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All Journal Jurnal Ilmiah Farmasi Pendidikan Biologi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Current Biochemistry Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Keperawatan BSI Adi Widya : Jurnal Pengabdian Masyarakat Pharmasipha Martabe : Jurnal Pengabdian Kepada Masyarakat Jurnal Farmasi Indonesia INTEGRITAS : Jurnal Pengabdian Community Empowerment Jurnal Farmasi Proceeding Biology Education Conference Journal Prima Health Science Herbal Medicine Journal SWAGATI: Journal of Community Service Jurnal Sains dan Kesehatan Advance Sustainable Science, Engineering and Technology (ASSET) Sabar : Jurnal Pendidikan Agama Kristen dan Katolik Jurnal Hukum, Administrasi Publik dan Negara
Desi Purwaningsih
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Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Dentistry Economics, Econometrics & Finance Education Electrical & Electronics Engineering Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Journal : Advance Sustainable Science, Engineering and Technology (ASSET)

 1 
Comparing Conventional and Modern Methods for The Phycocyanin Extraction from Spirullina sp Dian Marlina; Desi Purwaningsih; Reny Pratiwi; Ryan Werytama Saputra; Widiastuti Setyaningsih; Supriyono Supriyono
Advance Sustainable Science Engineering and Technology Vol. 6 No. 3 (2024): May - July
Publisher : Science and Technology Research Centre Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/asset.v6i3.907

Abstract

Spirulina platensis, a blue-green algae abundant in tropical regions, is rich in minerals, vitamins, fibers, and pigments, with low nucleic acid content. It has unique chromoproteins called phycobiliproteins, notably phycocyanin, used in various applications. This study aims to optimize phycocyanin extraction using different solvents (distilled water and sodium phosphate buffer pH 6.7) and methods (freeze-thaw and sonication). Spirulina platensis biomass was extracted in both solvents, then some of them was freeze for 24 and 48 hours followed by thawing overnight. The other was sonicated for 2.5 minutes, 50 Hz then soaked for 1, 2, and 3 hours. All of the samples were centrifuged at 6000 rpm for 10 minutes and the absorbance was measured using a UV-Vis spectrophotometer at wavelengths of 280, 620, and 650 nm. with freeze-thawing for 48 hours yielded the highest phycocyanin concentration (0.55%), with a yield of 11.07 and purity of 0.21. Sonication improved phycocyanin concentration, yield, and purity significantly, yielding 1.108, 25.85, and 0.26, respectively.
Comparing Conventional and Modern Methods for The Phycocyanin Extraction from Spirullina sp Dian Marlina; Desi Purwaningsih; Reny Pratiwi; Ryan Werytama Saputra; Widiastuti Setyaningsih; Supriyono Supriyono
Advance Sustainable Science Engineering and Technology Vol. 6 No. 3 (2024): May - July
Publisher : Science and Technology Research Centre Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/asset.v6i3.907

Abstract

Spirulina platensis, a blue-green algae abundant in tropical regions, is rich in minerals, vitamins, fibers, and pigments, with low nucleic acid content. It has unique chromoproteins called phycobiliproteins, notably phycocyanin, used in various applications. This study aims to optimize phycocyanin extraction using different solvents (distilled water and sodium phosphate buffer pH 6.7) and methods (freeze-thaw and sonication). Spirulina platensis biomass was extracted in both solvents, then some of them was freeze for 24 and 48 hours followed by thawing overnight. The other was sonicated for 2.5 minutes, 50 Hz then soaked for 1, 2, and 3 hours. All of the samples were centrifuged at 6000 rpm for 10 minutes and the absorbance was measured using a UV-Vis spectrophotometer at wavelengths of 280, 620, and 650 nm. with freeze-thawing for 48 hours yielded the highest phycocyanin concentration (0.55%), with a yield of 11.07 and purity of 0.21. Sonication improved phycocyanin concentration, yield, and purity significantly, yielding 1.108, 25.85, and 0.26, respectively.
Co-Authors Aji Wahyu Wahiddatul Djakiah Ana Indrayati Aris Tri Jaka Harjanta, Aris Tri Ayu anindia Ayu Rachmawati Bambang Agus Herlambang, Bambang Bowo Sugiharto Destik Wulandari Dewi Ekowati Dwi Ningsih Erna Irawan, Erna Febrian Murti Dewanto Ferina Dyah Prastiyanti Fradine, Chossy Harlita Harlita Haryo Kusumo Heryani, Monalisa I Made Artika I MADE ARTIKA Irawan, Ira Ismi Rahmawati Ismi Rahmawati Isna Jati Asiyah Juliana, Dara Kristina Simanjuntak, Kristina Simanjuntak Kusumo, Haryo Maharani, Novita Mandasari, Mitha Oktavia Marlina, Dian Mega Novita Mega Novita Ningsih, dwi - Nisa, Zaitun Pradana, Arsita Pradana, Arsitya Rahmawati, Ismi Rahmawati, Ismi - Reny Pratiwi Rizky Bimantara HA Ryan Werytama Saputra Simanjuntak, Kristina Siti Aisiyah Sri Hayati, Sri Sri Rejeki Handayani Sri Rejeki Handayani, Sri Rejeki Suharyanto Suharyanto Supriyono Supriyono Tanzila Rahmi Tita Novarini Tiwuk Susantiningsih Tri Panji Turahman, Taufik Turahman, Taufik Vicky Agus Arwanda Widiastuti Setyaningsih Wulandari, Destik