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Purnama Sukardi
Fakultas Perikanan dan Ilmu Kelautan Universitas Jenderal Soedirman
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Evaluasi Potensi Tepung Tubifex sebagai Pengganti Tepung Ikan dalam Pakan Benih Ikan Nila (Oreochromis niloticus) Yusup, Dandi Diana; Simangunsong, Tohap; Sukardi, Purnama
Jurnal Perikanan Unram Vol 15 No 4 (2025): JURNAL PERIKANAN
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jp.v15i4.1730

Abstract

The high cost and limited availability of fishmeal in aquaculture feed formulations have prompted the search for sustainable and affordable alternative protein sources. This study aimed to evaluate the effects of substituting fishmeal with silk worm (Tubifex sp.) meal on the growth performance, feed utilization, and survival rate of Nile tilapia (Oreochromis niloticus) fry. A completely randomized design (CRD) was used with four dietary treatments: P1 (control, 0% Tubifex), P2 (15%), P3 (25%), and P4 (35%), each with three replications. Fish were fed twice daily for 30 days, and growth parameters were measured weekly. Results showed that the inclusion of Tubifex meal significantly improved the final weight, specific growth rate (SGR), average daily gain (ADG), and feed efficiency (FE), particularly in P3 and P4. The highest SGR (2.28%/day), ADG (0.016 g/day), and FE (61.93%) were recorded in the P4 group. Survival rate also improved, reaching 82.22% in P4 compared to 51.11% in the control. Water quality parameters remained within optimal ranges, indicating no adverse environmental impact from Tubifex inclusion. The findings suggest that Tubifex meal is a viable protein source that can replace fishmeal by up to 35% in tilapia fry diets. Its application offers nutritional, economic, and ecological benefits, making it a promising component in sustainable aquafeed development.
The Effect of Encapsulator Machine Speed ​​on Composite Microcapsule Size Insyira, Athalla Rania; Athari, Hafizha Nur; Sukardi, Purnama; Wijaya, Rudy
Journal Of Artha Biological Engineering Vol. 2 No. 2 (2024)
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/s0btxz58

Abstract

Microcapsules are small round particles with uniform walls or layers around them that generally have a diameter from 0.2 to 5000 µm. Microencapsulation is a process that converts solid or liquid materials into capsule form which aims to protect and maintain active ingredients from environmental influences such as humidity, high temperature, light exposure, or oxidation processes. Microcapsule formation can be done using the crosslink method. Microcapsule size is affected by the stirring speed of the encapsulator machine. During microencapsulation of fish meal with eggs and gelatin using the crosslink method, the stirring speed is 3000 rpm, 3500 rpm, 4000 rpm, 4500 rpm, and 5000 rpm. In the microencapsulation process of fish meal with eggs and gelatin, the smallest average diameter at a speed of 5000 rpm, which is 18.220 ± 3.3 µm and has a yield of 26% while the largest diameter at a speed of 3000 rpm, which is 33.53 ± 4.8 µm and has a yield of 28.4%. Based on the results of the Tukey SPSS test, the speeds of 3000 rpm (33.53 ± 4.8 µm), 3500 rpm (30.25 ± 4.4 µm), 4000 rpm (26.30 ± 3.5 µm), 4500 rpm (22.50 ± 4.1 µm), and 5000 rpm (18.220 ± 3.3 µm) were very significantly different (P <0.05) in each treatment.
The Effects of Substitution on Fish Meal with Maggot Meal in Artificial Feedfor Eels (Anguilla bicolor) Growth and Survival Rate ahmad, ananda; Sukardi, Purnama; Nugrayani, Dewi; Trianingtyas, Laela
Journal Of Artha Biological Engineering Vol. 3 No. 1 (2025)
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/fwrbjv08

