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All Journal Prosiding Seminar Biologi Bio-Pedagogi : Jurnal Pembelajaran Biologi AL KAUNIYAH IPTEK Journal of Proceedings Series Bioeksperimen: Jurnal Penelitian Biologi Pharmacon Scientiae Educatia: Jurnal Pendidikan Sains BIOTROPIC The Journal of Tropical Biology BIOEDUSCIENCE BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains Journal of Education Technology Indonesian Journal on Learning and Advanced Education (IJOLAE) Buletin KKN Pendidikan Jurnal Riset Biologi dan Aplikasinya Bioscientist : Jurnal Ilmiah Biologi Proceeding Biology Education Conference Abdi Teknoyasa Quagga: Jurnal Pendidikan dan Biologi International Conference on Education for All JIPI (Jurnal IPA dan Pembelajaran IPA) Journal of Biotropical Research and Nature Technology Sasambo: Jurnal Abdimas (Journal of Community Service) Proceeding of International Conference on Biology Education, Natural Science, and Technology
Yasir Sidiq
Universitas Muhammadiyah Surakarta
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Other

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Characterization and Molecular Identification of Lipolytic-bacterial Isolates Forming Biofilm on Polyethylene Plastic Ariyanti, Olivia Puja; Agustina, Putri; Anif, Sofyan; Seno, Hernandito Aryo; Hardianto, Alden Ganendra Madhava Priya; Mandiri, Listy Hasti; Zulperi, Dzarifah; Jamil, Nazia; Rahayu, Triastuti; Sidiq, Yasir
Jurnal Riset Biologi dan Aplikasinya Vol. 7 No. 1 (2025)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v7n1.p57-65

Abstract

Polyethylene (PE) plastic is one of the most widely used for multiple purposes and leading the environmental problems. Lipolytic bacteria are promising agents to reduce the plastic waste. The dry weight of PE plastic was reduced by 33 % in the Winogradsky's column after 45 days of incubation. However, the lipolytic bacteria responsible for those reduction was unknown. This study aimed to characterize and identify the potential lipolytic bacterial isolates forming biofilm on polyethylene PE plastics. Samples of lipolytic bacterial isolates were screened on tributyrin selective media based on the formed clear zone. Moreover, the 16S rRNA genes of the two most potential lipolytic bacterial isolates were amplified. Then, the amplicons of the 16S rRNA gene were sequenced. This study found two potential lipolytic bacteria isolates, AB A-2 and AB M-3, which had the characteristics of round colonies, wavy edges, convex surfaces, and milky white color. The two isolates are gram-positive and have the shape of Coccobacillus cells. The molecular identification showed that AB A-2 isolate was Bacillus sp., while AB M-3 isolate was Bacillus amyloliquefaciens. This finding contributes to novel bacterial isolates that potentially overcoming the plastic waste problem.
Pelatihan Pengembangan Multimedia Pembelajaran Interaktif di SMP Mutu Menggunakan Articulate Storyline Guntur Nurcahyanto; Djumadi Djumadi; Hariyatmi Hariyatmi; Putri Agustina; Yasir Sidiq; Annur Indra Kusumadani; Mazwar Ismiyanto; Arum Dyah Ripdiyanti
Sasambo: Jurnal Abdimas (Journal of Community Service) Vol. 5 No. 1 (2023): Februari
Publisher : Lembaga Penelitian dan Pemberdayaan Masyarakat (LITPAM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/sasambo.v5i1.1052

