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All Journal HAYATI Journal of Biosciences JURNAL KIMIA SAINS DAN APLIKASI Jurnal Kedokteran dan Kesehatan : Publikasi Ilmiah Fakultas Kedokteran Universitas Sriwijaya Folia Medica Indonesiana Global Medical and Health Communication Jurnal Kebidanan Majalah Kedokteran Sriwijaya JPSriwijaya Biomedical Journal of Indonesia Jurnal Farmasi Sains dan Komunitas Universa Medicina Jurnal Fitofarmaka Indonesia Jurnal Kedokteran dan Kesehatan Syifa' MEDIKA: Jurnal Kedokteran dan Kesehatan Midwifery Journal: Jurnal Kebidanan UM. Mataram JSFK (Jurnal Sains Farmasi & Klinis) Logista: Jurnal Ilmiah Pengabdian Kepada Masyarakat Acta Biochimica Indonesiana SRIWIJAYA JOURNAL OF MEDICINE Molekul: Jurnal Ilmiah Kimia Jurnal Ilmu Kefarmasian Indonesia Majalah Kedokteran Andalas YARSI Medical Journal Majalah Patologi Indonesia Bioscientia Medicina : Journal of Biomedicine and Translational Research Jurnal Teknik Kimia Jurnal Sains Kebidanan Bioscientia Medicina : Journal of Biomedicine and Translational Research Cermin Dunia Kedokteran Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Conferences of Medical Sciences Dies Natalis Kreativitas Pada Pengabdian Masyarakat (Krepa) Molekul: Jurnal Ilmiah Kimia Borneo Journal of Pharmacy Musyawarah: Jurnal Pengabdian Masyarakat Cermin Dunia Kedokteran Fajar: Media Komunikasi dan Informasi Pengabdian Kepada Masyarakat

Subandrate

Bagian Biokimia Dan Kimia Medik, Fakultas Kedokteran, Universitas Sriwijaya, Palembang, Indonesia

Author-ID : 386992

Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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EDUKASI KANDUNGAN ASI DAN PEMBERIAN ASI EKSKLUSIF PADA BAYI Ellla Amalia; Subandrate Subandrate; M. Hafizh Arrafi; M. Nadhif Prasetyo; Annes C. Adma; M. Dias A. Monanda; Safyudin Safyudin; Medina Athiah
LOGISTA - Jurnal Ilmiah Pengabdian kepada Masyarakat Vol 5 No 2 (2021)
Publisher : Department of Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Andalas Kampus Limau Manis - Padang, Sumatera Barat Indonesia-25163

