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All Journal Lembaran Ilmu Kependidikan Rekayasa: Jurnal Penerapan Teknologi dan Pembelajaran Sainteknol : Jurnal Sains dan Teknologi Jurnal Biologi dan Pembelajarannya (JBP) Journal of Biology Education Unnes Science Education Journal BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Jurnal Penelitian Pendidikan IPA (JPPIPA) Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Biosaintifika: Journal of Biology & Biology Education Martabe : Jurnal Pengabdian Kepada Masyarakat Journal of Innovative Science Education Journal of Primary Education Jurnal Pengabdian Masyarakat Bumi Raflesia Abdimas Toddopuli: Jurnal Pengabdian Pada Masyarakat Berkala Penelitian Hayati Life Science Prosiding Seminar Nasional Pascasarjana Proceeding of International Conference on Science, Education, and Technology Prosiding Seminar Nasional Biologi Unnes Science Education Journal Biosaintifika: Journal of Biology & Biology Education Journal of Biology Education "Indonesian Journal of Devotion and Empowerment (IJDE) "
Enni Suwarsi Rahayu
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Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Physics Social Sciences Other

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Effect of BAP and Picloram on Shoot Induction (Musa acuminata Colla var. Mulu Bebek) Nurul Huda Am Zen Arief; Enni Suwarsi Rahayu; Furzani Pa’ee; Noor Aini Habibah
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 1 (2025): April 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i1.19605

Abstract

Musa acuminata Colla var. Mulu Bebek is a high-quality banana from Ternate, North Maluku. Its conventional cultivation faces challenges due to Fusarium wilt, reducing productivity. In vitro culture offers a solution for rapid, disease-free seedling production. This study aims to examine the effect of BAP and picloram on shoot induction using a CRFD at various concentrations (0–3 ppm) and evaluate the percentage of live explants, emergence time, and number and length of shoots and roots. Results show that 1 ppm picloram accelerates root emergence, while 0 ppm promotes the fastest shoot emergence, the longest shoot and root lengths. 1 ppm BAP increases shoot numbers. The interaction of picloram and BAP has a significant effect on the parameters of the fastest root emergence time, namely 1 ppm picloram + 0 ppm BAP, 1 ppm and 3 ppm, the fastest shoot emergence time, namely without the addition of PGR, and the longest shoot length, 0 ppm picloram + 2 ppm BAP. The combination of 0 ppm picloram + 1 ppm BAP is the optimal condition for a percentage of 100% live explants, the fastest time for root emergence, the highest number of shoots and roots, the longest shoot and root length and the optimal condition for the fastest time for shoot emergence is 0 picloram + 0 ppm BAP. This study provides insight into the role of BAP and picloram in inducing banana shoots that has not been reported before. The micropropagation media formula obtained in this study is an effort to improve the quality of healthy banana seedlings in the future.
Enhancing Plantlet Growth of Vanda floresensis Motes through Acclimatization Chamber, Chitosan Spraying, and Mixed Medium Enni Suwarsi Rahayu; Noor Aini Habibah; Yustinus Ulung Anggraito; Dinda Melati; Na Dame Maulibulung Sinaga; Furzani Pa’ee
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 3 (2025): November 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i3.33383