Abstract

Eel (Anguilla sp.) is a leading freshwater fish commodity. In the intensivesystem, high stocking density results in high production costs. Maggot meal isan alternative raw material that has complete nutritional value such as fishmeal but is cheaper. The purpose of this study was to determine theeffectiveness of substituted fish meal with maggot meal on the growth andsurvival rate (Anguilla bicolor) Elver phase. The method carried out in thisstudy used an experimental method with a completely randomized design(CRD) consisting of 4 treatments and 3 replications with a combination of fishmeal and maggot meal, P0: 100% TI and 0% TM, P1: 80% TI and 20% TM,P2: TI 60% and TM 40% and P3: TI 40% and TM 60%. The results of rearingfor 60 days showed a significant effect on absolute weight growth, specificgrowth rate, and yield, while the yield had no significant effect on absolutelength growth, feed conversion, and survival. The results of the water qualityparameters of the media are temperatures ranging from 24 °C -29,8 °C, pHranging from 5,14-7,7, DO ranging from 2,4 mg/l – 5,1 mg/l and ammoniaranging from 0 ppm – 0,01 ppm.
ISOLASI, IDENTIFIKASI, DAN ANALISIS EKSPRESI GEN PENGKODE GROWTH HORMONE PADA IKAN SIDAT (Anguilla bicolor) Syakuri, Hamdan; Wibowo, Satrio Haryu; Ekasanti, Anandita; Soedibya, Petrus Hary Tjahja; Marnani, Sri; Listiowati, Emyliana; Nugrayani, Dewi; Sukardi, Purnama
Jurnal Riset Akuakultur Vol 19, No 1 (2024): (Maret 2024)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.19.1.2024.69-83

Abstract

Gen hormon pertumbuhan (growth hormone, GH) perlu dipelajari untuk mendukung domestikasi ikan sidat Anguilla bicolor di Indonesia. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi gen pengkode GH serta menganalisis ekspresinya pada sampel ikan sidat A. bicolor. Primer untuk amplifikasi gen GH ikan sidat A. bicolor didesain berdasarkan sekuen gen GH dari beberapa spesies ikan sidat lain yang tersedia di GenBank. Sampel cDNA hipofisa dan otak ikan sidat digunakan untuk amplifikasi gen GH. Hasil amplifikasi disekuensing dan hasilnya dianalisis menggunakan analisis BLAST, multiple sequences alignment, hormon signature, dan filogenetik. Analisis ekspresi gen GH dilakukan menggunakan teknik realtime PCR dengan metode delta-delta CT pada 14 sampel ikan sidat (23,1 ± 19,6 g; 24,5 ± 4,2 cm). Fragmen DNA sepanjang 486 bp berhasil diamplifikasi dan disekuensing. Sekuen gen GH A. bicolor memiliki similaritas nukleotida sebesar 98,49-99,14% jika dibandingkan dengan gen GH ikan sidat A. anguilla, A. australis, dan A. japonica. Sekuen parsial tersebut secara in silico diketahui mengkode bagian dari protein GH sepanjang 155 asam amino (aa). Sekuen asam amino protein GH A. bicolor sangat mirip dengan sekuen spesies ikan sidat lain dengan hanya tiga perbedaan asam amino dan membentuk satu percabangan pada pohon filogenetik. Tingkat ekspresi gen GH pada sampel ikan sidat memiliki variasi yang tinggi. Hasil penelitian ini dapat menjadi dasar untuk studi selanjutnya khususnya berkaitan dengan peran gen GH dalam pertumbuhan ikan sidat A. bicolor.The growth hormone (GH) gene needs to be studied to support the domestication of the Anguilla bicolor eel in Indonesia. This study aimed to isolate and identify the GH coding gene and analyze its expression in samples of the eel A. bicolor. Primers for amplification of the GH gene of A. bicolor eels were designed based on GH gene sequences from several other eel species available in GenBank. Pituitary and brain cDNA samples of eel were used for GH gene amplification. The amplification results were sequenced and the results were analyzed using BLAST analysis, multiple sequence alignment, hormone signature, and phylogenetic analysis. The GH gene expression analysis was carried out using the real-time PCR technique with the delta-delta CT method on 14 eel samples (23.1 ± 19.6 g; 24.5 ± 4.2 cm). The 486 bp DNA fragment was successfully amplified and sequenced. The GH gene sequence of A. bicolor has a nucleotide similarity of 98.49-99.14% when compared with the GH gene of other eel species A. anguilla, A. australis, and A. japonica. This partial sequence was found in silico to code 155 amino acids (aa) GH protein. The amino acid sequence of the A. bicolor GH protein is very similar to that of other eel species with only three amino acid differences and forms one branch on the phylogenetic tree. The expression level of the GH gene in eel samples had high variations. The results of this study could be a basis for further studies, especially regarding the role of the GH gene in the growth of the eel A. bicolor. 
Spatial Distribution Of Heavy Metals Cd And Cu In Water, Sediment And Fish (Mugil Sp) In Pelawangan East Segara Anakan Cilacap: Spatial Distribution Of Heavy Metals Cd And Cu In Water, Sediment And Fish Prayogo, Norman Arie; Hidayati, Nuning Vita; Siregar, Asrul Sahri; Sukardi, Purnama; Fitriadi, Ren
Journal of Aquaculture and Fish Health Vol. 13 No. 2 (2024): JAFH Vol. 13 No. 2 June 2024
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v13i2.46862

