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All Journal HAYATI Journal of Biosciences JURNAL KIMIA SAINS DAN APLIKASI Jurnal Kedokteran dan Kesehatan : Publikasi Ilmiah Fakultas Kedokteran Universitas Sriwijaya Global Medical and Health Communication Jurnal Kebidanan Jurnal Kebidanan Dan Kesehatan Tradisional Majalah Kedokteran Sriwijaya JPSriwijaya Biomedical Journal of Indonesia Padjadjaran Journal of Dentistry Jurnal Farmasi Sains dan Komunitas Universa Medicina Jurnal Fitofarmaka Indonesia Jurnal Kedokteran dan Kesehatan Syifa' MEDIKA: Jurnal Kedokteran dan Kesehatan Midwifery Journal: Jurnal Kebidanan UM. Mataram JSFK (Jurnal Sains Farmasi & Klinis) Manuju : Malahayati Nursing Journal Logista: Jurnal Ilmiah Pengabdian Kepada Masyarakat Acta Biochimica Indonesiana SRIWIJAYA JOURNAL OF MEDICINE Molekul: Jurnal Ilmiah Kimia Jurnal Ilmu Kefarmasian Indonesia Majalah Kedokteran Andalas YARSI Medical Journal Majalah Patologi Indonesia Jurnal Biomedika dan Kesehatan Jurnal Teknik Kimia Jurnal Sains Kebidanan Bioscientia Medicina : Journal of Biomedicine and Translational Research Cermin Dunia Kedokteran Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Conferences of Medical Sciences Dies Natalis Kreativitas Pada Pengabdian Masyarakat (Krepa) Molekul: Jurnal Ilmiah Kimia Borneo Journal of Pharmacy Musyawarah: Jurnal Pengabdian Masyarakat Cermin Dunia Kedokteran Fajar: Media Komunikasi dan Informasi Pengabdian Kepada Masyarakat Folia Medica Indonesiana Global Medical and Health Communication
Subandrate
Bagian Biokimia Dan Kimia Medik, Fakultas Kedokteran, Universitas Sriwijaya, Palembang, Indonesia
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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Inhibitory Effect of Ethanol Extract of Cherry Mistletoe Leaves (Dendrophthoe pentandra (L.) Miq) on Xanthine Oxidase Enzyme Hana Daini Sabathania; Subandrate Subandrate; Sadakata Sinulingga; Safyudin Safyudin; Liniyanti D Oswari; Fatmawati Fatmawati
Jurnal Farmasi Sains dan Komunitas (Journal of Pharmaceutical Sciences and Community) Vol 22, No 2 (2025)
Publisher : Sanata Dharma University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24071/jpsc.008252

Abstract

One plant that can be used as an alternative treatment for hyperuricemia is the cherry plant. The usefulness of these plants is due to the secondary metabolite compounds that can inhibit the enzyme xanthine oxidase. Cherry mistletoe is thought to have the same flavonoids, saponins, alkaloids, terpenoids, and tannins as the cherry plant, which can act as inhibitors. The aim of the study was to determine the inhibitory effect of an ethanol extract of cherry mistletoe leaves on the xanthine oxidase enzyme. This study was conducted as  an in vitro experimental study. Cherry mistletoe leaves were extracted with 96% ethanol. The extract was done with phytochemical screening, followed by an inhibition test on the xanthine oxidase enzyme using a spectrophotometer at a wavelength of 400 nm. Allopurinol was a positive control. The ethanol extract of cherry mistletoe leaves contains flavonoids, saponins, alkaloids, terpenoids, and tannins. The IC50 value of the ethanol extract of cherry mistletoe leaves was 23.44 mg/L. The ethanol extract of cherry mistletoe leaves has an inhibitory effect on the xanthine oxidase enzyme with moderate activity. Accordingly, the cherry mistletoe leaf extract can be considered as an alternative inhibitor of the xanthine oxidase enzyme in the control of hyperuricemia.
Hubungan Antara Karakter Klinikopatologik dengan Grup Prognostik WHO pada Pasien Gastrointestinal Stromal Tumor di RSUP Dr. Mohammad Hoesin, Palembang Nur Azizah; Suly Auline Rusminan; Subandrate
Majalah Patologi Indonesia Vol. 30 No. 1, Januari 2021
Publisher : Perhimpunan Dokter Spesialis Patologi Anatomik Indonesia (PDSPA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55816/mpi.v30i1.463

