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Growth Rate and Chemical Composition of Secondary Metabolite Extracellular Polysaccharide (EPS) in Microalga Porphyridium cruentum Mutmainnah, Nurul; Risjani, Yenny; Hertika, Asus Maizar Suryanto
The Journal of Experimental Life Science Vol. 8 No. 2 (2018)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1296.901 KB) | DOI: 10.21776/ub.jels.2018.008.02.05

Abstract

Porphyridium cruentum microalgae have great potential to be developed as a source of active ingredients in various fields of health. It excretes secondary metabolites in the form of extracellular polysaccharides (EPS), potentially as antibacterial, antihyperglycemic, and immunostimulant components. This study aims to obtain the best culture density of P. cruentum, the active component of P. cruentum (EPS), and the chemical composition of EPS. The P. cruentum was cultivated on 15%, 20% and 25% culture stock, with an addition of silicate, Fe and vitamins in sterile seawater medium with salinity 35 ‰, pH 8, temperature 25-27°C, with continuous aeration and 2500 lux continuous 24 hours, for 14 days of culture period. The results of this study indicate differences in growth rates and abundance of microalgae to each culture stock. The 15%, 20% and 25% culture stocks each showed stationary phase at days 10th, 12th and 14th, with EPS concentrations of 10 ppt, 12 ppt, and 15 ppt, respectively. Variation of P. cruentum culture showed different EPS results, with a density of 25% capable of producing the highest EPS extract of 15,000 mg.L-1. The EPS is known to contain glucose and carboxylic acid compounds that can be utilized in the health and industrial fields. Keywords: culture, extracellular polysaccharides, Porphyridium cruentum.
The Alteration Tissue Structure of Digestive diverticulum of Anodonta woodiana Induced by Lead Putra, Renanda Baghaz Dzulhamdhani Surya; Andayani, Sri; Ningsih, Hartati Kartika; Arfiati, Diana; Hertika, Asus Maizar Suryanto
The Journal of Experimental Life Science Vol. 8 No. 2 (2018)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1617.255 KB) | DOI: 10.21776/ub.jels.2018.008.02.03

Abstract

Accumulated the toxic compound such as lead in A. woodiana caused an alteration in tissue. The aim of the present study is observation through histology to determine the alteration of the structure of digestive diverticulum tissue. A. woodiana were maintained in water tub that treated lead II nitrate exposure (Pb(NO3)2) 0, 15, 25, 35 mgL-1 for 7 days to determine the alteration of the structure of damaged the digestive diverticulum and determining water quality such temperature, DO and pH. The highest damage occurs in digestive diverticulum treated 35 mgL-1  due to the many alteration tissue structure such as hyperplasia, edema, atrophy, necrosis with the percentage of total damage of about 35%. the lowest alteration tissue structure occurs in 15 mgL-1 treatment group of the digestive diverticulum. the temperature was observed at about 25.3 - 26.4°C, pH about 6-7, and DO about 6.1-6.7 mgL-1. Thus, the increment of lead dose treatment induces increment of tissue structure alteration in digestive diverticula tissue, therefore, might promote the death of Anodonta woodiana. Keywords: Anodonta woodiana, digestive diverticulum, histology
Co-Authors ., Supriatna Agung Dwi Handoko Agustiansi Yeyen Qurniawatri Aisyah, Jumrotul Al Farida, Soraya Alifa Ni Mah Alfarisi, Muhammad Asnin Amin Setyo Leksono Amira Masitha Anasiru, Arief Farhan Kurniawan Andhika Farras Rahardian Putra Andi Kurniawan Anjasmara, Aang Setyawan Apriliyanti, Fisma Josara Aprillia Mifthakun Ni’mah Mifthakun Ni’mah Ni’mah Arief Andy Soebroto Arief Darmawan Arief Darmawan Awalia Oktaviana Ayu Winna Ramadhani Azhar, Alby Ghifari Baghaz Dzulhamdhani Surya Putra, Renanda Baghaz, Renanda DSP Bagyo Yanuwiadi Bagyo Yanuwiadi Bimo Aji Nugroho Bimo Aji Nugroho Catur Budi Noviya Destrina Elita Koto Devi Rahmania Nureka Wasti Dewi Nur Setyorini Diana Arfiati Dini Anggraini Dini Sofarini Dyah Ayu Wijayanti Eka Supriatin, Febriyani Endang Yuli Herawati Ertikasari, Nuning Eryanto, Erik Evellin Dewi Lusiana Evellin Dewi Lusiana, Evellin Dewi Faizal Kasim Farikhah Farikhah, Farikhah Fitrianesia, Fika Hafish Gigih Purnama Halimah, Marsa Fatin Handoko, Agung Dwi Happy Nursyam Herawati Umi S. Heru Suryanto Ilham Afandy Afandy Irawati Mei Widiastuti Islamy, R Adharyan Ismail, Azwar Ismail, Mahrus Iswati Iswati Ita Fitria S Jatayu, Diklawati Jati Batoro Jibril Maulana Kasyful Amron Kurnianingsih, Asmiyati Lal, Mohammad Tamrin Bin Mohamad Latifa, Yusria Listiya Gita Lesmana Made Dwipa Kusuma Maharani Maftuch Maftuch Masitha, Amira Miftahul Khair Kadim Moh. Wildanul Jannah MOHAMAD FADJAR Mohammad Mahmudi Muhammad Arifin Gymnastyar Muhammad Dailami, Muhammad Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Musa Muhammad Sumsanto Mukhlis, Dharu Habib Neni Diah Kusumaning Arum Nico Rahman Caesar Ningsih, Hartati Kartika Nugroho, Bimo Aji Nurul Mutmainnah, Nurul Paricahya, Akhsan Fikrillah Patresna, Laelatul Nisa Prananda, Nauval Rifqi Prasetyawati, Ranita Ayu Punto Apri Sembodo Putra, Andhika Farras Rahardian Putra, Renanda Baghaz D.S. Putra, Renanda Baghaz Dzulhamdani Surya Putra, Renanda Baghaz Dzulhamdhani Surya Qurniawatri, Agustiansi Yeyen Rahardjo, Seto Sugianto Prabowo Rahmadia, Alvira Rizqi Rahmi Nurdiani Ranita Ayu Prasetyawati Renanda Baghaz Dzulhamdhani Surya Putra Renanda Baghaz Dzulhamdhani Surya Putra Rizka Rahmana Putri Savira, Riza Sembodo, Punto Apri setya widi ayuning permanasari Shiddiq, Muhammad Faiq Ash Shofiyatul Lailiyah Shofwan, Shofwan Sisca Fajriani Sri Andayani Sri Astutik Sujono Sujono Supriatin, Febriyani Eka Supriatna . Supriatna Supriatna Supriyatin, Febriyani Eka Sutrisno, Bagas Gagat Rahina Asihing TIWI NURJANNATI UTAMI Utari, Inpita Casuarina Eqisetia Uzair, Hafiz Muhammad Wahidah Inayati Wijanata, Ida Bagus Pascad Wijayanti, Dyah Ayu Yanu Iswahfiudin Yanuhar, Uun Yenny Risjani Yudhistira, Ikmal Fahmi Yuni Kilawati Yuniari, Shinta Hiflina Yunita Maimunah Zahrotur Rosyidah