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Pengamatan Jaringan Lambung Kijing Taiwan (Anodonta woodiana Lea) Yang Terdedah Pestisida Diazinon 60 EC Pada Beberapa Konsentrasi Lesmana, Listiya Gita; Arfiati, Diana; Maizar, Asus
The Journal of Experimental Life Science Vol. 3 No. 1 (2013)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1125.398 KB) | DOI: 10.21776/ub.jels.2013.003.01.07

Abstract

Jaringan lambung yang diamati berasal dari kijing (Anodonta woodiana) yang terdedah pestisida Diazinon 60 EC selama 2 minggu dengan pemberian dosis yang berbeda, yaitu 3,5x10-6 ; 1,75x10-6 ; 0,875x10-6 dan 0 ppm. Kondisi kijing diamati sebelum pemberian pestisida dan setelah 1 minggu dan 2 minggu pendedahan. Irisan lambung dibuat dari sampel 1 dan 2 minggu pendedahan menggunakan pewarnaan HE. Analisis histologi lambung kijing menunjukkan bahwa dinding lambung setelah terdedah pestisida akan mengalami kerusakan berupa penebalan dinding (edema), dengan jarak digestive divericula saling berjauhan, silia dalam lambung terlihat menyatu (fusi) dan terjadi pembengkakan silia (hyperplasia). Bahkan setelah pendedahan pestisida selama 2 minggu, dinding lambung makin menebal serta digestive diverticula mengalami pengerutan (atropi) dan pemecahan atau kerusakan sel (nekrosis).Kata Kunci : Anodonta woodiana, Diazinon 60 EC, Kerusakan lambung
An Analysis on Hemocytes Profile in Kijing Taiwan (Anodonta woodiana) and Water Quality in Freshwater Aquaculture Ponds Wijayanti, Dyah Ayu; Hertika, Asus Maizar Suryanto; Yanuwiadi, Bagyo
The Journal of Experimental Life Science Vol. 8 No. 1 (2018)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1318.717 KB) | DOI: 10.21776/ub.jels.2018.008.01.02

Abstract

Kijing Taiwan (Anodonta woodiana) is found in water with muddy substrate. Kijing Taiwan lives in bottom waters and is relatively settled. Kijing Taiwan is a filter feeder organism that can filter water and is able to survive in polluted environment, so it can be used as a bio-indicator of a water body. The study was conducted in the Public Hatchery Unit (UPR) Sumber Mina Lestari. Profile hemocytes in Taiwan Kijing influenced by environment pollution inducer among others. Hemocytes were observed consisting of Total Haemocyte Count (THC) and Differential Haemocyte Count (DHC). The results show that the profile of hemocytes (THC and DHC) of Kijing Taiwan is not polluted. Results from aquaculture pond water quality measurements indicate that the indicators of the water quality i.e. temperature, pH, dissolved oxygen, and ammonia are still within normal limits. However, in TOM test, BOD and soil organic matter has exceeded the normal limits. The existence of organic matter became the medium of life for Kijing Taiwan, due to its role as a biofilter among other organic materials. Keywords: Hemocytes Profile, Kijing Taiwan, Water Quality.
Lethal Concentration 50 (LC50 - 96 hours) Nile Tilapia (Oreochromis niloticus) exposed Cypermethrin-based Pesticide Yuniari, Shinta Hiflina; Hertika, Asus Maizar Suryanto; Leksono, Amin Setyo
The Journal of Experimental Life Science Vol. 6 No. 2 (2016)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (917.984 KB) | DOI: 10.21776/ub.jels.2016.006.02.01

