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All Journal JOURNAL OF COASTAL DEVELOPMENT ILMU KELAUTAN: Indonesian Journal of Marine Sciences Jurnal Perikanan dan Kelautan Berkala Perikanan Terubuk Jurnal Ilmu Lingkungan Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Jurnal Perikanan dan Kelautan Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Asian Journal of Aquatic Sciences Unri Conference Series: Community Engagement Journal of Coastal and Ocean Sciences Journal of Rural and Urban Community Empowerment Abdimas Indonesian Journal Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan South East Asian Marine Sciences Journal Jurnal Perikanan dan Kelautan Jurnal Natur Indonesia
Nursyirwani, Nursyirwani
Universitas Riau
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Physics Social Sciences Veterinary Other

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ACTIVITY OF HETEROTROPHIC BACTERIA ISOLATED FROM MASJID RIVER, DUMAI, AGAINST PATHOGENIC BACTERIA Zhilalul Huda; Feliatra Feliatra; Nursyirwani Nursyirwani
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 1 Januari s/d Juni 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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                                                ABSTRACT Heterotrophic bacteria is a group of organism that play important role in biogeochemical processes in aquatic ecosystem.The bacteria are able to decompose and remineralize organic materials into a simple inorganic components which is turn to nutrients for phytoplankton, perifiton, and other aquatic microflora. This Study was conducted from September to October 2018. The purpose of this study wos to obtain bacterial isolates that can be used as an antibacterial against pathogenic bacteria and to identify bacteria found in River estuary Masjid Dumai of Riau Province. Based DNA character the result found10 bacterial isolates. The bacterial (1A, 1B, 1C, 1D, 1E, 1F,1J, 2C, 2D, and 2G). These bacteria were able to inhibit the pathogenic growth. DNA identification using PCR and 16S rRN sequence technique resulting found6 types of bacteria, namely Bacillus cereus strain ATCC 14579, B. anthracis strain ATCC 14578 16S,B. methylotrophicus strain CBMB205 16S, B. thuringensis strain YBT-1518, B. pseudomycoides strainNBRC, and B. anthracis strain C10.Keywords: Heterotrophic Bacteria, Antibacteria, 16S rRNA Sequence, Pathogens.
ANALYSIS OF Pb, Cu, and Zn METAL CONNECTIONS IN BLOOD SHELL MEAT (Anadara granosa) WITH DIFFERENT SIZES IN WATERS MUARA ASAHAN RIVER TANJUNGBALAI CITY NORTH SUMATERA Syafri Syafri; Bintal Amin; Nursyirwani Nursyirwani
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 2 Juli s/d Desember 2019
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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ABSTRACT  granosa.This research was conducted from January to April 2019 by taking samples of blood cockles from the waters of the Asahan river, Tanjungbalai city, North Sumatra Province. The purpuse of this research were to know the metal concentrations of Pb, Cu and Zn in different station and different sizes of blood cockle’s shell (Anadara granosa), as well as to find out the level of metal pollution based on MPI (Metal Pollution Index). Analysis of metal concentrations of heavy metal was done by following procedurs outline by Yap et al., (2003). The results showed that the highest Pb metal concentration in meat of small blood clams was found at station 3 namely, 0.7100 μg / g and the low concentration was 0.4716 μg / g at Station 1. While the highest concentration at medium size was found at Station 2 namely , 1.3683 μg / g and low concentration was 0.8000 μg / g at Station 1. The highest Pb concentration at large size was found at Station 3 ie, 2,2200 μg / g and the lowest concentration was 1,2266 μg / g at Station 1. The calculated MPI value for the Asahan estuary research from this study was 3,7260  which was considered quite low compared to other regions. Keywords: Heavy Metal, Pollution, North Sumatra, Industry, Anadara
THE CONDITION OF CORAL REEFS IN THE WATERS OF TIDUNG ISLAND KEPULAUAN SERIBU JAKARTA Muhammad Hafiz Perdana; Thamrin Thamrin; Nursyirwani Nursyirwani
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 1 Januari s/d Juni 2019
