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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Journal of Degraded and Mining Lands Management AGRIVITA, Journal of Agricultural Science Jurnal Agroteknos Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat PROSIDING SEMINAR NASIONAL AGRIBISNIS CROP AGRO, Scientific Journal of Agronomy Jurnal Agercolere Berkala Penelitian Agronomi Jurnal Agrium Anoa : Jurnal Pengabdian Masyarakat Sosial, Politik, Budaya, Hukum. Ekonomi Jurnal Pengabdian Masyarakat Ilmu Terapan (JPMIT) LEBAH Jurnal Pengabdian Nusantara (JPN) Studia Ekonomika
Teguh Wijayanto
Universitas Halu Oleo
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INDUKSI TUNAS GADUNG (Diocorea hispida Dennst) SECARA IN VITRO ARIF, NORMA; ANSI, AZHAR; WIJAYANTO, TEGUH
Jurnal Agroteknos Vol 4, No 3 (2014)
Publisher : Jurnal Agroteknos

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Abstract

Yam (Dioscorea hispida Dennst) is a tuber crop that belonges to food sources and has not been widely recognized by the public. Aside from being a food ingredient, it can also be used as a natural pesticide. Until now, efforts to use the potential of yam have not been handled well. This research was conducted at the In vitro Laboratory of the Faculty of agriculture, Haluoleo University, from May to July 2014. The purpose of the study was to obtain the proper concentration of BAP to induce yam plant shoots in vitro. The research design used was a completely randomized design with 5 replications. One segment of the stem explants were grown on MS basic medium with different concentrations of BAP (0.5-4 ppm), with 5 replications and each replication consisted of 1 bottle containing 2 explants. Data were analyzed using Duncan's Multiple Range Test (DMRT). The research results showed that the addition of various concentrations of BAP affected the span of the emergence of the first shoots but did not affect the percentage of explants alive and growing shoots. BAP concentration of 2 mg / L was the optimal concentration to induce shoots of yam (Dioscorea hispida) with a span began to shoot appearance at week 4 after planting.
ANALISIS VARIABILITAS KULTIVAR JAGUNG PULUT (Zea mays Ceritina Kulesh) LOKAL SULAWESI TENGGARA SAFUAN, LA ODE; BOER, DIRVAMENA; WIJAYANTO, TEGUH; SUSANTI, NELI
Jurnal Agroteknos Vol 4, No 2 (2014)
Publisher : Jurnal Agroteknos

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The experiment was conducted in the Rahandouna village, Poasia, Kendari Southeast Sulawesi, from August to November 2013.  The purpose of this study was to determine the estimate of heritability between characters of thirteen local waxy corn cultivars of Southeast Sulawesi. This study was prepared using a randomized block design (RBD), with 3 replicates. Total waxy corn cultivars studied was 13 species, so that there were 39 plots. Each plot consisted of a single cultivar. Observed variables were plant height (cm), stem diameter (cm), leaf area (cm2), number of leaves (strands), ear length (cm), cob diameter (cm), number of rows per ear, weight of 100 seeds (g), ear weight. The results of this study showed that there was narrow variability on all local waxy corn characters observed. Keywords: Local waxy corn, characters, cultivars, Southeast Sulawesi, variability
EXAMINATION OF CHIMERIC GENETIC STRUCTURE OF T0 TRANSFORMED LUPIN SHOOTS Wijayanto, Teguh
Jurnal Agroteknos Vol 1, No 1 (2011)
Publisher : Jurnal Agroteknos

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Abstract

Kurang  responsifnya eksplan  terhadap  medium  invitro  dan  efisiensi  transformasi yang relatif rendah merupakan kendala umum yang dihadapi pada transformasi genetik tanaman legum.   Pada tanaman lupin, satu spesies legum, sebagian besar klon yang diperbanyak dari satu tunas transgenik (single transformation event) sering tidak mengandung gen yang ditransfer (transgene), dan menghasilkan keturunan yang tidak transgenik, walaupun awalnya plantlet tersebut terbukti transgenik. Kimera (chimerism) diduga sebagai penyebab dari penyimpangan segregasi gen (reduced transgene segregation) ini.  Penelitian ini mencoba untuk membuktikan isu kimera tersebut. Uji in-situ PCR dan uji histokemikal GUS & lokalisasi selular dari transgen (dalam hal ini gen gus) dilakukan untuk mengetahui apakah dan dimana transgen tersebut terekspresi pada tunas lupin trasngenik generasi T0  tersebut.   Uji in-situ PCR dan histokemikal GUS menunjukkan bahwa GUS umumnya terekspresi pada beberapa bagian dalam batang tunas (plantlet T0) dan tidak tersebar merata. Hasil ini memberikan bukti struktural yang penting bahwa transgen terekspresi  secara  sektorial  pada  tunas  lupin  generasi  T0.    Ini  sekaligus  membuktikan bahwa tunas lupin generasi T0  benar-benar kimera, dan menjadi penyebab penyimpangan segregasi transgen pada generasi T1.   Upaya-upaya perlu dilakukan untuk mengurangi kejadian kimera ini, sekaligus mendapatkan ekspresi gen yang solid pada tunas (plantlet) lupin (solid transformant). Key words:   Chimera, transgene segregation, in-situ PCR, GUS histochemical assay
PENGELOMPOKAN PATOTIPE Xanthomonas oryzae pv. oryzae ASAL SULAWESI TENGGARA MENGGUNAKAN PADI GALUR ISOGENIK IRRI Syair, H; Samirin, Samirin; Wijayanto, Teguh; Khaeruni, Andi
Jurnal Agroteknos Vol 2, No 1 (2012)
Publisher : Jurnal Agroteknos

