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The Characterization of Collagen Isolated from Red Snapper Fish Skin (Lutjanus sp.) by Hydroextraction Method with Different Concentration of Acid Solution R. Rahardyan Prasetyo; Ahmad Shofy Mubarak; Eka Saputra; Juni Triastuti
Journal of Aquaculture and Fish Health Vol. 12 No. 2 (2023): JAFH Vol. 12 No. 2 June 2023
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v12i2.35022

Abstract

Collagen is a connective tissue protein that is mostly produced from cattle and pigs. The use of cows and pigs as the manufacture of collagen causes a disease case, among others are bovine spongiform encephalopathy and transmissible spongiform disease. One of the alternative materials in the isolation of collagen is the use of fish skin. The purpose of this study was to determine the effect of the concentration of acetic acid solution on the characteristics of red snapper collagen produced by the hydroextraction method and to determine the optimum concentration of acetic acid solution in the red snapper skin collagen isolation process using the hydroextraction method. This study was experimental with Completely Randomized Design (CRD) consisting of 3 acetic acid treatment: (P1) acetic acid with concentration 0,1 M; (P2) 0,15 M and (P3) 0,2 M. This study showed that the use of different acetic acid had a significant effect (p<0.05) on the yield parameters that is 1.72 – 2.46%, water content 11.12 – 12.8%, and protein content 83.66 – 84.81% and had no significant effect on the pH. The best treatment was P3 which use the higher concentration of acetic acid that produced higher yield 2.46% and protein content of 84.81%. P3 with concentration of acetic acid 0,2 M can be used for found the best result of characterization of collagen.
Daya Antibakteri Ekstrak Daun Api-Api (Avicennia alba) terhadap Vibrio harveyi Penyebab Vibriosis secara Invitro [In Vitro Antibacteria Activity of Api-Api (Avicennia alba) Leave Extract Against Vibrio harveyi Causes Vibriosis] Zurica Melati Fitri; Kismiyati Kismiyati; Ahmad Shofy Mubarak
Jurnal Ilmiah Perikanan dan Kelautan Vol. 10 No. 2 (2018): Jurnal ilmiah perikanan dan kelautan
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v10i2.10527

Abstract

AbstrakBudidaya udang dihadapkan pada berbagai kendala penyakit yang timbul dan  dapat menyebabkan kematian masal pada larva udang windu, salah satunya  vibriosis yang   disebabkan oleh bakteri Vibrio harveyi.   Zat antibakteri yaitu : flavonoid, saponin dan tanin terbukti dapat membunuh beberapa bakteri patogen.  Daun api – api Avicennia alba mengandung zat anti bakteri sehingga berpotensi digunakan untuk membunuh bakteri Vibrio harveyi. Penelitian ini bertujuan untuk mengetahui konsentrasi optimum ekstrak daun api-api (Avicennia alba) dalam menghambat dan membunuh pertumbuhan bakteri Vibrio harveyi. Penelitian ini  mengunakan metode eksperimental dengan  perlakuan konsentrasi ekstrak daun api-api (Avicennia alba) sebesar  100%, 90% 80%, 70%, 60%, 50%, 40%. 30%, 20%, 10%, 0%.  Parameter yang diamati  dalam penelitian ini  adalah Minimum Inhibitory Concentration (MIC) dan pertumbuhan koloni  pada uji Minimum Bacteria Concentration (MBC).  Hasil penelitian menunjukan bahwa pada  konsentrasi 60% ekstrak daun Avicennia alba dapat menghambat pertumbuhan  V.harveyi. Sedangkan pada konsentrasi 90% ekstrak daun Avicennia alba menyebabkan  bakteri V.harveyi terbunuh.   AbstractShrimp culture has various obstacles such as the emergence of diseases that can cause deaths in tiger shrimp larvae such as vibriosis caused by Vibrio harveyi. Some antibacterial substances such as flavonoid, saponin, and tannin inhibit and kill several pathogenic bacteria. Avicennia alba leaves contain anti-bacterial substances that have the potential to be used to kill Vibrio harveyi bacteria. This study aimed to determine the minimum concentration of Avicennia alba leaves extract for inhibiting and kill the growth of Vibrio harveyi. This study used an experimental method, using  Avicennia alba leaves extract concentration treatment of 100%, 90% 80%, 70%, 60%, 50%, 40%. 30%, 20%, 10%, 0%. The parameters observed in this study were the Minimum Inhibitory Concentration (MIC) and bacteria colony growth in Minimum Bacteria Concentration (MBC) test. The results showed that at a concentration of 60% Avicennia alba leaves extract could inhibit the growth of V.harveyi. While at a concentration of 90% Avicennia alba leaves extract, Vibrio harveyi bacteria was killed. 
Pengaruh Pemberian Pupuk Cair Limbah Ikan Lemuru (Sardinella sp.) dengan Dosis yang Berbeda Terhadap Pertumbuhan Chlorella sp. [Effect of Liquit Fertilizer Waste Sardinella Fish (Sardinella sp.) With Different Doses To The Chlorella sp. Growth] A. Shofy Mubarak; Diana Meritasari; Laksmi Sulmartiwi; Endang Dewi Masithah
Jurnal Ilmiah Perikanan dan Kelautan Vol. 4 No. 1 (2012): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v4i1.11579

