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Hematological Profile of Tilapia (Oreochromis niloticus) as a Bioin-dicator to Evaluate Water Quality of Floating Net Cage Cultivation System in Ranu Grati, Indonesia Alfarisi, Muhammad Asnin; Hertika, Asus Maizar Suryanto; Yanuhar, Uun
Journal of Aquaculture and Fish Health Vol. 14 No. 3 (2025): JAFH Vol. 14 No. 3 September 2025
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v14i3.75605

Abstract

Ranu Grati is a volcanic lake situated in Grati District, Pasuruan Regency, East Java, Indonesia. The rapid expansion of floating net cage aquaculture in Ranu Grati has raised concerns about water quality deterioration and its impact on fish health. This study aimed to evaluate the hematological profile of tilapia (Oreochromis niloticus) as a bioindicator for assessing water quality in floating net cage cultivation systems. The purpose of this research was to examine the hematological profile of tilapia fish alongside a series of water quality parameter measures as an environmental factor influencing fish life. Sampling was conducted with three repetitions every three weeks from September to November 2024 at five distinct locations, which were deemed representative of each group of floating net cages distributed around the Ranu Grati region. The Canonical Correspondence Analysis (CCA) approach was utilized to ascertain the relationship between hematological profile of tilapia and water quality. The data analysis indicates that the water quality at Stations 2 and 3 in the research area was somewhat polluted, but Stations 1 and 5 were heavily polluted. The elevated pollution levels at Stations 1 and 5 are attributed to the significant quantity of floating net cages in the vicinity relative to other stations. The investigation indicated that low dissolved oxygen levels and elevated ammonia concentrations impacted the hematological parameters of Tilapia fish. All water quality characteristics influenced the status of leukocytes, erythrocytes, and hemoglobin at moderate quantities. Moreover, micronuclei and hematocrit levels were significantly affected by elevated concentrations of ammonia, temperature, BOD, and pH, whereas DO, TDS, and TSS influenced lower values.
EFFECTS OF SPINACH (Amaranthus sp.) EXTRACT SUPPLEMENTATION ON MOLTING DURATION AND PHYSIOLOGICAL RESPONSES OF MUD CRABS (Scylla sp.) REARED IN CRAB APARTMENT SYSTEMS Sutrisno, Bagas Gagat Rahina Asihing; Supriatna, Supriatna; Uzair, Hafiz Muhammad; Ismail, Azwar; Hertika, Asus Maizar Suryanto
Jurnal Riset Akuakultur Vol 20, No 2 (2025): Juni (2025)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.20.2.2025.165-180

