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KANDUNGAN FITOKIMIA DAUN TEMBELEKAN (Lantana camara L) PADA DUA TEMPAT DENGAN INTENSITAS CAHAYA BERBEDA Fikriyah, Nanda adzka; Tia Setiawati; Rusdi Hasan; Mohamad Nurzaman; Asep Zainal Mutaqin
Jurnal Riset dan Inovasi Pendidikan Sains (JRIPS) Vol. 4 No. 1 (2025): Januari
Publisher : Universitas Muhammadiyah Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36085/jrips.v4i1.7955

Abstract

Tembelekan (Lantana camara L) adalah salah satu tumbuhan yang banyak dimanfaatkan untuk pengobatan. Tumbuhan ini mengandung banyak senyawa fitokimia yang bermanfaat untuk kesehatan seperti alkaloid, flavonoid, saponin dan lainnya. Salah satu faktor yang memengaruhi kandungan fitokimia tanaman adalah intensitas cahaya karena dapat memengaruhi proses metabolisme tumbuhan hingga pembentukan senyawa metabolit sekunder. Oleh karena itu, dilakukan penelitian untuk menguji kandungan fitokimia yang terdapat di daun tembelekan (Lantana camara L) pada lokasi dengan intensitas cahaya yang berbeda yaitu sampel dari daerah yang gelap atau ternaungi dan sampel dari daerah yang terang atau terkena sinar matahari langsung. Penelitian ini dilakukan dengan metode survei dan uji kualitatif dengan pendekatan deskriptif. Hasil penelitian menunjukkan perbedaan intensitas cahaya pada lokasi yang terang sebesar 9925 lux sedangkan daerah gelap sebesar 1500 lux. Ditemukan senyawa fitokimia tembelekan (Lantana camara L) pada sampel terang yaitu senyawa fenol, saponin dan flavonoid sedangkan pada sampel di daerah yang ternaungi atau gelap ditemukan senyawa fenol, flavonoid dan kuinon. Kata Kunci : Fitokimia, Intensitas Cahaya, Lantana camara, Uji Fitokimia
The antibacterial effect of β-pinene derived from Citrus aurantifolia peel against oral Streptococcus mutans ATCC 25175 Julaeha, Euis; Herlina, Tati; Nurzaman, Mohamad; Mayanti, Tri; Kurnia, Dikdik; Sari, Elizabeth Fitriana
Padjadjaran Journal of Dentistry Vol 33, No 1 (2021): March 2021
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol33no1.29200

Abstract

Introduction: Streptococcus mutans has been known to play a major role in dental caries development. This tooth decay is the most common oral disease affecting people in the world. Hence, discovering the new herbal antibacterial agent seems to become more promising yet challenging. One of natural antibacterial source is the peel of Citrus aurantifolia, as it may contain antibacterial active compounds against pathogenic oral microbes. This study was aimed to isolate antibacterial compound from essential oil of Citrus aurantifolia and to analyse its antibacterial activity against oral Streptococcus mutans ATCC 25175. Methods: Type of research was laboratory experimental. The essential oil was isolated from peel of Citrus aurantifolia by hydro-distillation technique and further isolation of antibacterial compounds was conducted by combination column chromatography using organic solvent, and the structure was determined by UV-Vis, infrared (IR), 1D NMR of 1H-, 13C-NMR and DEPT 135° in CDCl3 spectrometers methods. The antibacterial activity was tested against Streptococcus mutans ATCC 25175 using the Kirby-Bauer method. Results: 0.75% yield was obtained from the essential oil of peel Citrus aurantifolia and after purification, an antibacterial compound was identified as β-pinene with the molecular formula C10H16. Furthermore, the β-pinene from peel of Citrus aurantifolia exerted inhibition growth against Streptococcus mutans at concentration of 2000, 1000, and 500 ppm by showing respectively, inhibition values of 13.0, 11.9, and 11.6 mm on paper disk. Conclusion: In the present study, the essential oil of Citrus aurantifolia peel is proven to contain prospective antibacterial compound (β-pinene) that potentially can be used as natural antibacterial alternative choice to manage oral disease associated with Streptococcus mutans infection.
The Growth of Oyster Mushroom on Ramie Chip Waste-Derived Media was Accelerated by Rice-washed Water Budiono, Ruly; Washfanisa, Handina Alya; Mutaqin, Asep Zainal; Kusmoro, Joko; Nurzaman, Mohamad; Setiawati, Tia; Hasan, Rusdi
Jurnal Biodjati Vol 9 No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.33433

