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All Journal HAYATI Journal of Biosciences Jurnal Teknologi Dan Industri Pangan Jurnal Teknologi Industri Pertanian Jurnal Pengolahan Hasil Perikanan Indonesia ADITYA - Pendidikan Bahasa dan Sastra Jawa Jurnal Veteriner Jurnal Ilmu Ternak Microbiology Indonesia Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Jurnal Sumberdaya Hayati Indonesian Journal of Cancer Jurnal SEPA (Social Economic and Agribusiness Journal) Biosaintifika: Journal of Biology & Biology Education TEKNOLOGI PANGAN : Media Informasi dan Komunikasi Ilmiah Teknologi Pertanian Jurnal Sains Komputer dan Teknologi Informasi BioEksakta : Jurnal Ilmiah Biologi Unsoed Indonesian Journal of Cancer Makara Journal of Science Jurnal Pendidikan Nasional Jurnal Inovasi Penelitian Ilmu Pendidikan Indonesia Bioma : Jurnal Ilmiah Biologi Kesmas: Jurnal Kesehatan Masyarakat Nasional (National Public Health Journal)
Sri Budiarti Poerwanto
Department Of Biology, Faculty Of Mathemathics And Natural Sciences, Bogor Agricultural University, Darmaga Campus, Bogor 16680
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Craniofacial Shape of Arfak People Based on Geometric Morphometric Features Kawulur, Elda Irma Jeanne Joice; Suryobroto, Bambang; Budiarti, Sri; Hartana, Alex
Makara Journal of Science Vol. 22, No. 1
Publisher : UI Scholars Hub

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Face and cranial (craniofacial) shape is highly specific to the individual; therefore, craniofacial shape is often used to identify individuals and to analyze variability in the human population. Previous studies, consisting only of verbal descriptions, suggested that the cranial shape of the Papuan people was highly variable. Despite their usefulness, verbal descriptions cannot fully demonstrate common and local variation in cranial shape. They also cannot be used to extractthe general trend of variation or to group face shapes based on their similarity. Here we attempt to apply geometric analysis, a method of shape analysis, to measure facial anatomical structural landmarks of Papuan people. The craniofacial shape of Papuan people was constructed from those of Arfak people based on 16 anatomic landmarks on the lateral side. Arfak is one of the traditional Papuan tribes in Manokwari, West Papua Province. Our result showed great variation in craniofacial shapes among the Arfak. The nose, chin, and mandible differed significantly, whereas other parts of the face were relatively stable and showed small variations. These differences reflected variations in the facial growth rate. The high level of diversity thus indicates that some parts of the face have higher plasticity in their growth pattern than others.
EVALUASI ANTARMUKA WEB BADAN PENGELOLA PAJAK DAN RETRIBUSI DAERAH KOTA PALANGKA RAYA Damaini, Amaya Andri; Oktonuah, Elvandro; Normalia, Normalia; Rifky, Muhammad; Katupayan, Sisilia; Budiarti, Sri
Jurnal Sains Komputer dan Teknologi Informasi Vol. 6 No. 1 (2023): Jurnal Sains Komputer dan Teknologi Informasi
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/jsakti.v6i1.5936

Abstract

Website SPPT PBB merupakan website yang dibuat oleh Badan Pengelola Pajak dan Retribusi Daerah (BPPRD) Kota Palangka Raya. Website ini dibuat agar pengisian SPPT PBB dan pencetakan STTS dapat dilakukan secara mandiri oleh masyarakat. Peneliti mengevaluasi website ini untuk mengetahui tingkat kepuasan. Tahapan penelitian yang digunakan adalah mengumpulkan data, kemudian melakukan uji usability, serta menganalisis dan mengevaluasi data. Berdasarkan hasil analisis data, berikut nilai usability berdasarkan variabel: Usefulness (72.11%), Ease of Use (70.15%), Ease of Learning (68.67%), and Satisfaction (70.42%). Jika dihitung nilai rata-ratanya maka hasilnya berada pada kategori layak (70,34%), atau dapat diartikan antarmuka website SPPT PBB dapat digunakan untuk menyelesaikan suatu tugas atau kegiatan. Terdapat satu variabel yang nilainya mendekati ambang batas bawah kategori layak yaitu Ease of Learning. Penelitian ini hanya memberikan rekomendasi perbaikan antarmuka website untuk meningkatkan nilai Ease of Learning. Rekomendasi ini diberikan dalam bentuk narasi. Untuk penelitian selanjutnya, peneliti berharap rekomendasi yang diberikan adalah desain user interface yang baru, sehingga apabila BPPRD ingin merombak website, sudah tersedia panduannya.
Sponge-Associated Actinobacteria: Morphological Character and Antibacterial Activity against Pathogenic Bacteria DITA, SASMIATI FARACH; BUDIARTI, SRI; LESTARI, YULIN
Jurnal Sumberdaya Hayati Vol. 3 No. 1 (2017)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.3.1.21-26

