Claim Missing Document
Check
Articles

Comparative analysis of KnockOut serum replacement and fetal bovine serum for in vitro culture of human dermal fibroblasts Hidayati, Nur Ilham Risma; Kusuma, Indra; Poerwanto, Eko
Universa Medicina Vol. 42 No. 3 (2023)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2023.v42.283-293

Abstract

BACKGROUNDHuman dermal fibroblast (HDF) cultures can be used as a regenerative agent for wound healing. Fetal bovine serum (FBS) as a culture supplement is derived from animals, therefore not being constant in composition, causes variations in research results, thus requiring a substitute such as KnockOut serum replacement (KOSR). This study evaluated the defined KOSR as FBS substitute for HDF culture by measuring the relative expression of basic fibroblast growth factor (bFGF) and keratinocyte growth factor (KGF) messenger RNA (mRNA), HDF cell proliferation, and HDF migration. METHODSHuman dermal fibroblast culture was divided into 2 intervention groups receiving KOSR 5% and KOSR 10%, respectively, and a control group receiving FBS 10%. Reverse transcription polymerase chain reaction (RT-PCR) was used for bFGF and KGF mRNA relative expression at the fifth passage (P5). Cell counting kit-8 (CCK-8) reagent was used for the HDF cell proliferation assay at P5 and the scratch assay was used for HDF cell migration at P6. Data were analyzed using dependent t-test, One-way ANOVA, or Kruskal-Wallis test. RESULTSThere were no significant differences in bFGF and KGF mRNA relative expression and HDF migration velocity between the intervention and control groups (p>0.05 and p>0.05, respectively). The doubling time of the KOSR 5% group showed no significant difference (p>0.05), but KOSR 10% and FBS 10% showed significant differences between treatment days 2-6 and treatment days 6-10 (p<0.05). CONCLUSIONSThe KOSR 10% was comparable to FBS 10% in supporting bFGF and KGF mRNA relative expression, HDF cell proliferation, and HDF cell migration in HDF culture.
ANALISIS PERBANDINGAN KEPUASAN PENUMPANG PADA MASKAPAI PENERBANGAN YANG BEROPERASI DI BANDARA SO’A Dhaka, Nikolaus Meo; Nurdin, Riani; Poerwanto, Eko
Vortex Vol 4, No 1 (2023)
Publisher : Institut Teknologi Dirgantara Adisutjipto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.28989/vortex.v4i1.1422

Abstract

Bandara Udara So’a Bajawa merupakan salah satu bandara udara domestik di pulau Flores kabupaten Ngada, NTT. Terdapat 3 maskapai komersil yang beroperasi di bandara So’a dan tergolong dalam kategori low cost carrier (LCC) yaitu PT. Lion Air, PT. Citilink, dan PT. TransNusa Aviation Mandiri. Bandara Udara So’a bajawa merupakan salah satu bandara udara domestik di pulau Flores kabupaten Ngada, NTT. Terdapat 3 maskapai komersil yang beroperasi di bandara So’a dan tergolong dalam kategori low cost carrier (LCC) yaitu PT. Lion Air, PT. Citilink, dan PT. TransNusa Aviation Mandiri. Kualitas layanan yang optimal dan presepsi harga diharapkan mampu memenuhi harapan penumpang yang menggunakan 3 maskapai yang beroperasi di bandara so’a, untuk itu guna mengetahui kepuasan penumpang penelitian kuantitatif, Data yang digunakan dalam penelitian ini merupakan data primer yang diperoleh melalui pembagian kuesioner kepada penumpang pengguna ketiga maskapai yang beroperasi di bandara So’a. Teknik analisis data yang diterapkan dalam penelitian ini adalah analisis regresi linier berganda menggunakan SPSS versi 16.0 sebagai alat bantu pengolahan data. Hasil penelitian menunjukkan bahwa Lion Air lebih memuaskan dibandingkan dengan Cititlink dan TransNusa.
ANALISIS EFEKTIFITAS DRONE PADA PROSES PEMUPUKAN CAIR UNTUK MENINGKATKAN PRODUKTIVITAS TANAMAN PERTANIAN PADI ORGANIK Hariyanto, Kris; Poerwanto, Eko; Nur Santoso, Prasidananto
Vortex Vol 4, No 2 (2023)
Publisher : Institut Teknologi Dirgantara Adisutjipto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.28989/vortex.v4i2.1658

