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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kedokteran Brawijaya The Journal of Experimental Life Sciences (JELS) Research Journal of Life Science Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Jurnal Fish Protech Jurnal Kedokteran Hewan
Edi Widjajanto
Laboratorium Patologi Klinik Fakultas Kedokteran Universitas Bwawijaya Malang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science Immunology & microbiology Medicine & Pharmacology Neuroscience Veterinary

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PENINGKATAN EKSPRESI INTERLEUKIN (IL)-4 BERHUBUNGAN DENGAN PENURUNAN BAX DAN APOPTOSIS LIMFOSIT PADA BRONKIOLUS DAN PARU MENCIT ASMA Yuliarto, Saptadi; Kusuma, HMS Chandra; Widjajanto, Edi
Jurnal Kedokteran Brawijaya Vol 25, No 3 (2009)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (917.17 KB) | DOI: 10.21776/ub.jkb.2009.025.03.2

Abstract

Decrease of lymphocyte  apoptosis is  one factor  that  leads  to  chronic airway inflammation  in  allergic asthma. Previous  studies  revealed  the  role  of  interleukin  (IL)-4  in  preventing  lymphocyte  apoptosis;  however  there  are few  studies  about  the  role  of  lymphocyte-Bax  and  its  relationship  with  IL-4  in  asthma.  The  aim  of  the  study  is  to demonstrate IL-4 role in preventing lymphocyte apoptosis via lymphocyte-Bax inactivation in bronchioli and lungs  of  asthmatic  mice.  It  is  a  randomized  control  group  experimental  study .  The  subject  was  Balb/c  mice  that categorized  into  2  groups:  asthma  and  non-asthma.  Asthma  group  was  sensitized  by  ovalbumin intraperitoneally  in  day  0  and  14,  followed  by  inhalation  every  2-3  days  for  6  weeks.  In  week  8,  all  of  mice were terminated.  The  IL-4  and  lymphocyte-Bax  expression  was  examined  through  immunohistochemistry  method, whereas  lymphocyte  apoptosis  by  TUNEL  method.  Independent  sample  t-test,  Mann  Whitney  U  test, regression  analysis,  and  path  analysis  was  used  in  statistical  analysis  with  confident  interval  95%.  The bronchioli and lungs specimens were obtained from 18 mice (9 in each group). Lymphocyte apoptosis was similar between 2 groups (p=0.1 16), additionally lymphocyte-Bax  decreased in  asthmatic group (p=0.003). This  indicated  low  activity  of  lymphocyte  apoptosis  in  asthmatic  group.
Mastosit Perivaskuler Berperan Sebagai Vasodilator Pembuluh Darah pada Mesenterium Tikus Ibrahim, Mohamad Nur; Soleh, Miftahu; Widjajanto, Edi
Jurnal Kedokteran Brawijaya Vol 25, No 3 (2009)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1004.696 KB) | DOI: 10.21776/ub.jkb.2009.025.03.8

Abstract

Mastocyt  degranulated  can  cause  vasodilatation  of  blood  vessels,  but  it  is  unclear  whether  there  is  a relationship  between the  number of mastocyt with a diameter of blood vessels. This study aims to  determine the  relationship  between the  number of mastocyt pherivascular and diameter of blood vessels.  This research is  in  vivo  exploratory  research  in    Wistar  rat  mesentery which was sensitated  with ovalbumin.  The  diameter  of blood  vessels  at  mesentery  preparate  were  measured  microscopically  using  a  micrometer  scale  standards.  At large  diameter  of  blood  vessels   (2.860  ±  0.348),  the  number  of  mastocyt was 12.3  ±  2.3.  At  medium diameter of blood vessel (1.276 ± 0.198), the  number of mastocyt was 4.4 ± 0.5. Blood vessels with small diameter (0.961 ± 0.043), the  number of mastocyt was 3.5 ± 1.3. Calculations area on the  microscope visual field  was divided into  four  areas (counting area), there  were zone 1, zone 2, zone 3 and zone 4. Statistical analysis showed  that  the  correlation  between  the  number  of  mastocyt  at   zone  1  and  the  diameter  of  blood  vessels  was 0,904 at 1% significance level.  Similar to  that  at zone 2 (r=0,892),  zone 3 and zone 4 (r=0,940).  We conclude there  was  a  significant  relationship  between  the  number  of  mastocyt  and  the  diameter  of  blood  vessels. Keywords:  mastocyt  pherivascular,  diameter  of  blood  vessels
PENGARUH PEMBERIAN MINYAK BUAH MERAH (Pandanus conoideus) TERHADAP STRES OKSIDATIF SEL ENDOTEL YANG DIPAPAR DENGAN SERUM PENDERITA MALARIA FALCIPARUM DAN NETROFIL INDIVIDU SEHAT Armiyanti, Yunita; Fitri, Loeki Enggar; Widjajanto, Edi
Jurnal Kedokteran Brawijaya Vol 23, No 1 (2007)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1699.473 KB) | DOI: 10.21776/ub.jkb.2007.023.01.2