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Abstract

This Research aimed at grouping of Xanthomonas oryzae pv. oryzae pathotypes in rice cropping center of Southeast Sulawesi using isogenic lines of IRRI, as well as to know the Xanthomonas oryzae pv. oryzae pathotypes dominating and spreading in rice cropping center of Southeast Sulawesi. The pathotype grouping was determined by leaf innoculated cutting method using 30 isolates of Xanthomonas oryzae pv. oryzae from Southeast Sulawesi in 10 isogenic lines, namely IRBB1, IRBB2, IRBB3, IRBB4, IRBB5, IRBB7, IRBB10, IRBB11, IRBB13, and IRBB21. Each isolate was inoculated on the 10 isogenic lines and repeated twice. Observation  of disease intensity was conducted randomly on five leaves at four weeks after inoculation. The disease intensity was measured by the ratio of the length (cm) of leaf blight symptom to total the length of leaf sample (cm). The research results indicated there were seven pathotypes of Xanthomonas oryzae pv. oryzae in Southeast Sulawesi, i.e : pathotype X, XI, XII and four new pathotypes. Pathotype XII was the dominant pathotype, abort 79.31% of the total isolates and spreading in three regencies, Kolaka, Konawe, and South Konawe. Keywords: bacterial leaf blight, Xanthomonas oryzae pv. oryzae, pathotype
PERBANYAKAN BIBIT JERUK SECARA IN-VITRO DAN BEBAS PENYAKIT CVPD Wijayanto, Teguh; Taufik, Muhammad; Boer, Dirvamena; Suliartini, Ni Wayan Sri
Jurnal Agroteknos Vol 1, No 3 (2011)
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Abstract Citrus Vein  Phloem Degeneration (CVPD) is  one  of  the  most important diseases of citrus. Worldwide and national yield losses of citrus production due to this disease infection have been very significant.   This research ultimately aimed at producing citrus stocks through in-vitro culture, and free of CVPD.  Citrus seeds were used as explant and cultured on MS and WPM in-vitro media, supplemented with malt extract. Citrus plantlets were tested for the presence of  CVPD DNA using CVPD specific primers in PCR reactions.   Research results showed that MS basal medium supplemented with malt extract was quite good for in- vitro production of citrus plantlets. Plantlets were negative for CVPD infection based on PCR tests.  CVPD-free seedlings (Citrus reticulata) have been grafted with citrus rootstock (Citrus sinensis).  Grafted citrus seedlings were also proven to be negative for CVPD infection based on similar PCR tests.  Citrus seedlings/stocks produced by in-vitro culture, and free of CVPD, are now available for further growth. Keywords: Citrus Vein Phloem Degeneration (CVPD), in-vitro culture, PCR
KERAGAMAN GENETIK BEBERAPA CABAI RAWIT (Capsicum frutescens L.) LOKAL ASAL SULAWESI TENGGARA SUDIARSIH, TIRA; BOER, DIRVAMENA; HADINI, HAMIRUL; SUTARIATI, GUSTI AYU KADE; ANIMA HISEIN, WAODE SITI; WIJAYANTO, TEGUH
Jurnal Agroteknos Vol 13, No 3 (2023)
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Abstract