Abstract

SMK Muhamadiyah Sentolo terletak di wilayah Kabupaten Kulon Progo, Mata pencaharian masyarakat di daerah ini 80% adalah petani yang memiliki etos kerja yang baik..Permasalahan yang dihadapi dalam upaya mengembangkan pertanian antara lain menurunnya minat para generasi muda terhadap usaha di bidang pertanian khususnya tanaman padi, hal tersebut disebabkan karena proses pengerjaan terhadap kegiatan pertanian tersebut relatif cukup melelahkan. Sedangkan generasi tua sudah selayaknya diganti untuk kemudian agar kegiatan pertanian terus berlanjut. Penelitian ini melakukan studi komparasi dengan menggunakan teknologi yang mendukung proses pemupukan cair dengan bantuan drone. Metode yang dilakukan adalah dengan membandingkan penggunaan teknologi drone untuk pemupukan dengan teknologi konvensional atau manual pada tanaman padi selama satu kali musim tanam. Hasil penyemprotan drone yang diperlukan untuk pemupukan pada luasan 1 hektar adalah 0.25 jam, pupuk yang dibutuhkan pada luasan 1 hektar adalah 40 liter, kerja yang dibutuhkan pada luasan 1 hektar adalah 160 liter/jam.
Relationship Between Blood Pressure and Intraocular Pressure in Patients at the Ophthalmology Clinic of Hospital X Jakarta, Indonesia Purwakusuma, Anindya Emiraryanti; Batubara, Lilian; Poerwanto, Eko
International Journal of Integrated Health Sciences Vol 13, No 2 (2025)
Publisher : Faculty of Medicine Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15850/ijihs.v13n2.4319

Abstract

Background: Hypertension, a prevalent and often asymptomatic condition, has been associated with various ocular disorders. Ocular hypertension, in particular, is a notable risk factor for glaucoma. Understanding the relationship between systemic blood pressure and intraocular pressure is important for early detection of ocular hypertension and prevention of vision-threatening complications.Objective: To analyze the relationship between blood pressure and intraocular pressure, as well as the association between hypertension and ocular hypertension. Methods: This observational analytical study used secondary data from the medical records of 74 patients at the ophthalmology clinic of Hospital X Jakarta, collected between January and March 2023. The sample was selected using purposive sampling. Results: Hypertension was observed in 30 individuals (41%), including 19 females (26%) and 11 individuals aged 70–79 years (15%). Ocular hypertension was reported in 17 individuals (23%), with ten (10) females (14%) and nine (9) individuals aged 60–69 years (12%). Chi-Square analysis showed a significant relationship between blood pressure and intraocular pressure, with a p-value of 0.004 (p<α=0.05). In addition, Spearman's test showed correlations between systolic blood pressure and intraocular pressure in both the right eye (r=0.395; p=0.001) and the left eye (r=0.290; p=0.012). Additionally, there were correlations between diastolic blood pressure and intraocular pressure in the right eye (r=0.234; p=0.045) and the left eye (r=0.230; p=0.049).Conclusion: The relationship between blood pressure and intraocular pressure is found to be significant in this study, suggesting that higher blood pressure levels are associated with an increased risk of elevated intraocular pressure.
Pengenalan penggunaan teknologi kedirgantaraan drone untuk meningkatkan mutu siswa SMK Muhammadiyah Sentolo dalam bidang agroindustri Hariyanto, Kris; Poerwanto, Eko
KACANEGARA Jurnal Pengabdian pada Masyarakat Vol 6, No 1 (2023): February
Publisher : Institut Teknologi Dirgantara Adisutjipto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.28989/kacanegara.v6i1.1232

