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Isolation and Characterization of Cellulolytic Bacteria from Bonoloyo, Cemetery Risnasari, Wanda Datik; Tyastuti, Erma Musbita; Sidiq, Yasir; Rahayu, Triastuti
Proceeding of International Conference on Biology Education, Natural Science, and Technology 2023: Proceeding of International Conference on Biology Education, Natural Science, and Technology
Publisher : Universitas Muhammadiyah Surakarta

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Abstract

A public cemetery (TPU) is land provided by the government to bury bodies. The culture of funerals in Indonesia is varied but generally involves being buried in the soil with wrapped clothes, covered wooden boards, and tombs. The material contains cellulose that will be broken down by cellulolytic microorganisms, including bacteria. The exploration of the cellulolytic bacteria of TPU in Indonesia has not been carried out, so this study aims to investigate and identify cellulose bacteria isolates from TPU. This research is a non-experimental study for the isolation of bacteria in the soil of TPU Bonoloyo, Surakarta. A selection of cellulite bacteria is grown in CMC media. Isolates that show cellulolytic activity are identified by observing colonial morphology and Gram coloring. Selection results of cellulolytic bacteria obtained isolates with cellulolytic index (IS) including a high category of 1 isolate (B14), a medium category of 3 isolates (B16, B27, and B45), and 9 low category isolates. The study concluded that 28.8 % showed cellulolytic activity with irregular morphological forms of white dyeing on flat surfaces with flat edges and dominated by gram-negative coccyx-shaped.
Isolation and Identification of Soil Bacteria in Pracimaloyo Public Cemetery, Kartasura Adityaradja, Bagas; Tyastuti, Erma Musbita; Sidiq, Yasir; Rahayu, Triastuti
Proceeding of International Conference on Biology Education, Natural Science, and Technology 2023: Proceeding of International Conference on Biology Education, Natural Science, and Technology
Publisher : Universitas Muhammadiyah Surakarta

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Abstract

In The Pracimaloyo Public Cemetery, the body’s decomposition occurs continuously, producing soil nutrients and minerals that affect the growth of bacteria. Bacterial population data from the cemetery area is still very limited, whereas cemeteries hold the risk of contamination with pathogenic bacteria. This research aims to find out how the population and diversity of bacteria in the Pracimaloyo Public Cemetery. Soil samples were taken from the Pracimaloyo cemetery at 2 locations (blocks 8 and 18), each at 20 and 50 cm depth. Soil samples are inoculated in Nutrient Agar media using the spread plate method. After 48 hours, colony counting, colony morphology, and gram staining observations were carried out. The rate of soil bacterial populations in blocks 8 and 18 at a depth of 20 cm was 4.23 × 107 CFU/g and 9.79 × 107 CFU/g, while at depths of 50 cm, it was 1.94 × 107 CFU/g and 1.92 × 107 CFU/g. The morphology of the bacterial colonies is dominated by circular shape, entire margin, flat elevation, and white color. 20 isolates are gram-negative and 16 isolates are gram-positive, the cell form is dominated by the bacillus.
Screening and Identification of Cellulolytic Bacteria from Public Cemetery Soil Syarifah, Siti Nur; Rahayu, Triastuti; Tyastuti, Erma Musbita; Sidiq, Yasir
BIOEDUSCIENCE Vol 7 No 2 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/11745

Abstract

Background: A public cemetery (TPU) is where bodies are buried. Before being buried, the body is wrapped in a shroud or full clothes and sometimes put in a coffin. These materials contain cellulose, a substrate for cellulolytic microorganisms, including bacteria, to decompose. Exploration of cellulolytic bacteria in TPU has not been found, even though TPU holds potential cellulolytic bodies. This study aims to determine the cellulolytic potential and identify bacterial isolates from TPU. Methods: 36 bacterial isolates tested for cellulolytic potential have been isolated from TPU Pracimaloyo Kartasura, Sukoharjo. Selection of cellulolytic bacteria used selective Carboxymethyl Cellulose (CMC) media dripped with Congo red 0.1%, while identification was based on colony morphology and Gram staining. Results: This study obtained one isolate (2.78%) showing high cellulolytic activity with a cellulolytic index (IS) 7, namely P14, 24 isolates (66.67%) in the "moderate" category, four isolates (11.11%) with category "low," and seven isolates (19.44%) did not show cellulolytic ability. Isolates cellulolytic positive have a shiny white colony color, entire edges, raised elevation, and belong to the Gram-negative coccus form. Conclusions: From the results of this study, it can be concluded that public burial sites (TPU) harbor potential cellulolytic bacteria.
Test of Hypersensitivity and Antagonistic Reaction of Endophytic Bacteria from Klutuk Banana (Musa balbisiana) Dewianty, Ratih; Rahayu, Triastuti; Sidiq, Yasir
BIOEDUSCIENCE Vol 7 No 2 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/11795

