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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Bimbingan dan Konseling Jurnal Biologi Universitas Andalas Psikis : Jurnal Psikologi Islami Bioeksperimen: Jurnal Penelitian Biologi BioCONCETTA Jurnal Counsellia JURNAL PENGABDIAN KEPADA MASYARAKAT Jurnal Biologi Tropis Jurnal Hilirisasi IPTEKS (JHI) JURNAL ILMU PENDIDIKAN AHLUSSUNNAH Jurnal Pengabdian kepada Masyarakat Nusantara Andalasian International Journal of Agricultural and Natural Sciences
Nofrita Nofrita
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Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Public Health Social Sciences Veterinary Other

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DNA Barcoding and eDNA Metabarcoding for Identification Species: A Case Study (West Sumatra): DNA barcoding and eDNA metabarcoding Roesma, Dewi Imelda; Tjong, Djong Hon; Syaifullah, Syaifullah; Nofrita, Nofrita; Janra, Muhammad Nazri; Aidil, Dyta Rabbani; Prawira, Furqan Dwiki Lintang; Salis, Viola Mutiara
Journal of Tropical Life Science Vol. 15 No. 1 (2025)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/

Abstract

The biodiversity of freshwater fish is important to study because there is data and information that remain undiscovered. The waters of Sumatra, especially West Sumatra, are areas with high freshwater fish diversity but have limited information. Providing information and genetic data has become one of the important things to conduct. DNA barcoding and eDNA metabarcoding have become molecular methods for identifying species and providing information about the presence of species in a region. A study using DNA barcoding and eDNA metabarcoding was conducted on freshwater fish in several locations in West Sumatra. Isolation and amplification of DNA were performed directly on individual samples and sequenced using conventional methods (Sanger sequencing) to generate DNA barcodes. Water samples were collected (2 liters) at each location using a sterile bottle. The water samples were filtrated, isolated, and amplified using universal primer and sequenced with next-generation sequencing techniques. The study successfully collected 25 species belonging to 14 genera, 2 families, and 1 order. A total of 134 sequences from West Sumatra with a length of 648-670 bp were analyzed. All DNA barcodes were submitted to the BOLD System and GenBank, NCBI. The mean Kimura two-parameter model (K2P) genetic distances within species, genera, families, and orders were 0.7%, 8.3%, 15.8%, and 21.3%, respectively. The eDNA metabarcoding technique has successfully detected three native fish species in the waters of West Sumatra (Barbonymus schwanefeldii, Mystacoleucus padangensis, and Rasbora jacobsoni). The availability of fish DNA barcodes in reference databases is crucial for the success of identification using eDNA metabarcoding. Combining identification using conventional methods and eDNA metabarcoding can provide more reliable results and become a reference for future freshwater monitoring.
Food Composition of Frigate Tuna (Auxis thazard Lacepède,1800) Caught by Fisherman in West Sumatra Waters Saniyyah, Jauharah; Nofrita, Nofrita; Nurdin, Jabang
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8002

Abstract

Frigate tuna (Auxis thazard) is a pelagic and neritic fish resource that has economic value. Fish diet is one of the important information in monitoring the sustainability of fisheries resources in the future. This study aims to determine the composition of food and determine the Index of Preponderance of frigate tuna caught by fishermen in West Sumatra waters. The method used in this study was random sampling by taking 98 samples of frigate tuna obtained from the catch of fishermen in West Sumatra waters landed at the Air Bangis Fish Landing Center, West Pasaman Regency, and Gaung Fish Auction Site, Padang. The analysis used the Index of Preponderance to determine the main food, additional food, and complementary food. The results showed the composition of food in the stomach of frigate tuna consisted of 3 groups of organisms, namely zooplankton (61.04%), phytoplankton (31.82%), and pisces (7.13%). The main food of frigate tuna is zooplankton (62.10%), while the additional food are phytoplankton (27.73%), and pisces (10.16%). The availability of frigate tuna food in West Sumatra waters supports the sustainability of the frigate tuna population in these waters.
Reproductive Biology of Frigate Tuna (Auxis thazard Lacepède, 1800) Caught by Fishermen in West Sumatra Waters Nofrita, Nofrita; Nurdin, Jabang; Bintari, Andhini Nur
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8004

