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Analysis of individual cathecin from green tea extracted and fractionated by column chromatography Rohdiana, Dadan; Shabri, Shabri
Jurnal Penelitian Teh dan Kina Vol 15, No 2 (2012)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (347.752 KB) | DOI: 10.22302/pptk.jur.jptk.v15i2.24

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A study determination of solvent and eluent on extraction process and fractination of individual cathecin especially epigallocathecin gallate (EGCG) by column chromato­graphy method. This study devided on two steps, i.e. determination of raw material, sol­vent, process optimation for extraction and fravtination of green tea extract, evapo­ration and drying. Characterization of polyphenol conducted by visible spectro­photo­meter, analysis of individual cathecin by HPLC. This study was did in Laboratory of Processing Technology and Laboratory of Testing for Tea and Cinchona, Research Institute for Tea and Cinchona, at September 2007 up to March 2008. Result of this study shown that ratio of solvent aceton and water 7:3 v/v, ratio of tea and solvent 1:15 w/v for 15 minutes at 55-60ºC obtained 53.39% polyphenol on extract and yield gradiently produce extract with purity of cathecin is 94% b/b, EGCG 64.03% from feed with cathecin content is 82.96% w/w and EGCG 42.34% w/w.
Antidiabetic activity of of green tea ethanol extract on white mouse Rohdiana, Dadan; Firmansyah, Adang; Setiawati, Anik; Yunita, Nuni
Jurnal Penelitian Teh dan Kina Vol 15, No 1 (2012)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (288.443 KB) | DOI: 10.22302/pptk.jur.jptk.v15i1.19

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In China, India, United State, and some small other countries, tea has been consumed to prevent various disease, such as diabetes mellitus. A number of recent research revealed that consumption of green tea has an impact on the ability of insulin sensitivity in reducing glucose content in human body. Research to know antidiabetic activity of ethanol extract of green tea on experimental rats has been conducted using glucose tolerance method on rats. Results showed that application of 720 mg/kg BW of ethanol extract of green tea at 120, 180, and 240 minutes after application could reduce glucose content in the body. While applications of 180 mg/kg BW and 360 mg/kg BW of ethanol extract of green tea reduced glucose content of the body at 180 and 240 minutes after application respectively.
(1,1-Diphenyl-2-Picrylhydrazyl) free radical scavenging activity of white tea base on temperature and period of brewing Rohdiana, Dadan; Arief, Dede Zainal; Somantri, Mamay
Jurnal Penelitian Teh dan Kina Vol 16, No 1 (2013)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (332.03 KB) | DOI: 10.22302/pptk.jur.jptk.v16i1.87

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Research of 1,1-Diphenyl-2-Picrylhydrazyl (DPPH) free radical scavenging activity has been done conducted on Laboratory of Quality Assesment, Research Institute for Tea and Cinchona, and Laboratory of Chemistry, Chemistry Department, Faculty of Matematic and Natural Science, Padjdjaran University. The result showed that among four white tea from different estates, total polyphenol content on Gambung White Tea has the highest. Total polyphenol content of Gambung white tea i.e 25,52%. Brewing process on 95oC for 9 minutes obtained the highest polyphenol content on tea infusion i.e. 6,01% and the lowest EC50 i.e. 35,41 µl/ml. This research showed that presence of positive correlation between total polyphenol and antioxidant activity base on EC50. The correlation value in this research i.e. 0,9066.