Abstract

Background: Plants’ response and antagonistic reaction against disease-causing organisms are two crucial characteristics of Plant Growth Promoting Bacteria (PGPB). Seventeen endophytic bacteria have been isolated from Musa balbisiana. However, the plants’ reaction against these isolates and their antagonistic activities against disease-causing organisms remained unknown. This study aims to (1) determine the sensitivity effect of endophytic bacteria isolates to tobacco and (2) test the antagonism of endophytic bacteria isolates against the blast disease actor, namely Pyricularia oryzae. Method: Sensitivity tests were performed by inoculating bacterial isolates into tobacco leaves with infiltration. Bacterial isolates were prepared with a minimum OD 600: 0.5. Then, a milliliter volume of each isolate was infiltrated into tobacco leaves from the abaxial side using a 3 mL syringe. The lesion on the tobacco leaves was observed seven days after inoculation (DAI). The antagonism test was carried out by growing Pyricularia oryzae on a PDA plate for seven days, subsequently transferred to an NA medium for three days then inoculated with endophytic bacteria. The assessed parameter of the antagonistic test was the formation of an inhibitory zone between endophytic bacteria and Pyricularia oryzae at 4 DAI. Results: No hypersensitive reaction of tobacco leaves against K117, K324, K38, K86, K18, K28, K102 isolates inoculation at 7 DAI. Furthermore, the antagonistic test indicated that all isolates inhibited the growth of Pyricularia oryzae, with the range of inhibition from 32.36 to 40.46%. Implication: Thus, these results revealed the PGPB characteristics in the newly isolated endophytic bacteria from the banana.
Skrining Bakteri Lipolitik dari Tanah Pemakaman Mandiri, Listy Hasti; Rahayu, Triastuti; Tyastuti, Erma Musbita; Sidiq, Yasir
BIOEDUSCIENCE Vol 7 No 3 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/12686

Abstract

Background: The cemetery is an area for the burial of corpses so that active decomposition of bodies occurs. One of the decomposition bacteria that might be found is lipolytic bacteria because the human body comprises 21,33-32,51 % lipids. Explorations of lipolytic bacteria from cemeteries in Indonesia have not been found, so this research needs to be done. This non-experimental study aimed to select and identify lipolytic bacterial isolates from the Pracimaloyo cemetery, Surakarta. Method: A total of 36 bacterial isolates were selected for their lipolytic activity using tributyrin media. The lipolytic index determines lipolytic activity. Identification of lipolytic bacteria based on colonial morphology and Gram staining. Result: The study showed that 22.2% (8 isolates) showed a lipolytic positive, with the largest lipolytic index (LI) value of 2.5 (isolate P36). Bacterial colonies are circular-shaped, have entire edges, flat elevation, and are yellow or white. Gram staining results showed that the isolated is a group of Gram-negative bacteria in the form of coccus. Conclusion: The conclusion of this study suggests that bacterial isolates from Pracimaloyo cemetery that have potential lipolytic activity, which is likely to be from the genera Pseudomonas and Klebsiella, were obtained.
PENGEMBANGAN SENTRA BUDIDAYA MELON DI PANTAI BOCOR KABUPATEN KEBUMEN MELALUI IMPLEMENTASI EDUCATION FOR SUSTAINABLE DEVELOPMENT Daryono, Budi Setiadi; Purnomo, Purnomo; Sidiq, Yasir; Maryanto, Sigit Dwi
Bioeksperimen: Jurnal Penelitian Biologi Vol 2, No 1: March 2016
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v2i1.1580

