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Eko Agus Srihanto, Eko Agus
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Detection of Environmental DNA from Tree Bark Associated with Rubbing Behavior of Dicerorhinus sumatrensis in Way Kambas National Park Priyambodo; Zulkarnain, Danisworo; Rustiati, Elly Lestari; Kurniawati, Yeyen; Putri, Chicka Refina Rahma; Srihanto, Eko Agus; Saswiyanti, Enny; Arsan, Zulfi; Sukatmoko; Pratiwi, Dian Neli; Permatasari, Nindy
ORGANISMS: JOURNAL OF BIOSCIENCES Vol. 6 No. 1 (2026): Organisms: Journal of Biosciences
Publisher : Pusat HKI, Paten, dan Publikasi Ilmiah Universitas Islam Negeri Raden Intan Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/zv2m2645

Abstract

The development of non-invasive methods is essential for monitoring critically endangered species such as the Dicerorhinus sumatrensis. This study aimed to evaluate the potential of tree bark associated with rubbing behavior as a source of environmental DNA (eDNA) for species detection in Way Kambas National Park. A total of 30 samples were collected from tree bark surfaces showing evidence of rubbing activity. DNA was extracted and analyzed using two approaches: simple electrophoresis and polymerase chain reaction (PCR)-based molecular methods. The results showed that the simple method failed to detect visible DNA bands, indicating low DNA concentration and degradation in environmental samples. In contrast, PCR amplification successfully detected DNA in 5 out of 30 samples, including hair-derived and epithelial residues associated with rubbing behavior.containing hair residues produced more reliable detection compared to those with only epithelial traces. These findings demonstrate that tree bark able to serve as a potential non-invasive source of eDNA, particularly when analyzed using sensitive molecular techniques. This approach provides an alternative method for detecting elusive species and contributes to the development of effective conservation strategies for the sumatran rhinoceros.
Co-Authors Adelia, Ersa Imelda Afandi, Aril Alfandi, Aril Anggy, Fransiska Panasea Arsan, Zulfi Ashari, Minanti Mayda Azmi, Sevira Nur Azzahra, Laila Salwa Elly Lestari Rustiati Fahrezi, Aditya Febriansyah, Muhammad Febriyani, Dwi Ayu Firwantoni, Firwantoni Gina Dania Pratami, Gina Dania Heni, Ahyul Imtitsal, Aulia Indah Ayu Lestari, Indah Ayu Jani Master, Jani Krismuniarti, Elisabeth Devi Kurniasih, Vidyanti Kurniawati, Yeyen Kusuma, Andriyani Wijaya Lestari, Septi Wahyu Maharani, Annisa Lidya Michael Haryadi Wibowo Neli Pratiwi, Dian Novianasari, Tika Panasea Anggy, Fransiska Permatasari, Nindy Pratiwi, Dian Neli Priyambodo Priyambodo Priyambodo Priyambodo Priyambodo Putri, Chicka Refina Rahma Raden Mohamad Herdian Bhakti Ramadan, Viki Ramadhan, Viki Rhamadaningtyas, Nabila Aulia Rustiaati, Elly Lestari Sa'uddah, Lousanja D. Sandra, Shifa Saswiyanti, Enny Sidik, Mahfud Suhada Suhada, Suhada Sukatmoko Susandi, Waryoko Susanto, Alvin Wiwiet Thesalonika, Natasya Virnarenata, Elsa Widya Asmara WINARNO Winarno Winarno Yani, Ani Andri Yanti Yulianti Yuliana Andriyani Yunior, Elfita Nova Zulkarnain, Danisworo