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All Journal HAYATI Journal of Biosciences Jurnal Teknologi Dan Industri Pangan Jurnal Teknologi Industri Pertanian Jurnal Pengolahan Hasil Perikanan Indonesia ADITYA - Pendidikan Bahasa dan Sastra Jawa Jurnal Veteriner Jurnal Ilmu Ternak Microbiology Indonesia Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Jurnal Sumberdaya Hayati Indonesian Journal of Cancer Jurnal SEPA (Social Economic and Agribusiness Journal) Biosaintifika: Journal of Biology & Biology Education TEKNOLOGI PANGAN : Media Informasi dan Komunikasi Ilmiah Teknologi Pertanian Jurnal Sains Komputer dan Teknologi Informasi BioEksakta : Jurnal Ilmiah Biologi Unsoed Indonesian Journal of Cancer Makara Journal of Science Jurnal Pendidikan Nasional Jurnal Inovasi Penelitian Ilmu Pendidikan Indonesia Bioma : Jurnal Ilmiah Biologi Kesmas: Jurnal Kesehatan Masyarakat Nasional (National Public Health Journal)
Sri Budiarti Poerwanto
Department Of Biology, Faculty Of Mathemathics And Natural Sciences, Bogor Agricultural University, Darmaga Campus, Bogor 16680
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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PEMURNIAN DAN KARAKTERISASI INHIBITOR PROTEASE DARI Chromohalobacter sp. 6A3, BAKTERI YANG BERASOSIASI DENGAN SPONS Xetospongia testudinaria [Purification and Characterization of Protease Inhibitor from Chromohalobacter sp. 6A3, Bacteria-associated with S Tati Nurhayati; Maggy T. Suhartono; Lilis Nuraida; Sri Budiarti Poerwanto
Jurnal Teknologi dan Industri Pangan Vol. 21 No. 2 (2010): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

 Various sponges has been reported to produce protease inhibitor which could inhibit protease activity of pathogenic bacteria. The previous research showed that bacteria-associated with sponge could produce bioactive compound similar to their host, including protease inhibitor. The purposes of this research were to purify protease inhibitor from Chromohalobacter sp. 6A3 and to study the characteristics of the protease inhibitor. The result showed that the protein can be extracted by 30 % (v/v) acetone, purified by gel filtration (Sephadex G-75) and finally, purified by anion exchanger (Sephadex A-50). The molecular weight of the purified protease inhibitor after gel filtration was estimated as 21,31 kDa and 17,05 kDa, but anion exchanger gave protein with estimated molecular weight of 21,31 kDa The optimum temperature and pH were 30 oC and 5 respectively. The protease inhibitor could resist heating at 30 oC for 10 minutes. Incubation of the inhibitor at 30 oC, pH 5, still retained its activity until 3 hours. The purified enzyme inhibitor was also still active after incubated at pH from 5 to 6 for 1 hour. The most susceptible substrate (enzyme) for the inhibitor was protease from P. aeruginosa. The protease inhibitor was inhibited by metal ions except Na+ 1mM. Activity of the inhibitor increased twofold by SDS 5 mM. IC 50 of the protease inhibitor was 3.48 nM. The protease inhibitor inhibited the enzyme uncompetitively.
Influence Of Glucose And Yeast Extract Toward Production Of Pseudomonas Aeruginosa-Protease Inhibitor From Chromohalobacter Sp. 6a3 (Bacteria Associated With Sponge Xetospongia Testudinaria) Tati Nurhayati; Maggy Thenawidjaja; Lilis Nuraida; Sri Budiarti Poerwanto
Jurnal Teknologi Industri Pertanian Vol. 19 No. 2 (2009): Jurnal Teknologi Industri Pertanian
Publisher : Department of Agroindustrial Technology, Bogor Agricultural University

