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All Journal Biotika: Jurnal Ilmiah Biologi Jurnal Ilmu Dasar AL KAUNIYAH Jurnal Florea Pro-Life Edumatsains Padjadjaran Journal of Dentistry Jurnal Biodjati Jurnal Cendekia : Jurnal Pendidikan Matematika KAIBON ABHINAYA : JURNAL PENGABDIAN MASYARAKAT Sains dan Matematika Jurnal Agrinika : Jurnal Agroteknologi dan Agribisnis Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Jurnal Riset dan Inovasi Pendidikan Sains (JRIPS)

Mohamad Nurzaman

Departemen Biologi Fakultas Matematika Dan Ilmu Pengetahuan Alam Universitas Padjadjaran

Author-ID : 336122

Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Control & Systems Engineering Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Other

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PENGARUH POLYETHYLENE GLYCOL (PEG) TERHADAP KADAR KUERSETIN KULTUR KALUS Chrysanthemum morifolium Ramat PADA KONDISI PENCAHAYAAN BERBEDA Tia Setiawati; Syifa Fauzia Zazuli; Annisa Annisa; Mohamad Nurzaman; Budi Irawan
Al-Kauniyah: Jurnal Biologi Vol 13, No 1 (2020): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

AbstrakKrisan (Chrysanthemum morifolium Ramat) mengandung senyawa kuersetin dengan efek farmakologi yang sangat luas. Penambahan polyethilene glycol (PEG) dapat dimanfaatkan untuk meningkatkan produksi metabolit sekunder secara in vitro. Penelitian ini bertujuan untuk mengetahui pengaruh PEG terhadap pertumbuhan kalus krisan dan kadar kuersetin 3-O-rhamnosida pada pencahayaan yang berbeda. Penelitian menggunakan Rancangan Acak Lengkap (RAL). Eksplan kalus berumur 45 hari setelah tanam (HST) disubkultur pada media MS + 4 ppm 2,4-D dengan penambahan PEG dalam lima taraf konsentrasi yaitu 0, 10, 20, 30, dan 40 ppm. Kultur diinkubasi pada kondisi gelap dan terang. Parameter yang diamati adalah warna, tekstur, ukuran, berat basah, dan berat kering kalus serta kadar kuersetin 3-O-rhamnosida. Data kuantitatif dianalisis menggunakan Analisis Varians dan Uji Jarak Berganda Duncan (α 5%), sedangkan data kualitatif dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa kalus pada kondisi terang berwarna cokelat dan cokelat kehijauan, sedangkan pada kondisi gelap berwarna putih kecokelatan. Perlakuan 10 ppm PEG menghasilkan berat basah dan berat kering kalus tertinggi baik pada kondisi terang maupun gelap berturut-turut 1,97 g dan 2,92 g; 0,94 g dan 1,09 g. Kadar kuersetin 3-O-rhamnosida tertinggi pada kondisi gelap dan terang terdapat pada perlakuan 10 ppm PEG berturut-turut 1,72 µg/g berat kering (BK) dan 2,59 µg/g BK.Abstract Chrysanthemum morifolium Ramat. contains quercetin with very broad pharmacological effects. The addition of PEG can be used to increase the production of secondary metabolites using in vitro method. This study aimed to determine the effect of PEG on the growth of Chrysanthemum callus and quercetin 3-O-rhamnoside content in different lighting conditions. The experimental design used was a Completely Randomized Design. After 45 days, callus were subcultured on MS medium + 4 ppm 2,4-D which PEG was added in five concentration levels (0, 10, 20, 30, and 40 ppm). Culture was incubated in dark and light conditions. Parameters observed were color, texture, size, wet weight and dry weight of callus, also quercetin 3-O-rhamnoside levels. Quantitative data was analyzed using Analysis of Variance and Duncan's Multiple Distance (α 5%). Qualitative data was analyzed descriptively. The results showed that in light condition, the callus has brown and greenish brown color, whereas in dark, it has brownish white color. The 10 ppm PEG treatment produced the highest fresh weight and dry weight in both light and dark conditions of 1.97 g and 2.92 g, 0.94 g and 1.09 g, respectively.The highest quercetin 3-O-rhamnoside content in dark and light conditions were founded in 10 ppm PEG treatment of 1.72 µg/g dry weight (DW) and 2.59 µg/g DW.