Abstract

In aquaculture of an irrigation, the use of cypermethrin-based pesticide can harm nile tilapia which cultured within and its impact on humans who consume the fish. Thus it is necessary to study to determine the threshold of this cypermethrin-based pesticide. Determining the thresholds was using the test of LC50 - 96 hours (Lethal Concentration 50-96 hour) which tested tilapia that dead by 50%, which can be used as a benchmark threshold for cypermethrin-based pesticide. Test of LC50 - 96 hours consist of preliminary test and advanced test. Lethal Concentration 50 (LC50 - 96 hours) of cypermethrin-based pesticide towards nile tilapia (Oreochromis niloticus) is 0.082 ppm. At these doses, tilapia death reached 50%. This concentration is expressed as a threshold usage of cypermethrin-based pesticide on the environment around the aquaculture of tilapia. Keywords: Cypermehrin-based pesticide, LC5096 hour, Nile tilapia.
Assessing the Genotoxic Potentials of Methomyl-based Pesticide in Tilapia (Oreochromis niloticus) Using Micronucleus Assay Islamy, R Adharyan; Yanuhar, Uun; Hertika, Asus Maizar Suryanto
The Journal of Experimental Life Science Vol. 7 No. 2 (2017)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1172.773 KB) | DOI: 10.21776/ub.jels.2017.007.02.05

Abstract

Pesticides are recognized as serious pollutants in the aquatic environment with the potential to cause genotoxic on the aquatic organism, especially fish. The micronucleus (MN) assay has been used to evaluate genotoxicity of many compounds in polluted ecosystems such pesticides. The aim of this study to determine genotoxic effect of methomyl-based pesticide on tilapia (Oreochromis niloticus). Fish were exposed to six different concentrations base on range finding test (0 ppm, 3.2 ppm, 4.2 ppm, 6.5 ppm, 8.7 ppm and 10 ppm) of methomyl-based pesticide. The micronucleus were collected from peripheral blood erythrocyte of fish after 96 h exposure. Peripheral blood samples smears were stained with Giemsa, MN frequencies were counted and statistically analyzed using one-way ANOVA. The result of this study showed after 96 hours exposed to methomyl-based pesticide, at concentration 0 ppm causes 0% mortality, at concentration to 3.2 ppm causes 30% mortality, at concentration 4.2 ppm causes 60% mortality, at concentration 6.5 ppm causes 70% mortality, at concentration 8.7 ppm causes 80% mortality, at concentration 10 ppm causes 100% mortality of fish test. Lethal Concentration 50 (LC50 - 96 hours) of methomy-base pesticide towards tilapia (O. niloticus) is 4.015 ppm. Through micronuclei assay during 96 hour exposure of methomyl-based pesticide, the result shows that frequencies of micronuclei in erythrocyte of fish test at concentration at 0ppm is 12‰, 18‰ and 16‰; at concentration at 3.2ppm is 33‰, 26‰ and 29‰; at concentration at 4.2ppm is 41‰, 38‰ and 46‰; at concentration at 6.5ppm is 68‰, 81‰ and 82‰; at concentration 8.7ppm is 133‰, 130‰ and 137‰; at concentration 10ppm is 163‰, 166‰ and 156‰. It revealed that methomyl-based pesticide exposure induced after 96 h significantly (P<0.05) increased genotoxic potentials simultaneous with increased concentration.Keywords: Genotoxic, Methomyl, Micronucleus Assay, Pesticide, Tilapia.
Growth Rate and Chemical Composition of Secondary Metabolite Extracellular Polysaccharide (EPS) in Microalga Porphyridium cruentum Mutmainnah, Nurul; Risjani, Yenny; Hertika, Asus Maizar Suryanto
The Journal of Experimental Life Science Vol. 8 No. 2 (2018)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1296.901 KB) | DOI: 10.21776/ub.jels.2018.008.02.05