Abstract

Sponge-associated actinobacteria may diverse and have potency to produce bioactive compounds. Diversity and antimicrobial activity of indigenous sponge-associated actinobacteria isolated from the marine ecosystem in Indonesia have not much been explored. This work aimed to assess morphological and antibacterial activity of sponge-associated actinobacteria. The morphological characteristics were examined based on their color of aerial and substrate mycelia, and pigmentation, while antibacterial activities were assayed using the antagonist technique. The selected actinobacterial isolate was identified using 16S rRNA gene. Various sponge-associated actinobacteria were successfully isolated from Hyrtios sp., Callyspongia sp., and Neofibularia sp. sponges. A total of 62 actinobacterial isolates were obtained, and each isolate showed a variety of morphological characters, which could be seen in aerial mass color, substrate mass color, and pigmentation. Actinobacterial isolates were tested against human pathogenic bacteria, i.e. Staphylococcus aureus and Methicillin-Resistant S. aureus, representing Gram-positive, and Escherichia coli EPEC K1-1 and Shigella dysenteriae, representing Gram-negative. Most of actinobacterial isolates had antimicrobial activities at least against one of pathogenic bacteria. High activity was shown by NOHa.2, isolated from Neofibularia, and HRHa.5 isolated from Hyrtios. The NOHa.2 showed the highest antimicrobial activity against S. dysenteriae, meanwhile, HRHa.5 showed antimicrobial activity against 3 of 4 tested bacterial pathogens. These data showed diversity of sponge-asccociated actinobacteria from marine ecosystem in Indonesia, and several of them have potency as source of antibacterial compounds
Karakterisasi Bakteri Tanah Penghasil Enzim Hidrolitik yang Diisolasi dari Pulau Muna, Sulawesi Tenggara, Indonesia: Characterization of Hydrolytic Enzyme Producing-Bacteria Isolated from Soil of Muna Island, Southeast Sulawesi, Indonesia Wibowo, Muhammad Arya; Priyanto, Jepri Agung; Budiarti, Sri
Jurnal Sumberdaya Hayati Vol. 10 No. 2 (2024): 2024
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.10.2.55-60

Abstract

Soil bacteria are considered a promising source of extracellular enzymes, such as amylase, cellulase, and protease. In this study, 15 bacterial strains isolated from the soil of Muna Island, Southeast Sulawesi, Indonesia, were preliminary screened for amylolytic, cellulolytic, and proteolytic activities. The present study aimed to characterize cellular morphology, determine hemolytic characteristics, and to screen amylolytic, proteolytic, and cellulolytic activities of bacterial strains isolated from the soil of Muna Island. Based on the Gram staining procedure, 15 bacterial strains exhibited diverse cellular morphologies. Ten out of 15 strains belonged to Gram-positive bacilli, while the others belonged to Gram-negative bacteria (bacilli or cocci). Four strains, encoded as M7, P4, P5, and P7, were hemolytic negative. These potential strains exhibited different capabilities in producing extracellular enzymes. The four strains were capable of producing protease, while amylase and cellulase were only produced by two strains (M7 and P7). These strains have good prospects for further study and development for industrial and biotechnological applications.
Isolasi, Karakterisasi, dan Identifikasi Bakteri Penghasil Hemolisin dari Penderita Gingivitis: Isolation, Characterisation, and Identification of Hemolysin-Producing Bacteria from Gingivitis-Diagnosed Patient Sutandi, Michellia Salmaa Ashilla; Priyanto, Jepri Agung; Budiarti, Sri
Jurnal Sumberdaya Hayati Vol. 10 No. 4 (2024): 2024
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.10.4.162-169

