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All Journal Jurnal Veteriner Media Kedokteran Hewan Jurnal Medik Veteriner JURNAL KAJIAN VETERINER Ovozoa: Journal of Animal Reproduction Journal of Applied Veterinary Science and Technology journal of Basic Medical Veterinary Journal of Fisheries & Marine
Widiyatno, Thomas Valentinus
Departemen Patologi Veteriner, Fakultas Kedokteran Hewan, Universitas Airlangga
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Journal : journal of Basic Medical Veterinary

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PENGARUH PEMBERIAN EKSTRAK SAMBILOTO (Andrographis Paniculata) TERHADAP GAMBARAN HISTOPATOLOGI GINJAL YANG DIINDUKSI GENTAMISIN PADA TIKUS (Rattus norvegicus) Nugrahani, Anggi Dwi; Nangoi, Lianny; Meles, Dewa Ketut; Widiyatno, Thomas Valentinus; Hamid, Iwan Sahrial; Santoso, Kuncoro Puguh
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19989

Abstract

This research was aimed to know the effect of leaves extract of sambiloto as a nephroprotector on renal histopathology features induced by gentamicin in white rats (Rattus norvegicus). The effect of leaves extract of sambiloto was tested in 25 rats. Twenty five male white rat were randomly divided into five groups; K- as negative control was given carboxy methyl cellulosa in 15 days, P0 was given carboxyl methyl cellulosa in 15 days, P1 was given leaves extract of sambiloto of 177 mg/kg bw/day/po in 15 days, P2 was given leaves extract of sambiloto of 189 mg/kg bw/day/po in 15 days, P3 was given leaves extract of sambiloto of 239,5 mg/kg bw/day/po in 15 days. On the 15 day of experimental, P0, P1, P2, P3 was given 200 mg/kg bw of gentamicin. Gentamicin solutions on P0, P1, P2 and P3 treated through intramuscular injection. After 24 hours of gentamicin induced, take the rats renal by laparotomy and then making preparations histopathology with staining Haematoxylin-Eosin (HE). Based on the result of Kruskall-Wallis statistical analysis on renal histopathology showed this research that the treatment have significant difference (p < 0,05). The leaves extract of sambiloto treated group (P3) gave the best value on the renal morfology, it gave a good effects in preventing nephrotoxicity in white rats.
PENGUJIAN VAKSIN HEPATITIS B FASE SUBKRONIS TERHADAP BERAT ORGAN DAN DIAMETER PULPA PUTIH LIMPA TIKUS PUTIH (Rattus norvegicus) Anindya, Afifatus; Santoso, Kuncoro Puguh; Rantam, Fedik Abdul; Rachmawati, Kadek; Nidom, Chairul Anwar; Widiyatno, Thomas Valentinus; Plumeriastuti, Hani
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20410

Abstract

This study aims to determine the effect of hepatitis B vaccine of spleen weight and white pulp diameter in rat (Rattus norvegicus). This study used 20 male wistar rats aged 8 weeks with an average weight of 200-250 grams, which was divided into four groups. Control group (P0) was given PBS solution, treatment group 1 (P1) was given type 1 hepatitis B vaccine, treatment group 2 was given type 2 hepatitis B vaccine, and treatment group 3 (P3) was given type 3 hepatitis B vaccine. Dose administered 0.4 ml and given repeatedly at days 0,30, and 60. Animals model to euthanize on day 75 for sampling. The spleen organ is weighed after the necropsing process and then the spleen is stored for the preparation of histopathologic with HE staining (Hematoxylin Eosin). The data obtained are weight data of spleen organ and spleen white pulp diameter. The results showed the highest weight of spleen organ 0.92 gram in group P0 and the largest white pulp diameter 34,34 μm in group P1. The results of data analyzed using one-way ANOVA (Analysis of Variance) and continued with Duncan alpha test. One Way Anova and Duncan alpha test showed that there were non significantly different between groups (p>0.05). The conclusion is we know that hepatitis B vaccine type 1,2, and 3 can't give the effect for spleen weight and white pulp diameter in rat (Rattus norvegicus).
THE EFFECT OF POLYPROPYLENE PLASTIC RESIDUE ON HEATED PALM OIL AGAINST THE HISTOPATHOLOGICAL CHANGES OF KIDNEY ON MALE RATS (Rattus norvegicus) Cahyono, Shahani Azpriyanne; Eliyani, Hana; Widiyatno, Thomas Valentinus; Plumeriastuti, Hani; Hidajati, Nove; Santoso, Kuncoro Puguh
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20415