Abstract

Abstract Chlorella sp. can growth in various media that contains enough nutrients, such as N, P, K and other micro-elements. Growth of Chlorella sp. requires the availability of nutrients that can be derived from the chemical decomposition and solution results or waste. Liquid fertilizer waste sardinella fish (Sardinella sp.) is wasted and without any economic value is a new breakthrough in utilizing any part in the fisheries sector so as to maximize the potential of fisheries. Utilization of this, one of which is to make Liquid fertilizer waste sardinella fish (Sardinella sp.) which serve as nutrients for the growth of Chlorella sp. with 7 treatments and repeated 3 times. The treatment used is the doses of liquid fertilizer waste sardinella fish (Sardinella sp.) differently, namely A (0,25 ml/L), B (0,5 ml/L), C (1,75 ml/L), D (1 ml/L), E (1,25 ml/L), F (1,5 ml/L), G (Walne), H (without fertilizer). The results showed that the addition of liquid fertilizer waste sardinella fish (Sardinella sp.) with different doses give a real impact (p<0,05) for growth population of Chlorella sp. The best doses of liquid fertilizer waste sardinella fish (Sardinella sp.) for the population growth rate of Chlorella sp. was 0.75 ml / L is the treatment of C with the highest population of 3500x103 cells / ml.
Pengaruh Konsentrasi Pupuk Azolla pinata Terhadap Pertumbuhan Populasi Spirulina platensis [The Effect Of Azolla pinata Fertilizer Concentration In Spirulina platensis Population Growth ] Masithah Dewi Endang; Faricha Risma Nurani; A. Shofy Mubarak
Jurnal Ilmiah Perikanan dan Kelautan Vol. 4 No. 1 (2012): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v4i1.11581

