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Journal : Makara Journal of Science

Sequence Analysis of the Cytochrome C Oxidase Subunit I Gene of Pseudagrion pilidorsum (Odonata: Coenagrionidae) Tallei, Trina Ekawati; Koneri, Roni; Kolondam, Beivy Jonathan
Makara Journal of Science Vol. 21, No. 1
Publisher : UI Scholars Hub

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Abstract

Pseudagrion pilidorsumis1of over 140 species of Pseudagrion (in thefamily Coenagrionidae), the largest genus of damselfly. This species exhibits dimorphism due to the different body colorations of males and females, making them difficult to distinguish from other congeneric species. This study analyzed thecytochrome C oxidase subunit I (COI) gene sequence of P.pilidorsum found in Bogani Nani Wartabone National Park (North Sulawesi) and compared it with other sequences of P. pilidorsum from distinct geographical locations in Asia. The COI gene for the Sulawesi specimen was amplified using the universal primer pair LCO1490 and HCO2198. A sequence homology search was conducted through BLAST. Multiple sequence alignment was executed using CLUSTALO (1.2.1). A phylogenetic tree was constructed using the Neighbor-Joining method, and genetic distance was calculated using the Kimura 2-parameter. The COI gene sequence of the Sulawesi specimen lies in the range of 83.99-89.10% with other P. pilidorsum deposited at Gen Bank, namely KF369526 (Sarawakspecimen), AB708543, AB708544, and AB708545 (Japan specimens). The genetic distance falls in the range of 0.146-0.149 between the Sarawak specimen and the Japan specimen; 0.122-0.125 between the Sulawesi and Japan specimens; and 0.185 between the Sulawesi specimen and the Sarawak specimen. It can thus be inferred that the Sarawak and Japan specimens may not belong to the same species; the Sulawesi and Japan specimens may not belong to the same species; and the Sarawak specimen and Sulawesi specimens might be placed in different genera.
Co-Authors Abas, Abdul Hawil Adikila, Gregorius Giani Angelina Stevany Regina Masengi Antasionasti, Irma Any Aryani Arifin, Mulyani Asep Rusyana Azzania Fibriani Balansa, Endrile Golmen Barasarathi , Jayanthi BEIVY JONATHAN KOLONDAM Celik, Ismail Daniel Febrian Sengkey Dantje Tarore Diah - Kusumawaty Diah Puspitasari Dian Handayani Diana Setya Ningsih, Diana Didik Priyandoko Dolongtelide, Jeclin Inebel Dzikrina, Hanina El-Shazly, Mohamed Elly Suoth Emran, Talha Bin Erwin Wantasen Estevam, Ethiene Castellucci Faisal, Farassa Rani Fatimawali . Florencia N. Sompie Ghazi Mauer Idroes Halimatushadyah, Ernie Hariyanto, Yuanita Amalia Herny E.I. Simbala Hizir Sofyan Idroes, Ghifari Maulana Illah Sailah Irvanizam, Irvanizam Jein Rinny Leke, Jein Rinny Kalalo, Marko Jeremia Kemala, Pati Kepel, Billy Johnson Khairan Khairan Laksono Trisnantoro Lala, Andi Lydia E. N. Tendean, Lydia E. N. Mamahit, Juliet Merry Eva Martha Marie Kaseke Masengi, Kyoko Itsuko Etsuko Gabriela Maulana, Aga Maulydia, Nur Balqis Mirda, Erisna Moh. Yani Mohd Fauzi, Fazlin Monoarfa, Alexander James Muliadi Ramli Musdalifah, Annisa Nabila, Fiki Farah Niode, Nurdjannah Jane Nurul Faridah, Nurul Patwekar, Mohsina Paulina yamlean Pendong, Christa Hana Angle Purukan, Christy Purwanto, Diana Shintawati Rahman, Sunarti Abd Ratte, Titah Amelia Rinaldi Idroes Rizkia, Tatsa Roni Koneri Runtunuwu, Stefanus Vicky Bernhard Elisa Salaki, Christina Leta Salaswati, Salaswati Sambul, Alwin Melkie Sari, Nadia Warda Sekar Sasmita, Novi Reandy Siampa, Jainer Pasca Sri Sudewi, Sri Takawaian, Agrita Feisilia Tamala, Yulianida Tania, Adinda Dwi Tendean, Lydia Estelina Naomi Teuku Rizky Noviandy Tumilaar, Sefren Geiner Turalaki, Grace Lendawati Amelia Unsratdianto Sompie, Sherwin Reinaldo Utami, Wulandari Putri Wawo, Arsianita Ester Wijaya, Puspita Wungouw, Herlina Ineke Surjane Zuchra Helwani, Zuchra Zulkarnain Jalil