Abstract

Pesisir Pantai Bocor yang terletak di selatan Kabupaten Kebumen, merupakan salah satu lokasi yang berpotensi sebagai lahan pertanian. Sepanjang Pantai Bocor terbentang gumuk pasir yang berfungsi sebagai penghalang angin dan gelombang. Wilayah tersebut dimanfaatkan sebagai lokasi menanam melon yang menjadi komoditas unggulan daerah. Oleh sebab itu dikembangkan melon ramah lingkungan untuk menjaga kelestarian gumuk pasir. Upaya pengembangan melon ramah lingkungan termasuk dalam Education for Sustainable Development (ESD).  yang didanai UGM. Pogram ini bertujuan untuk pengembangan masyarakat Desa Setrojenar, Kabupaten Kebumen berbasis ESD. Kegiatan program ini antara lain sosialisasi tentang pelestarian gumuk pasir, pengenalan alternatif tanaman pangan yaitu umbi-umbian, penanaman tanaman Casuarina equisetifolia Linn. sebagai barier, dan pendampingan budidaya melon menggunakan potensi lokal oleh petani. Hasil kegiatan antara lain partisipasi masyarakat tinggi, gumuk pasir tidak rusak, tanaman barier dapat tumbuh baik dan petani dapat panen melon dengan kualitas baik. Kegiatan ini diharapkan dapat menjadi role model bagi masyarakat untuk terus mengembangkan budidaya melon dan tetap menjaga kelestarian gumuk pasir.
Efficacy of Endophytic Bacteria Pantoea dispersa Strain DSM 30073 on The Growth of Ipomoea reptans Rahayu, Triastuti; Sidiq, Yasir; Anhari, Minhatul Ulya; Zulperi, Dzarifah
Bioeksperimen: Jurnal Penelitian Biologi Vol 10, No 2: September 2024
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v10i2.23183

Abstract

Pantoea dispersa strain DSM 30073 is an endophytic bacterium isolated from the Ambon banana plant. From in vitro tests, this strain shows characteristics such as PGPB (Plant Growth Promoting Bacteria) and can support the growth of Cavendish banana plants, but its effect on Ipomoea reptans is unknown. The purpose of this study was to determine the efficacy of endophytic bacteria Pantoea dispersa strain DSM 30073 against the growth of Ipomoea reptans. The research design used was a one-factor Complete Randomized Design (CRD), namely inoculation frequency (P): control (P0), one-time inoculation (P1), and two-time inoculation (P2). The inoculum was confirmed to be pure by gram staining and cultured on NB medium with a 24-hour shake at room temperature. After 24 hours, the cells were harvested, and a suspension was made by adding Ringer's solution until it reached an OD600 nm of 0.8 – 1. Seeds were sterilized using 70 % alcohol followed by 1 % NaOCl for 1 minute each, then washed using sterile aquadest 3 times.  Sterile seeds were inoculated with an inoculum suspension for 30 minutes (P1). After inoculation, seeds are planted on a sterile soil medium. Repeated inoculation is carried out on the 11th day (P2). The growth of Ipomoea reptans is observed: plant height (cm), number of leaves (strands), leaf stretch (cm), wet weight of the plant (g), root length (cm), and number of roots (total). After 30 days, data on plant height 16.1 cm (control) – 17.4 cm (inoculation), wet weight of plants 1.1 g (control) – 1.4 g (inoculation 2 times), number of leaves 7 strands (control) – 8 strands (inoculation 2 times), and leaf stretch 10.2 cm – 10.9 cm (inoculation 2 times). Inoculation of Pantoea dispersa strain DSM 30073 had no effect on plant height growth, wet weight, leaf number, and leaf stretch but was significantly different in root parameters. It can be concluded that the Pantoea dispersa was not seen to support the growth of Ipomoea reptans significantly until 30 days after inoculation.
BIODEGRADASI PLASTIK OLEH CAMPURAN BAKTERI LIPOLITIK DI DALAM KOLOM WINOGRADSKY Rahayu, Triastuti; Sidiq, Yasir; Harismah, Kun; Santhyami, Santhyami; Agustina, Putri; Haryanto, Haryanto; Tyastuti, Erma Musbita; Aurelia, Fanisha; Sabdina, Berlian Achya Putri
Berita Biologi Vol 23 No 3 (2024): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2024.5706