Abstract

This research was conducted to observe the effect of polypropylene plastic residue on heated palm oil against the histopathological changes on rat's kidney. There were 18 male rats (Rattus norvegicus) that used as the examination animal and were divided into three groups and were given the mixture of heated palm oil and polypropylene plastic per oral with intubation needle. The treatment was done for five weeks. The treatment for P0 group was 84 g of palm oil, for P1 was 84 g of palm oil with 2.3 g of plastic, and for the P2 was 84 g of palm oil with 6.8 g of plastic. The histopathological changes of the kidney were observed using microscope with 400x magnification and the data was analysed using Kruskal-Wallis and continued with Mann Whitney U test. The result showed that there were significant differences between three groups of treatment (P<0.05). There were effects of polypropylene plastic residue on heated palm oil against the histopathological changes of kidney in this research such as cast, epithelial sloughing and necrosis in the mild scale.
GAMBARAN PATOLOGI HEPAR IKAN LELE DUMBO (Clarias gariepinus) YANG DIPAPAR LOGAM TIMBAL NITRAT Pb(NO3)2 Dellavia, Yolanda; Widiyatno, Thomas Valentinus; Setiawan, Boedi; Arimbi, Arimbi; Poernomo, Bambang; Dhamayanti, Yeni
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21092

Abstract

This research aimed to know the changed damage of liver African catfish (Clarias gariepinus) were exposed by lead nitrat. This study used twenty four of African catfish (Clarias gariepinus) with average weight of 20-25 grams, size 10-12 cm, age ± two months. This research was designed by a completely randomized design (CRD). All member of population of the African catfish were divided into six groups, consist of six repetitions each, namely P1, P2, P3, and P0 as a negative control respectively.  P1 was given with dosage of lead nitrat 7,26 mg/liter, P2 was given with dosage of lead nitrat 14,53 mg/liter, and P3 was given with dosage of lead nitrat 29,06 mg/liter. According to the macroscopic observed, show that liver became swollen and pale.  The histopathological features of hepar were examined under light microscope in 400 times magnification. Scoring method were using Bernet Scoring Method to examined the presence of degeneretion, congesti, necrotic, and infiltration of leukocyte. Then, Kruskal-Wallis test through with Mann-Whitney test of statistical analysis. The statistical analysis showed the median number of P0 (0,05), P1 (0,45), P2 (1,10), and P3 (1,40) respectively. From the result can be concluded lead exposure with dose 29,06 mg/liter severe which was heavily histopatological in hepatocytes cell of african catfish (Clarias gariepinus) liver.
Taurine Antioxidant Effect in Decreasing Kidney Damage in Male Mice (Mus Musculus) due to Oxidative Stress Induced by Paraquat Pratama, Ricki; Hestianah, Eka Pramyrtha; Widiyatno, Thomas Valentinus; Meles, Dewa Ketut; Kurnijasanti, Rochmah
Journal of Basic Medical Veterinary Vol. 10 No. 2 (2021): Journal of Basic Medical Veterinary, December 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i2.31811

Abstract

Paraquat toxicity occurs through the high production of reactive oxygen species (ROS) which cause damage due to oxidative stress. Antioxidants can reduce damage through prevention of oxidative stress. Taurine has shown the ability to act as an antioxidant. The aim of this research is to find a effect of antioxidant taurine to decrease kidney damage caused by oxidative stress due to paraquat by looking at the histopathology changes. 25 male adult mices from strain DDY were used and divided into five treatment groups; C(-) (Aquadest IP), C(+) (Paraquat 30mg/kg), P1 (Paraquat 30mg/kg + Taurine 250mg/kg), P2 (Paraquat 30mg/kg + Taurine 500mg/kg) and P3 (Paraquat 30mg/kg + Taurine 1000mg/kg). All groups were given treatment intraperitoneal for twenty one days. The mices were sacrificed where kidney were collected for histopathology preparation. The parameters measured were renal histopathological changes in form of degeneration and necrosis. The results show that taurine administration had an effect on decreased degrees of damage to kidney tubular cells, with a decrease in the mean degree of renal tubular degeneration and necrosis. Degeneration of renal tubular cells in groups (P2) reduced compared to the group (C+) there were significant differences (P <0.05). Necrosis of renal tubular cells in groups (P1, P2) reduced compared to the group (C+) there were significant differences (P <0.05).  In conclusion, this research proves that administration of paraquat causes renal histopathological changes which are characterized by degeneration and necrosis. It also proves that taurine dose of 500 mg/kgBB could provided optimal effect.
The Effect of Red Ginger Extract (Zingiber officinale var rubrum) on The Thickness of The Epitelial and Diameter of Seminiferous Tubules in Albino Rat (Rattus norvegicus) Exposed Monosodium Glutamat Renaldi, Nogi; Rachmawati, Kadek; Widiyatno, Thomas Valentinus; Madyawati, Sri Pantja; Dhamayanti, Yeni
Journal of Basic Medical Veterinary Vol. 13 No. 1 (2024): Journal of Basic Medical Veterinary, June 2024
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v13i1.51158

