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Deteksi coronavirus pada kelelawar di Kabupaten Lamongan Muhammad Badrut Tamam; Aisyah Hadi Ramadani; M. Ainul Mahbubillah
Livestock and Animal Research Vol 20, No 1 (2022): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1027.802 KB) | DOI: 10.20961/lar.v20i1.56667

Abstract

Objective: To obtain information about the presence of coronavirus in bats to find potential of new disease reservoir as well as not yet reported disease reservoir in Lamongan District. This research can be used by the government as a basis for planning and evaluating disease control programs and for researchers it can be used for vaccine and drug design, viral phylogenetic, analysis of viral distribution, and viral databases.Methods: Sampling was carried out at 3 points representing different habitat types. The first point is at Pucakwangi Cave Babat Lamongan, the second is at Paciran Lamongan beach area, and the last is at the Mantup Forest Lamongan. Sampling was carried out using a mist net with the help of local workers. The netted bats were caught and put in a bag. Parameters observed included gender, morphometry, species, and detection of the presence of coronavirus. Detection of the virus using the oropharyngeal swab method, then the RNA samples were tested by RT-PCR and sequencing. The primer used to detect the coronavirus is primer forward 5′-CACGCAACTTGTTGTAATGCGT CAGAGA-3′ and primer reverse 5′- CACGTGCTTTTGCAGGCACTATACGAC-3′.Results: Five species of bats obtained from 3 sampling locations namely Taphozous melanopogon, Chaerophon sp., Hipposideros sp., Cynopterus sp., Macroglossus sp. RT-PCR test of sample G1, G6, G8, P1, P2, P3, H1, H3, and H6, shows that no coronavirus from DNA sample amplified.Conclusions: Negative result of Coronavirus detection by molecular analysis is not an indicator of no potential of species Taphozous melanopogon, Chaerophon sp., Hipposideros sp., Cynopterus sp., and Macroglossus sp. as a Coronavirus reservoir. Increasing the number of samples and expansion of study area still needed to obtain more comprehensive data.
Pelatihan Budidaya Maggot dengan Memanfaatkan Sampah Organik Hasil Pemilahan di Kelurahan Blimbing, Lamongan Rofiatun Solekha; Fika Nur Fitriana Putri Bukhori; Silvi Wasi’atul Af'idah; Lailatus Fitri; Aisyah Hadi Ramadani
I-Com: Indonesian Community Journal Vol 2 No 3 (2022): I-Com: Indonesian Community Journal (Desember 2022)
Publisher : Fakultas Sains Dan Teknologi, Universitas Raden Rahmat Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (670.368 KB) | DOI: 10.33379/icom.v2i3.2001

Abstract

Pemilahan dan pengolahan sampah masih menjadi cara yang dibutuhkan masyarakat untuk mengurangi masalah lingkungan. Pemanfaatan sampah organic menjadi salah satu produk yang bernilai ekonomis menjadi salah satu solusi yaitu pemanfaatan sampah untuk pakan dalam budidaya maggot black soldier fly (BSF). Tim PHP2D Prodi Biologi Universitas Muhammadiyah lamongan memberikan solusi dengan memanfaatkan sampah organic untuk pakan budidaya maggot dalam upaya mengurai permasalahan di Kelurahan Blimbing, Kecamatan Paciran, Kabupaten Lamongan. Tujuan dari program ini adalah memberikan pendampingan dan pelatihan guna pembedayaan msyarakat dalam mengolah sampah organik bernilai eknonomis. Metode pelaksanaan kegiatan pengabdian ini diawali dengan penyuluhan atau sosialisasi, kemudian dilanjut dengan diadakannya pelatihan bersama dengan masyarakat setempat dalam pembuatan instalasi serta teknis budidaya black soldier fly (BSF). Hasil dari kegiatan ini adalah terciptanya produk maggot lalat black soldier fly (BSF) dalam produk kemasan siap jual. Seluruh kegiatan pelatihan berjalan dengan sukses. Proses budidaya telah melalui satu siklus hidup BSF dan dihasilkan produk maggot BSF yang siap untuk dipasarkan. Monitoring dan evaluasi program menunjukkan adanya timbal balik yang positif dari peserta.
Training on Separation of Organic and Inorganic Waste in Blimbing Village, Paciran Lamongan District Rofiatun Solekha; Nynda Ayu Nadira Savitri; Karin Alifia Rachmadani; Badriatul Musyarofah; aisyah hadi ramadani
Indonesia Berdaya Vol 4, No 2 (2023)
Publisher : Utan Kayu Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47679/ib.2023455

