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Optimization of In Vitro Conservation Protocol of Carica pubescens Lenne & K. Koch through Medium Concentration, Temperature and Irradiation Duration Decrease Enni Suwarsi Rahayu; Noor Aini Habibah
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 1 (2016): March 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i1.5371

Abstract

Carica pubescens Lenne K. Koch, an endemic species in Dieng mountains, must be conserved. The in vitro conservation has been developed, but sub-culture period needs to be extended. This study aimed to obtain a more efficient in vitro conservation protocol of C. pubescens. The research was carried out experimentally by using a completely randomized factorial design with three factors, namely decreasing in storage medium concentration (75% and 50% of MS medium), temperature (4oC and 8°C), and irradiation duration (8 hours/day and 16 hours/day). Shoots were kept in the storage medium for 6, 9 and 12 months, then their viability were tested by growing them in the regeneration medium. Data were analyzed by Analyses of Variance and Least Significant Difference Test. The results showed that medium concentration of 50% of MS, the temperature of 8 °C, and 16 hours/day of irradiation were able to suppress the C. pubescens growth in vitro storage for six months and could maintain its viability in the regeneration medium. Based on these results the medium concentration of 50% of MS, the temperature of 8 °C and 16 hours/day irradiation can be used for in vitro conservation of C. pubescens without sub-culture for six months. How to CiteRahayu, E., Habibah, N. (2016). Optimization of In Vitro Conservation Protocol of Carica pubescens Lenne K. Koch through Medium Concentration, Temperature and Irradiation Duration Decrease. Biosaintifika: Journal of Biology Biology Education, 8(1), 85-93.
Comparison of Nutritional Content of Mangifera indica L. var. Wirasangka with Three Other Varieties in Tegal Regency Enni Suwarsi Rahayu; Amin Retnoningsih; Muhammad Abdullah; Priyanti Susanti
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 3 (2020): December 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i3.26728

Abstract

Mangifera indica L. var. wirasangka or wirasangka mango, a local plant in Tegal Regency, has a low population because it is rarely planted by people as its unknown nutritional content. To increase people's interest in planting, it is necessary to know the nutritional content of wirasangka mango. The aim of the study was to compare the nutritional content of wirasangka mango with three other varieties in Tegal Regency. This study used a one-factor completely randomized design. The factor was the mango variety which included six varieties, namely the varieties of wirasangka from three areas, okyong, tengkoeh, and golek. The variables observed were content of water, fiber, fructose, β-carotene, potassium and iron, as well as vitamin C and antioxidant activity. Normally distributed data were analyzed using One-Way Analysis of Variance and Least Significant Difference (LSD) test. Besides, the non-normally distribution data were analyzed using Kruskal Walis and continued with post-hoc test, i.e. Mann-Whitney. The results showed that the levels of water, dietary fiber, fructose, β-carotene, and iron were significantly different at the significance level of 0.1. Wirasangka mango has a higher fiber, and fructose content, but lower of β-carotene and iron content compared to okyong, tengkoeh and golek mangoes. The levels of vitamin C, potassium, and antioxidant activity of wirasangka mango were equivalent to the three other varieties studied. Thus the wirasangka mango has advantages which are expected to increase the interest of people in Tegal Regency to plant this variety. As a result, its population in the future will also increase.
KADAR VITAMIN DAN MINERAL DALAM BUAH SEGAR DAN MANISAN BASAH KARIKA DIENG (Carica pubescens Lenne&K.Koch) Enni Suwarsi Rahayu; Putik Pribadi
Biosaintifika: Journal of Biology & Biology Education Vol 4, No 2 (2012): September 2012
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v4i2.2273