Abstract

BackgroundGastrointestinal stromal tumors are the most common mesenchymal tumors in the gastrointestinal tract. Based on tumor size andmitotic index, WHO classified GIST into three prognostic groups which were benign, uncertain malignant potential, and malignant.Some studies revealed that there was variation in association between age, gender, tumor location, histopathologic type and prognosisof GIST patients. This study aimed to identify and analyze the association between clinicopathologic characteristics and WHOprognostic group in GIST patients at RSUP Dr. Mohammad Hoesin Palembang.MethodsThis study used 30 medical records of GIST patients during 01 January 2014 to 27 December 2018 in the Department of AnatomicPathology RSUP Dr. Mohammad Hoesin. Clinicopathologic characteristics and WHO prognostic group data were collected. Theassociation between clinicopathologic characteristics (age, gender, tumor location, histopathologic type) and WHO prognostic groupwere analyzed by chi-square test.ResultsFrom 30 subjects, 40% of the patients aged 52-58 years.There was no gender predilection. A total of 33.3% of the GISTs were locatedin the small intestine. Majority of histopathologic type was spindle cell type (93.3%). Bivariate analysis showed there were no significantassociation between clinicopathologic characteristics: age (p=0.811), gender (p=0.682), tumor location (p=1.000), histopathologic type(p=0.469) and WHO prognostic group.ConclusionThere was no significant association between clinicopathologic characteristics and WHO prognostic group in GIST patients at RSUPDr. Mohammad Hoesin Palembang. We suggest doing a cohort study about association between clinicopathologic characteristics andsurvival rate in GIST patients.
Kepuasan Pasien terhadap Persalinan Operasi Seksio Sesaria dengan Protokol Enhanced Recovery After Cesarean Surgery (ERACS) di Rumah Sakit Tingkat Sekunder di Indonesia Eka Handayani Oktharina; Alya Zafira; Subandrate Subandrate; Raissa Nurwany; Medina Athia
Malahayati Nursing Journal Vol 8, No 8 (2026): Volume 8 Nomor 8 (2026)
Publisher : Universitas Malahayati Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/mnj.v8i8.26788

