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Resistance of Three Maize Varieties to Downy Mildew Fungus Peronosclerospora maydis Infection Ristiyani Khofifa Putri; Rina Sri Kasiamdari
Berkala Ilmiah Biologi Vol 14 No 1 (2023)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v14i1.5658

Abstract

Maize (Zea mays L.) is one of the staple foodstuffs of the cereal group which has a high percentage of carbohydrates, protein, and others. In 2050, maize is expected to double in demand, however, productivity must be balanced with the support management of diseases and pests of maize. Common maize disease is Downy mildew caused by Peronosclerospora spp., this disease attacks the leaves and seeds with 100% damage. This research aimed to determine the pathogenicity and resistance of Talenta, Pioneer 21, and Tamara maize varieties, by using in vivo leaf insertion and leaf spray methods. Fungal isolates were isolated from threshing conidia from maize leaves in the Sleman area field. Observation of fungal pathogenicity and maize resistance was measured based on symptom scale per two days of test and observation of leaf anatomy 45 days after planting. Calculation of fungal pathogenicity against maize varieties used growth analysis, disease incidence, disease severity, Area Under the Disease Progress Curve (AUDPC), and severity unit per time. The result of this research showed that Talenta was moderately resistant, Pioneer-21 was resistant, and Tamara was susceptible to Downy mildew. Downy mildew pathogenicity rate reached 0,07 units/day. Downy mildew infection reached epidermis tissue through stomata on maize leaves.
Identifikasi dan Seleksi Fungi Endofit Daun Sirih Hijau (Piper betle L.) Penghasil Enzim Selulase Dhia Salsabila Hakim; Rina Sri Kasiamdari
Berkala Ilmiah Biologi Vol 14 No 3 (2023)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v14i3.8714

Abstract

Abstract: Endophytic fungi were known in bioethanol industries due to their role in secreting secondary metabolites in the form of cellulolytic enzymes, which were known to be superior to bacteria. The secretion of metabolic enzymes depended on the host they invaded. Herbal plants were recognized as habitats for various endophytic fungi. Betel leaf (Piper betle L.) was one such herbal plant known for its high metabolite content. The aim of this study was to identify endophytic fungi living on betel leaf, conduct cellulolytic tests on the identified fungi, and determine which fungi exhibited the highest cellulolytic activity. The study yielded three species of endophytic fungi from betel leaf: Fusarium graminearum, Fusarium oxysporum, and Colletotrichum coccodes. All three samples showed positive results, producing cellulase enzymes characterized by the presence of a clear zone around the colonies. F. oxysporum was identified as the highest producer of cellulase enzymes, with a concentration of 0,033 U/ml.
Antifungal Effect of Leaf Extracts on the Groundnut Late Leaf Spot Pathogen Cercosporidium personatum Putranto, Dwiyandito Ikhsan; Kasiamdari, Rina Sri
Jurnal Perlindungan Tanaman Indonesia Vol 28, No 1 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.87913

Abstract

Late leaf spot of groundnut is caused by the fungal pathogen of Cercosporidium personatum. Efforts to prevent this disease generally use synthetic fungicides that can cause environmental pollution. This study aims to identify the fungal pathogen of groundnut late leaf spot and study the antifungal effect of leaf extract of some plants for leaf spot intensity suppression. Late leaf spot pathogen was identified based on morphological characteristics of hyphae and conidia. The leaf extracts of butterfly pea (Clitoria ternatea), Mexican sunflower (Tithonia diversifolia), basil (Ocimum gratissimum), and sugar apple (Annona squamosa) at a concentration of 25% against C. personatum were evaluated in vitro based on growth inhibition. The growth and intensity of groundnut disease attacks were assessed for seven weeks by inoculating C. personatum and application of the leaf extracts. Results showed that the pathogen was identified as C. personatum with conidia characteristics of cylindrical to obclavate, short and slightly curved with four septa, and 35.75 × 7.11 µm in size. The percentage of inhibition of C. personatum in vitro reached 94.03% by leaf extract of C. ternatea, 88.56% by T. diversifolia, 87.20% by O. gratissimum, and 84.10% by A. squamosa. Treatment of C. ternatea increased plant height up to 12.85% and reduced the most optimal percentage of the infected leaves up to 61.53%. C. ternatea leaf extract was found to be the most effective extract to suppress C. personatum infection by 77.94%, while T. diversifolia, O. gratissimum, and A. squamosa reduced disease severity by 70.15%, 65.43%, and 57.76%, respectively.
Characterization of Peronosclerospora maydis and Its Effect on Plant Growth and Disease Incidence Under Fungicides Treatment on Maize (Zea mays L.) Elzahra Nadya Putri; Rina Sri Kasiamdari
Al-Kauniyah: Jurnal Biologi Vol 18, No 1 (2025): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v1i1.32399

