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All Journal HAYATI Journal of Biosciences Maksimum : Media Akuntansi Universitas Muhammadiyah Semarang Folia Medica Indonesiana Jurnal Matematika dan Ilmu Pengetahuan Alam Prosiding Seminar Biologi Journal of Tropical Biodiversity and Biotechnology Health Notions SAR (Soedirman Accounting Review): Journal of Accounting and Business Biosaintifika: Journal of Biology & Biology Education J-MAS (Jurnal Manajemen dan Sains) SEIKO : Journal of Management & Business Martabe : Jurnal Pengabdian Kepada Masyarakat Jurnal Vokasi Indonesia NISBAH: JURNAL PERBANKAN SYARIAH OIKOS: Jurnal Kajian Pendidikan Ekonomi dan Ilmu Ekonomi Jurnal Respirologi Indonesia Darmabakti Cendekia: Journal of Community Service and Engagements Abdi: Jurnal Pengabdian dan Pemberdayaan Masyarakat Jurnal Riset Biologi dan Aplikasinya Jurnal Pengabdian kepada Masyarakat Nusantara EKOMABIS: Jurnal Ekonomi Manajemen Bisnis JPM: JURNAL PENGABDIAN MASYARAKAT Berkala Penelitian Hayati JURNAL INOVASI DAN PENGABDIAN MASYARAKAT INDONESIA Journal of Accounting and Finance Management (JAFM) Prosiding University Research Colloquium Safari : Jurnal Pengabdian Masyarakat Indonesia Jurnal Ilmiah MEA (Manajemen, Ekonomi, dan Akuntansi) Jurnal Terapan Ekonomi dan Bisnis (JTEB) Jurnal Vokasi Indonesia Riset Akuntansi dan Keuangan Indonesia Sosial Simbiosis: Jurnal Integrasi Ilmu Sosial dan Politik E-Jurnal Akuntansi

Dwi Winarni

Department Of Biology, Faculty Of Science And Technology, Universitas Airlangga

Author-ID : 418198

Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Public Health Social Sciences Transportation Other

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Activity test of various mangosteen (Garcinia mangostana) pericarp extract fractions to decrease fasting blood cholesterol levels and lipid peroxidation activity in diabetic mice Saikhu Akhmad Husen; Dwi Winarni; Firas Khaleyla; Septian Hary Kalqutny
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 22 No 1 (2016): December 2016
Publisher : The East Java Biological Society

The objectives of this study were to determine the effect of various fractions of mangosteen (Garcinia mangostana) pericarp extract to the changes of the fasting blood cholesterol and serum malondialdehyde (MDA) levels on diabetic mice (Mus musculus). Thirty 3-4 months old male mice strain BALB/c, weight 20-30 g were divided into six groups. The first group was KN as a non diabetic control group, KD as a diabetic control, KM as a group of diabetic mice treated with metformin, and NP, SP, and P as the treatment groups that were treated by using three different fractions from mangosteen pericarp extract, non polar, semi polar, and polar respectively. The induction of Diabetes mellitus was done by the injection of STZ, and the mice were given a high fat diet treatment to induce the hiperlipidemia condition using lard for three weeks. The blood cholesterol levels were measured in all groups before and after the injection of lard, and day 1, 7, and 14 of treatment as well. The serum MDA level as the indicator of lipid peroxidation were measured by using QuantiChrom TBARS Assay Kit (DTBA-100). The data of cholesterol levels were statistically analyzed by t-test, while the data of serum MDA levels were analyzed by variance analysis followed by Duncan test. The results showed that the polar fraction of mangosteen pericarp had effect to decrease the fasting blood cholesterol level in mice, however the non polar and semi polar fraction had no simmilar effect. All of the fractions has shown significant effect to decrease the serum MDA level in mice.