Abstract

Abstract Spirulina merupakan salah satu jenis alga yang sangat diminati oleh produsen pembenihan dan suplemen kesehatan. Hal yang dapat mendorong peningkatan produksi Spirulina adalah peningkatkan pertumbuhan, yaitu meningkatkan jumlah sel. Salah satu cara untuk meningkatkan pertumbuhan fitoplankton adalah mengontrol kandungan nutrien baik makro maupun mikro pada lingkungan budidaya. Azolla pinata memiliki berbagai unsur hara antara lain N, P, K, Ca, S, Mg, Mn, Fe, Zn, Co. Kandungan unsur kimia dalam Azolla pinata secara kualitatif dan kuantitatif dapat memenuhi kebutuhan unsur makro dan mikro pertumbuhan Spirulina platensis. Azolla pinata merupakan tanaman paku mini yang bersimbiosis dengan cyanobacteria pemfiksasi N2 yaitu Anabaena azollae. Simbiosis ini menyebabkan Azolla pinata sebagai sumber N. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh konsentrasi pupuk Azolla pinata terhadap pertumbuhan populasi Spirulina platensis dan untuk mengetahui konsentrasi optimal pupuk Azolla pinata yang dapat menghasilkan pertumbuhan tertinggi Spirulina platensis. Penelitian dilaksanakan di Fakultas Perikanan dan Kelautan Universitas Airlangga Surabaya. Rancangan penelitian yang digunakan adalah Rancangan Acak Lengkap (RAL) dilanjutkan dengan uji jarak berganda Duncan. Bahan uji yang digunakan dalam penelitian ini adalah S. platensis yang dikultur pada botol kaca 500 ml dengan lima perlakuan dan empat kali ulangan. Bahan pupuk yang digunakan adalah 500 gram Azolla pinata dan 2 liter aquades. Konsentrasi pupuk Azolla pinata yang diberikan pada perlakuan adalah A (3,5 ml), B (5,5 ml), C (7,5 ml), D (9,5 ml), E (kontrol Walne 1 ml). Parameter utama yang diamati adalah populasi, sedangkan parameter pendukung yang diamati adalah suhu, pH, dan salinitas. Hasil penelitian menunjukkan bahwa konsentrasi pupuk Azolla pinata memberikan pengaruh yang berbeda nyata terhadap pertumbuhan populasi Spirulina platensis. Penambahan pupuk Azolla pinata kedalam media kultur menggunakan konsentrasi 3,5 ml menghasilkan populasi Spirulina platensis tertinggi sebesar 1708,6 x 103 unit/ml.
Korelasi Perubahan Garis Pantai Terhadap Konservasi Penyu Hijau (Chelonia mydas) Di Taman Nasional Meru Betiri, Jawa Timur [Coast Line Changes Correlation On Green Turtle (Chelonia mydas) Consetvation At Meru Betiri National Park, East Java] Sapto Andriyono; A. Shofy Mubarak
Jurnal Ilmiah Perikanan dan Kelautan Vol. 3 No. 2 (2011): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v3i2.11598

Abstract

Abstract Varity of the effect of climate change including of beach disturbance on the coastal area. Beach disturbance also happen on Sukamade beach at Maru Betiri National Park (TNMB). These areas as nesting site of sea turtle are usually visiting by Green turtle (Chelonia mydas) and others sea turtle species. This research conducted to understanding of relationship between beach area and green turtle (Chelonia mydas) conservation activity was done at October 2010. Survey used to collecting primary data, and secondary data collecting from National Park Office. The result show that beach disturbance affected to the nesting activity of green turtle (Chelonia mydas). Formula show that interaction between beach extensive and number sea turtle are nesting is y = 1.4943x + 417.13 (r:2.46%). In the other hand, beach extensive and number of nest was find out the regretion formula is y = 2.3615x -656.45 (r = 20.66%). Physic and chemistry parameter in aquatic and sea turtle nest on the range are toleranced for conservation activity of green turtle. Physic and chemistry parameter in aquatic are pH 8-8.2, salinity 29-32o/oo , Light intensity 8250-8342 lux, sand pH 6,6 -8.5, sand humidity 8-8.5 and Amonia-Amonium 0.00 – 0.5 mg/l. Disolved oxsigen measuring on 6.027 – 7.627 mg/l
Pengaruh Luas Penutupan Terumbu Karang Pada Lokasi Biorock dan Reef Seen Terhadap Keragaman Spesies Ikan di Wilayah Perairan Pemuteran, Bali [Closure Area Effect On Reefs Rehabilitation in Biorock and Reef Seen Habitat Against Fish Species Diversity In Regional Aquatic Pemuteran, Bali ] A. Shofy Mubarak; Destya Twinandia; Akhmad Taufiq Mukti
Jurnal Ilmiah Perikanan dan Kelautan Vol. 3 No. 2 (2011): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v3i2.11600