Abstract

Gingivitis is a gum inflammation caused by pathogenic bacterial infection. One of the virulence factors of pathogenic bacteria is hemolysin which plays an important role in lysing the red blood cells. This study aimed to isolate, characterise, and identify hemolysin-producing bacteria from a gingivitis-diagnosed patient. Quantification of the bacterial growth in blood agar base media showed that the number of bacteria on the gums of gingivitis patient was 1.63 × 107 CFU/ml. Seven bacterial isolates with different colony and cellular morphology were selected. Of 7 isolates, 3 isolates were β hemolytic, 3 isolates α hemolytic, and 1 isolate non-hemolytic. Six isolates with hemolytic activity were selected for colony and cellular morphology characterisation. Based on Gram-staining procedure, all six isolates belong to bacilli Gram-positive bacteria. Molecular identification with 16S rRNA gene revealed that these isolates were closely related to Bacillus spp., such as Bacillus cereus, Bacillus altitudinis, and Bacillus tequilensis.
Characterization of Antibacterial Compounds from Marine Sponge-associated Streptomyces spp. against Some Pathogenic Bacteria Efendi, Firda Sri; Budiarti, Sri; Lestari, Yulin
HAYATI Journal of Biosciences Vol. 32 No. 1 (2025): January 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.1.55-69

Abstract

The increasing trend of antibiotic resistance among pathogenic bacteria is a worldwide problem. Streptomyces produce a number of bioactive compounds such as antibacterial. This study aimed to investigate the effect of different media and incubation time in increasing the antibacterial activity of marine sponge-associated Streptomyces spp. and characterize antibacterial compounds of marine sponge-associated Streptomyces spp. against pathogenic bacteria. Among the three tested media and some days of incubation times, Streptomyces spp. produce more antibacterial activity when grown using modified molasses medium at 15 days incubation. The ethyl acetate extracts of Dbi28t exhibited a significant inhibitory zone against Extended Spectrum β-Lactamase (ESBL)-producing Escherichia coli, Providencia rettgeri then followed by Proteus mirabilis and Pseudomonas putida and the results were higher than some commercial antibiotics. This study has identified nine antibacterial compounds in Dbi28t using Liquid Chromatography-tandem Mass Spectrometric (LC-MS/MS) analysis, with the most abundance belonging to pumilacidin A, then followed by pumilacidin B, surfactin B, surfactin A, phenazostatin B, chalcomycin B, neopyrrolomycin C, saquayamycin A and saphenamycin. This work provides the first report from a Streptomyces sp. Dbi28t produced pumilacidin, surfactin and other bioactive compounds with the modified molasses medium for optimization of characterization of its antibacterial compounds.
Kemampuan fag litik Listeria dalam menginfeksi Listeria grayi pada matrik udang Retnani Rahmiati; Winiati P Rahayu; Sri Budiarti; Harsi D Kusumaningrum

Publisher : Program Studi Ilmu dan Teknologi Pangan, Universitas Yudharta, Pasuruan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35891/tp.v15i2.5191

Abstract

Shrimp belongs to type of food that oftenly contaminated with Listeria spp. Bacteriophage as an antimicrobial agent represents an attractive way to destroy bacterial contamination on food. This study presents the proof of lytic phage of Listeria (FL03) usage and influenced storage temperature to infect Listeria grayi on food matrix of shrimp. Shrimp samples were inoculated with 1.3 X 107 CFU mL-1 L. grayi (BA03) and then treated with 1.5 X 108 PFU mL-1 lytic phage of Listeria (FL03). The total number of L. grayi was counted at storage times of 0, 1, 24 and 48 hours at room temperature (±28 oC) and cold temperature (±4 oC). The results showed that shrimp treated with phage FL03 showed a decrease in the number of L. grayi compared to the control, namely 0 - 0.15 log10 CFU g-1 at room temperature (±28 oC) and 0.93 - 1.37 log10 CFU g -1 at cold temperatures (±4 oC). Treatment at cold temperatures (±4 oC) can inhibit the growth of L. grayi by 2.01 – 2.98 log10 CFU g-1. The growth inhibition of L. grayi increased further when treatment at cold temperatures (± 4 oC) was combined with FL03 phage treatment, namely to 3.38 – 3.76 log10 CFU g-1. These findings illustrated that phage FL03 could infect and cause lysis of L.grayi on food matrix of shrimp samples.
IN SILICO ANALYSIS ON BINDING ACTION OF TERPENE NATURAL COMPOUNDS FROM APOCYNACEAE FAMILY AGAINST SHV-1 BETA-LACTAMASE FROM Klebsiella pneumoniae A M Sabbah, Sajida; Indri Astuti, Rika; Budiarti, Sri
BIOMA : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2025): October 2025
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/585f2k97