AKTIVITAS POLISAKARIDA KRESTIN DARI EKSTRAK Coriolus versicolor TERHADAP PENINGKATAN ANTIBODI Mus musculus AKIBAT PAPARAN Mycobacterium tuberculosis Sri Puji Astuti Wahyuningsih; Amalia Nur Mashita; Dwi Winarni
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 17 No 2 (2012): June 2012
Publisher : The East Java Biological Society

This research aimed to know the bioactivity of polysaccharide krestin (PSK) with a different timing on formation of mice antibody exposed by Mycobacterium tuberculosis. Mycobacterium tuberculosis was used as infection agent that had an abilitiy to be imunogenic and to lead immune system responses decreased. Polysaccharides krestin was used as immunopotentiator which had a role to activate macrophage and stimulate B cell in order to produce antibody. This research involved 30 adult female mice of Mus musculus strain Balb/C in the age of 8–10 weeks and in the weight between of 30–40 g. Mice were divided into six groups, as follows: group K as a control, was added only aquades; group K+ as positive control, was added only PSK; group K- as negative control, was exposed by M. tuberculosis only; group P1 was added PSK before being exposed by M. tuberculosis; group P2 was added PSK after being exposed by M. tuberculosis; group P3 was added PSK before and after being exposed by M. tuberculosis. Polysaccharides krestin was added by gavage with single dose of 500 μg and M. tuberculosis was exposed intraperitoneally with concentration of 5x108 bactery per ml. The bioactivity of PSK was observed by formation antibody using indirect ELISA test. The results of research were the average of OD value of K group was 0,590 ± 0,042, K+ group was 0.641 ± 0.025, K- group was 1.044 ± 0.054, P1 group was 1.032 ± 0.125, P2 group was 1.127 ± 0.042, P3 group was 1.230 ± 0.097. The conclusion of this research showed that the adding of PSK increased mice formation antibody. The time of adding before and after being exposed M. tuberculosis was the most potential to raise antibody production on mice.

EFEK EKSTRAK AKAR GINSENG JAWA DAN KOREA TERHADAP LIBIDO MENCIT JANTAN PADA PRAKONDISI TESTOSTERON RENDAH Dwi Winarni
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 12 No 2 (2007): June 2007
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/347

This research was designed to compare the potency and duration effect of Java ginseng and Korean ginseng root extract administration on sexual behavior of male mice. It was done experimentally on male mice (strain BALB, aged 8–10 weeks, weighed 25–35 g). Thirty eight mice were grouped to 4 (four) groups: First group was treated with solvent (as positive control), 2nd group was treated ethynilestradiol (EE2) (as negative control), 3rd group was treated with Java ginseng root extract, and 4th group was treated with Korean ginseng root extract. All groups were administered with EE2 0.56 mg/20 g bw/day for 9 days as pretreatment to decrease the testosteron level. After pretreatment, each group divided to 3 subgroups (each would receive treatment for 9, 18, and 27 days). Ethynilestradiol 0.56 mg/20 g bw/day was administered along treatment to keep testosterone level low, except to positive control group. Java ginseng and Korean ginseng root extract (equal with 1.4 mg ginseng root powder/ 20 g bw/day) and EE2 were administered orally. The level of testosterone after pretreatment was measured by RIA (radioimmuoassay) and changes in libido were determined by libido test. After the last treatment, 1 male mouse kept singly in individual cage. Libido test was carried out for 20 minutes. All of these activities during test recorded by handycam. The mice were observed for time from the introduction of female into the cage of male upto the first mount (mounting latency/ ML) and for the number of mounts (mounting frequency/MF). The results indicated that at low testosterone level, Java ginseng root extract administration at the dose equal with 1.4 mg ginseng root powder/20 g bw/day shortened mounting latency and increased mounting frequency but Korean ginseng extract root at the same dose gave negative effects. Duration of administration of both Java and Korean ginseng root extract didn’t give effect on libido.

