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All Journal International Journal of Evaluation and Research in Education (IJERE) Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Indonesian Journal of Islam and Muslim Societies Indonesian Journal of Urology Jurnal Bioterdidik: Wahana Ekspresi Ilmiah Bioedukasi: Jurnal Pendidikan Biologi Jurnal Pendidikan Biologi Indonesia Biology, Medicine, & Natural Product Chemistry Jurnal Florea UNEJ e-Proceeding BIOEDUKASI Diklabio: Jurnal Pendidikan dan Pembelajaran Biologi Berkala Penelitian Hayati Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Ta'dib Jurnal Ilmu Hayat

Umie Lestari

Universitas Negeri Malang

Author-ID : 407885

Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Other

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Profile protein autism analysis based bioinformatics as public dissemination materials in Malang about the potensial causes autism Rizki Mei Listawati; Umie Lestari; Mohammad Amin
JPBI (Jurnal Pendidikan Biologi Indonesia) Vol. 2 No. 3 (2016): NOVEMBER
Publisher : University of Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/jpbi.v2i3.3876

Kehidupan saat ini menawarkan berbagai kemudahan. Namun, seiring dengan hal tersebut ada banyak permasalahan yang memicu stress. Selain itu, terdapat banyak unsur kimia dan logam berat yang jika saling berinteraksi dapat menimbulkan berbagai macam penyakit mulai dari flu hingga kanker, termasuk autis. Penelitian ini bertujuan untuk menganalisis potensi profil protein dalam darah yang menyebabkan autis berdasarkan bioinformatik. Kemudian, disosialisasikan kepada masyarakat khususnya orang tua anak autis di Kota Malang sehingga dapat memberikan wawasan tentang autis. Penelitian ini juga dapat dikembangkan sebagai dasar penelitian laboratorium. Jenis penelitian ini adalah deskriptif kualitatif, menggunakan tiga database yaitu National Center for Biotechnology Information (NCBI), Uniprot, dan Protein Data Bank (PDB) serta menggunakan software Pymol. Hasil penelitian ini menunjukkan bahwa: 1) Protein dalam darah yang berpotensi menyebabkan autis berdasarkan artikel yaitu mTOR, Glutathione S Transferase P, Chemokine Monocyte Chemotactic Protein-1 (MCP-1), Interferon-∂-Inducible Protein-16 (IFI-16), dan serotonin; 2) Protein-protein tersebut setelah dianalisis dengan menggunakan tiga database (NCBI, Uniprot, dan PDB) menunjukkan bahwa protein Glutathione S Transferase P lebih berpotensi terhadap autis. Hal ini karena protein tersebut berfungsi untuk mencegah neurodegeneration; 3) Penelitian tentang penyebab autis sangat dibutuhkan oleh orang tua anak autis (masyarakat), sehingga dapat diminimalisir akses masuknya pemicu tersebut.

Quercetin: the bioactive compound from Allium cepa L. as anti-inflammation based on in silico screening Mohamad Amin; Kurniawan Setia Putra; Ihya Fakhrurizal Amin; Nanda Earlia; Dina Maulina; Betty Lukiati; Umie Lestari
Biology, Medicine, & Natural Product Chemistry Vol 7, No 1 (2018)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Inflammation is a tissue injury that occurs due to physical trauma or microbiological substances that involve the activities of many cell types. Inflammation can be prevented using the natural medicines from Allium cepa L. Quercetin is one of the bioactive compounds found in Allium cepa L and has been reported to have anti-inflammatory activity. The natural medicines have been used to minimize non-steroidal anti-inflammatory drugs. This study aims to investigated the modeling structures and the protein receptor from quecertin in inflammation mechanism and their optimization of the effectiveness in the human body. The bioinformatics tools used in this study are the database of quercetin compounds, Pubchem and Swis Target Prediction protein prediction databases, PyRx 0.8 molecular docking software, ligand docking, and binding site analysis with PyMOL and LigPlus software. The results from in silico show that quercetin compounds can interact with Muscleblind-like protein 1 target protein with a Binding Affinity minus value which is not much different from the dexamethasone compound. Dexamethason is a standart because it is a corticosteroid drug that can be used as an anti-inflammatory to reduce inflammation, allergic reactions, arthritis and other inflammatory diseases.