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Abstract

One way to inhibit protease activity is search is compound which can inhibit the enzyme known as protease inhibitor. The bacteria associated with sponge Xetospongia testudinaria, Chromohalobacter sp. 6A3, as producer Pseudomonas aeruginosa-protease inhibitor. Because the compound is important, determination medium composition for producing is very important to be conducted.  The purpose of this research was to determinate the glucose and yeast extract consentration accurately so protease inhibitor would be produced in a short time.  The accurate medium composition for producing the protease inhibitor were 0.1%(w/v) yeast extract; 0.05% (w/v) glucose;  0.5%(w/v) special peptone; 0.2%(v/v) trace element; and 2%(w/v) NaCl at pH 7.Keywords: Chromohalobacter sp., protease inhibitor, sponges.
Aplikasi Bakteri Asam Laktat dari Inasua sebagai Biopreservatif Ikan Patin (Pangasius sp.) : Lactic Acid Bacteria Aplication from Inasua As Biopreservative for Catfish (Pangasius sp.) Meilany Ariati Dewi; Nisa Rachmania Mubarik; Desniar Desniar; Sri Budiarti
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 25 No 1 (2022): Jurnal Pengolahan Hasil Perikanan Indonesia 25(1)
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v25i1.39206

Abstract

Lactic Acid Bacteria or LAB are bacteria that are categorized as generally recognized as safe (GRAS) because they are safe for human health and are non-pathogenic, so they have the potential to be biopreservative. Several metabolic products produced by LAB have antibacterial properties, including bacteriocin, diacetyl, hydrogen peroxide and organic acids. Isolates of L. plantarum IN05 and L. rhamnosus IN13 isolated from fermented inasua food were studied for their potential as biopreservatives because of their antibacterial properties. The content of animal protein in fish is in great demand by the public, one of which is catfish which is in great demand by the public, but catfish is easy to damage and decrease in quality. This is due to the presence of the pathogenic bacterium Listeria monocytogenes which has the ability to grow at cold storage temperatures (refrigeration) to freezing. This study aimed to apply a neutral cell-free supernatant, cell-free supernatant, and bacterial cell biomass from L. rhamnosus IN13 and L. plantarum IN05 isolates and formalin in storage of catfish fillets contaminated with Listeria monocytogenes bacteria. The methods used include the production of antibacterial compounds, determination of the sensitivity of antibacterial compounds to test bacteria, confirmation test of antibacterial compounds with the addition of proteinase K, application of antibacterial compounds to catfish fillets, calculation of total bacterial colonies, bacterial colonies of L. monocytogenes, and lactic acid bacteria, as well as measurement of the pH value of catfish fillet. The SBSN antibacterial compound from L. rhamnosus IN13 has the ability to maintain catfish fillets for up to 14 days of storage in accordance with the requirements for fresh fish that are suitable for consumption according to the 2017 SNI standard on general requirements and guidelines for microbiological testing. The total microbial value was 5.65 log CFU/g, the pH value of catfish fillet meat was 6.37, and the total value of L. monocytogenes bacteria decreased during storage time.
Penggunaan Interjeksi dalam Novel Nona Sekretaris Karya Suparto Brata Sri Budiarti
ADITYA - Pendidikan Bahasa dan Sastra Jawa Vol 7, No 1 (2015): ADITYA
Publisher : Pendidikan Bahasa dan Sastra Jawa

