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All Journal Jurnal Akuakultur Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Pembangunan Pedesaan Jurnal Biologi Tropis Biosaintifika: Journal of Biology & Biology Education Jurnal Riset Akuakultur Jurnal Pengabdian Magister Pendidikan IPA Aquacultura Indonesiana Journal of Aquaculture and Fish Health MARLIN : Marine and Fisheries Science Technology Journal Jurnal Pengabdian kepada Masyarakat Nusantara Jurnal MAIYAH Molekul: Jurnal Ilmiah Kimia Jurnal Perikanan Journal of Artha Biological Engineering

Purnama Sukardi

Fakultas Perikanan dan Ilmu Kelautan Universitas Jenderal Soedirman

Author-ID : 436594

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Veterinary Other

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Evaluasi Potensi Tepung Tubifex sebagai Pengganti Tepung Ikan dalam Pakan Benih Ikan Nila (Oreochromis niloticus) Yusup, Dandi Diana; Simangunsong, Tohap; Sukardi, Purnama
Jurnal Perikanan Unram Vol 15 No 4 (2025): JURNAL PERIKANAN
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jp.v15i4.1730

The high cost and limited availability of fishmeal in aquaculture feed formulations have prompted the search for sustainable and affordable alternative protein sources. This study aimed to evaluate the effects of substituting fishmeal with silk worm (Tubifex sp.) meal on the growth performance, feed utilization, and survival rate of Nile tilapia (Oreochromis niloticus) fry. A completely randomized design (CRD) was used with four dietary treatments: P1 (control, 0% Tubifex), P2 (15%), P3 (25%), and P4 (35%), each with three replications. Fish were fed twice daily for 30 days, and growth parameters were measured weekly. Results showed that the inclusion of Tubifex meal significantly improved the final weight, specific growth rate (SGR), average daily gain (ADG), and feed efficiency (FE), particularly in P3 and P4. The highest SGR (2.28%/day), ADG (0.016 g/day), and FE (61.93%) were recorded in the P4 group. Survival rate also improved, reaching 82.22% in P4 compared to 51.11% in the control. Water quality parameters remained within optimal ranges, indicating no adverse environmental impact from Tubifex inclusion. The findings suggest that Tubifex meal is a viable protein source that can replace fishmeal by up to 35% in tilapia fry diets. Its application offers nutritional, economic, and ecological benefits, making it a promising component in sustainable aquafeed development.

The Effect of Using an Autofeeder on Vannamei Shrimp (Litopenaeus vannamei) Growth in Intensive Ponds at Cv. Cemara Sewu Sumber Rejeki, Cilacap -, Andreas Eka Saputra; kasprijo, Kasprijo; Sukardi, Purnama
Journal Of Artha Biological Engineering Vol. 2 No. 1 (2024)
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/6jvxk275

The use of automatic feeding equipment can increase the productivity of vannamei shrimp cultivation and harvest yields. This research aimed to determine which of the three feeding methods (manual, 120° autofeeder, and 360° autofeeder) produces greater growth and production results for vannamei shrimp. The shrimp used came from Sura Tani Pemuka which were stocked from 23-25 July 2022 at PL (post larval) age 11. When using a 360° autofeeder with a total area of 5,880 m2 and a density of around 169 individuals/m2. When using a 120° autofeeder with a total area of 2,840 m2 and a density of around 166 fish/m2. The manual method is used with a total area of 2,500 m2 and a density of around 187 individuals/m2. The research method was carried out by means of descriptive observation and analysis. Data collection was carried out during shrimp sampling activities, checking water quality, and harvesting. The research results showed that the use of a 360° autofeeder, 120° autofeeder, and manual resulted in sequential growth performance including: survival rates of 39.37%, 51.47%, and 32.47%; average weight growth of 21.53 gr/head, 17.47 gr/head, and 10.12 gr/head; daily weight growth of 0.31 gr/day, 0.25 gr/day and 0.16 gr/day; and, feed conversion ratios were 1.84, 1.73 and 2.23. The harvest results achieved using the 360° autofeeder, 120° autofeeder, and manual method reached 4558.32 kg, 3670.18 kg, and 1294.965 kg, respectively.