Abstract

AbstractDowny mildew disease on maize (Zea mays L.) is generally caused by fungi from the genus Peronosclerospora. This study aims to identify the type of fungi that cause downy mildew in Sleman, to determine the effect of a fungicide on the growth of maize plants, the disease incidence, and severity. Fungi isolates were taken from the maize plantation area in Tirtoadi Village, Sleman. Observation of fungal spores was carried out using a binocular microscope. Application of metamorph (PMD), metalaxyl (PMMe), propineb (PMP), and mancozeb (PMMz) fungicides was carried out when the plants were 7 days after planting (DAP). Maize plants were inoculated with downy mildew spores when the plants were 8 DAP. The parameters measured were plant height, number of leaves, fresh weight, dry weight, disease incidence, and severity. Data were analyzed by ANOVA using SPSS version 23 and tested by Duncan Multiple Range Test (DMRT). The results showed that the fungi causing downy mildew in Sleman was Peronosclerospora maydis. Plants treated with mancozeb (PMMz) had the highest growth in plant height, number of leaves, fresh weight, and dry weight, followed by PMP, PMD, and PMMe treatments. The results of the DMRT test showed that the disease incidence and disease severity of PMD, PMMe, and PMP were significantly reduced compared to the P. maydis (PM) treatment. Plants with metalaxyl treatment had the highest ability to reduce disease severity by 92.70%, followed by prominent at 78.10%, metamorph 58.66%, and mancozeb 16.79%.AbstrakPenyakit bulai pada tanaman jagung (Zea mays L.) umumnya disebabkan oleh jamur dari genus Peronosclerospora. Penelitian ini bertujuan untuk mengidentifikasi jenis jamur penyebab penyakit bulai di Sleman, mengetahui pengaruh fungisida terhadap pertumbuhan tanaman jagung, kejadian, dan keparahan penyakit. Isolat jamur diambil dari area persawahan Desa Tirtoadi, Sleman. Pengamatan spora jamur dilakukan dengan menggunakan mikroskop binokuler. Pemberian fungisida dimetomorf (PMD), metalaksil (PMMe), propineb (PMP), dan mankozeb (PMMz) dilakukan saat tanaman berumur 7 hari setelah tanam (HST). Tanaman jagung diinokulasi dengan spora bulai pada saat tanaman berumur 8 HST. Parameter yang diukur adalah tinggi tanaman, jumlah daun, berat basah, berat kering, kejadian penyakit, dan tingkat keparahan. Data dianalisis dengan ANOVA menggunakan SPSS versi 23 dan diuji dengan Duncan Multiple Range Test (DMRT). Hasil penelitian menunjukkan bahwa jamur penyebab bulai di daerah Sleman adalah P. maydis. Tanaman dengan perlakuan PMMz memiliki pertumbuhan tinggi tanaman, jumlah daun, berat basah, dan berat kering tertinggi, diikuti dengan perlakuan PMP, PMD, dan PMMe. Hasil uji DMRT menunjukkan angka kejadian penyakit dan keparahan penyakit pada perlakuan PMD, PMMe, dan PMP menurun secara signifikan dibandingkan dengan perlakuan P. maydis (PM). Tanaman dengan perlakuan metalaksil memiliki kemampuan terbaik dalam menurunkan keparahan penyakit sebesar 92,70%, kemudian propineb 78,10%, dimetomorf 58,66%, dan mankozeb 16,79%. 
Binucleate Rhizoctonia SEBAGAI PENGHAMBAT Peronosclerospora maydis PENYEBAB PENYAKIT BULAI PADA TANAMAN JAGUNG (Zea mays L.) Ali, Nur Ayu Aisyah; Kasiamdari, Rina Sri
Jurnal Agrotek Tropika Vol 12, No 2 (2024): JURNAL AGROTEK TROPIKA VOL 12, Mei 2024
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v12i2.8106