UJI AKTIVITAS ANTIBAKTERI LIMA BELAS JENIS MUTU TEH HITAM ORTODOKS ROTORVANE DAN TEH PUTIH (CAMELLIA SINENSIS VAR. ASSAMICA) PADA STAPHYLOCOCCUS AUREUS ATCC 6538 Insanu, Muhamad; Maryam, Ida; Rohdiana, Dadan; Wirasutisna, Komar Ruslan
Acta Pharmaceutica Indonesia Vol 42, No 1 (2017)
Publisher : School of Pharmacy Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (951.776 KB)

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ABSTRAKCamellia sinensis (L.) Kuntze atau teh merupakan tumbuhan yang berasal dari suku theceae, digolongkan menjadi beberapa jenis, yaitu teh hitam, teh oolong, teh hijau, dan teh putih.  Secara tradisional teh digunakan sebagai obat kumur hal ini diperkuat oleh beberapa penelitian sebelumnya yang membuktikan aktivitas antibakteri terhadap bakteri yang ada pada rongga mulut antara lain Staphylococcus aureus. Penelitian ini bertujuan untuk membandingkan aktivitas antibakteri dari lima belas mutu teh hitam dan teh putih yang diekstraksi dengan pelarut yang berbeda kepolaran. Serbuk simplisia daun teh diekstraksi secara maserasi menggunakan pelarut dengan kepolaran yang meningkat, yaitu n-heksana, etil asetat, dan etanol. Ekstrak difraksinasi menggunakan ekstraksi cair-cair. Seluruh ekstrak dan fraksi diuji aktivitas antibakterinya terhadap Staphylococcus aureus ATCC6538 menggunakan metode mikrodilusi dan difusi agar. Fraksi yang paling kuat aktivitas antibakterinya diuji menggunakan biaoautografi. Hasil percobaan menunjukkan ekstrak etanol teh putih memiliki aktivitas antibakteri yang paling kuat dengan nilai Konsentrasi Hambat Minimum (KHM) 78,13 μg/mL, sedangkan berbagai macam ekstrak teh hitam KHM-nya bervariasi antara 625-2500 μg/mL. Fraksi etil asetat dari ekstrak etanol teh putih memiliki aktivitas paling kuat dengan nilai KHM 156,25 μg/mL. Hasil bioautografi fraksi etil asetat menunjukkan hambatan pertumbuhan bakteri S. aureus pada nilai Rf 0,5 dan 0,76. Berdasarkan reaksi warna kedua nilai Rf ini termasuk golongan  flavonoid.Kata kunci: antibakteri, Staphylococcus aureus, teh putih, teh hitam orthodoxEVALUATION ON ANTIBACTERIAL ACTIVITY OF FIFTEEN DIFFERENT CLASSES OF ORTHODOX ROTORVANE BLACK TEA AND WHITE TEA (CAMELLIA SINENSIS VAR. ASSAMICA) AGAINST STAPHYLOCOCCUS AUREUS ATCC 6538ABSTRACTCamellia sinensis (L.) Kuntze or tea is a plant belonging to Theaceae family. It can be classified into four different classes, which are black tea, oolong tea, green tea and white tea. Traditionally, tea was used as a mouthwash. It was strengthened by previous researches; tea had antibacterial activity especially against bacteria that live in the oral cavity such as Staphylococcus aureus. The aim of this study was to evaluate the antibacterial activity of 15 different classes of black tea and white tea which were extracted by various organic solvents. The crude drugs were obtained by maceration using three different solvents, which were n-hexane, ethyl acetate, and ethanol. The extract was fractionated by liquid-liquid extraction. All extracts and fractions were evaluated their antibacterial activity using microdilution and disc diffusion. The strongest antibacterial fraction was continued to bioautography assay. Based on the result the ethanol extract of white tea showed the strongest activity with the Minimum Inhibition Concentration (MIC) was 78.13 μg/mL while the other extracts of black tea were ranged between 625-2500 μg/mL. Ethyl acetate fraction of white tea ethanol extract had MIC value which was 156.25 μg/mL.  Bioautography showed the rf values of 0.5 and 0.76 inhibited the growth of bacteria. Based on spot test, they were flavonoid compounds.Keywords: antibacterial, Staphylococcus aureus, white tea, orthodox black tea
PEMBUATAN SABUN PADAT TRANSPARAN MENGGUNAKAN MINYAK KELAPA SAWIT (Palm oil) DENGAN PENAMBAHAN BAHAN AKTIF EKSTRAK TEH PUTIH (Camellia sinensis) Widyasanti, Asri; Farddani, Chintya Listiarsi; Rohdiana, Dadan
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol 5, No 3 (2016)
Publisher : The University of Lampung