The Effect of Encapsulator Machine Speed on Composite Microcapsule Size Insyira, Athalla Rania; Athari, Hafizha Nur; Sukardi, Purnama; Wijaya, Rudy
Journal Of Artha Biological Engineering Vol. 2 No. 2 (2024)
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/s0btxz58

Microcapsules are small round particles with uniform walls or layers around them that generally have a diameter from 0.2 to 5000 µm. Microencapsulation is a process that converts solid or liquid materials into capsule form which aims to protect and maintain active ingredients from environmental influences such as humidity, high temperature, light exposure, or oxidation processes. Microcapsule formation can be done using the crosslink method. Microcapsule size is affected by the stirring speed of the encapsulator machine. During microencapsulation of fish meal with eggs and gelatin using the crosslink method, the stirring speed is 3000 rpm, 3500 rpm, 4000 rpm, 4500 rpm, and 5000 rpm. In the microencapsulation process of fish meal with eggs and gelatin, the smallest average diameter at a speed of 5000 rpm, which is 18.220 ± 3.3 µm and has a yield of 26% while the largest diameter at a speed of 3000 rpm, which is 33.53 ± 4.8 µm and has a yield of 28.4%. Based on the results of the Tukey SPSS test, the speeds of 3000 rpm (33.53 ± 4.8 µm), 3500 rpm (30.25 ± 4.4 µm), 4000 rpm (26.30 ± 3.5 µm), 4500 rpm (22.50 ± 4.1 µm), and 5000 rpm (18.220 ± 3.3 µm) were very significantly different (P <0.05) in each treatment.

The Effects of Substitution on Fish Meal with Maggot Meal in Artificial Feedfor Eels (Anguilla bicolor) Growth and Survival Rate ahmad, ananda; Sukardi, Purnama; Nugrayani, Dewi; Trianingtyas, Laela
Journal Of Artha Biological Engineering Vol. 3 No. 1 (2025)
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/fwrbjv08

Eel (Anguilla sp.) is a leading freshwater fish commodity. In the intensivesystem, high stocking density results in high production costs. Maggot meal isan alternative raw material that has complete nutritional value such as fishmeal but is cheaper. The purpose of this study was to determine theeffectiveness of substituted fish meal with maggot meal on the growth andsurvival rate (Anguilla bicolor) Elver phase. The method carried out in thisstudy used an experimental method with a completely randomized design(CRD) consisting of 4 treatments and 3 replications with a combination of fishmeal and maggot meal, P0: 100% TI and 0% TM, P1: 80% TI and 20% TM,P2: TI 60% and TM 40% and P3: TI 40% and TM 60%. The results of rearingfor 60 days showed a significant effect on absolute weight growth, specificgrowth rate, and yield, while the yield had no significant effect on absolutelength growth, feed conversion, and survival. The results of the water qualityparameters of the media are temperatures ranging from 24 °C -29,8 °C, pHranging from 5,14-7,7, DO ranging from 2,4 mg/l – 5,1 mg/l and ammoniaranging from 0 ppm – 0,01 ppm.

Effectiveness of Minaraya Probiotics on The Growth Rate and Immune Response of Catfish (Clarias gariepinus) fitriadi, ren; Sukardi, Purnama; Muhammad Riady, reza; nurhafid, mohmammad; Heratri, Agnes; Selia Irawati, Maghfira
Journal Of Artha Biological Engineering Vol. 3 No. 2 (2025): Volume 3 no 2 2025
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/bdxbgh67

The growing demand for catfish in the market is prompting farmers to consistently enhance their production capacity. Several obstacles are encountered during production growth, such as pathogen attacks, suboptimal growth, and low survival rates. This study aimed to determine the effectiveness of Minaraya probiotics with different doses in feed on the growth and immune response of catfish. The method used in this study was an experimental method with a completely randomized design (CRD) consisting of four treatments: control (without probiotics), and treatments P1, P2, and P3 with the addition of Minaraya probiotics at 2, 5, and 10 mL per 2.5 kg of feed, respectively, each with three replications. The parameters observed included survival rate, SGR, DGR, FCR, PER, AF, RB, and leukocyte differentiation. The results showed that the survival rate in all treatments was relatively high, ranging from 95% to 98.89%. The results also showed that the addition of Minaraya probiotics significantly (p<0.05) increased growth parameters (SGR and DGR) and feed efficiency (FCR and PER), with the best dose being in treatment 3 (P3) at a dose of 10 mL/2.5 kg of feed. On the other hand, the non-specific immune response also increased in the treatment given Minaraya probiotics, as indicated by increased phagocytic activity, respiratory burst, and proportion of lymphocytes, neutrophils, and monocytes, as well as an increase in the total number of erythrocytes after Aeromonas hydrophila infection. The application of Minaraya probiotics in a sustainable cultivation system can improve catfish productivity and health.

