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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Biospecies METAMORFOSA Journal of Biological Sciences Reaktor Bioma : Berkala Ilmiah Biologi Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology AL KAUNIYAH Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Bahan Alam Terbarukan Biosfer: Jurnal Tadris Biologi Biogenesis: Jurnal Ilmiah Biologi Journal of Degraded and Mining Lands Management Indonesian Journal of Biotechnology Jurnal Agro Planta Tropika BIO-SITE |BIOLOGI Sains Terapan Majalah Obat Tradisional Journal of Natural Sciences and Mathematics Research Jurnal Perlindungan Tanaman Indonesia Journal of Tropical Biodiversity and Biotechnology Omni-Akuatika Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Jurnal Agrotek Tropika Jurnal Riset Biologi dan Aplikasinya Berkala Ilmiah Biologi International Journal of Oil Palm
Rina Sri Kasiamdari
Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Decision Sciences, Operations Research & Management Education Energy Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Masa Inkubasi Gejala Penyakit Hawar Daun Tanaman Kentang yang Diinduksi Ketahanannya oleh Jamur Antagonis Trichoderma viride Purwantisari, Susiana; Priyatmojo, Achmadi; Sancayaningsih, Retno Peni; Kasiamdari, Rina Sri
Bioma : Berkala Ilmiah Biologi Vol. 18, No.1, Tahun 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (62.469 KB) | DOI: 10.14710/bioma.18.2.41-47

Abstract

Late blight disease caused by Phytophthora infestans, is probably the single most important disease of potatoes worldwide. Infected plants were quickly killed and were difficult for replanting, causing significant losses for the growers. Various control methods were examined including the use of biocontrol agents of Trichoderma spp.  The biocontrol potential of Trichoderma viride against potato late blight pathogen, Phytophthora infestans, were studied  under greenhouse conditions. The research objective was to determine the ability of biocontrol agents Trichoderma viride to delay late blight disease incidence on potato plants in the field. The in vivo experiment was carried out at potato land area located at BALITSA in Cikole Sub District, Lembang District and Bandung Regency. Completely Randomized Design was used with six treatment and five replicates. The treatment tested consist of control (without pathogen fungal and antagonists fungus application too), control (with pathogen fungal application and without antagonist fungal application), chemical fungicide application, antagonist fungal application 2 weeks before planting, application 1 week after planting and both application 2 weeks before and 1 week after planting. Result of the research showed that application of antagonists fungal could delay disease intensity until 14 days. These antagonist could be used as biological agents initials to control leaf blight disease. Key words: Biological control, Potato late blight, Phytophthora infestans, Trichoderma viride
PRODUKSI INOKULUM VESIKULAR ARBUSKULAR MIKORIZA PADA INANG SORGHUM BICOLOR (L.) MOENCH DENGAN VARIASI JENIS INOKULUM DAN PUPUK NPK Auli, Nisa Raudatul; Kasiamdari, Rina Sri
Jurnal Riset Biologi dan Aplikasinya Vol 1, No 2 (2019)
Publisher : Universitas Negeri Surabaya

