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All Journal Buletin Veteriner Udayana Buletin Udayana Mengabdi Jurnal Veteriner Indonesia Medicus Veterinus Media Kedokteran Hewan
Ida Bagus Kade Suardana
Laboratorium Virologi Veteriner, Fakultas Kedokteran Hewan Universitas Udayana. JL. Sudirman, Sanglah, Denpasar, Bali, Indonesia 80234.
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Dog Demography And Level Of Knowledge Against Rabies In Positive And Negative Case Of Rabies Areas In Mendoyo-Jembrana, Bali, Indonesia (DEMOGRAFI ANJING DAN TINGKAT PENGETAHUAN MASYARAKAT TERHADAP RABIES DI DAERAH DENGAN KASUS RABIES POSITIF DAN NEGATIF Kadek Karang Agustina; Putu Sasmitha Devi Cahyani; Ida Bagus Kade Suardana
Jurnal Veteriner Vol 18 No 4 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (121.024 KB) | DOI: 10.19087/jveteriner.2017.18.4.642

Abstract

This research aims were to compare the demography of dogs and knowledge profile of dog owners against rabies in Mendoyo Sub-District, Jembarana Bali. This research used observational method, conducted from May to June 2016 in Mendoyo sub-district of Jembrana. Two Banjars were selected by the rabies case report in dogs (YehEmbangKangin village) and two other without case (MendoyoDanginTukad village). The data collected by interviewing the community and direct assessment of free-roaming dogs in the areas. A total of 200 household samples were taken, 50 respondents of each sampling area. This research resulted that the ratio of man-dog in Mendoyo was 4.5:1 and the ratio of male and female dogs was 1.8:1. As many as 64.8% of dog’s age were more than 12 month old, 28% were 4 to 12 month old and 8% were less than 4 month old. In the sampling areas, 19.4% of dogs were sterilized, which were 91.18% males and 8.82% females. Coverage of rabies vaccination in owned dogs was 82.3%. Dog’s petting method by people in Mendoyo was 69.71% free-roaming while 30.29% tied or caged. On free-roaming dog assessment, this study found 52 free-roaming dogs on the streets, 65.38% males and 34.62% females, only 30.8% of those used vaccination collar marker, Based on the body condition score, 76.9% of free-roaming dogs have good conditions. Of the total 200 households surveyed, there was a high level of knowledge about the danger of rabies and the importance of vaccination, but only a few people (<35%) received consulting about rabies, understood how to prevent rabies infection and the regulations about dog petting management. In positive area, we found there are still many people who have the habit of disposing dog. We conclude that there is needed to improve knowledge and attitude of the community about the prevention and risk factor of rabies.
Pelacakan Kasus Flu Burung pada Ayam dengan Reverse Transcriptase Polymerase Chain Reaction* (DETECTION OF AVIAN INFLUENZA IN CHICKENS BY REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION) Gusti Ayu Yuniati Kencana; I Gusti Ngurah Kade Mahardika; Ida Bagus Kade Suardana; I Nyoman Mantik Astawa; Ni Made Krisna Dewi; Gusti Ngurah Narendra Putra
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (131.81 KB)

Abstract

Avian Influenza (AI) or Bird Flu is a fatal zoonotic disease caused by highly pathogenic avian influenza(HPAI) virus of H5N1 sub-type. The disease is still endemic in Indonesia. This study was conducted toinvestigate AI cases in chickens in Bali. Virus isolation was performed in 9 day-old embryonated chickeneggs, and then followed by serologic testing by haemaglutination (HA) and Haemaglutination Inhibition(HI) assay using standard microtiter procedure. All of the samples were further tested with reversetrancriptasepolymerase chain reaction (RT-PCR). All work has been done in the Biomedical and MolecularBiology Laboratory, Faculty of Veterinary Medicine, Udayana University, Denpasar, during the period2009-2011. A total of ten samples were examined A total of ten chicken samples consisting of 6 fieldsamples and 4 meat samples have been confirmed to be AIV H5N1. All field cases showed clinical signsand gross pathology that were typical to the infection of avian influenza. The result indicates that AI casesare still prevalent among chickens in Bali.
Respons Imun Mencit terhadap Vaksin DNA Virus Demam Babi Afrika A224L dan A276R dengan Enkapsulasi Lipofektamin, Kolesterol dan Polimer Sylvia Kamil; I Gusti Ngurah Kade Mahardika; Ida Bagus Kade Suardana
Jurnal Veteriner Vol 25 No 1 (2024)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2024.25.1.132