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ABSTRACT The aims of this study are to determine the condition of the coral reefs including percentage live coral cover, the diversity index, the uniformity index, and the dominance index of  lifeform coral at waters of Tidung Island Kepulauan Seribu Jakarta. This research has been conducting from 2018 May 7th - 17th at the waters of Tidung Island.The research station was divided into 3 stations based on environmental characteristics with 2 different depths ; 3 and 7 meters. The research station is a coral conservation area, snorkeling area, and natural area. Data of coral reef was obtained by using the Line Intercept Transect (LIT) method. The results showed that the conditions of coral reefs at stations 1 and 2 respectively in the bad category with the percentage of cover area are 3.52% and 10.84%. Whereas at station 3 the condition of coral reefs is in the medium category with the percentage of cover area is 30.01%. The low percentage of coral cover is caused by rising sea water temperatures, port activities and human activities. Water quality such as temperature, salinity, pH, current velocity and brightness at the study site is quite good for coral recovery and growth. Keyword : LIT, Coral Recovery, Diversity, Uniformity, and Dominance Index
Karakteristik Genotip Bakteri Pseudomonas sp yang Diisolasi dari Perairan Laut Dumai Apriliani Indrawati Putri; Felix Feliatra; Nursyirwani Nursyirwani
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 1 Januari s/d Juni 2019
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ABSTRAK Pseudomonas merupakan salah satu bakteri patogen opurtunistik yang menyerang makhluk hidup khususnya manusia dengan sistem kekebalan yang lemah.Tujuan penelitian ini adalah unutuk mengetahui ketahanan bakteri Pseudomonas terhadap antibiotik dan mengetahui kekerabatan antar spesies Pseudomonas sp yang diisolasi dari Perairan Laut Dumai menggunakan analisis 16S rRNA.Penelitian ini dimula dari bulan Januari 2019 – Februari 2019. Dari hasil penelitian Ciprofloxacin paling sensitif dalam menghambat pertumbuhan Pseudomonas dengan zona hambat terbesar 24,00 mm sedangkan yang resisten Sterptomycin dengan zona hambat 8,5 mm dan Erythromycin dengan zona hambat 4,7 mm. Setelah dilakukan analasis sekuensing DNA terhadap ketujuh isolat tersebut, diketahui isolat memiliki kekerabatan dengan bakteri Pseudomonas dengan strain yang berbeda. Tingkat homolog isolat terssebut berkisar 86,01% – 93,01 %.  Isolat P7 FPK Dumai, P21 FPK Dumai, dan isolat P22 FPK Dumai identik dengan Pseudomonas geniculate (89,69%, 93,01% dan 93,00%) sedangkan isolat P9 FPK Dumai identik dengan P. aeruginosa (92,40%). Isolat P1 FPK Dumai identik dengan P. stutzeri (86,16%). Isolat P13 FPK Dumai identik dengan P. monteilii (86,01%) sedangkan P20 FPK Dumai identik dengan P. fluorescens (89,70%) hal ini berarti isolat P7 FPK Dumai, P9 FPK Dumai, P13 FPK Dumai, P20 FPK Dumai dan isolat P1 FPK Dumai merupakan strain baru yang urutan basa dan nitrogennya belum masuk kedalam database sekuen genbank. Sedangkan isolat P21 FPK Dumai dan isolat P22 FPK Dumai mewakili identitas pada tingkat genus.    Kata Kunci : bakteri Pseudomonas, antibiotik, 16S rRNA
ISOLATION OF PROBIOTIC BACTERIA CANDIDATES ISOLATED FROM BARRAMUNDI (Lates calcarifer) ANTAGONISTIC AGAINTS Vibrio alginolyticus Afrimansyah '; Nursyirwani '; Dessy Yoswaty
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 2, No 1 (2015): Wisuda Februari Tahun 2015
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Probiotic is an alternative subtance to prevent fish diseases which is environmentally friendly. The research purpose was to isolate potential probiotic to control bacteria pathogen in barramundi (Lates calcarifer) to control the bacteria pathogen. The probiotic bacteria candidates were isolated from intestine of barramundi by using the spread and streak methods on MRS and TSA media. The antibacterial activity was examinined by using disc diffusion agar method on Zobell 2216E medium. The results showed thirty eight isolates were found which consisted of coccus, white, orange and yellow colonies. Twelve isolates had inhibition against Vibrio alginolyticus,two isolates showed highest inhibition zone and antivibrio activities. The highest inhibition zone (10.04 ± 1,48 mm) and (9,50 ± 2,12 mm) and the antivibrio activities (4.04 mm) and (3.50 mm) were indicated by KPBU2-5 and KPMU2-6 isolates respectively.Keywords: Probiotics, Isolation, Barramundi, Antagonistic.