Abstract

Complication in Plasmodium falciparum malaria is associated with endothelial damage and overproductionof free radicals (oxidative stress) by activated neutrophils and endothelial cells. Red fruit (Pandanus conoideus) oil  has a very high antioxidant content, therefore it may neutralize free radicals and prevent endothelial damage. The aim of this research is to prove that red fruit (Pandanus conoideus) oil reduce Reactive Oxygen Intermediate (ROI) production  of endothelial cells exposed to severe malaria patient serum and neutrophils from healthy donor. Endothelial cells from human umbilical veins were coincubated with serum from severe malaria patient and with neutrophils from healthy donor  (positive control group). HUVEC’s normal was used as negative control,  where as  the experimental groups were given with red fruit oil in different concentrations (2.8%, 5.7% dan 11.3% ). The Reactive Oxygen Intermediate (ROI) production of  endothelial cells was semiquantitatively measured by using NBT-reduction assay and the score of ROI was counted. The results were statistically analyzed with ANNOVA (p<0.01). The rate of ROI production of endothelial cells was markedly increased after incubation with patient serum and neutrophils.
Perbandingan Uji Kepekaan Antibiotika Metode Primary Sensitivity Test dengan Metode Baku terhadap Isolat Bakteri dan Pus Dwidjatmika, Heri; Widjajanto, Edi; Sumarno, Sumarno
Jurnal Kedokteran Brawijaya Vol 18, No 3 (2002)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3098.057 KB)

Abstract

Pengobatan terhadap penyakit infeksi sebaiknya didasarkan atas hasil tes kepekaan antibiotika. Tes kepekaan antibiotika yang biasa dikerjakan di laboratorium sentral seksi mikrobiologi Klinik RSSA Malang yang sudah dibakukan adalah selalu didahului oleh kultur bakteri. Untuk mengetahui hal tersebut memerlukan waktu yang cukup lama yakni anatar 3 sampai 4 hari. Metode Primary Sensitivity Test (PST) adalah metode tes kepekaan bakteri terhadap antibiotika yang hasilnya dapat diketahui dengan cepat yaitu 1 hari. Penelitian ini tujuannya adalah untuk mencari senditifitas dan spesivitas dari tes kepekaan metode PST dibandingkan dengan hasil tes kepekaan metode baku pada sampel pus. Pada spesimen pus metode PST dikerjakan dengan cara langsung dari bahan pemeriksaan. Spesimen pus digoreskan pada medium Disk Sensitivity Test (DST), yang selanjutnya pada permukaan medium DST tersebut diletakkan kertas yang berbentuk cakram yang didalamnya mengandung antibiotika tertentu. Hasil dari tujuan penelitian dianalisis dengan menggunakan program komputer Cat Maker. Hasil penelitian ini menunjukkan bahwa senditifitas tes kepekaan metode PST antibiotika amikacine, cefotaxime, ciprofloxacine, dan amoxicillin masing-masing berurutan adalah 77%, 71%, 74%, 64%, dan 35%. Hasil spesivitas tes kepekaan metode PST antibiotika  amikacim, cefotaxime, cefuroxime, ciprofloxacine, dan amoxcillin masing-masing berurutan adalah 83%, 85%, 85%, 85%, dan 90%. Kesimpulan dari hasil penelitian metode PST maka antibiotika amikacine, cefotaxime, dan cefuroxime dapat dijadika sebagai pegangan dalam penanganan penderita luka infeksi secara dini. Akan tetapi dalam penerapan klinik masih perlu penelitian lebih lanjut mengenai penggunaan hasil tes kepekaan metode PST.
PERANAN MAKROFAG PADA PROLIFERASI, DIFERENSIASI DAN APOPTOSIS PADA PROSES HEMATOPOISIS (PENELITIAN PADA LIMPA JANIN TIKUS DAN ASPIRAT SUMSUM TULANG MANUSIA) Widjajanto, Edi
Jurnal Kedokteran Brawijaya Vol 21, No 1 (2005)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1346.822 KB) | DOI: 10.21776/ub.jkb.2005.021.01.6