Abstract

Antibiotic resistance is a growing threat to public health, particularly with hospital-acquired infections caused by Klebsiella pneumoniae. The limited treatment options for these infections are further complicated by the emergence of antibiotic-resistant strains due to the overuse of antibiotics. This study investigates the potential of Monoterpene Indole Alkaloids (MIAs) derived from six genera within the Apocynaceae family as inhibitors of the beta-lactamase enzyme (bla-SHV-1), which plays a key role in antibiotic resistance in Klebsiella pneumoniae. Using the PyRx program for molecular docking, we assessed the binding affinity and interaction profiles of various MIAs with bla-SHV-1. Our results identified Paucidisine, (-)-19-Oxoisoeburnamine, and Paucidactine A as the most promising candidates, based on their interaction energies and binding modes. Detailed analysis of ligand-protein interactions, including hydrogen bonds and hydrophobic interactions, revealed that these MIAs exhibit higher binding affinity and structural similarity to the native ligand bla-SHV-1. While these findings are promising, it is important to note that they are based on in silico models. Further in vitro and in vivo studies are required to validate the efficacy and safety of these MIAs as potential therapeutic agents. This research marks a significant step towards developing alternative treatment options for antibiotic-resistant Klebsiella infections, potentially improving patient outcomes and addressing the global challenge of antibiotic resistance. In conclusion, our study offers novel insights into the potential use of MIAs as inhibitors of bla-SHV-1, contributing to the ongoing efforts to combat antibiotic resistance. Future research should focus on validating these in silico results through rigorous experimental and clinical studies, considering the ethical implications of introducing new treatments.
Potret Kebijakan Pengendalian Demam Berdarah Dengue di Kabupaten Indramayu Peranginangin, Henri; Hasim, Hasim; Pramudya, Bambang; Budiarti, Sri
Kesmas Vol. 4, No. 3
Publisher : UI Scholars Hub

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Abstract

Penyakit demam berdarah dengue (DBD) masih menjadi masalah di Kabupaten Indramayu. Penelitian ini bertujuan mengidentifikasi elemen-elemen prioritas kebijakan pengendalian penyakit DBD di Kabupaten Indramayu menurut pakar berdasarkan metode Analytical Hierarchy Process (AHP). Hasil penelitian menunjukkan “strategi utama” pengendalian penyakit DBD di Kabupaten Indramayu adalah peningkatan kesehatan lingkungan permukiman; “aktor utama pengendalian” ialah Pemerintah Kabupaten Indramayu; “faktor utama pengendalian” adalah lingkungan; “tujuan utama pengendalian” ialah Kabupaten Indramayu bebas penyakit DBD; dan “kriteria utama pengendalian” adalah jumlah dan mutu sumber daya manusia. Agar implementasi strategi pengendalian itu efektif maka Pemerintah Kabupaten Indramayu perlu meningkatkan kerja sama lintas program dan sektoral; dukungan teknologi, dana dan sarana pendidikan kesehatan lingkungan; mutu layanan Puskesmas; dan pengembangan tim pengendalian penyakit DBD dari tingkat Kabupaten sampai Desa/Kelurahan. Dengue haemorrhagic fever (DHF) still becomes health problem in Indramayu district. The objective of this research is to identify the priority elements of DHF controlling policy in Indramayu district according to 35 experts using analytical hierarchy process (AHP). The result of the research provides information that the “main strategy” of DHF controlling is the improvement of healthy living environment; the “main actor” is the Government of Indramayu district; the “main factor” is the environment; the “main objective” is zero DHF in Indramayu district; and the “main criteria” is the quantity and quality of human resources. Based on this data, in order to implement the main strategy effectively, the Government of Indramayu district should increase the interprogram and inter-institutional cooperation; provide technological, funding, and facilities of environment health education supports; increase the quality of Puskesmas services and develop the DHF controlling team in all administrative level.
Kemampuan fag litik Listeria dalam menginfeksi Listeria grayi pada matrik udang: The abitity of Listeria lytic phage of infect Listeria grayi in shrimpi matrices Rahmiati, Retnani; Rahayu, Winiati P; Budiarti, Sri; Kusumaningrum, Harsi D
TEKNOLOGI PANGAN : Media Informasi dan Komunikasi Ilmiah Teknologi Pertanian Vol 15 No 2 (2024)
Publisher : Program Studi Ilmu dan Teknologi Pangan, Universitas Yudharta, Pasuruan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35891/tp.v15i2.5191