ISOLASI, IDENTIFIKASI, DAN ANALISIS EKSPRESI GEN PENGKODE GROWTH HORMONE PADA IKAN SIDAT (Anguilla bicolor) Syakuri, Hamdan; Wibowo, Satrio Haryu; Ekasanti, Anandita; Soedibya, Petrus Hary Tjahja; Marnani, Sri; Listiowati, Emyliana; Nugrayani, Dewi; Sukardi, Purnama
Jurnal Riset Akuakultur Vol 19, No 1 (2024): (Maret 2024)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.19.1.2024.69-83

Gen hormon pertumbuhan (growth hormone, GH) perlu dipelajari untuk mendukung domestikasi ikan sidat Anguilla bicolor di Indonesia. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi gen pengkode GH serta menganalisis ekspresinya pada sampel ikan sidat A. bicolor. Primer untuk amplifikasi gen GH ikan sidat A. bicolor didesain berdasarkan sekuen gen GH dari beberapa spesies ikan sidat lain yang tersedia di GenBank. Sampel cDNA hipofisa dan otak ikan sidat digunakan untuk amplifikasi gen GH. Hasil amplifikasi disekuensing dan hasilnya dianalisis menggunakan analisis BLAST, multiple sequences alignment, hormon signature, dan filogenetik. Analisis ekspresi gen GH dilakukan menggunakan teknik realtime PCR dengan metode delta-delta CT pada 14 sampel ikan sidat (23,1 ± 19,6 g; 24,5 ± 4,2 cm). Fragmen DNA sepanjang 486 bp berhasil diamplifikasi dan disekuensing. Sekuen gen GH A. bicolor memiliki similaritas nukleotida sebesar 98,49-99,14% jika dibandingkan dengan gen GH ikan sidat A. anguilla, A. australis, dan A. japonica. Sekuen parsial tersebut secara in silico diketahui mengkode bagian dari protein GH sepanjang 155 asam amino (aa). Sekuen asam amino protein GH A. bicolor sangat mirip dengan sekuen spesies ikan sidat lain dengan hanya tiga perbedaan asam amino dan membentuk satu percabangan pada pohon filogenetik. Tingkat ekspresi gen GH pada sampel ikan sidat memiliki variasi yang tinggi. Hasil penelitian ini dapat menjadi dasar untuk studi selanjutnya khususnya berkaitan dengan peran gen GH dalam pertumbuhan ikan sidat A. bicolor.The growth hormone (GH) gene needs to be studied to support the domestication of the Anguilla bicolor eel in Indonesia. This study aimed to isolate and identify the GH coding gene and analyze its expression in samples of the eel A. bicolor. Primers for amplification of the GH gene of A. bicolor eels were designed based on GH gene sequences from several other eel species available in GenBank. Pituitary and brain cDNA samples of eel were used for GH gene amplification. The amplification results were sequenced and the results were analyzed using BLAST analysis, multiple sequence alignment, hormone signature, and phylogenetic analysis. The GH gene expression analysis was carried out using the real-time PCR technique with the delta-delta CT method on 14 eel samples (23.1 ± 19.6 g; 24.5 ± 4.2 cm). The 486 bp DNA fragment was successfully amplified and sequenced. The GH gene sequence of A. bicolor has a nucleotide similarity of 98.49-99.14% when compared with the GH gene of other eel species A. anguilla, A. australis, and A. japonica. This partial sequence was found in silico to code 155 amino acids (aa) GH protein. The amino acid sequence of the A. bicolor GH protein is very similar to that of other eel species with only three amino acid differences and forms one branch on the phylogenetic tree. The expression level of the GH gene in eel samples had high variations. The results of this study could be a basis for further studies, especially regarding the role of the GH gene in the growth of the eel A. bicolor.