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Transparent soap is a kind of solid soap. The addition of white tea extracts could be expected to increase theadvantageof transparent soap. The purpose of this research was to determine process and formulation for themaking of transparent solid soap using palm oil based with addition white tea extracts and its effect to thecharacterictics of transparent solid soap. This reasearch was used laboratory experimental method using descriptiveanalysis. The treatment of this reasearch were addition of white tea extracts using concentrations 1% (w/v) withthe addition of A= 0% (w/v), B=0.5% (w/v), C=1.0% (w/v), and D=1.5% (w/v). Parameters observed wereorganoleptic, chemical properties, antibacterial activity, hardness, and stability of foam. Organoleptic test resultsshowed that panelists prefer soap treatment B with a percentage of 36.67%. The chemical properties of this soapwas in accordance with SNI solid soap No.06-3532-1994, except amount of fatty acids. The value of water contentand evaporated substance was 12.17%, the content of free alkali was 0.101%, the content of unsaponifiedfraction was 2.10%, and the amount of fatty acid was 35.67%. pH value was in accordance ASTM D 1172-95 withpH value of 10. The value of hardness was 0.0091 mm/g/s, stability of foam was 39.08%, and antibacterialactivity with inhibition zone diameter was 11.28 mm. It proved that making transparent solid soap using a palmoil based and the addition of white tea extract as active ingredients could be applied by the community.Keywords:Transparant Solid Soap, Palm Oil, White Tea Extract
Delayed diagnosis of nasopharyngeal carcinoma in a patient with early signs of unilateral ear disorder Adham, Marlinda; Rohdiana, Dadan; Mayangsari, Ika D.; Musa, Zanil
Medical Journal of Indonesia Vol 23, No 1 (2014): February
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (299.973 KB) | DOI: 10.13181/mji.v23i1.689

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Nasopharyngeal carcinoma (NPC) is the most frequent head and neck malignancy in Indonesia. Misdiagnosis of NPC is common because of unspecific symptoms as unilateral ear complaint. This case reminds doctors of the early symptoms of NPC and of other factors which lead to misdiagnosis and addresses also patients and their families. Reported is a 44 years old man with unilateral ear disorder that had been treated by otorhinolaryngologists, an ophthalmologist, a neurologist, and dentist first, but diagnosed with nasopharyngeal carcinoma stage IVA (T4N1M0) one year later. NPC has unspecific early symptoms such as unilateral ear disorder. Primarily doctors, but also patients and their families should be aware of unilateral ear complaint.Keywords: misdiagnosis, nasopharyngeal carcinoma, unilateral ear disorders
DIVERSIFIKASI PRODUK BERBASIS TEH PADA INDUSTRI PANGAN, FARMASI, DAN KOSMETIK Diversification of Tea Based Products in the Food, Pharmaceutical and Cosmetic Industry Prawira-Atmaja, Mukhammad Iqbal; Rohdiana, Dadan
Perspektif Vol 17, No 2 (2018): Desember 2018
Publisher : Puslitbang Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (822.38 KB) | DOI: 10.21082/psp.v17n2.2018.150-165

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ABSTRAKTeh adalah minuman populer di dunia yang memiliki manfaat kesehatan. Selama periode 2006-2016 jumlah produksi dan konsumsi teh dunia meningkat signifikan. Intenational Tea Committee melaporkan bahwa jumlah produksi teh dunia di tahun 2016 mencapai 5,46 Juta Ton dengan jumlah konsumsi rata-rata mencapai 5,1 juta Ton. Namun, peningkatan produksi dan konsumsi teh tidak berbanding lurus dengan harga jual teh dipasaran. Rerata harga teh di rumah lelang berkisar US$ 2,6/kg.Indonesia merupakan negara produsen teh ke 7 dengan sumbangsih 3% dari total ekspor dunia. Tahun 2016 produksi teh Indonesia mencapai 125.000 Ton menurun 7% jika dibandingkan dengan tahun sebelumnya. Penurunan jumlah produksi teh tersebut salah satunya dikarenakan luas area tanaman teh dalam beberapa tahun terus mengalami penurunan