Abstract

Sentolo Muhammadiyah Vocational School is located in the Kulon Progo area, more precisely on Sentolo-Magelang, Ngeplang, Sentolo, Kulon Progo roads. His areas of expertise are Motorcycle Business Engineering (TBSM), Automotive Light Vehicle Engineering (TKRO), Computer Network Engineering (TKJ). SMK Muhammadiyah Sentolo continues to carry out the process to improve and develop itself. One of them is by creating a Plus Program with the aim of preparing graduates who have resilience and independence. One of the plus programs is agroindustry Therefore, it is necessary to introduce agricultural processing methods using technology, especially technology that supports the fertilization process using liquid fertilizer. This Community Service Activity will introduce the use of fertilization technology using liquid media with the help of drones. The result of this service activity is that students know that the use of drones to help the fertilization process is meaningful for farmers, so students feel eager and enthusiastic to learn how to use the drone. On the farmer's side, they feel that the presence of drones applied by SMK Muhamaadiyah Sentolo students is a new hope that will be useful for helping farmers
Pendampingan desa wisata Bukit Roso Wulan dalam implementasi promosi on line berbasis facebook berbentuk video Poerwanto, Eko; Suhanto, Suhanto
KACANEGARA Jurnal Pengabdian pada Masyarakat Vol 5, No 2 (2022): Juli
Publisher : Institut Teknologi Dirgantara Adisutjipto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.28989/kacanegara.v5i2.1022

Abstract

Kegiatan Bisnis/Industri Wisata merupakan kegiatan yang cukup kompleks dan harus terintegrasi dari seluruh komponen yang ada. Kegiatan Promosi merupakan salah satu komponen yang cukup penting dalam Industri Wisata. Facebook merupakan sarana/media sosial  untuk komunikasi antar manusia, dan media sosial ini dapat digunakan untuk sarana promosi yang efektif dan efisien untuk mengenalkan obyek wisata “Bukit Roso Wulan”. Untuk mengemas konten promosi wisata sehingga menarik bagi pengunjung, perlu dibuatkan promosi dalam bentuk video. Mengingat keterbatasn SDM yang ada penting dilakukan pelatihan dan pendampingan desa wisata “Bukit Roso Wulan”, sehingga menjadi tujuan wisata yang menarik bagi seluruh masyarakat. Kegiatan Pengabdian Kepada Masyarakat ini diharapkan dapat mengaplikasikan konsep industri wisata di Desa Turunan, Girisuko, Panggang, Kabupaten Gunungkidul. Kegiatan selama PKM berlangsung dapat di lihat di Youtube ITDA dengan Link : https://youtu.be/1-YZhsyBmAA
Analysis of the Increase in Payload Capacity of Airbus A320 Aircraft at Radin Inten II International Airport Lampung Using an Improved Climb Approach Lukito, Indro; Budiono, Cyrilus Sukaca; Prakoso, Agung; Poerwanto, Eko; Mardwianta, Benedictus
Vortex Vol 6, No 1 (2025)
Publisher : Institut Teknologi Dirgantara Adisutjipto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.28989/vortex.v6i1.2802

Abstract

This study aims to analyze the increase in the payload capacity of the Airbus A320 at Radin Inten II International Airport Lampung using the Improve Climb approach. The Improve Climb approach focuses on optimizing the aircraft's climb path to enhance efficiency and payload capacity, particularly in airport conditions with high elevation and unfavorable weather. This study includes an analysis of the performance data of the Airbus A320. In addition, flight simulations were conducted taking into account factors such as wind speed, air temperature, and engine power. The results of this analysis are expected to provide recommendations regarding the optimization of flight schedules, passenger capacity, and improvements in operational efficiency at the airport. The results of the study indicate that by applying the Improve Climb approach, the aircraft's payload capacity can be increased by up to 3% without compromising safety. This opens up opportunities to increase the number of passengers.
Customer Satisfaction as an Intervening Variable in the Relationship Between Service Quality, Marketing Mix, and Customer Trust at Airports Lestiani, Melia Eka; Jannah, Tsamratul; Budiono, Cyrilus Sukaca; Poerwanto, Eko; Mardwianta, Benedictus
Vortex Vol 6, No 2 (2025)
Publisher : Institut Teknologi Dirgantara Adisutjipto

