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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Biospecies METAMORFOSA Journal of Biological Sciences Reaktor Bioma : Berkala Ilmiah Biologi Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology AL KAUNIYAH Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Bahan Alam Terbarukan Biosfer: Jurnal Tadris Biologi Biogenesis: Jurnal Ilmiah Biologi Journal of Degraded and Mining Lands Management Indonesian Journal of Biotechnology Jurnal Agro Planta Tropika BIO-SITE |BIOLOGI Sains Terapan Majalah Obat Tradisional Journal of Natural Sciences and Mathematics Research Jurnal Perlindungan Tanaman Indonesia Journal of Tropical Biodiversity and Biotechnology Omni-Akuatika Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Jurnal Agrotek Tropika Jurnal Riset Biologi dan Aplikasinya Berkala Ilmiah Biologi International Journal of Oil Palm
Rina Sri Kasiamdari
Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Decision Sciences, Operations Research & Management Education Energy Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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DAYA ANTIMIKROBA METABOLIT BIOAKTIF JAMUR SHIITAKE (Lentinula edodes (Berk.) Pegler) YANG DIKULTUR PADA TIGA JENIS MEDIUM FERMENTASI Nuraeni Ekowati; Rina Sri Kasiamdari; Nursamsi Pusposendjojo; C.J. Soegihardjo
Majalah Obat Tradisional Vol 16, No 3 (2011)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (527.742 KB) | DOI: 10.22146/tradmedj.8050

Abstract

Shiitake mushroom (Lentinula edodes (Berk.) Pegler) is one of the fungi that has potentially been used as food and medicinal mushroom. Cultivation in liquid medium by the fermentation process has been developed over recent years. Four isolates of L.edodes (isolates from Malang, Cianjur, Lembang and Yogyakarta) cultured on three types of fermentation media (KM: Medium Kauffman; GYMT: Glucose, Yeast Extract, Malt Extract, Thiamin; YEMR: Yeast extract, Malt Extract, Rice bran). Bioactive metabolites  extracted from mycelium biomass and fermentation culture filtrates were tested using pathogenic microbial Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 35218, Candida albicans ATCC 10231, and Trichophyton mentagrophytes. Inhibition zone diameter data were analyzed using analysis of variance followed by Duncan test at 5% error rate. The results showed that bioactive compounds in the culture filtrates and mycelium biomass of L. edodes extracted using chloroform, ethyl acetate and water, were able to inhibit S. aureus, E. coli and C. albicans,  but were not able to inhibit T.mentagrophytes. Isolates of L.edodes from Lembang and Yogyakarta cultured on Kauffman Medium gave  the best results with inhibitory zones of 24.97 to 31.14 mm. Microbes which gave the most sensitive of bioactive compounds test from L. edodes were C. albicans followed by E. coli, S. aureus and T. mentagrophytes. Bioactive metabolites from L. edodes inhibited potentially pathogenic microbes  from the group of  bacteria and  yeasts but did not inhibit the fungus.
Morphological Characteristics and Kinship Relationship of Mushroom Schizophyllum commune Fr. Kusrinah Kusrinah; Rina Sri Kasiamdari
Journal of Natural Sciences and Mathematics Research Vol 1, No 2 (2015): December
Publisher : Faculty of Science and Technology, Universitas Islam Negeri Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (916.779 KB) | DOI: 10.21580/jnsmr.2015.1.2.1620

