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All Journal Jurnal Veteriner JOURNAL OF COASTAL DEVELOPMENT ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Perikanan dan Kelautan Berkala Perikanan Terubuk Jurnal Ilmu Lingkungan Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Jurnal Perikanan dan Kelautan Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Asian Journal of Aquatic Sciences Unri Conference Series: Community Engagement Journal of Coastal and Ocean Sciences Journal of Rural and Urban Community Empowerment Abdimas Indonesian Journal Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Jurnal Perikanan dan Kelautan Jurnal Natur Indonesia

Nursyirwani, Nursyirwani

Universitas Riau

Author-ID : 3294310

Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Physics Social Sciences Veterinary Other

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Daya Hambat Ekstrak Kuda Laut (Hippocampus spinosissimus) terhadap Pertumbuhan Bakteri Vibrio alginolyticus Panjaitan, Grace P; Yoswaty, Dessy; Nursyirwani, Nursyirwani
Jurnal Perikanan dan Kelautan Vol 22, No 2 (2017)
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.22.2.18-23

Tujuan dari penelitian ini adalah menguji daya hambat ekstrak kuda laut(Hippocampus spinosissimus) terhadap pertumbuhan bakteri Vibrio alginolyticus.Penelitian ini dilakukan pada bulan Desember 2016 sampai Januari 2017 di LaboratoriumMikrobiologi Laut Jurusan Ilmu Kelautan, Universitas Riau. Ekstrak etanol kudalaut memiliki kandungan kimia steroid/triterpenoid, saponin dan glikosida sertamemiliki efek sitotoksik Uji daya hambat dilakukan dengan metode eksperimen menggunakanempat konsentrasi ekstrak (100%, 50%, 25% dan 12,5%). Dari hasil penelitianyang dilakukan ditemukan bahwa rata-rata diameter zona daya hambat ekstrakkuda laut tertinggi terdapat pada konsentrasi 100% (3,67 mm) sedangkan yang terendahterdapat pada konsentrasi 25% (0,73%). Ekstrak kuda laut mampu menghambatpertumbuhan bakteri V. alginolyticus dengan potensi lemah dengan nilai rata-rata diameterzona daya hambat <5 mm. Ekstrak kuda laut cenderung bersifat bakteriostatik.

TOLERANCE OF PROBIOTIC BACTERIAL CANDIDATE FROM KAKAP PUTIH ON pH AND BILE SALT Harjuni, Fiki; ', Nursyirwani; Mulyadi, Aras
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 3, No 2 (2016): Wisuda Oktober Tahun 2016
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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The use of probiotic is one of alternatives to solve disease problem in aquaculture. This research aimed to examine tolerance of three lactic acid bacteria (KP1, KP2, and KP3) isolated from kakap putih (Lates calcarifer) toward pH and bile salt at different consentration. The bacterial isolates were enriched on MRS agar medium. Tolerance to pH was conducted in MRS broth at pH 3, 5 and 8. While, tolerance to bile salt were performed on MRS agar at consentration of 0, 0,1, 0,2, 0,3, and 0,4 %. Isolate KP3 at pH and incubated for 48 hours showed the highest tolerance, but the highest tolerance to bile salt was indicated by isolated KP2 at bile salt consentration of 0,3 %.Keyword: Tolerance, probiotic, Kakap Putih, pH, Bile Salt.

Supplementation of Lactic Acid Bacteria in Feed Induced Non-Specific Immune Response of Tiger Grouper (SUPLEMENTASI BAKTERI ASAM LAKTAT PADA PAKAN MERANGSANG TANGGAP KEBAL NON SPESIFIK IKAN KERAPU MACAN) Nursyirwani .; Widya Asmara; Agnesia Endamg Tri Hastuti Wahyuni; Triyanto .
Jurnal Veteriner Vol 14 No 3 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (270.773 KB)