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/logista.5.2.31-36.2021

Pemberian ASI eksklusif pada bayi telah dianjurkan oleh semua pihak. ASI tidak hanya dapat memenuhi kebutuhan energi bayi, tetapi juga memberikan makronutrien dan mikronutrien yang penting untuk tumbuh kembang bayi. Walaupun demikian, cakupan pemberian ASI eksklusif belum dapat dikatakan optimal. Hal tersebut dipengaruhi oleh kurangnya pengetahuan ibu mengenai kandungan nutrisi ASI dan faktor ibu yang bekerja. Pengabdian masyarakat ini bertujuan untuk memberikan edukasi kepada ibu menyusui mengenai kandungan ASI dan pemberian ASI perah (ASIP). Kegiatan dilakukan secara online melalui Zoom. Kegiatan berupa seminar atau penyuluhan dengan menghadirkan 3 topik dan 3 narasumber. Evaluasi keberhasilan kegiatan dilakukan dengan menilai suasana diskusi dan memberikan pertanyaan tertutup melalui pra dan pascates. Sebanyak 29 ibu menyusui mengikuti kegiatan ini dengan rerata usia 29 tahun dan sebagian besar bekerja (80%). Para peserta antusias memberikan pertanyaan kepada para narasumber. Hasil evaluasi pra dan pascates menunjukkan ada peningkatan pengetahuan ibu menyusui sebesar 31% (p=0,000). Kegiatan pengabdian masyarakat berupa edukasi kandungan ASI dan pemberian ASIP berhasil dilakukan dengan baik. Edukasi mampu meningkatkan pengetahuan ibu menyusui. Edukasi atau penyuluhan dapat dilakukan untuk meningkatkan pengetahuan atau pemahaman masyarakat.Abstrak ditulis dalam bahasa Indonesia dan Inggris. Kata kunci: ASI, ASIP, edukasi, nutrisi, pengetahuan ABSTRACT Exclusive breastfeeding for babies has been recommended. It fulfills the baby's energy needs and provides nutrients that are necessary for baby's growth and development. However, the coverage of exclusive breastfeeding has not yet been sufficient, that is affected by the mother’s lack of knowledge about breast milk and the mother’s occupation. This community service aims to provide education to breastfeeding mothers about the content of breast milk and expressed breast milk to the breastfeeding mothers. The activities were performed by presenting 3 experts using online Zoom. Evaluation was done by assessing the atmosphere of the discussion and establishing closed questions through pre and post-test. A total of 29 breastfeeding mothers have participated in this activity with an average age of 29 years and most of them have job (80%). The participants were actively asking questions to the speakers. The results of the pre- and post-test evaluation showed that there was an increase in knowledge of breastfeeding mothers by 31% (p=0.000). It concludes that community service in the form of education activities on the content of breast milk and breastfeeding has been successful. Education or outreach activities has been proven to increase public’s knowledge and understanding of certain topic. Keywords: breast milk, education, expressed breast milk, knowledge, nutrition

Effect of glucose on reduced glutathione level in Malay uncomplicated type 2 diabetes patients Subandrate; Raafqi Ranasasmita
Acta Biochimica Indonesiana Vol. 4 No. 1 (2021): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.13

Background: Increasing blood sugar level may increase free radical compounds in type 2 diabetes. Free radical compounds can cause oxidative stress, thereby decreasing endogenous antioxidants such as reduced glutathione (GSH). Objective: This study aimed to determine whether random blood glucose levels affect GSH in type 2 diabetes patients within the Malay race. Methods: This study was observational with case-control, involving 25 patients with uncomplicated type 2 diabetes (receiving metformin and/or glimipiride) and 25 healthy controls. Random blood glucose levels were determined using ACCU-CHECK® Kit. Blood GSH levels were determined by Sigma GSH Assay Kit. Results: Results show that type 2 diabetes patients have a significantly lower random blood glucose level compared with those of age-matched normal subjects (p<0.0001). Type 2 diabetic patients had significantly lower levels of GSH (p=0.00) than those of age-matched normal subjects. We found a moderate negative correlation (r=-0.437 and p=0.02) between the level of random blood glucose and the level of GSH. Conclusion: The depletion of GSH during hyperglycemia may neutralize the free radicals indirectly generated by the abundant of glucose.

The Role of Vascular Endothelial Growth Factorin Thrombocytosis in Colorectal Cancer Patients: The Role of VEGF in Thrombocytosis Subandrate Subandrate; Dwi Indira Setyorini; Mediarty Mediarty; Irsan Saleh
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 2 No. 2 (2018): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v2i2.45

Backgorund: Colorectal cancer was included in a group of cancer with various complications. One complication that was often a cause of morbidity and mortality was thrombocytosis. In colorectal cancer, the incidence of thrombocytosis associated with the formation of blood vessels around the tumor or angiogenesis. Factors that played an important role in angiogenesis was vascular endothelial growth factor (VEGF). Methods: This study was an observational analytic research in colorectal cancer patients to determine the correlation levels of platelets and serum VEGF levels. A total of 33 patients with colorectal cancer at the Palembang Mohammad Hoesin Hospital be research subjects to examine the levels of platelets and levels of VEGF. The level of serum VEGF was performed using ELISA technique from SIGMAÂ®. Results: The average level of the patient's platelets was281,090.9Â±105,860.8/mm3. In this study, two patients (6.06%) have thrombocytosis.The average serum levels of VEGF research subjects were 221.2 Â± 152.8 pg/mL.Correlation test of levels of serum VEGF and platelets levels showed the value of p=0.040 (p> 0.05) and r = 0468. Conclusions: Thus, it can be concluded that in this research serum VEGF levels are almost always causes an increase in platelet levels in patients with colorectal cancer.