Abstract

Waste problems are becoming increasingly complex due to the lack of waste processing facilities, low public knowledge about waste management, and the rapid population growth in Paciran District, Lamongan Regency. This activity is carried out with the aim of utilizing it by sorting waste and processing it so that it can produce products. Trash that is scattered on the road is generally due to the low awareness of the surrounding community to dispose of waste in its place. Community service activities are carried out by socialization and training. The socialization carried out is to provide counseling to the community about how to dispose of waste and sorting household waste. Training was conducted for TPS3R officers on how to sort and use them. Organic waste obtained from food scraps is used as maggot feed, while inorganic waste is sold to collectors so that it can increase people's income in the Blimbing village. Apart from that, this activity also produced a BSF (Black Soldier Fly) installation, which is a place for maggot cultivation. It is hoped that in the future this community service activity can be carried out in a sustainable manner, so that the waste in the Blimbing sub-district can be utilized into something of economic value.
Peningkatan Pengetahuan dan Keterampilan Siswa Melalui Pengenalan Proses Penjernihan Air Secara Sederhana Muh. Shofi; Nurul Istiqomah; Aisyah Hadi Ramadani; Durroh Humairoh; Inayah Fitri
Jurnal Pengabdian Harapan Ibu (JPHI) Vol 2 No 1 (2020): Jurnal Pengabdian Harapan Ibu (JPHI)
Publisher : Sekolah Tinggi Ilmu Kesehatan Harapan Ibu Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (734.428 KB) | DOI: 10.30644/jphi.v2i1.375

Abstract

Air merupakan sumber kehidupan bagi mahluk hidup. Air merupakan kebutuhan yang paling penting dalam kehidupan manusia terutama air tawar yang bersih dan sehat. Namun demikian, kelangkaan dan kesulitan mendapatkan air bersih dan layak pakai menjadi permasalahan yang mulai muncul di banyak tempat dan semakin mendesak dari tahun ke tahun. Salah satu cara untuk mengatasi kendala tersebut dengan menjernihkan air dengan bantuan pasir, sabut air, pecahan genting, pasir, dan arang. Program ini bermaksud memberi edukasi pada siswa mengenai cara penjernihan air secara sederhana. Tujuan dari dilaksanakan program ini yaitu memberikan informasi tentang penjernihan air secara sederhana dengan menggunakan pasir, sabut air, pecahan genting, pasir, dan arang di SD Islamic International School Pesantren Sabilil Muttaqien Kediri. Metode yang digunakan dalam proses penyuluhan yang dilaksanakan di ini yaitu dengan menggunakan tiga metode yaitu: metode ceramah, tanya jawab dan demonstrasi menggunakan pasir, sabut air, pecahan genting, pasir, dan arang. Hasil yang dicapai adalah para siswa mengetahui bahaya air yang tercemar cara menjernihkan air secara sederhana. Antusias para siswa terlihat saat praktik cara menjernihkan air secara sederhana dengan menggunakan pasir, sabut air, pecahan genting, pasir, dan arang.
The Effect of Extraction Method on Total Flavonoid Content of Ageratum conyzoides Ethanol Extract Wardhani, Irma Yuniar; Ramadani, Aisyah Hadi; Widyaningrum, Fitri; Famelia, Vena
Journal Of Biology Education Vol 6, No 2 (2023): Journal Of Biology Education
Publisher : Tadris Biologi IAIN Kudus