Abstract

Schizophyllum commune is a mushroom from the division of Basidiomycota which abundance at rainy season on wood that have been moldered for its habitat. S. commune has been distributed widely at various types of wood habitat (jack fruit, bamboo, rubber, mango etc). Existence of this mushroom has not been known as well as any mushroom are able to be consumed/edible yet. In Java, the societies who have known it as consumption mushroom called it with local name jamur gigit. Jamur gigit is potential to become food material and based on organoleptic test indicates that jamur gigit is more delicious from oyster mushroom. The purpose of research is to investigate the morphology character of S. commune at various growth phases and to study the S. commune relationship in Java. The result of this research is expected can give information about morphology character of S. commune in each its growth phase and to understand S. commune relationship in Java. Sample for research has been collected in 3 areas in Java. The macroscopic morphology character and microscopic character of S. commune were observed. The character that had been obtained is used to compile description and be analyzed by cluster using SPSS 13.0 to study the kinship relationship. The result of research indicates that morphology character of S. commune in Java of fruit body when old and young phase was different, while the microscopic character almost same to the obtained sample. The result of analysis cluster indicated that at similar level of 38,1 % there were two clusters, first cluster was Sidoarjo a S. commune and S. commune Yogyakarta. The second cluster has member the Sidoarjo b S. commune, Malang S. commune, Tangerang S. commune, Semarang S. commune and Kuningan S. commune. © 2015 JNSMR UIN Walisongo. All rights reserved.
Characterization and Pathogenicity of Rhizoctonia solani Kühn Associated with Sheath Blight in Local Rice Varieties (Oryza sativa L.) of Special Region of Yogyakarta Maulin Nafisa; Adiana Nayogyani; Rina Sri Kasiamdari
Jurnal Perlindungan Tanaman Indonesia Vol 25, No 2 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.67557

Abstract

In Indonesia, rice is used as the first strategic and priority food commodity in agricultural development. However, in practice, efforts to increase it still experience various obstacles, one of which is caused by the attack of pathogen Rhizoctonia solani. This study aimed to determine the characters of R. solani and observe its pathogenicity on some local rice varieties in the Special Region of Yogyakarta. Four isolates of R. solani were used, namely RS MS2, RS MKP2, RS UMB, and RS PN, as well as six varieties of local rice varieties, which were Menthik Susu, Cempo Merah, Cempo Hitam, Segreng, Menoreh, and Hitam Makaryo, and national rice varieties IR 64 and Ciherang. Morphological characterization was carried out based on the cultural characteristics of the colony, hyphae, and sclerotia. A pathogenicity test was carried out using the Detached Leaf Inoculation method. The result showed that R. solani characters vary from whitish-brown, yellowish-brown, and light brown colonies; moderate and fast growth rates; abundant-aerial, moderate-aerial, and slight-aerial growth patterns; 90° hyphae branching, narrowing of hyphae at the branching point, and presence of sclerotia. The pathogenicity test gave the results of all isolates having varying pathogenicity levels on each rice variety. Menthik Susu, Cempo Hitam, and Hitam Makaryo were resistant to R. solani. Cempo Merah and Segreng were considered as moderately resistant varieties, whereas Menoreh was categorized as moderately susceptible. Ciherang and IR64 were susceptible varieties to R. solani.
Isolation and Identification of Endophytic Fungi from Leave and Stem of Calopogonium mucunoides Dian Fitriarni; Rina Sri Kasiamdari
Journal of Tropical Biodiversity and Biotechnology Vol 3, No 1 (2018): April
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1392.455 KB) | DOI: 10.22146/jtbb.32477

Abstract

Thirty-seven isolates of endophytic fungi were isolated from leaves and stems of Calopogonium mucunoides collected from PTPN PTPN XII (Persero) Rubber Plantation, Klatakan, Kecamatan Tanggul, Kabupaten Jember, Jawa Timur. All isolates were identified based on morphological characteristics using the light microscope. The 37 isolates of endophytic fungi are members of Deuteromycota and Basidiomycota and classified to genera Phoma, Phomopsis, Corynespora, Rhizoctonia, Helicosporium, Curvularia, Torulomyces, Gliocladium, Gloeosporium, Acremonium, Tripospermum, Aureobasidium, Colletotrichum, Humicola, Fusarium, Sclerotium, and sterile hyphae.  
Thermostable Chicken Feather Degrading Enzymes from L-23 Isolate from Indonesia ROSA SURYANTINI; REINE SUCI WULANDARI; RINA SRI KASIAMDARI
Microbiology Indonesia Vol. 1 No. 3 (2007): December 2007
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5454/mi.1.3.3