EFEK 2-METHOXYETHANOL TERHADAP STRUKTUR HISTOLOGI TESTIS MENCIT (Mus musculus) Alifah Hayati; Binti Yunaida; I.B. Rai Pidada; Win Darmanto; Dwi Winarni
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 10 No 1 (2004): December 2004
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/371

This research has done to investigate the effect of 2-Methoxyethanol on the testicular histology of the male mice and also the influence the length of time after administration 2-ME stopped in the recovery of the spermatogenic cells and the diameter also the thicknes of seminiferous tubule. Thirty BALB/C male mice 8–9 week old, weighed 28–30 grams body weight. Those mice separated to 6 groups with 5 male mice each group. Those mice were treated with 2-ME 200 mg/kg body weight daily by intra peritoneal injection, within 3 weeks (K1). To investigate the influence the length of time after administration 2-ME stopped, the male mice after treated by 2-ME in 3 weeks also given by the length of time after 2-ME administration stopped 1, 2, 3 and 4 weeks (P1, P2, P3 and P4). The control animal given by intraperitoneal administration of saline. Histological observation was performed on the number of spermatogonium, primary spermatocyte, oval spermatid and the diameter also epithelial thickness of seminiferous tubules. The data were analyzed by One-Sample T-test to investigate the differences between K0 and K1. One Way ANOVA to investigate the influence the length of time after 2-ME administration stopped in the P1, P2, P3 and P4 and then continuing by LSD (Least Significant Difference) to show the differences groups of treatment. The result showed that administration 2-ME could destroy the seminiferous tubules in the testes. Its presented by the decreasing of the number spermatogonium, primary spermatocyte, oval spermatid and diameter also epithelial thickness of seminiferous tubule. The length of time after administration 2-ME stopped could recover seminiferous tubules condition. Its presented by the increasing of the number spermatogonium, primary spermatocyte, oval spermatid, and diameter also epithelial tickness of seminiferous tubules. The conclution of this research were, 2-ME could destroy the testicular histology of the male mice and the length of time after administration 2-ME stopped have linear correlation with seminiferous tubules recovery.

PENGARUH PEMBERIAN KADMIUM TERHADAP TINGKAT KELANGSUNGAN HIDUP DAN KERUSAKAN STRUKTUR INSANG DAN HEPATOPANKREAS PADA UDANG REGANG [Macrobrachium sintangense (de Man)] Agoes Soegianto; Nia Adiani Primarastri; Dwi Winarni
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 10 No 1 (2004): December 2004
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/421

The purpose of this research was to find out the impact of cadmium on the structure of gills, hepatopancreas and on survival of shrimp. The shrimp was divided into four groups. Group I (control) was exposed to 0 ppb of cadmium, group II exposed to10 ppb of Cd, group III exposed to 20 ppb of Cd and group IV exposed to 30 ppb of Cd. Three replications were applied in each treatment. Survival rate data were collected everyday, and every two days the water was substituted with new water. The experiment was stoped when 50% of shrimps dead. The rest of shrimps were prepared for histological study. All data were subjected on Kolmogorov-Smirnov (distribution test) then continued with ANOVA test and Kruscal-Wallis tests. The result of this experiment showed that structural damage on gills and hepatopancreas increase with increasing cadmium concentration in medium. The lamella of shrimp from control group did not show hyperplasia and necrosis; in second group: 24.02% of lamella showed hyperplasia, 2.77% necrosis and 28.02% vacuolization; third group: 70.01% of lamella showed hyperplasia, 20.60% necrosis and 48.79% vacuolization; fourth group: 32.60% of lamella demonstrated hyperplasia, 57.35% necrosis and 97.50% vacuolization. Increasing the structural damage of gills and hepatopancreas, it cause the decrease on survival rate of shrimp.