DEGRADATION CAPABILITY AND PRODUCTION OF BIOSURFACTANTOF CRUIDE OIL-DEGRADING BACTERIA FROM SEDIMENTSELAT BARU Miptahul Huda Wassyukur; Nursyirwani Nursyirwani; Dessy Yoswati
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 1 Januari s/d Juni 2019
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                                          ABSTRACT   Petroleum pollution can originiate from oil spills during drilling, production, refining and transportation activities. One of the hardly to decomposed petroleum contaminants is hydrocarbons, once thehydrocarbons enter the marine environment, the chemical componend can be reduced by biological processes (bioremediation) using bacteria. The purpose of this study was to examine the ability of bacterial isolates from Selat Baru coastal sediments to degrade crude oil and produce biosurfactants. This research was conducted in August to October 2018. The ability of bacterial isolates to degrade oil and biosurfactant production was carried out in the Marine Microbiology Laboratory and Marine Chemistry Laboratory, Department of Marine Sciences, Faculty of Fisheries and Marine, University of Riau. Experimental method was used in this research.. Nine isolates of crude oil degrading bacteria were provided from previous researchers, namely isolates 11p31 (1A), 33p31 (3A), 22p41 (10A), 22p42 (4B), 33p31 (5B), 12p32 (9B), 22p31 (1C), 11p41 (9C), 31p41 (12C). Each isolate was examined the ability to degrade crude oil at  concentration of 1% (0.5 ml), 2% (1 ml) and 3% (1.5 ml). The highest crude oil degradation ability was at 1% concentration by isolates 22p41 (10A) and 11p41 (9C), and for 2% concentrations by isolates 33p31 (5B) and 3% concentrations by bacterial isolates 33p31 (3A). All bacterial isolates were found to produce biosurfactan through the Oil Speading Assay. Emulsifikation test using kerosene indicated kerosene. where the highest emulsification in test 1 is isolate 33p31 (3A). Then in test 2 the highest emulsification value was shown by isolate 33p31 (3A). In Test 3 the highest emulsification value was isolate 11p41 (9C).  Key words:(1)Oil pollution,(2)Degrading,(3)Cruide oil,(4)Biosurfactan,(5)Emulsification  
ABUNDANCE OF Anabaena azollae AND NITRATE LEVELS IN Azolla microphylla BRACKISH WATER CULTURE Nursyafni Nanggola; Irwan Effendi; Nursyirwani Nursyirwani
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 1 Januari s/d Juni 2019
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ABSTRACT               Azolla michrophylla is beneficial for Anabaena azollae because it can act as a host through the supply of oxygen and nitrogen that can be used. Nitrogen fixation carried out by A. Azollae is atmospheric nitrogen gas (N2) converted into ammonia (NH3). This research was conducted at the Laboratory of Marine Microbiology and Marine Chemistry, Department of Mariene Science, Faculty Of Fisheries And Marine Science, University of Riau in April to June 2018. The purpose of this research was to determine the abundance of A. azollae in the growth of A. microphylla plants and determine the levels of nitrate contained in the growth of A. microphylla in brackish water culture. Factorial randomized group design (RAK) was applied in this experimental. Temperature, salinity, and pH were water quality parameters measured. The results showed that the best growth of A. microphylla was obtained at a salinity of 0 ppt with a starter of         A. microphylla 3 gram with a value of 50.42 gram. The highest abundance of A.  azollae is found in salinity 3 ppt with a 3 gram level with the abundance of 5643 cells / ml. A. microphylla and A. azollae had sufficient nitrate levels for their growth in brackish water cultured at 0, 1, 2, and 3 ppt salinitis of in combination with  A. microphylla 1, 1.5 and 3 gram as a starters. The highest nitrate level was obtained on the 14th and 21st day with a value of 1.2625 mg / L and 1.4500 mg / L.   Keywords: Azolla microphylla, Anabaena azollae, Growth, Abundance, Nitrate.