Abstract

This study investigates the effects of dietary supplementation of spinach (Amaranthus sp.) extract, rich in phytoecdysteroids, on the molting process and physiological parameters of mud crabs (Scylla sp.) cultivated in crab apartment boxes integrated with a recirculating aquaculture system. This experiment was designed using a completely randomized design (CRD) with four different doses of spinach extract as treatments (0, 300, 700, and 1000 ng g-1 of crab) and three replications. Some parameters measured were molting duration, hormone levels, including ecdysteroid and molt inhibiting hormone (MIH), reactive oxygen species (ROS), and water quality. The results showed that higher doses of spinach extract significantly shortened the molting duration (p < 0.05), with the fastest molting observed at 1000 ng g-1 (11.83 ± 1.26 days). Increased spinach extract doses also significantly affected MIH and ROS levels. Specifically, MIH levels increased from 12.05 ng mL-1 in the control to 20.88 ng mL-1 in treatment D, while ROS levels rose from 116.80 to 147.33 µmol mL-1. Overall, the administration of phytoecdysteroids from spinach presents a promising, eco-friendly approach to enhance soft-shell crab production efficiency, although careful dose optimization is necessary to balance production acceleration with animal welfare.Penelitian ini mengkaji pengaruh suplementasi pakan dengan ekstrak bayam (Amaranthus sp.) yang kaya akan fitoekdisteroid terhadap proses molting dan parameter fisiologis kepiting bakau (Scylla sp.) yang dibudidayakan dalam kotak apartemen kepiting terintegrasi dengan sistem akuakultur resirkulasi. Percobaan ini dirancang menggunakan rancangan acak lengkap (RAL) dengan empat dosis ekstrak bayam sebagai perlakuan (0, 300, 700, dan 1000 ng g⁻¹ kepiting) dan tiga ulangan. Beberapa parameter yang diukur meliputi durasi molting, kadar hormon termasuk ekdisteroid dan molt inhibiting hormone (MIH), reactive oxygen species (ROS), serta kualitas air. Hasil penelitian menunjukkan bahwa peningkatan dosis ekstrak bayam secara signifikan memperpendek durasi molting (p < 0,05), dengan waktu molting tercepat pada dosis 1,000 ng g⁻¹ (11,83 ± 1,26 hari). Peningkatan dosis ekstrak bayam juga berpengaruh nyata terhadap kadar MIH dan ROS. Secara khusus, kadar MIH meningkat dari 12,05 ng mL⁻¹ pada kontrol menjadi 20,88 ng mL⁻¹ pada perlakuan D, sedangkan kadar ROS naik dari 116,80 menjadi 147,33 µmol mL⁻¹. Secara keseluruhan, pemberian fitoekdisteroid dari bayam menunjukkan potensi sebagai pendekatan ramah lingkungan untuk meningkatkan efisiensi produksi kepiting cangkang lunak, meskipun demikian optimasi dosis tetap diperlukan agar tercapai keseimbangan antara percepatan produksi dan kesejahteraan hewan.
Pendampingan Pembuatan Pakan Alternatif Pasta Apung Berbasis Potensi Lokal di Pokdakan PAKWALI, Desa Bakalan, Kecamatan Purwosari, Kabupaten Pasuruan: Assistance in Making Alternative Floating Paste Feed Based on Local Potential in the PAKWALI Fish Farming Group, Bakalan Village, Purwosari District, Pasuruan Regency Hertika, Asus Maizar Suryanto; Nurlaelih, Euis Elih; Putra, Renanda Baghaz Dzulhamdhani Surya; Alfarisi, Muhammad Asnin; Aisyah, Jumrotul; Rahmadia, Alvira Rizqi; Prananda, Nauval Rifqi; Yudhistira, Ikmal Fahmi
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 9 No. 12 (2024): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v9i12.8282

Abstract

The aquaculture sector plays an important role in supporting food security and improving the community's economy. Aquaculture, especially in rural areas, has become one of the main pillars in providing protein sources for the community and also driving the local economy. This community service activity aims to improve the skills and independence of fish farmers in the Fish Farmers Group (PAKWALI) of Bakalan Village, Pasuruan Regency through the innovation of alternative floating paste feed based on local potential. The main problems faced by partners are the high cost of commercial feed and the lack of knowledge about making independent feed. The methods used include counseling, training, and assistance in making alternative feed. The main innovation is the development of floating paste feed that uses local raw materials such as tofu dregs and bran. The results of the activity showed an increase in the skills of partners in producing independent feed, with production cost efficiency reaching 40.3% compared to commercial feed. This alternative feed also increases the efficiency of nutrient absorption in fish and reduces feed waste. In conclusion, this program has succeeded in increasing the independence of fish farmers, reducing production costs, and encouraging more environmentally friendly and sustainable cultivation practices.
Mikromorfologi Gonad dan Tipe Reproduksi Ikan Lempuk (Gobiopterus sp.) sebagai Informasi untuk Upaya Domestikasi Latifa, Yusria; Farikhah, Farikhah; Hertika, Asus Maizar Suryanto
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.10840