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Abstract

Penelitian ini bertujuan untuk menentukan kombinasi antara jenis inokulum Vesikular Arbuskular Mikoriza (VAM), kandungan NPK, dan umur tanaman yang paling efektif dalam produksi inokulum. Tanaman inang menggunakan Sorghum bicolor yang merupakan anggota dari famili Poaceae yang memiliki sistem perakaran yang luas, jumlah akar banyak, dan tumbuh dengan cepat sehingga berpotensi sebagai inang VAM yang kompatibel. Penelitian ini menggunakan analisis Rancangan Acak Lengkap (RAL) dengan faktorial 2x2x2 meliputi jenis mikoriza yang digunakan yaitu Glomus aggregatum (G) dan campuran Glomus aggregatum dan Gigaspora margarita (C). Konsentrasi NPK, yaitu NPK grower 15-9-20 (P9) dan Growmore 20-20-20 (P20). Umur tanaman 6 minggu dan 9 minggu. Parameter pertumbuhan yang diukur meliputi tinggi tanaman, berat kering batang dan akar, persen kolonisasi dan jumlah spora VAM. Berdasarkan penelitian didapatkan hasil, tanaman dengan inokulum campuran VAM dengan P20 menghasilkan tinggi tanaman dan berat kering batang dan akar tertinggi. Inokulum campuran mempunyai persen kolonisasi dengan nilai yang paling tinggi, sedangkan inokulum tunggal (Glomus aggregatum) dengan P rendah menghasilkan produksi spora paling tinggi. Inokulum campuran VAM dan P9 lebih baik dalam produksi inokulum, baik berupa akar terkolonisasi maupun spora.
DETECTION AND IDENTIFICATION OF POLYMORPHISM IN MUTANT STRAWBERRY (FRAGARIA SPP.) PLANTS BASED ON CLEAVED AMPLIFIED POLYMORPHIC SEQUENCES MOLECULAR MARKERS Aristya, Ganies Riza; Ayundai, Melin; Putri, Fauzana; Widiastuti, Ani; Kasiamdari, Rina Sri
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 8, No 1 (2020)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.2020.109.15-20

Abstract

Strawberry (Fragaria spp.) has a high economic value and various benefits, but the production of strawberry plants in Indonesia is still low in terms of both quantity and quality. Strawberry plant breeding can be done in various techniques, one of which is polyploidization. Polyploidization by an induction of colchicine at various concentrations in strawberry crops cv. California and Festival resulted in superior phenotype characteristics. To prove the existence of a change in ploidy in strawberry, then research at the molecular level needs to be done. The purposes of this study were to find out changes in ploidy of strawberry plants using CAPS molecular markers and to detect the polymorphism in strawberry plants quickly. The samples used were young leaves. Main procedure was the cutting of the amplified DNA using restriction enzymes of TaqI and HaeIII. The results showed that CAPS molecular markers were capable of detecting polymorphism quickly and efficiently in strawberry plants. Specific bands among strawberry plants having undergone polyploidization and those not having undergone polyploidization can be seen on the differences in monomorphic or polymorphic bands between the control plants and treated plants. 
Detection and Identification of Polymorphism in Mutant Strawberry (Fragaria spp.) Plants Based on Cleaved Amplified Polymorphic Sequences Molecular Markers Aristya, Ganies Riza; Ayundai, Melin; Putri, Fauzana; Widiastuti, Ani; Kasiamdari, Rina Sri
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 8, No 1 (2020)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.2020.109.15-20

Abstract

Strawberry (Fragaria spp.) has a high economic value and various benefits, but the production of strawberry plants in Indonesia is still low in terms of both quantity and quality. Strawberry plant breeding can be done in various techniques, one of which is polyploidization. Polyploidization by an induction of colchicine at various concentrations in strawberry crops cv. California and Festival resulted in superior phenotype characteristics. To prove the existence of a change in ploidy in strawberry, then research at the molecular level needs to be done. The purposes of this study were to find out changes in ploidy of strawberry plants using CAPS molecular markers and to detect the polymorphism in strawberry plants quickly. The samples used were young leaves. Main procedure was the cutting of the amplified DNA using restriction enzymes of TaqI and HaeIII. The results showed that CAPS molecular markers were capable of detecting polymorphism quickly and efficiently in strawberry plants. Specific bands among strawberry plants having undergone polyploidization and those not having undergone polyploidization can be seen on the differences in monomorphic or polymorphic bands between the control plants and treated plants. 
Identifikasi dan Uji Patogenisitas Colletotrichum spp. dari Cabai Merah (Capsicum annuum): Kasus di Kricaan, Magelang, Jawa Tengah Noorkomala Sari; Rina Sri Kasiamdari
Jurnal Ilmu Pertanian Indonesia Vol. 26 No. 2 (2021): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.26.2.243