GENOTYPE CHARACTERISTICS OF PATHOGENIC Escherichia coli BACTERIA ISOLATED FROM DUMAI SEA WATER Sophia Rahmah Saleha; Feliatra Feliatra; Nursyirwani Nursyirwani
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 6 (2019): Edisi 1 Januari s/d Juni 2019
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ABSTRACT This research was conducted from March until April 2019. The purpose of this study was to examine the resistance of E. coli bacteria toward antibiotics (ciprofloxasin, streptomycin and erythromycin) and to identify isolated from Dumai seawaters the bacteria genotypes. Resistance test showed that isolated  E1, E4, E6, E8, E11, E13, and E15 were resistant to antibiotic streptomycin and erythromycin with inhibitory zones ranging from 2.9 to 5.9 mm. While sensitivity to ciprofloxasin indicated the inhibition zones of 22.1 mm.  Analysis of E. coli  by using PCR method technique 16S sequences found three strains of bacteria had the highest homologi levels, those were E. coli strain NBRC 102203, E. coli strain JCM 1649 and E. coli strain U 5/41. E1-2 isolate has similarity to E. coli strain NBRC 102203, E4-2 and E6-2 isolates are similar to E. coli strain JCM 1649, E8-2 isolate to strain NBRC 102203, E11-2 isolates to E. coli strain U 5/41, E13-2 and E15-2 isolates to strain NBRC 102203.  Keyword : Escherichia coli, Resistance, Antibiotics, Genotype,  Sekuens 16S rRNA
ISOLATION OF DIESEL OIL DEGRADATION BACTERIA IN THE WATERS AROUND THE PORT OF TELUK BAYUR PADANG CITY Sri Melinda Citra; Dessy Yoswaty; Nursyirwani Nursyirwani
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 5 (2018): Edisi 2 Juli s/d Desember 2018
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ABSTRACT Diesel oil degradation bacteria can be found in various habitats in the marine environment including in the waters around the harbor. This research aims at diesel oil in the waters around the port of Teluk Bayur and test its ability to degrade diesel oil. Isolation of the degrading bacteria has been from April to conductedJune 2018 in the Marine Microbiology Laboratory, Department of Marine Science, Faculty of Fishery and Marine University of Riau.Total count of diesel oil decomposing bacteria ranges from 0.4 x 106 to 7.3 x 106 CFU/ml. Eleven isolates of the diesel oil degradation bacteria were selected, ie BM1a, BM1b, BM1c, BM1d, BM7a, BM7b, BM7c, BM7d, BM14a, BM14c and BM14d. Based on morphological, physiology and biochemical characteristics, seven isolates were irregular, two round and concentric isolates, seven isolates with corrugated colony edges, two irregular and notched isolates, six yellow colony isolates, five white isolates, colony elevation all isolates emerge, all isolates were positive on catalase test, in H2S test all negative isolates, all isolates in the sugar test produced gas, all isolates were motile and Gram negative, all indole isolates were negative, seven methyl red positive isolates and four methy red isolates negative. In the degradation test only six bacterial isolates were selected, ie BM1a, BM1c, BM7a, BM7d, BM14c and BM14d. The highest degradation of diesel oil at concentrations 1%, 2% and 3% were performed by isolates BM1c, BM7a and BM1a respectively. Overall, all isolates were able to degrade diesel oil in the waters around Teluk Bayur Port, Padang City. Keyword: Isolation, Bacteria, Degradation, Diesel Oil 
IS OLATION OF PROBIOTIC CANDIDATE BACTERIA FROM GIANT GOURAMY (Osphronemus gouramy Lac.) INTESTINE TO CONTROL Aeromonas hydrophila Lola Pitaloka br Barus; Iesje Lukistyowati; Nursyirwani '
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 3, No 1 (2016): Wisuda Februari Tahun 2016