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21043/jobe.v6i2.23398

Abstract

This research is a type of comparative research using a quantitative approach. The study population was the population of Bandotan plants in the Kudus and Jepara regions. The organ parts taken from the plant were leaves, while the stems, roots and flowers were not extracted in this study. The extraction method used maceration and soxhletation with 96% ethanol solvent. Furthermore, to determine the total flavonoid content of Ageratum conyzoides leaf extraction results using the UV Vis Spectrophotometric method, it is known that Ageratum conyzoides leaf extract using the Soxhletation method has a higher total flavonoid content (0.32% b/v) compared to the Maceration method (0.22% b/v). The results of the total flavonoid test were then analyzed using the Mann Whitney U Test. Based on the Mann Whitney U Test shows that the value of Asymp. Sig. (2- tailed) of 0.102 > 0.05. Therefore, it can be concluded that there is no significant difference between maceration and soxhletation extraction methods on total flavonoids of Ageratum conyzoides extract.
Antibacterial Activity of Pineapple Peel (Ananas comosus) Eco-enzyme Against Acne Bacterias (Staphylococcus aureus and Prapionibacterium acnes) Ramadani, Aisyah Hadi; Karima, Rizkya; Ningrum, Riska Surya
Indonesian Journal of Chemical Research Vol 9 No 3 (2022): Edition for January 2022
Publisher : Jurusan Kimia, Fakultas Sains dan Teknologi, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598//ijcr.2022.9-nin

Abstract

Acne (acne vulgaris) is a skin disease caused by infection or inflammation of the pilosebaceous unit. Staphylococcus aureus and Prapionibacterium acnes was the main actor in the infection. Eco-enzyme that is made from pineapple peel has been stated that have inhibitory activity against gram-positive and negative bacteria, also applied as home industry soap. This study objective as scientific support that Eco-enzyme phytochemical compounds had the antibacterial activity to the acne bacteria. Eco-enzyme was composed of pineapple peel, brown sugar, and water with the ratio of 3:1:10 for 3 months fermentation time. Eco-enzyme was screened of its phytochemical compound and antibacterial activity against S.aureus and P.acnes by dilution with various concentrations (1.5625%, 3, 125%, 6.25%, 12.5%, 25%, and 50% v/v) and various control. The minimum inhibition concentration (MIC) was then tested by diffusion method to determine the inhibition zone with 3 replication using the higher concentration (50%, 75%, 100% v/v). The result showed that eco-enzyme has a clear yellow colour, contains tannin and saponin, MIC of S.aureus and P.acnes bacteria is 50%. The dilution test of eco-enzyme gives the most effective concentration to inhibit S.aureus at eco-enzyme 100% (v/v), contrasted to P. acnes which didn’t show the best inhibition concentration.
Pengaruh Ekstrak Etanol Daun Serai Wangi Cymbopogon nardus (L.) Rendle Terhadap Mortalitas Wereng Coklat Niparvata lugens Stal. (Hemiptera: Delphacidae) Ramadani, Aisyah Hadi; Af'idah, Silvi Wasi'atul; Tamam, Muhammad Badrut
BIOMA: Jurnal Biologi dan Pembelajarannya Vol 5 No 2 (2023): BIOMA: Jurnal Biologi dan Pembelajarannya
Publisher : Universitas Sulawesi Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31605/bioma.v5i2.2985