Abstract

The thermostable chicken feather degrading protease enzymes used here was extracted and partially purified from thermophilic bacteria L-23 isolated from a coastal hot spring in North Sulawesi, Indonesia. The L-23 was grown in the selective medium containing 1% chicken feather powder at 70 °C and pH 7. The cell-free culture was precipitated with ammonium sulphate at 80% saturation, followed by heating at 65 °C for 1 h before applied onto Sephadex G-100. The molecular weight of the two enzymes identified were estimated as 47 and 64 kDa. The optimum pH of the mixed enzymes preparation was 7 while the optimum temperature was 65 °C. Zymogram analysis showed that one of the enzymes was still active after being heated at 100 °C for 20 min and was also resistant towards organic solvents and SDS. The activity was enhanced by addition of 1 mM FeCl3.
Pengaruh Inokulasi Mikoriza VA Terhadap Pertumbuhan Bawang Dayak (Eleutherine palmifolia (L.) Merr.) Pada Tanah Marginal Indra Sukmawati; Rina Sri Kasiamdari
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 38, No 1 (2021)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2021.38.1.1086

Abstract

Penggunaan jamur mikoriza pada lahan marginal menjadi usaha rehabilitasi kualitas tanah. Bawang Dayak (Eleutherine palmifolia (L.) Merr.) dipilih karena dapat bersimbiosis dengan fungi Glomus aggregatum untuk mendukung pertumbuhannya. Tujuan penelitian ini yaitu mengetahui peran dari VAM terhadap pertumbuhan bawang Dayak  dan kombinasi yang tepat untuk pertumbuhan bawang Dayak di media tanah marginal. Pada penelitian ini, tanaman ditumbuhkan selama tiga bulan di greenhouse. Parameter yang diteliti meliputi: tinggi tanaman, jumlah daun, berat kering (umbi, tajuk dan akar), jumlah umbi, presentase kolonisasi mikoriza, serta jumlah spora. Panen dilakukan 2 kali pada minggu ke-6 dan minggu ke-12. Pengamatan kolonisasi struktur mikoriza pada akar tanaman dilakukan melalui teknik pewarnaan akar (staining). Pengamatan spora dilakukan melalui metode wet sieving and decanting. Hasil dianalisis menggunakan Rancangan Acak Lengkap (RAL) dengan faktorial. Hasil menunjukan penggunaan Vesikular Arbuskular Mikoriza meningkatkan pertumbuhan Eleutherine palmifolia (L.) Merr. yang ditanam pada tanah marginal. Presentase kolonisasi VAM tertinggi yaitu 94% dan jumlah spora 48% yang dihasilkan oleh perlakuan M2. Kombinasi M2+NPK efektif untuk meningkatkan pertumbuhan tanaman pada tinggi tanaman (14%), jumlah daun (117%), berat kering tajuk (98%), berat kering akar (15%), berat kering umbi (65%)
Hubungan Kekerabatan Fenetik Jamur Shiitake (Lentinula edodes (Berk.) Pegler) berdasarkan Karakter Morfologi Nuraeni Ekowati; Rina Sri Kasiamdari; Nursamsi Pusposendjojo; C. J. Soegihardjo
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 28, No 2 (2011)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2011.28.2.267