Abstract

Lempuk fish is a native Indonesian fish that is endemic in the Ranu Grati tectonic lake, Pasuruan Regency, East Java. This research aims to provide information in efforts to domesticate lempuk fish, especially on reproductive aspects related to the microscopic characteristics of gonads and reproductive type of Lempuk fish (Gobiopterus sp). This research uses the primary sexual characteristics method with gonadal histology. The variables observed included the male to female sex ratio, the histological structure of male and female gonads, and the reproductive type of lempuk fish. All data was analyzed descriptively, qualitatively and quantitatively by comparing with related literature. The samples taken in this research were 39 Lempuk Fish (Gobiopterus sp). The samples were then preserved in 10% formalin. The number of samples for histological analysis was 20 individuals, while for morphometric and meristic analysis there were 8 individuals. The results of the research show that morphologically the lempuk fish has a transparent body shape and there is a central line on the body with black or brown pigment and has a total length of around 2-3 cm. Lempuk fish have a type of reproduction that develops asynchronously (asynchronous) because the development of oocytes in the ovaries of female fish shows a variety of developmental stages, namely the initial growth stage (nuclear and perinuclear chromatin), cortical alveolar, vitellogenesis, and the mature stage. The sex ratio is predominantly female with a ratio of 0.64: 0.36. The histological structure of male and female gonads has histological differences that reflect different reproductive functions, where the ovaries play a role in egg cell production, while the testes are responsible for sperm formation. An imbalance in the sex ratio indicates the importance of carrying out domestication steps in order to prevent extinction or continuous population decline.
HUBUNGAN KELIMPAHAN MIKROPLASTIK DENGAN KERUSAKAN HISTOPATOLOGIS PADA INSANG DAN USUS UDANG VANAME (Litopenaeus vannamei) YANG DIBUDIDAYAKAN DI TAMBAK DI PROBOLINGGO, JAWA TIMUR, INDONESIA Putra, Andhika Farras Rahardian; Hertika, Asus Maizar Suryanto; Maimunah, Yunita
Jurnal Riset Akuakultur Vol 20, No 1 (2025): Maret (2025)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.20.1.2025.63-77

Abstract

Peningkatan kontaminasi mikroplastik di lingkungan tambak menjadi ancaman serius bagi kesehatan udang vaname (Litopenaeus vannamei). Penelitian ini bertujuan untuk menganalisis hubungan antara kelimpahan mikroplastik pada insang dan usus dengan tingkat kerusakan histopatologis udang vaname yang dibudidayakan di tambak yang berlokasi di Probolinggo, Jawa Timur, Indonesia. Penelitian dilakukan secara deskriptif kuantitatif menggunakan analisis korelasi Spearman. Sampel udang diambil dari tiga lokasi tambak dan dianalisis secara histologis untuk menilai skor kerusakan jaringan. Hasil menunjukkan korelasi sangat kuat dan signifikan antara kelimpahan mikroplastik dengan skor kerusakan insang (r = 0,815; p-value = 0,007), degenerasi usus (r = 0,885; p-value = 0,002), nekrosis (r = 0,804; p-value = 0,009), dan inflamasi (r = 0,688; p-value = 0,041). Temuan ini menunjukkan bahwa mikroplastik berkontribusi besar terhadap kerusakan struktural organ respirasi dan pencernaan udang. Penelitian ini menegaskan pentingnya pengelolaan pencemaran mikroplastik dalam sistem budidaya berkelanjutan. The increasing presence of microplastic contamination in shrimp ponds poses a serious threat to the health of whiteleg shrimp (Litopenaeus vannamei). This study aimed to analyze the relationship between microplastic abundance in gills and intestines with the level of histopathological damage in whiteleg shrimp cultivated in ponds located in Probolinggo, East Java, Indonesia. A quantitative descriptive method was used with Spearman correlation analysis. Shrimp samples were collected from three pond locations and histologically examined to assess tissue damage scores. The results showed a very strong and significant correlation between microplastics abundance and gill damage (r = 0.815; p-value = 0.007), intestinal degeneration (r = 0.885; p-value = 0.002), necrosis (r = 0.804; p-value = 0.009), and inflammation (r = 0.688; p-value = 0.041). These findings indicated that microplastics significantly contribute to structural damage in the respiratory and digestive organs of shrimp. This study highlighted the importance of effectively managing microplastic pollution in sustainable aquaculture systems.
Assistance in Converting Tofu Waste Into Valuable Products for the Ngrangin Jaya Tofu and Tempeh Business Group in Sumberpasir Village, Pakis District, Malang Regency. Suryanto Hertika, Asus Maizar; Nurlaelih, Euis Elih; Amron, Kasyful; Supriyatin, Febriyani Eka
Journal of Innovation and Applied Technology Vol 11, No 2 (2025)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiat.2025.011.02.13