Lactic acid bacteria (LAB) have an ability to enhance innate responses in fish. The aim of this studywas to study the effect of three LAB isolates (Kerapu Situbondo Usus: KSBU 12C, KSBU 5Da, and KSBU9 on non-specific immune responses of tiger grouper (Epinephelus fuscoguttatus). The fish were fed dietsupplemented with each of the bacterial isolates at final concentration of 108 CFU mL-1 twice every day.The haematology parameters (haematocrit, leucocyte count, heterophils/neutrophils, monocytes,lymphocytes), phagocytic activity and total LAB were measured every seven days. After 28 days, the fishwere challenged with Vibrio alginolyticus. During the feeding period, the haematocrit values of fish fed withLAB isolates were higher than those of control group, and values ranged from 24.67±8.33 % to 40.50±7.09%. The leucocyte counts increased, mainly after being challenged with V. alginolyticus, and the valuesranged from 40.33 ±6.03 × 1000 cell mL-1 to 139.33±15.57 × 1000 cell mL-1. With the exception oflymphocytes, proportion of heterophils and monocytes, and phagocytic activity were higher in fish fed withLAB isolates than those of control. The presence of LAB in the gastrointestinal tract of fish fed with LABresulted in higher haematology parameters and phagocytic activity in comparison to fish fed control diet.In conclusion, supplementation of LAB in diet could enhance non-specific immune response of tiger grouper.

COMPARISON OF ESCHERICHIA COLI CONCENTRATION BETWEEN BENGKALIS COASTAL WATERS AND ESTUARY BANTAN TENGAH RIVER Nursyirwani .; B.A. Moestomo
JOURNAL OF COASTAL DEVELOPMENT Vol 6, No 1 (2002): Volume 6, Number 1, Year 2002
Publisher : JOURNAL OF COASTAL DEVELOPMENT

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (206.663 KB)

Bengkalis coastal waters and estuary Bantan Tengah River have been evaluated for bacteriological pollution level from November 2000 to April 2001. The objective was to compare Escherichia coli concentration as an indicator organism in the two ecosystems. The results indicated that E. coli concentration was higher in Bengkalis coastal waters than at estuary Bantan Tengah River, either at spring or at ebb tides. E. coli concentration, in Bengkalis coastal waters was higher at spring tide (993 cfu/100 ml) than that at ebb tide (775 cfu/100 ml). On the contrary, the E. coli concentration in estuary Bantan Tengah River was higher at ebb tide (247 cfu/100 ml) than at spring tide (22 cfu/100 ml).

Properti Probiotik Isolat Bakteri Asam Laktat untuk Mengendalikan Pertumbuhan Vibrio alginolyticus pada Ikan Kerapu Macan (Epinephelus fuscoguttatus) Nursyirwani Nursyirwani; W. Asmara; A.E.T.H. Wahyuni; Triyanto Triyanto
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 16, No 3 (2011): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Tujuan penelitian ini adalah untuk menyeleksi isolat bakteri asam laktat (BAL) yang telah diisolasi dari ikan Kerapu Macan (Epinephelus fuscoguttatus) berdasarkan toleransi terhadap pH dan bile salts, patogenisitas dan kemampuannya menekan pertumbuhan Vibrio alginolyticus pada uji ko-kultur. Hasil penelitian menunjukkan bahwa dua puluh isolat BAL yang telah diuji antagonismenya terhadap V. alginolyticus pada penelitian terdahulu, dapat hidup pada pH rendah 3 dan tumbuh pada konsentrasi bile salts 0,5% kecuali isolat KSBU 13D. Semua isolat tidak patogen terhadap ikan Kerapu Macan setelah diinfeksi BAL pada konsentrasi 107 cfu/ml secara intraperitoneal (IP). Pada uji ko-kultur, dari 6 isolat BAL yang dipilih, tiga isolat (KSBU 12C, KSBU 5Da, KSBU 9) berturut-turut mampu menekan pertumbuhan V. alginolyticus, dan penambahan konsentrasi BAL terbaik adalah 107 cfu/ml. Kata kunci: bakteri asam laktat, vibriosis, patogenisitas, ko-kultur, kerapu macan The research objective was to select lactic acid bacteria which previously isolated from Tiger Grouper (Epinephelus fuscoguttatus) based on pH and bile salts tolerances, pathogenicity test and ability to decrease the growth of Vibrio alginolyticus in co-culture test. The result shows that twenty isolates which had also been examined their antagonisms against V. alginolyticus previously, were able to survive relatively low pH values 3 and able to survive at high bile salts concentrations 0.5% except KSBU 13D isolate. All isolates were nonpathogenic to Tiger Grouper after being intraperitoneally infected with the lactic acid bacteria at concentration 107 cfu/ml. In co-culture test, V. alginolyticus was reduced significantly by three isolates (KSBU 12C, KSBU 5Da, KSBU 9) from 6 isolates selected, and the best additional bacterial concentration was 107 cfu/ml. Key words: lactic acid bacteria, vibriosis, pathogenicity, kerapu macan