Inhibitory Effect of N-Hexane Fraction of Cherry Parasite (Dendrophthoe pentandra L.) on Alpha Glucosidase Sadakata Sinulingga; Putriana Fuji Safitri; Subandrate
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 6 No. 5 (2022): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v6i5.399

Background. Cherry parasite (Dendrophthoe pentandra L.) is a semi-parasitic plant that takes food from host body, so that phytochemical same as host plant. These phytochemicals have effect to inhibiting the action of the enzyme alpha glucosidase, such as flavonoids, saponins, tannins, alkaloids, and terpenoids/steroids. The aim of this study is to find out the effect of the cherry parasite to inhibiting the enzyme alpha glucosidase. Methods. This is an in vitro experimental study. Cherry parasite was fractionated with n-hexane and reacted by enzyme. Acarbose used as a positive control. The inhibitory effect on the alpha glucosidase was determined from the IC50 value by measuring the absorbance of p-nitrophenol using spectrophotometry as a result of the reaction of the enzyme. Screening phytochemical to determine the phytochemical of n-hexane fraction. This research was conducted at the Laboratory of Biochemistry and Medical Chemistry, Faculty of Medicine, Universitas Sriwijaya, Palembang. Results. The yield of n-hexane fraction was 7%. Phytochemical test showed that the sample contain flavonoids and terpenoids. IC50 value of n-hexane fraction was 106,333 ppm and categorized not active. Conclusion. The n-hexane fraction of cherry parasite contains flavonoids and terpenoids that considered not active to inhibiting the action of the enzyme alpha glucosidase.

EFEK ANTIHIPERGLIKEMIK EKSTRAK ETANOL DAUN BENALU KERSEN (Dendrophthoe pentandra (L.) Miq) PADA TIKUS PUTIH JANTAN YANG DIINDUKSI ALOKSAN Adiyatma Putra Mahardika; Sadakata Sinulingga; Medina Athiah; Subandrate Subandrate
Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Vol 6 No 2 (2021): JIIS
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

This study aims to determine the effect of lowering blood glucose. The study was conducted in an experimental laboratory in vivo with one group randomized prepost-test design. The extract was made through the gradual maceration process of Benalu Kersen leaf simplicia with ethanol solvent. The extract was divided into three groups with doses of 65 mg / kg, 130 mg / kg, and 260 mg / kg of body weight. The sample used was 9 male white rats (Rattus norvegicus) which were randomly divided into three groups. Blood glucose levels were measured before induction, after induction, after 7 days, and after 14 days of giving the extract. Data analysis was performed in the form of homogeneity test, normality test, paired t-test using Excel and SPSS programs. There was significant decrease in blood glucose levels on day 0 to day 7 in dose II group. The largest percentage reduction in blood glucose levels was shown in dose II group, namely 32.6%, followed by dose I group at 18.89%. The ethanol extract of Benalu kersen leaves did have an effect of lowering blood glucose levels on alloxan-induced male white rats.

Efek Antihiperglikemik Infusa Daun Benalu Kersen (Dendrophthoe pentandra (L.) Miq) Pada Tikus Putih Jantan Yang Diinduksi Aloksan Fatihah Az Zahra; Subandrate Subandrate; Safyudin Safyudin; Sadakata Sinulingga
Jurnal Fitofarmaka Indonesia Vol 9, No 1 (2022): JURNAL FITOFARMAKA INDONESIA
Publisher : Faculty of Pharmacy, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/jffi.v9i1.806

