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All Journal Jurnal Ilmiah Farmasi Pendidikan Biologi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Current Biochemistry Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Keperawatan BSI Adi Widya : Jurnal Pengabdian Masyarakat Pharmasipha Martabe : Jurnal Pengabdian Kepada Masyarakat Jurnal Farmasi Indonesia Jurnal Abdimas Mandiri INTEGRITAS : Jurnal Pengabdian Community Empowerment Jurnal Farmasi Proceeding Biology Education Conference Prosiding University Research Colloquium Journal Prima Health Science Herbal Medicine Journal SWAGATI: Journal of Community Service Jurnal Sains dan Kesehatan Advance Sustainable Science, Engineering and Technology (ASSET) Sabar : Jurnal Pendidikan Agama Kristen dan Katolik Jurnal Hukum, Administrasi Publik dan Negara
Desi Purwaningsih
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Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Dentistry Economics, Econometrics & Finance Education Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Journal : advance sustainable science engineering and technology asset

 1 
Comparing Conventional and Modern Methods for The Phycocyanin Extraction from Spirullina sp Dian Marlina; Desi Purwaningsih; Reny Pratiwi; Ryan Werytama Saputra; Widiastuti Setyaningsih; Supriyono Supriyono
Advance Sustainable Science Engineering and Technology Vol. 6 No. 3 (2024): May - July
Publisher : Science and Technology Research Centre Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/asset.v6i3.907

Abstract

Spirulina platensis, a blue-green algae abundant in tropical regions, is rich in minerals, vitamins, fibers, and pigments, with low nucleic acid content. It has unique chromoproteins called phycobiliproteins, notably phycocyanin, used in various applications. This study aims to optimize phycocyanin extraction using different solvents (distilled water and sodium phosphate buffer pH 6.7) and methods (freeze-thaw and sonication). Spirulina platensis biomass was extracted in both solvents, then some of them was freeze for 24 and 48 hours followed by thawing overnight. The other was sonicated for 2.5 minutes, 50 Hz then soaked for 1, 2, and 3 hours. All of the samples were centrifuged at 6000 rpm for 10 minutes and the absorbance was measured using a UV-Vis spectrophotometer at wavelengths of 280, 620, and 650 nm. with freeze-thawing for 48 hours yielded the highest phycocyanin concentration (0.55%), with a yield of 11.07 and purity of 0.21. Sonication improved phycocyanin concentration, yield, and purity significantly, yielding 1.108, 25.85, and 0.26, respectively.
Comparing Conventional and Modern Methods for The Phycocyanin Extraction from Spirullina sp Marlina, Dian; Purwaningsih, Desi; Pratiwi, Reny; Saputra, Ryan Werytama; Setyaningsih, Widiastuti; Supriyono, Supriyono
Advance Sustainable Science Engineering and Technology Vol. 6 No. 3 (2024): May - July
Publisher : Science and Technology Research Centre Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/asset.v6i3.907

Abstract

Spirulina platensis, a blue-green algae abundant in tropical regions, is rich in minerals, vitamins, fibers, and pigments, with low nucleic acid content. It has unique chromoproteins called phycobiliproteins, notably phycocyanin, used in various applications. This study aims to optimize phycocyanin extraction using different solvents (distilled water and sodium phosphate buffer pH 6.7) and methods (freeze-thaw and sonication). Spirulina platensis biomass was extracted in both solvents, then some of them was freeze for 24 and 48 hours followed by thawing overnight. The other was sonicated for 2.5 minutes, 50 Hz then soaked for 1, 2, and 3 hours. All of the samples were centrifuged at 6000 rpm for 10 minutes and the absorbance was measured using a UV-Vis spectrophotometer at wavelengths of 280, 620, and 650 nm. with freeze-thawing for 48 hours yielded the highest phycocyanin concentration (0.55%), with a yield of 11.07 and purity of 0.21. Sonication improved phycocyanin concentration, yield, and purity significantly, yielding 1.108, 25.85, and 0.26, respectively.
Microencapsulation of Phycocyanin from Spirulina platensis by Freeze-Drying: Optimization of Maltodextrin–Soy Protein Matrices for Enhanced Stability and Antioxidant Functionality Marlina, Dian; Saputra, Ryan Werytama; Aji Prasetyo, Takad Bagas; Muhamad Ansory, Hery; Aisiyah, Siti; Purwaningsih, Desi
Advance Sustainable Science Engineering and Technology Vol. 8 No. 2 (2026): February-April
Publisher : Science and Technology Research Centre Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/asset.v8i2.2726

Abstract

Phycocyanin, a natural blue pigment from Spirulina platensis, exhibits strong antioxidant activity but is highly unstable under light, heat, and pH variations, limiting its practical applications. This experimental study addresses the lack of systematic optimization data on maltodextrin–soy protein isolate (SPI) wall matrices for phycocyanin microencapsulation via freeze-drying. Phycocyanin was extracted using phosphate buffer and encapsulated at different maltodextrin:SPI ratios (9:1, 8:2, and 7:3). Each formulation was analyzed in triplicate (n = 3) for encapsulation efficiency (EE), phycocyanin retention, moisture content, particle size, and antioxidant activity (DPPH assay). The 8:2 ratio exhibited the best performance with EE of 88.5%, phycocyanin content of 0.710 mg·mL⁻¹, and particle size of 70.2 µm. Moderate antioxidant activity was observed (IC₅₀ = 102.29 ppm). ANOVA confirmed that the polymer ratio significantly affected all parameters (p < 0.05). Overall, the optimized maltodextrin–SPI microcapsules enhanced the stability and antioxidant functionality of phycocyanin under laboratory conditions, supporting their potential application as bioactive ingredients in functional food and pharmaceutical formulations.
Co-Authors Aji Prasetyo, Takad Bagas Aji Wahyu Wahiddatul Djakiah Ana Indrayati Aris Tri Jaka Harjanta, Aris Tri Ayu anindia Ayu Rachmawati Bambang Agus Herlambang, Bambang Bowo Sugiharto Budi, Ikhsan Destik Wulandari Dewi Ekowati Dwi Ningsih Erna Irawan, Erna Febrian Murti Dewanto Ferina Dyah Prastiyanti Fitriana Noor Khayati Fradine, Chossy Harlita Harlita Haryo Kusumo Heryani, Monalisa I MADE ARTIKA I Made Artika Irawan, Ira Ismi Rahmawati Ismi Rahmawati Isna Jati Asiyah Juliana, Dara Kristina Simanjuntak, Kristina Simanjuntak Kusumo, Haryo Maharani, Novita Mandasari, Mitha Oktavia Marlina, Dian Mega Novita Mega Novita Muhamad Ansory, Hery Ningsih, dwi - Nisa, Zaitun Pradana, Arsita Pradana, Arsitya Pratami Djasfar, Seftiwan Putra Djonesti, wilfadri Rahmawati, Ismi Rahmawati, Ismi - Reny Pratiwi Reny Pratiwi Retno Yulianti Rizky Bimantara HA Ryan Werytama Saputra Saputra, Ryan Werytama Setyaningsih, Widiastuti Simanjuntak, Kristina Siti Aisiyah Sri Hayati, Sri Sri Rejeki Handayani Sri Rejeki Handayani, Sri Rejeki Suharyanto Suharyanto Supriyono Supriyono Supriyono Supriyono Tanzila Rahmi Tita Novarini Tiwuk Susantiningsih Tri Panji Turahman, Taufik Turahman, Taufik Vicky Agus Arwanda Widiastuti Setyaningsih Wulandari, Destik ‪Arlina Dhian Sulistyowati‬