Abstract

Salah satu komoditas pertanian terbesar di Indonesia adalah jagung (Zea mays L.). Produksi jagung yang dihasilkan cukup signifikan tetapi dalam proses penanaman terdapat kendala. Salah satunya akibat serangan penyakit bulai yang disebabkan oleh jamur Peronosclespora maydis. Tujuan penelitian ini adalah untuk mengetahui potensi BNR dalam menghambat jamur penyebab penyakit bulai secara in vivo. Penelitian ini dilakukan dalam Rancangan Acak Kelompok (RAK) dengan empat perlakuan dan empat ulangan. Perlakuan terdiri dari Kontrol (P0), BNR (P1), P. maydis (P2) dan BNR + P. maydis (P3) serta penggunaan varietas Pertiwi dan Pioneer-21. Hasil penelitian menunjukan bahwa jamur endofit yang digunakan sebagai penghambat jamur penyebab penyakit bulai adalah Binucleate Rhizoctonia dan jamur penyebab bulai yang ditemukan adalah Peronosclerospora maydis. BNR berpotensi menghambat pertumbuhan jamur P. maydis secara in vivo pada varietas Pertiwi sebesar 72% dan Pioneer-21 sebesar 67% serta dapat meningkatkan pertumbuhan tanaman jagung.
Kolonisasi mikoriza Claroideoglomus etunicatum dalam menurunkan penyakit busuk akar Rhizoctonia solani pada kacang hijau Chasanah, Laila Uswatun; Kasiamdari, Rina Sri
Jurnal AGRO Vol 11, No 2 (2024)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/36464