Spatial Distribution Of Heavy Metals Cd And Cu In Water, Sediment And Fish (Mugil Sp) In Pelawangan East Segara Anakan Cilacap: Spatial Distribution Of Heavy Metals Cd And Cu In Water, Sediment And Fish Prayogo, Norman Arie; Hidayati, Nuning Vita; Siregar, Asrul Sahri; Sukardi, Purnama; Fitriadi, Ren
Journal of Aquaculture and Fish Health Vol. 13 No. 2 (2024): JAFH Vol. 13 No. 2 June 2024
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v13i2.46862

Heavy metals are widely known pollutants with bioaccumulative nature, so they can be transferred and accumulated in biota, such as mullet fish (Planiliza subviridis). The heavy metals Cd and Cu have different anthropogenic and natural sources. Heavy metals have the potential to absorb existing biota such mullet fish. Fish absorb metals from the water in the body through gills, skin surface and food. This study aims to identify Cd and Cu metals in distribute water, sediment and mullet fish in the Plawangan Timur, Segara Anakan lagoon, Cilacap, Central Java, and the relationship between aquatic media and sediment and mullet fish. The study used a research method with a targeted random sampling method at five stations with four replications. The survey was conducted from April to September 2020. Descriptive data analysis, F-test, regression and correlation. The results showed in water, sediment and mullet fish the heavy metal content of Cd and Cu varied between stations. The correlation between the Cd and Cu metals in water, sediment and mullet fish showed a positive correlation. The content of heavy metal Cd in mullet fish media in Plawangan Timur Segara Anakan Cilacap has exceeded the specified quality standard, while for heavy metal Cu in mullet fish is still below the specified quality standard.

EKSISTENSI SPESIES IKAN INTRODUKSI PADA HASIL TANGKAPAN NELAYAN JARING INSANG (GILLNET) DI PERAIRAN WADUK PANGLIMA BESAR SOEDIRMAN KABUPATEN BANJARNEGARA Kurnia, Dhimas Ragil; Sukardi, Purnama; Iqbal, Achmad
MARLIN Vol 2, No 1 (2021): (Februari, 2021)
Publisher : Politeknik Kelautan dan Perikanan Pangandaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/marlin.V2.I1.2021.11-20

Waduk Panglima Besar Soedirman, Kabupaten Banjarnegara, merupakan waduk dengan fungsi untuk pembangkit listrik, irigasi pertanian, pariwisata, budidaya dan penangkapan ikan. Jaring Insang (Gillnet) merupakan salah satu alat tangkap yang banyak digunakan oleh nelayan perairan Waduk PB Soedirman. Tujuan dari penelitian ini adalah untuk mengetahui komposisi jenis-jenis hasil tangkapan nelayan jaring insang (gillnet) di Waduk Panglima Besar Soedirman, baik jenis introduksi maupun jenis lokal dan juga untuk mengetahui tingkat dominansi antar spesies dan dominansi antara ikan jenis introduksi dan ikan jenis lokal hasil tangkapan nelayan jaring insang (gillnet) di Waduk Panglima Besar Soedirman. Penelitian ini dilaksanakan pada bulan Juni sampai dengan Agustus 2019. Data jenis-jenis ikan hasil tangkapan nelayan gillnet dianalisis menggunakan rumus komposisi jenis dan rumus indeks dominansi Simpson (C). Jenis-jenis ikan hasil tangkapan nelayan jaring insang (gillnet) di Waduk Panglima Besar Soedirman diantaranya adalah Nila (Oreochromis niloticus) sebanyak 51%; Louhan (Cichlasoma trimaculatum) 21%; Betutu (Oxyeleotris marmorata) 21%; Tawes (Barbonymus gonionotus) 3%; Nilem (Osteochillus vittatus) 2%; Gabus (Channas triata) 1%; Mas (Ciprinus carpio) 1%; dan Bawal (Collosoma macropomum) 0,14%. Jenis-jenis ikan yang termasuk dalam jenis ikan introduksi adalah Nila (Oreochromis niloticus), Louhan (Cichlasoma trimaculatum), Betutu (Oxyeleotris marmorata), Mas (Ciprinus carpio) dan Bawal (Collosoma macropomum) dengan jumlah prosentase 94%. Jenis-jenis ikan yang termasuk ikan lokal adalah Tawes (Barbonymus gonionotus), Nilem (Osteochillus vittatus) dan Gabus (Channas triata) dengan prosentase prosentase 6%. Nilai indeks dominansi (C) antar spesies adalah 0,3538 yang berarti tingkat dominansi sedang dan nilai indeks dominansi (C) antara ikan jenis introduksi dan ikan jenis lokal adalah 0,8981 yang berarti tingkat dominansi tinggi.