Abstract

Rice-washed water is wastewater resulted from the rice cleaning process that is rich in nutrients. The use of ramie chip waste can be considered a potential substrate due to its lignocellulosic nature, similar to other wastes studied. This study aimed to examine the effect of rice-washed water on the mycelium and pinhead growth of white oyster mushrooms grown in the ramie chip waste as the growing medium. The experiment was conducted for three months using a completely randomized design with four rice-washed water treatments and six replications. The rice-washed water concentration treatments were 0% (K0), 25% (K1), 50% (K2), and 100% (K3), which were added to the ramie chip waste as the growing medium for white oyster mushroom. The time of mycelium propagation velocity, the time the first pinhead appearance, the number of mushroom bodies, and the largest cap diameter were observed. The results showed that ramie chip waste-derived media was suitable to cultivate white oyster mushrooms. Adding 100% rice-washed water to the media significantly affected the speed of mycelium propagation (16.51 days) compared to control (30 days) and the appearance of the first pinhead (31.5 days) compared to control (38.5 days) as well as the number of fruiting bodies of mushroom (8.8) compared to control (6.2). However, the rice-washed water application did not significantly affect the diameter of the largest mushroom cap.
Kerapatan Stomata dan Kadar Klorofil Tumbuhan Clausena Excavata berdasarkan Perbedaan Intensitas Cahaya Budiono, Ruly; Sugarti, Dini; Nurzaman, Mohamad; Setiawati, Tia; Supriatun, Titin; Mutaqin, Asep Zainal
Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) 2016: Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (748.752 KB)

Abstract

Penelitian ini dilakukan untuk mengetahui kerapatan stomata dan kadar klorofil tumbuhan Clausena excavata atau ki baceta berdasarkan intensitas cahaya di Cagar Alam Bojonglarang Jayanti Kabupaten Cianjur. Teknik pengambilan sampel menggunakan metode survey dan untuk analisis sampel menggunakan spektrofotometer. Pengambilan sampel daun dengan intensitas cahaya 0-500 lux, 500-1000 lux dan 1000-1500 lux dilakukan dengan tiga kali pengulangan. Sampel daun diambil sebanyak 6 helai kemudian dilakukan pengamatan kerapatan stomata dan ekstraksi untuk pengukuran kadar klorofil. Hasil penelitian menunjukan bahwa kerapatan stomata pada intensitas cahaya 0-500 lux sebesar 149,4/mm², pada intensitas cahaya 500-1000 lux sebesar 156,5/mm², dan pada intensitas cahaya 1000-1500 lux sebesar 166,5/mm². Dari hasil pengukuran kadar klorofil didapatkan bahwa total kadar klorofil pada intensitas 0-500 lux adalah sebesar 25,44 mg/L, pada intensitas 500-1000 lux sebesar 47,70 mg/L, dan pada intensitas 1000-1500 lux sebesar 52,45 mg/L
Kadar Klorofil pada Beberapa Tumbuhan Obat di Taman Hutan Raya Ir. H. Djuanda Bandung Nurzaman, Mohamad; Meilinda, Pudji; Budiono, Ruly; Setiawati, Tia; Mutaqin, Asep Zainal
Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) 2016: Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (848.257 KB)

Abstract

Penelitian ini bertujuan untuk mengetahui kadar klorofil dari tumbuhan–tumbuhan obat di Taman Hutan Raya Ir. H. Djuanda Bandung. Teknik pengambilan sampel menggunakan metode survey. Sampel dianalisis menggunakan spektrofotometer. Penelitian dilakukan pada lima titik lokasi. Hasil penelitian menunjukan bahwa ditemukan 15 jenis tumbuhan obat dari 15 famili yang berbeda, yaitu Cananga odorata (Annonaceae), Cinnamomum parthenoxylon (Lauraceae), Cordyline fruticosa (Liliacaee), Datura metel (Solanaceae), Gossampynus heptaphylla (Bombacaceae), Intsia bijuga (Fabaceae), Ixora javinca (Rubiaceae), Lagerstromia speciosa (Lythraceae), Lantana camara (Verbenaceae), Melastoma candidum (Melastomaceae), Pileatrinervia (Urticaceae), Psidium guajava (Myrtaceae), Rhoeo discolor (Commelinaceae), Stercullia Oblongata(Streculiaceae), dan Styrax benzoin (Sytraceae). Jenis yang memiliki kadar klorofil tertinggi adalah Lantana camara dari famili verbenaceae, yaitu sebesar 50,52mg/L. Sedangkan Stercullia Oblongata dari famili Streculiaceae memiliki kadar klorofil terendah dengan nilai sebesar 3,96 mg/L.
KARAKTERISTIK MORFOLOGI, ANATOMI, FITOKIMIA, DAN KLOROFIL DAUN SPESIES FABACEAE DI TWA PANANJUNG, PANGANDARAN Sephianti, Selvi; Mutaqin, Asep Zainal; Budiono, Ruly; Nurzaman, Mohamad; Setiawati, Tia; Hasan, Rusdi
BIOTIKA Jurnal Ilmiah Biologi Vol 23, No 2 (2025): BIOTIKA DESEMBER 2025
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/biotika.v23i2.68018