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This study aimed to isolate and to find the bacteria that potential as probiotic candidate from Giant Gouramy (Osphronemus gouramy Lac.) intestine cultivated in Riau provincial to be used to control MAS (Motile Aeromonas Septicemia) desease. Survey method and experiment method were conducted in this study. Bacteria was isolated from the fish intestine, and was inoculated on TSA and MRS agar at different pH level (2,4, and 6). The isolateis were indentified by morphological observation, physiological and biochemical test, and by using API 50 CHL test kit. Antibacterial activity of the isolated against Aeromonas hydrophila was tested by the paper disk diffusion method. Eighteen isolates of probiotic candidates were found, and indicated optimal growth in all pH treatment. Based on morphological, physiological test, and biochemichals test, three genus of bacteria were identified as Bacillus sp., Lactobacillus sp., and Bifidobacterium sp. The highest antibacterial activity against A. hydrophila was indicated by Isolate G63 with inhibition zone of 13,2 mm. Based API 50 CHL test kit the isolate was categorized in to Lactobacillus planatarum at similarity level of 84%.Keywords: Giant Gouramy, intestine, isolate, probiotic candidate
Co-Authors ', Afrimansyah A.E.T.H. Wahyuni A.E.T.H. Wahyuni Afrimansyah ' Agnesia Endamg Tri Hastuti Wahyuni Agnesia Endang Tri Hastuti Wahyuni Alfarisy, M. Phyto Alfiati Silfi Andhini, Nadia Andrei Putra Zirma Anisa Qoriman Apriliani Indrawati Putri Aras Mulyadi Arman Effendi Asri, Zainul B.A. Moestomo Bachar, Sutan Barbara, Shanta Batubara, Ummi Mardhiah Bintal Amin BP, Valentina Dian Christine M Situmeang Claudia, Kristiwany Mayneke Darmawan, Ernanda P. Desi Anggreni Dessy Yoswati Dessy Yoswaty Desy Mutia Sari Devi Puspita Dewita Dewita Efriyeldi, Efriyeldi Elfis Suanto Elizal, Elizal Elkana Gabariel Fachry Abda El Rahman Fakhrurozi, Fakhrurozi Fathoni, Ahmad Yusron Feliatra Feliatra, Feli Feliatra, Pamela Isya Ferdana, Gilang Fiki Harjuni Furqan, M. Alief Gavion Roston Sitepu Gina Ulfa Fitria Gunova, M Taufik Gunova, M. Taufik Gustina, Julia Gustina, Lenny Halima, Asyatul Hanies Ambarsari Hanifa, Zurya Haura, Sari R. Al Ida Rahayu Simanjuntak, Ida Rahayu Iesje Lukistyowati Indri Wahyuni Irvina Nurachmi Irwan Effendi Irwan Irwan Joko Samiaji Julians, Rizky Fajar Kathy Copper Amolle Lazuardi Lazuardi Lazuardi Umar Lestari, Anggi Lola Pitaloka br Barus Mahdiyah, Evfi Mahmudah, Dewi A. Mardalisa, Mardalisa Meiji Nurdiansyah Miptahul Huda Wassyukur Monika, Siska Mubarak Mubarak Mubarak Mubarak Muhammad Hafiz Perdana Muhammad Hafizh Muhammad Razali Mursawal, Asri Musrifin Galib Nainggolan, Robby Netti Aryani Nur Azizah Nursyafni Nanggola Oktavia, Dewi Asmiati Pakpahan, Deni Panjaitan, Grace P Povy Hutabarat Pratama, Bima Purba, Paedo Yayaki Puspita, Devi Putra, Rajiv R. Putri Ayu Putri, Redila Putri, Redila Aprilivia Putri, Resty Fauziah Rahmanda, Melysa Ramadhan, Ragiel Rinno Ferryno Roiman Pakpahan Ronal Kurniawan Saharudin Saharudin Sarbaini ' Sari, Desy Mutia Shaumi, Nurul Silitonga, Lamtiur Rotua Sinuraya, Tri Setia Darma Sophia Rahmah Saleha Sri Melinda Citra Sri Wahyuni Sufaidah, Siti Nur Suparmi Suparmi Syafri Syafri Syafruddin Nasution Syahada, Melania Syahril Nedi Syahrul Syahrul Thamrin Thamrin Thamrin Thamrin Tjipto Leksono Triyanto . Triyanto Triyanto Triyanto Triyanto Ummi Mardiah Batubara Usman Muhammad Tang Utami, Rahma I. Valentina Dian W. Asmara W. Asmara Widantari, Meilya Widya Asmara Windarti Windarti Wiya, Siti Ada WULANDARI, APRILIA Yahya Benardus Sihombing Yanti, Della Hijri Yoswati, Dessy Yulhadis Yulhadis Yulinda, Three Yuni Fitria Yurestira, Ilham Yusni Ikhwan Siregar Zebua, Abdi Hikmat Petra Zevira, Bella Permata Zhilalul Huda Zulkifli Zulkifli