Abstract

The conservation of  Vanda floresensis Motes has been conducted using in vitro techniques and has produced a large number of plantlets. These plantlets require acclimatization, but the optimal method has not yet been found. The aims of the research were to develop an acclimatization protocol by verifying the effectiveness of an acclimatization chamber (AC), chitosan spraying, and mixed medium composition on plantlet growth.  The research was conducted in a screen house of Universitas Negeri Semarang, using a factorial randomized block design with three factors. The AC factor consisted of two levels: inside and outside the AC. The chitosan concentration factor consisted of five levels, namely 0.0, 1.5, 3.0, 4.5, and 6.0 ppm. The composition of the mixed medium consisted of three levels. The observed variables included the increase in number, length, and width of the leaf, total chlorophyll content, and wilted leaf. Data were analyzed by ANOVA and Duncan test. It can be concluded that the use of AC, chitosan spraying, and mixed medium composition was effective in increasing leaf growth and total chlorophyll content and reducing wilted leaf.  Spraying chitosan of 1.5 - 3.0 ppm with the use of AC and spraying chitosan of 1.5 ppm with a mixed medium of brick pieces, coconut fibre, and tree bark chips with a ratio of 1:2:1 resulted in the highest leaf growth and total chlorophyll content, and the lowest wilted leaf. The results of this study can be used as an acclimatization protocol for orchid propagation for conservation purposes.
Co-Authors Afifah, Diana Iffatul Afifah, Diana Iffatul Agus Setiowati, Agus Ahmad Agus Prasetyo Ainun Najib, Mega Rifqi Aisirotul Maisah Ajeng Mudaningrat Akbar, Bintang Faisal Albian Mubarak Amalia, Adninta Husnu Amalia, Isna Rizqi Amin Retnoningsih Amin Retnoningsih Andika, Erlangga Dwi Andreas Priyono Budi Prasetyo Ani Rusilowati Aprilanti, Harmita Ari Yuniastuti Ariesta, Putri Ariesta, Putri Asrizal Wahdan Wilsa Asyrofahnti, Novita Azfa, Zuraida Anissa Betty Shinta Indriani Bintang Faisal Akbar Cahyati, Wahyu Nilam Danissa Wirna Karmesti Desy Irmawati Dinda Melati Dyah Rini Indriyanti Edi Guhardja Ely Rudyatmi, Ely Erni Suharini Erni Suharini F. Putut Martin H.B., F. Putut Fadhila Isnahardiyanti Fahuwa, Istafta Dina Fauziyyah, Alyaa Fikri, Irvan Maulana Fitarahmawati Fitri Arum Sasi, Fitri Arum Furzani Pa’ee Furzani Pa’ee Ginanjar Puji Rahayu, Ginanjar Puji Hanifatul Umami Hapsari HERU SETIAWAN Hidayah, Laila Nur Hidayah Ibnul Mubarok Ika Puspita Sari Indradewa, Rhian Intan Kurniawati Karmesti, Danissa Wirna Kris D, Yermia Dita Krispinus Kedati Pukan Kumalasari, Mia Lady Rahmawati, Lady Lina Herlina Lina Herlina Lina Herlina Maghfiroh, Ana Nailatul Maghfiroh, Maila Shofa Maila Shofa Maghfiroh Maisah, Aisirotul Margareta Rahayuningsih Nugroho Edi K Mariyam, Siti Neng Martien Herna Susanti Melika Citra Tania Moh. Solehatul Mustofa Muhammad Abdul Jabar Muhammad Abdullah Muna, Khusniyyatul Mustofa , Moh. Solehatul Na Dame Maulibulung Sinaga Na Dame Maulibulung Sinaga Nabila, Chelsea Eka Putrista Naila Istianah Nana Kariada Trimartuti Nicky, Dhea Nihayah, Durrotun Ning Setiati Noor Aini Habibah Noor Aini Habibah Noor Aini Habibah Nugrahaningsih Nugrahaningsih Nugroho Edi Kartijono Nur Endah Assalma, Nur Endah Nur Hikmah Nur Kusuma Dewi Nur Kusuma Dewi Nurul Huda Am Zen Arief Parmin - Pramesti Dewi Prayogo, Gogo Priyanti Susanti Priyantini Widiyaningrum Putik Pribadi Putik Pribadi Putra, Ngurah Made Darma Putut Martin HB, Putut Martin Putut Marwoto Rahayu, Reny Retno Sri Iswari Rini K usumawardani Rio Candra, Rio Rohman, Farid Fadhlu Ruhama Desy, Ruhama Safira Pramudya Wardani Sari, Ika Puspita Sarie, Fitria Novita Sarwi Sarwi Sarwi Sarwi Satriyas Ilyas Sholeha, Siti Sigit Saptono Siti Fathonah Siti Fathonah Siti Harnina Bintari Siti Rochmah Sri Mulyani Endang Susilowati, Sri Mulyani Endang Sri Ngabekti Sriyadi Sriyadi Subiyati Sudarso - Sudarsono Sudarsono Sudarsono Sujatha Ramasamy Surya Diantina, Surya Susanti, Priyanti SUTIKNO Talitha Widiatningrum Tri Nurhidayah, Tri Tri Wahyu Setyaningrum, Tri Wahyu Vitradesie Noekent, Vitradesie Wakidah, Koriatun Widi Puji Astuti Widiatningrum, Talitha Widiatningrum Winarti, Yayuk Wiwi Isnaeni Yatin Mulyono Yayuk Winarti, Yayuk Yustinus Ulung Anggraito Yustinus Ulung Anggraito Zahra, Oktavina Nikmatuz Zumroh, Nur Zumroh, Nur