Isolasi Bakteri Asam Laktat dari Usus Ikan Kerapu Macan (Epinephelus fuscoguttatus) dan Potensinya Sebagai Antivibrio Nursyirwani Nursyirwani; W. Asmara; A.E.T.H. Wahyuni; Triyanto Triyanto
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 16, No 2 (2011): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Tujuan penelitian ini adalah untuk mengisolasi dan menguji potensi antibakterial bakteri asam laktat dari ikan Kerapu Macan (Epinephelus fuscoguttatus) terhadap bakteri patogen Vibrio alginolyticus. Bakteri tersebut diisolasi dari usus ikan dengan metode sebarulas pada media agar MRS dan GYP+CaCO3. Koloni yang tumbuh diidentifikasi berdasarkan karakter morfologi, biokimia dan fisiologi. Aktivitas antibacterial terhadap V. alginolyticus diuji dengan metode difusi kertas cakram pada media Zobell 2216E lapis ganda, diameter zona jernih disekitar kertas cakram diukur dengan kaliper. Pada medium agar GYP+CaCO3 didapatkan 21 isolat yang menampakkan zona jernih disekitar koloninya. Dari karakteristik morfologi, biokimia dan fisiologi dari isolat-isolat tersebut semua isolat dapat dikategorikan kedalam genus Lactobacillus. Tetapi, dari uji aktivitas antivibrio hanya 20 isolat menunjukkan zona hambat yang berkisar dari 14,0-21,5 mm, dimana tiga isolat menunjukkan aktivitas tertinggi, yaitu berturut-turut isolat KSBU 12C, KSBU 13D dan KSBU 5Da.Kata kunci: Isolasi, bakteri asam laktat, antivibrio, Kerapu Macan The objectives of this research were to isolate and examine antibacterial potency of lactic acid bacteria from Tiger Grouper (Epinephelus fuscoguttatus) against pathogen, Vibrio alginolyticus. The bacteria were isolated from the fish intestine by spread plate method on MRS and GYP+CaCO3 agar media. The grown colonies were identified based on morphological, biochemical and physiological characters. Antibacterial activity against V. alginolyticus was examined by the paper disc diffusion method on Zobell 2216E double layer agar, and clear zone diameter around the paper disc was measured by using caliper. Twenty one isolates with clear zone around the colonies were obtained from the GYP+CaCO3 agar. Morphological, biochemical and physiological characteristics of the colonies and cells indicated that all isolates might be categorized into Lactobacillus. However, there were only twenty isolates showed inhibition zones from 14.0-21.5 mm in antivibrio activity test, of which the highest activity was indicated by three isolates namely KSBU12C, KSBU 13D and KSBU 5Da, respectively. Key words: Isolation, lactic acid bacteria, antivibrio, Tiger grouper.

Isolasi dan Karakterisasi Bakteri Hidrokarbonoklastik dari Perairan Dumai dengan Sekuen 16S rDNA Nursyirwani Nursyirwani; Kathy Copper Amolle
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 12, No 1 (2007): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Penelitian ini dilaksanakan dari bulan September sampai Desember 2006. Tujuan penelitian adalah untuk mempelajari karakteristik molekuler bakteri hidrokarbonuklastik yang diisolasi dari perairan laut Dumai berdasarkan sekuen 16S rDNA. Isolasi bakteri dilakukan pada media cair dan padayang ditambahk an Sumatran crude oil. Karakteristik molekuler diperoleh melalui isolasi dan ampHdkasi DNA dengan PCR dan sekuensing menggunakan ABI 3130 Genetic Analyzer. Dari 6 isolat yang danalisis, hanya ada 3 isolatyang dapat disekuensing. Dari perbandingan dengan BLAST database, didapatkan kesamaan sekuen yang terdekat untuk isolat CA (91%) adalah Providenda vermicola, isolat DA (93%) adalah Burkholderia cepacia, dan isolat FA (99%) adalah Myroides odoratimimus.Kata kunci: Bakteri hidrokarbonuklastik, PCR dan 16S rDNAThe research was conducted from September to December 2006. The aim was to study molecular characterization of hidrocarbonoclastic bacteria based on sequence 16S rDNA from Dumai waters. The bacteria was isolated in both broth and solid media added with the Sumatran crude oil. Molecular characterization included DNA isolation and amplification using PCR, and sequencing by ABI 3130 Genetic Analyzer. Three of six isolateswere successfully sequenced. The comparison of 16S rDNA with known 16S rDNA sequences from BLAST database showed that the closest sequence similarity of isolate CA (91%) was Providencia vermicola, isolate DA(93%) was Burkholderia cepacia, and isolate FA (99%) was Myroides odoratimimus.Key words : Characterization, Hydrocarbonoclastic Bacteria, PCR, 16S rDNA