Abstract

Macrophage stimulates growth of surrounding cells by various cytokines and growth factors synthesized  and eliminate apoptotic cells by its phagocytes activity. This study was to evaluate the evidence of macrophage as regulator cells during haematopoiesis. Haematopoietic activity assessed microscopically by proportion of various haematopoietic blood cells to total nucleated blood cells on imprint preparation of fetal ratus (Wistar) spleen and human marrow aspirate, after Wright’s stain coloration. Fetal ratus spleen and human marrow aspirate were used to evaluate macrophage as a stimulator cell and suppressor cell respectively. During prenatal period, the proportion of macrophage increased at 14thdays (1.3 ± 0.9%), 16thdays (7.6 ± 1.8 %), 18thdays (12.9 ± 2.8 %) and 20thdays prenatal gestation periods (14.1 ± 3.1%). Thismacrophage increment was associated with the increase of other blood cells, except the proportion of lymphoid cell which decreased. During postnatal period the macrophage proportion decreased (11.0 ± 1.7 %), and other bloodcells proportion continuously increased except lymphoid cells which decreased. Many haematopoietic islands representing active haematopoietic process were found at 14thdays of fetal rat spleen. Macrophage in human hypocellular marrow (n = 9) increased and it exceed other blood cells proportion (29 %, p < 0.001) and contained phagocytosed apoptotic bodies. This study concludes that high macrophage regulates  haematopoiesis when it was proportionally low and regulate haematopoiesis when it was proportionally high. Keywords:Macrophage, Apoptosis, Haematopoiesis
HUBUNGAN ANTARA PENINGKATAN KADAR LEKOTRIEN B4 (LTB-4) DENGAN HIPOSELULARITAS SUMSUM TULANG (PENELITIAN PADA ASPIRAT SUMSUM TULANG) Widjajanto, Edi
Jurnal Kedokteran Brawijaya Vol 21, No 2 (2005)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2005.021.02.2

Abstract

ABSTRACT Previous study on human bone marrow culture showed that leukotrien B-4 (LTB-4) which was added to granulocyte-macrophage and erytroid colony, suppressed the growth of colonies. This study assessed the correlation between LTB-4 and marrow cellular. Marrow aspirate which was taken from 60 adult patients (26 men and 34 women) suffering from various haematopoietic problems was examined for amount of LTB-4 and the degree of marrow cellular. ELISA principle was applied for LTB-4 measurement and marrow cellular assessed by counting the member of nucleated cells per cubic-mm of marrow aspirate using microscopic counting chamber principle. Existing difference of the strength of correlation of LTB-4  and  marrow  cellular  was  analized  using  manova and  path  analysis  and  expressed  as  path  coefficient.The difference of confounding factors such as gender, age and type of diseases (haematopoitic problems) were controlled and homogenized during statistical analysis using SPSS 10 program. Path coefficient in the multi cellular marrow (n = 60) was – 0.092 (p = 0.495) and path coefficient in hypo cellular marrow (n = 28) was – 0.406 (p = 0.013). This study concludes that the increase of amount of LTB-4 in hypo cellular marrow is associated with the decrease of marrow cellular. Keywords : Leukotrien B-4, Hypocelular marraw, Apoptosis, Hematopoisis
Karboksimetil Kitosan Menurunkan Degranulasi Mast Cell yang Diinduksi Oleh Ovalbumin Ibrahim, Mohamad Nur; Widjajanto, Edi; Permatasari, Nur; Sabarudin, Akhmad
Jurnal Kedokteran Brawijaya Vol 25, No 1 (2009)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (165.792 KB) | DOI: 10.21776/ub.jkb.2009.025.01.2