Abstract

Plastik menjadi masalah serius karena sangat lambat didegradasi. Oleh karena itu banyak usaha dilakukan untuk mempercepat degradasinya tetapi belum menunjukkan hasil yang signifikan. Salah satu upaya yang dicoba adalah menggunakan bakteri lipolitik. Tujuan penelitian ini adalah menguji kemampuan degradasi plastik menggunakan campuran bakteri lipolitik dalam kolom Winogradsky. Penelitian ini merupakan penelitian eksperimen dengan 2 faktor perlakuan. Faktor 1; jenis plastik (P1/kresek hitam putih) dan (P2 /bungkus mie instan). Faktor 2; waktu inkubasi (W1/1,5 bulan) dan (W2/3 bulan). Plastik diuji degradasi menggunakan campuran bakteri B9 dan B28 dalam kolom Winogradsky. Data yang dianalisis berupa uji antagonisme dan identifikasi bakteri, persen pengurangan berat kering, SEM, dan FTIR. Kedua isolat tidak menunjukkan antagonisme. Isolat B9 teridentifikasi Staphylococcus saprophyticus, sedangkan B28 adalah Bacillus amyloliquifaciens. Persen pengurangan berat kering P1 lebih besar dibanding P2 sebesar 5,11 (4,91%) dan 4,99 (3,99%) masing-masing untuk 1,5 dan 3 bulan. Permukaan P2 terbentuk kerutan lebih banyak dan terdapat cekungan yang dalam di beberapa titik, sedangkan P1 masih tampak lebih halus. Hasil analisis FTIR P2 menunjukkan  munculnya spektra senyawa amina, alkane dan CO2. Campuran bakteri Staphylococcus saprophyticus dan Bacillus amyloliquifaciens mempunyai kemampuan mendegradasi plastik dalam kolom Winogradsky dan plastik 2 menunjukkan tingkat degradasi lebih lanjut dibandingkan plastik 1.
Growth of Endophytic Bacteria from Klutuk Banana Plant (Musa balbisiana Colla) with Inoculation Trial on Rice Plants (Oryza sativa) Anggun Dwi Nur Annisa; Triastuti Rahayu; Yasir Sidiq
Quagga: Jurnal Pendidikan dan Biologi Vol 16 No 1 (2024): QUAGGA : Jurnal Pendidikan dan Biologi
Publisher : Program Studi Pendidikan Biologi, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Kuningan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25134/quagga.v16i1.53

Abstract

Four potential bacteria have been isolated from Klutuk banana plant. They exhibited IAA production. Since the bacterial inoculation to plant should be performed in exponential phase of bacterial growth, information of the growth is crucial. This study aimed to analyze the growth rate of eight isolates and examine the effect of bacterial inoculation by initial trial on rice plants. For this purpose, four endophytic bacteria from Klutuk banana were cultured and examined using 600nm spectrophotometer. The growth of the isolates was calculated every two hours for 16 hours bacterial growth in the nutrient broth medium. The inoculation trial of bacteria to rice plants was conducted with three replicates. This inoculation was begun by germinating the rice seed and the radicle was observed. Then, the germinated rice seedlings were soaked in the bacterial suspension for 5 minutes in room temperature. The results showed that the exponential phase of all isolates was observed 6 hours after shaking. Inoculation using 6-hour-incubated K7 isolate significantly improved the length of root of rice plants. Additionally, this isolate improved the number of root and shoot length without significant difference. Thus, these results provide important information of the growth phase and inoculation effect new isolated endophytic and rhizosphere bacteria.
Selection of Proteolytic Bacteria from Bonoloyo Public Burial Place (PBP), Banjarsari, Surakarta, Central Java Putri Salwa Salsabilla; Yasir Sidiq; Erma Musbita Tyastuti; Triastuti Rahayu
Quagga: Jurnal Pendidikan dan Biologi Vol 16 No 1 (2024): QUAGGA : Jurnal Pendidikan dan Biologi
Publisher : Program Studi Pendidikan Biologi, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Kuningan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25134/quagga.v16i1.57