Multiple Antibiotic Resistance Index of Escherichia coli Isolates from Dumai Sea Waters Riau Province Mardalisa Mardalisa; Feliatra Feliatra; Nursyirwani Nursyirwani
Berkala Perikanan Terubuk Vol 49, No 1 (2021): Februari 2021
Publisher : Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/terubuk.49.1.734-739

The pattern of antibiotic consumption by the community which is carried out independently without medical guidance causes antibiotic pollution has reached sea waters, one of its impacts on the nature of bacterial resistance. This study aims to look at the pattern of antibiotic sensitivity to E coli that isolated from Dumai sea waters. E coli isolates that grown on MHA media were tested based on the Kirby Bauer disk diffusion method. Seventeen isolates were identified, among which a high rate of resistance to penicillin, isoniazid and streptomycin (17), a high rate of resistance to chloramphenicol (5), intermediate susceptibility to chloramphenicol (11) and only 1 sensitive to chloramphenicol. The multiple antibiotic resistance (MAR) index of E coli isolates was 0.82. These show that antibiotics like penicillin, isoniazid, streptomycin and chloramphenicol were not suitable antibiotic for E coli infection treatment. The community is expected to be more disciplined in using antibiotics and to be careful in using the Dumai sea for fishery activities

ANALYSIS OF THE POTENTIAL DEVELOPMENT OF MANGROVE FOREST ECOTOURISM IN KAMPUNG SUNGAI KAYU ARA, SIAK DISTRICT, RIAU PROVINCE Dessy Yoswaty; Irwan Effendi; Nursyirwani Nursyirwani; Joko Samiaji; Muhammad Razali
Berkala Perikanan Terubuk Vol 49, No 1 (2021): Februari 2021
Publisher : Fakultas Perikanan dan Kelautan, Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/terubuk.49.1.775-787

The mangrove forest ecosystem in Sungai Kayu Ara Village, Sungai Apit Subdistrict, Siak Regency, Riau Province has a unique or distinctive appeal, which has the potential to be developed as a mangrove forest ecotourism destination. The research objective was to analyze the potential for developing mangrove forest ecotourism in Sungai Kayu Ara Village in realizing sustainable mangrove forest conservation. The method used in this research is the survey method, which was carried out in May-July 2020. Mangrove density data was taken using the line transect plot technique, which consists of 3 observation transects. Each transect has 3 plots, each plot measuring 10 x 10 m2. The identification results found 6 types of mangroves that grow in the mangrove forest area of Sungai Kayu Ara Village, namely Rhizophora apiculata, Avicennia alba, Bruguiera gymnorrihiza, Sonneratia alba, Nypa fruticans, and Xylocarpus granatum. The mangrove density value is 1234 ind / ha. The average value of the Tourism Suitability Index (IKW) is 76.75%. (S2 category), which is suitable for development as a mangrove forest ecotourism area. Mangrove forest ecotourism development strategies based on SWOT analysis include 1) Increasing stakeholder cooperation for mangrove forest conservation; 2) Developing facilities and infrastructure for the development of mangrove forest ecotourism; 3) Provision of information and promotion of manrove ecotourism through print and electronic media; and 4) Increased quality of skilled and skilled human resources for the development of mangrove forest ecotourism.

ANALISIS DAMPAK LINGKUNGAN BUDIDAYA IKAN DALAM KERAMBA DI WADUK SUNGAI PAKU KECAMATAN KAMPAR KIRI KABUPATEN KAMPAR Yulhadis Yulhadis; Usman M. Tang; Nursyirwani Nursyirwani
Jurnal Ilmu Lingkungan Vol 12, No 1 (2018): Jurnal Ilmu Lingkungan
Publisher : Program Pascasarjana Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jil.12.1.p.1-14