Abstract

Abstract This study aims to determine the percentage of coral reefs with extensive closure Biorock method and determine the relationship between the percentage of closure on the abundance of fish species in the area of rehabilitation with Biorock method. The study was conducted at two locations, locations with Biorock and natural reefs (Reef Seen). Research carried out by using the line transect method or Point Intercept Transect (PIT) with a direct enumeration technique Cencus Visual Method (VCM) and photo transects for data reef fish and coral reef data. Transects installed in locations that have been selected, the depth of 8 m. The main parameters studied, namely the abundance of coral and fish species identified at the sites. Supporting the studied parameters of physical and chemical parameters of waters, including: temperature, salinity, water pH and brightness and the percentage cover of coral. Measurement of water quality, including: temperature, depth, salinity, acidity and brightness. The results of this research is vast percentage of coral cover on the location Biorock of 38.5% with the type of branching Acropora growth, while at the location of Reef Seen by 43.5% to the type of hard coral growth form of corals that dominate submasif. The percentage of coral cover has no influence on the abundance of species of reef fish in the territorial waters of Pemuteran, Bali.
Pengaruh Penambahan Pupuk Bintil Akar Kacang Tanah Sebagai Sumber Nitrogen Dan Fosfor Terhadap Populasi Chlorella sp. [The Effect Of Addition Fertilizer Roots Nodule Peanut As A Source Of Nitrogen And Phosphorus To The Population Of Chlorella sp. ] Rr. Juni Triastuti; A. Shofy Mubarak; Likanimasayu Prabandari
Jurnal Ilmiah Perikanan dan Kelautan Vol. 3 No. 2 (2011): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v3i2.11601

Abstract

Abstract Chlorella sp. is one natural food that widely used in hatchery fish, shrimp and oyster. The availability of natural food is a factor that has an important role in farming activities. Culture of Chlorella sp. generally use technical fertilizer (Walne) where nitrogen and phosporus in Walne beginning chemist fertilizer the price expensive. One source of nitrogen and phosphorus naturally obtained from the roots of peanuts. The root nodules contained peanut-containing nodule bacteria Rhizobium are able to bind nitrogen from the air element. By doing immersion can be obtained nutrients to the roots of peanuts are nitrogen and phosphorus that is soluble in water. So that the root nodules of peanuts can be used as a source of nitrogen and phosphorus to increase the population of Chlorella sp. The purpose of this study was to determine the effect of the addition of fertilizer and the concentration of peanut root nodules as a source of nitrogen and phosphorus to the population of Chlorella sp. The research was conducted in June until July in the Laboratory of Education Faculty of Fisheries and Marine Fisheries, Airlangga University, Surabaya. The study design used was Completely Randomized Design (CRD). Materials tested in this study was Chlorella sp. whereas fertilizer used is the root nodules of peanut fertilizer and manure Walne. The concentration of the addition of fertilizer peanut nodule is the treatment A (2.25 ppm), treatment B (4.5 ppm), treatment C (9 ppm) and treatment D (18 ppm). Control treatments using fertilizer Walne 0.5 ml/l (control 1) and 1 ml/l (control 2). The main parameter is observed population density, while supporters of the observed parameter is the measurement of temperature, pH and salinity. The results showed that the addition of fertilizer peanut nodule as a source nitrogen and phosporus influence population of Chlorella sp. The addition of fertilizer best peanut nodule is the treatment B of 1,43755x106 cells/ml on the third day. Water quality parameters during the study remained within the tolerance limit for the growth of Chlorella sp. is pH 7-8, room temperature 29-32 0C range, salinity range between 28-40 ppt and water temperature ranges between 28-30 0C.
IbM Kelompok Petambak Tradisional Udang Windu Di Desa Kedung Peluk, Yang Menghadapi Penurunan Hasil Panen Secara Drastis Sejak Tahun 1994 [IbM For Windu Shrimp Small Scale Farmer Traditional In Kedung Peluk Village Whose Gets Harvesting Decreased Since 1994 ] Gunanti Mahasri; A. Shofy Mubarak; Sudarno Sudarno
Jurnal Ilmiah Perikanan dan Kelautan Vol. 3 No. 2 (2011): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v3i2.11603

