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All Journal HAYATI Journal of Biosciences Jurnal Veteriner Microbiology Indonesia Pharmaciana: Jurnal Kefarmasian Jurnal Matematika & Sains Journal of Mathematical and Fundamental Sciences Jurnal Sains dan Teknologi Farmasi Jurnal Media Gizi Indonesia (MGI) INDONESIAN JOURNAL OF PHARMACY Jurnal Komputer Terapan ANNALES BOGORIENSES Jurnal Sisfokom (Sistem Informasi dan Komputer) FITOFARMAKA: Jurnal Ilmiah Farmasi Jurnal Respirasi (JR) Darmabakti Cendekia: Journal of Community Service and Engagements Jurnal Layanan Masyarakat (Journal of Public Service) Narra J Jurnal Puruhita MEDICINUS Journal of Community Engagement in Health Annales Bogorienses
HENI RACHMAWATI
School of Pharmacy, Institute of Technology Bandung. Jl. Ganesha, 10, Bandung 40132
Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary Other

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The Changes of Immunohistochemistry in Lung Tissues, Surfactant Protein-D, eNOS, and NO in Mice Exposed to Essential Oil Vapor Veterini, Anna Surgean; Putri, Herdiani Sulistyo; Dwiyatna, Archie Arman; Rahmah, Ainur; Satuman, Satuman; Rachmawati, Heni; Meirawan, Rizky Fajar; Sulistiawan, Soni Sunarso
Jurnal Respirasi Vol. 11 No. 2 (2025): May 2025
Publisher : Faculty of Medicine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jr.v11-I.2.2025.123-130

Abstract

Introduction: The use of essential oils in aromatherapy is widespread. However, few studies have explored the effects of smoke from the evaporation of commonly used essential oils. While essential oils are promoted for various benefits, prolonged exposure to inhaled particles from essential oil smoke may pose potential health risks. This study aimed to examine the effects of essential oil vapors on mice. Methods: This was an experimental study investigating the effects of different treatments on lung immunohistopathology, endothelial nitric oxide synthase (eNOS) expression, serum nitric oxide (NO) levels, and serum surfactant protein-D (SP-D) as an inflammation marker in mice. A total of 40 adult male Mus musculus mice (25–30 g) were randomly divided into four groups. Inflammation models were established by exposing the mice to a gas mixture containing vegetable glycerin, propylene glycol, and vitamin E acetate solution. Following inflammation induction, the mice received seven-day interventions with 0.9% NaCl solution, Eucalyptus globulus essential oil (EgEO), and citronella essential oil (CtEO), alongside an untreated inflammatory group. Results: The CtEO group intervention showed significant increases in eNOS expression (P=0.001) but no significant increase in NO compared to the other groups. The correlation analysis of eNOS expression in lung cells, serum NO, and SP-D levels was not significant, P>0.05 (p=0.160; p=0.115; p=0.234). Conclusion: Gas intervention containing 100% oxygen (O2) and CtEO steam increased eNOS expression on the immunohistochemistry (IHC) examination of mice.
PENGEMBANGAN SISTEM INFORMASI CAGAR BUDAYA (SITARI) SEBAGAI UPAYA PELESTARIAN BUDAYA DI PROVINSI RIAU Dewi, Meilany; Novayani, Wenda; Rachmawati, Heni; Hidayat, Erzi; Zain, Muhammad Mahrus
Jurnal Komputer Terapan Vol 11 No 1 (2025): Jurnal Komputer Terapan
Publisher : Politeknik Caltex Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35143/jkt.v11i1.6495