This study aims to determine the genetic diversity of several local cayenne pepper cultivars from Southeast Sulawesi and to find out which local cayenne pepper cultivars from Southeast Sulawesi show the best growth and yields. This research was carried out at the Agricultural Extension Center (BPP) Pilot Land located in Langkoroni Village, Maligano District, Muna Regency. The research implementation time starts from October 2021 to February 2022. Data analysis using variety fingerprints, if it shows a real and very real influence then continued Duncan multiple range test (DMRT) with the help of statistical product and service solution (SPSS) application facility and calculate genetic diversity value. The results showed that quantitative characters with high diversity were found in the characters of flowering age, harvest age, number of fruits, fruit weight, fruit length and fruit diameter. Somewhat high is found in the character of plant height and leaf area. Slightly low was found in the character of the number of leaves and low was found in the character of the length of the fruit stalk. The qualitative characters observed were uniform because there were no differences in the observed local cayenne pepper cultivars from Southeast Sulawesi. The local cayenne pepper cultivar from Southeast Sulawesi that showed the highest growth and fruit yield was found in the cultivar from Kolaka (145.96 grams/plant), followed by North Buton-2 (125.71 grams/plant), then South Konawe (121.93 grams/plant) compared to other local cayenne pepper cultivars.
RESPON FASE PERTUMBUHAN BEBERAPA GENOTIPE JAGUNG LOKAL SULAWESI TENGGARA TERHADAP KONDISI KEKURANGAN AIR Wijayanto, Teguh; Sadimantara, Gusti Ray; Etikawati, Made
Jurnal Agroteknos Vol 2, No 2 (2012)
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The  research was  conducted to  test  the  potency  of  several local  maize genotypes (landraces) of Southeast Sulawesi and to find the genotypes that were tolerant to less water conditions. The research was performed in  a  plastic house at  the  experimental farm of Agriculture Faculty, University of Halu Oleo.  The research was arranged in a Randomized Complete Design (RCD) with 2 treatment factors. The first factor was water availability level (A), consisted of 3 (three) levels (A0, A1 dan A2), and the second factor was maize genotype, consisted of 9 local maize and 1 national variety (Arjuna).  Research results showed that the less water conditions (A1 and A2) had negative effects on maize vegetative variables (plant height, plant diameter, leaf number and length of leaf tip).  On the other hand, the genotype treatment had significant effect on plant height, plant diameter, and length of leaf tip. Genotipe G7 dan G6 had the highest plant height, while G1 had the biggest plant diameter. Keywords: less water condition, local maize genotype, vegetative stage
PENGARUH PEMBERIAN BERBAGAI JENIS PUPUK KANDANG TERHADAP PRODUKSI TANAMAN KACANG HIJAU (Vigna radiata L. NUR ALAMSYAH, MUHAMAD; SABARUDDIN, LAODE; NURMAS, ANDI; WIJAYANTO, TEGUH; ARSYAD, MIRZA ARSIATY; SAFUAN, LA ODE
Jurnal Agroteknos Vol 13, No 1 (2023)
Publisher : Jurnal Agroteknos

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Abstract

This study aims to determine the effect of the application of various types of manure on the production of mung bean. The research was carried out at the Field Laboratory of Experimental Gardens II, Faculty of Agriculture, Halu Oleo University, Kendari, from October to December 2021. The study used a randomized block design consisting of four treatments, namely control (P0), cow manure 10 t ha-1 (P1), goat manure 10 t ha-1 (P2) and chicken manure 10 t ha-1 (P3). Each treatment was repeated 4 times so that there were 16 experimental units. Variables observed were number of pods, pod weight, seed weight, weight of 100 seeds, seed production ton ha-1, analyzed by variance. If the variance shows a significant effect, then proceed with the Least Significant Difference Test (BNT) at the 95% confidence level. The results showed that the application of manure can increase the production of mung bean plants. Treatment of chicken manure 10 t ha-1 (P3) gave a better effect on the number of pods, pod weight, seed weight and seed production t ha-1.
Viability and Inhibition of Endophytic Bacteria Pseudomonas fluorescens 4RS1 Against Phytophthora palmivora in Flour Formula Khaeruni, Andi; Hariyani; Hisein, Waode Siti Anima; Satrah, Vit Neru; Wijayanto, Teguh; Sutariati, Gusti Ayu Kade; Taufik, Muhammad
Jurnal Fitopatologi Indonesia Vol. 18 No. 4 (2022): Juli 2022
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.18.4.145-152