Abstract

ABSTRACT The increasing rate of cesarean section has been associated with a higher incidence of postoperative complications, prolonged recovery, and reduced patient comfort. Enhanced Recovery After Cesarean Surgery (ERACS) was introduced as an evidence-based perioperative care pathway to accelerate postoperative recovery while improving patient-centered outcomes. This descriptive observational study was conducted from September to November 2024 at a secondary-level hospital in Indonesia and included all patients who underwent cesarean section using the ERACS protocol during the study period. Primary data were collected through structured interviews using a standardized questionnaire, while secondary data were obtained from medical records. A total sampling technique was employed, resulting in 203 eligible participants. Data were analyzed using descriptive statistics. Most participants were aged 20–35 years, multiparous, at term gestation, and had a hospital stay of two days. Overall, patient satisfaction with ERACS was high across all evaluated domains. Very high satisfaction was reported for preoperative, intraoperative, and postoperative hospital services. Most patients also expressed high satisfaction with the adequacy of information provided, postoperative nausea and vomiting control, pain management, early mobilization, timely urinary catheter removal, and appropriate initiation of oral intake, with the highest level of satisfaction observed for early oral intake. These findings indicate that cesarean section performed using the ERACS protocol provides high patient satisfaction throughout the perioperative and postoperative periods, supporting ERACS as an effective, evidence-based, and patient-centered approach to enhancing postoperative recovery and improving the overall patient experience following cesarean delivery. Keywords: Cesarean Section, ERACS, Patient Satisfaction, Enhanced Recovery, Obstetric Surgery.  ABSTRAK Peningkatan angka persalinan dengan operasi seksio sesaria berkaitan dengan meningkatnya risiko komplikasi pascaoperasi, pemulihan yang lebih lama, serta berkurangnya kenyamanan pasien. Enhanced Recovery After Cesarean Surgery (ERACS) dikembangkan sebagai pendekatan pelayanan perioperatif berbasis bukti yang bertujuan mempercepat enhanced recovery sekaligus meningkatkan luaran yang berpusat pada pasien. Penelitian ini menggunakan desain observasional deskriptif yang dilaksanakan pada September hingga November 2024 di salah satu rumah sakit tingkat sekunder di Indonesia. Sampel penelitian meliputi seluruh pasien yang menjalani persalinan dengan operasi seksio sesaria menggunakan protokol ERACS selama periode penelitian. Data primer diperoleh melalui wawancara terstruktur menggunakan kuesioner baku, sedangkan data sekunder diperoleh dari rekam medis. Teknik total sampling menghasilkan 203 responden yang memenuhi kriteria penelitian, dan data dianalisis secara deskriptif. Sebagian besar responden berusia 20–35 tahun, multipara, berada pada usia kehamilan aterm, serta menjalani lama rawat inap selama dua hari. Secara keseluruhan, tingkat kepuasan pasien terhadap penerapan ERACS tergolong tinggi pada seluruh aspek yang dievaluasi. Tingkat kepuasan yang sangat tinggi ditemukan pada pelayanan praoperatif, intraoperatif, dan pascaoperatif. Sebagian besar pasien juga menyatakan sangat puas terhadap kecukupan informasi yang diberikan, pengendalian mual dan muntah pascaoperasi, pengelolaan nyeri, pelaksanaan mobilisasi dini, waktu pelepasan kateter urin, serta ketepatan pemberian asupan oral, dengan tingkat kepuasan tertinggi terdapat pada waktu dimulainya pemberian asupan oral. Temuan ini menunjukkan bahwa penerapan protokol ERACS mampu memberikan tingkat kepuasan pasien yang tinggi pada seluruh komponen pelayanan perioperatif dan pascaoperatif, sehingga mendukung ERACS sebagai pendekatan pelayanan yang efektif, berbasis bukti, dan berpusat pada pasien dalam mempercepat pemulihan serta meningkatkan pengalaman pasien setelah persalinan dengan seksio sesaria.  Kata Kunci: Seksio Sesaria, ERACS, Kepuasan Pasien, Enhanced Recovery, Operasi Obstetri.
Crosstalk Apoptosis-Autofagi pada Kanker Serviks: Peran BAX, BCL-2, dan Cleaved Caspase-3 Diah Komala Sari; Mgs Irsan Saleh; Subandrate Subandrate; Shaum Shiyan
Jurnal Biomedika dan Kesehatan Vol 9 No 2 (2026)
Publisher : Fakultas Kedokteran Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/JBiomedKes.2026.v9.207-218

Abstract

Cervical cancer remains a major cause of cancer-related morbidity and mortality among women worldwide. In addition to persistent human papillomavirus (HPV) infection, dysregulation of cell survival and cell death mechanisms contributes to cervical cancer development and progression. Apoptosis and autophagy are two important biological processes involved in regulating cancer cell survival and death; however, the interaction between these processes remains complex and context-dependent. This review aimed to examine the crosstalk between apoptosis and autophagy in cervical cancer, with a particular focus on the roles of BAX, BCL-2, and cleaved Caspase-3. A narrative literature review was conducted using PubMed to identify publications related to apoptosis–autophagy crosstalk in cervical cancer, with particular attention to studies discussing BAX, BCL-2, cleaved Caspase-3, and selected autophagy-related regulators published between 2020 and 2025. Articles were selected based on relevance to the review topic and were synthesized descriptively to provide an overview of current evidence. The reviewed studies indicated that increased apoptotic activity was frequently associated with BAX upregulation, BCL-2 downregulation, and activation of cleaved Caspase-3. In contrast, autophagy exhibited a context-dependent role and functioned either as a mechanism of cancer cell death or as a protective process that promotes cancer cell survival. The interaction between BCL-2 and Beclin1, together with the PI3K/Akt/mTOR signaling pathway, emerged as important components of apoptosis–autophagy crosstalk. The conclusions was the balance between apoptosis and autophagy appears to influence cervical cancer cell responses to therapeutic interventions. BAX, BCL-2, and cleaved Caspase-3 may provide useful molecular information for future investigations of cervical cancer biology and therapeutic responses
Clinical Factors of Linezolid-associated Toxic Optic Neuropathy in Drug-resistant Tuberculosis Patients Dwi Indira Setyorini; Zen Ahmad; Zen Hafy; Devi Azri Wahyuni; Subandrate Subandrate; Ella Amalia
Global Medical & Health Communication (GMHC) Vol. 14 No. 2 (2026): Accredited Sinta 2
Publisher : UPT Publikasi Ilmiah Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/gmhc.v14i2.9676

