Article Per Year (5 Year)
p-Index From 2021 - 2026
3.057
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences SAINS MEDIKA : JURNAL KEDOKTERAN DAN KESEHATAN Jurnal Ilmiah Aplikasi Isotop Radiasi Microbiology Indonesia Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Journal of Biomedicine and Translational Research The Indonesian Biomedical Journal Biomedical Journal of Indonesia Universa Medicina Indonesian Journal of Biotechnology and Biodiversity The Indonesian Journal of Infectious Diseases Eksakta : Berkala Ilmiah Bidang MIPA Majalah Kedokteran Andalas Health Information : Jurnal Penelitian Jurnal Ners East Asian Journal of Multidisciplinary Research (EAJMR) Journal of Comprehensive Science Makara Journal of Health Research Makara Journal of Science Journal of Clinical Microbiology and Infectious Diseases Indonesian Journal of Biomedicine and Clinical Sciences
Andi Yasmon
Department Of Microbiology, Faculty Of Medicine Universitas Indonesia, Cipto Mangunkusumo Hospital, Jakarta
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Neuroscience Nursing Public Health Other

Published : 31 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 31 Documents
Search

 1   2   3   4 
Optimization of Duplex Real-time PCR to Detect Corynebacterium ulcerans and C. pseudotuberculosis from Patients with Suspected Diphtheria in Jakarta Usman, Doni; Rosana, Yeva; Yasmon, Andi
Biomedical Journal of Indonesia Vol. 12 No. 2 (2026): Vol 12, No 2, 2026, In Press
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bji.v12i2.319

Abstract

Introduction. Diphtheria infection remains a serious public health problem in many countries with low vaccine coverage. Molecular detection plays an important role in controlling disease dissemination. Among more than 122 Corynebacterium species, only three are potentially toxigenic: C. diphtheriae, C. ulcerans, and C. pseudotuberculosis. Infections caused by C. ulcerans and C. pseudotuberculosis in humans usually involve close animal contact. Due to the many difficulties in identifying C. ulcerans and C. pseudotuberculosis, this study aimed to optimize Duplex Real-time PCR for rapid and precise detection. Method. Duplex real-time PCR was performed by using 2 pairs of primers and a probe to detect C. ulcerans and C. pseudotuberculosis. All parameters were optimized to maximize PCR amplification. Then, the primer set's specificity was tested against some microorganisms. The sensitivity test of DNA was conducted to get the detection limit. Results: Optimization of real-time PCR was conducted to achieve optimal amplification conditions.  Annealing temperature 57 °C, Optimization of primer and probe concentration of 0.45 μM and 0.50 μM for C. ulcerans, Primer and probe concentrations of 0.35 μM for C. pseudotuberculosis yielded optimal results. The detection limit of DNA of duplex real-time PCR for C. ulcerans was 4.49 DNA copy number and 1.06 DNA copy number for C. pseudotuberculosis. Conclusion. Duplex Real-time PCR optimization result can be used as a detection of C. ulcerans and C. pseudotuberculosis for effective control of dissemination in the population. PCR results showed that all 108 clinical specimens suspect diphtheria were negative for C. ulcerans and C. pseudotuberculosis.
Co-Authors . Andriansjah Abror Irsan Ade P.R. Simaremare Agustini, Riani Anis Anis Anis Karuniawati Ardiana Kusumaningrum Ariyani Kiranasari ASRI SULFIANTI Beti Ernawati Dewi Budiman Bela Burhanuddin Burhanuddin Burhanuddin Burhanuddin Delly Chipta Lestari Delly Chipta Lestari Dewi Murniati Diah Dwi Utami Diana Natalia Diana Natalia Efendi, Ida Effendi, Ida Effiana Effiana Effiana, Effiana Elisabeth D. Harahap Elisna Syahruddin Erlina, Linda Fadilah Fadilah Fadilah FERA IBIRAHIM Fera Ibrahim Fithriyah Fithriyah Fithriyah Fithriyah Fithriyah Fithriyah Gultom, Desy A. Heri Wibowo Ibnu Agus Ariyanto Ika Ningsih Janah, Dariatul Junita Indarti Ketut Tuti Parwati Linosefa Linosefa Lisnawati Rachmadi Lola Febriana Dewi Louisa Ivana Utami Lucky H Moehario Lucky Hartati Moehario Mardhia, Mardhia Mardhia, Mardhia Mardiastuti H Wahid Maria L. Rosilawati Maria L. Rosilawati Maria Lina R. Maria Lina Rosilawati Maya Ulfah Murti, Anissa Wari Ni Made Adi Tarini Ni Nengah Dwi Fatmawati Normasari Normasari Oktania Sandra Puspita Paramita, Rafika Indah Pramita G. Dwipoerwantoro Pratiwi Pujilestari Sudarmono Purba, Hastuti Handayani S Purnomosidi, Muhammad Abhi Rahayu, Ratih Ratnoglik, Suratno Lulut Rela Febriani Ria Kodariah Rifdah Hanifah Rosa, Yulia Rudyanto Sedono Safari, Dodi Salsabila, Korrie Samsuridjal Djauzi Sari Rahmayanti Simon Yosonegoro Liem SJATHA, FITHRIYAH Sudarmo, Fitrahwati Tafroji, Wisnu Teguh Sarry Hartono Thomas Robertus Tjampakasari, Conny Riana Usman, Doni Utami, Diah Dwi VIVI SETIAWATY Winarti, Yayah Wresti Indriatmi B. Makes Wulandari, I Gusti Ayu Inten Yeva Rosana Yulia Rosa Saharman YULIANTY MUHAYAR Yusmaniar Yusmaniar Zaenab