Abstract

ABSTRACTOvalbumin is known as mast cell degranulating agent through the FCεRI aggregation. Carboxymethylchitosan (CMCs), a biocompatible cationic polymer was evaluated to reduces mast cell degranulation inducedby ovalbumin. In this experiment, wistar rats were darboxymethyl chitosanivided into 5 groups which consist of 1 control group and 4allergen groups. Among the allergen groups, 3 groups were treated with carboxymethyl chitosan/CMCs(doses : 0,25 mg, 0,50 mg and 1,00 mg) for 22 days. The result showed a significant (P<0,01) reduction inmast cell degranulation in allergen groups. This finding indicate that CMCs possesses antiinflammationactivity mediated by reducing of mast cell degranulation.Keywords : ,c mast cell degranulation
PENGARUH PEMBERIAN EKSTRAK BIJI JINTEN HITAM (Nigella sativa) TERHADAP KADAR GSH, MDA, JUMLAH SERTA FUNGSI SEL MAKROFAG ALVEOLAR PARU TIKUS WISTAR YANG DIPAPAR ASAP ROKOK KRONIS Marwan, Marwan; Widjajanto, Edi; Karyono, Setyawati
Jurnal Kedokteran Brawijaya Vol 21, No 3 (2005)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (380.463 KB) | DOI: 10.21776/ub.jkb.2005.021.03.2

Abstract

Cigarette smoke is the main risk factor of chronic  obstructive pulmonary disease (COPD). Several studies about COPD has focused on the oxidative stress in lung. Oxidative stress was related to inactivation of antiprotease enzymes, airway epithelial destruction and proinflammatory genes expression. Antioxidant may useful for treatment of COPD. Black seed (Nigela sativa) studies have showed that black seedhas antioxidant activity. The aim of this research  was to prove that black seed crude extract can protect wistar rat lung from oxidative stress which was caused by chroniccigarette smoke exposure. This experimental research used randomizedcomplete design with control group. Thirty wistar strain of Rattus novergicus, 2.5 to 3 months old, 150 – 250 grams ofweight were divided into five groups. First group  was used as negative control group (without any treatment), second group as positive control group (only cigarettesmoke exposure treatment), third group as cigarette smoke exposureand black seed crude extract dose 0.6 gr/kilogram  of body weight/day (group A), cigarette smoke exposure and  black seed crude extract dose 1.2 gr/kilogram of body weight/day (group B) and cigarette smoke exposure and black seed crude extract dose 2.4 gr/kilogram of body weight/day (group C). After 3 months five parameters were measured in this research.
Keseimbangan Sitokin Th1/Th2 Berperan Dalam Proliferasi Mastosit Jaringan Paru Pasca Pemberian Diet Tinggi Lemak pada Tikus Rosita, Rita; Indra, Muhamad Rasjad; Widjajanto, Edi
Jurnal Kedokteran Brawijaya Vol 25, No 1 (2009)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (928.411 KB) | DOI: 10.21776/ub.jkb.2009.025.01.3