Abstract

Linezolid remains a key component in drug-resistant tuberculosis (DR-TB) regimens but is associated with toxic optic neuropathy (TON). Although mitochondrial dysfunction has been implicated in linezolid-induced neurotoxicity, the contribution of mitochondrial DNA (mtDNA) variants remains uncertain, with limited data from Indonesian populations. This study aimed to evaluate clinical risk factors and mitochondrial DNA polymorphisms associated with linezolid-related TON in DR-TB patients. This cross-sectional analytical study was conducted at Dr. Mohammad Hoesin Hospital in Palembang from January to March 2025 and involved 51 DR-TB patients receiving linezolid therapy, selected through consecutive sampling. Demographic and clinical data were collected, and mtDNA polymorphisms (G3010A and A2706G) were examined using PCR-RFLP, chosen based on previous studies linking these variants to linezolid toxicity in Asian and African populations. TON was identified in 22 of 51 patients (43.1%) based on standardized ophthalmological criteria. Diabetes mellitus (PR=2.078, 95% CI=1.191–3.624, p=0.03) and treatment regimen (PR=12.469, 95% CI=1.821–85.398, p=0.00) demonstrated statistically significant associations with TON, whereas age (p=0.635), sex (PR=0.713, 95% CI=0.384–1.322, p=0.29), HIV status (PR=1.600, 95% CI=0.672–3.809, p=0.40), and DR-TB type (PR=1.438, 95% CI=0.735–2.812, p=0.28) were not associated. All subjects had a wild-type genotype for G3010A and a mutant-type genotype for A2706G, precluding any association analysis for these variants. In conclusion, diabetes mellitus and individual treatment regimens were associated with linezolid-induced TON in this study. In contrast, the investigated mtDNA polymorphisms (G3010A and A2706G) showed no variability; thus, no relationship could be evaluated. Larger prospective studies with expanded genetic markers and multivariate adjustment are warranted to characterize susceptibility factors for linezolid-associated TON better.
Maternal Body Mass Index as a Determinant of Breast Milk Fat Content in Young Mothers Subandrate Subandrate; Vera Tanzey; Liniyanti D Oswari; Fatmawati Fatmawati; Safyudin Safyudin; Medina Athiah
Jurnal Kebidanan dan Kesehatan Tradisional Jurnal Kebidanan dan Kesehatan Tradisional Volume 11 Number 2 Year 2026
Publisher : Poltekkes Kemenkes Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37341/jkkt.v11i2.683