Abstract

Porphyridium cruentum microalgae have great potential to be developed as a source of active ingredients in various fields of health. It excretes secondary metabolites in the form of extracellular polysaccharides (EPS), potentially as antibacterial, antihyperglycemic, and immunostimulant components. This study aims to obtain the best culture density of P. cruentum, the active component of P. cruentum (EPS), and the chemical composition of EPS. The P. cruentum was cultivated on 15%, 20% and 25% culture stock, with an addition of silicate, Fe and vitamins in sterile seawater medium with salinity 35 ‰, pH 8, temperature 25-27°C, with continuous aeration and 2500 lux continuous 24 hours, for 14 days of culture period. The results of this study indicate differences in growth rates and abundance of microalgae to each culture stock. The 15%, 20% and 25% culture stocks each showed stationary phase at days 10th, 12th and 14th, with EPS concentrations of 10 ppt, 12 ppt, and 15 ppt, respectively. Variation of P. cruentum culture showed different EPS results, with a density of 25% capable of producing the highest EPS extract of 15,000 mg.L-1. The EPS is known to contain glucose and carboxylic acid compounds that can be utilized in the health and industrial fields. Keywords: culture, extracellular polysaccharides, Porphyridium cruentum.
The Alteration Tissue Structure of Digestive diverticulum of Anodonta woodiana Induced by Lead Putra, Renanda Baghaz Dzulhamdhani Surya; Andayani, Sri; Ningsih, Hartati Kartika; Arfiati, Diana; Hertika, Asus Maizar Suryanto
The Journal of Experimental Life Science Vol. 8 No. 2 (2018)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1617.255 KB) | DOI: 10.21776/ub.jels.2018.008.02.03

Abstract

Accumulated the toxic compound such as lead in A. woodiana caused an alteration in tissue. The aim of the present study is observation through histology to determine the alteration of the structure of digestive diverticulum tissue. A. woodiana were maintained in water tub that treated lead II nitrate exposure (Pb(NO3)2) 0, 15, 25, 35 mgL-1 for 7 days to determine the alteration of the structure of damaged the digestive diverticulum and determining water quality such temperature, DO and pH. The highest damage occurs in digestive diverticulum treated 35 mgL-1  due to the many alteration tissue structure such as hyperplasia, edema, atrophy, necrosis with the percentage of total damage of about 35%. the lowest alteration tissue structure occurs in 15 mgL-1 treatment group of the digestive diverticulum. the temperature was observed at about 25.3 - 26.4°C, pH about 6-7, and DO about 6.1-6.7 mgL-1. Thus, the increment of lead dose treatment induces increment of tissue structure alteration in digestive diverticula tissue, therefore, might promote the death of Anodonta woodiana. Keywords: Anodonta woodiana, digestive diverticulum, histology
Distribution of heavy metal (Pb, Cd and Hg) concentrations in sediment of Bone River, Gorontalo Kadim, Miftahul Khair; Yuli, Endang Herawati; Arfiati, Diana; Hertika, Asus Maizar Suryanto; Kasim, Faizal
Depik Vol 11, No 3 (2022): DECEMBER 2022
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.11.3.27775

Abstract

The concentration of three heavy metals Pb, Cd and Hg ware measured in Bone River, Gorontalo sediment. The heavy metal pollution on sediment in Bone River have not been explored longitudinally. The samples were taken in July 2021 at 8 stations. The trend of metals ware observed in sediment as PbCdHg. The level of studied metals Cd and Hg did not exceed the safe limit by ANZECC, OSQG LEL and CCME TEL meanwhile Pb was met the limit by by ANZECC. However, the investigated showed that fluctuations due to differences in the characteristics of each location. Contamination factor (CF) and geoaccumulation index (Igeo) demonstrated that the sediment samples were low contamination and lightly polluted. Contamination by heavy metals in the Bone River implies that the conditions are much frightening for the biota and residents around the river.
Hematological Profile of Bader Fish (Barbonymus altus) in the Brantas River, Blitar Region in Measurement of River Water Quality Alby Ghifari Azhar; Asus Maizar Suryanto Hertika; Andi Kurniawan
Jurnal Penelitian Pendidikan IPA Vol 10 No 5 (2024): May
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i5.4832