Abstract

SMP Muhammadiyah 7 Program Unggulan Colomadu (SMP MUTU) mencanangkan program unggulan berupa pembelajaran berbasis teknologi informasi. Namun, keterampilan guru dalam hal mengembangkan multimedia pembelajaran interaktif berbasis Information Communication and Technology (ICT) masih perlu ditingkatkan. Oleh karena itu, kegiatan ini bertujuan untuk meningkatkan kemampuan guru dalam mengembangkan multimedia pembelajaran interaktif. Multimedia interaktif dikembangkan menggunakan aplikasi Articulate Storyline versi 3.12. Kegiatan ini meliputi empat tahapan umum, yaitu (1) sosialisasi dan persiapan, (2) penyiapan modul pelatihan, (3) pelaksanaan, dan (4) evaluasi dan monitoring. Empat tahap tersebut dilaksanakan secara tatap muka dan virtual dan diikuti oleh 12 guru SMP MUTU. Hasil evaluasi menunjukkan bahwa nilai penguasaan materi pelatihan oleh guru berkisar antar 67,85 sampai dengan 100. Sedangkan kategori nilai individu guru tentang penguasaan materi pelatihan adalah baik dengan jumlah 3 orang dan sangat baik dengan jumlah 9 orang. Pada akhir kegiatan, lebih dari 83% guru merespon kegiatan ini dengan sangat baik dalam hal peningkatan kualitas multimedia pembelajaran interaktif. Pelatihan meningkatkan kemampuan guru di SMP MUTU dalam mengembangkan multimedia pembelajaran interaktif berbasis ICT. Training on Development of Interactive Learning Multimedia at SMP MUTU Using Articulate Storyline   Teachers' skills at SMP Muhammadiyah 7 Colomadu (SMP MUTU) in developing technology-based interactive learning multimedia need to be improved. This program aims to provide a training to the teachers of SMP MUTU in developing interactive learning multimedia. Interactive multimedia was developed using the Articulate Storyline application version 3.12. This program includes four general stages, namely (1) socialization of activities, (2) preparation of training modules, (3) implementation, and (4) evaluation. The four stages were carried out by face-to-face and virtual methods. The results of the evaluation showed that the value of mastery of training materials by teachers ranged from 67.85 to 100 scores. In addition, three teachers belong to the category of good and nine teachers belong to the category of excellent in terms of mastery of training materials. Also, more than 83% of teachers responded very well to this program in terms of improving the quality of interactive learning multimedia. In summary, this program can provide significant understanding and experience to teachers at SMP MUTU in developing interactive learning multimedia
Rhizosphere Bacteria Increased the Height and Root Number of Chili Plants (Capsicum annuum L.) Nofa Anjani; Triastuti Rahayu; Yasir Sidiq
Scientiae Educatia: Jurnal Pendidikan Sains Vol 13, No 1 (2024): June 2024
Publisher : Tadris Biologi Fakultas Ilmu Tarbiyah dan Keguruan IAIN SYEKH NURJATI CIREBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24235/sc.educatia.v13i1.15158

Abstract

The need for potential bacteria as plant-promoting agents is still in high demand. Previous reports suggested five potential rhizobacteria isolated from the frangipani plant (Pulmeria accuminata) and producing indole acetic acid (IAA). However, evidence of its potential as a plant growth promoter has not been obtained. Therefore, this study aims to test the effect of rhizosphere bacterial inoculation on chili plants (Capsicum annuum L.). The method was a quantitative experimental method, with 15 repetitions for each treatment. The data were analyzed using a 95% student t-test confidence test or a significant level of 5% (a = 0.05). This inoculation begins with the germination of chili seeds until the radicula is observed. The germinated chili seedlings were soaked in bacterial suspension for an hour at room temperature. The results showed that inoculation using P38 isolate incubated for 6 hours significantly increased plant height and number of roots. The P31 isolate that was incubated for 6 hours inhibited the height of plants and the number of leaves of chili plants. Thus, these results provide important information about the effect of inoculation of newly isolated rhizosphere bacteria.
Phosphate Solubilisation Index and Antagonism Potential of Frangipani Tree Rhizosphere Bacterial Isolates from Cemetery Vika Febriyanti; Erma Musbita Tyastuti; Yasir Sidiq; Triastuti Rahayu
Proceeding of International Conference on Biology Education, Natural Science, and Technology 2023: Proceeding of International Conference on Biology Education, Natural Science, and Technology
Publisher : Universitas Muhammadiyah Surakarta

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Abstract

Frangipani trees (Plumeria acuminata) are commonly found in cemeteries. Cemeteries are used as a location for interment so that the corpse actively decomposes to provide soil minerals that encourage the formation of microbes, including bacteria. Bacteria around the roots of frangipani trees are thought to have potential as plant growth-promoting rhizobacteria (PGPR). Based on reference searches, there has been no research on frangipani tree rhizosphere bacteria from burial grounds. The aim of this study was to investigate the phosphate-dissolving and blast disease-causing Pyricularia oryzae fungus-inhibiting properties of frangipani tree rhizosphere bacteria from Pracimaloyo cemetery, Surakarta. A total of 39 isolates of frangipani tree rhizosphere bacteria were tested for their ability to dissolve phosphates using PKA media (Pikovskaya), while the antagonism test against the fungal pathogen Pyricularia oryzae used the dual assay method. The results of the study showed that 15% of the rhizosphere bacteria of the frangipani tree were able to dissolve phosphate, and 33% of the isolates were able to inhibit the growth of the fungus Pyricularia oryzae with an inhibitory power of more than 40%. Based on the results, the rhizosphere bacterial isolates from Pracimaloyo TPU show the characteristics of a possible PGPR.
Isolation and Characterization of Cellulolytic Bacteria from Bonoloyo, Cemetery Wanda Datik Risnasari; Erma Musbita Tyastuti; Yasir Sidiq; Triastuti Rahayu
Proceeding of International Conference on Biology Education, Natural Science, and Technology 2023: Proceeding of International Conference on Biology Education, Natural Science, and Technology
Publisher : Universitas Muhammadiyah Surakarta