Abstract

Penelitian ini bertujuan mengkaji karakteristik morfologi, anatomi, kandungan fitokimia, dan klorofil daun tiga spesies famili Fabaceae (Acacia auriculiformis, Dendrolobium umbellatum, dan Cynometra ramiflora) yang tumbuh di Taman Wisata Alam (TWA) Pananjung, Pangandaran. Penelitian ini diharapkan menjadi penelitian dasar tentang potensi spesies Fabaceae dalam bidang farmasi, pertanian, dan konservasi sumber daya alam. Penelitian dilakukan secara observasional melalui pengamatan morfologi dan anatomi daun, pengujian fitokimia secara kualitatif (alkaloid, tanin, dan saponin), serta kadar klorofil daun. Hasil penelitian menunjukkan ada perbedaan karakteristik morfologi, anatomi, dan profil fitokimia antar spesies. A. auriculiformis dan C. ramiflora mengandung alkaloid dan saponin. D. umbellatum mengandung tanin dan saponin. Species yang mengandung klorofil dari yang tertinggi ke terendah secara berurutan adalah C. ramiflora, D. umbellatum, dan A. auriculiformis. Faktor lingkungan yang terukur adalah 859.33-7474 lux untuk intensitas cahaya, 75-83,67% untuk kelembapan udara, 29,23-30,4°C untuk temperatur udara, 6,6-6,93 untuk pH tanah, <2 mg/kg untuk kadar nitrogen tanah, <6,67 mg/kg untuk kadar fosfor tanah, serta <2,34 mg/kg untuk kadar unsur kalium tanah.
Combination of Acclimatization Media for The Primary Hardening Phase of Musa paradisiaca L. var. Kepok Tanjung Plantlets Arianti, Tanti; Hasan, Rusdi; Setiawati, Tia; Nurzaman, Mohamad
Jurnal Agrinika: Jurnal Agroteknologi dan Agribisnis Vol 10 No 1 (2026): MARCH
Publisher : Kadiri University - Faculty of Agriculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30737/agrinika.v10i1.7205

Abstract

Acclimatization is the last and critical stage in the in vitro culture of Musa paradisiaca L. var. kepok tanjung. The primary hardening phase of acclimatization is carried out so that plantlets can adapt from heterotrophic conditions to autotrophic conditions. This study aims to optimize the acclimatization media for M. paradisiaca L. var. kepok tanjung plantlets, an important factor in the acclimatization process. A completely randomized design was applied in this study using 13 different combinations of soil, cocopeat, and charcoal husk media. The observation parameters included morphological parameters (height, number of leaves, leaf area, pseudo-stem diameter, and survival rate) and physiological parameters (wet weight, dry weight, relative water content (RWC), and chlorophyll content index (CCI), which were analyzed using ANOVA or Kruskal-Wallis according to data assumptions. The results showed that all media combinations produced a 100% survival rate, with the best results shown by the cocopeat: charcoal husk (1:1) combination. This media combination provided the highest increase in height (1.05 ± 0.11 cm), pseudo-stem diameter (1.15 ± 0.12 mm), number of leaves (0.75 leaves), leaf area (121.06 ± 20 cm²), fresh weight (8.23 ± 2.99 g), dry weight (0.45 ± 0.16 g), CCI (23.2 ± 3.63), and the highest relative water content (98.47%). The combination of cocopeat and husk charcoal (1:1) was able to maintain moisture and provide good aeration for plantlet growth. Therefore, the combination of cocopeat and charcoal husk (1:1) is recommended as the most effective media for acclimatizing M. paradisiaca L. var. kepok tanjung plantlets in the primary hardening phase.