiment also showed that the dissolved oxygen concentration in treatment medium showed negative correlation with color score of Daphnia spp.
Pengaruh Pemaparan Logam Berat Pb (Timbal) Terhadap Perubahan Warna dan Peningkatan Persentase Anakan Jantan Daphnia spp. [Effect Of Heavy Metal Pb (Lead) Expossure To The Color Change and Increase The Percentage Of Male Offsprings Of Daphnia spp. ] A. Shofy Mubarak,; Akhsan Panna; Yeni Dhamayanti
Jurnal Ilmiah Perikanan dan Kelautan Vol. 1 No. 2 (2009): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v1i2.11686

Abstract

AbstractPb (lead) was a metal that does'nt regulated by water organisms so that the metal was constantly acumulated in organisms tissue. Currently, the pollution Pb in the waters of Indonesia have exceeded the maximum threshold of pollution defined by the government.Daphnia spp. is the organism that have been developed as a bioassay in some developed countries like U.S. and Japan because it has a rapid life cycle that is about three weeks, are sensitive to the chemical in the waters ecology and has an important role in the ecology of freshwater as the first of trophic level in the waters ecology. Target of this research is to obtain information about the concentration of heavy metals Pb that correlated with of Daphnia spp. color changes and increase of male offsprings of Daphnia spp.. This research was th thconducted on november 24 , 2008 until December 8 , 2008 in the Laboratory Education of fisheries, Faculty of Fisheries and Marine, Airlangga University. This research use method of experimental design with Completely Randomized Design (RAL)  with five treatments and four rerplicates. Treatment A (control), exposure of Pb in concentration 0 mg/L. Treatment B, Pb concentration 0.90 mg/L, Treatment C, Pb concentration 1.8 mg/L, Treatment D, Pb concentration 2.7 mg/L, and Treatment E with Pb concentration 3.63 mg/L. Collected data were analyzed using Analysis of Variance (ANOVA) with error rate or á = 0,05, if there are a different, hence continued with Duncan's Multiple Range Test. Collected data were analyzed again using linear regression graph to estimate the enhanced in every treatment. The results of research indicates that the heavy metal Pb exposure with different concentrations to the adult female Daphnia spp. did not showing significant different of the daphnia spp. color change. This is because the condition of Hypoxia (oxygen deficiency) more dominant than the increase of methyl farnesoate to influence increase of Daphnia spp.'s haemoglobin synthesa. Besides, research also indicates that the heavy metals Pb exposure with different concentrations to the adult sex Daphnia spp. provide a very significant percentage of male Daphnia spp. offsprings. This happens due to the increase of methyl farnesoate in Daphnia spp. inhibit the formation of female sex of Daphnia spp. offsprings by adult Daphnia spp. that expossured by Pb. Result of processing data using linear regression is a formula y = 18.76x + 3.104. this formula can be used to estimate concentration of Pb in water based on percentage of male offsprings of Daphnia spp. Water quality during research showed the waters pH range between 8.2 - 8.5,  dissolved oxygen (DO) oranged between 8.0 - 8.5 mg/L, waters temperature was 26 C and ammonia level was 0.03 mg/L. This Conditions of water quality was the optimal conditions to support Daphnia spp. life. 
Pengaruh Penambahan Madu Dalam Pakan Induk Jantan Lobster Air Tawar Red Claw (Cherax quadricarinatus) Terhadap Rasio Jenis Kelamin Larva [The Effect Of Suplementation Of Honey In Feed Of Male Mains Fresh Water Cray Fish Red Claw (Cherax quadricarinatus) To Larva Sex Ratio ] A.Taufiq Mukti; A.Shofy Mubarak; Adde Ermawan
Jurnal Ilmiah Perikanan dan Kelautan Vol. 1 No. 1 (2009): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v1i1.11696