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Abstract

A public cemetery (TPU) is land provided by the government to bury bodies. The culture of funerals in Indonesia is varied but generally involves being buried in the soil with wrapped clothes, covered wooden boards, and tombs. The material contains cellulose that will be broken down by cellulolytic microorganisms, including bacteria. The exploration of the cellulolytic bacteria of TPU in Indonesia has not been carried out, so this study aims to investigate and identify cellulose bacteria isolates from TPU. This research is a non-experimental study for the isolation of bacteria in the soil of TPU Bonoloyo, Surakarta. A selection of cellulite bacteria is grown in CMC media. Isolates that show cellulolytic activity are identified by observing colonial morphology and Gram coloring. Selection results of cellulolytic bacteria obtained isolates with cellulolytic index (IS) including a high category of 1 isolate (B14), a medium category of 3 isolates (B16, B27, and B45), and 9 low category isolates. The study concluded that 28.8 % showed cellulolytic activity with irregular morphological forms of white dyeing on flat surfaces with flat edges and dominated by gram-negative coccyx-shaped.
Isolation and Identification of Soil Bacteria in Pracimaloyo Public Cemetery, Kartasura Bagas Adityaradja; Erma Musbita Tyastuti; Yasir Sidiq; Triastuti Rahayu
Proceeding of International Conference on Biology Education, Natural Science, and Technology 2023: Proceeding of International Conference on Biology Education, Natural Science, and Technology
Publisher : Universitas Muhammadiyah Surakarta

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Abstract

In The Pracimaloyo Public Cemetery, the body’s decomposition occurs continuously, producing soil nutrients and minerals that affect the growth of bacteria. Bacterial population data from the cemetery area is still very limited, whereas cemeteries hold the risk of contamination with pathogenic bacteria. This research aims to find out how the population and diversity of bacteria in the Pracimaloyo Public Cemetery. Soil samples were taken from the Pracimaloyo cemetery at 2 locations (blocks 8 and 18), each at 20 and 50 cm depth. Soil samples are inoculated in Nutrient Agar media using the spread plate method. After 48 hours, colony counting, colony morphology, and gram staining observations were carried out. The rate of soil bacterial populations in blocks 8 and 18 at a depth of 20 cm was 4.23 × 107 CFU/g and 9.79 × 107 CFU/g, while at depths of 50 cm, it was 1.94 × 107 CFU/g and 1.92 × 107 CFU/g. The morphology of the bacterial colonies is dominated by circular shape, entire margin, flat elevation, and white color. 20 isolates are gram-negative and 16 isolates are gram-positive, the cell form is dominated by the bacillus.
Quantitative Traits of Melodi Gama-3 Melon (Cucumis Melo L.), A New Superior Cultivar, on Rainy Season and Altitude Variation Hadi, Rizko; Sidiq, Yasir; Maryanto, Sigit Dwi; Daryono, Budi S
Journal of Biotropical Research and Nature Technology Vol. 1 No. 2 (2023): Borneo
Publisher : Prodi Biologi FMIPA Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52850/borneo.v1i2.8998