dan banyak beralih fungsi menjadi tanaman hortikultura. Selain itu biaya produksi seperti upah buruh, pengelolaan lahan, dan utilitas yang terus meningkat berdampak terhadap semakin sedikit margin keuntungan yang diperoleh. Perkembangan dan terobosan teknologi dewasa ini telah banyak berperan di dalam mengembangkan keragaman produk berbasis teh. Diversifikasi produk berbasis teh mampu meningkatkan nilai tambah dan nilai jual produk tersebut. Keragaman produk berbasis teh telah banyak diaplikasikan dan dimanfaatkan pada industri pangan, industri farmasi, maupun industri kosmetik kecantikan. Meskipun begitu, kestabilan senyawa bioaktif teh seperti polifenol, katekin, theaflavin, dan senyawa bioaktif lainnya masih menjadi isu utama. Masih harus dilakukan penelitian lebih lanjut untuk mengetahui kestabilan senyawa bioaktif teh pada saat pengolahan, pengemasan dan penyimpanan, dan selama distribusi hingga ke tangan konsumen. Pemanfaatan bagian tanaman teh yang lain seperti biji dan bunga teh perlu dilakukan penelitian lebih lanjut. Pemanfaatan tersebut diharapkan bisa mendukung pohon industri teh dan mampu meningkatkan keragaman produk berbasis teh di masa mendatang. Review ini merangkumkan diversifikasi produk berbasis teh yang telah banyak dikomersialkan di pasaran dan memiliki nilai jual yang tinggi.ABSTRACTTea is a popular beverage in the world that have health benefits. Over the period 2006-2016 the amount of tea production and consumption in the world increased significantly. International Tea Committee reported that amount of world tea production in 2016 reached 5.46 million tons with total consumption reaching 5. 1 million tons. However, the increase in tea production and consumption is not directly proportional to the selling price of tea in the market. The average price of tea at the auction house is around US $ 2.6 / kg. Indonesia is the 7th tea producing country with a contribution of 3% of total world exports. In 2016 Indonesian tea production reached 125,000 tons, down 7% compared to the previous year. Decrease the amount of tea production due to a decline in the area of tea and many converted into horticultural crops. The condition is worsen by the increasing cost of processing production in the plantation and in the factory so that the profit margin obtained is very low. Recently developments and technological breakthroughs have much role in developing diversified tea products so as to increase the selling tea products. The diversity of tea-based products have been widely applied in the food industry, the pharmaceutical industry, as well as beauty cosmetic industries. Nevertheless, the stability of bioactive compounds such as tea polyphenols, catechins, theaflavins, and other bioactive compounds still a major issue. Further research is still needed to determine the stability of tea bioactive compounds during processing, packaging and storage, and during distribution to consumers. Utilization of other plant parts such as seed tea and flower tea needs to be done further research. The utilization is expected to support the tea industry tree and be able to increase the diversity of tea-based products in the future. This review we will summarize the diversification of tea-based products that have been widely commercialized on the market and have high selling points
Aktivitas Antioksidan Ekstrak Teh Putih dengan Metode DPPH Widyasanti, Asri; Rohdiana, Dadan; Ekatama, Novriana
EDUFORTECH Vol 1, No 1
Publisher : Program Studi Pendidikan Teknologi Agroindustri, UPI (Universitas Pendidikan Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17509/edufortech.v1i1.3966