PERSISTENSI TOKSISITAS BIOINSEKTISIDA SPHEREFIXTM PADA BEBERAPA TIPE TEMPAT PERINDUKAN NYAMUK Aedesaegypti L. Salamun Salamun; S A Husein; Rosmanida Rosmanida; Dwi Winarni; Tri Nurhariyati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 6 No 1 (2000): December 2000
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/480

SpherefixTM bioinsecticide is microbial agent of Bacillus sphaericus H-5a5b (VCRC B24) showing high potency for vector control. The bioinsecticide specific to target insect, and do not produce any adverse environmental impact, so the bioinsecticide would be very promising agent for vector control, especially vector of dengue haemorrhagic fever in Indonesia. The present studies aimed at observing the toxicity persistence of SpherefixTM on the larvae Aedesaegypti L. in some types of water container, and recycling potencies in the breeding places of A.aegypti. Two steps of the studies were carried out under laboratory conditions. Firststeps were reared mosquitos in the laboratory to supply larvae of Aedes aegypti. Second step testing of SpherefixTM toxicity persistence in the cemented, clay, and plastic containiers. The bioinsecticides were preparated by Vector Control Research Centre (VCRC), India. The toxicity persistence of SpherefixTM on the larvae of A. aegypti L. in some types of water container were decided by probit analysis. The toxicity persistence tests were carried out by time series observation on the day 1, 6, 12,,,, and 120. The result showed that difference toxicity persistence of the SpherefixTM in the breeding places of A. aegypti. The cemented container was found to have longest of toxicity persistence of the SpherefixTM followed by the plastic container, and the clay container ha the shortest duration toxicity persistence. The higher concentration of the boinsecticides, will result in higher toxicity persistence. The SpherefixTM showed recycling potency in the breeding places of A. aegypti. Percent motility of the larvae dropped sharply after day 50, and larvae mortality under 5% after day 78.

PENGARUH EKSTRAK AKAR GINGSENG JAWA DALAM BERBAGAI KADAR TERHADAP SINTESIS PROTEIN SERUM TIKUS Tri Nurhariyati; Dwi Winarni; Salamun Salamun; Y Sri Wulan Manuhara; Saikhu Ahmad Husen
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 6 No 1 (2000): December 2000
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/482

The aim of this research was identifying the stimulation serum protein synthesis following the treatment of Java Gingseng (Talinum paniculatum Gaertn) root extract in varying concentration. This research used 12 male rats of 8-9 weeks, divided into 4 groups. First group as control, second group to five group were administratered with Java gingseng root extract equal with 3.5, 10.5, and 21 mg dry roots / 100g BW / day per oral for 14 days. Blood serum was collected for electrophoresized at the end of treatment. Electrophoresis was conducted with 7% SDS-PAGE method. Gel stained in 0.25% Coomasie Brilliant Blue and it followed densitometry on band in gel to comparing those quantitatively. The result show the effect on the level of serum protein whose MW 29. Highest stimulation of protein synthesis (2.2 times than control) have been reached at treatment with extract that equal with 3,5 mg dry root/ 100 g BW/ day. Increasing Java gingseng root extract wasnâ€™t followed by increasing of stimulation protein synthesis level.

PENGARUH EKSTRAK AKAR GINGSENG JAWA DAN GINGSENG KOREA TERHADAP SINTESIS PROTEIN TESTIS YANG BERPERAN DALAM SPERMATOGENESIS Dwi Winarni
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 5 No 1 (1999): December 1999
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/524

This study was aimed to know the effect of the Jawa ginseng and Korean ginseng roots on synthesis of testicular proteins that needed for spermatogenesis. Eighteen male rats (8-9 weeks) were divided into 4 groups. Rats were given orally with ethinylestradiol (6 rats-group I), ethinylestradiol and extract of Korean ginseng root (3 rats-group II), ethinylestradiol and extract of Java ginseng (3 rats-group III), and drinking water only (6 rats-group IV). Ethinylestradiol that administrated equal to 1 mg/100 g body weight/day for each rat but extract of Java ginseng or Korean ginseng roots was equal with 3.5 mg dry ginseng root / 100 BW/each day. That treatment was done for 14 days. The testes was collected on the last day of treatment. Testes from three rats each from group I and group IV were sectioned by paraffin method and stain with hematoxylin-eosin but other testes (3 rats for each group) were made to homogenate. Testicular extract was made from I testicular homogenate : 3 phosphate buffer saline. Testicular proteins were separated by 10% SDS-PAGE. Gel was stained with Coomasie brilliant blue 0.25%. According to protein bands in gel and histological observation, this study revealed that the administration of extract of both Java ginseng and Korean ginseng roots equal with 3.5 mg dry ginseng root/ 100 BW/ day for 14 days, influence synthesis of testicular protein that have role on meiotic stage of spermatogenesis. These proteins have MW 14.44 and 68 kDa.