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Abstract

Abstrak: penelitian ini bertujuan: (1) mendeskripsikan bentuk interjeksi dalam novel Nona Sekretaris karya Suparto Brata; (2) mendeskripsikan fungsi interjeksi dalam novel Nona Sekretaris karya Suparto Brata, yaitu (a) bentuk primer (o, lho, ah, lo, heh, wah, la, oh, ei, ha, wo, e, hiss, huh, hee, huuuh, oo, huss, wih), (b) bentuk sekunder (ayo, buset, stop, gendheng, athoo, embuh, hebat, edan, wahdhuh, toblas, emoh, adhuh, kapok, bodho, astagfirullah, bangsat), (c) bentuk pengulangan kata (ah-ah, apik-apik, ala-la-la, his-his, ala-la-la, e-e). Bentuk interjeksi memiliki fungsi berbeda-beda: (1) (a) interjeksi keheranan, (b) interjeksi kekaguman, (c) interjeksi kekesalan, (d) interjeksi panggilan, (e) interjeksi mengeluh, (f) interjeksi kekagetan, (g) interjeksi pujian, (h) interjeksi kemarahan, (i) interjeksi kesedihan, (j) interjeksi tidak senang, (k) interjeksi kesenangan, (l) interjeksi harapan, (m) interjeksi kekecewaan, (n) interjeksi harapan, (o) interjeksi ajakan, (p) interjeksi kesakitan, (q) interjeksi makian.   Kata kunci: interjeksi, novel Nona Sekretaris
Pemurnian Ekstraseluler Hyaluronidase Streptococcus agalactiae (Streptokokus Grup B ) (Extracellular Hyaluronidase Purification of Streptococcus agalactiae (Group B of Streptococus) Wendry Setiyadi Putranto; Sri Budiarti; Maggy T. Suhartono; I Wayan T. Wibawan; Zainatul Hayati
Jurnal Ilmu Ternak Vol 6, No 1 (2006)
Publisher : Fakultas Peternakan, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jit.v6i1.2260

Abstract

Hyaluronidase (EC 4.2.2.1) merupakan enzim ekstraseluler yang dihasilkan Streptococcus agalactiae (Streptokokus Grup B). Penelitian ini bertujuan untuk mengetahui aktivitas spesifik dari hyaluronidase S. agalactiae dan berat molekul proteinnya. Pemurnian enzim dengan sentrifugasi, pengendapan amonium sulfat 45% dan kromatographi kolom dengan Sephadex G-100 dan Sodiumdodecyl sulfate polyacrylainide gel electrophoresis (SDS PAGE). Enzim hyaluronidase dari S. Agalactiae memiliki aktivitas spesifik sebesar 0,0012 U/mg (ekstrak kasar), 0,0125 U/mg (pengendapan dengan 45% ammonium sulfate) and 0,032 U/mg (Gel Filtration). Berat molekul protein hyaluronidase adalah 102 - 106 kD.Kata kunci : hyaluronidase, aktivitas spesifik, pemurnian
AKTIVITAS HIALURONIDASE BAKTERI STREPTOKOKUS GRUP B PADA SUBSTRAT ASAM HIALURONAT THE HYALURONIDASE ACTIVITY OF GROUP B STREPTOCOCCUS IN HYALURONIC ACID SUBSTRATE Zinatul Hayati; Wendy Setiyadi Putranto; Teuku Fadrial Karmil; I Wayan Teguh Wibawan; Sri Budiarti Poerwanto
Jurnal Veteriner Vol 8 No 2 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Streptococcus agalactiae atau yang juga dikenal dengan Streptokokus grup B (SGB) telah diketahui sebagai agen penyebab pneumonia, septikemia dan meningitis neonatal pada manusia dan hewan. Hialuronidase merupakan produk ekstraseluler dari SGB yang menentukan virulensinya. Penelitian ini dilakukan untuk menguji akitvitas hialuronidase dari 10 isolat SGB yang diisolasi dari kasus komplikase obstetri, serta memurnikan dan mengkarakterisasi hialuronidase yang diisolasi dari SGB SV-14 pada substrat asam hialuronat.
Analysis of Rumen Microbial Population of Cattle Given Silage and Probiotics Using Terminal Restriction Fragment Length Polymorphism RONI RIDWAN1; YANTYATI WIDYASTUTI1; SRI BUDIARTI; ACHMAD DINOTO
Microbiology Indonesia Vol. 3 No. 3 (2009): December 2009
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (320.18 KB) | DOI: 10.5454/mi.3.3.2