Abstract

Penelitian bertujuan untuk membandingkan kadar vitamin A, vitamin C, fosfor, besi, dan kalsium dalam buah segar dan manisan basah Carica pubescens Lenne K. Koch (karika dieng) serta menentukan waktu perebusan optimal dalam proses pembuatan manisan karika yang tidak menurunkan kadar vitamin C secara signifikan. Kadar vitamin C dianalisis menggunakan titrasi yodium yacobs, kadar vitamin A diukur dengan spektronik-20, dan kadar mineral diukur dengan AAS. Data kadar vitamin dan mineral dianalisis menggunakan t-test, sedangkan waktu perebusan optimal dianalisis menggunakan Anava dan uji beda nyata terkecil. Hasil penelitian menunjukkan bahwa kadar vitamin A, vitamin C, fosfor, besi, dan kalsium pada lima merk manisan karika lebih kecil dibandingkan dalam buah karika segar. Waktu perebusan optimal adalah 10 menit.The study was aimed to compare the levels of vitamin A, vitamin C and the minerals i.e. phosphorus, iron and calcium in sweet preserved and fresh fruit of Dieng mountain papaya Carica pubescens Lenne K. Koch, and to determine the optimal boiling duration of the fruit in order to determine the preserved fruit processing technique. The level of vitamin C was analyzed using Jacob’s iodine titration, the level of vitamin A was analyzed using Spectronic 20, and the minerals were analyzed using AAS. The data of the contents of vitamins and minerals in sweet preserved and fresh fruit were analyzed using t-test, whereas the data of the optimum boiling time was analyzed using Anova and a least significant difference test. The results showed that the levels of vitamin A and vitamin C and minerals P, Fe and Ca at 5 brands of sweet preserved carica were lower than in carica fresh fruit. The optimum boiling time was 10 minutes to obtain the high vitaim C content.
Development of In Vitro Conservation Medium of Carica pubescens Lenne & K. Koch through Nutrients Concentration Reduction and Osmoregulator Addition Enni Suwarsi Rahayu; Noor Aini Habibah; Lina Herlina
Biosaintifika: Journal of Biology & Biology Education Vol 7, No 1 (2015): March 2015
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v7i1.3538

Abstract

Carica pubescens Lenne K. Koch  is a rare species that need to be conserved.  The research aim was to develop a slow growth method of in vitro conservation medium through determining some effects of nutrition decreasing availability in the conservation medium on growth and survival of explants. Establishing epicotyls reached from in vitro seed germination was grown on diluted basic medium of 75% MS (Murashige and Skoog), 50% MS, 25% MS, while osmoregulator compound of mannitol and sorbitol was added to the full MS medium in several concentrations. The treatments were arranged in a completely randomized design with three replications. The epicotyls were grown at storage medium for 12 and 16 weeks, then their survival were evaluated at regeneration medium and rooting medium.  The diluted basic medium and osmoregulator addition were evaluated for its influence in retarding the culture growth in terms of improved survival over the period of 16 weeks.  Data analyzed by one way analysis of variance and Duncan’s multiple range test. The results showed that the decreasing of nutrition concentration suppressed the growth of the epicotyls until 16 weeks after conservation. Epicotyls taken from 16 weeks after conservation could grow on the regeneration medium. The best  survival  was shown by the 75% MS, 50% MS and supplementing of  20 g/l mannitol treatments. Based on these results, 50% MS medium  is recommended  for storage C. pubescens for 16 weeks with no sub-culture.Carica pubescens Lenne K. Koch (karika dieng) merupakan tanaman yang langka sehingga perlu dilestarikan. Penelitian ini bertujuan untuk memperoleh medium penyimpanan in vitro dengan teknik pertumbuhan minimal dengan mengamati pengaruh penurunan ketersediaan nutrisi dalam medium terhadap penurunan pertumbuhan dan daya tumbuh eksplan.Eksplan berupa epikotil kecambah in vitro. Perlakuan penurunan ketersediaan nutrisi dilakukan melalui reduksi konsentrasi nutrisi dari medium Murashige Skoog (MS) dan penambahan osmoregulator (manitol dan sorbitol) dengan berbagai konsentrasi. Penelitian dilakukan dengan rancangan acak lengkap satu faktor dengan tiga ulangan. Epikotil dipelihara dalam medium penyimpanan selama 12 dan 16 minggu, kemudian dievaluasi daya tumbuhnya dengan memelihara dalam medium regenerasi dan medium pengakaran. Data dianalisis dengan analisis varians satu arah dan uji Duncan. Hasil penelitian menunjukkan bahwa penurunan kecepatan penyerapan nutrisi berpengaruh terhadap pertumbuhan eksplan. Epikotil yang telah disimpan selama 12 minggu dan 16 minggu dan ditumbuhkan kembali pada medium regenerasi masih dapat tumbuh dengan intensitas tertinggi pada perlakuan pengenceran 50% MS dan 75% MS, serta penambahan manitol 20 g/l. Komposisi medium ini dapat dimanfaatkan untuk penyimpanan karika dieng selama 16 minggu tanpa sub-kultur. Konsentrasi medium MS 50% direkomendasikan untuk digunakan dalam penyimpanan C. pubescens selama 16 minggu tanpa sub-kultur.
AN IDENTIFICATION OF ANGIOSPERMS IN KEMBANG NATURE RESERVE, JEPARA REGENCY Maila Shofa Maghfiroh; Enni Suwarsi Rahayu; Talitha Widiatningrum
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 10 No. 1 (2023): August
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v10i1.9703