Abstract

This article describes the implementation of the Doctoral Program in comunity Service (Doktor mengabdi / DM) conducted in Sumberpasir Village, Pakis District, Malang Regency. The purpose of this service is to empower the community through the utilization of tofu industry waste into useful and eco-friendly products. The program consisted of three main activities: BIOLITA, which focused on processing liquid tofu waste into organic liquid fertilizer; MAGOLITA, which transformed solid tofu waste into maggot feed; and GURAME, which emphasized character building and educational development for elementary school students. The impact of this service involved improving environmental awareness, enhancing waste management practices, promoting community-based economic opportunities, and fostering cooperation between the university and the village. The program successfully demonstrated how higher education institutions can contribute to sustainable community development through applied science and innovation.
Analysis of Water Quality Correlation with the Immune Response of Litopenaeus vannamei in Probolinggo, East Java Andhika Farras Rahardian Putra; Asus Maizar Suryanto Hertika; Yunita Maimunah
International Journal of Marine Engineering Innovation and Research Vol. 10 No. 2 (2025)
Publisher : Department of Marine Engineering, Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j25481479.v10i2.6375

Abstract

Whiteleg shrimp (Litopenaeus vannamei) is a major aquaculture commodity in Indonesia, valued for its resilience, rapid growth, and economic potential. However, water quality remains a critical factor influencing shrimp health, particularly their immune response. This study aimed to examine the correlation between water quality parameters and the immune responses of L. vannamei cultivated in Probolinggo, East Java. A descriptive quantitative method was employed, using simple random sampling across three pond sites. Water parameters measured included temperature, pH, dissolved oxygen (DO), salinity, ammonia, nitrate, total suspended solids (TSS), and total dissolved solids (TDS). Immune response indicators such as Total Hemocyte Count (THC), differential hemocyte count (DHC), and phagocytic activity were analyzed. Canonical Correspondence Analysis (CCA) was used to assess correlations. The results revealed significant relationships between water quality and shrimp immune parameters, highlighting the importance of proper environmental management in shrimp farming. These findings provide insights for improving productivity and disease resistance in sustainable aquaculture systems.
Immune Activity of Vannamei Shrimp (Litopenaeus vannamei) Under Ozonated and Non-Ozonated Treatments Moh. Wildanul Jannah; Asus Maizar Suryanto Hertika; Yenny Risjani; Wahidah Inayati
Jurnal Penelitian Pendidikan IPA Vol 11 No 2 (2025): February
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i2.9851

Abstract

This study aims to analyze the effect of ozonation treatment on the immune activity of vannamei shrimp, compared to non-ozonization treatment. Parameters measured include Total Haemocyte Count (THC), Differential Haemocyte Count (DHC), and phagocytic activity. The results show that ozonation treatment produces higher THC values, namely an average of 75% more than non-ozonation. The DHC value in the ozonation treatment showed an increase in granular cells by 40% compared to the non- ozonation treatment. In contrast, hyaline cells decreased due to a more active immune response against pathogens. Phagocytic activity in the ozonation treatment reached 15%, higher than non-ozonization which only reached 10.33%. These data indicate that ozonation plays an important role in improving shrimp's immune response by reducing the water's pathogen content and minimizing stress. In conclusion, ozonation was proven effective in increasing the immunity of vannamei shrimp by supporting key immunological parameters.
Comparison of Water Conditions and Growth Performance of Vannamei Shrimp (Litopenaeus vannamei) under Different Pond Management Systems (Superintensive, Intensive, and Traditional Culture Systems) Sri Astutik; Sri Andayani; Asus Maizar Suryanto Hertika
Jurnal Penelitian Pendidikan IPA Vol 11 No 6 (2025): June
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i6.10130