Pemanfaatan Tumbuhan Famili Zingiberaceae oleh Masyarakat Sekitar Kawasan Wisata Pantai Rancabuaya Kecamatan Caringin Kabupaten Garut Asep Zainal Mutaqin; Mohamad Nurzaman; Tia Setiawati; Ruly Budiono; Ela Noviani
Sains dan Matematika Vol. 5 No. 2 (2017): April, Sains & Matematika
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Indonesia adalah negara yang memiliki keragaman suku bangsa dan budaya, termasuk pengetahuan tradisional di dalamnya. Salah satu pengetahuan yang ada di masyarakat adalah pengetahuan mengenai pemanfaatan tumbuhan. Penelitian ini dilakukan untuk mendokumentasikan pemanfaatan jenis-jenis tumbuhan famili Zingiberaceae oleh masyarakat desa di kawasan Wisata Pantai Rancabuaya Kecamatan Caringin Kabupaten Garut. Metode yang digunakan dalam penelitian ini adalah metode kualitatif bersifat deskriptif analisis. Teknik pengumpulan data dilakukan dengan cara observasi langsung dan wawancara semistruktur terhadap informan kunci. Penentuan informan dilakukan dengan teknik snowball sampling. Hasil wawancara menunjukan bahwa terdapat 12 jenis tumbuhan famili Zingiberaceae yang dimanfaatkan masyarakat, yaitu laja bodas (Alpinia galanga (L.) Willd.), laja beureum (Alpinia purpurata K. Schum.), kapolaga (Amomum cardamomum Maton), koneng temen (Curcuma domestica Val.), koneng gede (Curcuma xanthorrhiza Roxb.), cikur (Kaempferia galanga Linn.), jahe gajah (Zingiber officinale var. Roscoe), jahe emprit (Zingiber officinale var. Amarum), jahe beureum (Zingiber officinale var. Rubrum), koneng bodas (Curcuma zedoaria (Berg.) Rosc.), lempuyang wangi (Zingiber aromaticum Val.), dan panglay (Zingiber cassumunar Roxb.). Berdasarkan genusnya, masyarakat memanfaatkan Alpinia, Amomum, dan Kaempferia sebagai bumbu masak dan obat; Curcuma sebagai bumbu masak, bahan jamu, dan obat; serta Zingiber sebagai obat, bumbu masak, bahan minuman, dan ritual adat. Tumbuhan-tumbuhan tersebut merupakan tumbuhan liar dan hasil budidaya yang diperoleh dari pekarangan, kebun, dan sawah. Indonesia is a country that has a diversity of ethnic groups and cultures, including traditional knowledge in it. One of the existing knowledge in society is knowledge about the use of plants. This research was conducted to document the utilization of Zingiberaceae family of plant species by rural communities in the Rancabuaya Coastal area of Caringin District, Garut Regency. The method used in this research is descriptive qualitative analysis. Data collection techniques were carried out by direct observation and semistructured interviews of key informants. Determination of informants is done by snowball sampling technique. Interview results show that there are 12 species of plants of the Zingiberaceae family that are utilized by the community, namely laja bodas (Alpinia galanga (L.) Willd.), laja beureum (Alpinia purpurata K. Schum.), kapolaga (Amomum cardamomum Maton), koneng temen (Curcuma domestica Val.), koneng gede (Curcuma xanthorrhiza Roxb.), cikur (Kaempferia galanga Linn.), jahe gajah (Zingiber officinale var. Roscoe), jahe emprit (Zingiber officinale var. Amarum), jahe beureum (Zingiber officinale var. Rubrum), koneng bodas (Curcuma zedoaria (Berg.) Rosc.), lempuyang wangi (Zingiber aromaticum Val.), dan panglay (Zingiber cassumunar Roxb.). Based on its genus, people use Alpinia, Amomum, and Kaempferia as cooking spices and medicines; Curcuma as cooking spices, herbal ingredients, and medicine; and Zingiber as medicine, cooking spices, beverage ingredients, and traditional rituals. These plants are wild plants and cultivation results obtained from the yard, garden, and rice fields.