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Based on the diversity of angiosperms which are widespread throughout the world and play an important role, identification is necessary, especially in flora reserves. This study aims to determine the type of angiosperms in the Kembang Nature Reserve, Jingotan Village, Kembang District, Jepara Regency. The method used in this research was the shipping method from Pal 2 to Pal 6 of the Kembang Nature Reserve cruise line using a purposive sampling technique. The results of the study found  that 30 angiosperms, 9 species from the monocot class and 21 species from the dicot class. The monocot class includes 9 species: Vanilla planifolia, Ottochloa nodosa, Zingiber zerumbet, Arenga pinata, Salacca zalacca, Tacca palmata, Costus speciosus, Amorhophallus variabili, and Amorphophallus muelerri Blume. 21 species belonging to the class of dicots, namely Tectona grandis, Clerodendrum japonicum, Lantana camara, Leea indica, Sauropus androgynus, Elephantopus sp., Paederia foetida, Cyclea barbata, Anacardium occidentale, Dracontomelon dao, Mangifera indica, Teucapera cubeba, Cocephala, Clerodendrum infortunatum, Ficus septica, Artocarpus altilis, Cinnamomum iners, Dysoxylum densiflorum, Dysoxylum gaudichaudianum, and Dysoxylum densiflorum.
Implementasi Sumber Pembelajaran Berbasis Teks Multimodal Tema Hewan Endemik Hutan Manggrove Pascapandemi Covid19 : Studi Pustaka Ruhama Desy M; Sarwi Sarwi; Enni Suwarsi Rahayu; Dyah Rini Indriyanti
Prosiding Seminar Nasional Pascasarjana Vol. 3 No. 1 (2020)
Publisher : Pascasarjana Universitas Negeri Semarang

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Artikel ini bertujuan untuk melihat Implementasi Sumber Pembelajaran Berbasis Teks MultimodalTema Hewan Endemik Hutan Manggrove Pascapandemi Covid 19. Pemaparan ini berdasarkanterhadap 4 (empat) hal, yaitu (1) Sumber Pembelajaran Berbasis Teks Multimodal, (2) TeachingMaterial, (3) Hewan Endemik Hutan Manggrove, (4) Pembelajaran Pascapandemi Covid-19.Permasalahan yang muncul adalah Sumber Pembelajaran berbasis Multimodal dengan kondisiPascapandemi Covid-19. Proses kegiatan belajar-mengajar ditujukan untuk terwujudnya pesertadidik yang siap untuk bersaing di era revolusi industri 4.0 yang mewajibkan peserta didik mampumembangun dan menjadikan dunia dalam aspek pendidikan maupun masyarakat lebih maju. Sertamenjadikan peserta didik lebih meghargai serta dapat melestasrikan alam sekitar. Metode yangdigunakan yaitu literatur review atau studi pustaka, dengan melakukan review dari 230 artikelIlmiah kemudian di reduksi menjadi 107 artikel selanjutkan di reduski kedua menjadi 91 artikelkemudian reduksi ketiga menjadi 65 artikel dan reduksi keempat menjadi 33 artikel jurnal Ilmiahdari berbagai penerbit yang bereputasi baik.
Development of E-plantbook Learning Resources based on Mobile Learning Applications with a Scientific Approach Maila Shofa Maghfiroh; Enni Suwarsi Rahayu; Talitha Widiatningrum
International Conference on Science, Education, and Technology Vol. 8 (2022)
Publisher : Universitas Negeri Semarang