Abstract

Mung bean (Vigna radiata (L.) R. Wilczek) is widely cultivated in Indonesia, but root rot disease caused by Rhizoctonia solani can decrease production. Arbuscular mycorrhiza can be an alternative biocontrol to control root rot. This research aimed to determine the character of R. solani, analyze the growth of mung bean infected by R. solani inoculated with mycorrhizal fungi Claroideoglomus etunicatum, and determine the severity suppression of root rot disease in mung bean inoculated with mycorrhizal fungi C. etunicatum. The study used a Completely Randomized Design (CRD) with six treatments and four replications including control (without inoculation), R. solani, 120 spores of C. etunicatum, 180 spores of C. etunicatum, 120 spores of C. etunicatum + R. solani, and 180 spores of C. etunicatum + R. solani. This research showed that R. solani had white to brown colonies, cottony, septate hyphae with 90° branching, hyphae width 5.45 – 9.79 μm, multinucleate, slow growth with an abundant-aerial pattern. Inoculation of C. etunicatum on mung bean infected by R. solani had a significant effect on increasing shoot fresh weight, shoot dry weight, and pod length and C. etunicatum reduced the intensity of R. solani disease by 44.44 – 55.46% and reduced the infection of R. solani in mung bean root by 38.10 – 52.38%. In conclusion, C. etunicatum was able to suppress the severity of rot root disease caused by R. solani on mung bean. ABSTRAK Kacang hijau (Vigna radiata (L.) R. Wilczek) banyak dibudidayakan di Indonesia, tetapi penyakit busuk akar akibat Rhizoctonia solani menyebabkan penurunan jumlah produksi. Mikoriza arbuskular dapat menjadi alternatif biokontrol untuk mengendalikan busuk akar. Penelitian ini bertujuan untuk mengetahui karakter jamur R. solani, menganalisis pertumbuhan kacang hijau terinfeksi R. solani yang diinokulasi jamur mikoriza Claroideoglomus etunicatum, serta mengetahui penekanan keparahan penyakit busuk akar pada kacang hijau yang diinokulasi jamur mikoriza C. etunicatum. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan enam perlakuan dan empat ulangan, yaitu kontrol (tanpa inokulasi), R. solani, 120 spora C. etunicatum, 180 spora C. etunicatum, 120 spora C. etunicatum + R. solani, dan 180 spora C. etunicatum + R. solani. Penelitian ini menunjukkan bahwa R. solani memiliki koloni putih hingga coklat, cottony, hifa bersekat dengan percabangan 90°, lebar hifa 5,45 – 9,79 μm, multinukleat, pertumbuhan slow dengan pola abundant-aerial. Inokulasi C. etunicatum pada kacang hijau terinfeksi R. solani berpengaruh nyata terhadap peningkatan berat basah tajuk, berat kering tajuk, dan panjang polong serta C. etunicatum menurunkan intensitas penyakit R.solani sebesar 44,44% – 55,56% dan menurunkan infeksi R. solani pada akar kacang hijau sebesar 38,10% – 52,38%. Kesimpulan penelitian ini, C. etunicatum mampu menekan keparahan penyakit busuk akar oleh R. solani pada kacang hijau.
DNA Barcoding Using the Chloroplast Gene MatK for Genetic Diversity Analysis of Lansium domesticum Corr. Plants Growing in South Sumatra, Indonesia Wardana, Singgih Tri; Hanum, Laila; Nursela, Dewi; Aminasih, Nita; Kasiamdari, Rina Sri
Biosfer: Jurnal Tadris Biologi Vol 16 No 1 (2025): Biosfer: Jurnal Tadris Biologi
Publisher : UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/biosfer.v16i1.27296

Abstract

Lansium domesticum Corr. known as duku, langsat, and kokosan is a popular fruit in Indonesia, particularly in South Sumatra. Furthermore, the fruit is cherished in this region because of the nice smell, sweet taste, and high economic value. Until now, South Sumatra local duku is rarely investigated, even though information on the genetic diversity of the germplasm is useful in supporting breeding and conservation programs. Therefore, this study aimed to determine the genetic diversity and phylogenetics of local duku and langsat (Lansium domesticum) based on matK gene DNA barcoding. DNA genome was extracted using Tiangen Plant Genomic DNA Kit DP305, then amplified using GoTaq Green Master Mix (Promega) and primers 3F_KIM-F and 1R_KIM-R. The product was sequenced by 1st Base Singapore and seven amplification results had a DNA size of 900 bp. The results showed that the matK gene is capable of determining Lansium domesticum interspecies. According to the DNA sequence phylogenetic analysis conducted using the maximum likelihood method, all the DNA samples were designated as Lansium domesticum Corr. with a fairly high genetic variation, indicated by DNA sequence differences caused by gene mutations. DNA Barcoding Menggunakan Gen Kloroplas MatK untuk Analisis Keragaman Genetik Tanaman Lansium domesticum Corr. yang Tumbuh di Sumatera Selatan, Indonesia ABSTRAK: Lansium domesticum Corr. yang dikenal dengan sebutan duku, langsat, dan kokosan merupakan buah yang populer di Indonesia, khususnya di Sumatera Selatan. Selain itu, buah ini digemari di daerah ini karena baunya yang harum, rasanya yang manis, dan bernilai ekonomis tinggi. Hingga saat ini, duku lokal Sumatera Selatan jarang diteliti, padahal informasi mengenai keragaman genetik plasma nutfahnya bermanfaat dalam mendukung program pemuliaan dan konservasi. Oleh karena itu, penelitian ini bertujuan untuk mengetahui keragaman genetic dan filogenetik duku dan langsat (Lansium domesticum) berdasarkan DNA barcoding gen matK. DNA genom diekstraksi menggunakan Tiangen Plant Genomic DNA Kit DP305, kemudian diamplifikasi menggunakan GoTaq Green Master Mix (Promega) dan primer 3F_KIM-F dan 1R_KIM-R. Produk tersebut disekuensing oleh 1st Base Singapore dan tujuh hasil amplifikasi memiliki ukuran DNA 900 bp. Hasil penelitian menunjukkan bahwa gen matK mampu mengidentifikasi Lansium domesticum antarspesies. Berdasarkan analisis filogenetik sekuens DNA yang dilakukan dengan metode kemungkinan maksimum, semua sampel DNA ditetapkan sebagai Lansium domesticum Corr. dengan variasi genetik yang cukup tinggi, ditunjukkan oleh perbedaan sekuens DNA yang disebabkan oleh mutasi gen.
PROXIMATE, TANNIN, AND AMYLOSE IN SIX SORGHUM VARIETIES ACROSS TWO PLANTING SEASONS Muazam, Arif; Kristamtini, Kristamtini; Widyayanti, Setyorini; Nugraha, Yudhistira; Kasiamdari, Rina Sri; Setiadi Daryono, Budi
BIOTROPIA Vol. 32 No. 2 (2025): BIOTROPIA Vol. 32 No. 2 August 2025
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2025.32.2.2461