Abstract

Serangan hama wereng masih menjadi permasalahan utama pada pertanian padi. Solusi pemberantasan hama dengan pestisida kimia justru menimbulkan resistensi dan ledakan populasi. Pestisida nabati dengan ekstrak tumbuhan menjadi alternatif yang dapat digunakan. Penelitian ini bertujuan untuk mengetahui berapa konsentrasi ekstrak etanol daun serai wangi Cymbopogon nardus (L.) Rendle sebagai insektisida alami yang berpengaruh efektif terhadap mortalitas wereng coklat Nilaparvata lugens Stal. Penelitian ini menggunakan Rancangan Acak Lengkap dengan enam perlakuan, yaitu kontrol negatif berupa 100ml akuades, kontrol positif menggunakan insektisida planum 100ml, konsentrasi ekstrak 2%, 3%, 4% dan 5%. Data yang diperoleh dari pengamatan, dianalisis menggunakan uji probit untuk mengetahui nilai dari LC50 dan LT50 dilanjutkan dengan uji ANOVA dan uji BNT (Beda Nyata Terkecil). Hasil analisis data oneway anova menunjukan taraf signifikansi berbeda nyata (α < 0,05) memiliki nilai 0,01, artinya ekstrak etanol daun serai wangi mempengaruhi mortalitas wereng coklat. Konsentrasi 5% merupakan konsentrasi ekstrak serai wangi paling efektif untuk membunuh wereng coklat pada tahap nimfa instar III. Hasil probit menyatakan bahwa LC50 ekstrak serai wangi dalam membunuh wereng coklat adalah 5.424% pada durasi infeksi selama LT50 3,7 jam.
Effect of Curvularia andropogonis Fungus Infection on Epidermal Tissue Anatomy of Citronella Leaves (Cymbopogon nardus (L.) Rendle) Solekha, Rofiatun; Halma, emil Milhatul Maflahah; Ramadani, Aisyah Hadi; A’in, Nur Khurotul
BEST Journal (Biology Education, Sains and Technology) Vol 6, No 2 (2023): September 2023
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v6i2.7360

Abstract

Curvularia andropogonis is a plant pathogenic fungus that causes red spot disease on citronella leaves it infects. This fungus can cause long splotches along the tips and edges of leaves which can result in damage to the entire leaf. In citronella plants, anatomical analysis is needed in the form of analysis of the value of the number and density of stomata and trichomes. Stomata are one of the structural defenses for plants against environmental stresses such as pathogenic infections. In addition to stomata, trichomes also contribute to plant defense and have a protective function. This study aims to analyze the influence of C. andropogonis fungal infection on changes in the anatomy of the epidermal tissue of citronella leaves and to determine differences in the density of stomata and trichomes in normal citronella leaves and citronella leaves infected with C. andropogonis fungus. This research method uses analysis of the number and density of stomata and trichomes using paired t test. The results showed that the average stomata density of healthy citronella leaves was ±470 stomata/mm2 and after being infected, the average density was ±312 stomata/mm2. The average density of healthy citronella leaf trichomes was ±267 trichomes/mm2 and after infection the average density was ±222 trichomes/mm2.  
Analisis Kelimpahan dan Karakteristik Mikroplastik pada Perairan Hilir Sungai Bengawan Solo Setiyowati, Putri Ayu Ika; Bukhori, Fika Nur Fitriana Putri; Ramadani, Aisyah Hadi
BEST Journal (Biology Education, Sains and Technology) Vol 7, No 1 (2024): Juni 2024
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v7i1.8632

Abstract

Mikroplastik merupakan komponen plastik dengan ukuran ( 5 mm). Banyaknya mikroplastik yang terdapat pada air di bagian hilir Sungai Bengawan Solo disebabkan oleh sampah plastik yang banyak terdapat di sepanjang badan sungai. Penelitian ini dilakukan untuk mengetahui karakteristik mikroplastik pada perairan hilir Sungai Bengawan Solo. Metode yang digunakan untuk mengidentifikasi mikroplastik dalam air mengacu pada NOAA atau National Oceanic and Atmospheric Administration. Jenis mikroplastik seperti fragmen, pelet, serat, dan film diidentifikasi di tiga lokasi pengambilan sampel hilir. Mikroplastik ini tersedia dalam berbagai warna, antara lain hitam, coklat, merah, hijau, dan biru. Selain itu, ukuran mikroplastik di air sungai Bengawan Solo terdiri dari berbagai variasi, dengan kisaran 150,00 µm hingga 478,88 µm. Kelimpahan mikroplastik pada air sungai Bengawan Solo menunjukkan nilai sig = 0,644 yang berarti 0,05 artinya tidak terdapat perbedaan yang signifikan pada ketiga lokasi pengambilan sampel. Hasil kelimpahan dan karakteristik mikroplastik sejalan dengan data parameter lingkungan di masing-masing lokasi sampling. Pada data  pengukuran parameter pH, suhu, DO, dan salinitas menunjukkan nilai p sig = 0,05 yang artinya tidak terdapat perbedaan yang signifikan antara parameter kualitas air di ketiga lokasi samplin. Temuan ini menggarisbawahi kompleksitas dampak lingkungan yang dapat timbul dari peningkatan mikroplastik di lingkungan perairan.
The Quantification of Lead Heavy Metals Levels on Mujair Fish (Oreochromis mossambicus) Organs From Brantas and Bengawan Solo River, East Java Province, Indonesia Putri Ayu Ika Setiyowati; Aisyah Hadi Ramadani; Suhariyati Suhariyati; M Ainul Mahbubillah
Al-Kauniyah: Jurnal Biologi Vol 17, No 2 (2024): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