Abstract

Abstract Tiger shrimp (Penaeus monodon Fab) is one of the economically important shrimp, until 1992 became the most important of non petroleum export commodity from fishery sector. Since the end of 1993 up to now, the Penaeus monodon Fab death level has been relatively high and due to this circumstance have been caused many ponds collapsed so that the shrimp production was dramatically declined for year by year. Candi District is one of the Sidoarjo Region areas which have big fisheries potential, aspecially for the breakist water pond, that the topest as the other district. There are a lot of shrimp dead cases until now. But, so that 80% of breakist water pond were broken and not operational. The objective of this societies service activities is applicated a new shrimp culture technology with traditional plus Imuno-Biocirculation System. Imuno-Biocirculation System (SIBR) for increases the shrimp harvest at Candi District Region of Sidoarjo, at June until November 2011. The method using in the activity were socialitation/counseling, dempond and guiding to application of the SIBR model in one periode. Monitoring and evaluation about this result were done in one month after the activity ending. This result showed that a positive indication. There was the knowledges of the farmer in ceases by socialization, it also applicated a model in the right method for shrimp culture. There were also showed that the SIBR model can in ceased the shrimp harvest from 272,43 kg/ha to 854,66 kg/ha, it means was increased 313%. The conclution of this activity is the SIBR model can increased the shrimp harvest and can applicates in more larges area in Sidoarjo Region.
Pengaruh Pemupukan Ulang Kotoran Ayam Kering Terhadap Populasi Cacing Tubifex tubifex [The Effect Of Remanuring Dry Chicken Manure In Tubifex tubifex Population] Kustiawan Tri Pursetyo; Woro Hastuti Satyantini; A. Shofy Mubarak
Jurnal Ilmiah Perikanan dan Kelautan Vol. 3 No. 2 (2011): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v3i2.11604

Abstract

Abstract Tubifex tubifex is one kind of natural food for fish larva. This worm has an important role because it can support the fish growth faster than another natural food like Daphnia sp. or Moina sp. It is caused by its has high nutrition. Dry chicken manure can be used for manuring worm media because it have high organic matter. The purpose of this research was to know the effect of remanuring dry chicken manure to the population of Tubifex tubifex and to know the remanuring of dry chicken manure which is gives high population of Tubifex tubifex. The method used was experimental method. Completely Randomize Design was used in this research with 4 dose manuring treatments and 4 replication : PI (dose 0,08 g/cm2/l days), P2 (dose 0,4 g/cm2/5 days), P3 (dose 0,8 g/cm2/10 days) dan P4 (dose 1,2 g/cm2/15 days). The analysis result showed that the treatment of PI (dose 0,08 g/cm2/l day), manuring of dry chicken manure could give the highest population of Tubifex tubifex was 505 individuals reached at days20. At days-30 there was decreasing total number population so that the harvesting of Tubifex tubifex could be done at days-20. The water quality during this research were pH 6,4 - 7; DO 1,2 - 3,8 mg/1; water temperature 28 - 30 °C and ammonia 0,06 mg/1. The water quality was in optimum condition for Tubifex tubifex growth.
Pengaruh Konsentrasi Air Kelapa Muda dan Madu dalam NaCl Fisiologis terhadap Motilitas dan Lama Hidup Spermatozoa Ikan Patin (Pangasius pangasius) [The Effect of Concentration Young Coconut Water and Honey in 0,9% Sodium Chloride to Motility and Life Time Catfish (Pangasius pangasius) Spermatozoa] Laksmi Sulmartiwi; Eka Ainurrohmah; A. Shofy Mubarak
Jurnal Ilmiah Perikanan dan Kelautan Vol. 3 No. 1 (2011): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v3i1.11626