Abstract

Background: Fat is a major macronutrient in breast milk, and its concentration varies according to multiple factors, including stage of lactation, time of collection, milk fraction, feeding duration, maternal diet, and maternal body mass index (BMI). Alterations in breast milk fat composition may influence infant growth patterns and long-term metabolic health. This study aimed to examine the association between maternal BMI and the fat content of expressed breast milk. Methods: An analytical observational study with a cross-sectional design was conducted at the Medical Basic Chemistry Laboratory, Faculty of Medicine, Universitas Sriwijaya. Breast milk samples were obtained from 63 breastfeeding mothers in Palembang City, comprising 30 mothers with normal BMI and 33 mothers with obese BMI. Breast milk fat concentration was measured using the glycerol-3-phosphate oxidase–phenol aminophenazone (GPO-PAP) method. Data were analyzed using an independent t-test. Results: The mean fat concentration of breast milk from mothers with normal BMI was 3786.7±743.2 mg/dL, whereas that from mothers with obese BMI was 4339.2±958.0 mg/dL. A statistically significant difference in fat concentration was observed between the two groups (p=0.014). Conclusion: Maternal BMI is significantly associated with the fat content of expressed breast milk, with higher fat concentrations observed in mothers with obese BMI. These findings imply that maternal nutritional status should be considered in lactation counseling and infant feeding practices, particularly to optimize infant growth and metabolic outcomes.
Biomolecular and bioinformatics characterization of salivary matrix metalloproteinase-9 as oral cancer potential targeted therapy Dewi, Siti Rusdiana Puspa; Saleh, Muhammad Irsan; Murti, Krisna; Parisa, Nita; Rosdah, Ayeshah Augusta; Subandrate, Subandrate; Kumar, Arun
Padjadjaran Journal of Dentistry Vol 38, No 2 (2026): July 2026
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol38no2.70464