Abstract

Brantas River is habitat to a fish species known as the bader fish (Barbonymus altus). Research aims to evaluate the hematological profile of the bader fish as a means to assess the quality of the Brantas River in the Blitar region. Determining the waste flow from community activities that influences blood quality of bader fish is another goal of this research. To achieve this, various water quality indices were measured in the river and hematological parameters measured in the fish. Data collection was conducted once every week for three weeks during 17 January to 17 March 2022. The measurements aimed to observe any changes in the fish's hematological profile over this period. Two data analysis techniques were employed for the research: Pollution Index (IP) and Canonical Correlation Analysis (CCA). IP measurements reveal that all three stations share a common assessment, classifying them as having light pollution. When analyzing correlation measurements through CCA, it was found that erythrocytes exhibited a robust positive correlation with a pH value of 0.549, while conversely, DO (Dissolved Oxygen) showed a strong negative correlation with leukocytes with value -0.717. In summary, the water conditions in the Brantas region remain conducive for the habitat of bader fish.
Hematological Analysis of Red Bader Fish (Barbonymus altus) as a Biomarker in Assessing Pollution Status in Brantas River, Surabaya, East Java Arief Farhan Kurniawan Anasiru; Asus Maizar Suryanto Hertika; Uun Yanuhar
Jurnal Penelitian Pendidikan IPA Vol 10 No 2 (2024): February
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i2.5659

Abstract

The Brantas River has many functions for both humans and the aquatic organisms in it. The type of fish that is often found in the Brantas River in Surabaya is the red bader fish (Barbonymus altus). Hematology is a sensitive parameter and can be used as a marker in assessing water conditions. The aim of this research is to analyze the hematological profile of red bader fish (Barbonymus altus) to assess the condition of the Brantas River, Surabaya. The research was carried out at 3 locations on the Brantas River in Surabaya with 3 sampling times with an interval of 1 month starting in January – March 2022. The parameters observed were water quality (temperature, TSS, TDS, pH, DO, BOD, ammonia and heavy metals Hg) and hematological parameters (erythrocytes, hemoglobin, hematocrit, leukocytes and micronuclei). Analysis of the relationship between water quality parameters and hematology using the Correspondence Correlation Analysis (CCA) method. The results show that there are water quality parameters that do not meet quality standards and there are abnormal hematological parameters. CCA analysis shows that there is a correlation between water quality and hematological parameters with moderate concentrations (erythrocytes, hemoglobin and hematocrit) and strong concentrations (leukocytes and micronuclei).
Growth Analysis of Caulerpa lentillifera Cultivated at Laboratory Scale with Different Light Intensities Fisma Josara Apriliyanti; Yenny Risjani; Asus Maizar Suryanto Hertika; Akhsan Fikrillah Paricahya
Jurnal Penelitian Pendidikan IPA Vol 10 No 5 (2024): May
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i5.6126