The Comparison Effect of Electrical Cigarrete and Conventional Cigarrete Smoke toward White Rat’s (Rattus norvegicus) Lung Histopathology Yoan Asri Triantara; Inggit Almira; Sarwan Adi Kusumo; Muhammad Fajar; Dicky Darmawan; Dwi Winarni
Jurnal Respirologi Indonesia Vol 39, No 2 (2019)
Publisher : Perhimpunan Dokter Paru Indonesia (PDPI)/The Indonesian Society of Respirology (ISR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36497/jri.v39i2.52

Background: The number of cigarettes smoked per day has an average of 12,3% of cigarettes in 2013. The increase of the conventional cigarettes price in 2016 made some conventional smokers switch to electrical cigarettes because more safety than conventional One of the indicators of pulmonary damage is the increase of alveolar macrophage that cause pulmonary structural change triggered by substances in the cigarette. Methods: Thirty two of white rat were divided into four groups (control, conventional cigarette, electrical cigarette with 0 miligram (mg) nicotine, and electrical cigarette with 3 mg nicotine) and were exposed for 35 days (30x2 minutes; in the morning and evening). Results: The group exposed to conventional cigarette smoke has the highest average number of macrophages than the cigarette smoke group with nicotin levels of 0 mg, electric cigarette with 3 mg nicotine and control. Brown Forsythe test continued with Games-Howell post hoc test indicates that there are significant mean difference between each group except between group exposed to electric cigarette smoke with 3 mg nicotine and exposed to conventional cigarette smoke. Based on the indicator assumption of lymphoid infiltration and thickening and fusion of the alveolar, the smoke of conventional cigarette and electric cigarette with 3 mg nicotine caused more histopathological damage to the white rat’s lungs than of conventional cigarette than the smoke of electric cigarette with 0 mg nicotine and those in the control group. Conclusion: The exposure of conventional cigarette smoke caused the highest damage to Rattus norvegicus’ pulmo according to macrophage alveolar and histopathological indicator, but not significantly different with the exposure of electrical cigarette with 3 mg nicotine. The exposure of electrical cigarette with 0 mg nicotine caused minor damage equals to control group based on histopathological indicator. (J Respir Indo 2019; 39(2): 88-91)

Finding the Optimized Frequency of Electric Field on The Attempt of Reducing Blood Sugar Level in Type II Diabetes Patients Suhariningsih Suhariningsih; Dwi Winarni; Saikhu Akhmad Husen; Tri Anggono Priyo
Jurnal Vokasi Indonesia Vol 6, No 1 (2018): January - June
Publisher : Program Pendidikan Vokasi Universitas Indonesia