Kersen parasite leaves (Dendropthoe pentandra (L.) Miq) is a type of parasite that is hemiparasite and contains active compounds, namely, flavonoids, terpenoids and tannins. This compound has benefits as an antihyperglycemic. The aim of this study is to determine the effect of lowering blood glucose levels from the infusion of Kersen parasite leaves (Dendrophthoe pentandra (L.) Miq) in white male rats induced by alloxan. This research was a laboratory experimental study with a randomized pre- and posttest one group design. The study used alloxan-induced male rats which were divided into 3 dosage groups (38 mg/kgBW, 77 mg/kgBW, and 154 mg/kgBW). In the results of the mean blood glucose levels of rats, there was a decrease in blood glucose levels on the 0th day of treatment, on the 7th day after the treatment there was an increase in blood glucose levels and on the 14th day after the treatment there was a decrease in blood glucose levels except in the dose II group and III. In this study, Kersen parasite leaf infusion had no effect on reducing blood glucose levels of alloxan-induced male white rats.

Hemostatic Effect of Ethanol Extract of Piper betle, Linn Leaves to Male Mice Sadakata Sinulingga; Subandrate Subandrate; Bebbi Arisya Kesumaputri; Galuh Anggraini
Molekul Vol 12, No 1 (2017)
Publisher : Universitas Jenderal Soedirman

Hemorrhage occurs in most of the dental care. Untreated hemorrhage could cause excessive blood loss, hypotension, and cyanosis. A Natural resource that reported has an hemostatic effect is ethanol extract of betel leaves (Piper betel, Linn).The aim of this study is to find the minimum concentration of ethanol extract of betel leaves which capable of shortening the bleeding time in mice. The experimental study used pretest-posttest with control group design was conducted on 35 mice that divided into 7 group which are negative control, positive control (feracrylum 1%), the ethanol extract of betel leaves 1%, 5%, 10%, 15%, and 20%. All mice were injected heparin intravenously. Miceâs tail was cut at diameter 3 mm and pretest bleeding time was counted. Miceâs tail was recut at diameter 4 mm, given treatment for 5 seconds and posttest bleeding time was counted. Results of paired t-test showed that reduction of bleeding time between pretest and posttest was significant (p<0,050). The enhancement of ethanol extract of betel leaves concentration leads to better hemostatic effect. Results of ANOVA test showed that comparison of posttest bleeding time among groups was significant (p<0,050). The minimum concentration of ethanol extract of betel leaves which capable of shortening the bleeding time in mice is 5%.

Cytotoxicity, Antiproliferative and Apoptotic Effect of n-Hexane Fraction of Lime Parasite (Dendrophtoe pentandra) Subandrate Subandrate; Masayu Farah Diba; Salni Salni; Triwani Triwani; Sri Nita
Molekul Vol 14, No 1 (2019)
Publisher : Universitas Jenderal Soedirman

Breast cancer is one of the biggest causes of death in women in the world. Lime parasite (Dendrophtoe pentandra (L.) Miq.), a folk remedy used by Indonesian people, is believed to be efficacious as anticancer drug. This research aims to know the activity of n-hexane fractions of lime parasite in inhibiting the proliferation and apoptosis of T47D cells in vitro. Cytotoxic test with MTT method assay from n-hexane fractions used a multilevel concentration. Antiproliferative test was carried out by the method of MTT assay and cell doubling time was calculated at the time of duplication. Apoptotic test was done with concentration of 1 IC50and ½ IC50which was analyzed by flow cytometry. The results reveals that fractions of lime parasite have cytotoxic activity with concentration of IC50is included in moderatecytotoxic level. The result of the doubling time of the optimum fraction of n-hexane is in 31 hours with the concentration of ¼IC50. Results for the flow cytometry shows the fraction of n-hexane does not induce apoptosis in cells of T47D. Those results show that the active fraction of lime parasite has cytotoxic activity which is able to inhibit proliferation, but does not induce apoptosis of T47D cell.