Empowering the Rural Communities through Education on the Use of Chrysanthemums as Medicinal Plants and Herbal Tea Tia Setiawati; Annisa Annisa; Mohamad Nurzaman; Rusdi Hasan; Nining Ratningsih
KAIBON ABHINAYA : JURNAL PENGABDIAN MASYARAKAT Vol. 5 No. 1 (2023)
Publisher : Universitas Serang Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30656/ka.v5i1.5347

Chrysanthemum is widely known as ornamental plants, but chrysanthemums can also be used as medicinal plants and can be processed into healthy beverages known as chrysanthemum tea. The purpose of the PPM activity is to educate the community, especially PKK women who are participants in this activity about the role of chrysanthemum plants in the prevention and treatment of various diseases such as fever, hypertension, osteoporosis, eye healthy and making chrysanthemum tea as a healthy beverage. PPM activities was carried out through outreach activities with presentations and discussions (question and answer), demonstrations of making chrysanthemum tea, and distributing questionnaires to determine participants understanding before and after the implementation of PPM activities. The results of the questionnaire showed that participants knowledge about the use of chrysanthemum as medicinal ingredients increased after participating in outreach activities, as well as the interest in chrysanthemum tea cultivation and entrepreneurship.

Desain Kurikulum dan Konversi Hasil Kegiatan MBKM Program Studi Sarjana Matematika Edi Kurniadi; Mohamad Nurzaman; Nurul Gusriani
Jurnal Cendekia : Jurnal Pendidikan Matematika Vol 7 No 2 (2023): Jurnal Cendekia: Jurnal Pendidikan Matematika Volume 7 Nomor 2 Tahun 2023
Publisher : Mathematics Education Study Program

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/cendekia.v7i2.2364

Program Merdeka Belajar Kampus Merdeka (MBKM) adalah suatu program untuk meningkatkan kompetensi tambahan mahasiswa atau capaian pembelajaran lulusan di luar program studinya. Kegiatan MBKM yang dimaksud adalah delapan bentuk kegiatan pembelajaran yaitu magang, asistensi mengajar, pertukaran pelajar, studi independent, penelitian, kewirausahaan, proyek kemanusian, dan membangun desa atau Kuliah Kerja Nyata (KKN) tematik. Metode penelitian yang digunakan adalah metode penelitian kualitatif khususnya metode studi kasus phenomenological research. Tujuan penelitian ini adalah untuk mendesain bagaimana cara mengonversi hasil kegiatan MBKM tersebut terkait kedudukannya, khususnya dalam kurikulum program studi sarjana matematika. Metode konversi yang diperoleh dapat dipergunakan tidak hanya di Program Studi Matematika tetapi untuk program studi-program studi lainnya yang menjalankan program MBKM bagi mahasiswanya. Tahapan pelaksanaan MBKM sendiri terdiri dari tiga tahapan yaitu pertama pendaftaran baik di tingkat program studi, mitra di laman kampus merdeka, ke dua monitoring dan evaluasi pelaksanaan MBKM seperti logbook laporan dan laporan kegiatan, dan yang ke tiga penilaian berupa presentasi dan laporan akhir. Setiap tahapan dibuat rubrik penilaian sebagai bahan untuk konversi hasil akhir kegiatan MBKM. Hasil utama yang diperoleh dari penelitian ini berupa teknik baku dalam cara menilai dan mengonversi hasil MBKM.