Abstract

Vannamei shrimp (Litopenaeus vannamei) is one of the high-value aquaculture commodities in the global market, known for its excellent adaptability to various environmental conditions, rapid growth, and high survival rates. This study aims to compare water conditions and the growth performance of vannamei shrimp under three different pond management systems: supra-intensive, intensive, and traditional. The analysis was conducted by measuring water quality parameters such as temperature, salinity, pH, dissolved oxygen, ammonia, and nitrate concentrations. Growth performance was evaluated through observations of Average Daily Gain (ADG) and Mean Body Weight (MBW). At 50 days of cultivation, the supraintensive system produced an average body weight of 11.85 grams with a daily growth rate of 0.2219 grams/shrimp/day. The intensive system resulted in slightly lower growth, with an average body weight of 8.6 grams and a daily growth rate of 0.1547 grams/shrimp/day. The traditional system showed the lowest growth performance, with an average weight of 6.66 grams and an ADG of 0.0265 grams/shrimp/day. The different pond management systems influence the dynamic risks of water quality changes based on input loads from feed waste and feces. The traditional system exhibits a slower rate of water quality changes compared to the intensive and supraintensive systems.
Sustainability Status of Vaname Shrimp (Penaeus vannamei) Pond in Coastal Areas of Lamongan Regency Destrina Elita Koto; Asus Maizar Suryanto Hertika; Jati Batoro
Jurnal Penelitian Pendidikan IPA Vol 11 No 12 (2025): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i12.13419