Antioxidant Potential Of Lansium Domesticum Corr. Seed Extract In White Male Rat (Rattus Novergicus) Induced By Alcohol Subandrate Subandrate; Sadakata Sinulingga; Sri Wahyuni; M Fakhri Altiyan; Fatmawati Fatmawati
Molekul Vol 11, No 1 (2016)
Publisher : Universitas Jenderal Soedirman

Lansium domesticum Corr. is a typical plant of Indonesia. The seeds have a lot of useful compounds, such as flavonoids. Flavonoids are a natural compound that can act as antioxidants. The aim of this study was to determine the antioxidant potential of Lansiumdomesticum Corr. seed extract. This study was an experimental research with posttest control group design. The subjects were 32 white male rats. Rats were randomly divided into 4 groups: negative control group was given suspension of Na CMC 1%, dose group I, II and III were given Lansium domesticum Corr. seed extract with each dose of 100mg/kg, 200 mg/kg, and 300 mg/kg. Observation of the subject's response was done by measuring the levels of glutathione (GSH) and malondialdehyde (MDA). Analysis ANOVA test showed that there was a group that had an average difference both GSH levels (p=0.00) and MDA levels (p=0.00). Post hoc test analysis for GSH levels and MDA levels showed that only a dose of 100 mg/kg which have significant differences (p=0.00). Thus, Lansiumdomesticum Corr. seed extract has antioxidant potential and optimum dose of 100 mg/kg.

Efek minyak ikan toman (Channa micropeltes) terhadap kadar profil lipid mencit jantan (Mus musculus) model dislipidemia Sadakata Sinulingga; Aprilia Putri; Intan Rahma Dewi; Melina Indah Sari; Mutiara Budi Azhar; Tri Suciati; Mariana Mariana; Subandrate Subandrate
Majalah Kedokteran Andalas Vol 42, No 2 (2019): Published in May 2019
Publisher : Faculty of Medicine, Universitas Andalas

Tujuan: Untuk mengetahui efek minyak ikan toman (Channa micropeltes) terhadap profil lipid pada mencit jantan (Mus musculus). Metode: Penelitian ini adalah eksperimental laboratorium menggunakan rancangan randomized pre- dan post-test dengan menggunakan hewan coba yaitu 35 ekor mencit jantan (Mus musculus) yang telah diinduksi model hiperkolesterol menggunakan pakan tinggi lemak, kemudian dikelompokkan menjadi 7 kelompok untuk perlakuan, yaitu: kontrol negatif, kontrol positif dengan Simvastatin, kontrol positif dengan Gemfibrozil, minyak ikan dosis I (0,05g/30gBB), minyak ikan dosis II (0,1g/30gBB), minyak ikan dosis III (0,2g/30gBB), dan minyak ikan dosis IV (0,4g/30gBB). Pemeriksaan profil lipid dilakukan dengan mengambil sampel darah mencit dan diperiksa dengan menggunakan spektrofotometer. Hasil: Minyak ikan toman dapat menurunkan kadar kolesterol total, Low-Density Lipoprotein (LDL), dan trigliserida pada mencit dengan Dosis II, dosis III, dan dosis IV sedangkan untuk kadar High-Density Lipoprotein (HDL) dapat turun pada dosis iv. Simpulan: Minyak Ikan toman dapat menurunkan kadar profil lipid pada mencit jantan model dislipidemia.