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Abstract

Abstract. The development of mobile learning application-based e-plantbook with a scientific approach is one of the efforts to improve the quality of an ideal learning resource for Plantae material in Senior High School. This study aims to develop mobile learning application-based e-plantbook with a scientific approach for Plantae material that was tested to measure the level of validity and practicality at SMA Negeri 1 Kembang, Jepara Regency, Central Java Province. This study used the Research and Development (R and D) method by S. Thiagarajani, Dorothy and Melvyn I. The results of the development were tested for validity by material experts and media experts, and tested for practicality by teachers and students. The results of validation by material experts was obtained 83% with very valid criteria, while the results of validation by media experts was obtained 95.6% with very valid criteria. The results of the validity of the e-plantbook were then tested for practicality by the teacher and 20 students which were obtained 91% and 82% with very good criteria. These results indicate that the e-plantbook is proven to be valid and practical as a source of student learning in SMA/MA.
Utilization of Mangrove Forest as a Source of Zoology Learning with a Stem Approach Ruhama Desy M; Sarwi Sarwi; Putut Marwoto; Enni Suwarsi Rahayu; Dyah Rini Indriyanti
International Conference on Science, Education, and Technology Vol. 7 (2021)
Publisher : Universitas Negeri Semarang

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Abstract

The purpose of this article is to present a study of mangrove forest function as a source of zoology learning with a STEM approach. Basically the use of the environment can create something active and effective in the implementation of teaching and learning. Mangrove forest have a variety information related to zoology so that it becomes one way to produce creative learning resources. The presentation of the article is based on 3 aspects, namely (1) Mangrove Forest, (2) Learning Resources, (3) STEM. Literature review or literature study is used as a method of this study, by reviewing scientific journal articles from various reputable publishers. Zoology learning with a STEM approach is one solution in improving the quality of the teaching and learning process, so as to be able to overcome problems that arise from students. STEM is useful for activating student motivation and making students able to think at higher levels. The application of learning involving mangrove forest that have zoological diversity and using the STEM approach can answer obstacles in special zoology learning.
Diversity of Edible Fruit Species in Gunung Leuser National Park Area, Aceh Tamiang Albian Mubarak; Enni Suwarsi Rahayu; Amin Retnoningsih
Jurnal Penelitian Pendidikan IPA Vol 9 No SpecialIssue (2023): UNRAM journals and research based on science education, science applic
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9iSpecialIssue.6164

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Aceh Tamiang Regency, located in the northern part of Sumatra Island with an area of 1.957.02 km², is part of the Leuser Ecosystem, famously known as the Gunung Leuser National Park-TNGL. The remarkable biodiversity in TNGL has earned it recognition as a national park and wildlife sanctuary. Threats to this biodiversity include illegal logging, land conversion, hunting, and wildlife trafficking. This study aimed to identify the diversity of edible fruit species in the Aceh Tamiang region of TNGL as an initial step in conservation and utilization efforts for fruit biodiversity. The genetic resources of edible fruit plants in this area hold significant potential for enhancing the quality and quantity of fruits in Indonesia. Hence, conservation and sustainable management are crucial for fruit plant development. The research was conducted in Bandar Pusaka, Tamiang Hulu, and Tenggulun. The exploration discovered 81 species of edible fruits from 27 families. Tenggulun had the highest number of species (61 species), followed by Bandar Pusaka (53 species) and Tamiang Hulu (44 species). Species like Mangifera odorata Griff., Garcinia mangostana L., Pometia pinnata J.R. Forst. & G. Forst., and others were found in all locations. The highest diversity index was in Tenggulun (HI 3.31), followed by Bandar Pusaka (2.88), and the lowest in Tamiang Hulu (2.39). Species evenness ranged from 0.63 to 0.81, indicating moderate and stable evenness levels. Species dominance was low, with values ranging from 0.007-0.009. 
Development of E-Modules Based on Problem Based Learning Assisted by Flipbook on Environmental Change Material in High School to Improve Problem Solving Ability Heru Setiawan; Enni Suwarsi Rahayu
Journal of Biology Education Vol. 13 No. 1 (2024): April 2024
Publisher : Universitas Negeri Semarang