Abstract

Lamongan Regency was the largest center of the fisheries in East Java.  Lamongan Regency only has two type of operation ponds: traditional brackishwater pond and semi-intensive brackishwater pond. Aquaculture in Lamongan District still relies on traditional fisheries. Whiteleg shrimp (Penaeus vannamei) as a Indonesia`s leading aquaculture commodities can have a positive impact on coastal communities, especially in economic and social aspects but does not have sustainable waste management. In this study, a multidimensional scalling (MDS) method was used to analyze the ecological, economi, social, technology and institutional dimension. The study was conducted during February – May 2025, primary data collection was carried out throught interview respondens and the secondary data from related data references. The result of the study showed that the sustainability values range both 48,57 – 55,82 (ecological dimensions), 60,83 – 67,60 (economic dimensions), 55,48-66,70 (social dimensions), 54,79 – 57,57 (technological dimensions), and 50,83 – 57,90 (institutional dimensions). The averages value of sustainability index of vaname shrimp (Penaeus vannamei) Pond in Coastal Areas of Lamongan Regency was quite sustainability. Based on leverage analysis, sensitive attributes obtained in this study were ecological dimensions (1) influence are the level of pollution, (2) pest attacks and (3) water quality management, economic dimension (1) availability of capital resources, (2) marketing prices (RMS = 4.0), and (3) impact on income , social dimension (1) interaction patterns, (2) training and socialization and (3) cultivation knowledge technological dimensions (1) wastewater treatment plant (WWTP) technology, (2) machine automation, and (3) the availability of facilities and infrastructure, and institutional dimensions (1) monitoring and evaluation
Co-Authors ., Supriatna Agung Dwi Handoko Agustiansi Yeyen Qurniawatri Aisyah, Jumrotul Akhsan Fikrillah Paricahya Al Farida, Soraya Alifa Ni Mah Alby Ghifari Azhar Alfarisi, Muhammad Asnin Amin Setyo Leksono Amira Masitha Andhika Farras Rahardian Putra Andi Kurniawan Anjasmara, Aang Setyawan Aprillia Mifthakun Ni’mah Mifthakun Ni’mah Ni’mah Arief Andy Soebroto Arief Darmawan Arief Darmawan Arief Farhan Kurniawan Anasiru Awalia Oktaviana Ayu Winna Ramadhani Baghaz Dzulhamdhani Surya Putra, Renanda Baghaz, Renanda DSP Bagyo Yanuwiadi Bagyo Yanuwiadi Bimo Aji Nugroho Bimo Aji Nugroho Catur Budi Noviya Destrina Elita Koto Devi Rahmania Nureka Wasti Dewi Nur Setyorini Diana Arfiati Dini Anggraini Dini Sofarini Dyah Ayu Wijayanti Eka Supriatin, Febriyani Endang Yuli Herawati Ertikasari, Nuning Eryanto, Erik Evellin Dewi Lusiana Evellin Dewi Lusiana, Evellin Dewi Faizal Kasim Farikhah Farikhah, Farikhah Fisma Josara Apriliyanti Hafish Gigih Purnama Halimah, Marsa Fatin Handoko, Agung Dwi Happy Nursyam Herawati Umi S. Heru Suryanto Ilham Afandy Afandy Irawati Mei Widiastuti Islamy, R Adharyan Ismail, Azwar Ismail, Mahrus Iswati Iswati Ita Fitria S Jatayu, Diklawati Jati Batoro Jibril Maulana Kasyful Amron Kurnianingsih, Asmiyati Lal, Mohammad Tamrin Bin Mohamad Latifa, Yusria Listiya Gita Lesmana Made Dwipa Kusuma Maharani Maftuch Maftuch Masitha, Amira Miftahul Khair Kadim Miftahul Khair Kadim, Miftahul Khair Moh. Wildanul Jannah MOHAMAD FADJAR Mohammad Mahmudi Muhammad Arifin Gymnastyar Muhammad Dailami, Muhammad Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Sumsanto Mukhlis, Dharu Habib Neni Diah Kusumaning Arum Nico Rahman Caesar Ningsih, Hartati Kartika Nugroho, Bimo Aji Nurul Mutmainnah, Nurul Patresna, Laelatul Nisa Prananda, Nauval Rifqi Prasetyawati, Ranita Ayu Punto Apri Sembodo Putra, Andhika Farras Rahardian Putra, Renanda Baghaz D.S. Putra, Renanda Baghaz Dzulhamdani Surya Putra, Renanda Baghaz Dzulhamdhani Surya Qurniawatri, Agustiansi Yeyen Rahardjo, Seto Sugianto Prabowo Rahmadia, Alvira Rizqi Rahmi Nurdiani Ranita Ayu Prasetyawati Renanda Baghaz Dzulhamdhani Surya Putra Renanda Baghaz Dzulhamdhani Surya Putra Rizka Rahmana Putri Savira, Riza setya widi ayuning permanasari Shofiyatul Lailiyah Shofwan, Shofwan Sisca Fajriani Sri Andayani Sri Astutik Sujono Sujono Supriatin, Febriyani Eka Supriatna . Supriatna Supriatna Supriyatin, Febriyani Eka Sutrisno, Bagas Gagat Rahina Asihing TIWI NURJANNATI UTAMI Utari, Inpita Casuarina Eqisetia Uzair, Hafiz Muhammad Wahidah Inayati Wijanata, Ida Bagus Pascad Wijayanti, Dyah Ayu Yanu Iswahfiudin Yanuhar, Uun Yenny Risjani Yudhistira, Ikmal Fahmi Yuli, Endang Herawati Yuni Kilawati Yuniari, Shinta Hiflina Yunita Maimunah Zahrotur Rosyidah