Abstract

Introduction: Oral cancer is the 11th most common cancer worldwide. Previous studies have reported that saliva contains biomarkers for oral cancer. MMP-9, found in saliva, is a marker that indicates and facilitates tumor development. However, comprehensive biomolecular and bioinformatics characterization of salivary MMP-9 remains limited. This study aimed to analyze the biomolecular and bioinformatics characteristics of salivary MMP-9 as an oral cancer biomarker and a potential target for therapy because it plays an important role in tumor development and management. Methods: The features of MMP-9 were obtained from the NCBI database (www.ncbi.nlm.nih.gov). Protein analyses were performed using Protparam site, PROTSCALE, TMM application, PEPTIDE CUTTER, NETNGLYC and TARGETTP. The protein model and structure of MMP-9 were evaluated using SWISSMODEL. Results: Based on the study, it showed that MMP-9 is located on 20q13.12. It is involved in extracellular matrix breakdown, and this enzyme degrades type IV and V collagens. Based on the instability index (II), the protein was found to be unstable. The hydrophobicity range of the MMP-9 protein was 0–7654. Among the 20 amino acids analyzed, the hydrophobicity of arginine was -4.500, while isoleucine was 4.500. Transmembrane helix analysis indicated the MMP-9 protein isoform is predominantly located outside of the cell. The predicted N-glycosylation site was at the 28th amino acid. The structural model analysis indicated that the protein model was of good quality. Conclusion: Biomolecular and bioinformatics characterization of salivary MMP-9 demonstrates strong potential as an oral cancer biomarker. The biomolecular and bioinformatics characteristics identified in this study support the potential utility of MMP-9 as an oral cancer biomarker and provide a foundation for future studies investigating its therapeutic relevance.
Co-Authors Adiyatma Putra Mahardika Adma, Annes Claudia Ahmad, Zen Aldri Frinaldi Alexander Theo Yuda Salean Alya Zafira Anggraini Tiara Septiyana Gunawan Aninditia, Fahira Aninditia, Fahira Annes C. Adma Aprilia Putri Arifin, Mutmainah Arrafi, Muhammad Hafizh Athiah, Medina Aulia Firdha Tariza Ayeshah A Rosdah Az Zahra, Fatihah Bebbi Arisya Kesumaputri Danang Bagus Untoro Dazakiah Dazakiah Devi Azri Wahyuni Diah Komala Sari Dita Tri Ramadianti Dwi Indira Setyorini Dwi Indira Setyorini Dwi Indira Setyorini Dwi Indira Setyorini Dwirini Retno Gunarti Dzakiyah, Dzakiyah Dzakiyah, Dzakiyah Eka Febri Zulissetiana Eka Handayani Oktharina Eka Handayani Oktharina Ella Amalia Ella Amalia Ella Amalia, Ella Ellla Amalia Erial Bahar Erial Bahar Eva Syahriana Eva Syahriana Evlin Kohar Ezra Hans Soputra Fadel Fikri Suharto Fadel Fikri Suharto Fahira Aninditia Fahira Aninditia Faisal, Mia EPA Fatihah Az Zahra Fatmawati Fatmawati Fatmawati Fatmawati Fatmawati Fatmawati, Fatmawati Fifa Argentina Firdaus, Mutia Muthmainnah Galuh Anggraini Hana Daini Sabathania Hariyadi, Kusumo Hayati, Lusiya Hayati, Lusiya Hermansyah Hermansyah Hermansyah Hestiningsih, Tyas Hutabarat, Yosafat Beltsazar Iche Andriyani Liberty Iche Andriyani Liberty, Iche Andriyani Intan Rahma Dewi Irfannuddin Irfannuddin Irfannudin Irfannudin Irsan Saleh Izzah, Puspita Nurul Kohar, Evlin Kohar, Evlin Krisna Murti Kumar, Arun Kusumo Hariyadi Kusumo Hariyadi Kusumo Hariyadi Kusumo Haryadi Liniyanti D Oswari Liniyanti D Oswari Liniyanti D. Oswari Liniyanti D. Oswari Lusia Hayati M Fakhri Altiyan M Fitra Romadhon M. Dias A. Monanda M. Hafizh Arrafi M. Hafizh Arrafi M. Nadhif Prasetyo M. Nadhif Prasetyo Maharani Puspita Sari HS Mariana Mariana Mario Saputra Masayu Farah Diba Masayu Farah Diba Mediarty Mediarty Mediarty Mediarty Medina Athia Medina Athiah Melina Indah Sari Mgs Irsan Saleh Mgs. Irsan Saleh Mgs. Irsan Saleh Mia EPA Faisal Mia Esta Poetri Afdal Faisal, Mia Esta Poetri Afdal Mohamad Sadikin MOHAMAD SADIKIN Mohamad Sadikin Monanda, M Dias Athallah Monanda, M. Dias Athallah Muhammad Irsan Saleh Muniaty Sulam Ng Muthmainnah Arifin Mutiara Budi Azhar Nadia Permata Sari Naomi W Tioline Natasha, Amanda Ng, Muniaty Sulam Nita Parisa Nudhar, Lathifah Nur Azizah Nur Azizah Nurul Windi Anggraini Nurul Windi Anggraini, Nurul Windi Oktalisa, Wenni Oktharina, Eka Handayani Oswari, Liniyanti Oswari, Liniyanti D Pearly Laurencia Permono, Trijoso Putriana Fuji Safitri Raafqi Ranasasmita Raissa Nurwany Rasyid, Riana Sari Puspita Ray Suga Aulia Ray Suga Aulia Sentani Riana Sari Puspita Rasyid Rini Yana Rini Yana Rini Yana Rini Yana Rizki Andini Nawawi Romadhon, M Fitra Sadakata Sinulingga Sadakata Sinulingga Sadakata Sinulingga Sadakata Sinulingga Sadakata Sinulingga Safyudin Safyudin Safyudin Safyudin Safyudin Safyudin Safyudin Safyudin Safyudin Safyudin Safyudin Safyudin Safyudin Saleh, Mgs. M. Irsan Saleh, Muhammad Irsan Salni Salni Salni Salni Salni Salni Salni Santosa, Dorothy Eunike Setyorini, Dwi Indira Shaum Shiyan Siti Rusdiana Puspa Dewi Soputra, Ezra Hans Soputra, Ezra Hans Sri Hartini Harianja Sri Nita Sri Nita Sri Nita, Sri SRI RAHAYU Sri Wahyuni Suly Auline Rusminan Susilawati Susilawati Susilawati, Susilawati Tri Suciati Trijoso Permono Trijoso Permono Triwani Triwani Untoro, Danang Bagus Untoro, Danang Bagus Vera Tanzey Yana, Rini Yudianita Kesuma, Yudianita Zen Ahmad Zen Hafy Zen Hafy