Abstract

Viability and Inhibition of Endophytic Bacteria Pseudomonas fluorescens 4RS1 Against Phytophthora palmivora in Flour Formula Pseudomonas fluorescens 4RS1 is an endophytic bacterium that has good inhibition against Phytophthora palmivora, and it has the potential to be developed as a biological agent for black pod rot disease in cocoa. It is necessary to have a formulation that can maintain viability and inhibition and facilitate its use and application, to apply this bacterial agent at the farm level. This study aimed to determine the ability of talc and tapioca flour as the ingredients of formulations that have strong solubility and maintain the viability and inhibition of P. fluorescens 4RS1. The results showed that the use of tapioca flour as the basis for the formulation of P. fluorescens 4RS1 was better than talc because it had high solubility and low precipitation. The addition of nutrients in the form of brown sugar, peptone, and CMC in the tapioca flour formulation was able to maintain the viability and inhibition of P. fluorescens 4RS1 for 12 weeks of storage.
HOME INDUSTRY - PEMANFAATAN BAHAN TANAMAN UNTUK PEMBUATAN SISTEM PENJERNIHAN AIR Wijayanto, Teguh; Rakian, Tresjia C.; Asniah, Asniah; Rahni, Nini Mila; Hisein, Anima; Dedu, La Ode Arfan
Anoa : Jurnal Pengabdian Masyarakat Sosial, Politik, Budaya, Hukum, Ekonomi Vol 5, No 1 (2024):
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52423/anoa.v5i1.48415

Abstract

This Community Service Program has been implemented in Kambu Village, Kambu District, Kendari, Southeast Sulawesi, with the target audience, especially the Housewife Group. Like other areas near the sea coast, problems with the availability of clean water and pollution of water sources often occur, including those often faced by the community in Kambu Village, Kendari. The solution and target offered was to carry out outreach and technical guidance as well as a demonstration of developing a simple water purification system, using natural coagulants from plants or plant residues and other local resources, which can be easily obtained in our environment, including Moringa oleifera seed powder or banana peels, as an alternative source of organic coagulant for absorption, agglomerates and neutralizes the surface tension of mud particles and heavy metals contained in waste substances. This service activity was carried out through method stages: socialization (technical guidance and discussion), demonstration and application of appropriate technology for water purification based on local resources, as well as mentoring. This service activity was carried out by actively empowering the target group through outreach activities, demonstrations and technical guidance. The results of service activities showed: 1) the importance of establishing work programs and implementing science and technology that were in accordance with the needs and conditions that exist in the target community; 2) the implemented work program received a very good response, response and attention from the community and local government officials; 3) the community was greatly helped and gains additional knowledge and skills by implementing appropriate technological innovations; 4) with this service program the relationship between higher education institutions as a source of knowledge with the community and local government improved
Co-Authors Abdul Madiki Aminuddin Mane Kandari, Aminuddin Mane Andi Awaluddin Andi Bahrun Andi Khaeruni ANDI KHAERUNI Andi Khaeruni Andi Khaeruni R, Andi Andi Khaeruni R. ANDI NURMAS, ANDI Anima Hisein ANIMA HISEIN, WAODE SITI Anwar Anwar Arief, Norma Arsana, Made Widana Arsyad, Mirza Arsiaty Asniah Asniah Awaluddin, Andi AZHAR ANSI, AZHAR Azhar Bafadal Azhar Bafadal Bambang Supriadi Benardi Botek, Muhammad Cahyani, Imas Nur Candra Ginting Darmawansyah Darmawansyah Dedu, La Ode Arfan DIRVAMENA BOER, DIRVAMENA Eko Aprianto Johan Ente, La Erwin Najamuddin Etikawati, Made Febrianti Gusnawaty Gusnawaty Gusti Ayu Kade Sutariati Gusti R. Sadimantara Hadi Sudarmo Hairil Adzulyatno Hadini Hariaty Hasan, Rachmi Hariyani Hijria, Hijria Hisein, Anima Hisein, Waode Siti Anima Kadek Sutariati, Gusti Ayu Kilowasid, Muhammad Harjoni La Ode Safuan La Ode Santiaji, La Ode Lailatul Nuraini Laode Sabaruddin Makmur Jaya Arma Mangalla, Lukas K. MARIADI MARIADI Mila Rahni, Nini Muhammad Aldi, Muhammad MUHAMMAD TAUFIK Muhidin Muhidin Musa, Musril Hakim Nasution, Annio Indah Lestari NELI SUSANTI, NELI Ni Wayan Sri Suliartini Ningsi Umar, Marnia Nini Mila Rahni NORMA ARIF, NORMA Nur Aida, Nur NUR ALAMSYAH, MUHAMAD Nuraida, Wa Ode Nuraida, Waode Nurul Amalia Putri, Novita Pramahsari Rachmawati Hasid Rakian, Tresjia C. Sadimantara, Gusti Ray Samirin, Samirin Satrah, Vit Neru Satrah, Vit Neru Sitti Aida A. Taridala Suaib SUDIARSIH, TIRA Suliartini, Ni Wayan Sri Surni Surni Syair Syair Syair, H Syamsiar, Syamsiar SYAMSU ALAM Tanza Nirmala Vit Neru Satrah WA ODE AFU, WA ODE Waode Siti Anima Hisein Yani, Wa Ode Rahzia Zohorman Zohorman Zohorman Zohorman