A study has been conducted to test the use of electric fields to lower blood sugar levels on rats with type II Diabetes Mellitus. This study uses male rats (Rattus novergicus), aged 8-12 weeks weighing 150-200 grams, with a total of 31 rats. Therapy was conducted by putting rats in a chamber that emits an electric field every day for 1 hour for 28 days. To search for the optimum frequency of the treatment, frequency variations of 15, 30, 60, and 120 kHz were given. The electrical charge distribution of the air in the room was then measured, whereas mice blood sugar levels were also measured to reveal the effect of the treatment on cell tolerance to sugar, and to come up with the measurement of insulin levels at the end of the treatment. The results of measurements of the electrical charges in the air were very significant generally showing that the treatment with a frequency of 15 to 120 KHz for 4 weeks in the experimental animals could lower their blood sugar (fasting period), but the success rate significantly occurred at a frequency of 15 kHz. The decrease in fasting blood sugar levels after the treatment was probably due to the influence of the treatment (the influence of an electric field) that improved the regulation of insulin receptors. Improvements in the regulation of insulin receptor activities with high gluconeogenesis may be the cause of a decrease in fasting blood sugar levels that is not accompanied by improved cell tolerance to sugar. Keywords: Electric Field, blood sugar levels, insulin levels

Co-Authors Abdul Hakim Muhsyi Adhisti, Raya Anandita Puspaning Adlina, Rediarta Nisa Agoes Soegianto Agus Supriyanto Ahimsa Buena Merpati Alfiah Hayati Alifah Hayati Almando Geraldi Amalia Nur Mashita Amany, Ashilla Nadiya Amir Amir Ani Kusbandiyah Ani Kusbandiyah Anjar Tri Wibowo Annisa, Rully Aprilia Nurcahyaning Rahayu Arofah, Rifka Utami Arwati, Niluh Arwati, Niluh Ayu Setyaningrum Bambang Wirjatmadi Binti Yunaida Boon Chin Tan Defi Nurul Hayati Dewandaru, Rhis Ogie Dicky Darmawan Dirgantari, Novi Eko Hariyanto Erillia, Titan Erlyn Nurul Fauziah fatimah Fatimah Firdaus Auliya, Zahra Fitriati, Azmi Gilang Permana Hadi Pramono Hidayati, Farisa Nabila Hijrianti, Zora Lutvya Sabrina Husen, Saikhu Akhmad I.B. Rai Pidada Idqa Nurtri Bhakti Inggit Almira Inne Ninda Resita Intan Permata Putri Intan Permata Putri ISTUNING MA’UNAH Iwan Fakhruddin Iwan Fakhruddin Junairiah Junairiah Khaleyla, Firas Khoerul Mukromiin, Ramadhan Lestari, Lita I.D. Listijani Suhargo, Listijani M., Soenarnatalina Manikya Pramudya Marta Tania Gabriel Ching Cing, Marta Tania Gabriel Merryana Adriani Mochammad Affandi Muhammad Fajar Nabilatun Nisa Nabilla, Nithasya Nadya Abanty, Fahma Nadyatul Ilma Indah Savira Nazah, Nawalin Neti Mustikawati Ni Wayan Sukma Antari Nia Adiani Primarastri Nimatuzahroh, Nimatuzahroh Norwani, Norlia Mat Nurmajid, Difa Naufal Pandansari, Tiara Pratama, Bima Cinintya Priyo, Tri Anggono Rahayu, Aprilia Nurcahyaning Rosmanida S A Husein Said Akmala Saikhu Ahmad Husen Saikhu Akhmad Husen Salamun Salamun Salamun Salamun Salsabila, Syarifah Sarwan Adi Kusumo Septian Hary Kalqutny Sifa, Mufatih Ariska Sifananda Soenarnatalina M. Soenarnatalina Meilanani Soenarnatalina Meilanani, Soenarnatalina Sri Hartiningsih Sri Hartiningsih Sri Hartiningsih Sri Puji Astuti Wahyuningsih, Sri Puji Astuti Sri Wahyuni Sri Wulan Manuhara, Yosephine Sugiarso, R. Djarot Sugiharto Sugiharto Suhariningsih Titan Erillia Tri Anggono Priyo Tri Nurhariyati Triana Safitri, Nadia Ufairanisa Islamatasya Ufairanisa Islamatasya Wijatmadi, Bambang Win Darmanto Y Sri Wulan Manuhara Yoan Asri Triantara Yosephine Sri Wulan Manuhara Zaidatul Khauliyah, Desi Zamakhsyari, Luthfi

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