Abstract

Vaksin terhadap penyakit Demam Babi Afrika (African Swine Fever/ASF) sangat diperlukan untuk mengurangi kerugian peternak babi di seluruh dunia. Penelitian ini bertujuan memaparkan pengujian vaksin DNA-ASF dengan gen A224L dan A276R pada mencit dengan adjuvan polimer, lipofektamin dan kolesterol. Setiap mencit divaksinasi secara intramuskuler dengan 25 ?g plasmid yang dienkapsulasi dengan adjuvan tersebut. Serum mencit dikumpulkan pada minggu ke-1, 2, 3 dan 4. Antibodi terhadap A224L dan A276R dideteksi dengan uji ELISA menggunakan peptida sintetik A224L dan A276R sebagai antigen. Nilai optical density (OD) dianalisis secara statistika menggunakan uji sidik ragam dengan aplikasi SPSS Versi 25. Hasil penelitian menujukkan nilai OD serum mencit yang diberikan vaksin DNA ASF-A224L dan A276R dengan adjuvan polimer (0,149), lipofektamin (0,080) dan kolesterol (0,058), serta mencit kontrol (0,020) berbeda sangat nyata (p=0,000), sementara waktu pengambilan serum tidak berpengaruh nyata (p=0,517). Simpulannya adalah nilai OD ELISA serum mencit yang diberikan vaksin DNA ASF-A224L dan A276R antarperlakuan adjuvan polimer, lipofektamin, kolesterol, dan kontrol secara statistika berbeda sangat nyata (p=0,000), sedangkan waktu pengambilan serum secara statistika tidak berpengaruh nyata (p=0,517) terhadap nilai OD ELISA serum mencit yang diberikan vaksin DNA ASF-A224L dan A276R. Kajian lebih lanjut diperlukan untuk memperbaiki protokol pencampuran adjuvan dengan plasmid menggunakan kuantitas lebih banyak.
Indonesian Newcastle Disease Virus Field Isolate Reduces c-Jun Expression in Rat Mammary Cancer Models Marson, Fransiska Gratia Sonita; Sewoyo, Palagan Senopati; Astawa, I Nyoman Mantik; Adi, Anak Agung Ayu Mirah; Suardana, Ida Bagus Kade; Winaya, Ida Bagus Oka; Berata, I Ketut
Media Kedokteran Hewan Vol. 36 No. 1 (2025): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v36i1.2025.13-20

Abstract

c-Jun is often found to be overexpressed in various cancers, so this gene might be a target for cancer therapy. Newcastle disease virus (NDV) is recognized for its oncolytic properties and potential as a cancer virotherapy agent, with various mechanisms reported to trigger cancer cell death. This study aimed to assess the c-Jun expression in rat mammary cancer models. Rat mammary cancer models were categorized into two treatment groups: the control group (C) and the virotherapy group (V). Group C was administered with 0.5 cc of sterile PBS, while group V received 7 log 2 HAU per 0.5 cc of the Indonesian NDV field isolate Tabanan-1/ARP/2017 intratumorally. The treatment was carried out for four days in a row. Two weeks after treatment, all rats were humanely euthanized, and mammary cancer tissues were excised for further examination. Mammary cancer tissues were examined histopathologically and analyzed using immunohistochemistry to determine intranuclear c-Jun expression, quantified by the H-Score. The results demonstrated that NDV significantly reduced c-Jun expression. It can be inferred that NDV Tabanan-1/ARP/2017 holds potential as a mammary cancer therapy agent by reducing c-Jun expression. This finding is considered novel, as there have been no previous reports of decreased c-Jun expression following virotherapy with NDV.
Co-Authors Akbar, Saiful Anak Agung Ayu Mirah Adi Anak Agung Sagung Kendran Anindya Novitasari Azizah, Hidayatul Brigita Galilea Adu Cahyani, Ni Luh Risna Desak Wiga Puspita Dewi devinda yuniastika Dewi, Desak Made Wiga Puspita Dewi, Putu Ayu Purbani Novia Faiziah - Gusti Ayu Mayani Kristina Dewi Gusti Ayu Yuniati Kencana Gusti Ngurah Narendra Putra Hanesty Jantiko, Hanesty I Gusti Agung Ayu Suartini I Gusti Ngurah Kade Mahardika I Gusti Ngurah Kade Mahardika I Gusti Ngurah Narendra Putra1, I Ketut Berata I Made Kardena I Made Sukada I Nengah Sudarmayasa I NYOMAN MANTIK ASTAWA I Nyoman Suartha I Putu Sampurna I Putu Sudiarta I Wayan Sudarmayasa I Wayan Sudira I wayan Teguh Wibawan I Wayan Wirata I.A.P. Apsari Ida Ayu Pasti Apsari Ida Ayu Sri Chandra Dewi Ida Bagus Komang Ardana Ida Bagus Oka Winaya Ida Bagus Windia Adnyana Jayanata, I Made Kadek Karang Agustina Ketut Tono Pasek Gelgel Lijung, Derfina Luh Putu Agustini Marson, Fransiska Gratia Sonita Messy Saputri Boru Sembiring Ni Made Krisna Dewi Ni Made Ritha Krisna Dewi Norawigaswari, Nengah Desy Oktryna Hodesi Sibarani Pawestri, Mega Mijil Pradnyandika, I Putu Krisna Ardhia Prasatya, I Gde Made Abdi Pratiwi, Ni Made Diah Kusuma Putu Sasmitha Devi Cahyani Sewoyo, Palagan Senopati Sri Kayati Widyastuti Suriana, Ni Komang Valerie Sylvia Kamil Tjokorda Sari Nindhia TRI KOMALA SARI Widyaningsih, Putu Oka Yulianti, Vanesya