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Journal : Jurnal Teknik Kimia dan Lingkungan

The Comparison of Free Cell and Immobilization Cell Fermentation on Bioethanol Production from Sorghum Stem by SSF and SHF Method Permanasari, Ayu Ratna; Syamfitri, Arum; Maharani, Devina; Setyaningrum, Sinta; Wibisono, Wahyu
Jurnal Teknik Kimia dan Lingkungan Vol. 8 No. 2 (2024): October 2024
Publisher : Politeknik Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33795/jtkl.v8i2.5755

Abstract

Bioethanol is a new and renewable energy source that can be produced from plants or crops containing sugars, starch, and lignocellulose. Sorghum stem contain a significant amount of sugar and lignocellulose. This study utilized sorghum stem in bioethanol production using Separated Hydrolysis and Fermentation (SHF) and Simultaneous Saccharification and Fermentation (SSF) methods. These two processes are generally used in the prouction of bioethanol with raw materials containing lignocellulose. However, raw materials derived from sweet sorghum stems has not been widely used in the previous studies. This study aimed to determine the effect of fermentation using free cell and cell immobilization techniques on various pH, as well as to identify the most optimal fermentation method (SHF or SSF) for producing the highest ethanol content in sorghum stem fermentation.  The fermentation was conducted at pH levels of 3, 4, and 5. Sorghum stem were processed into powder and followed by delingnification process by 3% of NaOH solution to degrade the lignin content. The hydrolysis process of sorghum stem used cellulase enzymes as the biocatalyst. Fermentation was carried out using Saacharomyce in term of dry yeast for 72 h. The results showed that the increasing within the range pH of 3-5 will increase the ethanol concentration. Freecell technique gave the better result over the immobilized. The best result reached out the ethanol concentration of 13.04 % by the SSF.
Co-Authors Adnan, Fahri Aji, Jul Fadli Anita Rahmawati Anita Rahmawati Anita Rahmawati Arsa, Sandi Alfa Wega Ayu Rahmawati AYU RAHMAWATI Danang Candra Wiratama Dianty Rosirda Eko Purwanto Entan Afriannisyah Erni Setiyorini Erni Setiyorini, Erni Ervi Suminar Fitria Nur Hamidah Fitria Nur Hamidah, Fitria Nur Frenky Arif Budiman Gustaji, F HANDAYANI Handayani Handayani Handayani Handayani Hesti Wahyuningtias Ika Agustina ika agustina Ika Agustina Ila Ageng Safiani Ita Noviasari Karisma, R F Kusnianto, Maratus Sholichah Fitriah Hajar Kuswidyarti, Yulia Laily Prima Monica Laily Prima Monica Laily Prima Monica, Laily Prima Lely Indah Kurnia Lely Indah Kurnia, Lely Indah Levi Tina Sari Levi Tina Sari, Levi Tina Lia Rahmawati Lia Rahmawati, Lia Lury Trijayanti Maharani, Devina Maratus Sholichah FHK Maratus Sholichah Fitriah Hajar Kusnianto Maria Ulfa Maria Ulfa Mar’atus Sholichah Fitriah Hajar Kusnianto Muhammad Muchlisin Muhammad Muchlisin, Muhammad Nevy Norma Renityas, Nevy Norma Ning Arti Wulandari Noviasari, Ita Nurun Nikmah Okky Cintya Permata Dewi Permanasari, Ayu Ratna Prima Monica, Laily Putri, Raden Roro Dewi Rahmawati Aktyani Raden Roro Dewi Rahmawati Aktyani Putri Rahmawaty, RR Dewi Rifadil, Mochammad Machmud Robby Sudarman Safiani, Ila Ageng Sari, Ita Novia Setyaningrum, Sinta Shinta Wulandari, Shinta Sholichah F.H.K., Maratus Syamfitri, Arum Thatit Nurmawati, Thatit Tina Sari, Levi Trijayanti, Lury Ulfa Husnul Fata Wahyuningtias, Hesti Wahyuningtias, Hesti Widyanarti, Tantry Wiga, Sandi Alfa Wiratama, Danang Candra Yeni Kartika Sari, Yeni Kartika Yulia Kuswidyarti Yulistiani Yulistiani, Yulistiani Yunandar, Fikri