Abstract

ABSTRACTAsthma and obesity are prevalent disorders, each with a significant public health impact, and a large andgrowing body of literature suggests an association between the two. The systemic inflammatory milieu inobesity leads to metabolic and cardiovascular complications but this environment alters asthma risk orphenotype is not yet known. Studies that evaluated the effects of leptin and obesity on airway inflammationin response suggest that air way inflammation is enhanced by leptin. Leptin is stimulate a Th1 cytokineprofile and suppresses Th2 cytokine production. Whether resistance to the effects of the lung or on immunefunction is unknown, but such resistance might be expected to polarize to a Th2. The aim of this study isidentify effect of leptin to lung mast cell proliferation via shifting Th1/Th2 balance using diet-induced obesemice. Because there are contradictive between leptin immune function in leptin deficient and obese, tounderstand the effect of leptin in diet-induced obese mice on immune function, lung mast cell proliferationand correlation with IL-4 as represent cytokine Th2 and IL-2 represent cytokine Th1 has been studied.
FAKTOR DOMINAN DALAM MEMPREDIKSI MORTALITAS PASIEN DENGAN SEPSIS DI UNIT GAWAT DARURAT Akbar, Ilham; Widjajanto, Edi; Fathoni, Mukhamad
Jurnal Kedokteran Brawijaya Vol 30, No 2 (2018)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2018.030.02.14

Abstract

Mortalitas pasien dengan sepsis meningkat secara drastis dengan tingkat keparahannya. Ada banyak faktor yang yang menjadi pertanda perburukan kondisi maupun kematian pasien dengan sepsis. Seorang perawat diharapkan dapat menjadi lini terdepan di unit gawat darurat untuk dapat memprediksi mortalitas pasien dengan sepsis untuk menentukan tindakan definitif dengan segera tanpa melakukan pemeriksaan laboratorium. Tujuan penelitian yaitu menganalisis faktor dominan yang berhubungan dengan mortalitas pasien dengan sepsis. Penelitian kuantitatif ini menggunakan desain observasi analitik dengan pendekatan retrospektif. Sampel menggunakan rekam medis pasien dengan sepsis disesuaikan dengan kriteria inklusi dan ekslusi dan ditentukan dengan teknik consecutive sampling yaitu sebanyak 75 responden. Analisis bivariat yang dilakukan pada penelitian ini menggunakan kontigensi lambda dan chi square, sedangkan analisis multivariat menggunakan regresi logistik. Hasil uji bivariat menunjukkan bahwa usia (r=0,305, p=0,030), nilai qSOFA (r=0,678, p=0,000), dan penyakit komorbid (r=0,243, p=0,030) masing-masing memiliki arah hubungan positif dengan mortalitas pasien sepsis. Hasil regresi logistik menunjukkan penyakit komorbid meningkatkan 6,6 kali mortalitas pada pasien sepsis (OR=7,000, p=0,016). Penyakit komorbid dan nilai qSOFA adalah faktor yang dapat mempredikisi mortalitas pasien sepsis namun penyakit komorbid merupakan faktor yang paling dominan. 
Co-Authors A. Harijono Abdalrauf A Mahmud Yousif, Abdalrauf A Mahmud Akhmad Sabarudin Anik Puryatni ary yunanto Astari, Lina Cahyono, Haryudi Cahyono, Haryudi A. Chomsa Dintasari Umi Baszary desy wulandari Dhaniar Mudita, Dhaniar Diana Lyrawati Djanggan Sargowo Dwidjatmika, Heri Elhemeidy, Rehab Mohammed Mustofa Erni Yudaningtyas Fitriyani, Delfi Harjoedi Adji Tjahjono, Harjoedi Adji Hidayat Sujuti HMS Chandra Kusuma Ibrahim, Moh. Nuh Ilham Akbar Karyono Mintaroem Komaruzzaman, Abdur Razaq Lilik Supriati, Lilik Loeki Enggar Fitri M Aris Widodo Marwan Marwan Mas Mansyur, Mas Mayasari, Destianti Indah Mayasari, Destianti Indah Miftahu Soleh Moch Sasmito Djati Mohamad N Ibrahim Mohamad Nur Ibrahim MS Chandra Muhamad Rasjad Indra Mukhamad Fathoni Mulyohadi Ali Noerpuspita, Putri Nur Permatasari Oski Illiandri Poerwosusanta, Hery Prawiro, Sumarno Reto Putri, Agustine Hanafi Rias Gesang Kinanti Rifa’i, Muhaimin Rinik Eko Kapti, Rinik Eko Rita Rosita Rosdiana, Yanti Saptadi Yuliarto Setyawati Karyono Sofiyatin Reni Sumarno . Suryanto, Tjahyo Sutiman B. Sumitro Yunita Armiyanti Zairin Noor