Abstract

Abstract Ability nature maturation of catfish just one time a year at early rainy season (November-March) and nature seed population was down because catching, so to solve the probleme is storage of catfish spermatozoa and can used as need. Young coconut water and honey as thinner in storage catfish spermatozoa content glucose and fructose result ATP as energy resources. Destination of this study to know effect of young coconut water and honey to catfish spermatozoa motility and live time in storage process. Study method is experiment with Completely Randomization Design as experimental design. With different treatment young coconut water is A (0%), B (19,4%), C (39,4%), D (59,4%), E (79,4%) and F (99,4%), and add 0,6% honey at every treatment. Save the treatment at 5-7°C for 36 hours with checked every 4 hours. Data analyzed with Anova and continued by Duncan's Multiple Range Test. Result this study shown used young coconut water with different concentration is not different significant at motility but different significant at live time catfish spermatozoa. Young coconut water with concentration 99,4% and honey 0,6% influence at catfish spermatozoa live time. 
Co-Authors A. Taufiq Mukti A.Taufiq Mukti Abdillah, Annur Ahadi Adde Ermawan Adriana Monica Sahidu Akhmad Taufiq Mukti Akhsan Panna Alfi Hermawati W Amilaningrum, Diadara Amin, Muhamad Nur Ghoyatul Annisa Nur Rohmah Purnamasari Apriana Vinasyiam Arum, Kartika Dwi Sekar Boedi Setya Rahardja Browijoyo Santanamurti Cahyani Prasetyawati, Fatimah Carattri, Money DARMAWAN SETIA BUDI DEDI JUSADI Desi Nawang Purnamasari Destya Twinandia Diah Ayu Satyari U Diah Trie Ridyaning Tias Diana Meritasari Djamil, Hasim Dwi Yuli Pujiastuti Eka Ainurrohmah Eka Saputra Eka Saputra Endang Dewi Masithah Endang Tri Wahyurini Faricha Risma Nurani Fasya, Arif Habib Fitrawan, M. Dzaky Fransisca, Fida Gunanti Mahasri Heru Pramono Husein, Mohamad Akmal Alwi Intan Cahyarini Isroni, Wahyu Juanda Juanda Julie Ekasari Kamiliya Zahrah Taher Kenconojati, Hapsari Kismiyati , Laksmi Sulmartiwi Layuk, Tresia Sanda Likanimasayu Prabandari M. Zairin Junior Maulida Rosa Umainana Maulidia, Cici Mayangsari, Cholivia Melani Indah Sari Manik MUHAMMAD AGUS SUPRAYUDI Muhammad Daffa Al Rasyid Nurhayati Müller, Tamás Mulyono Mulyono Munjayana, Munjayana N. Juni Triastuti Ninin C Nurmalia Dewi, Nina Patmawati Patmawati, Patmawati Permana Sulistyo Rini Pursetyo, Kustiawan tri Puspitasari, Febriyanti Putri Alia Paramitha Putri, Monica Angelina R. Rahardyan Prasetyo Rahayu Kusdarwati Rahmadani Rahmadani Ramadhan, Novelix Arditan Ratna Diyah Palupi Reni Oktaviani Restanti, Maya Rochmatika, Endah Rohmatin, Aulia Rokhim, Akhmad Nur Rr. Juni Triastuti Sapto Andriyono Saragih, Friska Setiawani Sari, Devi Elvina Septuresty Hartri Eka Setyawati Sigit Sihombing, Agusmanto Steffani Lutfi Yuni firdausi Suciyono, Suciyono Sudarno, Sudarno Utari, Heny Budi Veryl Hasan Widigdo, Syafrianur Win Darmanto Wiyoto Wiyoto, Wiyoto Woro H. Satyantini Woro Hastuti Satyantini Woro Hastuti Setyantini Wulan, Novrida Yeni Dhamayanti Yuli Pujiastuti, Dwi Yusuf Taufik Hidayat Zurica Melati Fitri