Aplikasi Asam Humat dan Pupuk Organik Cair Ekstrak Rumput Laut untuk Meningkatkan Pertumbuhan dan Kandungan Flavonoid Brassica juncea L. Setiawati, Tia; Fitria, Sania Septiani; Nurzaman, Mohamad; Hasan, Rusdi; Mutaqin, Asep Zainal; Lestari, Ani
Jurnal Pro-Life Vol. 11 No. 2 (2024): Juli
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/jpvol6Iss2pp102

This research was conducted with the aim of obtaining the optimal dose of humic acid (HA) and liquid organic fertilizer (LOF) from seaweed extract that can enhance the growth and total flavonoid content of Brassica juncea L. The method used in this study was experimental with a randomized complete block design with two factors. The first factor was HA, consisting of 4 dose levels: 0, 4, 8, and 12 g/kg, while the second factor was LOF from seaweed extract, consisting of 4 dose levels: 0, 1, 2, and 3 mL/L. Each treatment was replicated four times. Parameters were observed on the 42nd day, including plant height, leaf number, leaf area, dry weight, and total flavonoids. Data were analyzed using Analysis of Variance (ANOVA) and followed by Duncan's Multiple Range Test with a confidence level of 95%. The results showed that the dose of 4 g/kg HA and 2 mL/L LOF was the best dose for enhancing growth and total flavonoid content, producing an average plant height of 19.25 cm, a leaf number of 5.75, a total leaf area of 179.8 cm2, a dry weight of 0.57 grams, and a total flavonoid content of 0.106 mg QE/g sample.

Analysis of Secondary Metabolites of Shoot, Callus Culture and Field Plant of Chrysanthemum morifolium Ramat Setiawati, Tia; Ayalla, Alma; Nurzaman, Mohamad; Kusumaningtyas, Valentina A.; Bari, Ichsan
Jurnal ILMU DASAR Vol 21 No 1 (2020)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

The chrysanthemum plant (Chrysanthemum morifolium Ramat.) contains many secondary metabolites such as flavonoids and various volatile compounds that can be utilized as drugs. Tissue culture can be an alternative to enhance the production of certain secondary metabolite. The study aimed to determine the types of secondary metabolites that contained in shoot culture, callus and field plants of C. morifolium. The research method was exploration in the laboratory to analyze and compare the content of secondary metabolite from shoot culture, callus and field plants of C. morifolium. Callus was induced by explants of C. morifolium plantlet stems and leaves respectively on MS medium with an addition of 3 ppm 2,4-D + 2 ppm kinetin and 4 ppm 2,4-D. For shoot culture, single nodule explants with one leaf were planted on MS media with the addition of 1 ppm BAP. The secondary metabolite compouds were analized and identified by GC-MS (Gas Chromatography-Mass Spectrometry). The results showed that various types of secondary metabolites contained in shoot culture, callus and field plants of C. morifolium. In callus culture from leaf explants, four compounds from groups of alcohol, acetic acid and organosilicon were identified, whereas in callus culture from stem explants were identified eight compounds from aldehydes, esters, alkanes, and carboxylic acids group. In the shoot culture, nine compounds of alcohol, ketone, aldehyde, cycloalkane and organosilicon group were identified, while in the field plants five compounds were identified from the cycloalkanes, ketones, organoborones and organosilicon group. Some detected compounds have a potential as precursors of alkaloid, phenolic, and flavonoid.Keywords: chrysanthemum, culture, shoots, callus, secondary metabolites.