Potensi Genistein Terhadap Histopatologi Tubulus Seminiferus Testis Mencit (Mus musculus) Cicilia Novi Primiani; Umie Lestari
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 17, No 2 (2012): June 2012
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v17i2.130

Pemanfaatan sarana kontrasepsi pria dengan memanfaatkan bahan alami berasal dari tanaman sudah banyak dilakukan. Tetapi pemanfaatan biji kedelai sebagai salah satu tanaman suku Leguminoceae belum pernah dilakukan. Biji kedelai mengandung senyawa genistein, sebagai salah satu senyawa derivat isoflavon mempuyai struktur kimia mirip dengan 17Î²-estradiol bersifat seperti hormon steroid estrogen, mampu menyebabkan kerusakan pada sistem reproduksi jantan. Penelitian bertujuan menguji pengaruh genistein terhadap histopatologi tubulus seminiferus testis mencit jantan (Mus musculus). Penelitian dilakukan menggunakan pendekatan eksperimental, yang rancangannya mengikuti rancangan acak lengkap. Variabel bebas adalah dosis genistein 0 mg/g, 0,0035 mg/g, 0,0042 mg/g, dan 0,0049 mg/g. Variabel terikat adalah sel-sel germinal tubulus seminiferus testis. Data rerata jumlah sel-sel germinal dianalisis menggunakan Analisis Varians Satu Arah (One Way ANOVA) dengan tingkat signifikansi 95% (Î± 5%) dilakukan uji Post Hoc dengan Least Significant Difference (LSD) Î± 5%. Perubahan pada jaringan testis dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa ada pengaruh pemberian genistein terhadap rerata jumlah sel-sel germinal dan menyebabkan penghambatan proliferasi sel-sel germinal dalam tubulus seminiferus testis.

PENGEMBANGAN BAHAN AJAR BERBASIS PENELITIAN KARAKTERISASI PROTEIN MEMBRAN SPERMA PADA MATAKULIAH BIOTEKNOLOGI Ika Oktaviana; Sutiman B Sumitro; Umie Lestari
Florea : Jurnal Biologi dan Pembelajarannya Vol 2, No 2 (2015)
Publisher : UNIVERSITAS PGRI MADIUN

The purpose of the research and development is to produce teaching materials such as textbooks supporting course in Biotechnology research based on the characterization of the sperm membrane proteins for students who take a course in Biotechnology. This research is a research and development, where the results of research become the teaching materials developed supporting Biotechnology course. Development models have prepared using ADDIE Model. Sources of data in this research include laboratory research and validation of the results sheet of Biotechnology material experts, instructional design experts, the media, and the trials in students. The analysis is used descriptive analysis and quantitative descriptive exploratory. The results of the research have developed become teaching materials like text books supporting courses and have been validated by the Biotechnology, the biotechnology material experts, instructional design experts, the media, the individual testing, and group trials. Based on the results of the validation material have obtained value of 89.56%, the results of the design validation and media experts to get the value of 93.75%, it means that the teaching materials has been developed which suitable to be used as a teaching material support biotechnology course. While the experiments performed to get a data the percentage of students that are equal to 88.98% and included in the category of “very good”, but still need to be revised based on advices and comments from students.

PENGEMBANGAN BAHAN AJAR MATA KULIAH FISIOLOGI REPRODUKSI DI JURUSAN BIOLOGI UNIVERSITAS NEGERI MALANG Farizha Irmawati; Umie Lestari; Hadi Suwono
Florea : Jurnal Biologi dan Pembelajarannya Vol 2, No 2 (2015)
Publisher : UNIVERSITAS PGRI MADIUN

The purpose of the research and development is to produce teaching materials such as textbooks supporting course in Reproduction Physiology research based on the toxicity analysis for students who take a course in Reproduction Physiology. This research is a research and development, where the results of research become the teaching materials developed supporting Reproduction Physiology course. Development models have prepared using ADDIE Model. Sources of data in this research include laboratory research and validation of the results sheet of Biotechnology material experts, instructional design experts, the media, and the trials in students. The analysis is used quantitative and qualitative.The results of the research have developed become teaching materials like text books supporting courses and have been validated by the Reproduction Physiology, the Reproduction Physiology material experts, instructional design experts, the media, the individual testing, and group trials. Based on the results of the validation material have obtained value of 95%, the results of the design validation and media experts to get the value of 93.75%, it means that the teaching materials has been developed which suitable to be used as a teaching material support biotechnology course. While the experiments performed to get a data the percentage of students that are equal to 88,25 % and included in the category of “very good”, but still need to be revised based on advices and comments from students.