Abstract

ARTICLE HIGLIGHTS- Sorghum nutrition varies across varieties and growing seasons- Dry season increases starch and amylose content in some varieties- Rainy season enhances protein levels in sorghum grains- Genotype-environment interaction affects sorghum nutritional quality- Sorghum adaptation supports food diversification and climate resilienceABSTRACTSorghum (Sorghum bicolor (L.) Moench) is a potential staple food in Indonesia, offering an alternative to rice and corn due to its adaptability to marginal lands and drought tolerance. However, research on the impact of environmental factors, particularly different growing seasons, to the nutritional composition of sorghum varieties are still lacking. This study aimed to analyze the proximate contents of tannin and amylose in six sorghum varieties cultivated in two different planting seasons (wet and dry seasons). A Completely Randomized Design with three replications was applied in this experiment. The tested varieties included three newly developed superior varieties (Bioguma, Samurai, and Kawali) and three local varieties (Black Bonteb, Red Glutinous Sorghum, and KD4). The results showed that proximate composition varied across varieties and seasons. Starch content was generally higher in the dry season, with the highest observed in Kawali (64.16%) and Bioguma (63.11%), while the lowest was in Black Wareng (11.99%). Similarly, protein content peaked in Black Wareng (7.68%) and Red Glutinous Sorghum (7.37%), but was the lowest in Samurai (4.48%). Tannin levels also fluctuated by season, where Bioguma, KD4, and Kawali exhibited higher tannin content in the dry season, whereas Samurai, Red Glutinous Sorghum, and Black Bonteb had lower tannin levels. Notably, amylose content was significantly higher in Red Glutinous Sorghum (4.72%) and Black Bonteb (4.62%) across both seasons, contributing to their fluffier texture when cooked. These findings highlight the influence of environmental conditions on sorghum nutritional quality, reinforcing its potential for food diversification, including processed sorghum flour as a substitute for wheat flour.
Identification of Curvularia eragrostidis (Henn.) J.A.Mey. the Leaf Spot Pathogen of Oil Palm (Elaeis guineensis Jacq.) and It’s Control by False Elder (Peronema canescens Jack) Leaf Extract Febriani, Anya Via; Kasiamdari, Rina Sri
International Journal of Oil Palm Vol. 6 No. 2 (2023)
Publisher : Indonesian Oil Palm Society /IOPS (Masyarakat Perkelapa-sawitan Indonesia /MAKSI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35876/ijop.v6i2.111