Abstract

Shrimp belongs to type of food that oftenly contaminated with Listeria spp. Bacteriophage as an antimicrobial agent represents an attractive way to destroy bacterial contamination on food. This study presents the proof of lytic phage of Listeria (FL03) usage and influenced storage temperature to infect Listeria grayi on food matrix of shrimp. Shrimp samples were inoculated with 1.3 X 107 CFU mL-1 L. grayi (BA03) and then treated with 1.5 X 108 PFU mL-1 lytic phage of Listeria (FL03). The total number of L. grayi was counted at storage times of 0, 1, 24 and 48 hours at room temperature (±28 oC) and cold temperature (±4 oC). The results showed that shrimp treated with phage FL03 showed a decrease in the number of L. grayi compared to the control, namely 0 - 0.15 log10 CFU g-1 at room temperature (±28 oC) and 0.93 - 1.37 log10 CFU g -1 at cold temperatures (±4 oC). Treatment at cold temperatures (±4 oC) can inhibit the growth of L. grayi by 2.01 – 2.98 log10 CFU g-1. The growth inhibition of L. grayi increased further when treatment at cold temperatures (± 4 oC) was combined with FL03 phage treatment, namely to 3.38 – 3.76 log10 CFU g-1. These findings illustrated that phage FL03 could infect and cause lysis of L.grayi on food matrix of shrimp samples.
Co-Authors A M Sabbah, Sajida ACHMAD DINOTO ALEX HARTANA ALEX HARTANA Alex Hartana Alex Hartana ALEX HARTANA ANIK KUSMIATUN Asmadi Asmadi Asmendri Asmendri Bambang Pramudya Bambang Pramudya Bambang Suryobroto Budi Wirawan Damaini, Amaya Andri Demina Desniar - - DEWI SARTIKA DITA, SASMIATI FARACH Efendi, Firda Sri Elda Irma Jeanne Joice Elda Irma Jeanne Joice Kawulur Fahrurroji Fitriandini, Risa Getriani, Zerina Harsi D. Kusumaningrum Hasim Hasim Hasim Hasim Hendri Susanto Hendri Susanto Henri Peranginangin Henri Peranginangin Heru Irianto I Wayan T. Wibawan I wayan Teguh Wibawan Iman Rusmana Jepri Agung Priyanto, Jepri Agung Katupayan, Sisilia Lilis Nuraida Machmud Arifin Raimadoya MAGGY T. SUHARTONO Maggy T. Suhartono Maggy Thenawidjaja Suhartono Meilany Ariati Dewi MIRNAWATI SUDARWANTO NISA RACHMANIA MUBARIK Niza, Yesita Roza Normalia, Normalia Nuraeni, Siti Panesya Oktavia, Wiwik Oktonuah, Elvandro Rahmiati, Retnani Rahminiwati, Min Reflinda Retnani Rahmiati Rifky, Muhammad Rika Indri Astuti RONI RIDWAN1 Saputri, Sindi Sari, Mila Supriatno Supriatno - Sutandi, Michellia Salmaa Ashilla TATI NURHAYATI TETRI WIDIYANI Teuku Fadrial Karmil Wendry Setiyadi Putranto Wendy Setiyadi Putranto Wibowo, Muhammad Arya Winiati P Rahayu Winiati P Rahayu YANTYATI WIDYASTUTI1 YULIN LESTARI Zainatul Hayati Zinatul Hayati Zulhaerati Zulhaerati