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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences The Journal of Experimental Life Sciences (JELS) Buletin Tanaman Tembakau, Serat & Minyak Industri Research Journal of Life Science Jurnal Pembangunan dan Alam Lestari Journal of Tropical Plant Protection AGRIVITA, Journal of Agricultural Science JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Penelitian Pertanian Tanaman Pangan Biosaintifika: Journal of Biology & Biology Education Jurnal Entomologi Indonesia Agro Bali: Agricultural Journal Buletin Tanaman Tembakau, Serat & Minyak Industri Jurnal HPT (Hama Penyakit Tumbuhan)
Bambang Tri Rahardjo
Departemen Hama Dan Penyakit Tumbuhan Fakultas Pertanian Universitas Brawijaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Control & Systems Engineering Decision Sciences, Operations Research & Management Environmental Science Immunology & microbiology Medicine & Pharmacology Neuroscience

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Isolation and Identification Of Triterpenoid Saponin From Baringtonia asiatica Kurz Seeds. Tanor, Meity N; Abadi, Abdul Latief; Rahardjo, Bambang Tri; Pelealu, Jantje
Journal of Tropical Life Science Vol 4, No 2 (2014)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.4.2.%x

Abstract

The study aimed to identify the content of the triterpenoid saponin compound from Barringtonia asiatica seeds collected from coastal of Malalayang beach, Manado, North Sulawesi. The method of extraction was the maceration with methanol solvent. The identification of the isolate was by thin layer chromatography, column chromatography, and GCMS. The phytochemistry test result of B. asiatica seed extract contained the compounds of alkaloid, saponin, and tannin. Then, the compound was separated by a thin layer chromatography method with a solvent system of methanol:chloroform:water. It produced three nodes that were spread around polar (Rf 0.24), semipolar (Rf 0.6) and non-polar (Rf 0.78) areas. Meanwhile, the process of column chromatography could only separate two chemical components namely semipolar (Rf 0.6) and polar (Rf 0.76). The identification with GCMS resulted in three compounds of Triterpenoid saponins, namely 2.4-bis-(1.1-dimethyl ethyl); 4-Dodecylphenol; and 2.6 bis-(1.1-dimethyl ethyl-4-methyl).
Classification of Fruit Flies In South Kalimantan Based on Morphology and Molecular Characters Pramudi, Muhammad Indar; Puspitarini, Retno Dyah; Rahardjo, Bambang Tri
Journal of Tropical Life Science Vol 3, No 3 (2013)
Publisher : Journal of Tropical Life Science

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Abstract

The result of identification from assemblies trapped by methyl eugenol and collection of fruit affected fruit flies at all location, there are seven specieses of fruitfly in all locations. Based on morphological identification, which analyzed UPGMA by using the CLAD 97 program, shows that the seventh species remain one group that consists of 5 sub groups. On the other hand, according to RAPD result, which analyzed UPGMA based on 20 DNA band characters, shows that the seventh species divided into two groups. The first group consists of B. umbrosa Fabricius, B. occipitalisis Bezzi, B. latifrons Hendell sub groups. The second group consists of Bactrocera carambolae Drew and Hancock, B. papayae Drew and Hancock, and B. albistrigata de Meijere, B. cucurbitae Coquillet sub groups. Sequencing result shows that homology of seventh fruitfly species, is 83 base pairs/bp (C), 101 bp (T), 265 bp (G), 420 bp (A), 432 bp (T), 600 bp (A) (appendixes 7). The length base pair for B. occipitalis, B. cucurbitae, B. albistrigata, B. carambolae, B. papayae, B. latifrons row is 615, 898, 570, 969, and 615 bp. An analysis result of morphology feature and RAPD method show distinction in group division and sub group, but the identification morphological and DNA of the seventh species fruitfly which is found are the similar.Keywords: Fruitfly, UPGMA, Morphoogy, RAPD, DNA
Co-Authors A. A. Ketut Aryawan A. A. Ketut Aryawan Abdul Latief Abadi Achadian, Etik Mar`ati Achmad Faisal Akbar Adistanaya, Radika Adrianto Marthinus Ndii Affandi, Rohmat Sayful Ahmad Muhlisin Ahmad Muhlisin Akhmad Rizali Annike Putri Damayanti Antika Anggraini Ayu Rosmayuningsih Ayu Rosmayuningsih Ayumi, Cicik Nur BANDUNG SAHARI Bobby Polii David Soetjipto Dede Durahman Dede Durahman Devi, Mia Prastika Fadloli, Ahmad Iqbal Rijal Faljila, Fanisa Fitriani, Ima Gatot Mudjiono Guntur Respyan Guntur Respyan Hagus Tarno Hagus Terno Hidayat, Mohamad Rizkiy Husni dan Zulfanazli Pudjianto Alfian Rusdy Ika Putri Utami Ika Rochdjatun Sastrahidayat Isna Kartika Wati Isnainy Dinul Mursyalati Yus Isnainy Dinul Mursyalati Yus Jami'at Dwi Elriyono Jantje Pelealu Januarisya, Muthia Adira Karisma Aditya Wardani Kristiana Sri Wijayanti Kurnia Paramita Sari Laraswati, Nur Adinda Liza Afifah Liza Afifah Liza Afifah Ludji Pantja Astuti Ludji Pantja Astuti Ludji Pantja Astuti Mahendra, Niko Manurung, Jihan Salmaa Afiifah Marianus Marianus Marwoto . Maspupah Huzni Maspupah Huzni Mawaddah, Rabbiana Meity Nelltje Tanor Mochammad Syamsul Hadi Mufidah Afiyanti Muhammad Indar Pramudi Muhammad, Faiz Nashiruddin Nur Angga Prayoga Nurlinda Nurlinda Nurul Aini Okty Ayu Lestari Praditya, Al Farriz Sandro Presti Mardiyani Purwaningtyas Presti Mardiyani Purwaningtyas, Presti Mardiyani PURNAMA HIDAYAT Putra, Satrya Adhimas Eka Putri Setya Rahmita Rahmawati, Alvina Eka Ratna Wulandari Retno Dyah Puspitarini Rina Rachmawati Rina Rachmawati Rina Rachmawati Rizki Nur Suci Rizki Puji Widiastuti Rizki Puji Widiastuti Rizky Septiyawati Ronny Pamuji Ronny Pamuji Rose Novita Sari Handoko Senoaji, Wasis Septiani, Rohma Wulan Silvi Ikawati Simatupang, Rusdi Ali Sabar Sri Karindah Sri Karindah Sumeru Ashari Syamsuddin Djauhari Syamsuddin Djauhari Syamsulhadi, Mochammad Taufiqurrahman, Achmad Fitriadi Teguh Yulianto, Teguh Tita Widjayanti Tita Widjayanti Toto Himawan Toto Himawan Yuliantoro Baliadi Zeni Ningrum Zeni Ningrum