Co-Authors Adiyatma Putra Mahardika Adma, Annes Claudia Ahmad, Zen Aldri Frinaldi Alexander Theo Yuda Salean Anggraini Tiara Septiyana Gunawan Aninditia, Fahira Aninditia, Fahira Annes C. Adma Aprilia Putri Arifin, Mutmainah Arrafi, Muhammad Hafizh Athiah, Medina Aulia Firdha Tariza Ayeshah A Rosdah Az Zahra, Fatihah Bebbi Arisya Kesumaputri Danang Bagus Untoro Dazakiah Dazakiah Dita Tri Ramadianti Dwi Indira Setyorini Dwi Indira Setyorini Dwirini Retno Gunarti Dzakiyah, Dzakiyah Dzakiyah, Dzakiyah Eka Febri Zulissetiana Eka Handayani Oktharina Ella Amalia Ella Amalia, Ella Ellla Amalia Erial Bahar Erial Bahar Eva Syahriana Eva Syahriana Evlin Kohar Ezra Hans Soputra Fadel Fikri Suharto Fadel Fikri Suharto Fahira Aninditia Fahira Aninditia Faisal, Mia EPA Fatihah Az Zahra Fatmawati Fatmawati Fatmawati Fatmawati Fatmawati Fatmawati Fatmawati Fatmawati Fatmawati Fatmawati, Fatmawati Fifa Argentina Firdaus, Mutia Muthmainnah Galuh Anggraini Hariyadi, Kusumo Hayati, Lusiya Hayati, Lusiya Hermansyah Hermansyah Hermansyah Hestiningsih, Tyas Hutabarat, Yosafat Beltsazar Iche Andriyani Liberty Iche Andriyani Liberty, Iche Andriyani Intan Rahma Dewi Irfannuddin Irfannuddin Irfannudin Irfannudin Irsan Saleh Izzah, Puspita Nurul Kohar, Evlin Kohar, Evlin Kusumo Hariyadi Kusumo Hariyadi Kusumo Hariyadi Kusumo Haryadi Liniyanti D. Oswari Liniyanti D. Oswari Lusia Hayati M Fakhri Altiyan M Fitra Romadhon M. Dias A. Monanda M. Hafizh Arrafi M. Hafizh Arrafi M. Nadhif Prasetyo M. Nadhif Prasetyo Mariana Mariana Mario Saputra Masayu Farah Diba Masayu Farah Diba Mediarty Mediarty Mediarty Mediarty Medina Athiah Medina Athiah Medina Athiah Medina Athiah Medina Athiah Melina Indah Sari Mgs Irsan Saleh Mgs. Irsan Saleh Mgs. Irsan Saleh Mia EPA Faisal Mia Esta Poetri Afdal Faisal, Mia Esta Poetri Afdal Mohamad Sadikin Mohamad Sadikin MOHAMAD SADIKIN Monanda, M Dias Athallah Monanda, M. Dias Athallah Muhammad Irsan Saleh Muniaty Sulam Ng Muthmainnah Arifin Mutiara Budi Azhar Naomi W Tioline Natasha, Amanda Ng, Muniaty Sulam Nita Parisa Nudhar, Lathifah Nur Azizah Nurul Windi Anggraini Nurul Windi Anggraini, Nurul Windi Oktalisa, Wenni Oktharina, Eka Handayani Oswari, Liniyanti Oswari, Liniyanti D Permono, Trijoso Putriana Fuji Safitri Raafqi Ranasasmita Rasyid, Riana Sari Puspita Ray Suga Aulia Ray Suga Aulia Sentani Riana Sari Puspita Rasyid Rini Yana Rini Yana Rini Yana Rizki Andini Nawawi Romadhon, M Fitra Sabathania, Hana Daini Sadakata Sinulingga Sadakata Sinulingga Sadakata Sinulingga Sadakata Sinulingga Safyudin Safyudin Safyudin Safyudin Safyudin Safyudin Safyudin Safyudin Safyudin Saleh, Mgs. M. Irsan Salni Salni Salni Salni Salni Salni Salni Santosa, Dorothy Eunike Setyorini, Dwi Indira Soputra, Ezra Hans Soputra, Ezra Hans Sri Hartini Harianja Sri Nita Sri Nita Sri Nita, Sri SRI RAHAYU Sri Wahyuni Suly Auline Rusminan Susilawati Susilawati Susilawati Susilawati Susilawati, Susilawati Tri Suciati Trijoso Permono Trijoso Permono Triwani Triwani Untoro, Danang Bagus Untoro, Danang Bagus Yana, Rini Yudianita Kesuma, Yudianita Zen Hafy

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