Abstract

Phenetic relationship of shiitake mushroom (Lentinula edodes (Berk.) Pegler) cultivated in Indonesia especially in Java is still undisclosed. In order to increase adaptability and high production most of cultivators in Indonesia make inter-isolates breeding. Consequently  L. edodes isolates grown in Indonesia may have morphological and genetic diversity. Diversity among the isolates of L.edodes can be determined by morphological and molecular assessments. Isolates originating from different locations can show specific morphological characters, and requires more in-depth assessment of the macroscopic and microscopic morphological characters. Four  L.edodes isolates from Malang, Cianjur, Lembang and Yogyakarta were understudy to determine their morphological characters and phenetic relationship. Degree of similarity between the isolates was carried out through numerical taxonomy approach and the data obtained were analyzed using the Numerical taxonomy program and multivariate analysis system, version 2.1. The results showed that there was diversity of morphology of the four isolates and based on the results of data analysis obtained, the degree of similarity between the isolates ranged between 83-95%. Isolates of L. edodes from Malang to Cianjur showed the closest phenetic relationship (95%) and isolates from Malang to Yogyakarta showed the most distant phenetic relationship (83%). Based on the morphological characters obtained from this research revealed the diversity and phenetic relationship among the four isolates of L. edodes.
The inhibition of Fusarium wilt in Chili by Endophytic Fungi isolated from Green Betel (Piper betle L.) Leaf Andang Syaifudin; Rina Sri Kasiamdari
Journal of Natural Sciences and Mathematics Research Vol 8, No 2 (2022): December
Publisher : Faculty of Science and Technology, Universitas Islam Negeri Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/jnsmr.2022.8.2.13795

Abstract

Green Betel (Piper betle L.) leaves are often used as medicine by local people because they contain antibacterial and antifungal substances. These substances can be produced by plant metabolites and are also the results of metabolites produced by endophytic fungi. Endophytic fungus is an organism that is associated with healthy host tissues without causing disease symptoms. Endophytic fungi from betel leaf are used as biocontrol agents against Fusarium oxysporum causes wilt disease that attacks Chili (Capsicum annuum L.), which is one of the main agricultural commodities in Indonesia. Fusarium wilt disease is a disease that significantly reduces crop yields based on crop yields. This research was conducted in a chili farm in Cangkringan, Sleman, Yogyakarta. This study aimed to test the antagonism of endophytic fungi with the dual culture technique. Three types of green betel leaf endophytic fungi were used for dual culture test against Fusarium oxysporum. The observation result showed the highest inhibition ability of 73.37% dual culture method. The inhibitory mechanisms of green betel leaf endophytic fungal isolates include parasitism, antibiosis, and competition. Endophytic fungi of green betel leaf are effective as biocontrol agents of wilt Fusarium chili disease. ©2022 JNSMR UIN Walisongo. All rights reserved.
Characterization and Pathogenicity of Fusarium oxysporum as the Causal Agent of Fusarium Wilt in Chili (Capsicum annuum L.) REJEKI SITI FERNIAH; BUDI SETIADI DARYONO; RINA SRI KASIAMDARI; ACHMADI PRIYATMOJO
Microbiology Indonesia Vol. 8 No. 3 (2014): September 2014
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (258.32 KB) | DOI: 10.5454/mi.8.3.5

Abstract

Fusarium wilt is a serious disease attacking chili plants in Central Java which cause lost of chili productivity. Fusarium wilt is caused by pathogenic fungi Fusarium oxysporum, which is host specific. The objectives of this research were to characterize the pathogenic F. oxysporum as the causal agent of fusarium wilt in chili plants and to observe the virulence of the pathogen. Fungal pathogen was isolated from Tawangmangu as an endemic area of fusarium wilt in Central Java. The fungi was characterized morphologically and identified molecularly by its internal transcribed spacer regions (ITS regions). Pathogenicity test was done to observe the virulence of the pathogen. One pathogenic strain was isolated from Tawangmangu, Karanganyar and was identified  morphologically and molecularly as F. oxysporum.  
Orchid Mycorrhizal Fungi: Identification of Rhizoctonia from West Kalimantan ROSA SURYANTINI; REINE SUCI WULANDARI; RINA SRI KASIAMDARI
Microbiology Indonesia Vol. 9 No. 4 (2015): December 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2256.142 KB) | DOI: 10.5454/mi.9.4.3