Abstract

Rumen ecology is an important observation in evaluating the effectivity of silage and probiotic additives relating to their roles in cattle productivity. The objective of this study was to examine the effects of silage and probiotics on ruminal ecosystems in vivo using a molecular approach. Terminal-restriction fragment-length-polymorphism (T-RFLP) analysis was used to detect changes of ecological communities based on 16S-ribosomal deoxyribonucleic acid (16S-rDNA). Two rumen canulated PO cattle were fed several diets i.e.; (R0) basal diet dry matter basis (Pennisetum purpureum 70% and commercial concentrate 30%), (R1) silage (basal diet fermented using Lactobacillus plantarum BTCC570), (R2) silage + probiotics (L. plantarium Str BTCC531), (R3) Basal diet + probiotics (L. plantarium Str BTCC531). Digesta samples were collected 3 h after feeding for pH and T-RFLP analysis. T-RFLP analysis was performed using the 16S-rDNA amplified from each sample. The lengths of the terminal restriction fragments were analysed after digestion with HhaI, HaeIII and MspI. Results showed the effectivenes of silage and probiotics, given together, on the index of Smith and Wilson evenness applied to T-RFLP ecology data (Evar) with 0.89±0.04 being the highest. The diversity of rumen microorganisms is influenced by individual differences of each animal. T-RFLP analysis has a potency to be used for comparisons of complex bacterial communities, especially to detect changes in community structure in response to different variables and to show rumen bacteria diversity in the rumen.
KAJIAN PARAMETER KEBERADAAN VEKTOR PENYAKIT DEMAM BERDARAH DENGUE (DBD) MENGGUNAKAN DUKUNGAN PENGINDERAAN JAUH (REMOTE SENSING) DI KOTA PONTIANAK Asmadi Asmadi; Akhmad Arif Amin; Sri Budiarti; Machmud Arifin Raimadoya
Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Vol. 1 No. 1 (2011): Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (JPSL)
Publisher : Graduate School Bogor Agricultural University (SPs IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jpsl.1.1.16

Abstract

Dengue Hemorrhagic Fever (DHF) transmission dynamic is naturally influenced by fluctuating environmental conditions that could be locally specific even at the level of urban areas. Remote sensing technology is increasingly recognized as a powerful tool to scan DHF endemic areas and could be used to monitor DHF vectors fluctuation and the related biogeographical environment. A study was conducted to estimate the DHF transmission intensity in four endemic distric in Pontianak urban areas West Borneo. The estimation was based on environmental condition and the most of the data were obtained through remote sensing using the satellite IKONOS and NOAA. The study subjects were the environmental conditions of the urban areas considered as the risk factors for DHF transmission. Data were collected either through field observations and remote sensing. Data set was analyzed with the discriminant analysis module using the SPSS 17.0. The results of the study showed that there were predictor variables of the environment risk factors should be considered in the estimation of DHF transmission intensity in certain DHF endemic distric. Those variables included: (1) air temperature, (2) mosquito vector density, (3) relative humidity and (4)building density. Linear discriminant function was obtained to predict the incidence of DHF outbreak. Applying this model, DHF transmission intensity in certain distric could be estimated with a high accuracy. The result showed that the assessment model could be built following the formula:Y = 237,490 + 113,474 x (vector) – 121,828 x (temperature) – 98,999 x (relative humidity) + 78,782 x (building) that could be as high accuracy as 90,9 %. Keywords: Remote sensing technology, Dengue Hemorrhagic Fever (DHF), DHF transmission intensity, Mosquito vektor density, IKONOS, NOAA
ANALISIS EKSPOR JAHE DARI JAWA TENGAH Sri Budiarti; Heru Irianto
SEPA: Jurnal Sosial Ekonomi Pertanian dan Agribisnis Vol 1, No 2 (2005): FEBRUARY
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/sepa.v1i2.48788

Abstract

The research aims to know factors that influance ginger export from Central Java and supply elasticity of ginger export. Basic method used in the research is descriptive analytis and area sampling with puposive. Analysis method use non linear regretion model
Peran Ekstrak Etanol Kulit Manggis (Garcinia mangostana L.) Dalam Menginduksi Apoptosis Sel Kanker Lidah Manusia Sp-C1 In Vitro SUPRIATNO -; HENDRI SUSANTO; SRI BUDIARTI
Indonesian Journal of Cancer Vol 7, No 4 (2013): Oct - Dec 2013
Publisher : National Cancer Center - Dharmais Cancer Hospital