Abstract

Melodi Gama 3 is a superior melon cultivar candidate which was developed presently. Purposes of this study were to determine quantitative traits of the Melodi Gama 3, as well as to compare it to commercial melon cultivars, based on trial on rainy season and altitude variation. Materials used were Melodi Gama-3 seeds originated from Laboratory of Genetics, Faculty of Biology, Universitas Gadjah Mada, and commercial cultivars seeds, i.e., Action 434, Glamour Sakata, MAI, and LADIKA, respectively. The trial had been conducted at three variations of altitude i.e., mediumland, lowland, and beach shore, respectively, in period of rainy season. The quantitative traits, that were fruit weight and girth, flesh, and rind thickness, as well as vertical and horizontal diameter (longitudinal section), respectively, had been observed and measured at the Laboratory of Genetics, Faculty of Biology, Universitas Gadjah Mada. Data then analyzed with Honestly Significant Different test following balanced ANOVA, all using PKBT-STAT 2 software. Results showed that all the quantitative traits, except for flesh thickness, were influenced very significantly by interaction of cultivars x locations (P < 0.01). The Honestly Significant Difference test indicated that the Melodi Gama-3 had higher measurement of its quantitative traits (fruit weight: 2.68 kg; fruit girth: 55.45 cm; rind thicknesss: 0.93 – 1 cm; vertical diameter: 15.94 – 18.19 cm; horizontal diameter: 17.79 cm) than other cultivars. It was revealed that the Melodi Gama-3 was best planted at lowland altitude. Plant breeders should benefit from this study for the Melodi Gama-3 has great potency to be bred based on its traits comparison with commercial cultivars.
Co-Authors A.A. Ketut Agung Cahyawan W Adilla Nur Azizah Alanindra Saputra Alanindra Saputra Alanindra Saputra Alanindra Saputra Anatri Desstya Anggita Juniar Laspartriana Anggun Dwi Nur Annisa Annur Indra Kusumadani Apriliana, Cindi Dila Ariyanti, Olivia Puja Arum Dyah Ripdiyanti Azizah, Adilla Nur Bagas Adityaradja Bambang Harmanto, Bambang Bambang Sumardjoko Baskoro Adi Prayitno Baskoro Adi Prayitno Bowo Sugiharto Bowo Sugiharto Bowo Sugiyarto BUDI SETIADI DARYONO Condro Nur Alim D Djumadi Daryono, Budi S Dedi Gunawan Defina Anggita Silviani Devary Pradana Setyabudi Dicky Eka Prasetya Donny Widianto Donny Widianto Dyah Astuti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Erma Musbita Tyastuti Evi Dwi Kartikasari Fari Fadilah Febrianti, Nisa Gamal Abdul Nasir Zakaria Ganno Tribuana Aji Guntur Nurcahyanto Hardianto, Alden Ganendra Madhava Priya Hariyatmi Hariyatmi Harun Joko Prayitno Heri Siswanto Hernawan Sulistyanto Ihsan Nugroho Irfadhila, Dwega Izzatul Azmi Mulya Jakia, Nikmatu Jamil, Nazia Junita Dwi Wardhani Kistantia Elok Mumpuni Kistantia Elok Mumpuni Kistantia Elok Mumpuni Listy Hasti Mandiri Listy Hasti Mandiri Mandiri, Listy Hasti Maryana, Windri Masir, Rizkya A. Mauly Halwat Hikmat Mazwar Ismiyanto Miftakhul Huda Minallah, Aldin Nasrun Muhammad Izzul Haq Muhammad Nizar Muhammad Noor Kholid Muhammad Samsuri Muhammad Syahriandi Adhantoro Muslimawati, Dinny Patria Nabila Fitriyani Naufal Ishartono Ningtyas, Yoga Dwi Windy Kusuma Ninik Nihayatul Wahibah Nofa Anjani Nurhidayat Nurhidayat Nurul Hikmah Kartini Oktiatama, Firlana Bakti Puguh Karyanto Puguh Karyanto PUGUH KARYANTO Purnomo Purnomo Purnomo Purnomo Putri Agustina Putri Agustina Putri Agustina Putri Salwa Salsabilla Putri, Vika Manda Qori Tsaniyah Ainun Insani Ratih Dewianty Ratnasari Diah Utami Rika Dini Saputri Rizko Hadi Rizko Hadi, Rizko Sabar Narimo Seno, Hernandito Aryo Septinawati, Septinawati Sigit Dwi Maryanto Sigit Dwi Maryanto Sigit Dwi Maryanto Siti Kartika Sari Siti Nur Syarifah Siti Nur Syarifah Siti Nuraini Siti Nurjanah Sofyan Anif Sri Katoningsih Sri Sat Titi Hamranani Suparti Suparti Suparti Suparti Suryawati, Andari Tri Sutama Temon Bagus Hidayatullah Triastuti Rahayu Trio Ageng Prayitno, Trio Ageng Ulya, Nuriya Hazma Arifatul Vika Febriyanti Vina Listiawati Wanda Datik Risnasari Widi Widayat Yusnita Rahmawati Zainal Arifin Zulperi, Dzarifah