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Abstract

The use of printed teaching materials in schools is less attractive to students and the ability to solve problems owned by students is relatively below average so that the basic competencies set have not been achieved. Advances in science and technology can be utilized to provide more varied teaching materials such as e-modules. The purpose of this study was to analyze the feasibility, practicality and effectiveness of e-modules in the learning process. The research design used is development (R&D) using the ADDIE model with the stages of analysis, design, development, implementation, and evaluation. The feasibility of e-modules is determined based on the validation of material experts and media experts. The practicality of e-modules is determined based on student responses and teacher responses. The effectiveness of e-modules is determined based on students' classical completeness and N-gain. The results showed that the material expert validation value was 90.27% (very feasible) and the media expert validation value was 92.85% (very feasible). The results of the e-module's practicality was that the teacher rated it as very practical. In the small-scale trial, all students rated practical. In the large-scale trial, 90.32% of students rated very practical and 9.68% of students rated practical. The effectiveness of the e-module was proven to be effective in improving students' problem solving ability with an N-gain of 0.61 with a medium category and a classical completeness of 93.33%.  
Co-Authors Adninta Husnu Amalia Afifah, Diana Iffatul Afifah, Diana Iffatul Agus Setiowati, Agus Ahmad Agus Prasetyo Ainun Najib, Mega Rifqi Aisirotul Maisah Akbar, Bintang Faisal Albian Mubarak Amalia, Isna Rizqi Amin Retnoningsih Andika, Erlangga Dwi Andreas Priyono Budi Prasetyo Ani Rusilowati Aprilanti, Harmita Ari Yuniastuti Arief, Nurul Huda Am Zen Ariesta, Putri Ariesta, Putri Asrizal Wahdan Wilsa Asyrofahnti, Novita Cahyati, Wahyu Nilam Desy Irmawati Dyah Rini Indriyanti Edi Guhardja Ely Rudyatmi, Ely Erni Suharini Erni Suharini F. Putut Martin H.B., F. Putut Fahuwa, Istafta Dina Fitri Arum Sasi, Fitri Arum Ginanjar Puji Rahayu, Ginanjar Puji Hanifatul Umami Hapsari HERU SETIAWAN Hidayah, Laila Nur Hidayah Ibnul Mubarok Ika Puspita Sari Indriani, Betty Shinta Intan Kurniawati Istianah, Naila Jabar, Muhammad Abdul Karmesti, Danissa Wirna Kris D, Yermia Dita Krispinus Kedati Pukan Kumalasari, Mia Lady Rahmawati, Lady Lina Herlina Lina Herlina Lina Herlina Maghfiroh, Maila Shofa Maila Shofa Maghfiroh Maisah, Aisirotul Margareta Rahayuningsih Nugroho Edi K Martien Herna Susanti Mudaningrat, Ajeng Muhammad Abdullah Muhammad Syarif Hidayat, Muhammad Syarif Muna, Khusniyyatul Mustofa , Moh. Solehatul Nana Kariada Trimartuti Nihayah, Durrotun Ning Setiati Noor Aini Habibah Nugrahaningsih Nugrahaningsih Nugroho Edi Kartijono Nur Endah Assalma, Nur Endah Nur Hikmah Nur Kusuma Dewi Nur Kusuma Dewi Parmin - Pa’ee, Furzani Pramesti Dewi Prayogo, Gogo Priyanti Susanti Priyantini Widiyaningrum Putik Pribadi Putik Pribadi Putra, Ngurah Made Darma Putut Martin HB, Putut Martin Putut Marwoto Ramasamy, Sujatha Reny Rahayu Retno Sri Iswari Rio Candra, Rio Ruhama Desy M Ruhama Desy M Sari, Ika Puspita Sarie, Fitria Novita Sarwi Sarwi Sarwi Sarwi Satriyas Ilyas Sholeha, Siti Sigit Saptono Siti Fathonah Siti Fathonah Siti Harnina Bintari Siti Rochmah Sri Mulyani Endang Susilowati, Sri Mulyani Endang Sri Ngabekti Subiyati Sudarso - Sudarsono Sudarsono Sudarsono Surya Diantina, Surya Susanti, Priyanti SUTIKNO Talitha Widiatningrum Tri Nurhidayah, Tri Tri Wahyu Setyaningrum, Tri Wahyu Vitradesie Noekent, Vitradesie Wakidah, Koriatun Widi Puji Astuti Widiatningrum, Talitha Widiatningrum Winarti, Yayuk Wiwi Isnaeni Yatin Mulyono Yayuk Winarti, Yayuk Yustinus Ulung Anggraito Zumroh, Nur Zumroh, Nur