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Teh putih merupakan jenis teh dengan proses pengolahan yang minim (pelayuan dan pengeringan) diduga menyebabkan kandungan polifenol dan aktivitas antioksidan lebih tinggi dibandingkan dengan jenis teh lainnya. Tujuan penelitian ini adalah mengetahui aktivitas antioksidan ekstrak teh putih dengan menggunakan metode DPPH (2,2 Difenil -1- Pikrilhidrazil) dan korelasinya dengan kadar polifenol. Metode ekstraksi yang digunakan adalah maserasi bertingkat dengan menggunakan pelarut n-heksana, etil asetat dan etanol 96%.Metode penelitian yang digunakan adalah metode eksperimental laboratorium dengan menggunakan analisis deskriptif.Ekstrak teh putih dibuat dengan menggunakan pelarut n-heksana, etil asetat dan etanol 96%. Parameter yang diamati adalah nilai IC50 dan kandungan total polifenol beserta korelasinya. Aktivitas antioksidan tersebut diwakilkan dengan nilai IC50 atau konsentrasi yang dapat meredam 50% radikal bebas. Nilai IC50 dari ekstrak n-heksana, etil asetat dan etanol 96% berturut-turut adalah 203,7846 ppm; 11,207 ppm dan 5,153 ppm. Kontrol positif aktivitas antioksidan menggunakan vitamin C dengan nilai IC50 sebesar 6,285 ppm. Kadar polifenol dari ekstrak teh putih dengan pelarut n-heksana, etil asetat dan etanol 96% berturut-turut adalah 22,01 %; 57,54% dan 59,32%. Nilai korelasi dari aktivitas antioksidan (IC50) dan kadar polifenol 0,99 
AKTIVITAS PENGANGKAPAN RADIKAL POLIFENOL DALAM DAUN TEH Rohdiana, Dadan
Indonesian Journal of Pharmacy Vol 12 No 1, 2001
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (127.731 KB) | DOI: 10.14499/indonesianjpharm0iss0pp52-58

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Ability of free radical scavengers of various tea extracts (black tea, fermented tea; green tea , unfermented tea; oolong dan pouchong tea semifermented tea) was studied. The scavenging effects on active oxygen decreased in order semifemented tea > unferfmnted tea > fennented lea. Antioxidant activity on tea often might related wthi catechin presence. Catechin activity as antioxidant estabilished. Several epidemilogical and phartnacokgical research shown that catechin had a strongly antioxidan activity. (-)-epigallocatechin gallate (EGCG) and (-)-epicatechin gallate (ECG) are the major catechin of ~ tea This compounds had a strongly scavenging activity on free radical such as superoxide, hydrogen superoxide, DPPH and peroxynitdte. This review aim to exploited~ tea Oyphenols ability as free radicals sw vengers Key word: antioxidant activity, active oxygen, scavenging effect
FORMULASI TEH CAMELIA-MURBEI DENGAN BUBUK JAHE (Zingiber officinale) DAN ASAM JAWA (Tamarindus indica, L.) SEBAGAI MINUMAN KESEHATAN UNTUK MENINGKATKAN RESPON IMUN TIKUS Sri Darningsih; Clara Meliyanti Kusharto; Sri Anna Marliyati; Dadan Rohdiana
Jurnal Gizi dan Pangan Vol. 3 No. 2 (2008)
Publisher : Food and Nutrition Society of Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (141.806 KB) | DOI: 10.25182/jgp.2008.3.2.61-70

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The Camelia-mulberry tea has a big potential to becomes a healthy beverage according to its bioactive components, and added by red ginger powder and tamarind. Those ingredients may increase immunity againts various diseases. The aims of this study was to develop formula of a healthy beverage contained of two varieties of leaves (camelia and mulberry), with Ginger (Zingiber officinale) and Tamarind (Tamarindus indica, L.)   This main research  used mice strain Spraque Dawley, 1.5-2 month old with  weight 75–105 gram. The instruments used in this study were tea maker, organoleptic test and ELISA test. The experimental design used factorial complete random design with 5 groups, where on each group consists of 5 mouse, with two selected variables of the best camelia-mulberry tea, i.e., multikaulis enzymatic oxidation + Gambung 7 enzimatic non-oxidation; bulk tea (Tea A) with difference time observation ( 0, 2 and 4 weeks).  The given dosage was 1 ml/100 gram mice weight, with different concentration of camelia-mulberry tea i.e. 3, 6, 9, and 12 gram/ml. The statistical analysis of variance (ANOVA) shows that a significant different exist for the time observation of a mice serum IgG (p<0.05).  Followed by Tukey test, which  shows that a significant differences exist on IgG average at the beginning and after  the 2nd weeks of treatment, and the 2nd weeks vs 4th weeks treatment.