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.28989/vortex.v6i2.3700

Abstract

An airport is a facility where all stakeholders in the aviation industry converge, and its core product is “service,” which includes services for passengers, cargo, and aircraft. Customer satisfaction at airports is an essential outcome that must be delivered to all aviation industry stakeholders. This study aims to analyze the influence of service quality and marketing mix on customer trust, with customer satisfaction serving as an intervening variable at the airport. In reality, many airport service users still feel dissatisfied with both the services provided and the existing facilities. This is evident from various complaints submitted by users, indicating that they have not yet experienced satisfactory airport services. This research employs a quantitative method by distributing questionnaires to 100 respondents, consisting of domestic passengers at Minangkabau International Airport. The findings reveal that service quality and marketing mix have a significant partial and simultaneous effect on customer satisfaction. Furthermore, service quality, marketing mix, and customer satisfaction—both partially and simultaneously—have a significant influence on customer trust. However, the customer satisfaction variable is unable to mediate the relationship between service quality and the marketing mix toward customer trust.
Pengenalan Aspek Ergonomi pada Penataan Operasional Bongkar Muat Pasar Giwangan Yogyakarta Santoso, Prasidananto Nur; Astuti, Marni; Nurdin, Riani; Sullyartha, Esa Rengganis; Mauidzoh, Uyuunul; Gunawan, Gunawan; Poerwanto, Eko; Zabidi, Yasrin; Budiwidodo, Sidik
Mestaka: Jurnal Pengabdian Kepada Masyarakat Vol. 5 No. 2 (2026): APRIL 2026
Publisher : Pakis Journal Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58184/mestaka.v5i2.917

Abstract

Traditional markets act as food-logistics nodes with intensive unloading activities and manual handling that may increase musculoskeletal risk, internal congestion, and reduced accessibility due to layout and hygiene issues. This community-service paper is developed from a field assessment at Pasar Giwangan, Yogyakarta, conducted during 3 months (April–June 2020), and translated into an ergonomics socialization package, critical point mapping of circulation, and practical recommendations for loading and unloading operations. Key findings include heavy carrying (>50 kg) by informal porters with 2–5 repetitions per task, peak-hour congestion, reduced effective lane width caused by access configuration, and improper waste disposal that disturbs comfort and mobility. Proposed outputs include OSH ergonomics materials, strengthening handling aids (trolleys), and circulation/zonation adjustments for unloading activities.
Pembuatan Konten Promosi di Media Sosial untuk Produk Susu Kambing Etawa Zabidi, Yasrin; Nurdin, Riani; Astuti, Marni; Santoso, Prasidananto Nur; Gunawan, Gunawan; Rengganis, Esa; Mauidzoh, Uyuunul; Poerwanto, Eko; Budiwidodo, Sidik
Mestaka: Jurnal Pengabdian Kepada Masyarakat Vol. 5 No. 2 (2026): APRIL 2026
Publisher : Pakis Journal Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58184/mestaka.v5i2.922

Abstract

In today's digital era, promotional activities must follow digital marketing trends. One form of digital marketing is utilizing social media to promote products. The problem faced by the Etawa Goat Milk MSME was the lack of engaging promotional content on social media to introduce the product and increase its awareness among the wider public. Utilizing social media aims to make it easier for brands to connect with customers. The solution to this problem is to create engaging promotional content for publication on social media. The goal of this activity is to create engaging promotional content for publication on Instagram posts, stories, and reels, Facebook, TikTok, and WhatsApp.