Abstract

Orchid is an ornamental plants with high economic value. The excessive exploitation of orchids threatened or even endangered the species, especially those of the epiphytic orchids (Appendiculla sp., Calanthe vestita, and Bulbophyllum beccarii) in West Kalimantan. The discovery of the interaction between orchids and mycorrhizal fungi raises the possibility of ex situ conservation of orchids and it will ensure the success of orchid conservation. Orchid mycorrhizal fungi belongs to the group of Rhizoctonia-like,in which comprised of different genus such as Ephulorhiza, Ceratoriza, and Tullasnela. So far, there is no report on the identit of orchid mycorrhiza associated with the epiphytic orchids in West Kalimantan. The purpose of this study was to identify Rhizoctonia-like associated with Appendiculata sp., Calanthe vestita, and Bulbophyllum beccarii roots in the forest of Raya Pasi and Gunung Bawang, West Kalimantan. The methods were isolation and identification of Rhizoctonia-like from healthy orchid's root based on their morphological characteristics (such as the colony colour, hyphal cell size, sclerotial, concentric circles and monilioid cell, number of nuclei per cell), observation of peloton in root tissue and grouping of isolates. Based on identification of orchid mycorrhiza on the roots of the three species of orchids from West Kalimantan, it was observed that Ceratorhiza sp. was associated with Appendiculla sp.,Ephuloriza sp. with C. vestita, and Tullasnela sp. with B. beccarii roots, respectively. This result is preliminary information and it is still need to be further studied, especially on the role of Rhizoctonia-liker as orchid mycorrhizal fungi in association with the epiphytic orchid for conservation. 
Co-Authors ', Padil AA Sudharmawan, AA Achmadi Priyatmojo Adiana Nayogyani Ali Djamhuri Ali, Nur Ayu Aisyah Ana Susianti Ani Widiastuti, Ani Arliani, Hida Auli, Nisa Raudatul Ayundai, Melin Ayundai, Melin BUDI SETIADI DARYONO BUDI SETIADI DARYONO C. J. Soegihardjo C.J. Soegihardjo C.J. SOEGIHARDJO C.J. Soegihardjo Chasanah, Laila Uswatun Dhia Salsabila Hakim Dian Fitriarni Ekowati , Nuraeni Elzahra Nadya Putri ENDANG SUTARININGSIH SOETARTO Endang Sutariningsih Soetarto Erni Martani Erni Martani Febriani, Anya Via Ganies Riza Aristya Hasnaul Maritsa I Gusti Wayan Murjana Yasa Inayati, Evi Indra Sukmawati Junita Hardini Khaterine K Kristamtini Kristamtini, Kristamtini Kusrinah Kusrinah Kusrinah Kusrinah Laila Hanum Maulin Nafisa Miftahul Ilmi, Miftahul Muazam, Arif Muslikhin Hidayat Nita Aminasih Nuraeni Ekowati Nuraeni Ekowati Nuraeni Ekowati Nuraeni Ekowati, Nuraeni Nursamsi Pusposendjojo Nursamsi Pusposendjojo Nursamsi Pusposendjojo Nursamsi Pusposendjojo, Nursamsi Nursela, Dewi Padil, Padil Purnomo Purnomo Pusposendjojo , Nursamsi Putranto, Dwiyandito Ikhsan Putri, Elzahra Nadya Putri, Fauzana Putri, Fauzana R. Rugayah Ratna Stia Dewi Ratna Stia Dewi Reine Suci Wulandari Rejeki Siti Ferniah Retno Peni Sancayaningsih RETNO PENI SANCAYANINGSIH Retno Peni Sancayaningsih Ristiyani Khofifa Putri ROSA SURYANTINI Rugayah Rugayah S. Daryono, Budi S. Santosa Santosa . Santosa Santosa Santosa Santosa Sari, Noorkomala Setyorini Widyayanti, Setyorini Singgih Tri Wardana Siti Syamsiah Siti Syamsiah Soegihardjo , C.J. Soekarti Moeljopawiro Soekarti Moeljopawiro, Soekarti Suharno Suharno Supriyadi Supriyadi Susiana Purwantisari Sutikno S Syaifudin, Andang Syamsiah, Siti Widiastuti, Ani Yekti Asih Purwestri Yudhistira Nugraha Zakaria Zakaria