Co-Authors A Rustamsyah A Rustamsyah, A Achmad Imron Rosyadi Adang Firmansyah Akhmad Endang Zainal Hasan Amalia Rahma Ananda, Marsha Radhia Andita Mega Priantiwi Andita Mega Priantiwi, Andita Mega Andri Agustav Wirabudi Angga Rusdinar Anik Setiawati Anik Setiawati, Anik Ardi Rustamsyah Ardi Rustamsyah Arista Budiman Arista Budiman, Arista Asep W. Perdana Asri Widyasanti Awaliah, Nisa Azmi, M. Haikal Beauty Azhary Bina Lohita Sari Budi Setiawan Cece Sumantri Citra Ratu Pelangi Citra Ratu Pelangi, Citra Ratu Clara Meliyanti Kusharto Darningsih, Sri Dede Zainal Arief, Dede Zainal DIMAS ANDRIANTO Dinda Nuraini Maulfida Dini Febrianty Dong Ho Lee Dwi Putra, Januar Hafiz Dwiyan Nugraha Dytha Andri Deswati, Dytha Andri Ekatama, Novriana Evy Damayanthi Faillah, Umi Lutfiatul Fani Fauziah Farddani, Chintya Listiarsi Fauzan, Fajar Akmal Fauzi, Ridho Hadi Riyadi Hanipah, Anida Hanny Hafiar Hasna Zakiyah Heddy Julistiono Heri Aryadi Hidayat, Taufik Septiyan Hijaya, Andi Hilman Maulana Hilman Maulana Hilman Maulana I Gusti Bagus Wiksuana Ida Maryam Ika D. Mayangsari Ikrawan, Yusep Isti Agnia Lutfiah Isti Agnia Lutfiah, Isti Agnia Komar Ruslan Wirasutisna Komar Ruslan Wirasutisna Kristin, Julia M Iqbal Prawira-Atmaja M. Iqbal Prawira-Atmaja Mamay Somantri Mamay Somantri, Mamay Marlinda Adham Martini, Rina Maryam, Ida Mimin Kusmiyati, Mimin Miranti Putri Utami Muhamad Alvin Adriyan Muhamad Insanu Muhammad Insanu Muhammad Iqbal Prawira-Atmaja Mukhammad Iqbal Prawira-Atmaja Mukhammad Iqbal Prawira-Atmaja Murdijati Gardjito Novi Susanti Nuni Yunita Nuni Yunita, Nuni Nurlita, Selvi Nurzanah, Fitri Prawira-Atmaja, Mukhammad Iqbal Rachmat Mauludin Rahayu, Dien Puji Rendy Munadi Rimbawan , Rimbawan Rimbawan Rina Martini Rohayati Suprihatini Rosialani, Resa Rosyanne Kushargina Rosyanne Kushargina Sahara, Putri Siti Sari Agustin Shabri Shabri Shabri Shabri Shabri Shabri Shabri Shabri Shabri Shabri, Shabri Siti Hajar Siti Hajar Sri Anna Marliyati Sri Raharjo Sugeng Harianto Sugeng Harianto SUGENG HARIANTO Sugeng Haryanto SUHARYANTO SUHARYANTO Syumillah Saepudin Tri Halimah Tri Panji Yayat Sudaryat Yunita Al-Azzahra Yusianto Yusianto Yusianto Yusianto, Yusianto Zahra, Nur Adni Azkia Zanil Musa