Abstract

Oil palm is the main vegetable oil-producing crop in Indonesia. Leaf spot disease is one of the major diseases that attacks oil palm seedlings at all seedling stages. In Indonesia, leaf spot disease in oil palm nurseries is most commonly caused by the genus Curvularia with an infection intensity of up to 60–70%. The control of leaf spot disease usually uses chemical fungicides but its continuous use can cause the development of resistant pathogen fungi and have a long-term negative impact on the environment. False elder (Peronema canescens Jack) leaves have bioactivity as an antimicrobial control disease caused by fungal infections. In this study, Curvularia was isolated from oil palm seedlings infected with leaf spot disease from oil palm nurseries in South Sumatra. Effectiveness testing of P. canescens leaves conducted by a Completely Randomized Design method with five treatments and three replications. The fungi that caused leaf spot disease in oil palm were identified as Curvularia eragrostidis. P. canescens leaf water extract at 25% concentration was very ineffective to quite effective in controlling disease severity based on the average number of spots and diameter of spots in oil palm with the values 36.25–59.50% and 12.50–27.78%. P. canescens leaf water extract could reduce the average number of spots and diameter of spots started on day 30 after being sprayed three times with P. canescens leaf water extract so that it could be used as an alternative to control C. eragrostidis leaf spot disease in oil palm that is more friendly to the environment.
Characterization of Peronosclerospora maydis and Its Effect on Plant Growth and Disease Incidence Under Fungicides Treatment on Maize (Zea mays L.) Putri, Elzahra Nadya; Kasiamdari, Rina Sri
Al-Kauniyah: Jurnal Biologi Vol. 18 No. 1 (2025): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v1i1.32399