Abstract

Abstract The aim of this research was to know the effect of honey supplementation in feed of male main freshwater cray fish red claw to larva sex ratio and the level of survival of freshwater cray fish and to get the best doses of honey to give highest male sex ratio. This research was done at Laboratory of Fishery Education, Fishery and Marines Faculty, Airlangga University in October 2007 until March 2008. Methods of this research was experimental and used completely randomized designed with 7 treatments. Giving of honey with differences doses were A ( 0 ml/kg) control, B ( 50 ml /kg), C ( 100 ml /kg), D ( 150 ml /kg), E ( 200 ml /kg), F ( 250 ml/kg) and G ( 300 ml/kg) which each treatment got three times replicated. Data processed by Analysis of Variance (ANOVA). The result of this research showed that supplementation of honey in feed male main freshwater cray fish red claw not significantly different to larva sex ratio and survival rate of freshwater cray fish. Otherwise, result of the supplementation of honey in feed of male main freshwater cray fish showed the increasingly male sex ratio of freshwater cray fish red claw larvae.
Pengaruh Konsentrasi Kadmium Terhadap Perubahan warna Dan Persentase Jenis Kelamin Jantan Anakan Daphnia magna [Influences Concentration Of Cadmium To Colour Change And Male Sex Percentage Neonates Daphnia magna] Rahayu Kusdarwati; Alfi Hermawati W; Setyawati Sigit; A. Shofy Mubarak
Jurnal Ilmiah Perikanan dan Kelautan Vol. 1 No. 1 (2009): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v1i1.11697

Abstract

Abstract Cadmium is one of dangerous heavy metal and it can cause water pollution. As an alternative early warning toward heavy metal in water, Daphnia magna can be used as biology indicator water pollution that caused by heavy metal. Daphnia magna is organism that ussualy used for bioassay in many progressing countries, because that organism have important role in freshwater ecology, short life cycle about 3 weeks and sensitive toward chemical environment. Grade of toxicity heavy metal can measured with lethal and sub lethal parameter. In Daphnia magna, sub lethal parameter that can be observe are colour changes and male sex percentage neonates. The purpose of this research was to know the colour changes and male sex percentage neonates Daphnia magna in different concentration of cadmium. Target of this research is to gets information about level colour changes and male sex percentage neonates Daphnia magna in different concentration of cadmium. The main parameter was colour changes and male sex percentage neonates and the secondary parameters were water quality (temperature, pH, dissolved oxygen and NH3) The result of research of giving different cadmium concentration to the adult female Daphnia magna not showing significant influence, nevertheless it is showing significant influence toward male sex percentage neonates Daphnia magna. The treatment with cadmium concentration 0,0004 mg/l produce 100% male neonates Daphnia magna. Water quality during research were in optimal conditions to support Daphnia magna life, those are temperature 26°C, pH range from 8,0 – 8,2 and dissolve oxygen (DO) range from 8,5 – 9,0 mg/l and ammonia 0 – 0,03 mg/l.
Pemberian Dolomit Pada Kultur Daphnia spp. Sistem Daily Feeding Pada Populasi Daphnia spp. dan Kestabilan Kualitas Air [Dolomite Added At Culture Of Daphnia spp. Daily Feeding System At Daphnia spp. Population and Water Quality Stability]. A. Shofy Mubarak; Laksmi Sulmartiwi; Diah Trie Ridyaning Tias
Jurnal Ilmiah Perikanan dan Kelautan Vol. 1 No. 1 (2009): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v1i1.11700