Abstract

In Indonesia, Caulerpa sp. is often utilized as an edible food ingredient by eating it raw as a vegetable salad or as a vegetable. Different light intensities greatly affect the growth of sea grapes (Caulerpa lentillifera). However, this type of seaweed is still few who can cultivate it. Therefore, the purpose of the study was to analyze the growth performance of Caulerpa lentillifera seedlings at the cultivation stage in the laboratory with different light intensities. The research was conducted using the RAL method with 5 treatments and 4 replications. Light intensity (1,000, 2,000, 3,000, 4,000, 5,000 Lux) was applied during cultivation. The specific growth rate ranged from 0.0065%/day - 0.0061%/day. The number of ramuli of Caulerpa lentillifera ranged from 651 grains - 723 grains. The number of tallus Caulerpa lentillifera during 45 days of maintenance with various treatments ranged from 28 branches - 32 branches. The conclusion of this research is that light intensity significantly affects the growth of Caulerpa lentillifera.
Co-Authors ., Supriatna Agung Dwi Handoko Agustiansi Yeyen Qurniawatri Aisyah, Jumrotul Akhsan Fikrillah Paricahya Al Farida, Soraya Alifa Ni Mah Alby Ghifari Azhar Alfarisi, Muhammad Asnin Amin Setyo Leksono Amira Masitha Andhika Farras Rahardian Putra Andi Kurniawan Anjasmara, Aang Setyawan Aprillia Mifthakun Ni’mah Mifthakun Ni’mah Ni’mah Arief Andy Soebroto Arief Darmawan Arief Darmawan Arief Farhan Kurniawan Anasiru Awalia Oktaviana Ayu Winna Ramadhani Baghaz Dzulhamdhani Surya Putra, Renanda Baghaz, Renanda DSP Bagyo Yanuwiadi Bagyo Yanuwiadi Bimo Aji Nugroho Bimo Aji Nugroho Catur Budi Noviya Destrina Elita Koto Devi Rahmania Nureka Wasti Dewi Nur Setyorini Diana Arfiati Dini Anggraini Dini Sofarini Dyah Ayu Wijayanti Eka Supriatin, Febriyani Endang Yuli Herawati Ertikasari, Nuning Eryanto, Erik Evellin Dewi Lusiana Evellin Dewi Lusiana, Evellin Dewi Faizal Kasim Farikhah Farikhah, Farikhah Fisma Josara Apriliyanti Hafish Gigih Purnama Halimah, Marsa Fatin Handoko, Agung Dwi Happy Nursyam Herawati Umi S. Heru Suryanto Ilham Afandy Afandy Irawati Mei Widiastuti Islamy, R Adharyan Ismail, Azwar Ismail, Mahrus Iswati Iswati Ita Fitria S Jatayu, Diklawati Jati Batoro Jibril Maulana Kasyful Amron Kurnianingsih, Asmiyati Lal, Mohammad Tamrin Bin Mohamad Latifa, Yusria Listiya Gita Lesmana Made Dwipa Kusuma Maharani Maftuch Maftuch Masitha, Amira Miftahul Khair Kadim Miftahul Khair Kadim, Miftahul Khair Moh. Wildanul Jannah MOHAMAD FADJAR Mohammad Mahmudi Muhammad Arifin Gymnastyar Muhammad Dailami, Muhammad Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Sumsanto Mukhlis, Dharu Habib Neni Diah Kusumaning Arum Nico Rahman Caesar Ningsih, Hartati Kartika Nugroho, Bimo Aji Nurul Mutmainnah, Nurul Patresna, Laelatul Nisa Prananda, Nauval Rifqi Prasetyawati, Ranita Ayu Punto Apri Sembodo Putra, Andhika Farras Rahardian Putra, Renanda Baghaz D.S. Putra, Renanda Baghaz Dzulhamdani Surya Putra, Renanda Baghaz Dzulhamdhani Surya Qurniawatri, Agustiansi Yeyen Rahardjo, Seto Sugianto Prabowo Rahmadia, Alvira Rizqi Rahmi Nurdiani Ranita Ayu Prasetyawati Renanda Baghaz Dzulhamdhani Surya Putra Renanda Baghaz Dzulhamdhani Surya Putra Rizka Rahmana Putri Savira, Riza setya widi ayuning permanasari Shofiyatul Lailiyah Shofwan, Shofwan Sisca Fajriani Sri Andayani Sri Astutik Sujono Sujono Supriatin, Febriyani Eka Supriatna . Supriatna Supriatna Supriyatin, Febriyani Eka Sutrisno, Bagas Gagat Rahina Asihing TIWI NURJANNATI UTAMI Utari, Inpita Casuarina Eqisetia Uzair, Hafiz Muhammad Wahidah Inayati Wijanata, Ida Bagus Pascad Wijayanti, Dyah Ayu Yanu Iswahfiudin Yanuhar, Uun Yenny Risjani Yudhistira, Ikmal Fahmi Yuli, Endang Herawati Yuni Kilawati Yuniari, Shinta Hiflina Yunita Maimunah Zahrotur Rosyidah