Abstract

Background: This study examined administration of MSG intraperitoneally to white rats resulted in a decrease in testicular weight, an increase in lipid peroxidase levels The testes, as the site of spermatogenesis, are highly vulnerable to oxidation processes caused by free  radicals,  which  can  disrupt spermatogenesis. Free radicals can be countered by antioxidants. One plant believed to have antioxidant activity is red ginger. Purpose: To determine the effect of red ginger against histopathology seminiferous tubules of white rat (Rattus norvegicus) exposed by monosodium glutamate. Methods: This study used 30 rats which were divided into 5 groups. The positve control group (K+) were given only monosodium glutamate 5g/kgBW and CMC Na orally, the treatments groups P1 were given monosodium glutamte 5g/KgBW + red ginger 50mg/rat/day, P2 were given 5g/KgBW + red ginger 100mg/rat/day, and P3 were given 5g/KgBW + red ginger 200mg/rat/day. All treatments were administered for 42 days. After 42 days of treatments, all rats were sacrificed and seminiferous tubules were observed with microscope from pathology laboratory. Results: Administration of red ginger extract can defend seminiferous tubules epithelial thicknes and diameter from exposure of monosodium glutamate with 100mg/KgBW dosage as the most effective dosage. Seminiferous tubules epitelial thickness and diameter were tested using one-way ANOVA and Duncan's multiple range test. Conclusion: Red Ginger Extract (Zingiber officinale var rubrum) has shown potential effects to counteract Monosodium Glutamat consequences on The Thickness of the epitelial and diameter of seminiferous tubules of white rat (Rattus norvegicus).
Co-Authors Abdul Samik, Abdul Aksono HP., Eduardus Bimo Amirotul Azhimah Anindya, Afifatus Arianto, Intan Diah Safitri Arief Budiman Arimbi Bambang Poernomo Bambang Sektiari Lukiswanto Boedi Setiawan Cahyono, Shahani Azpriyanne Dellavia, Yolanda Eka Pramyrtha Hestianah, Eka Pramyrtha Eko Agus Suyono Fedik Abdul Rantam Hana Eliyani Hani Plumeriastuti Hestiana, Eka Pramyrtha Hidajati, Nove Hidayatik, Nanik Hisyam, Mirza Atikah Madarina Ira Sari Yudaniayanti Iwan Sahrial Hamid Kadek Rachmawati Khairullah, Aswin Rafif Kuncorojakti, Suryo Lastoaji, Firman Lianny Nangoi Lilik Maslachah Lovela, Aprinda Ratna Lucia Tri Suwanti, Lucia Tri Ma'ruf, Anwar Mahendra Pujiyanto Meles, Dewa Ketut Mochamad Donny Koerniawan Nidom, Chairul Anwar Nugrahani, Anggi Dwi Nunuk Dyah Retno Lastuti Panggabean, Rosenna Tiara Poedji Hastutiek Prambudi, Dhimas Toni Angger Pratama, Ricki Putri, Mirtanti Kiswandi Renaldi, Nogi Rochmah Kurnijasanti Santoso, Kuncoro Puguh Sri Pantja Madyawati, Sri Pantja Sukmanadi, Mohammad Theresia, Sis Wisnuarti Ulfah Juniarti Siregar Wiwik Misaco Yuniarti Yeni Dhamayanti Yusuf, Anang Zhaza Afililla