AbstractOreochromis mossambicus (O. mossambicus) frequently found in the Brantas and Bengawan Solo rivers, Java island, Indonesia. However, heavy metals produced from anthropogenic activities can enter the water and accumulate in organisms living in the river. This study aimed to determine the lead (Pb) heavy metal in the gills, flesh, and intestines of O. mossambicus living in the two aforementioned rivers and to measure the Pb levels in each river. The results showed that the Pb in the O. mossambicus organs in the Bengawan Solo river was as follows 3.159 mg/kg in the gills; 1.930 mg/kg in the intestine; and 2.511 mg/kg in flesh, while in the Brantas river it was follows 1.600 mg/kg in gills; 1.402 mg/kg in the intestine; and 1.455 mg/kg flesh. Pb levels in each river water were 0.568 mg/mL in the Brantas river and 0.525 mg/mL in the Bengawan Solo river. Based on the data obtained, it can be concluded that the Pb content in fish organs and river water has exceeded the quality standard for Pb levels according to the government regulation No.82 2001 (SNI 7387:2009), that is, 0.3 mg/kg in organs and 0.03 mg/L in water. The results of this study are expected to be a concern for the authorities in order to revitalize the river to restore the function and support the survival of river biota.AbstrakIkan mujair (Oreochromis mossambicus) banyak ditemukan di sungai Brantas dan Bengawan Solo, namun aktivitas antropogenik yang menghasilkan logam berat dapat masuk ke perairan sehingga terakumulasi dalam organisme yang hidup di perairan tersebut. Penelitian ini bertujuan untuk mengetahui kandungan logam berat timbal (Pb) pada insang, daging, dan usus pada O. Mossambicus yang hidup di kedua sungai tersebut serta mengukur kandungan Pb pada masing-masing air sungai. Hasil penelitian menunjukkan bahwa kandungan Pb pada organ O. mossambicus di sungai Bengawan Solo adalah sebagai berikut 3.159 mg/kg pada insang; 1.930 mg/kg di usus; dan 2.511 mg/kg pada daging, sedangkan di sungai Brantas adalah sebagai berikut 1.600 mg/kg pada insang; 1,402 mg/kg pada usus; dan 1,455 mg/kg pada daging. Kadar Pb pada masing-masing air sungai adalah 0,568 mg/mL (sungai Brantas) dan 0,525 mg/mL (sungai Bengawan Solo). Berdasarkan data yang diperoleh dapat disimpulkan bahwa kandungan Pb pada organ ikan maupun air sungai sudah melebihi baku mutu kadar Pb pada organ yaitu 0,3 mg/kg (SNI 7387:2009) dan 0,03 mg/L pada perairan (PP No.82 tahun 2001). Hasil penelitian ini diharapkan dapat menjadi perhatian pihak-pihak terkait agar dapat melakukan revitalisasi sungai guna mengembalikan fungsi dan mendukung keberlangsungan hidup biota sungai.