THE EFFECTS OF WATER FRACTION OF BITTER MELON (MOMORDICA CHARANTIA) LEAF EXTRACT IN MAMMARY GLAND DEVELOPMENT OF BALB/C MICE (MUS MUSCULUS) WITH HISTOLOGICAL AND MOLECULAR BIOLOGICAL ANALYSIS OF PROTEIN APPROACHES Nur Hayati; Afifah Nur Aini; Nafisatuzzamrudah Nafisatuzzamrudah; Umie Lestari
UNEJ e-Proceeding 2016: Proceeding The 1st International Basic Science Conference
Publisher : UPT Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Recently many breast-feeding mothers use chemical drugs to ease milk secretion, while the said drug could cause side effects if consumed in a long time. The problem could be solved by using natural medicine like bitter melon leaf because it contains charantin which is characteristically identical to phytosterol [6]. This study aimed to discover the potention of water fraction of bitter melon leaf extract to stimulate mammary gland development in Balb/c mice. The development of mammary gland was observed through histological approach by calculating the number of cells in mammary gland, and through molecular biological analysis of protein approach by using SDS-PAGE. The histology of mammary gland showed that the administration of the extract with concentration of 11,200 ppm had the highest number of cells, followed by 5,600 ppm; 22,400 ppm and 16,800 ppm. The result of SDS-PAGE on isolated mammary gland protein showed that the molecular weight of the proteins in the controlled treatment were 2, 5, 8, 10, 12, 19, 34, 40 and 49 kDa. In treatment with concentration of 5,600 ppm there were only 10, 12, 34, 40 and 49 kDa. In treatment with concentration of 11,200 ppm there were similar kinds of proteins with the controlled treatment, only without the 8 kDa protein. In treatment with concentration of 16,800 and 22,400 ppm, there were only 2, 5, 10, 12 kDa. The conclusion of this study was water fraction of bitter melon leaf extract could affect the development of mammary gland in Balb/c mice.

MORPHOMETRIC CHARACTERS OF MARINE LOCAL FISH (Harpodon sp.) FROM TARAKAN, NORTHERN BORNEO Endik Deni Nugroho; Dwi Anggorowati Rahayu; Moh. Amin; Umie Lestari
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 21 No 1 (2015): December 2015
Publisher : The East Java Biological Society

Tarakan sea waters have geographical circumstances and landscapes that supported the existence richness of fishes that abundant and diverse. There is local fish called Pepija or Lembe-Lembe, but biological studies on this fish most poorly known. This research is aimed to identify the morphological characters of Nomei (Harpodon sp.). Based on specific caharcter, morphometric, and meristric characters. Morphometrics analysis can be used to clustering and understanding relationship of taxa. Each species represented 10% samples of male and female from each type we found and measured 35 characters (in mm). The canonical, clustering and similarity were used. Based on determinant morphological characters species (pectoral fin length reaches or exceeds the initial dorsal fin, dorsal fin earlier than ventral fins), dendogram morphological characters, meristic characters, PCA grouping proved that Nomei fish from Amal and Juata as Harpo-don nehereus.

THE DYNAMICS OF 1.3-B-D-GLUCAN GIVEN IN THE FORM OF AGARICUS BLAZEI MURILL POWDER TO THE BODY OF WHITE MICE Sprague Dawley AND EFFECT OF RENAL AND HEPATIC HISTOPATHOLOGY Misgiati Misgiati; Umie Lestari; Aloysius Duran Corebima
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 19 No 1 (2013): December 2013
Publisher : The East Java Biological Society

Dynamics of compound 1,3-Î²-glucan in Agaricus Blazei Murill (ABM) powder and the effect of renal and hepatic histopathology performed on 24 male rats aged 2 months, were randomly conducted in three treatments there are control, ABM powder, and the pure compound 1.3-Î²-glucan for 28 days. Provision of treatment given in the morning and then 1, 3, 8, 12, and 24 hours after administration of treatment blood was collected, while for urine and feces were collected every 8 hours. Giving treatment was continued until day 28. The results showed that the levels of 1,3-Î²-glucan in pure compounds is higher than the Agaricus Blazei Murill (ABM) powder. The effects on the kidneys and liver histopathology showed that cells necrosis more on pure compounds from the mushroom powder gods, both the kidneys and liver. This study shows that the use of pure compounds to be more careful than Agaricus Blazei Murill (ABM) powder in determining the doses used.

THE DYNAMICS OF DAIDZEIN CHEMICAL COMPOUND FROM YAM TUBER (Pachyrhizus erosus) TUBER AND ITS POTENTIAL IN MYOMETRIUM Cicilia Novi Primiani; Umie Lestari; Mohammad Amin; Sutiman B. Sumitro
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 18 No 2 (2013): June 2013
Publisher : The East Java Biological Society

Daidzein compounds dynamic and the effect of yam tuber (Pachyrhizus erosus (L)) Urb on myometrium rats model of estrogenic were investigated. Twentyfour of 5 months-old female Sprague Dawley rats were randomly assigned to three groups as followed, control, Daidzein administraton and yam tuber juice administration for 24 days. The administration of yam tuber juice were given by orally using a stomach with gavage tube in the morning. Every 8 h, the rats blood and urine were collected from tail until 24 h. The treatment were continued until 24 days. The result showed that the level of daidzein in blood and urine of rats that administratered by Yam tuber juice is lower than pure daidzein treatment rats every 8 until 24 hours of collection. The results demonstrated that the administration of yam tuber juice significantly myometrium proliferated in rats. It is assumed that genistein and quercetin is estrogenic as found in daidzein so that the miometrium is potentially more effecctive. This study suggest that phytoestrogen compound from Pachyrhizus erosus may offer a potential as natural estrogenic in women.