Abstract

Awareness of the importance of cultural preservation should be a fundamental concern for every Indonesian citizen, particularly in the face of the rapid cultural influences brought about by globalization and advancements in internet technology. This is especially relevant for the people of Riau Province, which is rich in historical heritage and cultural diversity. The development of SITARI (Sistem Informasi Cagar Budaya Riau) represents a strategic initiative aimed at preserving the cultural assets of Riau Province through the facilitation of cultural heritage reporting, heritage data collection, and 360-degree virtual tours.During the development process, a Focus Group Discussion (FGD) was conducted involving representatives from relevant government agencies across the 12 regencies and municipalities in Riau Province. The objective was to identify existing challenges and gather input regarding system development needs. In parallel, a public survey was carried out to capture community perspectives on the difficulties faced in reporting suspected cultural heritage objects. The system was developed using the waterfall model approach. Evaluation results indicate that the SITARI application operates effectively and performs optimally without errors. It is deemed relevant in supporting cultural literacy, heritage preservation, and the promotion of tourism in Riau. The user interface and layout received positive feedback, indicating potential for further development. Notably, the 360-degree Virtual Tour feature was highly appreciated. The application also adheres to OWASP security standards, with minor low-risk vulnerabilities identified for future improvement.
Development of a Fluorescence Immunoassay Based on Curcumin Carbon Dots-labeled IgY Antibodies for SARS-CoV-2 Detection Rachmadani, Nisa Amanda; Soetomo, Meilisa Keizia; Taharuddin, Audrey Angelina Putri; Santika, Arum Sinda; Abdillah, Oktaviardi Bityasmawan; Permatasari, Fitri Aulia; Iskandar, Ferry; Rachmawati, Heni; Fibriani, Azzania
HAYATI Journal of Biosciences Vol. 33 No. 1 (2026): January 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.1.44-57

Abstract

Carbon dots (CDs) are widely utilized in biomedical applications as fluorescent labels for imaging and diagnostics due to their excellent biocompatibility and superior optical properties. These advantages often make CDs a substitute for organic fluorescent dyes, which suffer from low emission intensity and poor photostability when interacting with biomolecules. Moreover, carbon-based materials are eco-friendly and can be synthesized from natural sources, such as curcumin, a chromophore compound abundantly available in Indonesia. Therefore, this study conducted a preliminary investigation on curcumin CDs-labeled IgY antibodies (IgY-cur CDs) for fluorescence immunoassay of SARS-CoV-2 in rapid test applications. The synthesis of the fluorescent label involved a carbodiimide coupling reaction using EDC/NHS agents to conjugate IgY antibodies with curcumin CDs. The IgY-cur CDs conjugate was confirmed to detect antigens through FRET immunosensor mechanisms, showing a significant increase in fluorescence intensity with increasing antigen concentrations (p < 0.05), with a minimum sample concentration of 10 ng. Furthermore, the IgY-CDs cur conjugate was applied as a reporter in a fluorescence-based LFIA using a sandwich assay format. The test strip successfully detected synthetic multiepitope SARS-CoV-2 antigens with an estimated detection limit of 54.28 µg and nasopharyngeal samples from confirmed COVID-19 patients within 35 minutes of operation. The test strip was evaluated to be stable under cold storage at 4°C for up to 3 weeks. In conclusion, curcumin CDs-labeled IgY antibodies demonstrate promising potential for further development as fluorescent labels in rapid diagnostic applications targeting SARS-CoV-2.
Performance Analysis of Classification Models in Multiclass Facial Expression Recognition Based on Eigenface Features Yulina, Syefrida; Rachmawati, Heni
Jurnal Sisfokom (Sistem Informasi dan Komputer) Vol. 12 No. 3 (2023): NOVEMBER
Publisher : ISB Atma Luhur