Abstract

The investigation of the agent causing diseases is the first step to determine and control the spread of diseases. Anthracnose causing fruit rot on the red pepper in Indonesia that reduces the yield up to 90%. Colletotrichum is reported as the causative fungal agent of anthracnosis on the red pepper. This study aims to determine the type of Colletotrichum spp. pathogenic fungi on the red pepper collected from Agricultural Field Kricaan, Magelang, Central Java. Methods of identification included macroscopic and microscopic characterization and followed by determining the pathogenicity of the pathogenic agent. In this study, Colletotrichum gloeosporioides and C. acutatum are identified as the causative agents of anthracnose in the red pepper collected from the study location. C. acutatum PC3 strain is identified as the most virulent pathogenic agent. Keywords: anthracnosis, diagnosis, diseases, symptoms, pathogen
Penapisan Cendawan Trichoderma spp. untuk Pengendalian Phytophthora infestans secara in vitro Susiana Purwantisari; Achmadi Priyatmojo; Retno Peni Sancayaningsih; Rina Sri Kasiamdari
Jurnal Fitopatologi Indonesia Vol 12 No 3 (2016)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (329.348 KB) | DOI: 10.14692/jfi.12.3.96

Abstract

Late blight disease on potato caused by a plant pathogenic fungus (Phytophthora infestans)  is the most important disease in Indonesia.  The use of antagonist fungi Trichoderma is an environmentally friendly technology to control the potato disease. The purpose of this study was to determine the ability of Trichoderma spp. to control P. infestans in vitro. Trichoderma spp. have been isolated from suppressive soil at central potato areas in district of Lembang, Bandung, West Java Province. To determine the inhibition ability of Trichoderma spp against P. infestans, a dual culture method was performed.  Variable observed was inhibition zone of Trichoderma spp. against P. infestans. The results showed that 9 isolates of Trichoderma were successfuly isolated from suppressive soil, i.e. T. viride (2 isolates), T. atroviride (1 isolate), T. harzianum (1 isolate) and T. aureoviride (5 isolates). All the Trichoderma isolates revealed growth inhibition abality against P. infestans and. The highest growth inhibition (68.6%) was observed by T. viride isolate ( Ti 9).
Bioremediation of Indigosol Blue 04B Batik Effluent by Indigenous Fungal Isolates, Aspergillus spp. Ratna Stia Dewi; Rina Sri Kasiamdari; Erni Martani; Yekti Asih Purwestri
Journal Omni-Akuatika Omni-Akuatika Special Issue 2nd Kripik SCiFiMaS
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (555.047 KB) | DOI: 10.20884/1.oa.2018.14.2.537

Abstract

Effluent from the local batik home industry is a serious problem, because the effluent discharge generated is spread in different places. Untreated effluent can cause environmental pollution, such as in groundwater reservoirs,because most is discharged into rivers. The aim of this research was to evaluate the bioremediation potential of indigenous fungi in liquid culture media with Indigosol Blue 04B (IB) batik effluent. The fungi isolates tested were Aspergillus sp. 1, Aspergillus sp. 2 and Aspergillus sp. 3, isolated from dye effluent soil and batik effluent, and compared to white rot fungi (Phanerochaete chrysosporium) as a positive control.   The physiochemical properties of IB batik effluent before and after fungal treatment were investigated. All of these parameters before the fungal treatment were above the recommended standard values based on the Governor regulation of Yogyakarta Special Region No. 7/2010. The level of biochemical oxygen demand (BOD), chemical oxygen demand (COD), total dissolved solids (TDS), total suspended solids (TSS), and electrical conductance (EC) was reduce by Aspergillus spp. The highest percentage reduction was achieved by Aspergillus sp. 3, namely 88.34% BOD, 89.11% COD, 75.77% TSS, 85.85% TDS and 71.21% EC, after 3 days of incubation. These results show that the positive control isolate had the lowest value. The study confirms the ability of indigenous fungi isolates in the remediation of IB batik effluent and their potential for future analysis in the treatment of all types of batik effluent.
Karakterisasi Morfologi dan Anatomi Stroberi (Fragaria x ananassa D. cv. Festival) Hasil Induksi Kolkisin Ana Susianti; Ganies Riza Aristya; Sutikno S; Rina Sri Kasiamdari
Biogenesis: Jurnal Ilmiah Biologi Vol 3 No 2 (2015)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v3i2.929