Morphoanatomy and Phytochemical Content of Sente Leaves (Alocasia macrorrhizos (L.)G.Don) under Different Light Intensity in Arboretum Universitas Padjadjaran Rachma, Nadhira Zaachrany; Mutaqin, Asep Zainal; Setiawati, Tia; Nurzaman, Mohamad; Hasan, Rusdi
Florea : Jurnal Biologi dan Pembelajarannya Vol. 11 No. 1 (2024)
Publisher : UNIVERSITAS PGRI MADIUN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25273/florea.v11i1.21146

Light intensity in plants influences their growth potential as well as their morphological, anatomical, and physiological structures. This study aimed to observe the leaf area, leaf thickness, leaf type, stomatal type, stomatal density, chlorophyll content, and phytochemical content of Sente leaves (Alocasia macrorrhizos) in two locations with different light intensities. This exploratory research used a survey method to determine the research location, and environmental parameter measurements was used as supporting data. Morphological and anatomical parameters and chlorophyll content were assessed using a quantitative approach, while phytochemical content was determined qualitatively and analyzed descriptively. The results showed differences in the morphology, anatomy, chlorophyll content, and phytochemicals of Sente leaves in varying light intensities. Morphologically, the leaves of Sente plants in shaded locations had larger surface areas, were thinner, and appeared greener compared to those in unshaded locations. The stomatal density of leaves in unshaded areas was higher (76.43 cells/mm ²) compared to shaded areas (56.05 cells/mm ²). The chlorophyll content in shaded locations was higher (82.03 CCl) than in unshaded locations (41.7 CCl). Phytochemical tests for flavonoids and saponins showed higher compound levels in leaves from unshaded locations, while tannin tests revealed higher levels in shaded locations compared to unshaded ones. Meanwhile, tests for alkaloids and quinones yielded negative results.

Characteristics of Stomata and Leaf Thickness in Several Liliales Plants Hasan, Rusdi; Nurzaman, Mohamad; Setiawati, Tia; Mutaqin, Asep Zainal
Florea : Jurnal Biologi dan Pembelajarannya Vol. 11 No. 2 (2024)
Publisher : UNIVERSITAS PGRI MADIUN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25273/florea.v11i2.21297

This study examines the stomatal characteristics and leaf thickness of six species within the Liliales: Gloriosa superba, Sansevieria trifasciata, Aloe vera, Cordyline terminalis, Pleomele angustifolia, and Allium fistulosum. Stomatal density, type, and distribution were measured to understand their relationship with leaf morphology. The results show that Gloriosa superba has a stomatal density of 148.72/mm² and an index of 0.295, with thin leaves. Sansevieria trifasciata, with its thick, fleshy leaves, has a lower stomatal density of 15.39/mm² and an index of 0.031. Aloe vera, another species with thick leaves, has a stomatal density of 38.47/mm² and an index of 0.067. In contrast, Cordyline terminalis and *Pleomele angustifolia, which have thin leaves, exhibit higher stomatal densities of 192.31/mm² and 128.21/mm², respectively. Their stomatal indices are 0.092 for Cordyline terminalis and 0.163 for Pleomele angustifolia. Allium fistulosum also has thin leaves, with a stomatal density of 100/mm² and a high stomatal index of 0.390. The study reveals that species with thicker leaves tend to have lower stomatal densities, a feature that helps reduce water loss in arid environments. These findings contribute to a deeper understanding of plant adaptation mechanisms and have implications for improving water-use efficiency in agriculture and conservation efforts.

The Growth of Oyster Mushroom on Ramie Chip Waste-Derived Media was Accelerated by Rice-washed Water Budiono, Ruly; Washfanisa, Handina Alya; Mutaqin, Asep Zainal; Kusmoro, Joko; Nurzaman, Mohamad; Setiawati, Tia; Hasan, Rusdi
Jurnal Biodjati Vol 9, No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.33433

Rice-washed water is wastewater resulted from the rice cleaning process that is rich in nutrients. The use of ramie chip waste can be considered a potential substrate due to its lignocellulosic nature, similar to other wastes studied. This study aimed to examine the effect of rice-washed water on the mycelium and pinhead growth of white oyster mushrooms grown in the ramie chip waste as the growing medium. The experiment was conducted for three months using a completely randomized design with four rice-washed water treatments and six replications. The rice-washed water concentration treatments were 0% (K0), 25% (K1), 50% (K2), and 100% (K3), which were added to the ramie chip waste as the growing medium for white oyster mushroom. The time of mycelium propagation velocity, the time the first pinhead appearance, the number of mushroom bodies, and the largest cap diameter were observed. The results showed that ramie chip waste-derived media was suitable to cultivate white oyster mushrooms. Adding 100% rice-washed water to the media significantly affected the speed of mycelium propagation (16.51 days) compared to control (30 days) and the appearance of the first pinhead (31.5 days) compared to control (38.5 days) as well as the number of fruiting bodies of mushroom (8.8) compared to control (6.2). However, the rice-washed water application did not significantly affect the diameter of the largest mushroom cap.

