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Yunita Rusidah
Universitas Muhammadiyah Kudus
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Parijoto (Medinilla speciosa) extract as a natural dye alternative for peripheral blood smears: A comparative study with giemsa stain Trisna, Meida; Indanah, Indanah; Rusidah, Yunita; Saputro, Arief Adi; Sholikhati, Anisa; Ekawati, Sherly Nur
Journal of Indonesian Medical Laboratory and Science Vol 7 No 1: April 2026
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

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Abstract

Background: Peripheral blood smear staining is an essential hematological procedure used to evaluate blood cell morphology. The commonly used standard stain is Giemsa stain; however, its chemical components may pose potential risks to health and the environment. Therefore, safer natural alternatives are needed. Previous studies have reported that anthocyanin-containing plant extracts, such as Hibiscus sabdariffa, Tectona grandis, and Garcinia mangostana, can effectively stain blood cells. Medinilla speciosa (parijoto) also contains anthocyanins and has potential as a natural dye. Objective: This study aimed to evaluate the staining quality of peripheral blood smears using Medinilla speciosa extract at different concentrations and to compare the results with a 10% Giemsa stain control. Methods: This laboratory-based experimental study used a post-test only control group design. A total of 30 blood smears were stained using Medinilla speciosa extract at concentrations of 25%, 50%, and 70%, along with a 10% Giemsa stain control. The extract was prepared using maceration with pH adjustment to optimize anthocyanin stability. Staining quality was assessed based on color intensity, background clarity, and erythrocyte morphology using a semi-quantitative scoring system. Data were analyzed using the Kruskal–Wallis test and Mann–Whitney U test. Results: Increasing extract concentration was generally associated with improved staining quality. A significant difference among groups was observed (p = 0.002). Pairwise analysis showed that the 70% extract was not significantly different from the 10% Giemsa stain control (p = 0.321), while lower concentrations differed significantly. Conclusion: The 70% Medinilla speciosa extract showed the best performance among extract groups and demonstrated comparable staining quality in certain parameters. However, Giemsa stain remains the more consistent standard. Further optimization is required before routine application.
HEALTH COUNSELING TO INCREASING KNOWLEDGE AND COMPLIANCE IN HYPERTENSION PATIENTS Fanani, Zaenal; Rusidah, Yunita; Kurnia, Shinta Dwi; Farikhah, Lailatul; Munandiyah, Munandiyah
(IJP) Indonesia Jurnal Perawat Vol 10, No 2 (2025): INDONESIA JURNAL PERAWAT
Publisher : Universitas Muhammadiyah Kudus

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26751/ijp.v10i2.2674

Abstract

Hypertension is a silent killer because it often occurs without complaints, symptoms can vary among individuals, and are almost the same as the symptoms of other diseases and can cause several complications including coronary heart disease, stroke and kidney failure. To support appropriate hypertension therapy, health counseling is needed given that knowledge and adherence to the expected therapeutic effect can be achieved. The purpose of this study was to determine the influence of health counseling on increasing patient knowledge and compliance. Primary data collection was carried out from February to March 2023, using the one-group pre and post-test experimental quasi-methods, for 55 respondents. The research instrument used two questionnaires, namely patient knowledge and compliance. Blood pressure was 140/90 in 34 (61,82%) patients and the duration of hypertension was 5 years in as many as 43 (78,2%) patients. The percentage of patients who completed the post-test patient knowledge was as high as 100%, and the percentage of patients who completed the post-test compliance was as high as 63,64%. A significant difference was found between the two data sets (p= 0.000). Patient knowledge and compliance increased significantly from before health counseling to after health counseling.
Co-Authors Anisa Sholikhati Anisa Sholikhati, Anisa Anita Afrianingsih Anjani, Anisa Ayu Annisya Luthfi Septanti Anny Rosiana Aprilia Sugiri Arief Adi Saputro Arina Lis Sa'adah Avira Budianita Bintari Tri Sukoharjanti Budianita, Avira Dewi Hartinah Dewi Nur Wakhidah Diah Putri Nugraheni Diana Tri Lestari Dina Amalia Ekawati, Sherly Nur Endang Setyowati Fariza Yulia Kartika Sari Fariza Yulia Kartikasari Fathimah Isnina Indanah Islami Islami Ismi Izzatul Maula Ismoyowati Ismoyowati Kurniawati, Eka Lailatul Farikhah Lailatul Farikhah Lailatul Farikhah, Lailatul Munandiyah, Munandiyah Nirmala Manik Nurul huda Okta Yosiana Dewi Qurrotu A'yuni Auliya Ridwanto, Muhammad Sa’adah, Arina Lis Septiani Septiani Shinta Dwi Kurnia Sukarmin Sukarmin Sukoharjanti, Bintari Tri Suwarto Suwarto Tanaya, Nindy Trisna, Meida Ulfa, Navira Septiana Yayuk Mundriyastutik Yulia Sistina Yunus Mustaqim, Yunus Zaenal Fanani Zamroni, Aini Rizka Amalia