Abstract

Heavy metals are widely known pollutants with bioaccumulative nature, so they can be transferred and accumulated in biota, such as mullet fish (Planiliza subviridis). The heavy metals Cd and Cu have different anthropogenic and natural sources. Heavy metals have the potential to absorb existing biota such mullet fish. Fish absorb metals from the water in the body through gills, skin surface and food. This study aims to identify Cd and Cu metals in distribute water, sediment and mullet fish in the Plawangan Timur, Segara Anakan lagoon, Cilacap, Central Java, and the relationship between aquatic media and sediment and mullet fish. The study used a research method with a targeted random sampling method at five stations with four replications. The survey was conducted from April to September 2020. Descriptive data analysis, F-test, regression and correlation. The results showed in water, sediment and mullet fish the heavy metal content of Cd and Cu varied between stations. The correlation between the Cd and Cu metals in water, sediment and mullet fish showed a positive correlation. The content of heavy metal Cd in mullet fish media in Plawangan Timur Segara Anakan Cilacap has exceeded the specified quality standard, while for heavy metal Cu in mullet fish is still below the specified quality standard.
EKSISTENSI SPESIES IKAN INTRODUKSI PADA HASIL TANGKAPAN NELAYAN JARING INSANG (GILLNET) DI PERAIRAN WADUK PANGLIMA BESAR SOEDIRMAN KABUPATEN BANJARNEGARA Kurnia, Dhimas Ragil; Sukardi, Purnama; Iqbal, Achmad
MARLIN Vol 2, No 1 (2021): (Februari, 2021)
Publisher : Politeknik Kelautan dan Perikanan Pangandaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/marlin.V2.I1.2021.11-20

Abstract

Waduk Panglima Besar Soedirman, Kabupaten Banjarnegara, merupakan waduk dengan fungsi untuk pembangkit listrik, irigasi pertanian, pariwisata, budidaya dan penangkapan ikan. Jaring Insang (Gillnet) merupakan salah satu alat tangkap yang banyak digunakan oleh nelayan perairan Waduk PB Soedirman. Tujuan dari penelitian ini adalah untuk mengetahui komposisi jenis-jenis hasil tangkapan nelayan jaring insang (gillnet) di Waduk Panglima Besar Soedirman, baik jenis introduksi maupun jenis lokal dan juga untuk mengetahui tingkat dominansi antar spesies dan dominansi antara ikan jenis introduksi dan ikan jenis lokal hasil tangkapan nelayan jaring insang (gillnet) di Waduk Panglima Besar Soedirman. Penelitian ini dilaksanakan pada bulan Juni sampai dengan Agustus 2019. Data jenis-jenis ikan hasil tangkapan nelayan gillnet dianalisis menggunakan rumus komposisi jenis dan rumus indeks dominansi Simpson (C). Jenis-jenis ikan hasil tangkapan nelayan jaring insang (gillnet) di Waduk Panglima Besar Soedirman diantaranya adalah Nila (Oreochromis niloticus) sebanyak 51%; Louhan (Cichlasoma trimaculatum) 21%; Betutu (Oxyeleotris marmorata) 21%; Tawes (Barbonymus gonionotus) 3%; Nilem (Osteochillus vittatus) 2%; Gabus (Channas triata) 1%; Mas (Ciprinus carpio) 1%; dan Bawal (Collosoma macropomum) 0,14%. Jenis-jenis ikan yang termasuk dalam jenis ikan introduksi adalah Nila (Oreochromis niloticus), Louhan (Cichlasoma trimaculatum), Betutu (Oxyeleotris marmorata), Mas (Ciprinus carpio) dan Bawal (Collosoma macropomum) dengan jumlah prosentase 94%. Jenis-jenis ikan yang termasuk ikan lokal adalah Tawes (Barbonymus gonionotus), Nilem (Osteochillus vittatus) dan Gabus (Channas triata) dengan prosentase prosentase 6%.  Nilai indeks dominansi (C) antar spesies adalah 0,3538 yang berarti tingkat dominansi sedang dan nilai indeks dominansi (C) antara ikan jenis introduksi dan ikan jenis lokal adalah 0,8981 yang berarti tingkat dominansi tinggi.
PENGARUH FAKTOR-FAKTOR PRODUKSI TERHADAP HASIL TANGKAPAN NELAYAN JARING INSANG (GILLNET) DI PERAIRAN WADUK PANGLIMA BESAR SOEDIRMAN KABUPATEN BANJARNEGARA Kurnia, Dhimas Ragil; Sukardi, Purnama; Iqbal, Achmad
MARLIN Vol 2, No 1 (2021): (Februari, 2021)
Publisher : Politeknik Kelautan dan Perikanan Pangandaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/marlin.V2.I1.2021.31-41