The study was conducted in January-March 2018, field analysis (insitu) was conducted in Sungai Paku Reservoir of Sungai Paku Village Kampar Kiri Sub-district Kampar District Riau Province. Laboratory analysis (exsitu) was conducted in Ecology and Waters Management Faculty of Fishery and Marine Science University of Riau Pekanbaru. The purpose of this research is to analyze the quality of Sungai Paku reservoir to the floating net cage (FNC) activity, to analyze and develop the socialization of FNC management in the Sungai Paku reservoir. This research uses survey method, by conducting direct measurement in the field, conducting analysis and interviews of research location through questionnaire to get more accurate data. To analyze the environment at about 3 different stations over the inlet of the reservoir, within the FNC area, and outlet of the reservoir. Physical chemistry parameters include 95-95.2 cm Brightness, temperature 30-30.3oC, current velocity 3.92 - 3.97, dissolved oxygen 0.98 - 1.00 mg / l, pH 5, Phosphate 0.1850-0.1851 mg / L, nitrate 0.2102-0.2104 mg / l , ammonia 0.0675-0.0677 mg / l. The average abundance of phytoplankton 17,600 - 22,400 cells / ml. Abundance of macrozoobenthos 866 - 1471 Ind / m2. Evaluation of water quality at each observation station indicates that the recording at each station is DO, pH and total P which have not meet the standard of water quality.

Co-Authors ', Afrimansyah A.E.T.H. Wahyuni A.E.T.H. Wahyuni Afrimansyah ' Agnesia Endamg Tri Hastuti Wahyuni Agnesia Endang Tri Hastuti Wahyuni Alfarisy, M. Phyto Alfiati Silfi Andhini, Nadia Andrei Putra Zirma Anisa Qoriman Apriliani Indrawati Putri Aras Mulyadi Arman Effendi Asri, Zainul B.A. Moestomo Bachar, Sutan Barbara, Shanta Batubara, Ummi Mardhiah Bintal Amin BP, Valentina Dian Christine M Situmeang Claudia, Kristiwany Mayneke Darmawan, Ernanda P. Desi Anggreni Dessy Yoswati Dessy Yoswaty Desy Mutia Sari Devi Puspita Dewita Dewita Efriyeldi, Efriyeldi Elfis Suanto Elizal, Elizal Elkana Gabariel Fathoni, Ahmad Yusron Feliatra Feliatra, Feli Feliatra, Pamela Isya Ferdana, Gilang Fiki Harjuni Furqan, M. Alief Gavion Roston Sitepu Gina Ulfa Fitria Gunova, M Taufik Gunova, M. Taufik Gustina, Julia Gustina, Lenny Halima, Asyatul Hanies Ambarsari Hanifa, Zurya Haura, Sari R. Al Ida Rahayu Simanjuntak, Ida Rahayu Iesje Lukistyowati Indri Wahyuni Irvina Nurachmi Irwan Effendi Irwan Irwan Joko Samiaji Julians, Rizky Fajar Kathy Copper Amolle Lazuardi Lazuardi Lazuardi Umar Lestari, Anggi Lola Pitaloka br Barus Mahdiyah, Evfi Mahmudah, Dewi A. Mardalisa, Mardalisa Meiji Nurdiansyah Miptahul Huda Wassyukur Monika, Siska Mubarak Mubarak Mubarak Mubarak Muhammad Hafiz Perdana Muhammad Hafizh Muhammad Razali Mursawal, Asri Musrifin Galib Nainggolan, Robby Netti Aryani Nur Azizah Nuri Aslami Nursyafni Nanggola Oktavia, Dewi Asmiati Pakpahan, Deni Panjaitan, Grace P Povy Hutabarat Pratama, Bima Purba, Paedo Yayaki Puspita, Devi Putra, Rajiv R. Putri Ayu Putri, Redila Putri, Redila Aprilivia Putri, Resty Fauziah Rahmanda, Melysa Ramadhan, Ragiel Rinno Ferryno Roiman Pakpahan Ronal Kurniawan Saharudin Saharudin Sarbaini ' Sari, Desy Mutia Shaumi, Nurul Silitonga, Lamtiur Rotua Sinuraya, Tri Setia Darma Sophia Rahmah Saleha Sri Melinda Citra Sri Wahyuni Sufaidah, Siti Nur Suparmi Suparmi Syafri Syafri Syafruddin Nasution Syahada, Melania Syahril Nedi Syahrul Syahrul Thamrin Thamrin Thamrin Thamrin Tjipto Leksono Triyanto . Triyanto Triyanto Triyanto Triyanto Ummi Mardiah Batubara Usman Muhammad Tang Utami, Rahma I. Valentina Dian W. Asmara W. Asmara Widantari, Meilya Widya Asmara Windarti Windarti Wiya, Siti Ada WULANDARI, APRILIA Yahya Benardus Sihombing Yanti, Della Hijri Yoswati, Dessy Yulhadis Yulhadis Yuni Fitria Yurestira, Ilham Yusni Ikhwan Siregar Zebua, Abdi Hikmat Petra Zevira, Bella Permata Zhilalul Huda

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