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Hendra Wijaya
Center for agro-based Industry
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Journal : Biopropal Industri

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PREDIKSI STRUKTUR TIGA DIMENSI PROTEIN ALERGEN PANGAN DENGAN METODE HOMOLOGI MENGGUNAKAN PROGRAM SWISS-MODEL - (Prediction of Three-Dimensional Structure from Food Allergen Protein Through Homology Method Using SWISS-MODEL Program) Wijaya, Hendra; Hasanah, Fitri
Biopropal Industri Vol 7, No 2 (2016)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

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Abstract

Information about three-dimensional structure of protein is important to clearly understand its character and function in molecular level. Protein structure determination through laboratory testing needs highly cost of steps and instrument. Three dimensional structure of protein can be scientifically predicted using several in silico methods. The structure of allergen protein was predicted using SWISS-MODEL as homology method in this research. The result showed that structure prediction of allergen protein B7TWE7 (UniProt ID) from Fagus sylfatica (beechnut) have three-dimensional structure with identity value 72% and e-value = 3,4x10-58. This study used the 1e09A (PDB ID) of Prunus avinus (cherry) as a template.Keywords: allergen, homology method, protein structure, swiss-model ABSTRAKInformasi struktur tiga dimensi protein penting untuk memahami sifat dan fungsi protein ditingkat molekular secara detil. Penentuan struktur protein dengan cara pengujian di laboratorium memerlukan tahapan dan instrumentasi dengan biaya tinggi. Struktur tiga dimensi protein dapat diprediksi secara ilmiah dengan menggunakan beberapa metode secara in silico. Metode yang digunakan pada penelitian ini adalah metode homologi menggunakan program SWISS-MODEL. Protein yang akan diprediksi struktur tiga dimensinya adalah protein alergen pangan. Hasil penelitian menunjukkan bahwa prediksi struktur protein alergen B7TWE7 (UniProt ID) dari Fagus sylfatica (beechnut) dengan metode homologi menggunakan program SWISS-MODEL menghasilkan model struktur tiga dimensi protein dengan nilai identity 72% dan e-value = 3,4x10-58. Template protein yang digunakan adalah 1e09A (PDB ID) dari Prunus avinus (cherry).Kata kunci: alergen, metode homologi, struktur protein,  swiss-model
Co-Authors Abdul Syukur Ade Pratama Aliya Nur Hasanah Amir Amir Anabella, Christella Norica Andina, Ni Kadek Devi Pradnya Angelina, Ariyanthini Elisabeth Anggreini, Dwi Desi April Gunawan Malau Ardhani Dwi Lestari, Maria Lucia Arista Candra Irawati Bambang Widjaja Berliani, Annisa Butar-Butar, Mangasi Calvin Calvin Choirunisa, Syifa Dandy Pramana Hostiadi Darmawan, Fiki Dwi Dewi Isadiartuti Dewi, Ni Putu Intan Satya Dwi Budi Santoso Eka Indra Setyawan ernikawati, ernikawati Evi Triandini Felix Pasila Fitri Hasanah Gesti Memarista Gunawan, Erick Teofilus Haidir, Hala Handry Khoswanto Hutagaol, Jarunjung Intan Permatasari Jus'at, Idrus Katili, Yusril Kiwo, Diminus Lazuarni, Shafiera Lestari, Fitriana Dwi Lucy Simorangkir Lukman Hakim M Yusuf Marjono, Cornelia Clarissa Mastar Asran Miradz, Arik Moito, Febrianti Moonti, Roy Marthen Motik, Chandra Muhamad Azhar, Muhamad MUNAJIR, ACHMAD Naripradnya, Putu Saradevi Ngabito, Meriyanti Nurkholis, Kgs. M . Panigoro, Nurmaya Parta, I Putu Rizki Karisma Pranata, Andri Pratama, Wahyu Riski Putri, Anastasia Aurelia Rahayu, Leni Ramadhan, Moh. Fauzan Rini Setiawati Rizky Nugroho Adji Rosiana, Febe Rudiyanto Rudiyanto, Rudiyanto S. Patricia Febrina Dwijayanti Samvara, Made Adhiguna Santoso Ratu, Dennis Setiasih Setiasih, Setiasih Sienny Thio Sofian, Sofian Sukmawati . Sukri, Tony Surjaatmaja, Lukas Susanti, Aprillia Nanda Susanto, Hendro Sutri, Wiratama Kadek Thaib, Abdul Shamad Virginia, Maria Vivi Bachtiar Wahyuning Astuti, Reny Winisudo, Christian Kevin Wira Udytama, I Wayan Wahyu Yanti, Rina Dwi Yogi Yasa Wedha Yoraeni, Ani