Abstract

Abstract Daphnia spp. was potential source of live feed to be developed for larvae of freshwater fish. One of maximize culture technology was daily feeding system with rice bran solution. This system able to increase Daphnia spp. population. The problem of daily feeding culture technology was water quality stability, such as high pH fluctuation and high ammonia concentration. Water pH stability and decrease of ammonia concentration could be increased by dolomite added which function as stabilize pH buffer. The aim of this research found out the dolomite dose and culture period that able to increase population and stabilize of water quality. Experimental design was Completely Random Design with 4 replicates those were A control (0 mg/l), B (50 mg/l), C (100 mg/l), D (150 mg/l), E (200 mg/l) and F (250 mg/l). Parameter that measured were Daphnia spp. population and water quality, such as temperature, pH, ammonia, dissolve oxygen and alkalinity. The data were examined using ANAVA(Analysis of Variance) to know treatment effect and be continued by Duncan Multiple Range with trust of degree 0,05. for water stability using descriptif methode. Result of analysis showed that high population for all treatment were held on 6th, 7th and 8th day with the highest Daphnia spp. population was held on 8th day by F treatment (250 mg/l) were 9900 Daphnia spp./l. Water quality data showed different between each treatment.
Primary and Secondary Sexual Characteristics of Kuhli Loach (Pangio kuhlii) Budi, Darmawan Setia; Restanti, Maya; Suciyono, Suciyono; Müller, Tamás; Kenconojati, Hapsari; Mubarak, Ahmad Shofy
Jurnal Medik Veteriner Vol. 7 No. 2 (2024): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss2.2024.264-275

Abstract

Kuhli loach (Pangio kuhlii) a popular ornamental freshwater fish species with economic potential, originating from Sumatra, Kalimantan, Java, and Malaysia. The kuhli loach's appeal lies in its vibrant colors and distinctive eel-like body. This study aims to explores the sexual primary and secondary characteristics of the kuhli loach. Conducted at Universitas Airlangga in Banyuwangi, Indonesia, the study involved a thorough analysis of 50 kuhli loaches, evenly split between males and females. Morphological observations revealed significant differences (p < 0.05), such as the elongated body and expanded abdominal region in females, suggesting adaptations for reproduction. Internal examinations, including peritoneal dissections and histological analysis, unveiled distinct stages of spermatogenesis in males and ovarian development in females, providing insights into the reproductive cycle. Meristic and morphometric analyses identified noteworthy differences in pectoral fin rays, suggesting potential roles in reproductive behavior, while other traits exhibited similarity between genders. This study contributes to the understanding of kuhli loach ecology and reproductive biology, highlighting the importance of addressing sustainability concerns arising from the continuous exploitation of wild populations. This study serves as a foundational step towards advancing reproductive improvement in fish and supports the conservation of this unique ornamental fish species, with implications for aquaculture practices and economic considerations.
The Effect of Different Steaming Temperatures on Albumin Levels of Scad Fish (Decapterus ruselli) Fitrawan, M. Dzaky; Shofy Mubarak, Ahmad; yuli pujiastuti, dwi
Journal of Marine and Coastal Science Vol. 12 No. 1 (2023): FEBRUARY
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmcs.v12i1.42660

Abstract

Albumin is a plasma protein that consists of a single polypeptide chain and is used to accelerate the recovery of split/damaged cell tissue. Albumin is produced from human blood in the form of human serum albumin (HSA) which is fulfilled through imports at a relatively expensive price. The alternative albumin is needed to get the cheaper albumin but has the same clinical aspects, such as albumin from the scad fish (Decapterus ruselli). The purpose of this study is to determine the effect of different steaming temperatures on the albumin levels of scad fish (Decapterus ruselli) and get the optimal steaming temperature used to obtain albumin levels from scad fish (Decapterus ruselli). This research was conducted by the Completely Randomized Design (CRD) method which consists of 4 different steaming temperature treatments with 5 replications. The parameters observed were albumin content, protein content, and yield. The parameters were tested using One Way Analysis of Variance (ANOVA) followed by the Duncan Multiple Range Test. The results of this study showed that the treatment of different steaming temperatures had a significant effect (p<0.05) on the albumin levels and percentage of protein content. The optimum steaming temperature to obtain the highest albumin content from scad fish species Decapterus ruselli is 35℃ with an albumin content of 0.1394 g/L. Keywords: Scad fish, steaming, albumin, protein, yield
Growth and Development of Stadia Artemia salina in Culture with Different Concentration of Bran Suspension Cahyani Prasetyawati, Fatimah; Shofy Mubarak, Ahmad; Setya Rahardja, Boedi
Journal of Marine and Coastal Science Vol. 13 No. 2 (2024): JUNE
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmcs.v13i2.52455