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33371/ijoc.v7i4.309

Abstract

Sel kanker lidah mempunyai karakteristik pertumbuhan sel yang cepat, invasi, dan metastasis yang tinggi. Penatalaksanaan untuk kanker primer, metastasis, dan residif masih menunjukkan hasil yang belum memuaskan sehingga dipikirkan untuk mengombinasi dengan terapi pengobatan alternatif menggunakan bahan alam kulit manggis (Garcinia mangostana L.). Tujuan penelitian menguji induksi apoptosis sel kanker lidah Sp-C1 menggunakan ekstrak etanol kulit manggis in vitro. Induksi apoptosis sel pasca-perlakuan ekstrak etanol kulit manggis konsentrasi 0, 2,5, 5, 7,5, 10, dan 12,5 ?g/ml dilakukan menggunakan uji kolorimetrik caspase-3 dan -9 (DVED-pNA dan LEHD-pNA). Data dianalisis menggunakan Anova satu jalur, dilanjutkan dengan uji Post-hoc LSD dengan derajat kemaknaan 95%. Hasil penelitian menunjukkan ekstrak etanol kulit manggis konsentrasi 12,5 ?g/ml menginduksi apoptosis sel kanker lidah manusia Sp-C1 melalui aktivitas proteolitik caspase-3 dan caspase-9 (P=0,001). Peningkatan kelipatan aktivitas proteolitik caspase-3 dan -9 diketahui sebesar 1,39 dan 2,15 kali lipat. Kesimpulannya, ekstrak etanol kulit manggis dapat menginduksi apoptosis sel kanker lidah manusia Sp-C1.Kata Kunci: sel kanker lidah Sp-C1, kulit manggis, apoptosis, caspase-3 dan -9.
Co-Authors A M Sabbah, Sajida ACHMAD DINOTO ALEX HARTANA Alex Hartana Alex Hartana ALEX HARTANA ALEX HARTANA ANIK KUSMIATUN Asmadi Asmadi Asmendri Asmendri Bambang Pramudya Bambang Pramudya Bambang Suryobroto Budi Wirawan Damaini, Amaya Andri Demina Desniar - - DEWI SARTIKA DITA, SASMIATI FARACH Efendi, Firda Sri Elda Irma Jeanne Joice Elda Irma Jeanne Joice Kawulur Fahrurroji Fitriandini, Risa Getriani, Zerina Harsi D. Kusumaningrum Hasim Hasim Hasim Hasim Hendri Susanto Hendri Susanto Henri Peranginangin Henri Peranginangin Heru Irianto I Wayan T. Wibawan I wayan Teguh Wibawan Iman Rusmana Jepri Agung Priyanto, Jepri Agung Katupayan, Sisilia Lilis Nuraida Machmud Arifin Raimadoya MAGGY T. SUHARTONO Maggy T. Suhartono Maggy Thenawidjaja Suhartono Meilany Ariati Dewi MIRNAWATI SUDARWANTO NISA RACHMANIA MUBARIK Niza, Yesita Roza Normalia, Normalia Nuraeni, Siti Panesya Oktavia, Wiwik Oktonuah, Elvandro Rahmiati, Retnani Rahminiwati, Min Reflinda Retnani Rahmiati Rifky, Muhammad Rika Indri Astuti RONI RIDWAN1 Saputri, Sindi Sari, Mila Supriatno Supriatno - Sutandi, Michellia Salmaa Ashilla TATI NURHAYATI TETRI WIDIYANI Teuku Fadrial Karmil Wendry Setiyadi Putranto Wendy Setiyadi Putranto Wibowo, Muhammad Arya Winiati P Rahayu Winiati P Rahayu YANTYATI WIDYASTUTI1 YULIN LESTARI Zainatul Hayati Zinatul Hayati Zulhaerati Zulhaerati