Nutritional profile of kian marine worm from kei islands maluku as a potential high protein food resource Siti Rahma Lestaluhu; Siti Nur Arifah; Umie Lestari; Sri Rahayu Lestari
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 26 No 1 (2020): December 2020
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/bphjbr.26.1.20203

The nutritional content of kian marine worm has never been reported. This study aimed to profile the nutritional content of worms from the Kei Islands, Maluku. Nutritional profiles were tested by proximate assays, atomic absorption spectroscopy (AAS), and High-Performance Liquid Chromatography (HPLC) of amino acids. The proximate analysis resulted in 71.16% of protein, 12.05% of water, 2.63% of carbohydrates, 2.21% of fat, 2.14% of crude fiber and 0.44% of ash. Mineral contents of Kian marine worm were 785.28 mg/kg potassium, 585.14 mg/kg magnesium, 180.98 mg/kg calcium, 0.89 mg/kg sodium and 0.44 mg/kg phosphorus. Kian marine worms contained 9 essential amino acids and 11 essential amino acids with levels of essential amino acids up to 206.94 mg/g and non-essential amino acids was 233.21 mg/g. Nutritional content in kian marine worms can be used as a potential high protein food resource.

Co-Authors Abda Abda Abdul Gofur Abdul Gofur Abdul Gofur Abdul Gofur Abu Husen Abu Husen Afifah Nur Aini Alif Yanuar Zukmadini Aloysius Duran Corebima Amin, Ihya Fakhrurizal Anton Setia Budi Ardiyas Robi Saputra Basuki B Purnomo, Basuki B Beno Wahono Beno Wahono, Beno Betty Lukiati Cicilia Novi Primiani Cicilia Novi Primiani Cicilia Novi Primiani Cicilia Novi Primiani, Cicilia Desy Putri Rahmawati Dewi Murni Dina Maulina Dwi Anggorowati Rahayu Endang Suarsini Endik Deni Nugroho Fahmi, Muhammad Najib Farizha Irmawati Farizha Irmawati, Farizha Fitri, Nurul Hadi Suwono Haqqi Anajili Setyanto Haqqi Anajili Setyanto, Haqqi Anajili Herawati Susilo Ihya Fakhrurizal Amin Ika Oktaviana Ika Oktaviana, Ika Istamar Syamsuri, Istamar Istamar Syamsyuri Izza Rohman Kurniawan Setia Putra Listawati, Rizki Mei Misgiati, Misgiati Mochammad Ali Mashur Moh. Amin Mohamad Amin Mohamad Amin Mohamad Amin Mohamad Amin Mohamad Amin Mohamad Amin Mohamad Amin Mohamad Amin Mohamad Amin Mohamad Amin Mohammad Amin Mohammad Amin Mohammad Amin Monalisa PJ Taihuttu Muh. Nur Akbar Murni Sapta Sari Nafisatuzzamrudah Nafisatuzzamrudah Nanda Earlia Nandang Mufti Naning Wahyuni Nindya Ulfa Wardhani Nugrahaningsih Nugrahaningsih Nur Hayati Rahman Fadli Rahmawati D Rahmawati Darussyamsu Reni Istiningrum Reni Istiningrum, Reni Ridho, Muhammad Andi Ali Ristiana, Evi Ritia Rahmawati Ritia Rahmawati, Ritia Rizka Elan Fadilah, Rizka Elan Rizki Mei Listawati Rizkia Suciati Rizkia Suciati Saparuddin Saparuddin Sembiring, Rinawati Siti Nur Arifah Siti Rahma Lestaluhu Sri Endah Indriwati Sri Rahayu Lestari Sulistyo Dwi Kartining Putro Sulistyo Dwi Kartining Putro, Sulistyo Dwi Kartining Susriyati Mahanal Sutiman B Sumitro Sutiman B Sumitro, Sutiman Sutiman B. Sumitro Ulin Nuha Ulya Ruwaida Umi Fitriyati Urla Tri Wulanzani Urla Tri Wulanzani, Urla Tri

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