Abstract

The frigate tuna (Auxis thazard) is important to the fishing industry in West Sumatra. The increasing demand for frigate tuna causes this fish to continue to be exploited. Exploitation carried out continuously can reduce frigate tuna production, so research needs to be done. The research was conducted to: (1) Know the growth pattern and factor condition of frigate tuna (A. thazard) in West Sumatra waters; (2) Know the reproductive biology of frigate tuna (A. thazard). Samples were collected from fishermen's catch in the waters of West Sumatra. Random sampling was conducted, and samples were measured for length and weight, followed by dissection to ascertain sex ratio, gonadal maturity level, gonadal maturity index, and fecundity. The results indicated that the fish's length ranged from 350 to 475 mm, with an average of 401.48 mm, and their weight ranged from 408 to 1,339 grams, with an average of 755.8 grams. The growth pattern is identified as positive allometric (b>3), indicating that weight gain surpasses length increases. The condition factor is in stable condition with an average of 1.24. The sex ratio is in an unbalanced condition with a ratio of males and females of 0.9: 1. The level of gonad maturity obtained is GML III and GML IV. The gonad maturity index of male fish ranged from 1.71 to 3.04 and female fish from 1.28 to 2.50. Estimated fecundity ranged from 261,468 to 2,775,590 grains. Based on several factors included in this study, the reproductive biology of frigate tuna in the waters of West Sumatra is favorable.
Co-Authors . Mansyurdin A.A. Ketut Agung Cahyawan W Abd. Rasyid Syamsuri Afrizal Sano Ahmad Yazid Aidil, Dyta Rabbani Alfaiz Alfaiz Asful, Ferdhinal Ashrifurrahman, Ashrifurrahman Asmaiwaty Arief Aszareta, Muhammad Andoni Aziz, M. Abdul Bayu Marsha. Ds Bintari, Andhini Nur Bramadi Arya BUSTANUL ARIFIN DAFIT SATRIA Dapit Pernalis Deden Tri Sang Putra Destaria Sudirman Nurhaty, Destaria Sudirman Dewi Imelda Roesma Djong Hon Tjong Dosi Juliawati EFATRICIA NIATI Efrizal Efrizal Eka Sefputri Elmayyeti Elmayyeti Elvi Susanti Eti Yerizel Fadillah Fadillah Fadillah Fadjar Goembira Faiz, Alfaiz Fani Karmila Fitri Yumilda Fitria Kasih Frenadin Adegustara Gustia Manasari Hamida Hamida Hasneli, Hasneli Hengki Yandri Henny Herwina Indra Ibrahim Irvan Apandi Ismi Auldra Efendi IYAS IYAS Izmiarti Izmiarti, Izmiarti Jabang Nurdin Janra, Muhammad Nazri Lasma Farida Luzi Ratnasari M. Haris Maliza, Rita Marisa Nanda Marwisni Hasan Melda Krisna Dasvita Mellyarti Mellyarti Mellyarti Syarif Mhd Nur Allatif Muhamma Idris Muhammad Afif Muhammad Hamdi Ibrahim Muhammad Idris Muhammad Idris Muhammad Ilham Samudra Muhammad Nazri Janra Muhammad Nazri Janra Murisal Murisal muryani, nova Musda Rukmana Melka Nasution, Risda Afifi Nefri Yanra Niki Yulita Herman Nola Karmila Sari Noliza Noliza Novia Susana Novita Ratna Sari Olly Norita Tetra Pratama Elisa, Tasya Putri Pratama, Raihan Anugrah Prawira, Furqan Dwiki Lintang Puji Yani Pratama Putra Santoso Putri, Eggy Triana Putri, Syntia Mai Randi Saputra Reski Puspita Sari Reziq Marchellino Irwan Ridho, Muhammad Syifa'ur Rila Rahma Mulyani Rini Septiani Yunas Robby Jannatan Ryan Hidayat Rafiola Salis, Viola Mutiara Saniyyah, Jauharah Sari Murni Sari, Azmatul Khairiyah Silva Lestari Sinurat, Arthauly Yopita Syaifullah Syaifullah Tasya Putri Pratama Elisa Taufiq Ihsan Tofrizal Tofrizal, Alimuddin Wardahtul Aini Wilson Novarino Winda Rahayu Yasir Muharram Fauzi Yelvi Monasari Yulinar Yulinar Yuzarion, Yuzarion Zaini Zaini