Abstract

The annual need for Artemia in Indonesia reaches 40-60 tons, but up to now 100% of this need is still imported. Various efforts have been made to produce Artemia cysts, but the main obstacle in cultivating Artemia to produce cysts is the availability of natural food, namely phytoplankton which can survive in high salinity. The use of organic materials such as bran can be a solution for feeding and can increase the density of Artemia, but excessive use of bran suspension in Artemia cultivation ponds can trigger a decrease in the quality of the water for the growth of Artemia. This research was conducted to determine the optimal concentration of bran feed for the growth and development of Artemia salina without compromising the water quality of the cultivation. The Artemia feed used in this study was a bran suspension enriched with lemuru fish oil (12 ml/L bran suspension). The bran feed concentration treatments in this study were 5 treatments sequentially, namely P1 to P5 with feed concentrations of 11, 18, 26.34, and 41 mg/L. Artemia salina cultivation in this study was carried out at an initial density of 50 ind/L with a salinity of 30 pptl. The research results showed that cultivating Artemia salina using bran suspension feed with different concentrations had an effect on the growth and development of Artemia salina. The best growth and development of Artemia results from cultivation using bran suspension feed with a concentration of 41 mg/L.
Co-Authors A. Taufiq Mukti A.Taufiq Mukti Adde Ermawan Adriana Monica Sahidu Akhmad Taufiq Mukti Akhsan Panna Alfi Hermawati W Amilaningrum, Diadara Amin, Muhamad Nur Ghoyatul Annisa Nur Rohmah Purnamasari Apriana Vinasyiam Arum, Kartika Dwi Sekar Boedi Setya Rahardja Browijoyo Santanamurti Cahyani Prasetyawati, Fatimah Carattri, Money DARMAWAN SETIA BUDI DEDI JUSADI Desi Nawang Purnamasari Destya Twinandia Diah Ayu Satyari U Diah Trie Ridyaning Tias Diana Meritasari Djamil, Hasim Dwi Yuli Pujiastuti Eka Ainurrohmah Eka Saputra Eka Saputra Endang Dewi Masithah Endang Tri Wahyurini Faricha Risma Nurani Fitrawan, M. Dzaky Gunanti Mahasri Heru Pramono Husein, Mohamad Akmal Alwi Intan Cahyarini Isroni, Wahyu Juanda Juanda Julie Ekasari Kamiliya Zahrah Taher Kenconojati, Hapsari Kismiyati , Laksmi Sulmartiwi Layuk, Tresia Sanda Likanimasayu Prabandari M. Zairin Junior Manik, Melani Indah Sari Maulida Rosa Umainana Maulidia, Cici Mayangsari, Cholivia Melani Indah Sari Manik MUHAMMAD AGUS SUPRAYUDI Muhammad Daffa Al Rasyid Nurhayati Müller, Tamás Mulyono Mulyono Munjayana, Munjayana N. Juni Triastuti Ninin C Nurmalia Dewi, Nina Patmawati Patmawati, Patmawati Permana Sulistyo Rini Pursetyo, Kustiawan tri Puspitasari, Febriyanti Putri Alia Paramitha Putri, Monica Angelina R. Rahardyan Prasetyo Rahayu Kusdarwati Rahmadani Rahmadani Ramadhan, Novelix Arditan Ratna Diyah Palupi Reni Oktaviani Restanti, Maya Rochmatika, Endah Rohmatin, Aulia Rokhim, Akhmad Nur Rr. Juni Triastuti Sapto Andriyono Saragih, Friska Setiawani Sari, Devi Elvina Septuresty Hartri Eka Setyawati Sigit Sihombing, Agusmanto Steffani Lutfi Yuni firdausi Suciyono, Suciyono Sudarno, Sudarno Utari, Heny Budi Veryl Hasan Widigdo, Syafrianur Win Darmanto Wiyoto Wiyoto, Wiyoto Woro H. Satyantini Woro Hastuti Satyantini Woro Hastuti Setyantini Wulan, Novrida Yeni Dhamayanti Yuli Pujiastuti, Dwi Yusuf Taufik Hidayat Zurica Melati Fitri