Abstract

Strawberry is a plant cultivated in subtropical countries, while Indonesia’s tropical climate. This is not accordance with the conditional of the natural growth of strawberries. Strawberries cultivar Festival is one of holticulture products in society. The high rates of consumption of strawberries are not equal with production and quality of strawberries. The quality of strawberries were determined by fruit size and uniformity of fruit. Effort that can be used to improve the qualityof strawberry production is polyploiditation with mutagenic substance, example colchicine. Result of polyploidy in morphological character are bigger. Technique of polyploidy with colchicine will be success if the number and structure of chromosom in cell nucleus are changes. Chromosome number changes will be change to anatomy, morphology and physiology plants. The aims of this research were to know the differences of anatomical and morphological characters of strawberry cultivar Festival after colchicine induction and find out the influence of the colchicine concentration differences in morphological and anatomical character of strawberry cultivars Festival. The research was have two main stages. The first were observation and scoring of 25 morphological characters of strawberries after colchicine induction with 0%; 0,01% and 0,05%. The second, preparations with paraffin method on the root, steam, leaves and fruit of the strawberry plant after colchicine induction. The observation of anatomical characters are measurements number cell was from of the root, steam, leaves and fruits. The results showed that induction of colchicine in strawberry cultivars festival showed different morphological characters on the density of the leaves, number of stems, fruit ripening time, fruit size, fruit sweetness of the outer color of the fruit, fruit acidity compared to control plants. Anatomical characters of cell size increases the cortex and pith of stems, leaves, cell mesofil cells of the cortex and pith of fruit. Colchicine induction of 0.05% was more effective in increasing the size of the fruit while the induction of colchicine of 0.01% was more effective at increasing the size of vegetative organs of strawberry cultivars Festival plants.Keywords: anatomy, colchicine, Festival Cultivars, morphology, strawberry
Uji Antagonisme Tiga Isolat Fungi Endofit Anggrek Bulan Terhadap F. Oxysporum Secara in vitro Khaterine K; Rina Sri Kasiamdari
Biogenesis: Jurnal Ilmiah Biologi Vol 4 No 1 (2016)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v4i1.1120

Abstract

Phalaenopsis amabilis (L.) Blume or Moth Orchid is Indonesia’s native orchid. In its cultivation, the crown rot which is caused by F. oxysporum is one of diseases that usually attack P. amabilis. The aim of this research is to assess the potency of endophytic fungi Sistotrema sp., Ceratorhiza sp. and Moniliopsis sp., that were isolated from healthy P. amabilis roots, in inhibiting F. oxysporum growth in vitro. The experiment of antagonist test was arranged in complete randomized design by three replications. The in vitro antagonist test was done on PDA and the parameter was the percentage inhibitation of F. oxysporum growth by endophytic fungi. The collected data were analyzed by one way anova and DMRT. The result showed that the three endophytic fungi isolated from P. amabilis roots had the potency in inhibiting F. oxysporum. In vitro antagonism test result showed that Moniliopsis sp. had the highest inhibition percentage (71,80%) among Ceratorhiza sp. (67,70%) and Sistotrema sp. (54,87%).
The growth response of pokem (Setaria italica L.) inoculated with arbuscular mycorrhizal fungi (AMF) from tailings area Suharno Suharno; Retno Peni Sancayaningsih; Endang Sutariningsih Soetarto; Rina Sri Kasiamdari
Journal of Degraded and Mining Lands Management Vol 8, No 4 (2021)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2021.084.2873