Abstract

Waduk Panglima Besar Soedirman, Kabupaten Banjarnegara, merupakan waduk dengan fungsi untuk pembangkit listrik, irigasi pertanian, pariwisata, budidaya dan penangkapan ikan. Jaring Insang (Gillnet) merupakan salah satu alatv tangkap yang banyak digunakan oleh nelayan perairan Waduk PB Soedirman. Tujuan dari penelitian ini adalah untuk mengetahui faktor-faktor produksi penangkapan ikan menggunakan gillnet dan pengaruhnya terhadap hasil tangkapan nelayan. Penelitian ini dilaksanakan pada bulan Juni sampai dengan Agustus 2019. Data faktor-faktor produksi penangkapan ikan dan hasil tangkapan dianalisis menggunakan rumus fungsi produksi Cobb-Douglas untuk mengetahui pengaruh faktor-faktor produksi terhadap hasil tangkapan nelayan. Faktor-faktor produksi penangkapan ikan menggunakan gillnet yaitu panjang jaring (X1), lebar mata jaring (X3), lama waktu penangkapan (X4), umur nelayan (X5) dan pengalaman nelayan (X6), secara bersama-sama memberikan pengaruh signifikan terhadap hasil tangkapan nelayan (Y), pada tingkat kepercayaan 95%. Secara parsial atau satu per satu, pada tingkat kepercayaan 95%, hanya panjang jaring (X1), lebar mata jaring (X3), lama waktu penangkapan (X4) dan pengalaman nelayan (X6) yang memberikan pengaruh signifikan terhadap hasil tangkapan nelayan (Y).
Implementasi Inovasi Probiotik Berbasis Limbah Pasar untuk Meningkatkan Produktivitas Ikan Nila di Kelompok Krido Yuwono Mina Padi Technopark, Panembangan, Banyumas Sujadi, Frentina Murti; Anjaini, Jefri; Simangunsong, Tohap; Setiyaningsih, Lilik; Mardika, Hanisya Putri Kania; Setyawan, Agung Cahyo; Sukardi, Purnama; Kusuma, Baruna; Nurhabib, Asro; Marnani, Sri; Harvinda, Yohanes
Jurnal Pengabdian Magister Pendidikan IPA Vol 8 No 4 (2025): Oktober-Desember 2025
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpmpi.v8i4.13355

Abstract

The probiotic innovation training programme based on market waste implemented at the Krido Yuwono Mina Padi Technopark Group aims to increase tilapia productivity and farming efficiency through the use of organic waste as raw material for probiotics. This training involves participants in the production of probiotics through the fermentation of market waste using lactic acid bacteria starters. The evaluation results show an increase in tilapia productivity of around 15-20% with better feed efficiency and a reduction in ammonia levels in pond water, which was previously a major problem in maintaining fish health. The community involved in this programme also increased their confidence in managing tilapia farming efficiently and received new knowledge that can reduce operational costs. This programme has achieved significant social and economic impacts and is expected to develop into a sustainable community empowerment model in the field of aquaculture and support more environmentally friendly waste management.
Edukasi Penerapan Autofeeder dan Manajemen Kualitas Air Berbasis Energi Surya pada Budidaya Minapadi Desa Penembangan, Banyumas Anjaini, Jefri; Nurhabib, Asro; Sukardi, Purnama; Setiyaningsih, Lilik; Ekowati, Nur Alfi; Agung Tri Nugroho
Jurnal Pengabdian Magister Pendidikan IPA Vol 8 No 4 (2025): Oktober-Desember 2025
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpmpi.v8i4.13785