Karakteristik Morfo-Anatomi serta Kandungan Klorofil dan Kandungan Fitokimia Tanaman Ketul (Bidens pilosa) pada Tempat dengan Intensitas Cahaya yang Berbeda di Arboretum Universitas Padjadjaran Marcelline, Karen; Nurzaman, Mohamad; Setiawati, Tia
Pro-Life Vol. 12 No. 1 (2025): Maret
Publisher : Program Studi Pendidikan Biologi Fakultas Keguruan dan Ilmu Pendidikan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Bidens pilosa is a plant known for its secondary metabolites with therapeutic properties. This study aimed to examine the effects of different light intensities on the morphology, anatomy, chlorophyll content, and secondary metabolite production of B. pilosa in the Arboretum of Universitas Padjadjaran. The shaded and unshaded area had light intensities of 5828 and 32768 lux, respectively. Leaf samples were collected from three different individuals in each area, with three leaves taken per plant. Morphological and anatomical traits such as leaf thickness, leaf area, stomatal density, and chlorophyll content were observed, along with secondary metabolite content. Results showed that plants in the shaded area had thinner leaves (0,19 mm) and lower stomatal density (275,16 cells/mm2) but a larger leaf area (4 cm2) and higher chlorophyll content (29,33 CCI) compared to leaves in the unshaded area, which had thicker leaves (0,213 mm), higher stomatal density (310,83 cells/mm2), a smaller leaf area (2,33 cm2), and lower chlorophyll content (27,27 CCI). Alkaloids, flavonoids, tannins, and saponins were detected in both conditions, with a higher level of alkaloid and tannin in unshaded plants. These findings enhance our understanding of the relationship between light intensity influences plant morphology, photosynthesis, and secondary metabolite production, with potential applications in cultivation and medicinal use.

Co-Authors Angger Waspodo Anggita Levi Astuti Ani Lestari Annisa Annisa Annisa Annisa Annisa Annisa Annisa Nur Arofah Arianti, Tanti Asep Zainal Mutaqin Asri Peni Wulandari Audia Putri Kamajaya Aulia Nurmalasari Pane Nurmalasari Pane Auliya Zahra Ayalla, Alma Azifah An’amillah Bari, Ichsan Budi Irawan Budi Irawan Dikdik Kurnia Edi Kurniadi Ela Noviani Elizabeth Fitriana Sari Euis Julaeha Fetriyuna Fetriyuna Fikriyah, Nanda adzka Fitria, Sania Septiani Irma Wahyuningsih Johan Iskandar Joko Kusmoro Julianty Nur C. Lidwina E. Septiany Marcelline, Karen Maryam Alifah Maulidiyah Maulidiyah Meilinda, Pudji Michelle Azista N. Casandra Nining Ratningsih Noviani, Ela Noviyanti Soleha Nurul Gusriani Oman Karmana Permadi, Nandang Rachma, Nadhira Zaachrany Rully Budiono Ruly Budiono Rusdi Hasan Rusdi Hasan Sephianti, Selvi Sri Ratu Dewi Pridani Sugarti, Dini Syifa Fauzia Zazuli Tarwinih Tarwinih Tati Herlina Tentani Buhti Amadea Tia Setiawati Titin Supriatun Titin Supriatun Sadeli Tri Mayanti Valentina Adimurti Kusumaningtyas Washfanisa, Handina Alya Yudi Rosandi

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