Abstract

The purpose of this research was to study the growth response of pokem (Setaria italica), which was inoculated by the arbuscular mycorrhizal fungi (AMF) from the tailings area. The method used in this research was a completely randomized design of factorial pattern. The factors consisted of AMF types (M)(M0: non-mycorrhizal, M1: Claroideoglomus etunicatum BGR, M2: C. lamellosum B1107S, M3: C. etunicatum L3101D), and inoculum density (I) (I: 5 g, II: 10 g per pot of planting media) with 8 replicates. The source of inoculum C. lamellosum B1107S and C. etunicatum L3101D originated from tailings in the gold mining area of Timika. The results showed that inoculation of C. etunicatum L3101D could increase the plant growth better than C. etunicatum BGR and C. lamellosum B1107S. Significant growth occurred on the parameters of the height of the plants, leaf area, dry weight and fresh weight of the plants, relative growth rate, and phosphorus absorption by the plants. The amount of nitrogen tended to decrease with AMF treatment, but it was not significant, whereas K has insiginificantly increased. The propagule density treatment increased plant growth on all parameters. The best growth occurred if the type C. etunicatum L3101D was inoculated with 10 g of propagule per planting media.
Co-Authors ', Padil AA Sudharmawan, AA Achmadi Priyatmojo Adiana Nayogyani Ali Djamhuri Ali, Nur Ayu Aisyah Ana Susianti Andang Syaifudin Ani Widiastuti, Ani Auli, Nisa Raudatul Ayundai, Melin Ayundai, Melin BUDI SETIADI DARYONO BUDI SETIADI DARYONO C. J. Soegihardjo C.J. Soegihardjo C.J. Soegihardjo C.J. SOEGIHARDJO Chasanah, Laila Uswatun Dhia Salsabila Hakim Dian Fitriarni Ekowati , Nuraeni Elzahra Nadya Putri Endang Sutariningsih Soetarto ENDANG SUTARININGSIH SOETARTO Erni Martani Erni Martani Febriani, Anya Via Ganies Riza Aristya Hasnaul Maritsa I Gusti Wayan Murjana Yasa Inayati, Evi Indra Sukmawati Junita Hardini Khaterine K Kristamtini Kristamtini, Kristamtini Kusrinah Kusrinah Kusrinah Kusrinah Laila Hanum Maulin Nafisa Muazam, Arif Muslikhin Hidayat Nita Aminasih Nuraeni Ekowati Nuraeni Ekowati Nuraeni Ekowati Nuraeni Ekowati, Nuraeni Nursamsi Pusposendjojo Nursamsi Pusposendjojo Nursamsi Pusposendjojo Nursamsi Pusposendjojo, Nursamsi Nursela, Dewi Padil, Padil Purnomo Purnomo Pusposendjojo , Nursamsi Putranto, Dwiyandito Ikhsan Putri, Elzahra Nadya Putri, Fauzana Putri, Fauzana R. Rugayah Ratna Stia Dewi Ratna Stia Dewi Reine Suci Wulandari Rejeki Siti Ferniah Retno Peni Sancayaningsih Retno Peni Sancayaningsih RETNO PENI SANCAYANINGSIH Ristiyani Khofifa Putri ROSA SURYANTINI Rugayah Rugayah S. Santosa Santosa . Santosa Santosa Santosa Santosa Sari, Noorkomala Setyorini Widyayanti, Setyorini Singgih Tri Wardana Siti Syamsiah Siti Syamsiah Soegihardjo , C.J. Soekarti Moeljopawiro Soekarti Moeljopawiro, Soekarti Suharno Suharno Susiana Purwantisari Sutikno S Syamsiah, Siti Widiastuti, Ani Yekti Asih Purwestri Yudhistira Nugraha