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32736/sisfokom.v12i3.1742

Abstract

Facial Expression Recognition (FER) is currently widely explored by researchers in the field of Computer Vision. The application of Machine Learning and Deep Learning methods is useful in developing an intelligent system that is accurate in recognizing facial expressions such as emotions. This is inseparable from the type of dataset and classification method used which certainly affects the desired results. To choose the right method, it is necessary to compare the performance of these methods. This study focuses on comparing the performance results of four classification methods namely, Convolutional Neural Network (CNN), Support Vector Machine (SVM), K-Nearest Neighbor (KNN), Naïve Bayes Classifier (NBC) on a multiclass dataset for seven classes of facial emotion labels based on Eigenface feature selection uses the Personal Component Analysis (PCA) algorithm. The test parameters used to perform method comparisons are accuracy, recall, precision, f1-score, as well as the Receiving Operating Characteristic (ROC) and Area Under Curve (AUC) curves. The results of the analysis state that the SVM method has the highest accuracy value, while other methods show varying performance based on recall, precision, f1-score, and ROC and AUC analysis. This research was conducted on the FER 2013 dataset which showed that the classification method tested had quite good performance according to the test parameters.
DNA Condensation Study of Fully Synthesized Lipopeptide-Based Transfection Agent for Gene Delivery Vehicle Tarwadi, Tarwadi; Rachmawati, Heni; Kartasasmita, Rahmana E.; Pambudi, Sabar; Arbianto, Alfan Danny; Restiani, Dewi Esti; Asyarie, Sukmadjaja
Annales Bogorienses Vol. 22 No. 2 (2018): Annales Bogorienses
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The main requirement of transfection agent has to condense DNA in nanoparticle size, protect the DNA from nucleases and other degrading enzymes during its transport in cell cytoplasm and nucleus and should not toxic to target cells. In this research, lipopeptide composed of palmitoyl (C-16) and short peptide sequence have been designed fully synthesized and tested to DNA condensation capability and toxicity. The DNA condensation study was performed using EtBr exclusion assay and cytotoxicity determination was carried out by colorimetric MTT assay. It was revealed that lipopeptide-based transfection agent of Pal-CKKHH and Pal-CKKHH-YGRKKRRQRRR-PKKKRKV condensed DNA molecules efficiently. The lipopeptide was less toxic compared to Lipofectamine and Poly-L-Lysine, that shown by 90% of CHO-K1 cells remained viable when they were treated with 4.36 µM Pal-CKKHHYGRKKRRQRRR-PKKKRKV. Meanwhile, there were only ~75% and 80% of CHO-K1 viable cells when it was treated with PLL and Lipofectamine®2000, respectively. Moreover, cell viability of HepG2 was ~ 75% after treated with 2.18 µM of Pal-CKKHH-YGRKKRRQRRR-PKKKRKV and decreased to ~65% when the lipopeptide concentration increased to 8.72 M. In summary, the synthesized lipopeptide condenses DNA molecules efficiently, less toxic than Lipofectamine®2000 and PLL and has possibility to be explored as a non-viral gene delivery vehicle.
Overproduction and Purification of Soluble Recombinant Human Granulocyte Colony Stimulating Factor in Escherichia coli Using Thioredoxin as Fusion Agustiyanti, Dian Fitria; Retnoningrum, Debbie Sofie; Rachmawati, Heni; Fuad, Asrul Muhamad
Annales Bogorienses Vol. 21 No. 1 (2017): Annales Bogorienses
Publisher : BRIN