Abstract

AbstractDowny mildew disease on maize (Zea mays L.) is generally caused by fungi from the genus Peronosclerospora. This study aims to identify the type of fungi that cause downy mildew in Sleman, to determine the effect of a fungicide on the growth of maize plants, the disease incidence, and severity. Fungi isolates were taken from the maize plantation area in Tirtoadi Village, Sleman. Observation of fungal spores was carried out using a binocular microscope. Application of metamorph (PMD), metalaxyl (PMMe), propineb (PMP), and mancozeb (PMMz) fungicides was carried out when the plants were 7 days after planting (DAP). Maize plants were inoculated with downy mildew spores when the plants were 8 DAP. The parameters measured were plant height, number of leaves, fresh weight, dry weight, disease incidence, and severity. Data were analyzed by ANOVA using SPSS version 23 and tested by Duncan Multiple Range Test (DMRT). The results showed that the fungi causing downy mildew in Sleman was Peronosclerospora maydis. Plants treated with mancozeb (PMMz) had the highest growth in plant height, number of leaves, fresh weight, and dry weight, followed by PMP, PMD, and PMMe treatments. The results of the DMRT test showed that the disease incidence and disease severity of PMD, PMMe, and PMP were significantly reduced compared to the P. maydis (PM) treatment. Plants with metalaxyl treatment had the highest ability to reduce disease severity by 92.70%, followed by prominent at 78.10%, metamorph 58.66%, and mancozeb 16.79%.AbstrakPenyakit bulai pada tanaman jagung (Zea mays L.) umumnya disebabkan oleh jamur dari genus Peronosclerospora. Penelitian ini bertujuan untuk mengidentifikasi jenis jamur penyebab penyakit bulai di Sleman, mengetahui pengaruh fungisida terhadap pertumbuhan tanaman jagung, kejadian, dan keparahan penyakit. Isolat jamur diambil dari area persawahan Desa Tirtoadi, Sleman. Pengamatan spora jamur dilakukan dengan menggunakan mikroskop binokuler. Pemberian fungisida dimetomorf (PMD), metalaksil (PMMe), propineb (PMP), dan mankozeb (PMMz) dilakukan saat tanaman berumur 7 hari setelah tanam (HST). Tanaman jagung diinokulasi dengan spora bulai pada saat tanaman berumur 8 HST. Parameter yang diukur adalah tinggi tanaman, jumlah daun, berat basah, berat kering, kejadian penyakit, dan tingkat keparahan. Data dianalisis dengan ANOVA menggunakan SPSS versi 23 dan diuji dengan Duncan Multiple Range Test (DMRT). Hasil penelitian menunjukkan bahwa jamur penyebab bulai di daerah Sleman adalah P. maydis. Tanaman dengan perlakuan PMMz memiliki pertumbuhan tinggi tanaman, jumlah daun, berat basah, dan berat kering tertinggi, diikuti dengan perlakuan PMP, PMD, dan PMMe. Hasil uji DMRT menunjukkan angka kejadian penyakit dan keparahan penyakit pada perlakuan PMD, PMMe, dan PMP menurun secara signifikan dibandingkan dengan perlakuan P. maydis (PM). Tanaman dengan perlakuan metalaksil memiliki kemampuan terbaik dalam menurunkan keparahan penyakit sebesar 92,70%, kemudian propineb 78,10%, dimetomorf 58,66%, dan mankozeb 16,79%. 
Co-Authors ', Padil AA Sudharmawan, AA Achmadi Priyatmojo Adiana Nayogyani Ali Djamhuri Ali, Nur Ayu Aisyah Ana Susianti Ani Widiastuti, Ani Arliani, Hida Auli, Nisa Raudatul Ayundai, Melin Ayundai, Melin BUDI SETIADI DARYONO BUDI SETIADI DARYONO C. J. Soegihardjo C.J. Soegihardjo C.J. SOEGIHARDJO C.J. Soegihardjo Chasanah, Laila Uswatun Dhia Salsabila Hakim Dian Fitriarni Ekowati , Nuraeni Elzahra Nadya Putri Endang Sutariningsih Soetarto ENDANG SUTARININGSIH SOETARTO Erni Martani Erni Martani Febriani, Anya Via Ganies Riza Aristya Hasnaul Maritsa I Gusti Wayan Murjana Yasa Inayati, Evi Indra Sukmawati Junita Hardini Khaterine K Kristamtini Kristamtini, Kristamtini Kusrinah Kusrinah Kusrinah Kusrinah Laila Hanum Maulin Nafisa Miftahul Ilmi, Miftahul Muazam, Arif Muslikhin Hidayat Nita Aminasih Nuraeni Ekowati Nuraeni Ekowati Nuraeni Ekowati Nuraeni Ekowati, Nuraeni Nursamsi Pusposendjojo Nursamsi Pusposendjojo Nursamsi Pusposendjojo Nursamsi Pusposendjojo, Nursamsi Nursela, Dewi Padil, Padil Purnomo Purnomo Pusposendjojo , Nursamsi Putranto, Dwiyandito Ikhsan Putri, Elzahra Nadya Putri, Fauzana Putri, Fauzana R. Rugayah Ratna Stia Dewi Ratna Stia Dewi Reine Suci Wulandari Rejeki Siti Ferniah Retno Peni Sancayaningsih Retno Peni Sancayaningsih RETNO PENI SANCAYANINGSIH Ristiyani Khofifa Putri ROSA SURYANTINI Rugayah Rugayah S. Daryono, Budi S. Santosa Santosa . Santosa Santosa Santosa Santosa Sari, Noorkomala Setyorini Widyayanti, Setyorini Singgih Tri Wardana Siti Syamsiah Siti Syamsiah Soegihardjo , C.J. Soekarti Moeljopawiro Soekarti Moeljopawiro, Soekarti Suharno Suharno Supriyadi Supriyadi Susiana Purwantisari Sutikno S Syaifudin, Andang Syamsiah, Siti Widiastuti, Ani Yekti Asih Purwestri Yudhistira Nugraha Zakaria Zakaria