Abstract

POKDAKAN (Kelompok Pembudidaya Ikan) memiliki peran penting dalam pengembangan budidaya perikanan berkelanjutan, termasuk sistem integrasi minapadi. Namun, tantangan yang dihadapi adalah keterbatasan energi listrik di area persawahan serta pemberian pakan yang masih manual, yang menyebabkan efisiensi produksi belum optimal. Kegiatan pengabdian masyarakat ini dilaksanakan pada tanggal 20 September 2025 di Desa Penembangan, Banyumas, Jawa Tengah oleh tim dosen Fakultas Perikanan dan Ilmu Kelautan Universitas Jenderal Soedirman. Tujuan kegiatan adalah meningkatkan kapasitas POKDAKAN melalui edukasi, pelatihan, pendampingan, dan transfer pengetahuan dalam penerapan sistem otomatisasi pakan berbasis energi surya. Metode yang digunakan meliputi sosialisasi, edukasi, wawancara, kuesioner, serta replikasi teknologi. Hasil kegiatan menunjukkan peningkatan pengetahuan dan keterampilan anggota POKDAKAN, peningkatan efisiensi pakan sebesar 20–25%, serta kesiapan pembudidaya untuk mereplikasi sistem di lahan lain. Program ini membuktikan bahwa edukasi berbasis teknologi dapat meningkatkan kemandirian pembudidaya sekaligus mendukung praktik budidaya ramah lingkungan.
Penerapan Auto Feeder dan Sistem Monitoring Kualitas Air Real-Time Berbasis Tenaga Surya pada Budidaya Minapadi Terintegrasi POKDAKAN Kridoyuwono, Panembangan, Banyumas Sukardi, Purnama; Anjaini, Jefri; Lilik Setiyaningsih; Nur Alfi Ekowati; Agung Tri Nugroho; Frentina Murti Sujadi; Asro Nurhabib; Baruna Kusuma; Yohannes Harvinda; Kholilah Nur Hidayah; Dandi Setio Wibowo; Dimas Yoland Dewantio
Jurnal Pengabdian Magister Pendidikan IPA Vol 8 No 4 (2025): Oktober-Desember 2025
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpmpi.v8i4.13857

Abstract

Panembangan Village has strong potential for rice–fish culture (minapadi) but faces constraints in feeding efficiency, unmonitored water quality, and dependence on grid electricity. This community‐service program offers an integrated solution: an IoT-based auto feeder combined with real-time water-quality monitoring (DO, pH, temperature, conductivity/TDS, turbidity) and powered by off-grid solar energy (PV) to ensure continuous operation. The primary goal was to improve feed efficiency, enhance fish growth, reduce feed waste, and optimize water quality management in aquaculture systems. The implementation of these technologies has demonstrated significant improvements in feed conversion ratios, growth rates of fish, and overall water quality management. The program also provided training to local farmers, enhancing their capacity to manage these technologies effectively. This initiative is expected to contribute to the sustainable development of aquaculture in the region, benefiting both the local community and the environment
Co-Authors , Hartoyo, , . HERNAYANTI Abednego Dwi Septiadi Achmad Iqbal Agung Sudaryono Agung Tri Nugroho Agus Margiwiyatno Agus Nuryanto ahmad, ananda Anisur Rosyad Arif Yulianto Asrul Sahri Siregar Athari, Hafizha Nur Dandi Setio Wibowo Dimas Yoland Dewantio Ekasanti, Anandita Eko Setyo Wibowo, Eko Setyo Emyliana Listiowati, Emyliana Endang Hilmi Fitriadi, Ren Frentina Murti Sujadi Harisman, Taufan Insyira, Athalla Rania Jarot Santoso Khoerudin, Fatah Kholilah Nur Hidayah Kurnia, Dhimas Ragil Kusuma, Baruna Lilik Setiyaningsih Lilik Setiyaningsih Mardika, Hanisya Putri Kania Mardiyana Mardiyana Mulandari, Kusriyah Norman Arie Prayogo, Norman Arie Nugrayani, Dewi Nuning Vita Hidayati Nur Alfi Ekowati Nurhabib, Asro Nurul Anwar Rijal, Muhammad Azharul Rose Dewi Rosita, Rita Eka Setyawan, Agung Cahyo Soedibya, Hary Tjahja Soedibya, Petrus Hary Tjahja Sorta Basar Ida Simanjuntak Sri Marnani Susanto Susanto Syakuri, Hamdan Syuraihanah, Khotoh Taufik Budhi Pramono Tjahjo Winanto, Tjahjo Tobari Tohap Simangunsong Trianingtyas, Laela Wahyu Andi Saputra Wibowo, Satrio Haryu Wijaya, Rudy Yohanes Harvinda Yunida, Rizka Yusup, Dandi Diana