PENGARUH FAKTOR-FAKTOR PRODUKSI TERHADAP HASIL TANGKAPAN NELAYAN JARING INSANG (GILLNET) DI PERAIRAN WADUK PANGLIMA BESAR SOEDIRMAN KABUPATEN BANJARNEGARA Kurnia, Dhimas Ragil; Sukardi, Purnama; Iqbal, Achmad
MARLIN Vol 2, No 1 (2021): (Februari, 2021)
Publisher : Politeknik Kelautan dan Perikanan Pangandaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/marlin.V2.I1.2021.31-41

Molecular Cloning of the Vitellogenin Gene in the Hard-Lipped Barb (Osteochillus hasseltii C.V) and Photoperiod’s Effects on Gene Expression Prayogo, Norman Arie; Siregar, Asrul Sahri; Sukardi, Purnama; Bessho, Yasumasa
BIOTROPIA Vol. 25 No. 3 (2018): BIOTROPIA Vol. 25 No. 3 December 2018
Publisher : SEAMEO BIOTROP

Photoperiod affects fish reproduction as it regulates activities of the endocrine glands, which produce the hormones needed for geonadal growth and development, gametogenesis, and reproductive cycles. This study aimed to determine the effects of photoperiod on the hard-lipped barb's reproductive performance by exposing the fish to three photoperiod treatments (light hour: L, darks hour: D), namely 14L:10D (control), 8L:16D (short photoperiod) and 18L:6D (long photoperiod), with four aquaria, each containing 9 fish, serving as replicates. The fish were kept under these photoperiods for 8 weeks. Liver activity, the observable variable in the study, was evaluated by measuring vitellogenin gene expression. Normalized data were then subjected to ANOVA, followed by Tukey's range test. The hard-lipped barb's vitellogenin cDNA was found have a 1136 bp sequence and the vitellogenin precursors encoded cDNA comprising 378 amino acids. The vitellogenin gene in each experimental group saw a significant increase on average when exposed to longer photoperiods (P<0.05), and the highest levels of vitellogenin gene expression occurred under long photoperiods (LP, 18 h light:6 h dark). These results indicate that longer photoperiods stimulate and improve the hard-lipped barb's reproductive performance.

Co-Authors , Hartoyo, , -, Andreas Eka Saputra . HERNAYANTI Abednego Dwi Septiadi Achmad Iqbal Agung Sudaryono Agus Margiwiyatno Agus Nuryanto ahmad, ananda Amron Amron, Amron Anisur Rosyad Arif Yulianto Asrul Sahri Siregar Athari, Hafizha Nur Bessho, Yasumasa Ekasanti, Anandita Eko Setyo Wibowo, Eko Setyo Emyliana Listiowati, Emyliana Endang Hilmi Fitriadi, Ren Frentina Murti Sujadi Harisam, Taufan Harisman, Taufan Haryono, Florencius Eko Dwi Heratri, Agnes Insyira, Athalla Rania Jarot Santoso Kasprijo, Kasprijo Khoerudin, Fatah Kurnia, Dhimas Ragil Kusuma, Baruna Lilik Setiyaningsih Lilik Setiyaningsih Mardika, Hanisya Putri Kania Mardiyana Mardiyana Marhaeni, Bintang Masashi Kawaichi, Masashi Muhammad Riady, Reza Mulandari, Kusriyah Norman Arie Prayogo, Norman Arie Nugrayani, Dewi Nuning Vita Hidayati Nurhabib, Asro nurhafid, mohmammad Nurul Anwar Pramono, Taufik Budi Rijal, Muhammad Azharul Rose Dewi Rosita, Rita Eka Selia Irawati, Maghfira Setyawan, Agung Cahyo Soedibya, Hary Tjahja Soedibya, Petrus Hary Tjahja Sorta Basar Ida Simanjuntak Sri Marnani Susanto Susanto Syakuri, Hamdan Syuraihanah, Khotoh Taufik Budhi Pramono Tjahjo Winanto, Tjahjo Tobari Tohap Simangunsong Trianingtyas, Laela Wahyu Andi Saputra Wibowo, Satrio Haryu Wijaya, Rudy Winanto, Tjahyo Yansah, Noprie Yohanes Harvinda Yunida, Rizka Yusup, Dandi Diana

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