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Abstract

Recombinant human Granulocyte Colony Stimulating Factor (G-CSF) has been produced in a soluble form in Escherichia coli BL21 (DE3) as a fusion protein. The open reading frame of G-CSF was synthetically constructed in previous work and was codon optimized for best expression in E. coli. In this research, the gene was fused to thioredoxin (Trx) at the N-terminal in pET32 vector. The purpose of this research was to optimize the overproduction and purification processes to obtain high yield recombinant protein in soluble form, and to characterize the Trx-G-CSF fusion protein. Overproduction was performed using IPTG induction method for 3 and 6 hours. The protein was purified by Ni-NTA affinity chromatography and separated using gradient concentration of imidazole. The purified protein was then characterized by SDS-PAGE and Western Blot analysis. Further, enterokinase was used to separate G-CSF from the fusion protein. The purified form of G-CSF was subsequently characterized using Western Blot and mass spectrometry using MALDI-TOF. The results showed that the fusion protein was successfully produced in soluble part as much as 48.25% were obtained after 3 hours of induction. The yield of fusion protein was 67.37% from total protein (229.65 mg protein/L culture). The Western Blot analysis showed the G-CSF band at around 18.6 kDa. Mass spectrometry with MALDI-TOF/ TOF revealed that 25.86% of amino acid residue was recognized as part of human G-CSF sequence.
Co-Authors Abdillah, Oktaviardi Bityasmawan Agustiyanti, Dian Fitria Agustiyanti, Dian Fitria Alfan Danny Arbianto Ali Iqbal Tawakal Ali Iqbal Tawakal Amalia, Riezki Ambarwati, Rini Amirah Adlia Amirah Adlia Amirah Adlia Anna Smdyah Putri Anna Surgean Veterini Annis Catur Adi Arbianto, Alfan Danny Arbianto, Alfan Danny Arini Setiawati Asrul Muhamad Fuad Asrul Muhamad Fuad, Asrul Muhamad Atsarina Anindya Bambang Wahjuprajitno Citra Ariani Edityaningrum, Citra Damar Rasti Adhika Deandra Ardya Regitasari Deandra Ardya Regitasari DEBBIE SOFIE RETNONINGRUM DEBBIE SOFIE RETNONINGRUM Debbie Sofie Retnoningrum Dewi Esti Restiani Dian Fitria Agustiyanti Dwiyatna, Archie Arman Emyr Reisha Ishaura Estherina Juliana Marbun, Estherina Juliana Farapti Farapti Fathrizqita Aghnia Raudhany Fatona Suraya, Fatona Ferry Iskandar Fibriani, Azzania Hamzah Hamzah Herdiani Sulistyo Putri Hidayat, Erzi I Ketut Adnyana Irda Fidrianny Isaura, Emyr Reisha Iskandar, Fery Jessie S. Pamudji Kusnandar Anggadiredja Leonny Yulita Hartiadi Lili Fitriani Magdalena Yuni Kristanti Maharani, Dita Galuh Mahmud Aditya Rifqi Marlina Indriastuti Meirawan, Rizky Fajar Muhammad mahrus zain Nelly Setiawaty Nuthathai Sutthiwong Pambudi, Sabar Pambudi, Sabar Permatasari, Fitri A. Permatasari, Fitri Aulia Pricillia Sinambela Putri, Dea Mayang Yulia Rachmad Suhanda Rachmadani, Nisa Amanda Rachmat Mauludin Rahmah, Ainur Rahmana E. Kartasasmita Rahmana E. Kartasasmita Rasyidi, Mohammad Fahmi RATIH ASMANA NINGRUM Rehatta, Nancy Margarita Resmi, Juniar Kalpika Restiani, Dewi Esti Restiani, Dewi Esti Rika Hartati Rini Ambarwati S.Pd. M Kes I Ketut Sudiana . Sabar Pambudi Safira Prisya Dewi Sakinah Aljuffrie Salisa, Wizara Santika, Arum Sinda Sari, Ririn Andika Sarmanu, Sarmanu Satuman Satuman Shafiqah Adam Siti Fatimah Zahro Soetomo, Meilisa Keizia Sophi Damayanti Subijanto Marto Soedarmo Subijanto Marto Soedarmo Sukmadjaja Asyarie Sukmadjaja Asyarie Sukmadjaja Asyarie Sulistiawan, Soni Sunarso Syefrida Yulina, Syefrida Taharuddin, Audrey Angelina Putri Tarwadi Tarwadi Tarwadi, Tarwadi Tarwadi, Tarwadi Tjandrawati Mozef Tri Suciati Vidya Anggarini Rahmasari Vidya Anggarini Rahmasari Wenda Novayani Wibowo, Indra Widijiati Widijiati Widjiati Widjiati Widodo Jatim P Widodo Jatim Pudjirahardjo Widodo, Ferri Wijayanti, Ni PAD. Wizara Salisa Wizara Salisa Wizara Salisa Yeyet C. Sumirtapura YEYET CAHYATI Yogie Handoko