Abstract

Public Burial Place (PBP) is a cemetery for everyone. In PBP, protein decomposition occurs actively because the human body is made up of 16.4% protein, so it is most likely that proteolytic bacteria can be found. Previous research has isolated as many as 45 isolates from PBP Bonoloyo, Surakarta, but has not selected their proteolytic capabilities. The aim of the study was to isolate proteolytic bacteria from the Bonoloyo Public Cemetery and identify them. The bacterial isolate of PBP Bonoloyo is a collection from the FKIP Biological Laboratory of Muhammadiyah University of Surakarta that selected its proteolytic capabilities using the milk agar scheme (SMA). The parameters measured are the diameter of the colony and the lymphatic zone formed to obtain the value of the proteolytic index (IP). For bacterial isolates showing a positive proteolytic activity, simple identification is carried out through the observation of colonial morphology and Gramme colouring. The results of the study showed that as many as 28 isolates (62.2%) were positive for proteolytic activity, with IP values >2 for B1 and B3. Bacterial proteolytic isolates are dominantly white to yellow with irregular, filamentary, spindle, and circular colonial forms. The results of Gramme colouring show that bacterial isolates that have proteolytic abilities are a group of Gramme negative bacteria that are basil shaped. The bacterial isolate that shows the highest IP value is the B1 isolate, which is suspected to be of the genus Bacillus. This research obtained data showing that in the public cemetery (PBP), Bonoloyo stores potential as a proteolytic bacterial habitat for a group of Gramme negative bacteria.
Co-Authors 'Azmi, Abidah Ulul A.A. Ketut Agung Cahyawan W Adilla Nur Azizah Adilla Nur Azizah Adityaradja, Bagas Alanindra Saputra Alanindra Saputra Alanindra Saputra Alanindra Saputra Alanindra Saputra Anatri Desstya Anggita Juniar Laspartriana Anggun Dwi Nur Annisa Anhari, Minhatul Ulya Anisa, Rizka Sakinatun Anjani, Nofa Annur Indra Kusumadani Apriliana, Cindi Dila ARI SUSILOWATI Ariyanti, Olivia Puja Arum Dyah Ripdiyanti Astuti, Dyah Auladiyah, Alfia Sa'adul Aurelia, Fanisha Azizah, Adilla Nur Bambang Harmanto, Bambang Bambang Sumardjoko Baskoro Adi Prayitno Baskoro Adi Prayitno Bowo Sugiharto Bowo Sugiharto Bowo Sugiyarto BUDI SETIADI DARYONO Condro Nur Alim D Djumadi Daryono, Budi S Dedi Gunawan Defina Anggita Silviani Devary Pradana Setyabudi Dewianty, Ratih Dicky Eka Prasetya Dito, Bahana Safiria Donny Widianto Donny Widianto Dyah Astuti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Evi Dwi Kartikasari Fadilah, Fari Fari Fadilah Febrianti, Nisa Febriyanti, Vika Gamal Abdul Nasir Zakaria Ganno Tribuana Aji Guntur Nurcahyanto Haq, Muhammad Izzul Hardianto, Alden Ganendra Madhava Priya Hariyatmi Hariyatmi Hartanto, Nadhifah Riski Harun Joko Prayitno Haryanto Haryanto Heri Siswanto Hernawan Sulistyanto Ihsan Nugroho Irfadhila, Dwega Izzatul Azmi Mulya Jakia, Nikmatu Jamil, Nazia Jumadi Jumadi Junita Dwi Wardhani Kistantia Elok Mumpuni Kistantia Elok Mumpuni Kun Harismah Listy Hasti Mandiri Mandiri, Listy Hasti Maryana, Windri Masir, Rizkya A. Mauly Halwat Hikmat Mazwar Ismiyanto Miftakhul Huda Miftakhul Huda Minallah, Aldin Nasrun Muammar Fawwaz Muhammad Izzul Haq Muhammad Nizar Muhammad Noor Kholid Muhammad Samsuri Muhammad Syahriandi Adhantoro Munawaroh , Rima Muslimawati, Dinny Patria Nabila Fitriyani Nanang Mulyana, Nanang Naufal Ishartono Naufal Ishartono Ningtyas, Yoga Dwi Windy Kusuma Ninik Nihayatul Wahibah Ninik Nihayatul Wahibah Nurhidayat Nurhidayat Nurul Hikmah Kartini Oktiatama, Firlana Bakti PUGUH KARYANTO Puguh Karyanto Puguh Karyanto Purnomo Purnomo Purnomo Purnomo Purwono , Bambang Putri Agustina Putri Agustina Putri Agustina Putri Agustina Putri Salwa Salsabilla Putri, Wiffy Zalina Rahayu, Triasuti Ratih Dewianty Ratnasari Diah Utami Rika Dini Saputri Risnasari, Wanda Datik Rizko Hadi Rizko Hadi, Rizko Rosyada, Alya Nugrahaeni Sabar Narimo Sabdina, Berlian Achya Putri Sajidan Sajidan Santhyami Santhyami Sari , Banuwati Kartika Seno, Hernandito Aryo Septinawati, Septinawati Setyabudi, Devary Pradana Sigit Dwi Maryanto Sigit Dwi Maryanto Sigit Dwi Maryanto Silviani, Defina Anggita Siti Kartika Sari Siti Nur Syarifah Siti Nur Syarifah Siti Nuraini Siti Nurjanah Sofyan Anif Sri Katoningsih Sri Sat Titi Hamranani Suparti Suparti Suparti Suparti Suparti Suparti Suryawati, Andari Tri Sutama Syarifah, Siti Nur Temon Bagus Hidayatullah Triastuti Rahayu Trio Ageng Prayitno, Trio Ageng Tyastuti, Erma Musbita Ulya, Nuriya Hazma Arifatul Vika Manda Putri Widi Widayat Yusnita Rahmawati Zainal Arifin Zakaria, Gamal Abdul Nasir Zulperi, Dzarifah